A Quantitative Analysis Method for Sotolon in Chinese Yellow Rice Wine

Abstract

The present invention discloses a quantitative analysis method for sotolon in yellow rice wine, belonging to the technical field of chemical detection. The quantitative analysis method for sotolon in yellow rice wine according to the present invention first enriches and concentrates sotolon in the yellow rice wine sample through extraction and concentration treatment with a specific extractant, then quickly purifies the sample by thin-layer chromatography to remove most of the impurity interferences, and finally accurately quantifies sotolon by UHPLC-DAD. The test results have little interference and high accuracy. The operation steps are simple and efficient, the implementation cost is low, and the economic cost performance is high. It can be used for the quality control of a large number of yellow rice wine products in industrial production.

Description

Technical Field

[0001] The present invention relates to the technical field of chemical detection, and particularly relates to a method for quantitative analysis of sotolon in Chinese rice wine. Background Art

[0002] Sotolon is an important aroma substance in wines such as wine, Chinese rice wine, and sake, and can be used as an important quality control index for traditional fermented wines. However, sotolon has the characteristics of strong polarity and poor thermal stability, has a very low content in wines, and has a very poor signal response in mass spectrometry detection. Commonly used methods for pretreatment and quantitative detection of aroma substances, such as SPME, SBSE, and Head space combined with GC-MS analysis methods, are all very difficult to accurately quantify sotolon.

[0003] Currently, the main detection methods for sotolon are as follows: (1) The determination of trace sotolon is realized based on solid-phase extraction technology and large-volume injection technology. This technical solution selects special adsorbents such as LiChrolut EN to adsorb and extract sotolon in the sample, and then uses the microtube large-volume injection technology to increase the injection volume, and finally increases the signal response of mass spectrometry to sotolon (for example, "Research on the Volatile Components and Aroma Characteristics of Chinese Rice Wine" - Chen Shuang). However, this method requires unconventional equipment, has a high detection cost, and is not easy to promote. (2) Liquid-liquid microextraction combined with GC-MS is used for quantitative analysis of sotolon. This technical solution can quickly process a large number of samples through testing (for example, "Research on the Key Aroma Components and Their Formation Influencing Factors during the Aging of Chinese Rice Wine" - Wang Chengcheng). However, the components in Chinese rice wine are very complex, and there are many interfering substances when directly injecting the sample for detection after extraction, and there is also the problem of low mass spectrometry response of sotolon, resulting in insufficient accuracy of the detection results. (3) Stir bar sorptive extraction combined with GC-MS is used to analyze the aroma composition of Chinese rice wine (for example, "Determination of Volatile Aroma Substances in Aged Chinese Rice Wine by Gas Chromatography-Mass Spectrometry Combined with Adsorptive Extraction" - Yu Haiyan). However, this technical solution has no specificity for sotolon and has a large error for low-concentration samples. Summary of the Invention

[0004] Based on the defects existing in the prior art, the purpose of the present invention is to provide a method for quantitative analysis of sotolon in Chinese rice wine. This method enriches and concentrates sotolon in the Chinese rice wine sample through extraction and concentration treatment with a specific extractant, then uses thin-layer chromatography to quickly purify the sample to remove most of the impurity interferences, and finally uses UHPLC-DAD for accurate quantitative analysis of sotolon. The test results have little interference, high accuracy rate, the operation steps are simple and efficient, the implementation cost is low, the economic cost performance is high, and it can be used for the quality control of large batches of Chinese rice wine products in factories.

[0005] In order to achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] A quantitative analysis method for sotolon in yellow rice wine, comprising the following steps:

[0007] (1) Take a yellow rice wine sample and extract it with an extractant, and then obtain concentrated solution I after concentration treatment; the extractant is dichloromethane, and the volume ratio of the yellow rice wine sample to dichloromethane is 1:(0.15 - 0.25);

[0008] (2) Perform thin-layer chromatography on concentrated solution I to obtain chromatographic treatment product II; the developing agent for the thin-layer chromatography treatment is prepared from diethyl ether and petroleum ether 30 - 60 according to the volume ratio (0.8 - 1.2):(0.8 - 1.2);

[0009] (3) Perform UHPLC-DAD detection on chromatographic treatment product II and the sotolon standard solution to quantitatively analyze sotolon in the yellow rice wine sample.

