Environment-friendly plant fiber degumming agent, preparation method and application thereof

Abstract

The application provides an environment-friendly plant fiber degumming agent and a preparation method and application thereof, and the environment-friendly plant fiber degumming agent comprises the following raw materials in parts by weight: streptomyces lydicus bacterial liquid 20-40 parts, lemon grass extract 20-30 parts, degumming aid 5-10 parts, complexing agent 5-10 parts, surfactant 20-30 parts and inorganic salt 5-10 parts. The preparation method of the environment-friendly plant fiber degumming agent comprises the following steps: 1) the complexing agent, the degumming aid, the inorganic salt, the surfactant and the lemon grass extract are added, heated to 20-30 DEG C, and uniformly stirred; 2) the streptomyces lydicus bacterial liquid is added into the filtrate obtained in step 1), and uniformly stirred and mixed, so that the environment-friendly plant fiber degumming agent is obtained. The environment-friendly plant fiber degumming agent is environment-friendly, has a degumming effect, and has a good application prospect in fiber production.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant fiber production, and particularly relates to an environment-friendly plant fiber degumming agent and a preparation method and application thereof. BACKGROUND

[0002] Natural plant fiber is one of the most abundant renewable resources in nature, and has the characteristics of biodegradability and environmental friendliness. The main components of natural plant fiber, cellulose, hemicellulose and lignin macromolecular structure, contain functional groups that can participate in chemical reactions, and can be prepared into a variety of functional materials, such as high water absorption materials, heavy metal ion adsorption materials, medical and health materials, and widely used in textile, papermaking and composite materials fields. For example, banana stems, pineapple leaves and other agricultural wastes can be used as raw materials for fiber production, avoiding environmental pollution and resource waste caused by discarding in the field or burning in the past.

[0003] The process of raw materials such as banana stems and pineapple leaves in preparing fibers generally includes scraping, washing, degumming and combing processes. Degumming treatment of fibers can ensure better utilization in textile industry, and the quality of degumming largely determines the quality of plant fibers.

[0004] Traditional degumming process is a chemical degumming process with caustic soda as the core. Due to the use of strong acid soaking and secondary high temperature, high pressure and strong alkali boiling, the spinnability of the fiber is deteriorated, and the earth environment is seriously polluted. At the same time, the fabric is damaged to a certain extent. With the enhancement of environmental protection concept, many people in the industry believe that the use of biological degumming technology or the combination of biological and chemical degumming technology can not only overcome the defects of traditional chemical degumming method, but also improve the degumming rate, which is a new way of degumming.

[0005] Therefore, in order to meet the needs of practical application, it is necessary to provide an environment-friendly plant fiber degumming agent with low cost, environmental friendliness and good degumming effect. SUMMARY

[0006] In view of the problems in the prior art, the purpose of the present application is to provide an environment-friendly plant fiber degumming agent with low cost, environmental friendliness and good degumming effect, so as to better meet the needs of practical application.

[0007] To achieve this purpose, the technical scheme of the present application is as follows:

[0008] The present application provides an environment-friendly plant fiber degumming agent, which comprises the following raw materials by weight: Streptomyces lydicus liquid 20-40 parts, lemon extract 20-30 parts, degumming aid 5-10 parts, complexing agent 5-10 parts, surfactant 20-30 parts and inorganic salt 5-10 parts.

[0009] In some embodiments of the present application, the preparation method of the Streptomyces lydicus liquid culture comprises the following steps:

[0010] (1) picking Streptomyces lydicus to inoculate into a culture medium, and activating on a shaker to obtain an activated strain;

[0011] (2) inoculating the activated strain into a liquid culture medium to obtain a Streptomyces lydicus liquid culture.

[0012] In the preparation method of the Streptomyces lydicus liquid culture of the present application, the composition of the culture medium in step (1) is: sucrose 3-5 g / L, glucose 10-20 g / L, soluble starch 1-3 g / L, citric acid diammonium oxalate 1-3 g / L, sodium acetate 5-8 g / L, magnesium sulfate 5-10 g / L, manganese sulfate 2-5 g / L, and agar 20-25 g / L; the formula of the liquid culture medium is: tryptone 5-15 g / L, sodium chloride 8-12 g / L, yeast extract 3-5 g / L, sodium sulfate 3-5 g / L, and deionized water 0.5-1 L.

