A pesticide composition for preventing and treating cassava red spider mites

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a kind of pesticide composition for preventing and treating cassava diamond mite. A kind of insecticide and acaricide pesticide composition, the effective component of the insecticide and acaricide pesticide composition is compounded by fluralaner and cyflumetofen ester or ethyl acetate mite. The present application screens the compound combination of fluralaner and existing pesticides, finds that the compound combination of fluralaner and cyflumetofen ester or ethyl acetate mite has a synergistic effect on diamond mite with a co-toxicity coefficient greater than 120, which can improve the control effect on diamond mite, and has important significance for preventing and controlling diamond mite on cassava. In addition, compared with a single active ingredient, the risk of drug resistance can be reduced, and the service life of the pesticide can be prolonged.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition for controlling cassava spider mite. Background Technology

[0002] The carmine spider mite [Tetranychus cinnabarinus (Boisduval)], belonging to the Tetranychidae family, is also known as the red spider mite. It is one of the most serious mite pests affecting cassava, and in severe cases, it can cause the death of the entire plant, affecting cassava yield and quality. The carmine spider mite is characterized by its small size, high reproductive capacity, and short generation cycle, with up to 15 generations per year. Currently, chemical pesticides are effective in controlling carmine spider mites on cassava. However, due to the long-term and large-scale use of chemical pesticides in production, resistance to pesticides has inevitably developed, leading to a decrease in the effectiveness of existing chemical pesticides.

[0003] Fluralaner is a novel broad-spectrum isoxazoline insecticide that acts on γ-aminobutyric acid (GABA) receptors, exhibiting good activity against pests in the orders Acari, Hemiptera, Diptera, and Lepidoptera. Fluralaner not only shows no cross-resistance with existing GABA receptor-type insecticides but is also safe for non-target organisms, demonstrating broad application prospects. Reports indicate that by testing the lethal and sublethal effects and insecticidal activity of fluralaner against the two-spotted spider mite, the results showed that fluralaner has a low LC50 against female adult two-spotted spider mites. 50 The LC50 for two-spotted spider mite eggs was 0.49 mg / L. 50 At a concentration of 0.36 mg / L, flenafil exhibits superior activity against adult female two-spotted spider mites and their eggs compared to commercially available acaricides such as pyridaben, pyridaben, and thiamethoxam. Sublethal effects studies showed that at the experimental concentration, both the lifespan and reproductive capacity of adult females were significantly reduced.

[0004] Although flenafil exhibits excellent activity against mites, long-term single application can lead to the rapid development of mite resistance. Screening flenafil combinations with other agents is crucial for controlling mites, delaying mite resistance development, and extending the lifespan of flenafil. The inventors discovered through experiments that flenafil, when combined with dicofol or etoxazole, has a synergistic effect against the target spider mite, Tetranychus carmine, improving the actual control efficacy. Currently, there are no reports on flenafil combined with dicofol or etoxazole.

[0005] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to provide a pesticide composition for controlling the cassava spider mite, in order to solve the problem that the long-term and large-scale use of chemical agents to control the cassava spider mite inevitably leads to the development of drug resistance in the cassava spider mite, resulting in a reduction in the control efficacy of existing chemical agents.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] The first objective of this invention is to provide an insecticidal and acaricidal pesticide composition, wherein the active ingredient of the insecticidal and acaricidal pesticide composition is a compound of flenafil and diflubenzuron or etoxazole.

[0009] More specifically, the mass ratio of fluranal to diflubenzuron is 1-19:9-1.

[0010] More specifically, the mass ratio of fluranal to etoxazole is 1-10:27-1.

[0011] A second objective of this invention is to provide the application of the aforementioned insecticidal and acaricidal pesticide composition in the control of cassava pests.

[0012] More specifically, the cassava pests include the carmine spider mite.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] This invention screened combinations of flenafil with existing pesticides and found that the co-toxicity coefficient of flenafil with dicofol or etoxazole against Tetranychus carinii was greater than 120, exhibiting a synergistic effect and improving the control efficacy against Tetranychus carinii, which is of great significance for controlling Tetranychus carinii on cassava. Furthermore, compared with single active ingredients, it can reduce the risk of resistance and help extend the lifespan of the pesticide. Detailed Implementation

[0015] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0016] Example Indoor bioactivity test

[0017] 1. Experimental subjects: Carmine spider mites collected from cassava planting areas were continuously reared for 8 generations in the laboratory using fresh and clean cassava leaves. Healthy and vigorous female adult mites of uniform size were selected as experimental subjects.

