Raman spectroscopy measurement methods and equipment

Abstract

This application provides a Raman spectroscopy measurement method applied to a Raman spectroscopy measurement device. The Raman spectroscopy measurement device includes a photon output unit, a detection unit, a delay unit, and a photon collection unit. The Raman spectroscopy measurement method includes: outputting a pulsed light signal through the photon output unit, the pulsed light signal being used to illuminate the medium under test; monitoring the number of photons within a preset range around the photon collection unit through the detection unit; controlling the delay of the photon collection unit through the delay unit, the delay time being determined according to the number of photons; wherein the end time of the delay is later than the end time of the pulsed light signal; and, upon the end of the delay, activating the photon collection unit to collect the scattered photons from the medium under test. The technical solution of this application embodiment can filter interference and noise during the measurement process, thereby improving the accuracy and stability of the measurement results.

Description

Technical Field

[0001] This application relates to the field of optical measurement technology, and in particular to a Raman spectroscopy measurement method. Background Technology

[0002] With the development of biomedical testing technology, Raman spectroscopy is increasingly used in biochemical analysis. Raman spectroscopy is the scattering spectrum produced by the energy exchange between photons and analyte molecules after inelastic collisions, and it can reflect the characteristic information of the analyte molecules. Therefore, Raman spectroscopy can be used to analyze various components in bodily fluids. This measurement process does not require piercing the patient's skin and does not cause pain. Furthermore, Raman spectroscopy is faster than traditional biochemical analysis, providing results more quickly, which has significant advantages in fields such as emergency care and surgery.

[0003] However, in practical applications of Raman spectroscopy measurements, noise and interference in various measurement processes can introduce a lot of uncertainty into the measurement results, thereby reducing the accuracy of biochemical analysis.

[0004] It should be noted that the above content is not necessarily prior art, nor is it intended to limit the scope of patent protection of this application. Summary of the Invention

[0005] This application provides a Raman spectroscopy measurement method to solve or alleviate one or more of the technical problems mentioned above.

[0006] As one aspect of the embodiments of this application, this application provides a Raman spectroscopy measurement method applied to a Raman spectroscopy measurement device, the Raman spectroscopy measurement device including a photon output unit, a detection unit, a delay unit, and a photon collection unit, the Raman spectroscopy measurement method including:

[0007] The photon output unit outputs a pulsed light signal, which is used to illuminate the medium under test. The pulsed light signal passes sequentially through the first medium, the second medium, and the medium under test, and is scattered in different media to form first medium scattered photons, second medium scattered photons, and medium under test scattered photons, respectively.

[0008] The detection unit monitors the number of photons within a preset range around the photon collection unit.

[0009] The delay unit controls the delay of the photon collection unit, and the delay time is determined according to the number of photons; wherein the end time of the delay is later than the end time of the pulsed light signal.

[0010] When the delay ends, the photon collection unit is activated to collect the scattered photons from the medium under test.

[0011] Optionally, the Raman spectroscopy method further includes:

[0012] The delay unit controls the time window after the delay of the photon collection unit;

[0013] By adjusting the length of the time window, the photon collection unit is controlled to collect scattered photons from the test medium that meet the expected target.

[0014] Optionally, the Raman spectroscopy method further includes:

[0015] Repeat the following operations until a preset number of scattered photons from the test medium are obtained:

[0016] The pulsed optical signal is output to the medium under test;

[0017] Under the control of the delay unit, the photon collection unit collects the scattered photons generated by the pulse light signal when it arrives at the medium under test.

[0018] Optionally, the Raman spectroscopy measurement method further includes:

[0019] By adjusting the end time of the delay, the photon collection unit is controlled to collect scattered photons from the target medium under test; wherein, the scattered photons from the target medium under test are the scattered photons generated when the pulsed light signal reaches a specified depth in the medium under test.

