Method and device for preparing umbilical cord segment and application thereof

Abstract

A method for preparing an umbilical cord segment, firstly, the ex vivo umbilical cord is placed into the container from the opening at one end, passes through the blade of a knife body, and stops at the bottom surface of the other end of the container; under the action of external force, the blade of the knife body moves from the container cavity toward the notch on the container side wall, and gradually approaches the outer wall of the container cavity, and cuts the umbilical cord in the container cavity; at the same time, under the action of the knife body, the cover body covering the bottom surface of the other end of the container and connected to the knife body opens, exposing the opening at the other end of the container, so that the cut umbilical cord segment is discharged from the opening at the other end of the container; when the external force is removed, the blade of the knife body returns to the container cavity; at the same time, the cover body connected to the knife body closes the opening at the other end of the container and covers the bottom surface of the other end of the container. The method provided by the present invention can obtain umbilical cord segments of uniform length, with a flat cut surface, and the position of Wharton's jelly is easy to identify, and is also conducive to the standardization of the preparation of Wharton's jelly and umbilical cord mesenchymal stem cells.

Description

[0001] The present invention is a divisional application filed based on the original application with the application date of September 2, 2019, the application number of 201910830780.5, and the invention name of “Method and device for preparing umbilical cord segments and their application”. Technical Field

[0002] The invention relates to a method for preparing biomaterials, in particular to a method and device for shearing an umbilical cord to prepare umbilical cord segments, as well as an application in separating and preparing Wharton's jelly in umbilical cord tissue and preparing umbilical cord mesenchymal stem cells. Background Art

[0003] The umbilical cord is the connecting structure between the fetus and the placenta. It is shaped like a rope, with a smooth and transparent surface, and contains connective tissue, an umbilical vein and a pair of umbilical arteries. Umbilical cord blood stem cells can be isolated from the blood contained in the umbilical cord of newborns, and umbilical cord mesenchymal stem cells can be isolated from the connective tissue.

[0004] In the process of preparing umbilical cord mesenchymal stem cells, the whole umbilical cord must be cut into small pieces of about 1cm to 3cm in length, and then the Wharton's Jelly is peeled off from it. The peeling is usually done by cutting the umbilical cord along the umbilical vein, and after unfolding, the umbilical vein and two umbilical arteries are scraped off with a scalpel, and then the outermost membrane of the umbilical cord is removed with a scalpel. The remaining white jelly-like substance is the Wharton's Jelly. Then the Wharton's Jelly can be cut into pieces and digested to separate the umbilical cord mesenchymal stem cells.

[0005] At present, the operation of cutting the umbilical cord into small pieces is carried out in an open space with forceps and scissors. Since the umbilical cord is long and has a hard / slippery texture, the existing method is not only difficult to operate, but also easy to slip. It is laborious to clamp the umbilical cord with forceps, and the lengths of the obtained umbilical cord segments are different and the cut surface is uneven, which makes it difficult to expose the position of the Wharton's jelly, making it difficult to remove the Wharton's jelly in the subsequent operation, and the implementation efficiency is low.

[0006] Utility model patent 201320295292.7 discloses an umbilical cord clamp, which has the characteristics of large clamping surface, firm clamping, not easy to slip and easy cutting operation. It includes a base and a pressure cover, which are fixed by a rotating shaft. A square groove is provided on the upper side of the base, and an elliptical column is provided on the lower side of the pressure cover. The long axis of the elliptical column is consistent with the length direction of the pressure cover. The width of the square groove is greater than the long axis of the elliptical column. Positioning holes are provided at both ends of the base, and positioning devices are provided at both ends of the pressure cover.

[0007] Utility model patent 201621090405.X discloses an umbilical cord tissue shearing device, the blade part is folded upward at a certain angle, which is more in line with the characteristics of human mechanics, improves the convenience of use, and increases the number of blades, greatly improving the shearing efficiency. It includes two hinged handles, the upper ends of the handles are respectively provided with mutually matching front and back blade heads, the front part of the blade head is provided with a blade part folded upward at a certain angle, and the front and back blade heads are respectively provided with at least two blade parts.

