Electrochemical flexible sensor for simultaneous detection of glucose and ethanol and detection method

Abstract

This invention discloses an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol. It involves introducing a zinc oxide nanowire array and palladium nanoparticles onto the surface of carbon cloth to obtain a CC / ZnO / Pd modified electrode. A three-electrode system is then formed, using the CC / ZnO / Pd modified electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode. This system can simultaneously detect the glucose and ethanol content of a sample, with detection ranges of 1×10⁻⁶ for glucose and ethanol, respectively. ‑6 -1×10 ‑1 mol / L and 1×10 ‑7 -1×10 ‑2 The detection limits were 5.20 × 10 mol / L. ‑7 mol / L ( S / N =3), 1.17×10 ‑8 mol / L ( S / N =3). It is not only simple to operate, efficient, and low in cost, but also has a lower detection limit and a wider linear range, which can accurately distinguish between alcoholic hypoglycemia and intoxication, avoiding misdiagnosis and mistreatment.

Description

Technical Field

[0001] This invention relates to an electrochemical flexible sensor and detection method, and more particularly to an electrochemical flexible sensor and detection method capable of simultaneously detecting glucose and ethanol. Background Technology

[0002] Glucose (Glu), chemical formula C6H 12 Oxygen (O6) is an essential nutrient for metabolism in living organisms and a necessary energy source for human life activities. If the level of glucose in the blood is not properly regulated, diseases such as diabetes and hypoglycemia can occur. Hypoglycemia syndrome caused by alcohol poisoning is called alcoholic hypoglycemia, which is often accompanied by symptoms of glucose deficiency in the central nervous system. It is not easily distinguished from a state of drunkenness and often leads to misdiagnosis and mistreatment.

[0003] Currently, the GOD-POD method is commonly used for serum glucose determination. Glucose oxidase (GOD) uses oxygen to oxidize glucose to gluconic acid, releasing hydrogen peroxide in the process. Peroxidase (POD) catalyzes the oxidation of chromogens by peroxide, and then oxidizes the chromogens to pigments, i.e., the Trinder reaction. The amount of red quinone compounds produced is directly proportional to the glucose content, and blood glucose concentration can be measured using spectrophotometry. Common methods for ethanol detection include chromatography, spectrophotometry, colorimetry, and electrochemical methods. Among these, chromatography and spectrophotometry have disadvantages such as long detection times and difficulty in on-site operation, while colorimetric results are easily affected by volatile alcohols. Electrochemical methods, on the other hand, are receiving increasing attention due to their wide linear range and low detection limit.

[0004] To date, there have been no reports on electrochemical sensors that can simultaneously detect glucose and ethanol. As a result, to accurately distinguish between alcoholic hypoglycemia and drunkenness, it is necessary to detect the glucose and ethanol levels in the blood separately, which is not only complicated, inefficient, and costly, but also causes corresponding physical and mental suffering to the test subjects. Summary of the Invention

[0005] The present invention aims to solve the above-mentioned technical problems existing in the prior art by providing an electrochemical flexible sensor and detection method that can simultaneously detect glucose and ethanol.

[0006] The technical solution of this invention is: an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode. The reference electrode is a saturated calomel electrode, and the counter electrode is a platinum wire. The working electrode is a CC / ZnO / Pd modified electrode, prepared sequentially according to the following steps:

[0007] Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise to 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain mixed solution A. Mixed solution A is hydrothermally heated at 60℃ for 2-4 h to obtain ZnO nanoparticle colloidal solution. The volume ratio of sodium hydroxide anhydrous ethanol solution to zinc acetate dihydrate anhydrous ethanol solution is 1:1.

