Use of a pharmaceutical composition in preparing an antibacterial agent

Abstract

The invention provides a use of a Chinese medicine compound prepared from 70 to 100 parts of rhubarb, 70 to 100 parts of sodium sulfate and 70 to 100 parts of gallnut in preparing an antibacterial agent. The Chinese medicine compound of the invention has a simple medicinal taste, has good antibacterial properties for bacteria (such as Staphylococcus aureus and Escherichia coli) and fungi (such as Candida albicans), has low cost and good effect, can meet the product standard of the elimination product, has pure Chinese medicine raw materials, has little toxic and side effects, shows good stability and effectiveness in accelerated storage experiments, has a long shelf life, and has good application prospects.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and specifically relates to use of a pharmaceutical composition in preparing an antibacterial agent. Background Art

[0002] Bacteria and fungi are the two most common types of pathogens. After infecting organisms, they can cause many diseases such as pneumonia and gastroenteritis. Therefore, inhibiting and killing the growth of bacteria and fungi is the key to preventing and treating related diseases.

[0003] Since the advent of penicillin, antibiotics have become the most widely used antibacterial agents. They have been applied in clinical practice, achieving good disease treatment effects and saving many lives. However, the abuse of antibiotics has led to the emergence of bacterial resistance, and long-term use of antibiotics can easily lead to adverse reactions in the body. Therefore, further exploration of antibacterial agents with good antibacterial effects, low resistance and few side effects has become an urgent need.

[0004] Compared with Western medicines such as antibiotics, Chinese medicine has a unique pharmacodynamic mechanism, less toxic side effects, and is not prone to drug resistance. Therefore, with the development of evidence-based medicine, the study of the antibacterial and anti-inflammatory effects of Chinese medicine has become a hot topic. Chinese scholars began to study the antibacterial effects and antibacterial components of Chinese medicine as early as the 1950s. With the continuous deepening of research, the antibacterial activity of many Chinese medicinal materials has been discovered. For example, Zhang Xiaoli et al. compared the in vitro antibacterial effects of gallic acid and gallic acid and found that they had inhibitory effects on Grapevine, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis, and Escherichia coli (Zhang Xiaoli, Li Xue, Gao Suni, Li Kangshe. Comparative study on the in vitro antibacterial effects of gallic acid and gallic acid [J]. China Pharmaceutical Industry, 2018, 27(23): 29-31.). Sheng Jiejing et al. compared the antibacterial activity of rhubarb and its processed products and confirmed that rhubarb and its processed products had different degrees of bactericidal effects on Escherichia coli, Staphylococcus aureus, Staphylococcus albicans, Bacillus subtilis, and Candida albicans.

[0005] At the same time, there are also many reports on the antibacterial effects of Chinese medicine composites. For example, Chinese patent CN113633685B discloses a composite composed of seven herbs: scutellaria, honeysuckle, isatis root, forsythia, hawthorn, mint, and gallnut, and verifies its antibacterial effects on Escherichia coli and Staphylococcus aureus. Another example is patent application CN114432387A, which also discloses an antibacterial composite and verifies its antibacterial effects on Escherichia coli, Staphylococcus aureus, and beta-hemolytic streptococci, and has potential value in treating gynecological inflammation.

[0006] However, the antibacterial compounds reported so far have many different flavors, are relatively expensive, and their antibacterial effects still need to be further improved. Summary of the invention

[0007] The object of the present invention is to provide a composition with a simplified formula and excellent antibacterial effect for use in an antibacterial agent.

[0008] The present invention provides a use of a pharmaceutical composition in the preparation of an antibacterial agent, wherein the pharmaceutical composition comprises the following raw materials in parts by weight:

[0009] 70-100 parts of rhubarb, 70-100 parts of Glauber's salt, and 70-100 parts of gallnut.

[0010] Furthermore, the pharmaceutical composition is prepared from the following raw materials in parts by weight:

[0011] 80-90 parts of rhubarb, 80-90 parts of sodium sulfate, and 80-90 parts of gallnut.

[0012] Furthermore, the pharmaceutical composition is prepared from the following raw materials in parts by weight:

[0013] 90 parts of rhubarb, 90 parts of sodium sulfate, and 90 parts of gallnut.