[0008] The prior art has proposed several technical solutions for clamping and cutting the umbilical cord, but similar to the method of forceps and scissors, they are usually implemented by two instruments, which are relatively independent and lack coordination in operation. Summary of the invention

[0009] One object of the present invention is to provide a method for preparing an umbilical cord segment, so that the position of Wharton's jelly in the umbilical cord is easy to identify, which is beneficial to the subsequent stripping operation of the Wharton's jelly.

[0010] Another object of the present invention is to provide a device for preparing umbilical cord segments, which can complete the clamping and cutting of the umbilical cord in one instrument to obtain umbilical cord segments of uniform length, which is beneficial to the subsequent stripping operation of Wharton's jelly.

[0011] Another object of the present invention is to provide a device for preparing umbilical cord segments, so that the position of Wharton's jelly in the umbilical cord is easy to identify, which is beneficial to the subsequent stripping operation of Wharton's jelly.

[0012] Another object of the present invention is to provide a method for preparing umbilical cord segments, and the application of the method in the separation and preparation of Wharton's jelly, and the preparation of umbilical cord mesenchymal stem cells.

[0013] Another object of the present invention is to provide a device for preparing umbilical cord segments, and its application in the separation and preparation of Wharton's jelly and the preparation of umbilical cord mesenchymal stem cells.

[0014] The present invention provides a method for preparing an umbilical cord segment, comprising:

[0015] First, the ex vivo umbilical cord is inserted from the opening at one end of the container, passes through the blade of the knife body, and stops at the bottom surface of the other end of the container;

[0016] Then, under the action of external force, the blade of the knife body moves from the container cavity toward the notch on the container side wall, and gradually approaches the outer wall of the container cavity to cut the umbilical cord in the container cavity; at the same time,

[0017] Under the force of the knife body, the cover body covering the bottom surface of the other end of the container and connected to the knife body is opened to expose the opening of the other end of the container, so that the cut umbilical cord segment is discharged from the opening of the other end of the container;

[0018] When the external force is removed, the blade of the knife body returns to the container cavity; at the same time,

[0019] The cover body connected with the knife body closes the opening at the other end of the container and covers the bottom surface of the other end of the container.

[0020] By adopting the method provided by the present invention, the cut umbilical cord segment has a smooth cut surface, and the position of Wharton's jelly is easily determined, and then the Wharton's jelly is peeled off and obtained, and umbilical cord mesenchymal stem cells are further prepared.

[0021] By applying intermittent force to the handle of the knife body, the umbilical cord can be continuously sheared and automatically sheared. The umbilical cord segments obtained are uniform in length and have a smooth cut surface, making it easy to determine the position of Wharton's jelly, which significantly improves the efficiency of Wharton's jelly preparation and is conducive to establishing standardized operating specifications for the preparation of umbilical cord mesenchymal stem cells, thereby achieving standardization of the preparation of Wharton's jelly and umbilical cord mesenchymal stem cells.

[0022] In order to complete the clamping and cutting of the umbilical cord in one device, to obtain umbilical cord segments of uniform length, the present invention also provides a device for preparing umbilical cord segments, comprising:

[0023] A first container, with both ends opened, for accommodating the umbilical cord;

[0024] A second container is placed below the first container and includes a first opening and a second opening, wherein the first opening is connected to the opening of the first container, an upper end surface of the first opening is not in contact with a lower end surface of the first container, and a shear space is provided therebetween;

[0025] A first blade body, comprising a first blade portion, a first connecting portion and a first handle portion, wherein the first blade portion is disposed in the shearing space, the first handle portion is disposed outside the side wall of the first container or outside the side wall of the second container, and the first connecting portion is used to drive the first blade portion to move in the shearing space;

[0026] The second blade body comprises a second blade portion, a second connecting portion and a second handle portion, wherein the second blade portion is arranged in the shearing space, the second handle portion is arranged outside the side wall of the first container or outside the side wall of the second container, and the second connecting portion is used to drive the second blade portion to move in the shearing space;

[0027] The cover body includes a main body and a connecting rod. The main body is covered on the second opening to close and open the second opening. One end of the connecting rod is arranged on the main body, and a force is applied to the other end to displace the main body to open the second opening. When the force is removed, the main body is reset to close the second opening.

