A repairing and anti-aging facial cream containing jasmine flower extract

Abstract

The invention discloses a repairing and anti-aging facial cream containing jasmine extract, wherein the main components include 10-25% of effective components, 60-70% of deionized water, 4-6% of glycerol, 0.05-0.2% of xanthan gum, 0.05-0.1% of EDTA-2Na, 0.1-0.3% of sodium hyaluronate, 2-3% of Tween-60, 0.5-1.5% of Span-60, 2-4% of white oil, 2-4% of squalane, 1-3% of DM100, 2-4% of IPM, 3-5% of GCTT, 1-3% of vaseline, 0.1-0.3% of tocopherol, 0.5-0.7% of AHEG and 0.5-1% of EMT-10. The effective components are extracted from jasmine, clematis, pawpaw tree, kelp vine and camellia oleifera. The repairing and anti-aging facial cream containing jasmine flower extract prepared by the present invention can improve the oil and water content and sensitivity problems of the skin, is refreshing and non-greasy, is quickly absorbed, has good whitening and spot-removing and anti-aging effects, and the fragrance of the jasmine flower extract itself can make people feel happy and invigorate.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a repairing and anti-aging facial cream containing jasmine extract and a preparation method thereof. Background Art

[0002] With the economic growth, people have become more aware of beauty and their requirements for skin care products have gradually increased after satisfying their basic needs. People today not only need effective skin care products, but also low-irritation and safer skin care products. Therefore, extracting effective ingredients from plants and preparing high-efficiency and low-irritation products is the future trend.

[0003] Water is the most basic compound that constitutes life. The skin contains a lot of water. The standard for healthy skin is 10% to 20% water. If the water-oil balance is broken, the skin will lack water or oil, or the skin will be oily and cause pores to be clogged, resulting in acne, blackheads, etc., and skin barrier problems will follow. The thickness of the human epidermis is between 0.05 and 1.5 mm. Although the thickness is not thick, it has an irreplaceable position as the first line of defense of the human body and forms an important line of defense for the human body. The human skin barrier can not only prevent the loss of substances such as water in the body, but also prevent the invasion of external substances such as bacteria. When the skin barrier is damaged, not only the skin's moisturizing ability decreases, but also skin diseases and sub-health conditions will occur, manifested as dry, tight, red skin, poor irritation tolerance, and easy allergic reactions. Reduced skin moisturizing ability will lead to damaged skin barrier, and damaged skin barrier will further lead to reduced skin moisturizing ability, forming a vicious circle. Therefore, for normal skin barrier function, moisturizing is both to maintain the physiological function of the skin and delay skin aging. Moisturizing is both the foundation and the top priority.

[0004] Skin aging refers to the gradual functional and organic degenerative changes of the skin due to the passage of events, which are manifested as wrinkles, dryness, flaking, roughness, sagging and spots, slowed epidermal renewal, weakened barrier function, decreased activity of stratum corneum cells, reduced synthesis of collagen and elastin, increased decomposition, and skin becomes loose and loses elasticity. Collagen is the most abundant protein in the human body and an important component of the extracellular matrix (ECM). It plays an important role in the adhesion of the epidermal basal layer and the dermis. It is an important substance for maintaining the morphology and structure of the skin and can give the skin toughness and elasticity. The reduction of collagen is one of the important characteristics of aging skin. Free radicals are the remains of human metabolism, also known as reactive oxygen species. They are necessary for maintaining normal life activities and have the functions of phagocytosis, detoxification and synthesis of various substances required by the human body. However, when there are too many free radicals in the human body and the balance is lost, it will cause various harms to the human body. Free radicals may cause stress reactions, cancer, and damage protein cell membranes. Therefore, the balance of free radicals in the human body is very important. The free radical aging theory has attracted much attention in the theory of human aging. Modern research shows that free radicals may not be directly related to aging, but aging may occur when its dynamic balance is broken.

[0005] Most anti-aging products on the market contain retinol, peptides, and bosine. Although their mechanisms of action are different, they ultimately directly or indirectly promote the synthesis of collagen to resist aging. However, they still have the disadvantages of being unfriendly to sensitive skin, slow effect, sticky texture, or slow absorption, and anti-aging products are all used to resist aging from a single aspect, which has limitations. Jasmine is widely distributed in southern my country and plays an important role in both edible and pharmacological aspects. Jasmine extract contains a variety of compounds that can remove free radicals and inhibit the activity of MMP to reduce the degradation of collagen. Compared with general anti-aging and antioxidant ingredients, jasmine extract can repair the skin barrier, improve skin sensitivity, and is more suitable for sensitive skin. However, jasmine extract also has poor stability, is easily volatilized, and is inactivated by the influence of external temperature. For this reason, reduced pressure steam distillation is used in the present invention to reduce the distillation temperature and reduce inactivation. The extracted essential oil is decolorized by a composite decolorizer, and then filtered to obtain clarified essential oil, and embedded in the subsequent process so that the jasmine extract can play a better role. Summary of the invention

[0006] In view of this, the object of the present invention is to provide a repairing and anti-aging facial cream containing jasmine extract.

[0007] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0008] A repairing and anti-aging facial cream containing jasmine extract, the raw material components of which are measured by weight:

[0009] 10~25 parts of active ingredients;

[0010] 60~70 parts of deionized water;

[0011] 4-6 parts of glycerin;

[0012] Xanthan gum 0.05~0.2 parts;

[0013] EDTA-2Na 0.05~0.1 parts;

[0014] Sodium hyaluronate 0.1-0.3 parts;

[0015] 1~2 parts of Camellia oleifera polypeptide;

[0016] Tween-602~3 copies;

[0017] Span - 600.5~1.5 copies;

[0018] 2~4 parts of white oil;

[0019] 2-4 parts of squalane;

[0020] DM100 1~3 parts;

[0021] IPM2~4 copies;

[0022] GCTT 3~5 copies;

[0023] 1~3 parts of Vaseline;

[0024] Tocopherol 0.1~0.3 parts;

[0025] AHEG 0.5~0.7 parts;

[0026] EMT-100.5~1 copy;

[0027] Wherein, the effective ingredient comprises jasmine extract.

[0028] As a possible implementation mode, further, the effective ingredients include the following raw material extracts in parts by weight:

[0029] 40-50 parts of jasmine extract;

[0030] 30-40 parts of Pawpaw fruit extract;

[0031] 30-40 parts of Clematis chinensis extract;

[0032] 30-40 parts of Phellodendron chinense extract.