[0010] Preferably, the yellow rice wine sample includes yuanhong wine sample, jiafan wine sample, shanliang wine sample and xiangxue wine sample.

[0011] In the quantitative analysis method for sotolon in yellow rice wine of the present invention, an extractant with strong solubility and low toxicity, dichloromethane, is used to effectively extract and separate sotolon according to a specific addition ratio; then after concentration treatment, compared with the prior art, the technical solution of the present invention uses a developing agent to perform secondary purification on the concentrated solution sample. This step can effectively remove most of the organic phase interfering impurities in the sample on the premise of simple operation; at the same time, after screening, the mixed developing agent prepared from diethyl ether and petroleum ether 30 - 60 according to a specific ratio has higher specific separation for sotolon compared with other types of selections. Finally, the treated sample is detected and analyzed by UHPLC-DAD (ultra-high performance liquid chromatography coupled with ultraviolet detection), and the content of trace-level sotolon in various yellow rice wines including yuanhong wine, jiafan wine, shanliang wine, xiangxue wine, etc. can be effectively determined, which is simple and efficient.

[0012] Preferably, before the extraction of the yellow rice wine sample with the extractant in step (1), sodium chloride is further added, and the addition amount of sodium chloride accounts for 4 - 6% of the mass content of the yellow rice wine sample.

[0013] By introducing an appropriate amount of sodium chloride before the organic extraction of the yellow rice wine sample, the solubility of the effective substance in the aqueous phase can be effectively reduced, and the extraction rate of the extractant can be improved.

[0014] Preferably, the chromatographic plate used for the thin-layer chromatography treatment in step (2) is a GF254 silica gel thin-layer chromatographic plate.

[0015] The GF254 silica gel thin layer chromatography plate is a commonly used identification / separation chromatography plate. When performing chromatography on the concentrated solution I after concentration treatment as a fluorescence-sensitive side-suction plate, the purification degree of trigonelline can be observed through ultraviolet fluorescence. At the same time, the sample after chromatography treatment can be directly cut to separate the corresponding silica gel part. Those skilled in the art can also choose to use a complex solvent to re-dissolve the sample according to the actual situation.

[0016] Preferably, the chromatographic column used for UHPLC-DAD detection in step (3) is an Eclipse Plus C18 series chromatographic column. The specifications of the chromatographic column during UHPLC-DAD detection are 1.8μm 2.1mm×150mm, and the column temperature of the chromatographic column is 30 - 40°C.

[0017] More preferably, during UHPLC-DAD detection, the mobile phase A for elution is an aqueous solution of trifluoroacetic acid with a mass content of 0.08 - 0.12 wt%, and the mobile phase B is methanol. The specific elution procedure is as follows: the injection volume is 1.8 - 2.2 μL, from 0 to 10 min, the volume ratio of mobile phase A to mobile phase B is 85:15, and the flow rate of the mobile phase is 0.28 - 0.32 mL / min. More preferably, the DAD detection wavelength during UHPLC-DAD detection in step (3) is 200 - 250 nm.

[0018] When UHPLC-DAD detection is applied to the quantitative detection of trigonelline in yellow rice wine with different concentrations, it has the advantages of high separation efficiency and accurate test results. Since the chromatographic column packing used in this technical solution has a small particle size, short time consumption, a large amount of detector detection information, and a concentrated analysis range, the elution and detection conditions of the sample to be tested need to be specific. After screening, the detection set under the above conditions in combination with the previous purification treatment is suitable for the high-efficiency quantitative analysis of trigonelline in various yellow rice wine products.