[0028] Another specific embodiment of the device for preparing umbilical cord segments also includes an elastic mechanism, whose two ends respectively abut against the outer wall of the second container and the connecting rod, which undergoes elastic deformation under the action of external force, recovers after the force is removed, and resets the body to close the second opening.

[0029] Another specific embodiment of the device for making umbilical cord segments also includes a first supporting member, which is respectively connected to the connecting rod and the first handle to achieve the connection between the first knife body and the cover body. After the force is removed, the moved first blade is reset and the body is reset to close the second opening.

[0030] Another specific embodiment of the device for making umbilical cord segments also includes a second supporting member, which is respectively connected to the connecting rod and the second handle to connect the second blade body to the cover body. After the force is removed, the moved second blade is reset and the body is reset to close the second opening.

[0031] In another specific embodiment of the device for preparing umbilical cord segments, the first container and the second container are both cylindrical and coaxial.

[0032] Another specific embodiment of the device for making umbilical cord segments also includes a pin, which is inserted into a first connecting member set at the outer edge of the side wall of the first container and a second connecting member set at the outer edge of the side wall of the second container, and is assembled with the first connecting part and the second connecting part, with clearance fit, such as: the first connecting part and the second connecting part rotate around the pin (such as: pivot rotation).

[0033] In another specific embodiment of the device for making an umbilical cord segment, two ends of the first connecting portion are respectively assembled with the pin and the first supporting member.

[0034] In another specific implementation of the device for making an umbilical cord segment, two ends of the second connecting portion are respectively assembled with the pin and the first supporting member.

[0035] The ex vivo umbilical cord is placed in the device for preparing umbilical cord segments provided by the present invention, and umbilical cord segments of uniform length can be obtained, with a smooth cut surface and easy identification of the location of Wharton's jelly. The Wharton's jelly is then peeled off from the umbilical cord segments to obtain the Wharton's jelly, and umbilical cord mesenchymal stem cells are further prepared.

[0036] The device provided by the present invention significantly improves the efficiency of preparing Wharton's Jelly, and is also conducive to establishing standardized operating specifications for preparing umbilical cord mesenchymal stem cells, thereby achieving standardization of the preparation of Wharton's Jelly and umbilical cord mesenchymal stem cells.

[0037] The device provided by the present invention has a first container that allows the long umbilical cord to be easily placed between the blades, and cooperates with the second container to achieve uniform length of the umbilical cord segment. Placing the umbilical cord in the container allows the position of the umbilical cord to be relatively fixed during the cutting operation, reduces the irregularity of the incision caused by the umbilical cord slipping during the hand-held operation, and the cutting operation is more labor-saving.

[0038] With the cooperation of the cover body, the cut umbilical cord segment can be quickly discharged, the umbilical cord sample can be easily collected, and the length is uniform and the cut surface is smooth. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 A schematic structural diagram of an embodiment of a device for preparing an umbilical cord segment according to the present invention;

[0040] Figure 2 for Figure 1 The schematic structural diagram of the device for preparing umbilical cord segments of the present invention from another angle is shown;

[0041] Figure 3 for Figure 1 A schematic structural diagram of another state of the device for preparing an umbilical cord segment according to the present invention is shown;

[0042] Figure 4 for Figure 3 The schematic structural diagram of the device for preparing umbilical cord segments of the present invention from another angle is shown;

