Compound plant essential oil microemulsion and its preparation method and application

Abstract

The present invention discloses a composite plant essential oil microemulsion and a preparation method thereof. The composite plant essential oil microemulsion comprises, by weight: 10 parts - 14 parts of composite plant essential oil, 24 parts - 40.9 parts of surfactant, 8 parts - 18 parts of co-surfactant, and 44 parts - 52 parts of pure water; the composite plant essential oil comprises, by weight: 60 parts - 80 parts of cinnamaldehyde and 20 parts - 40 parts of oregano oil; the HLB value of the surfactant is 13 - 15.5. The composite plant essential oil microemulsion provided by the present invention has better stability under normal temperature, high temperature and low temperature and other accelerated conditions, can effectively reduce the volatilization of essential oil, extend the storage time of essential oil, and can improve the animal production performance, antioxidant capacity and promote the development of immune organs when applied to livestock and poultry drinking water.

Description

Technical Field

[0001] The present invention relates to the technical field of compound plant essential oil microemulsions, and particularly to a compound plant essential oil microemulsion, a preparation method thereof, and an application thereof. Background Art

[0002] Plant essential oils are a general term for oily liquids extracted from plants with certain volatility. They are widely distributed in plants and can be extracted from flowers, leaves, roots, barks or whole plants. They have strong volatility and hydrophobicity, are insoluble in water, but soluble in organic solvents and lipids. According to research, plant essential oils have broad-spectrum and high-efficiency antibacterial activities, have good antibacterial effects on pathogenic bacteria such as Staphylococcus aureus, Streptococcus, Escherichia coli, and Candida albicans, and at the same time have advantages such as improving production performance, enhancing immunity, improving intestinal health, and preventing and treating diseases in livestock and poultry production.

[0003] Due to the high volatility and unique aromatic smell of plant essential oils, and their sensitivity to environmental factors such as light, heat, and oxygen, poor stability, and short antibacterial time when used alone, it is particularly important to select the type of essential oil and study technical measures for the stability of plant essential oils.

[0004] A microemulsion is a thermodynamically stable, transparent or translucent, isotropic liquid dispersion system spontaneously formed by an aqueous phase, an oil phase, a surfactant, and a co-surfactant in a certain proportion, with a particle size of 1 - 100 nm, and has a good effect on enhancing the water solubility and stability of the embedded substance. The present invention will explore a microemulsion system of compound essential oils to improve the stability of essential oils. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a compound plant essential oil microemulsion, which can have better stability under normal temperature, high temperature, low temperature and other accelerated conditions, effectively reduce the volatilization of essential oils, and extend the storage time of essential oils.

[0006] The technical problem to be solved by the present invention is also to provide a preparation method of a compound plant essential oil microemulsion, which has a simple process and can stably prepare the above-mentioned compound plant essential oil microemulsion with good performance.

[0007] To solve the above technical problems, the present invention provides a compound plant essential oil microemulsion, which comprises, by weight: 10 parts - 14 parts of compound plant essential oil, 24 parts - 40.9 parts of surfactant, 8 parts - 18 parts of co-surfactant, and 44 parts - 52 parts of pure water;

[0008] The compound plant essential oil comprises, by weight: 60 parts - 80 parts of cinnamaldehyde, and 20 parts - 40 parts of origanum oil;

[0009] The HLB value of the surfactant is 13 - 15.5.

[0010] In one embodiment, the surfactant is Tween-80 and / or polyoxyethylene castor oil 360.

[0011] In one embodiment, the co-surfactant is absolute ethanol.

[0012] In one embodiment, the mass ratio of the surfactant to the co-surfactant is (1-4):1.

[0013] In one embodiment, the mass ratio of cinnamaldehyde to oregano oil is (6-7):(3-4).

[0014] To solve the above problems, the present invention provides a method for preparing a composite plant essential oil microemulsion, comprising the following steps:

[0015] Mix 10 parts - 14 parts of the composite plant essential oil, 24 parts - 40.9 parts of the surfactant, 8 parts - 18 parts of the co-surfactant, and 44 parts - 52 parts of pure water, and stir evenly to obtain the finished product;

[0016] The composite plant essential oil comprises, by weight: 60 parts - 80 parts of cinnamaldehyde and 20 parts - 40 parts of oregano oil;

[0017] The HLB value of the surfactant is 13 - 15.5.

[0018] Correspondingly, the present invention provides the application of the composite plant essential oil microemulsion in livestock and poultry drinking water for improving animal production performance, antioxidant capacity, and promoting the development of immune organs.

