A handheld pesticide residue detector, detection method and controller
By combining a handheld pesticide residue detector with a microfluidic detection chip, the system achieves automated and rapid pesticide residue detection, solving the problems of complex operation and low efficiency in existing technologies, and is suitable for on-site testing.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-04-07
- Publication Date
- 2026-03-13
AI Technical Summary
Existing desktop pesticide residue detectors are complicated to operate and inconvenient for on-site testing, while handheld pesticide residue detectors cannot achieve automated liquid transfer and have low testing efficiency.
A handheld pesticide residue detector was designed, which includes a microfluidic detection chip and related modules. Through the cooperation of the boss structure and the sealing unit, the liquid is automatically transferred between the reaction chamber and the detection chamber, and rapid detection is performed using a heating module, an optical detection module and a data display module.
It realizes a one-time, automated pesticide residue detection process in a microfluidic detection chip, which improves detection efficiency, is suitable for rapid on-site detection, and the device is small and portable.
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Figure CN116577290B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to, but is not limited to, the field of data processing technology, and particularly to a handheld pesticide residue detector, detection method, and controller. Background Technology
[0002] Pesticide residue detection technologies include enzyme inhibition methods, which work by detecting pesticide residues based on the inhibition of enzyme activity by pesticides. Currently, there are two main technical solutions using enzyme inhibition methods: The first is a benchtop pesticide residue detector, which mainly uses a motor to provide centrifugal force to transfer the sample from the reaction area to the detection area, thereby realizing the reaction process. However, benchtop pesticide residue detection processes are complex and require highly skilled personnel, making them inconvenient for on-site testing in the fields and also unsuitable for use in ordinary households. The second is a handheld pesticide residue detector, which manually transfers the sample from the reaction area to the detection area, making automated transfer impossible and resulting in lower detection efficiency. Summary of the Invention
[0003] The following is an overview of the subject matter described in detail herein. This overview is not intended to limit the scope of the claims.
[0004] The main objective of this invention is to provide a handheld pesticide residue detector, detection method, and controller that can meet the needs of on-site testing and improve the efficiency of pesticide residue detection.
[0005] In a first aspect, embodiments of the present invention provide a handheld pesticide residue detector for detecting a liquid to be detected in a microfluidic detection chip. The microfluidic detection chip includes a chip body, which includes a reaction chamber, a detection chamber, and an exhaust chamber connected in sequence. The exhaust chamber is sealed by a sealing unit to prevent gas from flowing out. A boss structure is provided in the flow channel between the reaction chamber and the detection chamber. The boss structure is used to prevent the liquid to be detected from flowing from the reaction chamber into the detection chamber when the exhaust chamber is sealed.
[0006] The handheld pesticide residue detector includes a chip slot for placing the chip body, a heating module, a push rod module, an optical detection module, a data display module, and a controller. The controller is connected to the heating module, the push rod module, the optical detection module, and the data display module. The heating module is used to heat the reaction chamber. The push rod module is used to puncture the sealing unit to allow the liquid to be tested to flow into the detection chamber through the protrusion structure. The optical detection module is used to detect the liquid to be tested in the detection chamber to obtain pesticide residue information. The data display module is used to display inhibition rate information, which is obtained from the pesticide residue information.
[0007] In one embodiment, the flow channel includes a first flow channel region and a second flow channel region connected in sequence. The starting end of the first flow channel region is connected to the reaction chamber. The boss structure is disposed at the end of the first flow channel region and is higher than the lowest position of the first flow channel region. The starting end of the second flow channel region is connected to the end of the first flow channel region. The end of the second flow channel region is disposed near the bottom of the detection chamber. The flow channel area of the first flow channel region is larger than the flow channel area of the second flow channel region.
