A method for extracting selenium-containing rutin from selenium-rich Moringa

Abstract

The present invention provides a method for extracting selenium-containing rutin from selenium-rich Moringa oleifera, and relates to the technical field of natural product extraction. The method comprises the following steps: (1) preparing Moringa oleifera seed powder, (2) pretreatment, and (3) extraction. The present invention proposes extracting selenium-containing rutin from selenium-rich Moringa oleifera, specifically extracting the selenium-containing rutin from selenium-rich Moringa oleifera seeds. The method has the advantages of simple process, easy operation, high extraction purity, and the like. The method of the present application can extract selenium-containing rutin substances in which selenium and rutin coexist.

Description

Technical field

[0001] The invention relates to the technical field of natural product extraction, and in particular to a method for extracting selenium-containing rutin from selenium-rich Moringa. [Background Technology]

[0002] Moringa is a perennial plant of the genus Moringa in the family Moringaceae. It is native to the southern foothills of the Himalayas in northern India. It is a fast-growing species with strong drought tolerance and prefers sandy loam soil. Its roots, stems, leaves, flowers, seeds, branches, and bark are rich in nutrients and medicinal properties. Moringa is also rich in flavonoids, polysaccharides, proteins, amino acids, and vitamins.

[0003] Rutin is a hydrogen transporter that may participate in the action of oxidoreductases in the body, affecting thyroid activity and facilitating the oxidation of adrenaline. In vivo, it enhances the effects of vitamin C and promotes its accumulation. Rutin, a flavonoid, has significant antioxidant properties. It also has anti-inflammatory, anti-allergic, diuretic, antispasmodic, antitussive, lipid-lowering, cardiotonic, and ulcer protection effects. It is primarily used clinically to prevent and treat hypertensive cerebral hemorrhage, retinal hemorrhage, hemorrhagic purpura, and acute hemorrhagic inflammation.

[0004] Selenium is an essential trace element for humans and animals. It boosts immunity and accelerates the production of immunoglobulins and antibodies. It protects the cardiovascular system and myocardium, reducing the incidence of cardiovascular disease and angina. It has multiple antioxidant, anti-aging, and cancer prevention benefits, and has special physiological functions such as reducing heavy metal toxicity. Scientists both domestically and internationally have hailed it as "the king of cancer prevention, the element of longevity, the spark of life, a natural detoxifier, and the guardian of the heart."

[0005] At present, there is no method in the prior art that can effectively extract the coexistence of selenium and rutin, two beneficial components, from Moringa seeds, and the present invention arises as the times require. [Summary of the invention]

[0006] In view of this, the object of the present invention is to provide a method for extracting selenium-containing rutin from selenium-rich Moringa, which has the advantages of simple process, easy operation, high extraction purity, etc. The method of the present application can extract selenium-containing rutin substances in which selenium and rutin coexist.

[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0008] A method for extracting selenium-containing rutin from selenium-rich Moringa oleifera comprises the following steps:

[0009] (1) preparing Moringa seed powder: taking selenium-rich Moringa seeds, removing impurities and cleaning, and then drying to obtain Moringa seed powder for later use;

[0010] (2) Pretreatment: Place the Moringa seed powder obtained in step (1) into a calcium hydroxide solution, mix thoroughly, heat and boil to dissolve, and filter to obtain the filtrate for later use;

[0011] (3) Extraction: Add ethanol solution to the filtrate obtained in step (2), and reflux extract for 35-45 minutes under heating conditions in a water bath, filter with gauze, take the filtrate, adjust the pH value of the solution with dilute hydrochloric acid, then let it stand until precipitation occurs, filter out the crystalline precipitate, wash with water, and then dry to obtain selenium-containing rutin.

[0012] In the present invention, further, the drying treatment in step (1) is: first, the Moringa seeds are placed in a condition of 40-50 ° C for warm air drying, then placed in a condition of -5 to -10 ° C for freezing treatment for 10-20 minutes, then the Moringa seeds are crushed, and finally transferred to an environment of -18 to -22 ° C for vacuum freeze drying for 1-2 hours.

[0013] In the present invention, further, after filtering in step (2), the filter residue is further heated and boiled to dissolve 2-3 times to obtain the filtrate, and the filtrate is combined.

[0014] In the present invention, further, the ethanol solution in step (3) is an 80% ethanol solution.

[0015] In the present invention, further, the dilute hydrochloric acid in step (3) is 20% dilute hydrochloric acid.

[0016] In the present invention, further, the pH of the solution in step (3) is adjusted to 3.5-4.5.

[0017] The selenium-containing rutin extracted from selenium-rich Moringa oleifera by the above method can be used as an antioxidant added to food, and can also be used to prepare vitamin medicines and health products, and has very broad application prospects.