[0020] Optionally, the Raman spectroscopy measuring device further includes a light source control unit, and the Raman spectroscopy measurement method includes:

[0021] The light source control unit sends pulse signals to the photon output unit to control the working time of the photon output unit;

[0022] The working time is the duration of the pulse signal.

[0023] Optionally, the test medium includes subcutaneous fluid at a specified depth.

[0024] Another aspect of this application provides a Raman spectroscopy measurement device, including:

[0025] A photon output unit is used to output a pulsed light signal; wherein the pulsed light signal is used to irradiate the medium under test through an intermediate medium;

[0026] Photon collection unit, used to collect photons;

[0027] The detection unit is used to monitor the number of photons within a preset range around the photon collection unit;

[0028] A delay unit is used to delay the time window for the photon collection unit to collect photons; wherein the delay time is determined according to the number of photons, and the end time of the delay is later than the end time of the pulsed light signal;

[0029] The delayed time window corresponds to the time when the scattered photons from the medium under test arrive at the photon collection unit, and the scattered photons from the medium under test are the scattered photons generated when the pulsed light signal arrives at the medium under test.

[0030] Optionally, the Raman spectroscopy measuring device further includes:

[0031] A light source control unit is used to control the operating time of the photon output unit via pulse signals;

[0032] The working time is the duration of the pulsed optical signal.

[0033] Optionally, the delay unit is further configured to:

[0034] The duration of the time window is adjusted to control the photon collection unit to collect photons scattered by the test medium that meet the expected target.

[0035] Optionally, the delay unit is further configured to:

[0036] Adjust the end time of the delay to control the photon collection unit to collect scattered photons from the target medium under test;

[0037] The scattered photons of the target medium are the scattered photons generated when the pulsed light signal reaches a specified depth in the medium.

[0038] The embodiments of this application employing the above-described technical solution may have the following advantages:

[0039] A pulsed light signal is emitted through a photon output unit, and the pulsed light signal passes through multiple intermediate media to reach the test medium. During this process, photons are scattered in different media layers, forming intermediate medium-scattered photons and test medium-scattered photons. A detection unit monitors the number of photons near the photon collection unit in real time, and a delay unit controls the delay of the photon collection unit. When the number of photons drops to a certain threshold, the delay ends and the photon collection unit is activated, ensuring that it primarily collects photons from the test medium. This embodiment utilizes a delay unit to control the delayed activation of the photon collection unit. Since the scattered photons from the intermediate media essentially disappear within the delay time, the photon collection unit primarily collects photons scattered from the test medium, filtering out interference and noise during the measurement process, thereby improving the accuracy and stability of the measurement results. Attached Figure Description

[0040] In the accompanying drawings, unless otherwise specified, the same reference numerals throughout the various drawings denote the same or similar parts or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings depict only some embodiments disclosed in this application and should not be construed as limiting the scope of this application.

[0041] Figure 1 This is a block diagram of the Raman spectroscopy measurement device provided in the embodiments of this application;

[0042] Figure 2 A flowchart illustrating a Raman spectroscopy measurement method according to Embodiment 1 of this application is shown schematically.

[0043] Figure 3 The illustration schematically shows the new procedure of the Raman spectroscopy measurement method according to Embodiment 1 of this application;

[0044] Figure 4 The illustration schematically shows the new procedure of the Raman spectroscopy measurement method according to Embodiment 1 of this application;

[0045] Figure 5 The illustration schematically shows the new procedure of the Raman spectroscopy measurement method according to Embodiment 1 of this application;

[0046] Figures 6A-6B This is a schematic diagram illustrating an application example of the Raman spectroscopy measurement method of Embodiment 1 of this application. Detailed Implementation

[0047] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other. The application will now be described in detail with reference to the accompanying drawings and embodiments.

[0048] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such terms can be used interchangeably where appropriate so that the embodiments of this application described herein can be implemented, for example, in orders other than those illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover a non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0049] The following provides a definition of the terminology used in this application.