[0019] Implementing the present invention has the following beneficial effects:

[0020] The composite plant essential oil microemulsion provided by the present invention is a safe, non-toxic, and environmentally friendly antibiotic alternative product, which has better stability under normal temperature, high temperature, and low temperature acceleration conditions, effectively reduces the volatilization of essential oils, and extends the storage time of essential oils. Moreover, the present invention can be applied to livestock and poultry drinking water, which can improve animal production performance, antioxidant capacity, and promote the development of immune organs. Detailed Embodiments

[0021] To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below.

[0022] Unless otherwise stated or there are contradictions, the terms or phrases used herein have the following meanings:

[0023] In the present invention, "its combination", "any combination thereof", "any combination mode thereof", etc. include all suitable combination modes of any two or more of the listed items.

[0024] In the present invention, "preferred" only describes the embodiments or examples with better effects, and it should be understood that it does not constitute a limitation to the protection scope of the present invention.

[0025] In the present invention, among the technical features described in an open-ended manner, it includes the closed technical solutions composed of the listed features, and also includes the open technical solutions containing the listed features.

[0026] In the present invention, regarding the numerical range, if there is no special description, it includes the two endpoints of the numerical range.

[0027] The present invention provides a composite plant essential oil microemulsion, which includes, by weight: 10 parts - 14 parts of composite plant essential oil, 24 parts - 40.9 parts of surfactant, 8 parts - 18 parts of co-surfactant, and 44 parts - 52 parts of pure water;

[0028] The composite plant essential oil includes, by weight: 60 parts - 80 parts of cinnamaldehyde, and 20 parts - 40 parts of origanum oil;

[0029] The HLB value of the surfactant is 13 - 15.5.

[0030] The composite plant essential oil microemulsion provided by the present invention is a safe, non-toxic and green environmental protection alternative to antibiotics. It has better stability under normal temperature, high temperature and low temperature acceleration conditions, can effectively reduce the volatilization of essential oil and extend the storage time of essential oil. Moreover, the present invention can be applied to livestock and poultry drinking water, and can improve animal production performance, antioxidant capacity and promote the development of immune organs.

[0031] Specifically, cinnamaldehyde is an aldehyde organic compound, which is a yellow viscous liquid and exists in large amounts in plants such as cinnamon. Origanum oil, origanum is the whole herb of Origanum vulgare L. of the genus Origanum in the Lamiaceae family, also known as Zhilizao, Ye Jingjie and other local names, and has certain functions of clearing heat, resolving dampness, dispelling summer heat, relieving exterior syndrome, regulating qi, sterilizing and antibacterial. The present invention selects a certain proportion of cinnamaldehyde and origanum oil to prepare the essential oil microemulsion, which can effectively improve animal production performance, antioxidant capacity and promote the development of immune organs. In one embodiment, the mass ratio of the cinnamaldehyde to the origanum oil is (6 - 7):(3 - 4).

[0032] Furthermore, the present invention obtains a composite plant essential oil microemulsion, which is a completely different dispersion system from the composite plant essential oil emulsion in the prior art, although the two are only one word different. First, emulsion generally refers to a dispersion system with a droplet radius between 300nm and 100μm. From the perspective of the diameter range of the droplets, most of them belong to a coarse dispersion system. The droplet radius of the dispersed phase of the microemulsion is in the range of 10-100nm, and it is a transparent or translucent system. Secondly, from the performance analysis, the colloidal system of the emulsion type is dynamically unstable and will tend to demulsify with the passage of time or changes in the external environment. Microemulsion can maintain thermodynamic stability under certain environmental conditions, and its stability is better than that of emulsion. Finally, from the composition, emulsifiers account for the main proportion of the components of the emulsion, in addition to a small amount of co-emulsifiers and surfactants. Microemulsion is a dispersion system formed spontaneously by mixing water, oil, a large amount of surfactants and co-surfactants. In other words, emulsions and microemulsions are different in terms of composition, preparation method, dispersed phase morphology, performance of the dispersion system, etc. It is difficult to develop microemulsions based on emulsions. If you want to develop a microemulsion with better performance, you need to overturn the original emulsion dispersion system and re-study the material composition and ratio of the microemulsion.

[0033] Under the above ratio, a composite plant essential oil microemulsion with a certain stability can be obtained, but the composite plant essential oil microemulsion prepared under the above specific composition ratio may only maintain kinetic stability under specific environmental conditions, and may become unstable once the environmental conditions change, among which the influence of temperature is particularly obvious.