[0033] As a preferred embodiment, preferably, the preparation method of the jasmine extract in the effective ingredients includes:

[0034] 5-15 parts of activated clay, 1-5 parts of activated carbon, 3-10 parts of diatomaceous earth and 1-5 parts of tourmaline powder are mixed and stirred evenly to obtain a composite decolorizing agent, 15-30% of the volume of the composite decolorizing agent is added to jasmine essential oil, and the mixture is stirred in a closed manner at 45-55° C. for 10-20 minutes, and the obtained solution is filtered to obtain a clarified essential oil, namely, a jasmine extract.

[0035] As a preferred embodiment, the preparation method of the jasmine essential oil preferably comprises:

[0036] Pick jasmine flowers between 2 and 6 p.m., and keep them indoors for 2 to 4 hours between 6 and 12 a.m. at a temperature of 25 to 35 degrees Celsius, then wash and remove impurities from the jasmine flowers;

[0037] Fresh jasmine flowers were freeze-dried, crushed through a 150-mesh sieve, and placed in an ultrasonic cell disruptor. Deionized water was then added according to a solid-liquid ratio of 1:3, and ultrasonic treatment was performed for 1 to 1.5 hours at a temperature not exceeding 30°C.

[0038] The treated solution was added with cellulase and pectinase loaded with O-Fe3O4, and enzymolysis was carried out at 50°C and pH = 4.8 for 12 h;

[0039] The solution was then loaded into a distillation tank, an appropriate amount of deionized water was added, and the distillation tank cover with a distillation tube was covered for reduced pressure steam distillation. The steam volume was set to 200 L / h, the vacuum degree was 13.33 kPa, and the extraction time was 2.5 h. After oil and water separation, anhydrous Na2SO4 was added to the separated oil layer to absorb water, and the mixture was left overnight. Finally, the solid Na2SO4 was filtered out to obtain jasmine essential oil.

[0040] As a preferred embodiment, the method for preparing O-Fe3O4 loaded with cellulase and pectinase comprises:

[0041] 200mL nFe 3+ :nFe 2+ =1.5:1 mixed solution was placed in a three-necked flask, and 18 mL NH3 (25%, v / v) was quickly added at 70°C under N2 protection and high-speed stirring. After 1 min, 4.66 g oleic acid was added dropwise;

[0042] Maintain the reaction temperature and continue to stir rapidly for 1 h to obtain a black colloidal substance. Use a Nd-Fe-B magnet to separate the product from the reaction system, wash the product to neutrality, then add 160 mL, 10 mg / mL KMnO4 solution, ultrasonically shake for 8 h, filter and wash to neutrality, and vacuum dry for 40 h to obtain carboxylated Fe3O4 magnetic nanoparticles, labeled: O-Fe3O4;

[0043] The O-Fe3O4 carrier was dispersed in 10 mL of cellulase and pectinase solution with a pH of 4.8, placed in a constant temperature water bath oscillator, and oscillated at 25°C and 150 r / min. Finally, the immobilized cellulase and pectinase were separated using a Nd-Fe-B magnet to obtain O-Fe3O4-loaded cellulase and pectinase.

[0044] As a preferred embodiment, preferably, the method for preparing the camellia oleifera polypeptide comprises:

[0045] Weigh 10g of defatted tea oil meal and crush it until it passes through an 80-mesh sieve;

[0046] Add 200 mL of distilled water for soaking, and add 1 mol / L sodium hydroxide to adjust the pH to 8. Place in a 60°C water bath and heat for 80 min. Transfer to a centrifuge and centrifuge at 4000 r / min for 20 min.

[0047] The supernatant was taken, 1 mol / L HCl was added to adjust the pH to 3 to the isoelectric point, and then centrifuged at 4000r / min for 20min. After centrifugation, obvious stratification can be observed. The upper liquid was discarded, and the lower material was the extracted camellia meal protein; finally, the camellia protein was obtained by freeze drying;

[0048] Distilled water with a solid-liquid ratio of 1:25 was added to the extracted camellia oleifera protein, and then 1 mol / L HCl was added to adjust the pH to 10 and heated to 90°C for extraction for 20 minutes. Then 1 mol / L HCl was used to adjust the pH to 6.5, and papain was added. The enzymatic hydrolysis conditions were: temperature 54°C, enzyme amount 4U / g, and enzymatic hydrolysis time 4 hours. After enzymatic hydrolysis, the enzyme was inactivated by boiling water bath method for 30 minutes. Then the protein was transferred to a centrifuge and centrifuged at 4000r / min for 15 minutes. Stratification was observed, and the upper clear liquid was freeze-dried to obtain camellia oleifera polypeptide.

[0049] As a preferred embodiment, preferably, the preparation method of the Pawpaw tree fruit extract in the effective ingredients includes:

[0050] Weigh 100 g of Pawpaw fruit for drying treatment, wash, dry, peel, remove the core of the Pawpaw fruit, and pulp it with a fruit crusher, then place it in a distillation tank, add deionized water at a solid-liquid ratio of 1:5, soak it at room temperature for 6 hours, perform reduced pressure steam distillation, heat the liquid for extraction for 5 hours, obtain an extract, then evaporate the water of the extract and concentrate it into a paste to obtain a Pawpaw fruit extract rich in chlorogenic acid.

[0051] As a preferred embodiment, preferably, the preparation method of the Clematis chinensis extract in the effective ingredients includes:

[0052] Weigh 100 g of Clematis chinensis and dry it. Add 300 mL of distilled water to the Clematis chinensis powder passed through a 20-mesh sieve, heat under reflux to obtain a brown-black liquid that is slightly turbid. Take the extract and evaporate the water to obtain a brown viscous solid to obtain the Clematis chinensis extract.

[0053] As a preferred embodiment, preferably, the preparation method of the extract of the calendula officinalis in the effective ingredients includes:

[0054] The dried kelp was crushed into 80-100 meshes, 100 g of kelp powder was weighed, 300 mL of distilled water was added, and the mixture was heated under reflux to obtain an extract, and the extract was evaporated to remove water to obtain a viscous solid, thereby obtaining a kelp extract.

[0055] The present invention also provides a method for preparing a repairing and anti-aging facial cream containing jasmine extract, which is characterized by comprising the following steps:

[0056] S1: Add deionized water, glycerin, xanthan gum, EDTA-2Na, sodium hyaluronate, and camellia oleifera polypeptide into an emulsification tank, and heat to 85-95°C while stirring to obtain phase B. Add Tween-60, Span-60, white oil, squalane, DM100, IPM, GCTT, vaseline, and tocopherol into an emulsification tank, and heat to 85-95°C while stirring to obtain phase C. After stirring evenly, mix phases B and C, continue to stir evenly, then add AHEG, and continue to stir for 0.5h-1h to obtain mixture D.