[0008] Step 2. Repeat the immersion-drying process of carbon cloth, wherein the immersion-drying process involves immersing the carbon cloth in a ZnO nanoparticle colloidal solution for at least 30 min, and then drying it in a 95 ℃ oven for 30 min to obtain impregnated carbon cloth;

[0009] Step 3. Under uniform stirring, add 0.75 mol / L NaOH aqueous solution dropwise to 0.035 mol / L Zn(CH3COO)2·2H2O aqueous solution, and continue stirring for 15-30 min to obtain mixed solution B. The volume ratio of NaOH aqueous solution to Zn(CH3COO)2·2H2O aqueous solution is 1:1.

[0010] Step 4. Place the mixed solution B and the impregnated carbon cloth in the reaction vessel, and then react the reaction vessel at 70°C for 12-16 hours. Cool it at room temperature overnight, take out the impregnated carbon cloth, rinse it alternately with water and ethanol, and dry it at 60°C to obtain the CC / ZnO electrode.

[0011] Step 5. Place the three-electrode system—with a CC / ZnO electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode—in a 0.1 mol / L PdCl2 aqueous solution. The system is then tested at a potential of -1.0 V and a sensitivity of 1 × 10⁻⁶. -2 Electrodeposition under A / V conditions for 300-500 s yields a CC / ZnO / Pd modified electrode.

[0012] A detection method for the aforementioned electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol is characterized by: a potential range of -0.4 to 0.9 V, a potential increment of 4 mV, an amplitude of 50 mV, and a sensitivity of 1 × 10⁻⁶. -3 Under A / V conditions, for a substance containing 1×10 -6 -1×10 -1 mol / L glucose and 1×10 -7 -1×10 -2 The sample was tested for mol / L ethanol.

[0013] This invention introduces a zinc oxide nanowire array and palladium nanoparticles onto the surface of carbon cloth to obtain a CC / ZnO / Pd modified electrode. A three-electrode system is then formed using the CC / ZnO / Pd modified electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode. This system can simultaneously detect the glucose and ethanol content of a sample, with detection ranges of 1×10⁻⁶ for glucose and ethanol, respectively. -6 -1×10 -1 mol / L and 1×10 -7 -1×10 -2 The detection limits were 5.20 × 10 mol / L. -7 mol / L ( S / N =3), 1.17×10 -8 mol / L ( S / N =3). Compared with existing technologies, it is not only simple to operate, efficient, and low in cost, but also has a lower detection limit and a wider linear range, which can accurately distinguish between alcoholic hypoglycemia and intoxication, avoiding misdiagnosis and mistreatment. Attached Figure Description

[0014] Figure 1 These are scanning electron microscope images of different modified electrodes from Embodiment 1 of the invention.

[0015] Figure 2 These are the CV curves of different modified electrodes in Embodiment 1 of the invention.

[0016] Figure 3 This is the DPV curve of Embodiment 1 of the invention.

[0017] Figure 4 This is the working curve of the CC / ZnO / Pd modified electrode in Embodiment 1 of the invention. Detailed Implementation Example 1

[0018] The present invention discloses an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode. The reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire, and the working electrode is a CC / ZnO / Pd modified electrode, which is prepared sequentially according to the following steps:

[0019] Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise to 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain mixed solution A. Mixed solution A is hydrothermally heated at 60℃ for 2-4 h to obtain ZnO nanoparticle colloidal solution. The volume ratio of sodium hydroxide anhydrous ethanol solution to zinc acetate dihydrate anhydrous ethanol solution is 1:1.

[0020] Step 2. Repeat the immersion-drying process three times with the carbon cloth (CC). The immersion-drying process involves immersing the carbon cloth in a ZnO nanoparticle colloidal solution for at least 30 min, and then drying it in a 95 ℃ oven for 30 min to obtain impregnated carbon cloth.

[0021] Step 3. Under uniform stirring, add 0.75 mol / L NaOH aqueous solution dropwise to 0.035 mol / L Zn(CH3COO)2·2H2O aqueous solution, and continue stirring for 15 min to obtain mixed solution B. The volume ratio of NaOH aqueous solution to Zn(CH3COO)2·2H2O aqueous solution is 1:1.