[0019] Preferably, the UHPLC-DAD detection in step (3) also includes the drawing of the standard curve of trigonelline. The specific steps are as follows: Prepare working solutions of trigonelline with concentrations of 1 mg / mL, 5 mg / mL, 10 mg / mL, 40 mg / mL, and 100 mg / mL by diluting the standard solution of trigonelline, and perform UHPLC-DAD detection and draw the standard curve.

[0020] Another object of the present invention is to provide the application of the quantitative analysis method of trigonelline in yellow rice wine in quality control during the batch production process of yellow rice wine.

[0021] Due to the large number of volatile substances in yellow rice wine, and during the production process before sealed storage, these substances are very prone to volatilization or deterioration, directly affecting the quality of yellow rice wine products. Therefore, quality control during the production of yellow rice wine, especially in batch production, is very important. The quantitative analysis method of trigonelline in the yellow rice wine described in the present invention has high analysis efficiency, high sensitivity for the active substance trigonelline in yellow rice wine, short time consumption, does not rely on special equipment, is convenient to carry out, and is especially suitable for quality control during the production process of batch yellow rice wine.

[0022] The beneficial effect of the present invention is that the present invention provides a quantitative analysis method of trigonelline in yellow rice wine. This method first enriches and concentrates the trigonelline in the yellow rice wine sample through extraction and concentration treatment with a specific extractant, and then uses thin-layer chromatography to quickly purify the sample to remove most of the impurity interferences. Finally, UHPLC-DAD is used for accurate quantitative analysis of trigonelline. The test results have little interference, the characteristic peak emergence time is short, and the accuracy is high. The operation steps are simple and efficient, the implementation cost is low, and the economic cost performance is high. It can be used for quality control of factory-produced large quantities of yellow rice wine products. Brief Description of the Drawings

[0023] Figure 1 It is the standard curve of the trigonelline standard sample in Example 1 of the present invention;

[0024] Figure 2 It is the DAD chromatogram of the trigonelline standard sample in Example 1 of the present invention;

[0025] Figure 3 It is the chromatogram after thin-layer chromatography treatment of the sample using the developing agent described in Example 1 in Comparative Example 1 of the present invention;

[0026] Figure 4 It is the chromatogram after thin-layer chromatography treatment of the sample using the developing agent described in Control Group 1 in Comparative Example 1 of the present invention;

[0027] Figure 5 It is the chromatogram after thin-layer chromatography treatment of the sample using the developing agent described in Control Group 2 in Comparative Example 1 of the present invention;

[0028] Figure 6 It is the chromatogram after thin-layer chromatography treatment of the sample using the developing agent described in Control Group 3 in Comparative Example 1 of the present invention;

[0029] Figure 7 It is the chromatogram after thin-layer chromatography treatment of the sample using the developing agent described in Control Group 4 in Comparative Example 1 of the present invention;

[0030] Figure 8 It is the chromatogram after thin-layer chromatography treatment of the sample using the developing agent described in Control Group 3 in Comparative Example 1 of the present invention;

[0031] Figure 9 This is the DAD chromatogram of the chromatographically processed product obtained by using the quantitative analysis method described in Example 1 in Comparative Example 2 of the present invention;

[0032] Figure 10 This is the DAD chromatogram of the chromatographically processed product obtained by using the quantitative analysis method described in Control Group 6 in Comparative Example 2 of the present invention. Detailed implementation manners

[0033] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific examples and comparative examples. The purpose is to understand the content of the present invention in detail, rather than a limitation to the present invention. All other examples obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The experimental reagents, raw materials and instruments involved in the examples and comparative examples of the present invention are all common ordinary reagents, raw materials and instruments unless otherwise specified. The trigonelline used for preparing the standard solution of trigonelline in the present invention is purchased from Sigma-Aldrich (Shanghai, China) Co., Ltd.; the GF254 silica gel thin layer chromatography plate is purchased from Qingdao Ocean Chemical Co., Ltd.