[0014] Furthermore, the antibacterial agent is a preparation for killing bacteria and / or fungi, or a preparation for inhibiting the growth of bacteria and / or fungi; and / or, the preparation is an external preparation.

[0015] Furthermore, the external preparation is a lotion.

[0016] Furthermore, the bacteria are Gram-positive bacteria or Gram-negative bacteria;

[0017] The Gram-positive bacteria include: Staphylococcus, Streptococcus, Pneumococcus, Bacillus anthracis, Bacillus diphtheriae, Bacillus tetanus; the Staphylococcus includes Staphylococcus aureus;

[0018] The Gram-negative bacteria include: Escherichia coli, Proteus, Klebsiella pneumoniae, Brucella, Pseudomonas aeruginosa, Haemophilus influenzae, Haemophilus parainfluenzae, Acinetobacter, Bordetella parapertussis, Bordetella cholerae, and Bacillus parahaemolyticus;

[0019] The fungi include: Candida, Geotrichum, Trichosporon, Malassezia; the Candida include Candida albicans;

[0020] And / or, the antibacterial agent is a preparation for resistant bacteria; the resistant bacteria include methicillin-resistant Staphylococcus aureus, multidrug-resistant Mycobacterium tuberculosis, extended-spectrum β-lactamase bacteria, vancomycin-resistant Enterococcus, carbapenem-resistant Enterobacteriaceae, multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Acinetobacter baumannii.

[0021] Furthermore, the antimicrobial agent is a drug for preventing and treating diseases caused by bacterial and / or fungal infections.

[0022] Furthermore, the diseases caused by the bacterial and / or fungal infection are gynecological diseases, men's diseases, gastroenteritis, pneumonia, fasciitis, mastitis or cow mastitis.

[0023] Furthermore, the gynecological disease is Bartholin's gland cyst, pelvic inflammatory disease and / or vaginitis; the andrological disease is the prevention and treatment of prostatitis.

[0024] Furthermore, the medicine is a medicine for preventing postoperative infection and / or promoting wound healing.

[0025] The "glauber's salt" described in the present invention is a sulfate mineral Glauber's salt of the Glauber's salt family, which is a crystal mainly containing sodium sulfate decahydrate after processing and refinement, and is a traditional Chinese medicine.

[0026] The beneficial effects of the invention are as follows: the invention provides a use of a Chinese medicine compound made of rhubarb, sodium sulfate and gallnut in an antibacterial agent. The compound has simple flavors, has good antibacterial properties against bacteria (such as Staphylococcus aureus and Escherichia coli) and fungi (such as Candida albicans), has low cost and good effects, and can meet the standards for products with the "Xiaozihao" mark. The invention adopts pure Chinese medicine raw materials, has small toxic and side effects, and shows good stability and effectiveness in accelerated storage experiments. The storage time can be as long as 2 years, and has good application prospects.

[0027] Obviously, according to the above contents of the present invention, in accordance with common technical knowledge and customary means in the art, without departing from the above basic technical ideas of the present invention, other various forms of modification, replacement or change may be made.

[0028] The above contents of the present invention are further described in detail below through specific implementation methods in the form of embodiments. However, this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples. All technologies realized based on the above contents of the present invention belong to the scope of the present invention. DETAILED DESCRIPTION

[0029] The raw materials and equipment used in the present invention are all known products, which are obtained by purchasing commercial products. The preparation process of the lotion made from the composition of the present invention is mature and can be made by referring to the conventional method in the prior art.

[0030] Example 1. Preparation of the compound antibacterial lotion of the present invention

[0031] Recipe: 90g rhubarb, 90g Glauber's salt, and 90g gallnut.

[0032] Preparation method: Weigh the above medicinal ingredients according to the ratio, add 1800 mL of water and soak for 40 minutes, boil and keep boiling for 30 minutes, filter, concentrate the filtrate to 100 mL equivalent to 30 g of the original medicine, and package it to obtain a lotion.

[0033] Example 2: Preparation of the compound antibacterial lotion of the present invention

[0034] Recipe: 70g rhubarb, 100g Glauber's salt, and 100g gallnut.