[0022] Step 4. Place the mixed solution B and the impregnated carbon cloth in a polytetrafluoroethylene-lined reactor, then place the reactor in an electric heating drying oven at 70°C for 12 hours. Remove the reactor and cool it at room temperature overnight. Then remove the impregnated carbon cloth from the reactor, rinse it three times with water and ethanol alternately, and dry it at 60°C to obtain the CC / ZnO electrode.

[0023] Step 5. Place the three-electrode system—with a CC / ZnO electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode—in a 0.1 mol / L PdCl2 aqueous solution. The system is then tested at a potential of -1.0 V and a sensitivity of 1 × 10⁻⁶. -2 Electrodeposition under A / V conditions for 300 s yielded a CC / ZnO / Pd modified electrode.

[0024] The detection method in Embodiment 1 of this invention operates within a potential range of -0.4 to 0.9 V, with a potential increment of 4 mV, an amplitude of 50 mV, and a sensitivity of 1 × 10⁻⁶. -3 Under A / V conditions, for a substance containing 1×10 -6 -1×10 -1 mol / L glucose and 1×10 -7 -1×10 -2 The sample was tested for mol / L ethanol.

[0025] Scanning electron microscope images of different electrodes in Embodiment 1 of the present invention are as follows: Figure 1 As shown. Figure 1 In the image, A represents a carbon cloth / zinc oxide electrode; B represents a carbon cloth / zinc oxide / nano palladium electrode. From... Figure 1 It can be seen that after modifying zinc oxide, a zinc oxide nanowire array resembling small grasses is formed on the surface of the carbon cloth electrode; after depositing palladium nanowires, it can be seen that the palladium nanowires on the CC / ZnO / Pd modified electrode surface are effectively deposited on the modified electrode, and the palladium nanowires are evenly distributed on the surface of the zinc oxide nanowires, resembling corn kernels.

[0026] Different electrodes in Example 1 of this invention were used in a solution of 0.1 mol / L KCl + 5 mmol / L [Fe(CN)6] 3- / 4 The CV curve in the electrolyte solution (0.1 mol / L PBS, pH=7.0) is shown below. Figure 2 As shown. Figure 2 In the figure, curve 1 shows the CV results for the carbon cloth electrode. It can be seen that I... pa / I pc ≈1, exhibiting a reversible redox reaction, is [Fe(CN)6]. 3- / 4- Characteristic electrochemical behavior on carbon cloth electrodes; Curve 2 shows the CV results of the CC / ZnO modified electrode. The peak current of Curve 2 is lower than that of Curve 1. This may be because zinc oxide nanowire arrays were successfully hydrothermally synthesized on the carbon cloth surface, resulting in a lower electron transport rate. Curve 3 shows the CV results of the CC / ZnO / Pd modified electrode. The peak current value of this electrode is much larger than that of Curve 1 and Curve 2, indicating a higher electron transport rate. This is because the conductivity is greatly enhanced by modifying the zinc oxide nanowire surface with palladium nanoparticles.

[0027] The DPV curves of the electrochemical biosensor in Example 1 of this invention, which directly detects different concentrations of Glu and Eth, are shown below. Figure 3 As shown. Figure 3 (A) shows the DPV curves for detecting different concentrations of ethanol. The peak position of Eth is -0.084 V, and the peak current of CC / ZnO / Pd gradually increases with increasing Eth concentration. (B) shows the DPV curves for detecting different concentrations of glucose. The peak position of Glu is 0.57 V, and the peak current of CC / ZnO / Pd gradually increases with increasing Glu concentration. (C) shows the DPV curves for detecting mixed solutions of ethanol and glucose at different concentrations. It can be seen that the peak current gradually increases with increasing mixed solution concentration. Figure 3 Comparative analysis of A, B, and C shows that the peak current position of DPV with dual-substance detection is consistent with the peak current position of Glu or Eth detected alone, indicating that the CC / ZnO / Pd modified electrode can detect Glu and Eth simultaneously.