[0034] The inventors have found that the stability of the composite plant essential oil microemulsion at high and low temperatures can be improved by selecting a specific surfactant. In one embodiment, the HLB value of the surfactant is 13-15.5. In the present invention, the oil phase is a specific content of cinnamaldehyde and oregano oil, which, when mixed with a surfactant with an HLB value of 13-15.5 according to the above ratio, can obtain a microemulsion with high and low temperature stability. Preferably, the surfactant is Tween-80 and / or polyoxyethylene castor oil 360. More preferably, the surfactant is Tween-80 and polyoxyethylene castor oil 360, and there is a small gap in the HLB values ​​of the two, and a microemulsion with more stable physical and chemical properties can be obtained after compounding.

[0035] In addition, the formation of the composite plant essential oil microemulsion of the present invention cannot be separated from the participation of a cosurfactant. In one embodiment, the cosurfactant is anhydrous ethanol. The cosurfactant can adjust the HLB value of the system to a more ideal range, and can reduce the mutual repulsion and charge repulsion of the surfactants, so that the interface film has good flexibility and fluidity, the interface is easy to bend, and the microemulsion is easy to form.

[0036] Furthermore, the mass ratio of the surfactant to the co-surfactant will affect the stability of the microemulsion. If the addition amount of the surfactant is too low, the emulsifying ability will be too weak to form a stable microemulsion; if the addition amount of the surfactant is too high, the microemulsion will break. In one embodiment, the mass ratio of the surfactant to the co-surfactant is (1 - 4):1.

[0037] Correspondingly, the present invention provides a method for preparing a composite plant essential oil microemulsion, comprising the following steps:

[0038] Mix 10 parts - 14 parts of the composite plant essential oil, 24 parts - 40.9 parts of the surfactant, 8 parts - 18 parts of the co-surfactant, and 44 parts - 52 parts of pure water, and stir evenly to obtain the finished product;

[0039] The composite plant essential oil comprises, by weight: 60 parts - 80 parts of cinnamaldehyde and 20 parts - 40 parts of origanum oil;

[0040] The HLB value of the surfactant is 13 - 15.5.

[0041] Moreover, the present invention provides the application of the composite plant essential oil microemulsion in livestock and poultry drinking water for improving animal production performance, antioxidant capacity, and promoting the development of immune organs.

[0042] The following further illustrates the present invention with specific examples:

[0043] Example 1

[0044] This example provides a composite plant essential oil microemulsion, which comprises, by weight: 10 parts of the composite plant essential oil, 30 parts of Tween - 80, 15 parts of absolute ethanol, and 45 parts of pure water;

[0045] The composite plant essential oil comprises 60 parts of cinnamaldehyde and 40 parts of origanum oil.

[0046] Example 2

[0047] This example provides a composite plant essential oil microemulsion, which comprises, by weight: 12 parts of the composite plant essential oil, 28.5 parts of polyoxyethylene castor oil 360, 9.5 parts of absolute ethanol, and 50 parts of pure water;

[0048] The composite plant essential oil comprises 60 parts of cinnamaldehyde and 40 parts of origanum oil.

[0049] Example 3

[0050] This embodiment provides a composite plant essential oil microemulsion, which includes, by weight: 13 parts of composite plant essential oil, 24.8 parts of polyoxyethylene castor oil 360, 12.4 parts of Tween-80, 9.3 parts of absolute ethanol, and 40.5 parts of pure water;

[0051] The composite plant essential oil includes 70 parts of cinnamaldehyde and 30 parts of oregano oil.

[0052] Example 4

[0053] This embodiment provides a composite plant essential oil microemulsion, which includes, by weight: 14 parts of composite plant essential oil, 12.4 parts of polyoxyethylene castor oil 360, 24.8 parts of Tween-80, 9.3 parts of absolute ethanol, and 40.5 parts of pure water;

[0054] The composite plant essential oil includes 70 parts of cinnamaldehyde and 30 parts of oregano oil.

[0055] Comparative Example 1

[0056] This embodiment provides a composite plant essential oil mixture, which is different from Example 1 in that: Tween-20 is used to replace the Tween-80, and the rest is the same as Example 1.

[0057] Comparative Example 2

[0058] This embodiment provides a composite plant essential oil mixture, which is different from Example 1 in that: Span-80 is used to replace the Tween-80, and the rest is the same as Example 1.