[0035] Preparation method: Same as Example 1

[0036] Example 3: Preparation of the compound antibacterial lotion of the present invention

[0037] Recipe: 100g rhubarb, 70g Glauber's salt, and 70g gallnut.

[0038] Preparation method: Same as Example 2

[0039] Example 4: Preparation of the compound antibacterial lotion of the present invention

[0040] Recipe: 70g rhubarb, 70g Glauber's salt, and 100g gallnut.

[0041] Preparation method: Same as Example 1

[0042] Example 5. Preparation of the compound antibacterial lotion of the present invention

[0043] Recipe: 80g rhubarb, 90g Glauber's salt, and 90g gallnut.

[0044] Preparation method: Same as Example 1.

[0045] Example 6. Preparation of the compound antibacterial lotion of the present invention

[0046] Recipe: 90g rhubarb, 80g Glauber's salt, and 90g gallnut.

[0047] Preparation method: Same as Example 1.

[0048] Example 7. Preparation of the compound antibacterial lotion of the present invention

[0049] Recipe: 70g rhubarb, 100g Glauber's salt, 70g gallnut.

[0050] Preparation method: Same as Example 1.

[0051] Example 8. Preparation of the compound antibacterial lotion of the present invention

[0052] Recipe: 100g rhubarb, 70g Glauber's salt, and 100g gallnut.

[0053] Preparation method: Same as Example 1.

[0054] The beneficial effects of the present invention are demonstrated by experimental examples below.

[0055] Experimental Example 1: Experiment on the disassembly of the compound antibacterial lotion of the present invention

[0056] 1. Equipment

[0057] 1. Test samples

[0058] 1# Rhubarb water extract: Add 200mL of water to 30g of rhubarb, soak for 40 minutes, boil for 30 minutes, filter, and concentrate the filtrate to 100mL equivalent to 30g of the original drug.

[0059] 2# Gallnut water extract: Add 200mL of water to 30g of Gallnut, soak for 40 minutes, boil for 30 minutes, filter, and concentrate the filtrate to 100mL equivalent to 30g of the original medicine.

[0060] 3# 30% Sodium Sulfate Solution: Dissolve 30g Sodium Sulfate in 100mL hot water.

[0061] 4# Rhubarb and Chinese gallnut water extract: Add 400mL of water to 30g of rhubarb and Chinese gallnut, soak for 40 minutes, boil for 30 minutes, filter, and concentrate the filtrate to 100mL which is equivalent to 30g of the original medicine.

[0062] 5# Rhubarb and Sodium Sulfate Water Extract: Add 400mL of water to 30g of rhubarb and Sodium Sulfate, soak for 40 minutes, boil for 30 minutes, filter, and concentrate the filtrate to 100mL which is equivalent to 30g of the original drug.

[0063] 6# Gallic acid and Sodium Sulfate Water Extract: Add 30g each of Gallic acid and Sodium Sulfate to 400mL of water, soak for 40 minutes, boil for 30 minutes, filter, and concentrate the filtrate to 100mL which is equivalent to 30g of the original medicine.

[0064] 7# Manghuang lotion 1 (prepared according to Example 1)

[0065] 8# Manghuang Lotion 2 (the medicinal material ratio is 70g of rhubarb, 100g of sodium sulfate, and 70g of gallnut, prepared according to the method of Example 1)

[0066] 9# Manghuang Lotion 3 (the medicinal material ratio is 100g of rhubarb, 70g of sodium sulfate, and 100g of gallnut, prepared according to the method of Example 1)

[0067] 10# Manghuang Lotion 4 (the medicinal material ratio is 100g of rhubarb, 70g of sodium sulfate, and 70g of gallnut, prepared according to the method of Example 1)

[0068] 2. Test strains: Escherichia coli (8099), take its 5th and 6th generation fresh slant culture for testing; Staphylococcus aureus (ATCC 6538), take its 5th and 6th generation fresh slant culture for testing; Candida albicans (ATCC 10231) take its 6th generation fresh slant culture for testing. The above strains are provided by Guangdong Food Microbiological Safety Engineering Technology Research and Development Center.