[0034] Example 1

[0035] An example of the quantitative analysis method of trigonelline in the yellow rice wine described in the present invention includes the following steps:

[0036] (1) Transfer 100 mL of commercially available yellow rice wine sample Z into a 500 mL separatory funnel, add sodium chloride with a mass ratio of 5 wt% of the sample, mix well, extract twice with 20 mL of dichloromethane each time. After vortex oscillation for 30 s, wait for the mixed solution to stand and separate, collect the lower organic phase and combine them, and concentrate quantitatively to 100 μL by nitrogen blowing to obtain concentrated solution I;

[0037] (2) Use a capillary to quantitatively spot 20 μL of concentrated solution I on a GF254 silica gel thin-layer chromatography plate. Synchronously spot the same amount of 1000 mg / mL trigonelline lactone standard solution on the plate. Use a mixed solution prepared by mixing diethyl ether and petroleum ether 30 - 60 in a volume ratio of 1:1 as the developing agent for chromatographic separation. After the separation is completed, observe and confirm under fluorescence. Subsequently, directly cut the silica gel at the same chromatographic distance as the standard solution to separate the active substance trigonelline lactone. Redissolve the trigonelline lactone in the cut silica gel with 0.25 mL of an aqueous solution of methanol with a volume content of 50%. After sucking out the redissolved liquid with a pipette, repeat the same operation, and combine the sucked liquid with the first redissolved liquid. After passing through a 0.22 μm organic membrane, obtain the chromatographically treated product II; (3) Perform UHPLC-DAD detection on the chromatographically treated product II and the trigonelline lactone standard solution, and conduct quantitative analysis of trigonelline lactone in the yellow rice wine sample. The test instrument used for the UHPLC-DAD detection is an ultra-high performance liquid chromatograph Agilent 1290 Infinity, equipped with a DAD detector with a detection wavelength of 235 nm; The specific test conditions are as follows:

[0038] Time (min) A(％) B(％) 0 85 15 10 85 15

[0039] Among them, an aqueous solution of trifluoroacetic acid with a mass content of 0.1 wt% is used as mobile phase A, and methanol is used as mobile phase B. The flow rate of the mobile phase is 0.3 mL / min;

[0040] The specific steps are as follows:

[0041] (1) Draw a standard curve of trigonelline lactone: Prepare an initial standard solution of trigonelline lactone at 1000 mg / L using the standard product trigonelline lactone. Subsequently, gradually dilute and prepare working solutions of trigonelline lactone with concentrations of 1 mg / mL, 5 mg / mL, 10 mg / mL, 40 mg / mL, and 100 mg / mL respectively. Perform UHPLC-DAD detection and draw the standard curve; The results of the standard curve drawing are as Figure 1 shown, and the DAD chromatogram of the standard product is as Figure 2 shown. The parameters of this standard curve are shown in Table 1; It can be seen from Figure 2 that the peak emergence time of trigonelline lactone in the quantitative analysis method of the present invention is very short, and the corresponding characteristic peak can appear in the chromatogram in only about 5 min.

[0042] Table 1

[0043]

[0044] (2) Perform UHPLC-DAD detection on the chromatographic processed product B to determine the content concentration of sotolon in the original yellow rice wine sample Z (spiked substrate). Conduct parallel tests three times, and the results are shown in Table 2.

[0045] Table 2

[0046]

[0047] As can be seen from Table 2, the accuracy of parallel tests for the same sample is high, and the RSD% is within 5%.

[0048] Subsequently, using the yellow rice wine sample Z as the substrate, add a certain amount of sotolon to it. The obtained mixture is processed and detected according to the above treatment method, and the results are shown in Table 3; where the recovery rate % = (C2 - C0) / C1 × 100%, C0 is the concentration of sotolon in the substrate before adding the additional sotolon, C1 is the concentration of the added sotolon, and C2 is the concentration of sotolon in the substrate after adding the additional sotolon.