[0043] Figure 5 for Figure 1 The schematic structural diagram of a container of an embodiment of the device for preparing an umbilical cord segment of the present invention is shown;

[0044] Figure 6 for Figure 1 The structural schematic diagram of an embodiment of the cover body of the device for preparing the umbilical cord segment of the present invention is shown;

[0045] Figure 7 for Figure 1 The schematic structural diagram of a tool of an embodiment of the device for producing an umbilical cord segment of the present invention is shown;

[0046] Figure 8 for Figure 1 The structural schematic diagram of an embodiment of a supporting member of the device for preparing an umbilical cord segment of the present invention is shown;

[0047] Fig. 9 for Figure 1 The figure is a schematic structural diagram of an embodiment of the elastic mechanism of the device for preparing the umbilical cord segment of the present invention. DETAILED DESCRIPTION

[0048] The technical solution of the present invention is described in detail below with reference to the accompanying drawings. The embodiments of the present invention are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

[0049] Figure 1 This is a schematic structural diagram of an embodiment of a device for preparing an umbilical cord segment according to the present invention. Figure 2 for Figure 1 The schematic diagram of the structure of the device for preparing the umbilical cord segment of the present invention from another angle is shown. Figure 3 for Figure 1The schematic diagram of the structure of the device for preparing the umbilical cord segment of the present invention in another state is shown. Figure 1 , Figure 2 and Figure 3 As shown, the device for preparing the umbilical cord segment of this embodiment includes a first container 110 , a second container 120 , a first knife body 410 , a second knife body 420 and a cover body 300 .

[0050] Figure 5 for Figure 1 The schematic diagram of the structure of the container of the device for preparing the umbilical cord segment of the present invention is shown in FIG. Figure 1 , Figure 2 and Figure 3 ,like Figure 5 As shown, in this embodiment, the first container 110 has openings at both ends, which is used to accommodate the umbilical cord, and a first connector 111 is arranged at its outer edge. A second container 120 is arranged below it, which includes a first opening 121 and a second opening 122, and a second connector 123 arranged at the outer edge of the side wall. The first opening 121 is connected to the opening of the first container 110, and the end surface of the first opening 121 does not contact the lower end surface of the first container 120, and a shear space 130 is arranged therebetween. The cover body 300 controls the second opening 122 to be in a closed or open state.

[0051] When the first container 110 and the second container 120 are both cylindrical and coaxial, the first container 110 and the second container 120 form a cylindrical or truncated cone shape, and the end surface of the first opening 121 and the lower end surface of the first container 120 are circular. It should be understood that when the lower end outer wall of the first container 110 and the upper end outer wall of the second container 120 are partially connected, the shape of the end surface of the first opening 121 and the lower end surface of the first container 120 will be changed, so that the end surface of the first opening 121 and the lower end surface of the first container 120 present a non-circular shape, such as: a semicircular surface, a fan-shaped surface, a triangular surface or a quadrilateral surface. The pin 200 is inserted into the first connecting member 111 and the second connecting member 123 to connect the first container 110 and the second container 120, and fix the shape and volume of the shear space 130.

[0052] Figure 7 for Figure 1 The schematic diagram of the structure of the tool of the device for making umbilical cord segments of the present invention is shown in FIG. Figure 1 , Figure 2 and Figure 3 ,like Figure 7As shown, in this embodiment, the first blade body 410 includes a first blade portion 411, a first connecting portion 412 and a first handle portion 413. The first blade portion 411 is disposed in the shearing space 130, and the first handle portion 413 is disposed outside the side wall of the first container 120. One end of the first connecting portion 412 is assembled with the pin 400 and is clearance-matched, and rotates around the pin 200 to drive the first blade portion 411 to move in the shearing space 130 (e.g., pivot rotation).