[0008] In one embodiment, the exhaust chamber of the microfluidic detection chip includes an exhaust unit, the exhaust unit includes an exhaust pipe and an exhaust port communicating with the exhaust pipe, the exhaust port is disposed on the first side, the exhaust pipe is disposed in the exhaust chamber and perpendicular to the first side, the length of the exhaust pipe is less than the distance between the first side and the second side, and the sealing unit is a sealing membrane, the sealing membrane is used to seal the exhaust port to prevent the gas in the detection chamber and the exhaust chamber from communicating with the atmosphere outside the chip body;
[0009] The push rod module includes a drive unit and a needle connected to the drive unit. The diameter of the needle is smaller than the diameter of the exhaust pipe. The drive unit controls the needle to move toward the sealing membrane to pierce the sealing membrane. After piercing the sealing membrane, the needle extends into the exhaust pipe.
[0010] In one embodiment, the driving unit includes a push rod unit, a spring unit, and an electromagnet unit. The needle is disposed on the push rod unit. By controlling the magnetic force of the electromagnet unit, the push rod unit pushes the spring unit, causing the needle to move toward the sealing membrane to pierce the sealing membrane.
[0011] In one embodiment, a detection area is provided on the side wall of the chip body, the detection area is provided corresponding to the detection chamber, the detection area has a concave structure, the detection area includes a first detection area and a second detection area corresponding to the first detection area, the first detection area is provided on a first side of the chip body, the second detection area is provided on a second side of the chip body, the depth of the concave structure of the first detection area is less than the thickness of the first side, and the depth of the concave structure of the second detection area is less than the thickness of the second side;
[0012] The optical detection module includes a light-emitting element and a light-collecting element. The light-emitting element is set in the first detection area, and the light-collecting element is set in the second detection area. The diameter of the light spot emitted by the light-emitting element is smaller than the diameter of the first detection area.
[0013] In one embodiment, the heating module includes a first heating element and a second heating element. One side of the first heating element is disposed in close contact with the first side surface, and the other side is in contact with the heat insulation material. One side of the second heating element is disposed in close contact with the second side surface, and the other side is in contact with the heat insulation material.
[0014] In a first aspect, embodiments of the present invention provide a method for detecting pesticide residues, applied to the controller of the handheld pesticide residue detector described in the first aspect, the method comprising:
[0015] Receive detection command;
[0016] The heating module is controlled to heat according to the detection command;
[0017] After a first preset time, the push rod module is activated to puncture the sealing unit;
[0018] The optical detection module is controlled to emit light, which is directed toward the liquid to be detected in the detection chamber, and pesticide residue information is collected through the optical detection module.
[0019] After a second preset time, the collected pesticide residue information is processed to obtain inhibition rate information;
[0020] The inhibition rate information is sent to the data display module.
[0021] In one embodiment, the chip slot is provided with a detection trigger unit, and before receiving the detection command, the method further includes:
[0022] Obtain the trigger signal of the detection trigger unit;
[0023] Based on the trigger signal, a reception permission message is generated, which indicates that a reception detection command is permitted.
[0024] Thirdly, embodiments of the present invention provide a controller, a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to implement the pesticide residue detection method described in the second aspect.
[0025] Fourthly, embodiments of the present invention provide a computer storage medium storing a computer program thereon, which, when executed by a processor, implements the steps of the pesticide residue detection method described in the second aspect.