[0049] Table 3

[0050]

[0051] As can be seen from Table 3, the recovery rates of the determination of the yellow rice wine sample can all reach between 80% and 120%, and the RSD% is within 5%.

[0052] Subsequently, prepare a simulated yellow rice wine solution as a blank spiked substrate, add a certain amount of sotolon, and use the above treatment method for processing and detection. The results are shown in Table 4; where the preparation method of the simulated yellow rice wine solution is: mix 5 g of tartaric acid, 5 g of glucose, and 150 g of absolute ethanol, and make up the volume to 1000 mL with water.

[0053] Table 4

[0054]

[0055]

[0056] Finally, using the simulated yellow rice wine solution as the substrate, add a certain amount of sotolon to it. The obtained mixture is used as the analysis object and detected 3 times a day for 3 consecutive days. The test results are shown in Table 5; where the precision on the same day of the test results is represented by the relative standard deviation RSD of the 3 tests on the same day, and the precision across days is represented by the relative standard deviation RSD of the 3 consecutive days of tests.

[0057] Table 5

[0058] Trigonelline Intraday precision (RSD) % Interday precision (RSD) % Relative standard deviation RSD 1.48 5.12

[0059] From the above test results, it can be known that the quantitative analysis of trigonelline in the yellow rice wine of the present invention has a high accuracy rate, and the RSD of the same-day precision and the next-day precision are both within 10%, indicating that the quantitative analysis method described in this embodiment can meet the quantitative requirements of trigonelline in yellow rice wine.

[0060] Example 2

[0061] An embodiment of the method for quantitatively analyzing trigonelline in the yellow rice wine of the present invention includes the following steps:

[0062] (1) Transfer 100 mL of commercially available yellow rice wine sample Z into a 500 mL separatory funnel, add sodium chloride with a mass ratio of 4 wt% of the sample, mix well, and extract twice with 25 mL of dichloromethane. After vortex shaking for 30 s, let the mixed solution stand for stratification, collect the lower organic phase, and concentrate it quantitatively to 100 μL by nitrogen blowing to obtain concentrated solution I;

[0063] (2) Use a capillary to quantitatively spot 20 μL of concentrated solution I on a GF254 silica gel thin layer chromatography plate, and simultaneously spot the same amount of 1000 mg / mL trigonelline standard solution on the plate. Use a mixed solution prepared by mixing diethyl ether and petroleum ether 30 - 60 in a volume ratio of 0.8:1.2 as the developing agent for chromatographic separation. After the separation is completed, observe and confirm under fluorescence. Then, directly cut the silica gel at the same chromatographic distance as the standard solution to separate the active substance trigonelline. Dissolve the trigonelline in the cut silica gel with 0.25 mL of an aqueous solution of methanol with a volume content of 50%. After sucking out the re-dissolved liquid with a pipette, repeat the same operation, combine the sucked liquid with the first re-dissolved liquid, and pass it through a 0.22 μm organic membrane to obtain chromatographic treatment product II; (3) Perform UHPLC-DAD detection on chromatographic treatment product II and the trigonelline standard solution to quantitatively analyze the trigonelline in the yellow rice wine sample. The test instrument used for the UHPLC-DAD detection is an ultra-high performance liquid chromatograph Agilent 1290 Infinity, equipped with a DAD detector with a detection wavelength of 235 nm; the specific test conditions are as follows:

[0064] Time (min) A(％) B(％) 0 85 15 10 85 15

[0065] Among them, an aqueous solution of trifluoroacetic acid with a mass content of 0.12 wt% is used as mobile phase A, and methanol is used as mobile phase B, and the flow rate of the mobile phase is 0.32 mL / min;

[0066] The rest is the same as in Example 1.