[0050] Light scattering refers to the phenomenon where light deviates from its original direction when passing through an inhomogeneous medium. When light shines on the surface of an object, two types of scattering occur: Rayleigh scattering and Raman scattering. The vast majority of photons (approximately 99.999%) undergo Rayleigh scattering, while only a tiny minority, about one in ten million, undergo Raman scattering.

[0051] Raman scattering: an inelastic scattering phenomenon of photons. Inelastic collisions occur between photons and molecules / atoms in the medium, resulting in energy exchange. After scattering, the wavelength, frequency, and energy of the photons change.

[0052] Rayleigh scattering: a type of elastic scattering of photons. Photons undergo elastic collisions with molecules / atoms in the medium, and their energy remains unchanged before and after the collision. Consequently, the wavelength, frequency, and energy of the photons do not change after scattering.

[0053] Raman spectroscopy: a type of scattering spectroscopy based on the interaction between light and material molecules, applicable to molecular analysis. Analysis of Raman spectra can yield information such as molecular vibrations and molecular structure.

[0054] Light path: refers to the path of light propagation, including refraction and reflection during light propagation.

[0055] Transmittance: The ratio of transmitted luminous flux to incident luminous flux, used to indicate the degree of light transmission of a transparent object, i.e., characterizing the light-transmitting property of an object. Transmission is the phenomenon of incident light exiting an object after refraction. The object through which light is transmitted is transparent or translucent, such as glass, color filters, etc. If the transparent object is colorless, most light passes through the object, except for a small amount of light that is reflected.

[0056] To facilitate understanding of the technical solutions provided in the embodiments of this application by those skilled in the art, the relevant technologies are described below:

[0057] Raman spectroscopy is a scattering spectrum produced by inelastic collisions between photons and molecules, which can reflect the characteristic information of molecules. Therefore, Raman spectroscopy can be applied to biochemical analysis, such as using Raman spectroscopy to analyze various components in human body fluids. The advantages of Raman spectroscopy are: (1) It does not require piercing the patient's skin and will not cause the patient pain. (2) It is fast, and the measurement results can be obtained within one minute. (3) Compared with the traditional biochemical analysis process, Raman spectroscopy analysis can obtain analytical results much faster. Therefore, Raman spectroscopy has great advantages in fields such as emergency care and surgery, and can even achieve real-time analysis.

[0058] However, the applicant has learned that in the actual application of Raman spectroscopy analysis, the Raman spectroscopy measurement process is subject to various noises and interferences. These noises and interferences introduce a lot of uncertainty into the measurement results, resulting in a low signal-to-noise ratio in the Raman spectroscopy measurement results, which in turn affects the accuracy of biochemical analysis.

[0059] Therefore, this application provides a technical solution for Raman spectroscopy measurement. In this technical solution, (1) various interferences during the measurement process can be filtered out, and the actual signal to be measured can be separated, thereby improving the accuracy and stability of the measurement results. (2) Raman spectra of subcutaneous fluid layers at different depths can be measured, facilitating biochemical analysis of subcutaneous fluids at different depths. See below for details.

[0060] Exemplary embodiments according to this application will now be described in more detail with reference to the accompanying drawings. It should be understood that these exemplary embodiments may be implemented in many different forms and should not be construed as being limited to the embodiments set forth herein.

[0061] The Raman spectroscopy measurement method provided in this application embodiment can be applied to Raman spectroscopy measurement equipment.

[0062] like Figure 1 As shown, the Raman spectroscopy measurement device 100 may include a central control unit 1000, a light source control unit 1100, a photon output unit 1110, a detection unit 1200, a delay unit 1300, and a photon collection unit 1310. Wherein:

[0063] The main control unit 1000 can be electrically connected to the light source control unit 1100, the photon output unit 1110, the detection unit 1200, the delay unit 1300, and the photon collection unit 1310, and is configured to send various control signals to different units to control the start-up and shutdown of different units.