[0018] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0019] 1. The present invention proposes a method for extracting selenium-containing rutin from selenium-rich Moringa, which specifically comprises the following steps for extracting selenium-containing rutin. The method has the advantages of simple process, easy operation, high extraction purity, etc. Selenium-containing rutin substances in which selenium and rutin coexist can be extracted by the method of the present application. The selenium-containing rutin substance can be used as an antioxidant added to food, and can also be used to prepare vitamin medicines and health products. The method has very broad application prospects and has broad market application prospects.

[0020] 2. The present application first carries out a drying process on the Moringa seeds for extracting selenium-containing rutin. The drying process is carried out by first subjecting the Moringa seeds to warm air drying. After the warm air drying, the structure inside the Moringa seeds is subject to thermal expansion. Then, the Moringa seeds are frozen at a low temperature. At this time, the internal structure of the Moringa seeds is fully and rapidly formed into a uniform crystalline structure with appropriate particle size. At this time, the Moringa seeds are crushed and then freeze-dried at a lower temperature to form a lot of uniform and dense "hole" structures inside the Moringa seeds. When immersed in a calcium hydroxide solution, the various components in the alkali solution can be promoted to be fully impregnated into the inside of the Moringa seeds. The applicant treats the Moringa seed powder with alkali solution in advance. The purpose is to remove the rich crystalline structure in the Moringa seeds. The rich and difficult-to-remove polysaccharide components make the purity of rutin higher. According to research, Moringa seeds contain a large amount of polysaccharide components. Therefore, the purity of the extraction is difficult to control. The present application dissolves rutin with alkali solution to precipitate it, which can be extracted. In addition, the calcium hydroxide at this time can form polysaccharide calcium with the polysaccharide, which is insoluble in water to form a filter residue and can be separated. The polysaccharide is separated in the process of removing the residue and retaining the liquid, and a large amount of selenium-containing rutin is precipitated in the filtrate at this time. The applicant further uses ethanol solution for extraction and finds that the extraction effect of ethanol solution with a concentration of 80% is the best. Finally, the applicant uses dilute hydrochloric acid to neutralize the filtrate, adjusts the pH, and cools it to allow the precipitate to precipitate. After centrifugation, selenium-containing rutin can be obtained. [Specific implementation method]

[0021] The following examples may help those skilled in the art to more fully understand the present invention, but are not intended to limit the present invention in any way.

[0022] Example 1

[0023] This embodiment provides a method for extracting selenium-containing rutin from selenium-rich Moringa oleifera, which specifically comprises the following steps:

[0024] (1) preparing moringa seed powder: taking selenium-rich moringa seeds, removing impurities and cleaning them, and then drying them to obtain moringa seed powder for later use; the drying treatment in step (1) is as follows: first, the moringa seeds are placed in a 40°C condition for warm air drying, then placed in a -5°C condition for freezing treatment for 10 minutes, then the moringa seeds are crushed, and finally transferred to a -18°C condition for vacuum freeze drying for 1 hour;

[0025] (2) Pretreatment: placing the Moringa seed powder obtained in step (1) into a calcium hydroxide solution, heating and boiling to dissolve after thorough mixing, filtering and taking the filtrate for standby use; after filtering in step (2), the filter residue is further heated and boiled to dissolve twice to take the filtrate, and the filtrates are combined;

[0026] (3) Extraction: Add 80% ethanol solution to the filtrate obtained in step (2), and reflux extract for 35 minutes under heating conditions in a water bath. Filter with gauze, take the filtrate, and adjust the pH value of the filtrate to 3.5 with 20% dilute hydrochloric acid. Then, let it stand until precipitation occurs, filter out the crystalline precipitate, wash with water, and dry it to obtain selenium-containing rutin.

[0027] Example 2

[0028] This embodiment provides a method for extracting selenium-containing rutin from selenium-rich Moringa oleifera, which specifically comprises the following steps:

[0029] (1) preparing moringa seed powder: taking selenium-rich moringa seeds, removing impurities and cleaning them, and then drying them to obtain moringa seed powder for later use; the drying process in step (1) is as follows: first, the moringa seeds are placed in a 45°C condition for warm air drying, then placed in a -7°C condition for freezing treatment for 15 minutes, then the moringa seeds are crushed, and finally transferred to a -20°C condition for vacuum freeze drying for 1.5 hours;

[0030] (2) Pretreatment: placing the Moringa seed powder obtained in step (1) into a calcium hydroxide solution, heating and boiling to dissolve after thorough mixing, filtering and taking the filtrate for standby use; after filtering in step (2), the filter residue is further heated and boiled to dissolve twice to take the filtrate, and the filtrates are combined;

[0031] (3) Extraction: Add 80% ethanol solution to the filtrate obtained in step (2), and reflux extract for 40 minutes under heating conditions in a water bath. Filter with gauze, take the filtrate, and adjust the pH value of the filtrate to 4.0 with 20% dilute hydrochloric acid. Then, let it stand until precipitation occurs, filter out the crystalline precipitate, wash with water, and then dry it to obtain selenium-containing rutin.