[0013] In the preparation method of the Streptomyces lydicus liquid culture of the present application, the shaker activation temperature in step (1) is 30-35°C (for example, it can be 30°C, 30.5°C, 31°C, 31.5°C, 32°C, 32.5°C, 33°C, 33.5°C, 34°C, 34.5°C, 35°C, or any temperature value therein), and the shaker activation time is 12-16 h (for example, it can be 12 h, 12.5 h, 13 h, 13.5 h, 14 h, 14.5 h, 15 h, 15.5 h, 16 h, or any value therein).

[0014] In the preparation method of the Streptomyces lydicus liquid culture of the present application, the culture temperature in step (2) is 25-35°C (for example, it can be 25°C, 25.5°C, 26°C, 26.5°C, 27°C, 27.5°C, 28°C, 28.5°C, 29°C, 29.5°C, 30°C, 30.5°C, 31°C, 31.5°C, 32°C, 32.5°C, 33°C, 33.5°C, 34°C, 34.5°C, 35°C, or any temperature value therein), and the culture time is 48-72 h (for example, it can be 48 h, 49 h, 50 h, 51 h, 52 h, 53 h, 54 h, 55 h, 56 h, 57 h, 58 h, 59 h, 60 h, 61 h, 62 h, 63 h, 64 h, 65 h, 66 h, 67 h, 68 h, 69 h, 70 h, 71 h, 72 h, or any value therein).

[0015] In some embodiments of the present application, the preparation method of the citronella extract comprises the following steps:

[0016] (1) The lemon grass is washed and then dried, then crushed, and the powder is added to distilled water and a composite enzyme;

[0017] (2) The product obtained in step (1) is distilled, the distillate is collected, and the oil obtained after oil-water separation is the lemon grass extract.

[0018] In the preparation method of the lemon grass extract, the distilled water in step (1) is 3-8 times (for example, it can be 3 times, 4 times, 5 times, 6 times, 7 times, 9 times, or any multiple value) the mass of the lemon grass; the composite enzyme includes pectinase, ligninase, and hemicellulase, and the mass ratio of pectinase, ligninase, and hemicellulase is 1:2-3:2-3 (for example, it can be 1:2:2, 1:2.5:2, 1:3:2, 1:2:2.5, 1:2.5:2.5, 1:3:2.5, 1:2:3, 1:2.5:3, 1:3:3, or any value); the mass of the composite enzyme is 10%-15% (for example, it can be 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, or any value) of the lemon grass.

[0019] In some embodiments of the present application, the inorganic salt is selected from one or more combinations of sodium sulfite, sodium formate, potassium chloride, sodium chloride, and calcium chloride.

[0020] In some embodiments of the present application, the degumming aid is selected from sodium silicate or sodium tripolyphosphate.

[0021] In some embodiments of the present application, the complexing agent is selected from one or more combinations of triethanolamine, monoethanolamine, and diethanolamine.

[0022] In some embodiments of the present application, the surfactant is selected from one or more combinations of sodium lauroyl sarcosinate, sodium cocoyl glutamate, sodium lauryl glutamate, sodium lauroyl glycinate, and sodium cocoyl sarcosinate.

[0023] A preparation method of an environmentally friendly plant fiber degumming agent, the preparation method of the environmentally friendly plant fiber degumming agent comprising the following steps:

[0024] (1) The complexing agent, degumming aid, inorganic salt, and surfactant are added to the lemon grass extract, heated to 20-30°C, and stirred uniformly;

[0025] (2) The filtrate obtained in step (1) is added to the Streptomyces lydicus bacterial solution, and stirred and mixed uniformly to obtain the environmentally friendly plant fiber degumming agent.