[0064] The light source control unit 1100 is used to control the switching of the photon output unit.

[0065] The photonic output unit 1110 is used to output optical signals. The photonic output unit may include a laser or the like for generating a light beam.

[0066] The detection unit 1200 is used to detect the number of photons near the photon collection unit.

[0067] The delay unit 1300 is used to control the delayed start-up of the photon collection unit.

[0068] Photon collection unit 1310 is used to collect photons.

[0069] The Raman spectroscopy measurement device described in this application embodiment can have a variety of applications, such as acquiring subcutaneous fluid information of the human body.

[0070] The technical solution of this application will be described below through multiple embodiments using a Raman spectroscopy measurement device as the main implementation. It should be understood that these embodiments can be implemented in many different forms and should not be construed as being limited to the embodiments described herein.

[0071] Example 1

[0072] Figure 2 A flowchart illustrating a Raman spectroscopy measurement method according to Embodiment 1 of this application is shown schematically.

[0073] like Figure 2 As shown, this Raman spectroscopy measurement method may include:

[0074] Step S200: A pulsed light signal is output through the photon output unit, and the pulsed light signal is used to illuminate the medium under test; wherein, the pulsed light signal passes through the first medium, the second medium and the medium under test in sequence, and is scattered in different media to form first medium scattered photons, second medium scattered photons and medium under test scattered photons respectively.

[0075] Step S202: Monitor the number of photons within a preset range around the photon collection unit using the detection unit.

[0076] Step S204: Control the delay of the photon collection unit through the delay unit, wherein the delay time is determined according to the number of photons; wherein the end time of the delay is later than the end time of the pulse light signal.

[0077] Step S206: When the delay ends, the photon collection unit is activated to collect the scattered photons from the medium under test.

[0078] In this embodiment, a pulsed light signal can be output through a photon output unit, and the pulsed light signal is used to irradiate the test medium. In an exemplary application, since it is necessary to measure subcutaneous fluid, the pulsed light signal inevitably needs to pass through multiple intermediate media before reaching the test medium. Therefore, after being emitted, the pulsed light signal will sequentially pass through a first medium (air), a second medium (skin surface), and then reach the test medium (subcutaneous fluid). A specific example of the process is as follows:

[0079] (1) Some photons are scattered and reflected by dust in the air to form first medium scattered photons. The first scattered photons reach the photon collection unit after several scattering and reflections.

[0080] (2) Some photons reach the skin surface and are scattered and reflected, forming second-medium scattered photons. After several scattering and reflections, the second-medium scattered photons then reach the photon collection unit.

[0081] (3) Some photons penetrate the skin surface and go deep into the subcutaneous layer. When they encounter various subcutaneous components (such as body fluids and tissue fluids), they will be scattered and reflected, forming photons scattered by the test medium. After several scattering and reflections, the photons scattered by the test medium finally reach the photon collection unit.

[0082] In this process, due to the barrier function of the skin, the skin surface acts as the primary source of reflection. Most photons are scattered and reflected when they reach the skin surface. However, the skin surface of different people varies greatly due to various reasons, including: (1) natural skin color; (2) differences in age, amount of dead skin, etc.; and (3) differences in the skin surface caused by the growth environment. These differences in the skin surface lead to different reflection characteristics. Therefore, when performing Raman spectroscopy measurements, the information on subcutaneous tissue fluid / body fluid that actually needs to be obtained will be severely interfered with due to the differences in the characteristic information of the skin surface, resulting in a very large uncertainty in the measurement results.

[0083] In this embodiment, to obtain accurate information about subcutaneous tissue fluid, the number of photons within a preset range around the photon collection unit can be monitored in real time by a detection unit, and the photon collection unit can be delayed and activated by a delay unit. The delay time can be determined based on changes in the number of photons, and the end time of the delay is later than the end time of the pulsed light signal. For example, when the number of photons drops to a preset threshold, the delay can be ended and the photon collection unit can be activated. Since the photons scattered by the first and second media have essentially disappeared within the delay time, the photon collection unit primarily collects photons scattered by the subcutaneous body fluid after activation.