[0057] S2: Cool the product D obtained in step S1 to 50-55° C., add mixture A and mixture E, and stir evenly to obtain a mixed solution F.

[0058] S3: Cool the mixture F obtained in step S3 to 35-45° C., add EMT-10, stir evenly and cool to obtain cream G;

[0059] The preparation method of the mixture E comprises:

[0060] The jasmine extract, emulsifier and deionized water in a mass ratio of 1:0.04-0.06:20-50 are stirred and emulsified to obtain an emulsion, and then a 10-20% calcium chloride aqueous solution is added dropwise. After the addition is completed, stirring is continued to obtain a mixture, and a 10-20% sodium carbonate aqueous solution is added to the obtained mixed solution at a dropping rate of 2-5 mL / min. After the addition is completed, stirring is continued to react, and the solid is filtered under reduced pressure and dried to obtain jasmine essential oil microcapsules, namely, mixture E;

[0061] The mixture A is a water solution prepared by mixing jasmine flower extract, P. sambac fruit extract, clematis root extract, oleifera oleifera extract, camellia oleifera polypeptide and water.

[0062] Jasmine extract contains a variety of organic compounds such as esters, alcohols, terpenes, ketones, aldehydes, etc. It has effective pharmacological effects in the treatment fields of repair, moisturizing, anti-oxidation, and anti-aging. Modern pharmacology shows that ketones and polyphenols are both biologically strong antioxidants, and have good whitening and anti-aging effects, and effectively remove various free radicals. It improves the oil and water content of the skin and sensitive problems, is refreshing and non-greasy, and absorbs quickly. The aroma of jasmine extract itself will make people feel happy and invigorate.

[0063] The extract of P. chinensis contains chlorogenic acid, caffeoylchlorogenic acid, caffeoylquinic acid and several caffeoyl derivatives; it has a high content of fatty acids. In addition to common saturated fatty acids and unsaturated fatty acids, the unsaturated fatty acids with special structures include cis-9-hexadecenoic acid, cis-16-hexadecenoic acid, cis-13-oleic acid and cis, cis-9,13-dienoic acid, etc. It has a strong ability to eliminate free radicals and is often used as a cosmetic antioxidant.

[0064] The extract of Leptochloa oleifera contains triptolide β-amyrin, β-amyrin palmitate, syringic acid, p-hydroxybenzaldehyde, vanillic acid, β-sitosterol, carrot glycosides, etc. The extract of Leptochloa oleifera contains anti-Gram-positive cocci substances, which play an important role in wound healing after skin damage, have anti-inflammatory and antibacterial effects, and promote wound scarring. It has an inhibitory effect on the activity of caspase, prolongs cell life, enhances cell activity, and has an anti-aging effect.

[0065] Clematis extract has good moisturizing ability, can increase skin elasticity, remove some aging cuticles in the skin, promote skin metabolism, and effectively improve various fine lines and wrinkles on the face. It has an inhibitory effect on Staphylococcus aureus, Gram-negative bacteria and other bacteria, soothes and resists allergies, improves and repairs skin barriers, etc.

[0066] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0067] 1) The functional ingredients added in the present invention have good moisturizing and repairing effects, scavenging free radicals, and reducing the decomposition of collagen. The compounding of the functional ingredients is in accordance with a certain ratio and formula, and a suitable base oil is selected to blend together, and the remaining natural plant essential oil ingredients are combined to enhance the efficacy while increasing the action pathway. The present invention has a reasonable formula and has a good effect on anti-inflammatory and whitening.

[0068] 2) The essential oil prepared by the present invention is lipophilic and easily soluble in oils and fats, because the molecular chain of essential oils is usually short and the molecules are small, which makes them very easy to penetrate the skin and can be well absorbed and exert their effects. Pure and natural essential oils are active and have healing effects. They can exert their effects without irritating the skin, are friendly to sensitive skin, and the cream is refreshing and non-greasy, and is also suitable for oily skin.

[0069] 3) The present invention uses an ultrasonic pulverizer to pre-treat the effective ingredients, which will not cause raw material loss, does not add other chemical reagents, and more effectively retains the active ingredients therein, thereby improving the utilization rate of the medicinal materials. By using reduced pressure steam distillation to reduce the distillation temperature, extraction can be performed at a relatively low temperature, reducing the inactivation of the active ingredients, and the aroma is closer to the original aroma of jasmine. This method has the advantages of high oil yield, good aroma retention, simple process, low cost, short extraction time, low energy consumption, etc., and is suitable for industrial production.

[0070] 4) In the present invention, a composite decolorizing agent is used to remove the discolored part of the essential oil, and the microcapsule technology is used to embed the effective component extract. Jasmine essential oil has poor stability, is easily volatilized and affected by external temperature. After microcapsule embedding, the solubility of jasmine essential oil in water can be increased, and a slow-release effect can be produced, which improves its stability to light, heat and oxidation.

[0071] 5) In the present invention, cellulase and pectinase are loaded on O-Fe3O4, and the immobilized cellulase and pectinase are separated by Nd-Fe-B magnet, which is convenient to use and separate.

[0072] 6) The ingredients of the present invention are extracted from jasmine, pawpaw, kelp and clematis, which contain repairing, whitening and anti-aging factors. The production process is less difficult and the extraction steps of the effective ingredients are simple. Compared with the existing repairing and anti-aging products, it is highly efficient and non-irritating. At the same time, it does not contain raw materials that are irritating to the skin, but can also achieve good repairing and anti-aging effects. DETAILED DESCRIPTION

[0073] The present invention will be further described in detail below in conjunction with the embodiments. It is particularly noted that the following embodiments are only used to illustrate the present invention, but are not intended to limit the scope of the present invention. Similarly, the following embodiments are only partial embodiments of the present invention rather than all embodiments, and all other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of protection of the present invention.

[0074] Example 1

[0075] The repairing and anti-aging facial cream containing jasmine extract in this embodiment comprises the following raw materials in parts by weight:

[0076] 10 parts of active ingredients;

[0077] 70 parts of deionized water;

[0078] Glycerin 5 parts;

[0079] Xanthan gum 0.05~0.2 parts;

[0080] EDTA-2Na 0.05~0.1 parts;

[0081] Sodium hyaluronate 0.1-0.3 parts;

[0082] 1~2 parts of Camellia oleifera polypeptide;

[0083] Tween - 602.1 copies;

[0084] Span - 600.9 copies;

[0085] 3 parts white oil;

[0086] 3 parts of squalane;

[0087] DM1002 copies;

[0088] IPM 3 copies;

[0089] GCTT 4 copies;

[0090] 2 parts Vaseline;

[0091] Tocopherol 0.2 parts;

[0092] AHEG 0.6 parts;

[0093] EMT - 100.88 copies;

[0094] Among them, the effective ingredients include the following raw material extracts in parts by weight:

[0095] 40 parts of jasmine extract;

[0096] 30 parts of Pawpaw fruit extract;

[0097] 30 parts of Clematis extract;

[0098] 30 parts of Phellodendron chinense extract.