[0018] 2. Test reagents

[0019] 99% Fluranar (Hubei Weideli Chemical Reagent Co., Ltd.), 98% Butyric acid ester (Shandong United Pesticide Industry Co., Ltd.), 98% Ethoxyfenozide (Shenyang Kechuang Chemical Co., Ltd.)

[0020] The above-mentioned test samples were first dissolved in dimethyl sulfoxide to prepare a single-agent stock solution, and then diluted with 0.1% Tween-80 aqueous solution. Multiple formulations were set up, and five gradient mass concentrations were set for each single agent and each formulation mixture according to the proportional method for later use.

[0021] 3. Test methods (Refer to NT / T 1154.13-2008 Indoor Test Guidelines for Pesticides - Insecticides - Part 13: Leaf Disc Spraying Method)

[0022] Select fresh and clean cassava leaves and make leaf discs using a 2cm diameter punch. Place a wet sponge block in a 9cm diameter petri dish, place filter paper on it, and then place the leaf discs on the filter paper, with 2 leaf discs per dish. Inoculate the test female adult mites onto the leaf discs, with 20 mites inoculated onto each leaf disc.

[0023] Place the petri dish in a Potter spray tower (spray pressure: 1.47 × 10⁻⁶). 5 The mites were sprayed from a (Pa) tray with a spray volume of 1 mL. The solution was allowed to settle for 1 minute before being removed. Each treatment was repeated four times, with a blank control consisting of only a 0.1% Tween-80 aqueous solution. The treated female adult mites were then placed in a rearing environment with a temperature of (25±1)℃ and a photoperiod of L:D = (16:8) h. After 48 h of rearing, the mortality of the mites was observed, and the total number of mites and the number of dead mites in each treatment were recorded. The corrected mortality rate for each treatment was calculated. Mite mortality was defined as mite that did not move when touched with a soft brush on its appendages.

[0024]

[0025] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0026]

[0027] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0028] 4. Data Analysis: DPS software was used to perform regression analysis on the logarithmic values ​​of the drug concentrations for each treatment and the corrected mortality probability values ​​for each treatment, and the LC ratio of the drug for each treatment was calculated. 50 The co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method, and then the synergistic effect of the drug was evaluated based on the calculated CTC.

[0029] CTC≤80 indicates antagonistic effect, 80<CTC<120 indicates additive effect, and CTC≥120 indicates synergistic effect. The results are shown in Table 1-2.

[0030] Table 1. Toxicity test results of the combination of fluranarin and dicofol on Tetranychus carmine.

[0031]

[0032] As can be seen from Table 1, within a mass ratio of 1-19:9-1, the co-toxicity coefficient of flurranazine and diflubenzuron against Tetranychus carmine is greater than 120, indicating a synergistic effect.

[0033] Table 2. Virulence test results of flurana and etoxazole combined with Tetranychus carmine.

[0034]

[0035] As can be seen from Table 2, within a mass ratio of 1-10:27-1, the co-toxicity coefficient of fluranal and etoxazole against Tetranychus cinnabarinus is greater than 120 after being combined, indicating a synergistic effect.

[0036] In conclusion, the combination of fluranazine with dicofol or etoxazole has a co-toxicity coefficient greater than 120 against the spider mite, exhibiting a synergistic effect and improving the control efficacy against the spider mite. This is of great significance for controlling the spider mite on cassava.

[0037] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. An insecticidal and acaricidal pesticide composition, characterized in that, The active ingredient of the insecticidal and acaricidal pesticide composition is a compound of flenafil and diflubenzuron or etoxazole; wherein... The mass ratio of fluranal to fenfluridine is 1-19:9-1; The mass ratio of fluranal to etoxazole is 1-10:27-1.

2. The application of the insecticidal and acaricidal pesticide composition according to claim 1 in the control of cassava pests, characterized in that, The cassava pest is the carmine spider mite ( Tetranychus cinnabarinus ).

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