[0026] The beneficial effects of this invention include: a handheld pesticide residue detector for detecting a liquid to be tested in a microfluidic detection chip. The microfluidic detection chip includes a chip body, which includes a reaction chamber, a detection chamber, and an exhaust chamber connected in sequence. The exhaust chamber is sealed by a sealing unit to prevent gas from flowing out. A boss structure is provided in the flow channel between the reaction chamber and the detection chamber. The boss structure is used to prevent the liquid to be tested from flowing from the reaction chamber into the detection chamber when the exhaust chamber is sealed. The handheld pesticide residue detector includes a chip slot for placing the chip body, a heating module, a push rod module, an optical detection module, a data display module, and a controller. The controller is connected to the heating module, the push rod module, the optical detection module, and the data display module. The heating module is used to heat the reaction chamber. The push rod module is used to puncture the sealing unit to allow the liquid to be tested to flow into the detection chamber through the boss structure. The optical detection module is used to detect the liquid to be tested in the detection chamber to obtain pesticide residue information. The data display module is used to display inhibition rate information, which is obtained from the pesticide residue information. In this embodiment, when the liquid to be tested needs to be detected, a sealed microfluidic detection chip is first placed in the chip slot, and the liquid to be tested is added to the reaction chamber. As the amount of liquid to be tested flowing into the reaction chamber increases, the gas in the reaction chamber enters the detection chamber through the flow channel between the reaction chamber and the detection chamber. Since the detection chamber and the exhaust chamber are in a sealed state, the pressure difference between the detection chamber and the exhaust chamber is higher than the pressure in the reaction chamber. At this time, the pressure difference between the detection chamber and the exhaust chamber exerts a force on the liquid to be tested in the reaction chamber. Moreover, since a boss structure is provided in the flow channel, under the combined action of the pressure difference and the boss structure, even when the exhaust chamber is sealed, it is possible to... To prevent the liquid to be tested from flowing into the detection chamber from the reaction chamber, the heating module is then controlled to heat the reaction chamber, ensuring the liquid to be tested reacts fully within it. After the liquid to be tested has completed its reaction in the reaction chamber, the push rod module in the handheld pesticide residue detector punctures the sealing unit in the exhaust chamber, reducing the pressure difference between the detection chamber and the exhaust chamber. Without the pressure exerted on the liquid to be tested, it flows into the detection chamber through the boss structure. The optical detection module then detects the liquid in the detection chamber to obtain pesticide residue information. The controller then calculates the inhibition rate based on the pesticide residue information and displays the inhibition rate information through the data display module.By designing the protrusion structure, flow channel, and chip sealing structure of the microfluidic detection chip, and in conjunction with the heating module, push rod module, and optical detection module designed for the microfluidic detection chip structure in a handheld pesticide residue detector, the entire detection process can be completed in the microfluidic detection chip in one go and automatically. That is, in the detection process of this embodiment, with the effective cooperation of the handheld pesticide residue detector, the liquid to be tested in the microfluidic detection chip reacts fully and is automatically transferred, thereby effectively realizing the rapid detection of the liquid to be tested and improving the detection efficiency.
[0027] Other features and advantages of the invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of the invention may be realized and obtained by means of the structures particularly pointed out in the description, claims, and drawings. Attached Figure Description
[0028] Figure 1 This is a schematic diagram of the structure of a handheld pesticide residue detector provided in one embodiment of the present invention;
[0029] Figure 2 This is a schematic diagram of the structure of a microfluidic detection chip provided in one embodiment of the present invention;
[0030] Figure 3 This is an internal plan view of a microfluidic detection chip provided in one embodiment of the present invention;
[0031] Figure 4 This is an internal schematic diagram of a microfluidic detection chip provided in one embodiment of the present invention;
[0032] Figure 5 This is a schematic diagram showing the size settings of a microfluidic detection chip provided in one embodiment of the present invention;
[0033] Figure 6 This is a schematic diagram of the first side plan of a microfluidic detection chip provided in an embodiment of the present invention;
[0034] Figure 7 This is a cross-sectional schematic diagram of a microfluidic detection chip provided in one embodiment of the present invention;
[0035] Figure 8 This is a structural breakdown diagram of a handheld pesticide residue detector provided in one embodiment of the present invention;
[0036] Figure 9 This is a flowchart of a pesticide residue detection method applied to a handheld pesticide residue detector according to an embodiment of the present invention;
[0037] Figure 10 This is a schematic diagram of a controller for a handheld pesticide residue detector provided in one embodiment of the present invention.
[0038] Figure label:
[0039] Chip slot 10, heating module 20, push rod module 30, optical detection module 40, data display module 50, and buttons 60;
[0040] 31. Needle; 32. Push rod unit; 33. Spring unit; 34. Electromagnet unit;
[0041] Light-emitting element 41, light-collecting element 42;
[0042] Sample inlet 100, reaction chamber 200, detection chamber 300, exhaust chamber 400, sealing unit 500, boss structure 600;
[0043] Flow channel 2300, first flow channel region 2310, second flow channel region 2320;
[0044] Exhaust passage 3400, first passage area 3410, second passage area 3420, third passage area 3430;
[0045] Exhaust pipe 411, exhaust port 412, air inlet 413;
[0046] The error-proof structure 700, the first error-proof unit 710, and the second error-proof unit 720;
[0047] First side 810, second side 820, third side 830, fourth side 840, fifth side 850, sixth side 860;
[0048] Testing area 900, first testing area 910, second testing area 920. Detailed Implementation
[0049] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.