[0099] The preparation method of the camellia oleifera polypeptide in this embodiment includes:

[0100] Weigh 10g of defatted camellia oleifera meal and crush it until it passes through a 80-mesh sieve; add 200mL of distilled water to soak, adjust the pH to 8 with 1 mol / L sodium hydroxide, place in a 60℃ water bath and continue heating for 80 min. After the end, transfer to a centrifuge at a speed of 4000 r / min and centrifuge for 20 min; take the supernatant, add 1 mol / L HCl to adjust the pH to 3 to the isoelectric point, and then centrifuge at the same speed of 4000r / min for 20min. After centrifugation, obvious stratification can be observed. Discard the upper liquid and the lower material is the extracted camellia oleifera meal protein; finally, freeze-dry to obtain camellia oleifera protein and weigh it.

[0101] Add distilled water with a solid-liquid ratio of 1:25 to the extracted camellia oleifera protein, add 1 mol / L HCl after adding distilled water to adjust the pH to 10 and heat, heat to 90°C and extract for 20 minutes, adjust the pH to 6.5 with 1 mol / L HCl, add papain, and the enzymatic hydrolysis conditions are: temperature 54°C, enzyme amount 4U / g, and enzymatic hydrolysis time 4 hours; after enzymatic hydrolysis, inactivate the enzyme by boiling water bath for 30 minutes; transfer to a centrifuge and centrifuge at a speed of 4000r / min for 15 minutes, observe stratification, and take the upper clear liquid for freeze-drying to obtain the camellia oleifera polypeptide.

[0102] In addition, the preparation method of the functional component of this embodiment includes:

[0103] (1) Preparation of jasmine extract: jasmine flowers are picked between 2 and 6 p.m., and are kept indoors in a bamboo basket for 2 to 4 hours at 6 to 12 a.m. at a temperature of 25 to 35°C. When the jasmine flowers are fragrant, the fresh jasmine flowers are washed and cleaned; fresh jasmine flowers are freeze-dried, crushed through a 150-mesh sieve, placed in an ultrasonic cell disruptor, and deionized water in a ratio of 1:3 is added, and ultrasonic treatment is performed for 1 hour to 1.5 hours at a temperature not exceeding 30°C. The treated solution was added with cellulase and pectinase loaded with O-Fe3O4, and enzymolysis was performed at a temperature of 50°C and pH = 4.8 for 12 hours. The solution was then placed in a distillation tank, an appropriate amount of deionized water was added, and a distillation tank cover provided with a distillation tube was covered to perform reduced pressure steam distillation, with the steam volume set at 200L / h, the vacuum degree set at 13.33kPa, and the extraction time set at 2.5h. After oil-water separation, anhydrous Na2SO4 was added to the separated oil layer, water was absorbed, and the mixture was left overnight, and solid Na2SO4 was filtered out to obtain jasmine essential oil;

[0104] Take 5-15 parts of activated clay, 1-5 parts of activated carbon, 3-10 parts of diatomaceous earth, and 1-5 parts of tourmaline powder, mix and stir evenly to obtain a composite decolorizing agent, add 15-30% of the composite decolorizing agent by volume to the obtained jasmine essential oil, and stir in a closed manner at 45-55° C. for 10-20 minutes. Filter the obtained solution to obtain a clarified essential oil, i.e., a jasmine extract.

[0105] The preparation method of the above-mentioned O-Fe3O4 loaded cellulase and pectinase comprises:

[0106] 200mL nFe 3+ :nFe 2+ =1.5:1 mixed solution was placed in a three-necked flask, and 18 mL NH3 (25%, v / v) was quickly added under N2 protection and high-speed stirring at 70°C. After 1 min, 4.66 g oleic acid was added dropwise. The reaction temperature was maintained and the reaction was continued to be stirred rapidly for 1 h to obtain a black colloidal substance. The product was separated from the reaction system with a Nd-Fe-B magnet, and the product was washed to neutrality. Then 160 mL, 10 mg / mL KMnO4 solution was added, ultrasonically shaken for 8 h, filtered and washed to neutrality, and vacuum dried for 40 h to obtain carboxylated Fe3O4 magnetic nanoparticles, marked as: O-Fe3O4; the O-Fe3O4 carrier was dispersed in 10 mL, pH=4.8 cellulase and pectinase solution, placed in a constant temperature water bath oscillator, 25°C, 150 r / min oscillation reaction, and finally using Nd-Fe-B magnet to separate the immobilized cellulase and pectinase to obtain O-Fe3O4 loaded cellulase and pectinase.

[0107] (2) Preparation of Pawpaw fruit extract: Weigh 100 g of Pawpaw fruit and dry it. Wash the Pawpaw fruit, air dry, peel and pit it, and beat it with a fruit crusher. Then put it in a distillation tank, add 1:5 deionized water, soak it at room temperature for 6 hours, perform reduced pressure steam distillation, heat the liquid, extract it for 5 hours, and obtain an extract. The water content of the extract is evaporated and concentrated into a paste to obtain a Pawpaw fruit extract rich in chlorogenic acid.

[0108] (3) Preparation of Clematis chinensis extract: Weigh 100 g of Clematis chinensis and dry it. Add 300 mL of distilled water to the Clematis chinensis powder that has passed through a 20-mesh sieve. Heat and reflux to obtain a brown-black liquid that is slightly turbid. Take the extract and evaporate the water to obtain a brown viscous solid, which is the Clematis chinensis extract.

[0109] (4) Preparation of the extract of the Ligusticum chuanxiong: The dried Ligusticum chuanxiong was crushed into 80-100 mesh size, 100 g of the Ligusticum chuanxiong powder was accurately weighed, 300 mL of distilled water was added, and the mixture was heated under reflux to obtain an extract. The extract was evaporated to remove water to obtain a viscous solid, i.e., the Ligusticum chuanxiong extract.