[0026] The application also provides an application mode of the above-mentioned environment-friendly plant fiber degumming agent in a plant fiber degumming process, and the use conditions of the environment-friendly plant fiber degumming agent in degumming are as follows: a degumming temperature is 30-40 DEG C (for example, it can be 30 DEG C, 30.5 DEG C, 31 DEG C, 31.5 DEG C, 32 DEG C, 32.5 DEG C, 33 DEG C, 33.5 DEG C, 34 DEG C, 34.5 DEG C, 35 DEG C, 36 DEG C, 36.5 DEG C, 37 DEG C, 37.5 DEG C, 38 DEG C, 38.5 DEG C, 39 DEG C, 39.5 DEG C, 40 DEG C or any temperature value therein), a pressure is 20-25 MPa (for example, it can be 20 MPa, 21 MPa, 22 MPa, 23 MPa, 24 MPa, 25 MPa or any value therein), and a degumming time is 20-30 min (for example, it can be 20 min, 21 min, 22 min, 23 min, 24 min, 25 min, 26 min, 27 min, 28 min, 29 min, 30 min or any value therein).

[0027] Compared with the prior art, the application has the following beneficial effects:

[0028] (1) The environment-friendly plant fiber degumming agent provided by the application has excellent degumming effect, and the refined dry jute after degumming has excellent mechanical properties, high fiber production rate and less by-products, and the process reaction conditions are mild, so that the use of a large amount of toxic and harmful chemical reagents is avoided, thereby reducing the discharge of waste liquid, reducing environmental pollution and wastewater treatment cost, and having the advantages of low cost, no pollution to the environment and good degumming effect.

[0029] (2) The environment-friendly plant fiber degumming agent provided by the application can better adapt to the complex components of gum by using lemon extract and adding composite enzymes in the preparation process, and can specifically degum and degrade the lignin in the firm middle lamella to make the fibers resolve out, so that the gum can be better degraded. The lemon extract is rich in tannin, flavone, phenolic substance and alkaloid, can keep the streptomyces lydicus liquid in good activity and stability, and will not be inactivated in the process of degrading lignin, and can synergistically degrade the gum. DETAILED DESCRIPTION

[0030] The application will be described below in combination with specific embodiments. It should be noted that the following examples are only used to illustrate the application, and are not used to limit the application. Other combinations and various modifications within the concept of the application can be made without departing from the main idea or scope of the application. In the following examples, the compounds and related reagents used can be purchased from the market.

[0031] Preparation of streptomyces lydicus liquid I

[0032] (1) pick up streptomyces lvidans inoculation to the culture medium, in 35℃ under the shaking table activation 16h, prepared the activation strain;

[0033] (2) the strain after activation is inoculated in liquid culture medium, in 35℃ under the scale-up culture, culture 72h, get streptomyces lvidans broth I.

[0034] The culture medium ingredients are: sucrose 5g / L, glucose 20g / L, soluble starch 3g / L, diammonium hydrogen citrate 3g / L, sodium acetate 8g / L, magnesium sulfate 10g / L, manganese sulfate 5g / L, agar 25g / L.

[0035] The formula of the liquid culture medium is: tryptone 15g / L, sodium chloride 12g / L, yeast extract 5g / L, sodium sulfate 3-5L, deionized water 1L;

[0036] Preparation of streptomyces lvidans broth II

[0037] (1) pick up streptomyces lvidans inoculation to the culture medium, in 30℃ under the shaking table activation 12h, prepared the activation strain;

[0038] (2) the strain after activation is inoculated in liquid culture medium, in 25℃ under the scale-up culture, culture 48h, get streptomyces lvidans broth II;

[0039] The culture medium ingredients are: sucrose 3g / L, glucose 10g / L, soluble starch 1g / L, diammonium hydrogen citrate 1g / L, sodium acetate 5g / L, magnesium sulfate 5g / L, manganese sulfate 2g / L, agar 20g / L;

[0040] The formula of the liquid culture medium is: tryptone 5g / L, sodium chloride 8g / L, yeast extract 3g / L, sodium sulfate 3g / L, deionized water 0.5L.