[0069] 3. Instruments and equipment: KC-SP-YQ-179 electric constant temperature incubator (DHP-9272), KC-SP-YQ-586 biological safety cabinet (HR50-IIA2).

[0070] 2. Experimental Methods

[0071] 1. Inspection basis: Appendix C4 of GB 15979-2002 Hygiene Standard for Disposable Sanitary Products.

[0072] 2. Test conditions: temperature 20-23°C, relative humidity 50-54%.

[0073] 3. Sample preparation: original sample

[0074] 3. Experimental Results

[0075] Table 1 Antibacterial effects of different compound antibacterial lotions

[0076]

[0077]

[0078] From the above results, it can be seen that for Escherichia coli, the rhubarb water extract containing 30g of the original medicine per 100mL and the 30% sodium sulfate solution have no antibacterial effect, and the gallnut water extract contains 30g of the original medicine per 100mL, and the antibacterial rate exceeds 50% in the action time of 2min, that is, it has an antibacterial effect, and the action time exceeds 5min, and the antibacterial rate exceeds 90%, that is, it produces a strong antibacterial effect. However, when rhubarb, sodium sulfate and gallnut are compounded in pairs to form the original medicine containing the same dose, the antibacterial effect is significantly reduced relative to the gallnut water extract, and even has no antibacterial effect. When rhubarb, sodium sulfate and gallnut are combined to form the original medicine containing the same dose, the antibacterial effect is significantly enhanced, and the antibacterial rate exceeds 90% in the action time of 2min compared with the gallnut water extract, reaching a strong antibacterial effect, especially the antibacterial lotion of Example 1 made by the three in a ratio of 1:1:1, the antibacterial rate can reach 100% in the action of 2min.

[0079] For Staphylococcus aureus and Candida albicans, the antibacterial rates of rhubarb water extract, mirabilite solution and gallnut water extract were lower than 50%, and the antibacterial effect was poor. When rhubarb, mirabilite and gallnut were compounded in pairs to form a solution containing the same dose of native medicine, the antibacterial effect was not improved. However, when rhubarb, mirabilite and gallnut were compounded to form a solution containing 30g of native medicine per 100mL, the antibacterial effect was greatly improved, and the antibacterial rate exceeded 50%. In particular, the antibacterial lotion of Example 1 obtained by compounding in a ratio of 1:1:1 reached 100% for Staphylococcus aureus after 10 minutes of action, and reached 100% for Candida albicans after 2 minutes of action, producing a strong antibacterial effect.

[0080] From the perspective of the antibacterial effect on bacteria and fungi, the compound antibacterial lotion of the present invention plays a synergistic role by compounding rhubarb, sodium sulfate and gallnut in a specific ratio.

[0081] Experimental Example 2: Physical property test of the compound antibacterial lotion of the present invention

[0082] 1. pH value

[0083] 1. Equipment

[0084] 1.1 Test sample: the lotion stock solution of Example 1.

[0085] 1.2. Instruments: KC-SPYQ-054 pH meter (PHS-3C).

[0086] 1.3. Calibration buffer solution: Potassium hydrogen phthalate buffer solution (pH = 4.00, 25 ℃ ); mixed phosphate solution (pH = 6.86, 25 ℃ ); Borax buffer solution (pH = 9.18, 25 ℃ ).

[0087] 2. Methods

[0088] 2.1. Inspection basis: Technical Specifications for Disinfection (2002 edition) 2.2.1.4.

[0089] 2.2. Test conditions: Temperature 20~22 ℃ , relative humidity 46-61%. The test was repeated twice.

[0090] 2.3. Sample treatment: as is.

[0091] 3. Results

[0092] The pH values ​​of the two batches of detergent stock solutions were measured twice, and the pH values ​​of the detergents were 4.00 and 3.76 respectively. The results are shown in Table 2.

[0093] Table 2 pH value measurement results

[0094]

[0095] Combined with the test results in Table 2, the pH value range of the compound antibacterial lotion was determined to be 3.8-4.2 through multiple tests.

[0096] 2. Osmotic pressure

[0097] 1.1 Test sample: the lotion stock solution of Example 1.