[0110] This embodiment also provides a method for preparing the above-mentioned repairing and anti-aging facial cream containing jasmine extract, which comprises the following steps:

[0111] S1: Add deionized water, glycerin, xanthan gum, EDTA-2Na, sodium hyaluronate and camellia oleifera polypeptide into an emulsification tank, heat to 85-95°C while stirring to obtain phase B; add Tween-60, Span-60, white oil, squalane, DM100, IPM, GCTT, vaseline and tocopherol into an emulsification tank, heat to 85-95°C while stirring to obtain phase C; mix phases B and C after stirring evenly, continue stirring evenly, then add AHEG, and continue stirring for 0.5h-1h to obtain compound D.

[0112] S2: Stir and emulsify jasmine essential oil, emulsifier and deionized water in a mass ratio of 1:0.04-0.06:20-50 to obtain an emulsion, and then drip a 10-20% calcium chloride aqueous solution. After the dripping is completed, continue to stir to obtain a mixture. Add a 10-20% sodium carbonate aqueous solution to the obtained mixed solution at a drip rate of 2-5 mL / min, and continue to stir the reaction after the dripping is completed. After vacuum filtration and drying of the obtained solid, jasmine essential oil microcapsules, namely mixture E, are obtained.

[0113] S3: Cool the product D obtained in step S1 to 50-55° C., add the mixture A and the mixture E, stir evenly, and obtain a mixed solution F.

[0114] S4: Cool the mixture F obtained in step S3 to 35-45° C., add EMT-10, stir evenly and cool to obtain cream G.

[0115] Wherein, mixture A is a water solution prepared by mixing jasmine flower extract, Pawpaw tree fruit extract, Clematis chinensis extract, Perilla frutescens extract, Camellia oleifera polypeptide and water.

[0116] Example 2

[0117] This embodiment is substantially the same as the above-mentioned embodiment 1, except that the cream formula and the functional ingredient formula of this embodiment are different, as follows:

[0118] The repairing and anti-aging facial cream containing jasmine extract in this embodiment comprises the following raw materials in parts by weight:

[0119] 15 active ingredients;

[0120] 65 parts of deionized water;

[0121] Glycerin 5 parts;

[0122] Xanthan gum 0.05~0.2 parts;

[0123] EDTA-2Na 0.05~0.1 parts;

[0124] Sodium hyaluronate 0.1-0.3 parts;

[0125] 1~2 parts of Camellia oleifera polypeptide;

[0126] Tween - 602.1 copies;

[0127] Span - 600.9 copies;

[0128] 3 parts white oil;

[0129] 3 parts of squalane;

[0130] DM1002 copies;

[0131] IPM 3 copies;

[0132] GCTT 4 copies;

[0133] 2 parts Vaseline;

[0134] Tocopherol 0.2 parts;

[0135] AHEG 0.6 parts;

[0136] EMT - 100.88 copies;

[0137] Among them, the effective ingredients include the following raw material extracts in parts by weight:

[0138] 45 parts of jasmine extract;

[0139] 35 parts of Pawpaw fruit extract;

[0140] 35 parts of Clematis extract;

[0141] 35 parts of Phellodendron chinense extract.

[0142] The preparation method of the camellia oleifera polypeptide, the preparation method of the functional ingredient, and the preparation method of the repair and anti-aging facial cream containing jasmine extract in this embodiment are the same as those in Example 1, and will not be described in detail.

[0143] Example 3

[0144] This embodiment is substantially the same as the above-mentioned embodiment 1, except that the cream formula and the functional ingredient formula of this embodiment are different, as follows:

[0145] The repairing and anti-aging facial cream containing jasmine extract in this embodiment comprises the following raw materials in parts by weight:

[0146] 20 servings of active ingredients;

[0147] 60 parts of deionized water;

[0148] Glycerin 5 parts;

[0149] Xanthan gum 0.05~0.2 parts;

[0150] EDTA-2Na 0.05~0.1 parts;

[0151] Sodium hyaluronate 0.1-0.3 parts;

[0152] 1~2 parts of Camellia oleifera polypeptide;

[0153] Tween - 602.1 copies;

[0154] Span - 600.9 copies;

[0155] 3 parts white oil;

[0156] 3 parts of squalane;

[0157] DM1002 copies;

[0158] IPM 3 copies;

[0159] GCTT 4 copies;

[0160] 2 parts Vaseline;

[0161] Tocopherol 0.2 parts;

[0162] AHEG 0.6 parts;

[0163] EMT - 100.88 copies;

[0164] Among them, the effective ingredients include the following raw material extracts in parts by weight:

[0165] Jasmine extract 50 parts;

[0166] 40 parts of Pawpaw fruit extract;

[0167] 40 parts of Clematis extract;

[0168] 40 parts of Phellodendron chinense extract.

[0169] The preparation method of the camellia oleifera polypeptide, the preparation method of the functional ingredient, and the preparation method of the repair and anti-aging facial cream containing jasmine extract in this embodiment are the same as those in Example 1, and will not be described in detail.

[0170] Comparative Example 1

[0171] The difference between the repairing and anti-aging facial cream containing jasmine extract in this comparative example and Example 1 is that the raw material for extracting the effective ingredients does not contain jasmine extract, and the other formula components, addition amounts and preparation methods are the same. The raw material components of the facial cream in this comparative example, by weight, include:

[0172] 10 parts of active ingredients;

[0173] 70 parts of deionized water;

[0174] Glycerin 5 parts;

[0175] Xanthan gum 0.05~0.2 parts;

[0176] EDTA-2Na 0.05~0.1 parts;

[0177] Sodium hyaluronate 0.1-0.3 parts;

[0178] 1~2 parts of Camellia oleifera polypeptide;

[0179] Tween - 602.1 copies;

[0180] Span - 600.9 copies;

[0181] 3 parts white oil;

[0182] 3 parts of squalane;

[0183] DM1002 copies;

[0184] IPM 3 copies;

[0185] GCTT 4 copies;

[0186] 2 parts Vaseline;

[0187] Tocopherol 0.2 parts;

[0188] AHEG 0.6 parts;

[0189] EMT - 100.88 copies;

[0190] Among them, the effective ingredients include the following raw material extracts in parts by weight:

[0191] 30 parts of Pawpaw fruit extract;

[0192] 30 parts of Clematis extract;

[0193] 30 parts of Phellodendron chinense extract.

[0194] The preparation method of the repairing and anti-aging facial cream containing jasmine extract in this comparative example is the same as that in the above-mentioned Example 1.