[0067] Example 3

[0068] An embodiment of the method for quantitatively analyzing sotolone in Chinese rice wine according to the present invention includes the following steps:

[0069] (1) Transfer 100 mL of commercially available Chinese rice wine sample Z into a 500 mL separatory funnel, add sodium chloride with a mass ratio of 6 wt% of the sample, mix evenly, extract twice with 15 mL of dichloromethane each time. After vortex oscillation for 30 s, let the mixed solution stand for stratification, collect the lower organic phase and combine them, and quantitatively concentrate to 100 μL by nitrogen blowing to obtain concentrated solution I;

[0070] (2) Use a capillary to quantitatively spot 20 μL of concentrated solution I on a GF254 silica gel thin layer chromatography plate, and simultaneously spot the same amount of 1000 mg / mL sotolone standard solution on the plate. Use a mixed solution prepared by mixing diethyl ether and petroleum ether 30 - 60 in a volume ratio of 1.2:0.8 as the developing agent for chromatography separation. After separation, observe and confirm under fluorescence. Then, directly cut the silica gel at the same chromatography distance as the standard solution to separate the active substance sotolone. Dissolve the sotolone in the cut silica gel with 0.25 mL of an aqueous solution of methanol with a volume content of 50%. After sucking out the re-dissolved liquid with a pipette, repeat the same operation, combine the sucked liquid with the first re-dissolved liquid, and pass through a 0.22 μm organic membrane to obtain chromatographic treatment product II; (3) Perform UHPLC-DAD detection on chromatographic treatment product II and the sotolone standard solution to quantitatively analyze sotolone in the Chinese rice wine sample. The test instrument used for UHPLC-DAD is an ultra-high performance liquid chromatograph Agilent 1290 Infinity, equipped with a DAD detector with a detection wavelength of 235 nm; the specific test conditions are as follows:

[0071] Time (min) A(％) B(％) 0 85 15 10 85 15

[0072] Among them, an aqueous solution of trifluoroacetic acid with a mass content of 0.08 wt% is used as mobile phase A, and methanol is used as mobile phase B, and the flow rate of the mobile phase is 0.28 mL / min;

[0073] The rest is the same as in Example 1.

[0074] Comparative Example 1

[0075] To verify the superiority of the developing agent used in the thin-layer chromatography separation of the present invention, the yellow rice wine sample Z was subjected to thin-layer chromatography according to the methods of steps (1) and (2) described in Example 1. After the separation was completed, it was observed under fluorescence, and the test was conducted in parallel 3 times. At the same time, control groups 1 to 5 were set up. Among them, the developing agent used in control group 1 was replaced with pure acetone, the developing agent used in control group 2 was replaced with pure petroleum ether 30-60, the developing agent used in control group 3 was replaced with pure ether, the developing agent used in control group 4 was a mixed solution prepared by mixing acetone and petroleum ether 30-60 according to a volume ratio of 1:1, and the developing agent used in control group 5 was a mixed solution prepared by mixing acetone and ether according to a volume ratio of 1:1. The chromatograms of each experimental group and control group under fluorescence observation are as Figures 3 - 8 shown. It can be intuitively seen that compared with using an inappropriate or single developing agent for separation and purification, the separation effect of using a specific combination of developing agents for thin-layer chromatography in the quantitative analysis method of the present invention is better.

[0076] Comparative Example 2

[0077] To verify the superiority of using thin-layer chromatography in the quantitative analysis method of the present invention, control group 6 was set up. The difference between this control group and the method described in Example 1 was only that the treated product obtained after the concentrated solution I passed through a 0.22 μm organic membrane was directly subjected to UHPLC-DAD detection. Among them, the DAD chromatogram of the chromatographic treated product II obtained by the quantitative analysis method described in Example 1 is as Figure 9 shown, while the DAD chromatogram of the treated product obtained in control group 6 is as Figure 10 shown. By intuitive comparison, it can be seen that there are too many interfering substances in the organic phase of the concentrated sample without thin-layer chromatography treatment, making it difficult to conduct quantitative analysis of trigonelline lactone, while the interfering substances in the detected sample after treatment are significantly reduced.