[0032] Example 3

[0033] This embodiment provides a method for extracting selenium-containing rutin from selenium-rich Moringa oleifera, which specifically comprises the following steps:

[0034] (1) preparing moringa seed powder: taking selenium-rich moringa seeds, removing impurities and cleaning them, and then drying them to obtain moringa seed powder for later use; the drying process in step (1) is as follows: first, the moringa seeds are placed in a 50°C condition for warm air drying, then placed in a -10°C condition for freezing treatment for 20 minutes, then the moringa seeds are crushed, and finally transferred to a -22°C condition for vacuum freeze drying for 2 hours;

[0035] (2) Pretreatment: placing the Moringa seed powder obtained in step (1) into a calcium hydroxide solution, fully mixing, heating and boiling to dissolve, filtering and taking the filtrate for standby use; after filtering in step (2), the filter residue is further heated and boiled to dissolve three times to obtain the filtrate, and the filtrates are combined;

[0036] (3) Extraction: Add 80% ethanol solution to the filtrate obtained in step (2), and reflux extract for 45 minutes under heating conditions in a water bath. Filter with gauze, take the filtrate, and adjust the pH value of the filtrate to 4.5 with 20% dilute hydrochloric acid. Then, let it stand until precipitation occurs, filter out the crystalline precipitate, wash with water, and dry it to obtain selenium-containing rutin.

[0037] Test example

[0038] In order to illustrate the practical value of this application, the applicant conducted the following comparative tests in several groups:

[0039] Group 1: selenium-containing rutin prepared by the method described in Example 2;

[0040] Group 2: The drying step was removed, and the other steps were strictly followed as in Example 2;

[0041] Group 3: The step of soaking in calcium hydroxide solution was removed, and the other methods were strictly followed as in Example 2;

[0042] Group 4: The drying process was changed to: first, the Moringa seeds were frozen at -7°C for 15 min, then the Moringa seeds were crushed, and finally, vacuum freeze-dried at -20°C for 1.5 h. Other steps were strictly followed according to Example 2.

[0043] Group 5: The drying process was changed as follows: first, the Moringa seeds were crushed, then dried with warm air at 45°C, then frozen at -7°C for 15 min, and finally vacuum freeze-dried at -20°C for 1.5 h. Other steps were strictly followed those in Example 2.

[0044] Group 6: The ethanol solution was 95% ethanol solution, and other methods were strictly carried out according to Example 2.

[0045] Comparing the extraction effects of selenium-containing rutin in the above groups, the recorded data are shown in Table 1:

[0046] Table 1 Comparison of extraction effects of rutin containing selenium

[0047]

[0048] As can be seen from Table 1, the extraction rate of selenium-containing rutin from selenium-rich Moringa by the method of the present application is the highest, and the purity of the obtained selenium-containing rutin is the highest.

[0049] Although the present invention has been described in detail above using general descriptions and specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made based on the present invention. Therefore, such modifications and improvements, which do not depart from the spirit of the present invention, are intended to be within the scope of protection claimed herein.

Claims

1. A method for extracting selenium-containing rutin from selenium-rich Moringa, characterized in that, The following steps are involved: (1) Preparation of Moringa Seed Powder: Selenium-rich Moringa seeds are removed from the seeds and cleaned, and then dried to obtain Moringa Seed Powder for later use; the drying process is as follows: first, the Moringa seeds are placed in a warm air drying condition at 40-50°C, then frozen at -5 to -10°C for 10-20 minutes, then the Moringa seeds are crushed, and finally transferred to a vacuum freeze drying condition at -18 to -22°C for 1-2 hours; (2) Pretreatment: Place the Moringa seed powder obtained in step (1) into a calcium hydroxide solution, mix thoroughly, heat and boil to dissolve, and filter to obtain the filtrate for later use; (3) Extraction: Add 80% ethanol solution to the filtrate obtained in step (2), and reflux extract for 35-45 minutes under heating conditions in a water bath. Filter with gauze, take the filtrate, and adjust the pH value of the solution with dilute hydrochloric acid. Then, let it stand until precipitation occurs. Filter the crystalline precipitate, wash with water, and dry it to obtain selenium-containing rutin. After filtering in step (2), the process further includes repeatedly heating and boiling the filter residue to dissolve it 2-3 times to obtain the filtrate, and combining the filtrates; the dilute hydrochloric acid in step (3) is 20% dilute hydrochloric acid; and the pH of the solution in step (3) is adjusted to 3.5-4.

5.

2. Selenium-containing rutin extracted by the method according to claim 1.

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