[0195] Comparison test

[0196] (1) Anti-wrinkle efficacy test of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0197] Mouse fibroblasts were seeded into multi-well cell culture plates, with 0.8 × 10 5cells and starved them in serum-free medium for 24 hours. The starved cells were rinsed with PBS and treated with ultraviolet light (40 mJ). Then, 10 μg of the test sample was added to the cells twice within 48 hours. The MMP-1 isolated in the culture medium was measured using a kit (BIOTRAK, RPN2610). The strength of the anti-wrinkle effect was evaluated by calculating the expression inhibition rate of MMP-1, and the results are shown in Table 1. The calculation formula is as follows:

[0198] Inhibition rate = (AB) / A*100%

[0199] A: No test sample was added, but the expression of MMP-1 after UV irradiation

[0200] B: Expression of MMP-1 after adding test samples and UV irradiation

[0201] Table 1: Inhibition rate of MMP-1 expression by the creams prepared in Examples 1 to 3 and Comparative Example 1

[0202]

[0203] From the experimental data in Table 1, it can be seen that the inhibition rate of MMP-1 expression in Examples 1-3 is significantly higher than that in Comparative Example 1. Therefore, it can be known that the addition of jasmine extract can enhance the anti-wrinkle effect of the cream and inhibit the expression of MMP-1.

[0204] (2) Determination of the moisturizing properties of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0205] 1) Create a constant temperature and humidity environment (making a simple hygrostat)

[0206] The determination of the water loss rate of the moisturizer must be carried out in a constant temperature and humidity environment. The day before the experiment, place 50mL of saturated potassium carbonate solution at the bottom of the desiccator (the purpose is: the relative humidity of the saturated potassium carbonate solution is 43%, which is close to the relative humidity in the general environment, so it is used to simulate the relative humidity of the environment), so that the depth of the liquid is greater than 1cm but lower than the height of the porous partition, then place the porous partition, cover the lid of the desiccator, so that the entire system inside the desiccator is in a closed state, and place the desiccator in an environment of 25°C. After the partial pressure of water vapor in the air is constant, the relative humidity in the desiccator can be maintained at about 43%.

[0207] 2) Determination of product moisturizing properties

[0208] Wash a 3*7cm glass plate and put it in an oven to dry, then stick 3M medical tape on it (keep the tape clean and free of impurities). Weigh 0.2g of facial cream and apply it evenly on the 3M tape, the amount used is about 2mg / cm², and the weighed mass at this time is M1. Place the coated glass plate on the porous partition of a simple humidistat, and ensure air circulation above and below the partition. After leaving it for a period of time (2h, 4h, 6h, 8h), quickly take it out, and the weighed mass at this time is M2. Record the experimental data, conduct three parallel experiments, and take the average value.

[0209] 3) Determination of water loss rate by gravimetric method

[0210] Water loss rate (%) = (mass of sample before water loss M1 - mass of sample after water loss M2) / mass of sample before water loss M1

[0211] Table 2: Moisturizing efficacy test data of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0212]

[0213] It can be seen from the experimental data in Table 2 that the water loss rate of Examples 1-3 is lower than that of Comparative Example 1. Therefore, it can be known that the addition of jasmine extract can enhance the moisturizing effect of the cream.

[0214] (3) Determination of the repair function of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0215] 1) Sample processing

[0216] Weigh 0.1g of the sample and dissolve it in 100ml of deionized water. After stirring, mixing, and settling, the clarified solution is tested. Water-soluble gels and water-soluble gels can be dissolved directly; emulsifiers must be identified before dissolving. If they are emulsified (W / O) types, the dilution method should be used to convert them into emulsified (O / W) types. Then sterilize.

[0217] 2) MCT assay

[0218] 420 3dpf wild-type AB strain zebrafish were randomly selected and placed in a six-well plate. The tail fins of the zebrafish were cut off. Thirty zebrafish were treated in each well (experimental group) according to the volume concentrations of C1, C2, C3, C4, C5, C6, C7, C8, C9, C 10 , C 11 , C 12 , C 13 µL / mL water-dissolved and then sample solution was given to each well. A normal control group (zebrafish were treated with fish water) was set up at the same time. The volume of each well was 3mL. After 72 hours of sample solution treatment, the number of zebrafish deaths and toxicity in each experimental group were counted to determine the MTC of zebrafish to the sample solution.

[0219] 3) Evaluation of repair effect

[0220] 3dpf wild-type AB zebrafish were selected, and the entire tail fin was removed perpendicular to the trunk with a scalpel to establish a zebrafish tissue regeneration model. According to the above concentration exploration experiment, the MTC of zebrafish for the sample solution is CµL / mL. Therefore, the volume concentration of the sample solution repair effect evaluation experiment is set to: C 14 µL / mL (Example 1), C 15 µL / mL (Example 2), C 16 µL / mL (Example 3) and C 17 µL / mL (Comparative Example 1), a normal control group and a model control group were set up at the same time, among which C 14 =C 15 =C 16 =C 17 After the sample solution treatment, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. The images were saved and the zebrafish tail fin regeneration length (L) was statistically analyzed using Image-J advanced image processing software. The statistical analysis results of the tail fin regeneration length were used to evaluate the repair effect of the sample solution on the tissue. The statistical processing results were expressed as (mean ± standard error). The calculation formula for the sample solution repair rate is shown in formula (1).

[0221] (1)

[0222] Statistical analysis was performed using analysis of variance and Dunnett's T-test, and p < 0.05 indicated a significant difference.

[0223] Table 3: Repair rate data of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0224]

[0225] It can be seen from the experimental data in Table 3 that the repair rates of Examples 1-3 are significantly higher than that of Comparative Example 1. Therefore, it can be known that the addition of jasmine extract can enhance the repair effect of the cream.

[0226] (4) Determination of the Antioxidant Effect of the Creams Prepared in Examples 1 to 3 and Comparative Example 1

[0227] 1) Take 1 mg of DPPH in a beaker and dilute it to a 100 ml volumetric flask to prepare a 0.001 mg / ml DPPH solution. Weigh 1 g of each sample from Example 1, Example 2, Example 3 and Comparative Example 1 and dissolve them in 100 ml of deionized water. After stirring, mixing, and settling, the clarified solution is tested.

[0228] 2) A1 value measurement: Take 2 ml of DPPH solution and add it to a test tube, add X ml of sample solution, then add (YX) ml of sample solution solvent, mix, let stand for 30 min, and then measure the A1 value at a wavelength of 517 nm (X and Y are the amount of sample solution and the maximum amount of sample solution determined according to the preliminary experimental results, respectively).