[0041] Preparation of lemon grass extract I

[0042] (1) 100g of lemon grass is washed and dried, then crushed, and the powder is added to 800g of distilled water and 15g of complex enzyme (the mass ratio of pectinase, ligninase and hemicellulase is 1:3:3);

[0043] (2) the product obtained in step (1) is distilled, the distillate is collected, and the oil obtained after oil-water separation is the lemon grass extract.

[0044] Preparation of lemon grass extract II

[0045] (1) 100g lemon grass is cleaned and dried, then crushed, and the powder is added to 300g distilled water and 10g of a composite enzyme (the mass ratio of pectinase, ligninase and hemicellulase is 1:2:2);

[0046] (2) The product obtained in step (1) is distilled, and the distillate is collected. The oil obtained after oil-water separation is the lemon grass extract.

[0047] Preparation of lemon grass extract III

[0048] (1) 100g lemon grass is cleaned and dried, then crushed, and the powder is added to 300g distilled water;

[0049] (2) The product obtained in step (1) is distilled, and the distillate is collected. The oil obtained after oil-water separation is the lemon grass extract.

[0050] Example 1

[0051] An environmentally friendly plant fiber degumming agent, the environmentally friendly plant fiber degumming agent comprises the following raw materials by weight: Streptomyces lydicus liquid I 40 parts, lemon grass extract I 30 parts, sodium tripolyphosphate 10 parts, triethanolamine 10 parts, lauroylsodium sodium 30 parts, sodium sulfite 10 parts.

[0052] Preparation method:

[0053] (1) Add triethanolamine, sodium tripolyphosphate, sodium sulfite, lauroylsodium sodium to lemon grass extract I, heat to 30℃, and stir evenly;

[0054] (2) Add Streptomyces lydicus liquid I to the filtrate obtained in step (1), and stir to mix evenly. This is the environmentally friendly plant fiber degumming agent.

[0055] Example 2

[0056] An environmentally friendly plant fiber degumming agent, the environmentally friendly plant fiber degumming agent comprises the following raw materials by weight: Streptomyces lydicus liquid II 40 parts, lemon grass extract I 30 parts, sodium tripolyphosphate 10 parts, triethanolamine 10 parts, lauroylsodium sodium 30 parts, sodium sulfite 10 parts.

[0057] The preparation method is the same as in Example 1.

[0058] Example 3

[0059] An environmentally friendly plant fiber degumming agent, the environmentally friendly plant fiber degumming agent comprises the following raw materials by weight: Streptomyces lydicus liquid II 20 parts, lemon grass extract I 30 parts, sodium tripolyphosphate 10 parts, triethanolamine 10 parts, lauroylsodium sodium 30 parts, sodium sulfite 10 parts.

[0060] The preparation method is the same as that in Embodiment 1.

[0061] Embodiment 4

[0062] An environment-friendly plant fiber degumming agent comprises the following raw materials in parts by weight: Streptomyces lydicus liquid II 20 parts, lemon grass extract II 30 parts, sodium tripolyphosphate 10 parts, triethanolamine 10 parts, sodium lauroyl methylamino acid 30 parts, and sodium sulfite 10 parts.

[0063] The preparation method is the same as that in Embodiment 1.

[0064] Embodiment 5

[0065] An environment-friendly plant fiber degumming agent comprises the following raw materials in parts by weight: Streptomyces lydicus liquid II 20 parts, lemon grass extract II 20 parts, sodium tripolyphosphate 10 parts, triethanolamine 10 parts, sodium lauroyl methylamino acid 30 parts, and sodium sulfite 10 parts.

[0066] The preparation method is the same as that in Embodiment 1.

[0067] Embodiment 6

[0068] An environment-friendly plant fiber degumming agent comprises the following raw materials in parts by weight: Streptomyces lydicus liquid II 20 parts, lemon grass extract II 20 parts, sodium tripolyphosphate 5 parts, triethanolamine 10 parts, sodium lauroyl methylamino acid 30 parts, and sodium sulfite 10 parts.

[0069] The preparation method is the same as that in Embodiment 1.