[0053] The second blade body 420 includes a second blade portion 421, a second connection portion 422, and a second handle portion 423. The second blade portion 421 is disposed in the shearing space 130, and the second handle portion 423 is disposed outside the side wall of the second container. One end of the second connection portion 422 is assembled with the pin 200 and is clearance-matched, and rotates around the pin 400 to drive the second blade portion 421 to move in the shearing space 130 (e.g., pivot rotation).

[0054] When no force is applied, the first blade 411 and the second blade 421 are in the shearing space 130 , the umbilical cord is separated from the body and enters between the two blades, and then reaches the bottom surface of the other end of the second container 120 , that is, the second opening 122 closed by the cover 300 .

[0055] Figure 6 for Figure 1 The structure diagram of the cover of the device for making umbilical cord segments of the present invention is shown in FIG. Figure 1 , Figure 2 , Figure 3 and Figure 4 ,like Figure 6 As shown, the cover 300 includes a body 310 and a connecting rod 320. The body 310 covers the second opening 122 to close and open the second opening 122. One end of the connecting rod 320 is disposed on the body 310. A force is applied to the other end thereof to displace the body 310 to open the second opening 122. When the force is removed, the body 310 is reset to close the second opening 122. In order to facilitate the closing of the second opening 122 by the cover, in this embodiment, the connecting rod 320 is assembled on the lower edge of the second container 120 so that the connecting rod 320 is pivoted.

[0056] In this embodiment, the first supporting member 510 is connected to the connecting rod 320 and the first connecting portion 412 respectively, so that the tool is connected to the cover body, and after the force is removed, the first blade 411 that has moved is reset, and the body 310 is reset, closing the second opening 122. The second supporting member 520 is also connected to the connecting rod 320 and the second connecting portion 422 respectively, so that the tool is connected to the cover body, and after the force is removed, the second blade 421 that has moved is reset, and the body 310 is reset, closing the second opening 122.

[0057] Figure 8 for Figure 1 The structural schematic diagram of an embodiment of the supporting member of the device for making umbilical cord segments of the present invention is shown in FIG. Figure 8 As shown, the support member 500 includes a sliding rod 530 , and springs 540 are arranged at both ends of the sliding rod 530 , so that the support member 500 can adapt to the closing and opening operations of the second opening 122 of the body 310 after being connected to the connecting parts 412 , 422 of the blade body and the connecting rod 320 .

[0058] Fig. 9 for Figure 1 The structural diagram of an embodiment of the elastic mechanism of the device for making umbilical cord segments of the present invention is shown in FIG. Figure 1 ,like Fig. 9 As shown, in this embodiment, an elastic mechanism 600 is further provided, with its two ends respectively abutting against the outer wall of the second container 120 and the connecting rod 320 , and undergoing elastic deformation under the action of external force, and recovering after the force is removed, thereby resetting the body 310 and closing the second opening 122 .

[0059] The ex vivo umbilical cord is placed into the device for preparing umbilical cord segments provided in this embodiment from one end opening of the first container, passes through the space between the blades, and stops at the bottom surface of the other end of the second container (ie, the closed second opening 122).

[0060] Then, under the action of external force, the blade of the cutter moves from the container cavity toward the notch formed by the shearing space 130 and the container side wall, and gradually approaches the outer wall of the container cavity to cut the umbilical cord in the container cavity. At the same time, under the action of the cutter body, the cover body connected to the cutter body covering the second opening 122 opens, exposing the opening at the other end of the container, so that the cut umbilical cord segment is discharged from the second opening 122.

[0061] When the external force is removed, the blade returns to the container cavity; at the same time, the cover body connected to the blade body closes the opening at the other end of the container and covers the bottom surface of the other end of the container.

[0062] By repeating this process, umbilical cord segments of uniform length can be obtained, with a smooth cut surface, and the location of Wharton's jelly in each umbilical cord segment can be easily identified. Then the Wharton's jelly is peeled off from the umbilical cord segment, for example: the umbilical cord is cut along the umbilical vein, and after unfolding, the umbilical vein and two umbilical arteries are scraped off with a scalpel, and then the outermost membrane of the umbilical cord is removed with a scalpel. The remaining white jelly-like substance is the Wharton's jelly, and the umbilical cord mesenchymal stem cells are further prepared.