[0098] 1.2. Instruments: Osmotic pressure molar concentration meter SMC 30.

[0099] 2. Methods

[0100] 2.1.Test basis: General Rules 0632 of Part IV of the Chinese Pharmacopoeia 2020 Edition, Osmotic Pressure Molar Concentration Determination Method.

[0101] 2.2. Test conditions: Temperature 20~22 ℃ , relative humidity 46~61%.

[0102] 2.3. Sample treatment: as is.

[0103] 3. Results

[0104] The osmotic pressure of the compound antibacterial lotion was determined to be 550-580 mmol / liter through multiple measurements of multiple batches of lotion stock solutions.

[0105] Experimental Example 3: Safety Performance Test of the Compound Antibacterial Lotion of the Present Invention

[0106] 1. Materials and Animals

[0107] 1.1 Test sample: the lotion stock solution of Example 1.

[0108] 1.2 Experimental animals

[0109] Species / strain: rabbit / New Zealand rabbit; Grade: ordinary grade; Number and gender: 12, including 6 males and 6 females; Weight: 2.0-2.5 kg;

[0110] Breeding unit①: Jinan Xilingjiao Breeding and Breeding Center; Laboratory Animal Production License No.: SCXK(Lu)20200004; Laboratory Animal Quality Certificate No.: No.370822211100065962, No370822211100062245;

[0111] Breeding unit②: Shandong Ailek Biotechnology Co., Ltd.; Laboratory Animal Production License No.: SCXK(Lu)2019 0006; Laboratory Animal Quality Certificate No.: No.370839221100042373.

[0112] 2. Skin irritation test

[0113] 1. Methods

[0114] 1.1. Inspection basis: "Technical Specifications for Disinfection" (2002 Edition) 2.3.3 Skin irritation test

[0115] 1.2. Sample preparation: Use the original sample without preparation.

[0116] 1.3. Test methods

[0117] 1.3.1 Animal preparation: 24 hours before the experiment, remove the hair on both sides of the spine of the New Zealand rabbit with a depilatory agent without damaging the skin. The hair removal area is about 3cmx3cm on the left and right sides.

[0118] 1.3.2 Exposure method: Apply 0.5g of the test substance to the left skin the next day, and use the right skin as a blank control. Wash with water 4 hours after application to remove the residue. Apply once a day for 14 consecutive days.

[0119] 1.3.3 Symptom observation: Observe the results 24 hours after each application. Score the stimulation reaction of animal skin erythema and edema according to Table 2-11 in 2.3.3 of "Technical Specifications for Disinfection" (2002 Edition). In order to facilitate the application of the test substance and the observation of the results, the hair should be cut if necessary. The treatment method of the control area is the same as that of the test area.

[0120] 1.3.4 Evaluation regulations: Calculate the average score (irritation index) of each animal per day according to the following formula, and evaluate the level of irritation intensity of the test substance on animal skin according to Table 2-12 in 2.3.3 of the Technical Specifications for Disinfection (2002 Edition).

[0121]

[0122] 2. Results

[0123] No skin erythema and edema were observed in the control area and the test area of ​​the New Zealand rabbit within 14 days after the poisoning. The average value of the skin irritation reaction score was 0. This indicates that the irritation intensity of the compound antibacterial lotion of the present invention on the New Zealand rabbit's multiple complete skin irritation tests is non-irritating, which meets the requirements of the "Disinfection Technical Specifications" (2002 edition).

[0124] 3. Acute eye irritation test

[0125] 1. Methods

[0126] 1.1. Inspection basis: Technical Specifications for Disinfection (2002 edition) 2.3.4 Acute eye irritation test.

[0127] 1.2. Sample preparation: Use the original sample without preparation.

[0128] 1.3 Test method:

[0129] 1.3.1 Animal preparation: Before the experiment, check both eyes of each New Zealand rabbit. If no abnormality is found, the rabbit can be used for the experiment.

[0130] 1.3.2 Exposure method: Take 0.1g of the test substance and drop it into the conjunctival sac of the left eye of the animal. The eye is passively closed for 4 seconds and then rinsed with saline after 30 seconds. The right eye is treated with saline as a normal control.