[0078] Effect Example 1

[0079] To verify the universality of the quantitative analysis method of the present invention for various yellow rice wine products, various commercially available yellow rice wine products of different brands were collected according to the classification of yellow rice wine (yuanhong wine, jiafan wine, shanniang wine, and xiangxue wine), and the quantitative analysis method for trigonelline lactone in yellow rice wine of the present invention was used for detection. The test results are shown in Table 6.

[0080] Table 6

[0081]

[0082]

[0083] As can be seen from Table 6, the quantitative analysis method for trigonelline lactone in yellow rice wine of the present invention is universal for yellow rice wine products of different varieties and different trigonelline lactone concentrations, and can effectively test the specific content of trigonelline lactone in yellow rice wine.

[0084] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A quantitative analysis method for sotolon in yellow rice wine, characterized in that, It includes the following steps: (1) Take the yellow rice wine sample and extract it with an extractant, and then obtain concentrated solution I after concentration treatment; the extractant is dichloromethane, and the volume ratio of the yellow rice wine sample to dichloromethane is 1:(0.15 - 0.25); (2) Perform thin-layer chromatography treatment on concentrated solution I to obtain chromatographic treatment product II; the developing agent for the thin-layer chromatography treatment is prepared from diethyl ether and petroleum ether 30 - 60 according to the volume ratio (0.8 - 1.2):(0.8 - 1.2); the chromatographic plate used for the thin-layer chromatography treatment is a GF254 silica gel thin-layer chromatographic plate; (3) Perform UHPLC-DAD detection on chromatographic treatment product II and trigonelline standard solution to quantitatively analyze trigonelline in the yellow rice wine sample; the chromatographic column used for the UHPLC-DAD detection is an Eclipse Plus C18 series chromatographic column; during the UHPLC-DAD detection, the elution mobile phase A is an aqueous solution of trifluoroacetic acid with a mass content of 0.08 - 0.12 wt%, the mobile phase B is methanol, and the volume ratio of mobile phase A to mobile phase B is 85:

15.

2. The quantitative analysis method of sotolon in Chinese rice wine according to claim 1, characterized in that, The yellow rice wine sample includes yuanhong wine sample, jiafan wine sample, shanliang wine sample and xiangxue wine sample.

3. The quantitative analysis method of sotolon in yellow rice wine according to claim 1, characterized in that Before the extraction of the yellow rice wine sample with the extractant in step (1), sodium chloride is also added, and the addition amount of sodium chloride accounts for 4 - 6% of the mass content of the yellow rice wine sample.

4. The quantitative analysis method of sotolon in yellow rice wine according to claim 3, wherein During the UHPLC-DAD detection, the chromatographic column specification is 1.8μm 2.1mm×150mm, and the column temperature of the chromatographic column is 30 - 40°C.

5. The quantitative analysis method of sotolon in Chinese rice wine according to claim 4, wherein During the UHPLC-DAD detection, the injection volume during elution is 1.8 - 2.2 μL, and the flow rate of the mobile phase is 0.28 - 0.32 mL / min.

6. The quantitative analysis method of sotolon in yellow rice wine according to claim 1, wherein During the UHPLC-DAD detection in step (3), the DAD detection wavelength is 200 - 250 nm.

7. The quantitative analysis method of sotolon in yellow rice wine according to claim 1, characterized in that, The UHPLC-DAD detection in step (3) also includes the drawing of the trigonelline standard curve. The specific steps are as follows: Dilute and configure working solutions of trigonelline with concentrations of 1mg / mL, 5mg / mL, 10mg / mL, 40mg / mL and 100mg / mL respectively from the trigonelline standard solution, and perform UHPLC-DAD detection and draw the standard curve.

8. Application of the method for quantitative analysis of trigonelline in yellow rice wine as described in any one of claims 1 - 7 in quality control during the batch production process of yellow rice wine.

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