[0084] In this embodiment, the delay time can be determined by real-time monitoring of the change in the number of photons near the photon collection unit through the detection unit, and the delay unit can be used to control the delayed start of the photon collection unit. This can effectively filter out interference and noise in the measurement process, obtain real subcutaneous fluid scattered photons, and thus improve the accuracy and stability of the measurement results.

[0085] In some embodiments, the time required for the optical path can be calculated in advance based on theory to determine the theoretical range of the delay time. For example, if a distance of 1mm under the skin needs to be detected, the calculated optical path is 2mm, and the speed of light in water is 225,000 km / s, so the theoretical delay can be 8.89 ps. Since differences in the delay circuits of different devices may lead to inconsistencies in the delay, the delay time can be determined and adjusted based on the predetermined theoretical range and the number of photons monitored in real time to accommodate the differences between different measurement devices and to optimize the delay time in real time. In other embodiments, the delay time can also be adjusted according to the minimum step length that the chip can adjust.

[0086] In some embodiments, photon receivers can be placed at pre-set detection points. These detection points can be positioned around the photon collecting unit to monitor the number of photons around the unit. The photon receivers can be selected from standard reflective materials with low transmittance. The number of photons received by the photon receivers is monitored synchronously by the detection unit. When the number of photons received by the photon receivers decreases to a certain threshold, indicating that the scattered photons from the skin surface and airborne dust have essentially disappeared, the delay can be terminated. Monitoring the number of photons around the photon collecting unit using photon receivers allows for rapid determination and adjustment of the delay time.

[0087] In optional embodiments, such as Figure 3 As shown, this Raman spectroscopy measurement method may further include:

[0088] Step S300: Control the time window after the delay of the photon collection unit through the delay unit.

[0089] Step S302: By adjusting the time window, the photon collection unit is controlled to collect photons scattered by the test medium that meet the expected target.

[0090] In this embodiment, the photon collection unit can be switched on and off via a delay unit. The delay unit can send a pulse control signal to the photon collection unit. The photon collection unit starts upon receiving the pulse control signal and shuts down when the pulse control signal ends. The photon collection unit can collect photons within this time window. By adjusting the length of the time window, the photon collection unit can collect photons scattered by the test medium that meet the expected target. For example, it can collect photons scattered by the test medium from 1mm-3mm under the skin, or from 1mm-5mm under the skin. Adjusting the time window makes the measurement process flexible and controllable, facilitating the acquisition of the truly needed subcutaneous information.

[0091] In an optional embodiment, the Raman spectroscopy measurement method may further include: repeating the following operations until a preset number of photons scattered by the test medium are obtained: outputting the pulsed light signal to the test medium; and, under the control of the delay unit, collecting the photons scattered by the test medium generated when the pulsed light signal reaches the test medium through the photon collection unit.

[0092] On the one hand, when light signals illuminate a medium, the vast majority of photons undergo Rayleigh scattering, with only a tiny fraction (one in ten million) undergoing Raman scattering, resulting in a very small number of detectable Raman-scattered photons. On the other hand, because human skin generates heat from photon absorption, the intensity of the light signal cannot be infinitely high, otherwise it would burn the skin, further limiting the number of detectable Raman-scattered photons. After a single light signal emission, the number of Raman-scattered photons collected by the photon collection unit is relatively small, making it difficult to meet the requirements of Raman spectroscopy measurements.

[0093] In this embodiment, to collect a sufficient number of Raman scattered photons, the Raman spectroscopy measurement process can be repeated multiple times. This allows the photon collection unit to repeatedly collect the scattered photons generated by the light signal reaching the test medium. Multiple measurements can collect a sufficient number of Raman scattered photons, effectively improving the reliability and accuracy of Raman spectroscopy measurements.