[0059] Comparative Example 3

[0060] This embodiment provides a composite plant essential oil mixture, which is different from Example 1 in that: propylene glycol is used to replace the absolute ethanol, and the rest is the same as Example 1.

[0061] Comparative Example 4

[0062] This embodiment provides a composite plant essential oil mixture, which is different from Example 1 in that: glycerol is used to replace the absolute ethanol, and the rest is the same as Example 1.

[0063] Examples 1-4 and Comparative Examples 1-4 were mixed according to the formula and observed, and the observation results shown in Table 1 were obtained. Among them, a centrifugation test at 5000 rpm for 30 min was carried out on the formed clear system, the low-temperature treatment temperature was 4 ± 1 °C, the high-temperature treatment temperature was 60 ± 1 °C, and the treatment time was 30 min for both.

[0064] Table 1 shows the observation results when Examples 1-4 and Comparative Examples 1-4 were mixed according to the formula

[0065]

[0066] From the above observation results, it can be seen that only by using specific surfactants and co-surfactants and controlling the mass ratio of the two within an appropriate range can a stable composite plant essential oil microemulsion be obtained.

[0067] Furthermore, microemulsion stability tests were carried out on Examples 1-4, and the specific methods and results are as follows:

[0068] (1) Dilution stability test of composite plant essential oil microemulsion

[0069] This test is used to study the dilution stability test of the composite plant essential oil microemulsion.

[0070] Investigation method: 1 mL of the composite microemulsions prepared in Examples 1-4 were respectively diluted 20 times with tap water, stirred evenly, allowed to stand for 1 h, and the color, and whether there was precipitation and floating oil were observed and recorded.

[0071] Table 2 shows the observation results of the dilution stability test of Examples 1-4

[0072]

[0073]

[0074] From the above test results, it can be seen that after Examples 1-4 were diluted 20 times and allowed to stand for 1 h, Examples 1 and 4 were milky white, and Examples 2 and 3 were transparent and colorless, and there was no oil separation or stratification, indicating that the emulsion was still relatively stable after dilution. And when polyoxyethylene castor oil 360 accounted for the main surfactant, the dilution stability of this microemulsion was better.

[0075] (2) Stability of composite plant essential oil microemulsion under different temperature conditions

[0076] This test is used to study the stability of the composite plant essential oil microemulsion under different temperature conditions.

[0077] The above Examples 1-4 were respectively packed in small bottles with good sealing performance and placed at low temperature (4±1°C), normal temperature (25±2°C), high temperature (40±1°C) and high temperature (60±1°C) for 1 month and 6 months, and whether there was stratification or turbidity was observed. The test results are as follows:

[0078] Table 3 shows the test results of the composite plant essential oil microemulsion prepared in Examples 1-4 under the test conditions for 1 month

[0079] Low temperature (4±1°C) Normal temperature (25±2°C) High temperature (40±1°C) High temperature (60±1°C) Example 1 Slightly solidified Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Example 2 Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Example 3 Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Example 4 Slightly solidified Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified

[0080] Table 4 shows the test results of the composite plant essential oil microemulsion prepared in Examples 1-4 under the test conditions for 6 months

[0081] Low temperature (4±1°C) Normal temperature (25±2°C) High temperature (40±1°C) High temperature (60±1°C) Example 1 Slightly solidified Transparent and non - stratified Transparent and non - stratified Stratified, turbid after mixing Example 2 Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Example 3 Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Transparent and non - stratified Example 4 Slightly solidified Transparent and non - stratified Transparent and non - stratified Stratified, turbid after mixing

[0082] As can be seen from Table 3-4, Examples 1 and 4 will slightly solidify under low temperature (4±1°C) conditions, showing opacity, but will become transparent after being placed for a period of time. There will be a layering phenomenon after being placed at high temperature (60±1°C) for 6 months, and it will still be turbid after being mixed evenly. Examples 2 and 3 remain transparent and non-layered after being placed at low temperature (4±1°C), normal temperature (25±2°C), high temperature (40±1°C) and high temperature (60±1°C) for 1 month and 6 months, indicating that the microemulsion system is relatively stable. The above shows that the high and low temperature stability of this microemulsion with polyoxyl castor oil 360 as the main surfactant component is relatively good.

[0083] (3) Stability experiment of active ingredients of compound plant essential oil microemulsion at different temperatures

[0084] This test is used to study the stability of the active ingredients of the compound plant essential oil microemulsion at different temperatures.