[0028] Based on the relationship between the DPV peak current value and the logarithm of the mixed solution concentration, a standard curve analysis was performed, and the working curve is shown below. Figure 4 As shown. Figure 4 A shows the working curve for detecting Glu: the linear range for Glu detection using the CC / ZnO / Pd modified electrode is 1×10⁻⁶. -6 -1×10 -1 mol / L, the calculated linear regression equation is as follows: I (μA) = 6.65 logC (mol / L) + 99.026, the linear correlation coefficient is 0.996, and the detection limit is 5.20 × 10⁻⁶.-7 mol / L ( S / N =3); Figure 4 B shows the working curve for Eth detection using a CC / ZnO / Pd modified electrode: the linear range of Eth is 1×10⁻⁶. -7 -1×10 -2 mol / L, the calculated linear regression equation is as follows: I (μA) = 11.65 logC (mol / L) + 233.68, correlation coefficient is 0.994, detection limit is 1.17 × 10⁻⁶ -8 mol / L ( S / N =3). Example 2

[0029] The present invention discloses an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode. The reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire, and the working electrode is a CC / ZnO / Pd modified electrode, which is prepared sequentially according to the following steps:

[0030] Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise to 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain mixed solution A. Mixed solution A is hydrothermally heated at 60℃ for 2-4 h to obtain ZnO nanoparticle colloidal solution. The volume ratio of sodium hydroxide anhydrous ethanol solution to zinc acetate dihydrate anhydrous ethanol solution is 1:1.

[0031] Step 2. Repeat the immersion-drying process three times with the carbon cloth (CC). The immersion-drying process involves immersing the carbon cloth in a ZnO nanoparticle colloidal solution for at least 30 min, and then drying it in a 95 ℃ oven for 30 min to obtain impregnated carbon cloth.

[0032] Step 3. Under uniform stirring, add 0.75 mol / L NaOH aqueous solution dropwise to 0.035 mol / L Zn(CH3COO)2·2H2O aqueous solution, and continue stirring for 30 min to obtain mixed solution B. The volume ratio of NaOH aqueous solution to Zn(CH3COO)2·2H2O aqueous solution is 1:1.

[0033] Step 4. Place the mixed solution B and the impregnated carbon cloth in a polytetrafluoroethylene-lined reactor, then place the reactor in an electric heating drying oven at 70°C for 16 hours. Remove the reactor and cool it at room temperature overnight. Then remove the impregnated carbon cloth from the reactor, rinse it three times with water and ethanol alternately, and dry it at 60°C to obtain the CC / ZnO electrode.

[0034] Step 5. Place the three-electrode system—with a CC / ZnO electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode—in a 0.1 mol / L PdCl2 aqueous solution. The system is then tested at a potential of -1.0 V and a sensitivity of 1 × 10⁻⁶. -2 Electrodeposition under A / V conditions for 300 s yielded a CC / ZnO / Pd modified electrode.

[0035] The detection method is the same as in Example 1. Example 3

[0036] The present invention discloses an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode. The reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire, and the working electrode is a CC / ZnO / Pd modified electrode, which is prepared sequentially according to the following steps:

[0037] Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise to 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain mixed solution A. Mixed solution A is hydrothermally heated at 60℃ for 2-4 h to obtain ZnO nanoparticle colloidal solution. The volume ratio of sodium hydroxide anhydrous ethanol solution to zinc acetate dihydrate anhydrous ethanol solution is 1:1.

[0038] Step 2. Repeat the immersion-drying process three times with the carbon cloth (CC). The immersion-drying process involves immersing the carbon cloth in a ZnO nanoparticle colloidal solution for at least 30 min, and then drying it in a 95 ℃ oven for 30 min to obtain impregnated carbon cloth.