[0094] In optional embodiments, such as Figure 4 As shown, this Raman spectroscopy measurement method may further include:

[0095] Step S400: By adjusting the end time of the delay, the photon collection unit is controlled to collect the scattered photons of the target medium under test; wherein, the scattered photons of the target medium under test are the scattered photons generated when the pulse light signal reaches a specified depth in the medium under test.

[0096] In this embodiment, the delay end time can be adjusted, i.e., the activation time of the photon collection unit can be adjusted. For example, the delay ends when the number of photons drops to a second threshold. The value of the second threshold can be determined based on the optical path time of 1 mm subcutaneously. When the number of photons drops to the first threshold, it indicates that the photons scattered by the first and second media have essentially disappeared. When the number of photons drops to the second threshold, it can be determined that the scattered photons 1 mm subcutaneously have also essentially disappeared. At this point, the delay ends and the photon collection unit is activated. The photon collection unit mainly collects scattered photons from the test medium deeper than 1 mm subcutaneously. It should be noted that the second threshold can be adjusted according to the actual specified subcutaneous depth for measurement, and is not limited here.

[0097] In this embodiment, by adjusting the delay time, interference from scattered photons from the test medium at depths other than the target depth can be reduced. The system primarily collects scattered photons generated by the test medium at a specified depth, thereby measuring subcutaneous fluid layers at different depths. Filtering out various interferences during the measurement process and acquiring the signals that are truly needed for measurement also improves the accuracy and stability of the measurement results.

[0098] In optional embodiments, such as Figure 5 As shown, this Raman spectroscopy measurement method may further include:

[0099] Step S500: The light source control unit sends a pulse signal to the photon output unit to control the working time of the photon output unit; wherein the working time is the duration of the pulse signal.

[0100] In this embodiment, a pulse signal can be generated by the light source control unit and sent to the photonic output unit. Upon receiving the pulse signal, the photonic output unit turns on and outputs a pulsed light signal. After the pulse signal ends, the photonic output unit turns off and cuts off the pulsed light signal output. Thus, the operating time of the photonic output unit is the duration of the pulse signal. By controlling the switching of the photonic output unit using a pulse signal, the operating time of the photonic output unit can be precisely adjusted.

[0101] In an optional embodiment, the test medium includes subcutaneous fluid at a specified depth. Therefore, Raman spectroscopy can be used to measure subcutaneous fluid at a specified depth, such as 1 mm or 2 mm, to quickly obtain the required characteristic information of the subcutaneous fluid and improve the speed and accuracy of biochemical analysis.

[0102] To make the embodiments of this application easier to understand, the following are combined with... Figures 6A-6B An exemplary application is provided. In this exemplary application, the Raman spectroscopy measurement method can be applied to a Raman spectroscopy measurement device. The Raman spectroscopy measurement device may include a photon output unit, a detection unit, a delay unit, a photon collection unit, and a light source control unit.

[0103] S11, the light source control unit generates a short pulse and sends it to the photon output unit.

[0104] S12, the photon output unit turns on and outputs a pulsed light signal when it receives a short pulse, and turns off the pulsed light signal output when the short pulse ends.

[0105] The pulsed light signal undergoes the following processes after being emitted:

[0106] (1) When photons enter the air, they are scattered and reflected when they encounter dust. After several scattering and reflections, they reach the photon collection unit. During the T0 stage, the number of photons near the photon collection unit gradually increases.

[0107] (2) Photons reach the skin surface and are scattered and reflected, forming scattered photons on the skin surface. After several scattering and reflections, they then reach the photon collection unit. In stage T1, the number of photons near the photon collection unit gradually reaches a stable level and remains stable.