[0070] Embodiment 7

[0071] An environment-friendly plant fiber degumming agent comprises the following raw materials in parts by weight: Streptomyces lydicus liquid II 20 parts, lemon grass extract II 20 parts, sodium tripolyphosphate 5 parts, triethanolamine 5 parts, sodium lauroyl methylamino acid 30 parts, and sodium sulfite 10 parts.

[0072] The preparation method is the same as that in Embodiment 1.

[0073] Embodiment 8

[0074] An environment-friendly plant fiber degumming agent comprises the following raw materials in parts by weight: Streptomyces lydicus liquid II 20 parts, lemon grass extract II 20 parts, sodium tripolyphosphate 5 parts, triethanolamine 5 parts, sodium lauroyl methylamino acid 20 parts, and sodium sulfite 10 parts.

[0075] The preparation method is the same as that in Embodiment 1.

[0076] Example 9

[0077] An environment-friendly plant fiber degumming agent comprises the following raw materials by weight: Streptomyces lydicus liquid II 20 parts, lemon extract II 20 parts, sodium tripolyphosphate 5 parts, triethanolamine 5 parts, sodium lauroyl methylamino acid 20 parts, and sodium sulfite 5 parts.

[0078] The preparation method is the same as that of Example 1.

[0079] Example 10

[0080] An environment-friendly plant fiber degumming agent comprises the following raw materials by weight: Streptomyces lydicus liquid II 20 parts, lemon extract III 20 parts, sodium tripolyphosphate 5 parts, triethanolamine 5 parts, sodium lauroyl methylamino acid 20 parts, and sodium sulfite 5 parts.

[0081] The preparation method is the same as that of Example 1.

[0082] Comparative Example 1

[0083] The difference from Example 1 is that no Streptomyces lydicus liquid is added.

[0084] Comparative Example 2

[0085] The difference from Example 1 is that no lemon extract is added.

[0086] Comparative Example 3

[0087] The difference from Example 1 is that no Streptomyces lydicus liquid and no lemon extract are added.

[0088] The environment-friendly plant fiber degumming agents prepared in the examples and comparative examples are used in the production of pineapple fibers, and the degumming conditions are as follows: the degumming temperature is 35℃, the pressure is 25MPa, and the degumming time is 30min. The properties of the pineapple fibers produced are tested, the tensile strength and breaking strength are tested according to the national standard GB 7689.5-2001, and the production rate = product mass / total mass of raw materials * 100%, and the specific conditions are shown in Table 1.

[0089] Table 1 Performance test results of environment-friendly plant fiber degumming agents prepared in examples and comparative examples

[0090]

[0091] It can be seen from the comparison of the test results of the comparative example 1 and the example 1 that the environmentally friendly plant fiber degumming agent prepared in the example 1 is added with the streptomyces lydicus bacterial liquid, so that the fiber prepared from the environmentally friendly plant fiber degumming agent has high tensile strength and elongation at break, and high production rate; it can be seen from the comparison of the comparative example 2 and the example 1 that the degumming effect of the degumming agent can be significantly improved by adding the lemon grass extract; it can be seen from the comparison of the example 10 and the example 1 that when the lemon grass extract of the application is not added with the composite enzyme, the degumming effect will be significantly reduced. It can be seen from the comparison of the comparative example 3 and the example 1 that when the environmentally friendly plant fiber degumming agent is not added with the streptomyces lydicus bacterial liquid and the lemon grass extract, the tensile strength, elongation at break, production rate and appearance will be greatly reduced. It can be seen from the comprehensive comparison of the test results of the examples and the comparative examples that the environmentally friendly plant fiber degumming agent provided by the application has excellent degumming effect by using the streptomyces lydicus bacterial liquid for biological catalysis to degrade lignin and make the fiber be resolved out, and the degummed pure dry jute has excellent mechanical properties, high fiber production rate, less by-products, mild process reaction conditions, avoids the use of a large amount of toxic and harmful chemical reagents, thereby reduces the discharge of waste liquid, reduces environmental pollution and wastewater treatment cost, has the advantages of low cost, no pollution to the environment, good degumming effect; by using the lemon grass extract and the composite enzyme in the preparation process, the degumming agent can better adapt to the complex components of gum and specifically degum, degrade the firm lignin in the middle lamella, make the fiber be resolved out, so as to better degrade the gum, maintain the good activity and stability of the streptomyces lydicus bacterial liquid, so that it will not be inactivated in the process of degrading lignin, cooperatively degrade the gum, and has good application prospect in the fiber production.