[0039] Step 3. Under uniform stirring, add 0.75 mol / L NaOH aqueous solution dropwise to 0.035 mol / L Zn(CH3COO)2·2H2O aqueous solution, and continue stirring for 25 min to obtain mixed solution B. The volume ratio of NaOH aqueous solution to Zn(CH3COO)2·2H2O aqueous solution is 1:1.

[0040] Step 4. Place the mixed solution B and the impregnated carbon cloth in a polytetrafluoroethylene-lined reactor, then place the reactor in an electric heating drying oven at 70°C for 14 hours. Remove the reactor and cool it at room temperature overnight. Then remove the impregnated carbon cloth from the reactor, rinse it three times with water and ethanol alternately, and dry it at 60°C to obtain the CC / ZnO electrode.

[0041] Step 5. Place the three-electrode system—with a CC / ZnO electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode—in a 0.1 mol / L PdCl2 aqueous solution. The system is then tested at a potential of -1.0 V and a sensitivity of 1 × 10⁻⁶.-2 Electrodeposition under A / V conditions for 300 s yielded a CC / ZnO / Pd modified electrode.

[0042] The detection method is the same as in Example 1. Example 4

[0043] The present invention discloses an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode. The reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire, and the working electrode is a CC / ZnO / Pd modified electrode, which is prepared sequentially according to the following steps:

[0044] Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise to 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain mixed solution A. Mixed solution A is hydrothermally heated at 60℃ for 2-4 h to obtain ZnO nanoparticle colloidal solution. The volume ratio of sodium hydroxide anhydrous ethanol solution to zinc acetate dihydrate anhydrous ethanol solution is 1:1.

[0045] Step 2. Repeat the immersion-drying process three times with the carbon cloth (CC). The immersion-drying process involves immersing the carbon cloth in a ZnO nanoparticle colloidal solution for at least 30 min, and then drying it in a 95 ℃ oven for 30 min to obtain impregnated carbon cloth.

[0046] Step 3. Under uniform stirring, add 0.75 mol / L NaOH aqueous solution dropwise to 0.035 mol / L Zn(CH3COO)2·2H2O aqueous solution, and continue stirring for 15 min to obtain mixed solution B. The volume ratio of NaOH aqueous solution to Zn(CH3COO)2·2H2O aqueous solution is 1:1.

[0047] Step 4. Place the mixed solution B and the impregnated carbon cloth in a polytetrafluoroethylene-lined reactor, then place the reactor in an electric heating drying oven at 70°C for 15 hours. Remove the reactor and cool it at room temperature overnight. Then remove the impregnated carbon cloth from the reactor, rinse it three times with water and ethanol alternately, and dry it at 60°C to obtain the CC / ZnO electrode.

[0048] Step 5. Place the three-electrode system—with a CC / ZnO electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode—in a 0.1 mol / L PdCl2 aqueous solution. The system is then tested at a potential of -1.0 V and a sensitivity of 1 × 10⁻⁶. -2 Electrodeposition under A / V conditions for 300 s yielded a CC / ZnO / Pd modified electrode.

[0049] The detection method is the same as in Example 1. Example 5

[0050] The present invention discloses an electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode. The reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire, and the working electrode is a CC / ZnO / Pd modified electrode, which is prepared sequentially according to the following steps:

[0051] Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise to 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain mixed solution A. Mixed solution A is hydrothermally heated at 60℃ for 2-4 h to obtain ZnO nanoparticle colloidal solution. The volume ratio of sodium hydroxide anhydrous ethanol solution to zinc acetate dihydrate anhydrous ethanol solution is 1:1.

[0052] Step 2. Repeat the immersion-drying process three times with the carbon cloth (CC). The immersion-drying process involves immersing the carbon cloth in a ZnO nanoparticle colloidal solution for at least 30 min, and then drying it in a 95 ℃ oven for 30 min to obtain impregnated carbon cloth.