[0131] 1.3.3 Symptom observation: Observe the damage and recovery of the conjunctiva, iris and cornea of ​​New Zealand rabbits with naked eyes at 1h, 24h, 48h and 72h after eye drops. If no irritation reaction occurs within 72h, the test is terminated.

[0132] 1.3.4 Evaluation regulations: The acute irritation reaction of the cornea, iris and conjunctiva of New Zealand rabbits shall be scored according to Table 2-13 in 2.3.4 of Technical Specifications for Disinfection (2002 Edition), and the "average score" of corneal damage, iris damage, conjunctival congestion and conjunctival edema of each animal at three different observation times (24h, 48h and 72h) shall be calculated respectively (i.e. the sum of the scores of each animal at 24h, 48h and 72h divided by the number of observations 3). The irritation intensity of the test substance to the eyes of New Zealand rabbits shall be determined according to the average scores and recovery time of corneal, iris and conjunctival congestion and edema of the animals respectively according to the eye irritation reaction grading standards in Table 2-14 and Table 2-15 in 2.3.4 of Technical Specifications for Disinfection (2002 Edition).

[0133] 2. Results

[0134] No corneal damage, iris damage, conjunctival congestion and conjunctival edema were observed 24h, 48h and 72h after eye drops. The average score of eye irritation reaction was 0. This indicated that the damage type of the compound antibacterial lotion of the present invention to New Zealand rabbit eyes was non-irritating. It met the requirements of the Technical Specification for Disinfection (2002 edition).

[0135] IV. Vaginal mucosal irritation test

[0136] 1. Methods

[0137] 1.1. Inspection basis: Technical Specifications for Disinfection (2002) 2.3.5 Vaginal mucosal irritation test.

[0138] 1.2. Preparation of test substance: Use stock solution and prepare without any reagent.

[0139] 1.3 Test methods:

[0140] 1.3.1 Animal preparation: Before the experiment, check the vaginal opening of the animals. If there is no secretion, congestion, edema or other damage, they can be used for the experiment. The animals were divided into a poisoning group and a control group, with 3 animals in each group.

[0141] 1.3.2 Operating procedures

[0142] (1) Connect a blunt-tipped hose of about 8 cm in length to a 2 mL syringe. Fill the syringe and catheter with the test solution and set aside. Prepare one set for each animal. Fix the animal on its back, expose the perineum and vaginal opening, moisten the catheter with the test solution and gently insert it into the vagina (4 cm to 5 cm), and slowly inject 2 mL of the test solution with a syringe. Pull out the catheter to complete the infection. Repeat the infection every 24 hours for 5 consecutive days. The animals in the control group are treated in the same way with saline.

[0143] (2) 24 hours after the last exposure, the animals were killed by gas embolism, and the intact vagina was removed by dissection. The vagina was cut longitudinally and observed with the naked eye to see if there was no congestion or edema. The vagina was then placed in a 10% neutral buffered formalin solution and fixed for more than 24 hours. Tissues from the two ends and the center of the vagina were selected for preparation and HE staining for histopathological examination.

[0144] 1.3.3 Evaluation regulations

[0145] (1) Histopathological examination results The irritation response of the vaginal mucosa was scored according to Table 3.

[0146] (2) The scores of the stimulation responses of the three sites of the three animals in the experimental group were added together and then divided by the total number of observations (number of animals * 3) to obtain the average score of the vaginal mucosal stimulation response of the experimental group. The scoring method for the control group was the same as above.

[0147] (3) After subtracting the mean score of the control group from the mean score of the experimental group to obtain the stimulation index, the stimulation intensity was graded according to Table 4.

[0148] Table 3 Vaginal mucosal irritation response scoring criteria

[0149]

[0150]

[0151] Stimulus response score = A+B+C+D

[0152] Table 4 Vaginal mucosal irritation intensity classification

[0153]

[0154] 2. Results

[0155] The compound antibacterial lotion of the present invention has a vaginal mucosal irritation index of 0.89 on New Zealand rabbits. The reaction intensity of multiple vaginal mucosal irritation tests on New Zealand rabbits is extremely mild irritation, which meets the requirements of "Disinfection Technical Specifications" (2002 edition).