[0085] Investigation method: Example 2 was selected for the test. Example 2 was dispensed into small bottles with good sealing performance and placed at low temperature (4±1°C), normal temperature (25±2°C) and high temperature (40±1°C) for 6 months. Samples were taken at 0 day, 3 months and 6 months to detect the contents of the main components, oregano oil and cinnamaldehyde.

[0086] Table 5 shows the results of the stability of the active ingredients of the compound plant essential oil microemulsion prepared in Example 2 at different temperatures

[0087]

[0088] As can be seen from Table 5, when Example 2 was placed at low temperature (4±1°C), normal temperature (25±2°C) and high temperature (40±1°C) for 6 months, the main component oregano oil showed an upward trend, which may be related to the volatilization of some excipients in this liquid preparation; the cinnamaldehyde only decreased by 3.58% after being placed at normal temperature for 6 months and only decreased by 7.75% after being placed at 40°C for 6 months. Previous studies on the stability of cinnamaldehyde microemulsion prepared by the phase inversion emulsification method found that the content of cinnamaldehyde in the microemulsion decreased slowly at 4°C, and 25.5% was lost at the 6th month, and 60.5% of the content was lost at the 6th month at 30°C. Therefore, the compound plant essential oil microemulsion prepared by the present invention can effectively reduce the volatilization of essential oils and extend the storage time of essential oils.

[0089] From the above test results, it can be seen that the composite plant essential oil microemulsion provided by the present invention is a safe, non-toxic, green and environmentally friendly antibiotic alternative product. It has better stability under normal temperature, high and low temperature and other accelerated conditions, can effectively reduce the volatilization of essential oils, and extend the storage time of essential oils. Further, the present invention can also be applied to livestock and poultry drinking water, which can improve animal production performance, antioxidant capacity and promote the development of immune organs. See the following test results for details.

[0090] (4) Application effect of composite plant essential oil microemulsion on mahuang broilers

[0091] This experiment was used to study the application effect of composite plant essential oil microemulsion on mahuang broilers.

[0092] Investigation method: Example 2 was selected for the experiment. A total of 252 healthy 1-day-old mahuang broiler chicks with good health status and uniform body weight were selected and divided into 3 groups (groups A, B and C) according to body weight according to the principle of random grouping design. Each group had 3 replicates, and each replicate had 28 chickens. Group A was the control group, fed with the basal diet; Group B was the low-dose group of Example 5, with 300 mL / t of liquid essential oil in drinking water; Group C was the high-dose group of Example 5, with 700 mL / t of liquid essential oil in drinking water. After fasting and weighing at 21 days, 3 chickens were randomly selected from each replicate for dissection. After sampling, they were placed in an ice box for the next experiment.

[0093] The test results are as follows:

[0094] (a) Effect of composite plant essential oil microemulsion on the growth performance of mahuang broilers at the chick stage (1 - 21 days) Table 4 shows the effect of the composite plant essential oil microemulsion prepared in Example 2 on the growth performance of mahuang broilers at the chick stage (1 - 21 days)

[0095] Group Initial average weight g / head Final average weight g / head Average daily weight gain g / head / d Average daily feed intake g / head / d Feed - to - gain ratio A 35.29±0.09 <![CDATA[226.64±5.16 b > <![CDATA[9.57±0.26 b > 24.07±0.55 2.52±0.01 B 35.30±0.05 <![CDATA[233.43±2.76 ab > <![CDATA[9.91±0.14 ab > 25.38±1.18 2.57±0.16 C 35.20±0.05 <![CDATA[243.27±1.87 a > <![CDATA[10.40±0.09 a > 25.78±0.74 2.48±0.09

[0096] Note: Values with different lowercase superscripts in the same row indicate significant differences (P < 0.05); the same or no superscript indicates no significant differences (P > 0.05). The same applies to the following tables.

[0097] As can be seen from Table 4, at the chick stage (1 - 21 days), compared with the control group, the average daily weight gain of the high-dose essential oil group increased significantly by 8.67% (P < 0.05), the average daily feed intake increased by 7.10% (P > 0.05), and the feed-to-weight ratio decreased by 1.59%.

[0098] (b) Effect of composite plant essential oil microemulsion on serum physiological and biochemical indexes of mahuang broilers at the chick stage (1 - 21 days).