[0053] Step 3. Under uniform stirring, add 0.75 mol / L NaOH aqueous solution dropwise to 0.035 mol / L Zn(CH3COO)2·2H2O aqueous solution, and continue stirring for 30 min to obtain mixed solution B. The volume ratio of NaOH aqueous solution to Zn(CH3COO)2·2H2O aqueous solution is 1:1.

[0054] Step 4. Place the mixed solution B and the impregnated carbon cloth in a polytetrafluoroethylene-lined reactor, then place the reactor in an electric heating drying oven at 70°C for 14 hours. Remove the reactor and cool it at room temperature overnight. Then remove the impregnated carbon cloth from the reactor, rinse it three times with water and ethanol alternately, and dry it at 60°C to obtain the CC / ZnO electrode.

[0055] Step 5. Place the three-electrode system—with a CC / ZnO electrode as the working electrode, a saturated calomel electrode as the reference electrode, and a platinum wire as the counter electrode—in a 0.1 mol / L PdCl2 aqueous solution. The system is then tested at a potential of -1.0 V and a sensitivity of 1 × 10⁻⁶. -2 Electrodeposition under A / V conditions for 300 s yielded a CC / ZnO / Pd modified electrode.

[0056] The detection method is the same as in Example 1.

Claims

1. An electrochemical flexible sensor capable of simultaneously detecting glucose and ethanol, comprising a three-electrode system consisting of a working electrode, a reference electrode, and a counter electrode, wherein the reference electrode is a saturated calomel electrode and the counter electrode is a platinum wire, characterized in that: The working electrode is a CC / ZnO / Pd modified electrode, which is prepared according to the following steps in sequence: Step 1. Under continuous stirring, 4 mmol / L sodium hydroxide anhydrous ethanol solution is added dropwise into 4 mmol / L zinc acetate dihydrate anhydrous ethanol solution to obtain a mixed solution A, and the mixed solution A is hydrothermally treated at 60 DEG C for 2-4 h to obtain a ZnO nanoparticle colloidal solution, wherein the volume ratio of the sodium hydroxide anhydrous ethanol solution to the zinc acetate dihydrate anhydrous ethanol solution is 1:1; Step 2. The carbon cloth is repeatedly immersed and dried, wherein the immersion and drying is that the carbon cloth is immersed in the ZnO nanoparticle colloidal solution for at least 30 min, and then dried in a 95 DEG C oven for 30 min to obtain an impregnated carbon cloth; Step 3. Under uniform stirring, 0.75 mol / L NaOH aqueous solution is added dropwise into 0.035 mol / L Zn(CH3COO)2.2H2O aqueous solution, and the stirring is continuously carried out for 15-30 min to obtain a mixed solution B, wherein the volume ratio of the NaOH aqueous solution to the Zn(CH3COO)2.2H2O aqueous solution is 1:1; Step 4. The mixed solution B and the impregnated carbon cloth are placed in a reaction kettle, and the reaction kettle is reacted at 70 DEG C for 12-16 h, and then cooled at room temperature overnight, and the impregnated carbon cloth is taken out, washed with water and ethanol alternately, and dried at 60 DEG C to obtain a CC / ZnO electrode; Step 5. The three-electrode system of CC / ZnO electrode as working electrode, saturated calomel electrode as reference electrode and platinum wire as counter electrode was placed in 0.1 mol / L PdCl2 aqueous solution, and the electrodeposition was carried out at a potential of -1.0 V and a sensitivity of 1 x 10 -2 A / V for 300-500 s to obtain a CC / ZnO / Pd modified electrode.

2. A method for detecting the electrochemical flexible sensor for simultaneously detecting glucose and ethanol according to claim 1, characterized in that: In the potential range of -0.4-0.9 V, the potential increment is 4 mV, the amplitude is 50 mV, the sensitivity is 1×10 -3 A / V conditions, the sample containing 1×10 -6 -1×10 -1 mol / L glucose and 1×10 -7 -1×10 -2 mol / L ethanol is detected.

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