[0229] 3) A2 value measurement: Take 2 ml of ethanol and add it to a test tube, add X ml of sample solution, then add (YX) ml of sample solution solvent, mix, let stand for 30 minutes, and measure the A2 value at a wavelength of 517 nm. (The solution preparation and measurement method are the same as the A1 value, replacing the DPPH solution with an ethanol solution to eliminate the effect of the sample solution's color change with concentration on the absorbance)

[0230] 4) A3 value measurement: Take 2 ml of DPPH solution and add it to a test tube, add Y ml of sample solution solvent, mix, let it stand for 30 minutes, and then measure the A3 value at a wavelength of 517 nm.

[0231] 5) Calculation of results:

[0232] Oxygen free radical scavenging rate calculation formula:

[0233]

[0234] 6) Plotting of sample concentration-clearance rate standard curve

[0235] According to the above measurement results, the sample concentration-clearance rate curve was drawn, the linear regression equation was fitted, and the half clearance rate (EC50) was calculated according to the equation as an indicator of antioxidant capacity. The results are shown in Table 4

[0236] Table 4: Free radical scavenging rate data of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0237]

[0238] It can be seen from the experimental data in Table 4 that the free radical scavenging rates of Examples 1-3 are significantly higher than that of Comparative Example 1. Therefore, it can be known that the addition of jasmine extract can enhance the antioxidant effect of the cream.

[0239] (5) Irritation test of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0240] The human trial test is used to observe the irritation of the sample to human skin. The adverse reactions of the subjects are evaluated at each visit time. The subjects are asked whether the test site has erythema, papules, dryness, desquamation, itching, tingling and other symptoms, and whether there is a new rash in the test site and whether the original symptoms are aggravated. In principle, the observation time should be one week, and the selection of subjects should meet the following points:

[0241] a) Select volunteers aged 18-60 who meet the test requirements as subjects;

[0242] b) Those with the following conditions cannot be selected as subjects;

[0243] c) Those who have used antihistamines in the past week or immunosuppressants in the past month; those who have used any anti-inflammatory drugs on the test site in the past two months; those who have inflammatory skin diseases that have not been cured clinically;

[0244] d) Patients with insulin-dependent diabetes mellitus; patients who are receiving treatment for asthma or other chronic respiratory diseases; patients who have received anti-cancer chemotherapy in the past 6 months; patients with immunodeficiency or autoimmune diseases; patients who have undergone bilateral mastectomy and bilateral axillary lymph node removal; breastfeeding or pregnant women;

[0245] e) Those who determine the test results due to scars, pigmentation, atrophy, port wine stains or other blemishes on the skin to be tested;

[0246] f) Participants in other clinical trial research; those with highly sensitive constitutions; non-voluntary participants or those who are unable to complete the required contents according to the trial requirements.

[0247] Table 5: Human skin adverse reaction levels

[0248]

[0249] Table 6: Irritation test results of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0250]

[0251] It can be seen from the experimental data in Table 4 that the creams prepared in Examples 1-3 and Comparative Example 1 are all non-irritating. And by comparing Examples 1-3 and Comparative Example 1, it can be seen that the jasmine extract has the advantage of being non-irritating.

[0252] (6) Determination of the whitening and spot-removing efficacy of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0253] 1) Solution preparation

[0254] PBS buffer: Take 38.0g of sodium dihydrogen phosphate and 5.04g of disodium hydrogen phosphate, add water to dissolve and dilute to 1000ml.

[0255] 1mol / L NaOH solution containing 10% dimethyl sulfoxide: Weigh 10g of dimethyl sulfoxide and dilute it to 100g with 1mol / L NaOH solution.

[0256] 2) Sample processing

[0257] Weigh 0.1000g of the sample accurately and dissolve it in 20ml of deionized water. After stirring, mixing, settling and other steps, the clarified solution is tested. Water-soluble gels and water-soluble gels can be dissolved directly; emulsifiers must be identified before dissolving. If they are emulsified (W / O) types, the transformation dilution method should be adopted to make them emulsified (O / W) types.

[0258] 3) Cell processing

[0259] The purchased B-16 melanocyte cell line was inoculated and cultured. When the cells grew to a sub-confluent state, they were digested with 0.25% trypsin for 2-3 minutes, the protease was discarded, and the cells were washed twice with sterile PBS buffer. 1640 culture medium was added and placed in a 37°C carbon dioxide incubator containing 5% CO2. Culture was carried out in a saturated humidity environment. Each experiment was taken from the same passage cells. 24 hours after the cells were inoculated, a part was replaced with a fresh culture medium with the sample solution added once as the sample group; the other part was replaced with a fresh culture medium once as the blank group. After incubation for 72 hours, the culture flask was placed under an inverted microscope to observe the changes in cell morphology and quantity, and the harvested cells were used for subsequent processing.

[0260] 4) Instrument reference conditions

[0261] The wavelength is 470mm.

[0262] 5) Experimental operation

[0263] Take the blank group cell solution and the sample group cell solution separately, add PBS buffer solution, and adjust the cell concentration to 10 3 / ml, take 1ml of cell suspension and place it in 3 centrifuge tubes as parallel samples, centrifuge (1000r / min, 5min) and discard the supernatant, first add 200ml of distilled water to resuspend the cells, then add 1ml of ethanol: ether = 1:1 (volume ratio) to dissolve the non-melanin opaque particles, and place at room temperature for 15min. Centrifuge (3000r / min, 5min) and discard the supernatant, add 1ml of 1mol / L NaOH solution containing 10% dimethyl sulfoxide by mass, place in 80℃ water bath for 30min, dissolve the cell mass completely, cool to room temperature, and obtain the blank group solution and sample group solution.

[0264] 6) Sample determination

[0265] The absorbance value D of the blank group solution and the sample group solution was measured at a wavelength of 470 nm using a spectrophotometer.

[0266] 7) Result calculation

[0267] Melanin content = D 样470 / / D 空470 ×100%

[0268] D 样470 is the absorbance value of the sample solution; D 空470 is the absorbance value of the blank control group solution.

[0269] Table 7: Melanin content data of the creams prepared in Examples 1 to 3 and Comparative Example 1

[0270]

[0271] It can be seen from the experimental data in Table 7 that the melanin content of Examples 1-3 is significantly lower than that of Comparative Example 1. Therefore, it can be known that the addition of jasmine extract can enhance the whitening and spot-lightening effect of the cream.