[0156] Experimental Example 4: Effect experiment on the stability of the compound antibacterial lotion of the present invention

[0157] 1. Equipment

[0158] 1. Test sample: the lotion stock solution of Example 1.

[0159] 2. Test strain: Candida albicans (ATCC 10231) was tested on fresh slant cultures of the 4th and 6th generations. The above strains were provided by Guangdong Food Microbiological Safety Engineering Technology Research and Development Center.

[0160] 3. Instruments and equipment: KC-SP-YQ-179 electric constant temperature incubator (DHP-9272), KC-SP-YQ-S86 biological safety cabinet (HR50-IIA2), KC-SP-YQ-125 constant temperature and humidity chamber (LHS-100CL).

[0161] 2. Experimental Methods

[0162] 1. Inspection basis: Appendix C4 and C6 of GB 15979-2002 Hygiene Standard for Disposable Sanitary Products.

[0163] 2. Test conditions: temperature 19-23°C, relative humidity 50-54%.

[0164] 3. Sample preparation: original sample

[0165] 3. Experimental Results

[0166] 1. Inhibitory effect on Candida albicans

[0167] 1.1 The antibacterial rate data of the antibacterial wash solution of Example 1 of the present invention on Candida albicans (ATCC 10231) after being prepared for 2 minutes, 5 minutes, 10 minutes and 20 minutes are shown in Table 5.

[0168] Table 5 Antibacterial effect of antibacterial lotion stock solution of Example 1 on Candida albicans

[0169]

[0170] Note: Negative control showed no sterile growth.

[0171] 1.2 The antibacterial wash solution of Example 1 of the present invention was prepared and stored at 54°C for 14 days. The antibacterial rate data of Candida albicans (ATCC 10231) at 2 min, 5 min, 10 min and 20 min are shown in Table 6.

[0172] Table 6 Antibacterial effect of antibacterial lotion stock solution of Example 1 on Candida albicans

[0173]

[0174] Note: Negative control showed no sterile growth.

[0175] 1.3 The antibacterial wash solution of Example 1 of the present invention was prepared and stored at 37°C for 90 days. The antibacterial rate data of the solution against Candida albicans (ATCC 10231) at 2 min, 5 min, 10 min and 20 min are shown in Table 7.

[0176] Table 7 Antibacterial effect of antibacterial lotion stock solution of Example 1 on Candida albicans

[0177]

[0178]

[0179] Note: Negative control showed no sterile growth.

[0180] It can be seen that according to GB 15979-2002 "Hygiene Standard for Disposable Hygiene Products", under experimental conditions, the antibacterial wash of the present invention has an average antibacterial rate of >99.96% against Candida albicans after 2 minutes, 5 minutes, 10 minutes and 20 minutes of action; the wash stored at 54°C and 37°C for 14 days and 90 days respectively still has a strong antibacterial effect on Candida albicans. It has a stability of 2 years and meets the requirements of GB 15979-2002 "Hygiene Standard for Disposable Hygiene Products".

[0181] In summary, the Chinese medicine compound lotion composed of rhubarb, sodium sulfate and gallnut has good antibacterial properties against bacteria (such as Staphylococcus aureus and Escherichia coli) and fungi (such as Candida albicans), has low cost and good effect, and can meet the product standards of the elimination product; the present invention adopts pure Chinese medicine raw materials, has small toxic and side effects, and shows good stability and effectiveness in accelerated storage experiments. The storage time can be up to 2 years, and has good application prospects.

Claims

1. Use of a pharmaceutical composition in preparing a drug for killing Candida albicans, It is characterized in that The pharmaceutical composition is prepared from the following raw materials in parts by weight: 90 parts of rhubarb, 90 parts of mirabilite, and 90 parts of gallnut; the medicine is an external preparation.

2. The use according to claim 1, It is characterized in that The external preparation is a lotion.

3. The use according to claim 1 or 2, It is characterized in that The medicine is used for preventing and treating gynecological diseases caused by Candida albicans infection.

4. The use according to claim 3, It is characterized in that The gynecological disease is Bartholin's gland cyst, pelvic inflammatory disease and / or vaginitis.

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