[0092] The above embodiments are only for illustrating the technical concept and characteristics of the application, and the purpose is to enable those skilled in the art to understand the content of the application and implement it, and cannot limit the protection scope of the application. Any equivalent changes or modifications made according to the spirit and essence of the application should be covered within the protection scope of the application.

Claims

1. An environmentally friendly plant fiber degumming agent, characterized in that, The raw materials include the following weight parts: streptomyces lydicus liquid 20-40 parts, lemon grass extract 20-30 parts, degumming aid 5-10 parts, complexing agent 5-10 parts, surfactant 20-30 parts, inorganic salt 5-10 parts; The preparation method of the streptomyces lydicus liquid comprises the following steps: (1) picking streptomyces lydicus and inoculating into culture medium, and activating on a shaker to obtain activated strain; (2) inoculating the activated strain into liquid culture medium for amplification culture to obtain streptomyces lydicus liquid; The preparation method of the lemon grass extract comprises the following steps: (1) washing lemon grass, drying, crushing, adding distilled water and composite enzyme to obtain powder; (2) distilling the product obtained in step (1), collecting distillate, and separating oil and water to obtain the oil as the lemon grass extract; The mass of the distilled water in step (1) is 3-8 times of that of the lemon grass; the composite enzyme comprises pectinase, ligninase and hemicellulase, and the mass ratio of the pectinase, ligninase and hemicellulase is 1:2-3:2-3, and the mass of the composite enzyme is 10%-15% of that of the lemon grass; The degumming aid is selected from sodium tripolyphosphate.

2. The environment-friendly plant fiber degumming agent according to claim 1, characterized in that, The formula of the culture medium in step (1) is: sucrose 3-5 g / L, glucose 10-20 g / L, soluble starch 1-3 g / L, diammonium hydrogen citrate 1-3 g / L, sodium acetate 5-8 g / L, magnesium sulfate 5-10 g / L, manganese sulfate 2-5 g / L, and agar 20-25 g / L; the formula of the liquid culture medium is: tryptone 5-15 g / L, sodium chloride 8-12 g / L, yeast extract 3-5 g / L, sodium sulfate 3-5 g / L, and deionized water 0.5-1 L; the shaker activation temperature is 30-35℃, and the shaker activation time is 12-16 h; the culture temperature in step (2) is 25-35℃, and the culture time is 48-72 h.

3. The environment-friendly plant fiber degumming agent according to claim 1, characterized in that, The inorganic salt is selected from one or more combinations of sodium sulfite, sodium formate, potassium chloride, sodium chloride and calcium chloride.

4. The environment-friendly plant fiber degumming agent according to claim 1, characterized in that, The complexing agent is selected from one or more combinations of triethanolamine, monoethanolamine and diethanolamine.

5. The environment-friendly plant fiber degumming agent according to claim 1, characterized in that, The surfactant is selected from one or more combinations of sodium lauroyl glutamate, sodium cocoyl glutamate, sodium lauryl glutamate, sodium lauroyl glycinate and sodium cocoyl sarcosinate.

6. A method for preparing the environment-friendly plant fiber degumming agent according to any one of claims 1-5, characterized in that, The preparation method of the environment-friendly plant fiber degumming agent comprises the following steps: (1) adding the complexing agent, degumming aid, inorganic salt and surfactant into the lemon grass extract, heating to 20-30℃, and stirring uniformly; (2) adding the streptomyces lydicus liquid into the filtrate obtained in step (1) and stirring uniformly to obtain the environment-friendly plant fiber degumming agent.

7. Use of the environmentally friendly plant fiber degumming agent according to any one of claims 1-5 in a plant fiber degumming process, characterized in that, The application conditions of the environment-friendly plant fiber degumming agent in the degumming process are: degumming temperature 30-40℃, pressure 20-25 MPa, and degumming time 20-30 min.

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