[0099] Table 5 shows the effect of the composite plant essential oil microemulsion prepared in Example 2 on serum physiological and biochemical indexes of mahuang broilers at the chick stage (1 - 21 days)

[0100] Group MDA (nmol / mL) SOD (U / mL) TP (g / L) ALB (g / L) GLB (g / L) A / G A 5.79±0.77 264.56±16.77 31.81±2.83 14.62±1.45 17.19±1.38 0.85±0.02 B 5.43±0.78 266.65±27.68 34.55±1.86 14.51±0.23 20.04±1.62 0.73±0.05 C 5.72±0.03 276.57±6.81 36.46±0.04 17.68±1.35 18.79±1.31 0.95±0.14

[0101] As can be seen from Table 5, during the chick stage (1 - 21d), compared with the control group, the malondialdehyde (MDA) in the essential oil group decreased (P > 0.05), while the total superoxide dismutase (SOD), total protein (TP), albumin (ALB), and globulin (GLB) increased (P > 0.05).

[0102] (c) Effects of the compound plant essential oil microemulsion on the immune organs of yellow - feather broilers at the chick stage (1 - 21d)Table 6 shows the effects of the compound plant essential oil microemulsion prepared in Example 2 on the immune organs of yellow - feather broilers at the chick stage (1 - 21d).

[0103]

[0104]

[0105] As can be seen from Table 6, during the chick stage (1 - 21d), compared with the control group, the immune organ indices of the high - dose essential oil group increased (P > 0.05).

[0106] The above results show that during the chick stage (1 - 21d), adding 700 mL / t of liquid essential oil to the drinking water can increase the feed intake of yellow - feather broilers (P > 0.05), significantly increase the daily weight gain (P < 0.05), and the feed - to - weight ratio decreases by 1.59% (P > 0.05), indicating that adding 700 mL / t of liquid essential oil to the drinking water can significantly improve the growth performance of chicks; adding different doses of essential oil to the drinking water can reduce the MDA content (P > 0.05) and increase the contents of SOD, TP, ALB, and GLB (P > 0.05), indicating that adding different doses of essential oil to the drinking water can, to a certain extent, improve the antioxidant capacity of chicks and enhance the body's protein synthesis metabolism; adding 700 mL / t of liquid essential oil to the drinking water can increase the immune organ indices of the liver, spleen, and bursa of Fabricius in chicks (P > 0.05), indicating that adding 700 mL / t of liquid essential oil to the drinking water can promote the development of the immune organs of chicks and is helpful for enhancing the body's immunity.

[0107] In summary, the compound plant essential oil microemulsion provided by the present invention is a safe, non - toxic, and green environmental protection alternative to antibiotics. It has better stability under normal temperature, high temperature, and low - temperature acceleration conditions, can effectively reduce the volatilization of essential oil, and extend the storage time of essential oil. Moreover, the present invention can be applied to the drinking water of livestock and poultry, which can improve animal production performance, antioxidant capacity, and promote the development of immune organs.

[0108] The above is the preferred embodiment of the invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements are also regarded as the protection scope of the present invention.

Claims

1. A compound plant essential oil microemulsion, characterized in that, Comprising by weight parts: 10 parts - 14 parts of compound plant essential oil, 24 parts - 40.9 parts of surfactant, 8 parts - 18 parts of co-surfactant, 44 parts - 52 parts of pure water; The compound plant essential oil comprises by weight parts: 60 parts - 80 parts of cinnamaldehyde, 20 parts - 40 parts of origanum oil; The HLB value of the surfactant is 13 - 15.5; The surfactant is polyoxyethylene castor oil 360, or the surfactant is Tween - 80 and polyoxyethylene castor oil 360; The co-surfactant is absolute ethanol.

2. The composite plant essential oil microemulsion according to claim 1, characterized in that, The mass ratio of the surfactant to the co-surfactant is (1 - 4):

1.

3. The composite plant essential oil microemulsion according to claim 1, wherein The mass ratio of the cinnamaldehyde to the origanum oil is (6 - 7):(3 - 4).

4. A method for preparing a composite plant essential oil microemulsion according to any one of claims 1-3, characterized in that, Comprising the following steps: Mix 10 parts - 14 parts of compound plant essential oil, 24 parts - 40.9 parts of surfactant, 8 parts - 18 parts of co-surfactant and 44 parts - 52 parts of pure water, and obtain the finished product after stirring evenly; The compound plant essential oil comprises by weight parts: 60 parts - 80 parts of cinnamaldehyde, 20 parts - 40 parts of origanum oil; The HLB value of the surfactant is 13 - 15.

5.

5. Use of the compound plant essential oil microemulsion according to any one of claims 1 - 3 in livestock and poultry drinking water for improving animal production performance, antioxidant capacity and promoting the development of immune organs.

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