[0108] (3) Some photons penetrate the skin surface and go deep into the subcutaneous layer. When they encounter various components in the subcutaneous layer (such as body fluids and tissue fluids), they are scattered and reflected, forming photons scattered by the test medium. After several scattering and reflections, the photons scattered by the test medium finally reach the photon collection unit.

[0109] S13, the detection unit monitors the number of photons within a preset range around the photon collection unit.

[0110] S14, the delay unit controls the delay of the photon collection unit.

[0111] The delay corresponds to stage T2, and the photon collection unit remains off during stages T0, T1, and T2.

[0112] S15, when the number of photons drops to the first threshold, the delay unit ends the delay and sends a control pulse to the photon collection unit.

[0113] S16, the photon collection unit starts and collects photons when it receives a control pulse, and stops collecting photons when the control pulse ends.

[0114] In this process, the photon collection unit receives scattered photons from under the skin through a receiving window, which corresponds to stage T3.

[0115] S17. Repeat steps S11-S16 until a preset number of scattered photons from the test medium are collected.

[0116] Repeat the Raman spectroscopy measurement process, allowing the photon collection unit to work repeatedly for a sufficient number of T3 stages to collect enough photons.

[0117] Compared to the Raman spectroscopy measurement methods known to the applicant, the Raman spectroscopy measurement method provided in this application can filter out various interferences during the measurement process, separate the signal that actually needs to be measured, improve the accuracy and stability of the measurement results, and can measure the Raman spectra of subcutaneous fluid layers at different depths, which facilitates biochemical analysis of subcutaneous fluids at different depths.

[0118] Example 2

[0119] The embodiments of this application also provide the following technical solutions, and specific technical details can be found above.

[0120] A Raman spectroscopy measuring device 100 includes:

[0121] The photon output unit 1110 is used to output a pulsed light signal; wherein the pulsed light signal is used to irradiate the medium under test through an intermediate medium;

[0122] Photon collection unit 1310 is used to collect photons;

[0123] The detection unit 1200 is used to monitor the number of photons within a preset range around the photon collection unit 1310;

[0124] The delay unit 1300 is used to delay the time window for the photon collection unit 1310 to collect photons; wherein the delay time is determined according to the number of photons, and the end time of the delay is later than the end time of the pulse light signal;

[0125] The delayed time window corresponds to the time when the scattered photons from the medium under test arrive at the photon collection unit 1310, and the scattered photons from the medium under test are the scattered photons generated when the pulse light signal arrives at the medium under test.

[0126] In an optional embodiment, the Raman spectroscopy measuring device may further include a light source control unit 1100;

[0127] The light source control unit 1100 is used to control the working time of the photon output unit 1310 through a pulse signal; wherein the working time is the duration of the pulse light signal.

[0128] In an optional embodiment, the delay unit 1300 is further configured to:

[0129] The time window length is adjusted to control the photon collection unit 1310 to collect photons scattered by the test medium that meet the expected target.

[0130] In an optional embodiment, the delay unit 1300 is further configured to:

[0131] Adjust the end time of the delay to control the photon collection unit 1310 to collect scattered photons from the target medium under test; wherein, the scattered photons from the target medium under test are the scattered photons generated when the pulsed light signal reaches a specified depth in the medium under test.

[0132] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0133] Unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0134] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps described in these embodiments do not limit the scope of this application. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.

[0135] It should also be noted that the terms "one embodiment," "another embodiment," or "embodiment" used in this specification refer to specific features, structures, or characteristics described in connection with that embodiment, which are included in at least one embodiment described in the general description of this application. The appearance of the same expression in multiple places in the specification does not necessarily refer to the same embodiment. Furthermore, when a specific feature, structure, or characteristic is described in connection with any embodiment, the intention is to suggest that implementing such a feature, structure, or characteristic in conjunction with other embodiments also falls within the scope of this application.

[0136] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0137] It should also be noted that the above are merely preferred embodiments of this application and do not limit the scope of patent protection of this application. Any equivalent structural or procedural changes made using the content of this application’s specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the scope of patent protection of this application.