[0063] The use of the device provided in this embodiment in combination with the corresponding method can significantly improve the efficiency of preparing Wharton's Jelly, and is also conducive to establishing standardized operating specifications for the preparation of umbilical cord mesenchymal stem cells, thereby achieving standardization of the preparation of Wharton's Jelly and umbilical cord mesenchymal stem cells.

Claims

1. A method for preparing an umbilical cord segment, characterized in that include: First, the ex vivo umbilical cord is inserted from the opening at one end of the container, passes through the blade of the knife body, and stops at the bottom surface of the other end of the container; Then, under the action of external force, the blade of the knife body moves from the container cavity toward the notch on the container side wall, and gradually approaches the outer wall of the container cavity to cut the umbilical cord in the container cavity; at the same time, Under the force of the knife body, the cover body covering the bottom surface of the other end of the container and connected to the knife body is opened to expose the opening of the other end of the container, so that the cut umbilical cord segment is discharged from the opening of the other end of the container; When the external force is removed, the blade of the knife body returns to the container cavity; at the same time, The cover body connected to the knife body closes the opening at the other end of the container and covers the bottom surface of the other end of the container; The container comprises: A first container, with both ends opened, for accommodating the umbilical cord; A second container is placed below the first container and comprises a first opening and a second opening, wherein the first opening is in communication with the opening of the first container, an upper end surface of the first opening is not in contact with a lower end surface of the first container, and a shear space is provided therebetween; The cover body comprises a body and a connecting rod. The body covers the second opening to close and open the second opening. One end of the connecting rod is arranged on the body. A force is applied to the other end to displace the body to open the second opening. When the force is removed, the body is reset to close the second opening. The knife body comprises: A first blade body, comprising a first blade portion, a first connecting portion and a first handle portion, wherein the first blade portion is disposed in the shearing space, the first handle portion is disposed outside the side wall of the first container or outside the side wall of the second container, and the first connecting portion is used to drive the first blade portion to move in the shearing space; The second blade body includes a second blade portion, a second connecting portion and a second handle portion. The second blade portion is arranged in the shearing space, the second handle portion is arranged outside the side wall of the first container or outside the side wall of the second container, and the second connecting portion is used to drive the second blade portion to move in the shearing space.

2. The method according to claim 1, characterized in that include: A first supporting member is connected to the connecting rod and the first handle, respectively, so as to realize the connection between the first blade body and the cover body, and after the force is removed, the first blade portion that has moved is reset, and the body is reset, so as to close the second opening; The second supporting member is respectively connected to the connecting rod and the second handle to realize the connection between the second blade body and the cover body. After the force is removed, the moved second blade is reset and the body is reset to close the second opening.

3. The method according to claim 1, characterized in that It also includes an elastic mechanism, the two ends of which are respectively against the outer wall of the second container and the connecting rod. It is elastically deformed by external force, and recovers after the force is removed, so that the body is reset and the second opening is closed.

4. The method according to claim 1, characterized in that The first container and the second container are both cylindrical and coaxial.

5. The method according to claim 1, characterized in that It also includes a pin, which is inserted into a first connecting piece arranged at the outer edge of the side wall of the first container and a second connecting piece arranged at the outer edge of the side wall of the second container.

6. The method according to claim 5, characterized in that The pin is assembled with the first connecting part with clearance fit, and the first connecting part rotates around the pin.

7. The method according to claim 5, characterized in that The pin is assembled with the second connecting part with clearance fit, and the second connecting part rotates around the pin.

8. The method according to any one of claims 1 to 7, which is applied to the separation and preparation of Wharton gum.

9. The method according to any one of claims 1 to 7, which is used in preparing umbilical cord mesenchymal stem cells.

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