[0272] The above descriptions are only some embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Any equivalent device or equivalent process transformation made using the contents of the present invention specification, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A repairing and anti-aging facial cream containing jasmine extract, characterized in that: The raw material components thereof, measured by weight, include: 10~25 parts of active ingredients; 60~70 parts of deionized water; 4-6 parts of glycerin; Xanthan gum 0.05~0.2 parts; EDTA-2Na 0.05~0.1 parts; Sodium hyaluronate 0.1-0.3 parts; 1~2 parts of Camellia oleifera polypeptide; Tween-602~3 copies; Span - 600.5~1.5 copies; 2~4 parts of white oil; 2-4 parts of squalane; DM100 1~3 parts; IPM2~4 copies; GCTT 3~5 copies; 1~3 parts of Vaseline; Tocopherol 0.1~0.3 parts; AHEG 0.5~0.7 parts; EMT-100.5~1 copy; The functional ingredients are composed of the following raw material extracts in parts by weight: 40-50 parts of jasmine extract; 30-40 parts of Pawpaw fruit extract; 30-40 parts of Clematis chinensis extract; 30-40 parts of Psoralea corylifolia extract; The preparation method of the jasmine extract comprises: 5 to 15 parts of activated clay, 1 to 5 parts of activated carbon, 3 to 10 parts of diatomaceous earth and 1 to 5 parts of tourmaline powder are mixed and stirred to obtain a composite decolorizing agent, 15 to 30% of the composite decolorizing agent by volume is added to jasmine essential oil, and the mixture is stirred in a closed environment at 45 to 55° C. for 10 to 20 minutes, and the obtained solution is filtered to obtain a clarified essential oil, i.e., a jasmine extract; The preparation method of the Pawpaw tree fruit extract comprises: Weigh 100 g of Pawpaw fruit for drying, wash, dry, peel, and core the Pawpaw fruit, and beat it with a fruit crusher, then place it in a distillation tank, add deionized water at a solid-liquid ratio of 1:5, soak it at room temperature for 6 hours, perform reduced pressure steam distillation, heat the liquid for extraction for 5 hours, obtain an extract, then evaporate the water of the extract and concentrate it into a paste to obtain a Pawpaw fruit extract rich in chlorogenic acid; The preparation method of the Clematis chinensis extract comprises: Weigh 100 g of Clematis chinensis and dry it, add 300 mL of distilled water to the Clematis chinensis powder that has passed through a 20-mesh sieve, heat and reflux to obtain a brown-black liquid, take the extract and evaporate the water to obtain a brown viscous solid, and obtain the Clematis chinensis extract; The preparation method of the calendula officinalis extract comprises: The dried kelp was crushed into 80-100 meshes, 100 g of kelp powder was weighed, 300 mL of distilled water was added, and the mixture was heated under reflux to obtain an extract, and the extract was evaporated to remove water to obtain a viscous solid, thereby obtaining a kelp extract.

2. The repairing and anti-aging facial cream containing jasmine extract according to claim 1, characterized in that: The preparation method of the jasmine essential oil comprises: Pick jasmine flowers between 2 and 6 p.m., and keep them indoors for 2 to 4 hours between 6 and 12 a.m. at a temperature of 25 to 35°C, then wash and remove impurities from the jasmine flowers; Fresh jasmine flowers were freeze-dried, crushed through a 150-mesh sieve, and placed in an ultrasonic cell disruptor. Deionized water was then added according to a solid-liquid ratio of 1:3, and ultrasonic treatment was performed for 1 to 1.5 hours at a temperature not exceeding 30°C. The treated solution was added with cellulase and pectinase loaded with O-Fe3O4, and enzymolysis was carried out at 50°C and pH = 4.8 for 12 h; The solution was then loaded into a distillation tank, an appropriate amount of deionized water was added, and the distillation tank cover with a distillation tube was covered for reduced pressure steam distillation. The steam volume was set to 200 L / h, the vacuum degree was 13.33 kPa, and the extraction time was 2.5 h. After oil and water separation, anhydrous Na2SO4 was added to the separated oil layer to absorb water, and the mixture was left overnight. Finally, the solid Na2SO4 was filtered out to obtain jasmine essential oil.

3. The repairing and anti-aging facial cream containing jasmine extract according to claim 2, characterized in that: The preparation method of O-Fe3O4 loaded cellulase and pectinase comprises: 200mL nFe 3+ :nFe 2+ =1.5:1 mixed solution was placed in a three-necked flask, and 18 mL of 25%, v / v NH3 was quickly added at 70°C under N2 protection and high-speed stirring. After 1 min, 4.66 g of oleic acid was added dropwise; Maintain the reaction temperature and continue to stir rapidly for 1 h to obtain a black colloidal substance. Use a Nd-Fe-B magnet to separate the product from the reaction system, wash the product to neutrality, then add 160 mL, 10 mg / mL KMnO4 solution, ultrasonically shake for 8 h, filter and wash to neutrality, and vacuum dry for 40 h to obtain carboxylated Fe3O4 magnetic nanoparticles, labeled: O-Fe3O4; The O-Fe3O4 carrier was dispersed in 10 mL of cellulase and pectinase solution with a pH of 4.8, placed in a constant temperature water bath oscillator, and oscillated at 25°C and 150 r / min. Finally, the immobilized cellulase and pectinase were separated using a Nd-Fe-B magnet to obtain O-Fe3O4-loaded cellulase and pectinase.

4. The repairing and anti-aging facial cream containing jasmine extract according to claim 1, characterized in that: The preparation method of the camellia oleifera polypeptide comprises: Weigh 10g of defatted tea oil meal and crush it until it passes through an 80-mesh sieve; Add 200 mL of distilled water for soaking, and add 1 mol / L sodium hydroxide to adjust the pH to 8. Place in a 60°C water bath and heat for 80 min. Transfer to a centrifuge and centrifuge at 4000 r / min for 20 min. The supernatant was taken, 1 mol / L HCl was added to adjust the pH to 3 to the isoelectric point, and then centrifuged at 4000r / min for 20min, the upper liquid was discarded, and the lower material was the extracted camellia oleifera meal protein; finally, the camellia oleifera protein was obtained by freeze drying; Distilled water with a solid-liquid ratio of 1:25 was added to the extracted camellia oleifera protein, and then 1 mol / L HCl was added to adjust the pH to 10 and heated to 90°C for extraction for 20 minutes. Then 1 mol / L HCl was used to adjust the pH to 6.5, and papain was added. The enzymatic hydrolysis conditions were: temperature 54°C, enzyme addition amount 4U / g, and enzymatic hydrolysis time 4 hours; after enzymatic hydrolysis, the enzyme was inactivated by boiling water bath method for 30 minutes; then the protein was transferred to a centrifuge and centrifuged at 4000r / min for 15 minutes, and the supernatant was freeze-dried to obtain camellia oleifera polypeptide.

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