Claims

1. A Raman spectroscopy measurement method characterized by, Applied to a Raman spectrum measuring device, the Raman spectrum measuring device comprising a photon output unit, a detection unit, a delay unit and a photon collection unit, the method comprising: outputting a pulsed light signal through the photon output unit, the pulsed light signal being used to irradiate a medium to be measured; wherein the pulsed light signal sequentially passes through a first medium, a second medium and the medium to be measured, and scatters in different media to form first medium scattered photons, second medium scattered photons and medium to be measured scattered photons respectively; monitoring the number of photons within a preset range around the photon collection unit through the detection unit; controlling the delay of the photon collection unit through the delay unit, the delay time being determined according to the number of photons; wherein the end time of the delay is later than the end time of the pulsed light signal; in the case that the delay ends, starting the photon collection unit to collect the medium to be measured scattered photons; wherein the method further comprises: controlling the photon collection unit to collect target medium to be measured scattered photons by adjusting the end time of the delay; wherein the target medium to be measured scattered photons are scattered photons generated when the pulsed light signal reaches a specified depth in the medium to be measured.

2. The Raman spectroscopy measurement method according to claim 1, wherein The method further comprises: controlling the time window of the photon collection unit after the delay through the delay unit; controlling the photon collection unit to collect medium to be measured scattered photons meeting the expected target by adjusting the time length of the time window.

3. The Raman spectroscopy measurement method according to claim 1, wherein The method further comprises: repeating the following operations until a preset number of medium to be measured scattered photons are obtained: outputting the pulsed light signal to the medium to be measured; collecting the medium to be measured scattered photons generated when the pulsed light signal reaches the medium to be measured through the photon collection unit under the control of the delay unit.

4. The Raman spectrum measuring method according to claim 1, the Raman spectrum measuring apparatus further comprising a light source control unit, characterized by, The method comprises: sending a pulsed signal to the photon output unit through the light source control unit to control the working time of the photon output unit; wherein the working time is the duration of the pulsed signal.

5. The Raman spectroscopy measurement method according to any one of claims 1 to 4, characterized by, The medium to be measured includes subcutaneous body fluid at a specified depth.

6. A Raman spectroscopic measuring apparatus characterized by comprising: Comprising: a photon output unit for outputting a pulsed light signal; wherein the pulsed light signal is used to irradiate a medium to be measured through an intermediate medium; a photon collection unit for collecting photons; a detection unit for monitoring the number of photons within a preset range around the photon collection unit; a delay unit for delaying the time window of the photon collection unit for collecting photons; wherein the delay time is determined according to the number of photons, and the end time of the delay is later than the end time of the pulsed light signal; wherein the time window after the delay corresponds to the time when the medium to be measured scattered photons reach the photon collection unit, the medium to be measured scattered photons being scattered photons generated when the pulsed light signal reaches the medium to be measured; wherein the delay unit is further used to adjust the end time of the delay to control the photon collection unit to collect target medium to be measured scattered photons; wherein the target medium to be measured scattered photons are scattered photons generated when the pulsed light signal reaches a specified depth in the medium to be measured.

7. The Raman spectroscopy measuring apparatus according to claim 6, characterized by Further comprising: A light source control unit is configured to control the working time of the photon output unit by a pulse signal. The working time is the duration of the pulse light signal.

8. The Raman spectroscopy measuring apparatus according to claim 6, characterized by The delay unit is further configured to: adjust the time length of the time window, so as to control the photon collection unit to collect the scattered photons of the target medium.

9. The Raman spectroscopy measuring apparatus according to claim 6, characterized by, The delay unit is further configured to: adjust the end time of the delay, so as to control the photon collection unit to collect the scattered photons of the target medium. The scattered photons of the target medium are the scattered photons generated when the pulse light signal reaches a specified depth in the target medium.

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