[0050] It should be noted that although functional modules are divided in the device schematic diagram and a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than the module division in the device or the order in the flowchart. The terms "first," "second," etc., in the specification, claims, or the aforementioned drawings are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence.
[0051] Among related technologies, pesticide residue detection technologies include enzyme inhibition methods. The principle is based on the inhibition of enzyme activity by pesticides to detect pesticide residues. Currently, there are two main technical solutions using enzyme inhibition methods: The first is a benchtop pesticide residue detector, which mainly uses a motor to provide centrifugal force to transfer the sample from the reaction area to the detection area, thereby realizing the reaction process. However, the benchtop pesticide residue detection process is complex and requires highly skilled personnel, making it inconvenient for on-site testing in the fields and also not suitable for use in ordinary people's homes. The second is a handheld pesticide residue detector, which manually transfers the sample from the reaction area to the detection area, making automated transfer impossible and resulting in lower detection efficiency.
[0052] To address the aforementioned problems, this invention provides a handheld pesticide residue detector for detecting liquids in a microfluidic detection chip. The microfluidic detection chip includes a chip body, which comprises a reaction chamber, a detection chamber, and an exhaust chamber connected sequentially. The exhaust chamber is sealed by a sealing unit to prevent gas from flowing out. A boss structure is provided in the flow channel between the reaction chamber and the detection chamber. This boss structure prevents the liquid to be detected from flowing from the reaction chamber into the detection chamber when the exhaust chamber is sealed. The handheld pesticide residue detector includes a chip slot for placing the chip body, a heating module, a push rod module, an optical detection module, a data display module, and a controller. The controller is connected to the heating module, the push rod module, the optical detection module, and the data display module. The heating module heats the reaction chamber. The push rod module punctures the sealing unit to allow the liquid to be detected to flow into the detection chamber through the boss structure. The optical detection module detects the liquid in the detection chamber to obtain pesticide residue information. The data display module displays inhibition rate information, which is obtained from the pesticide residue information.
[0053] In this embodiment, when the liquid to be tested needs to be detected, a sealed microfluidic detection chip is first placed in the chip slot, and the liquid to be tested is added to the reaction chamber. As the amount of liquid to be tested flowing into the reaction chamber increases, the gas in the reaction chamber enters the detection chamber through the flow channel between the reaction chamber and the detection chamber. Since the detection chamber and the exhaust chamber are in a sealed state, the pressure difference between the detection chamber and the exhaust chamber is higher than the pressure in the reaction chamber. At this time, the pressure difference between the detection chamber and the exhaust chamber exerts a force on the liquid to be tested in the reaction chamber. Moreover, since a boss structure is provided in the flow channel, under the combined action of the pressure difference and the boss structure, even when the exhaust chamber is sealed, it is possible to... To prevent the liquid to be tested from flowing into the detection chamber from the reaction chamber, the heating module is then controlled to heat the reaction chamber, ensuring the liquid to be tested reacts fully within it. After the liquid to be tested has completed its reaction in the reaction chamber, the push rod module in the handheld pesticide residue detector punctures the sealing unit in the exhaust chamber, reducing the pressure difference between the detection chamber and the exhaust chamber. Without the pressure exerted on the liquid to be tested, it flows into the detection chamber through the boss structure. The optical detection module then detects the liquid in the detection chamber to obtain pesticide residue information. The controller then calculates the inhibition rate based on the pesticide residue information and displays the inhibition rate information through the data display module. By designing the protrusion structure, flow channel, and chip sealing structure of the microfluidic detection chip, and in conjunction with the heating module, push rod module, and optical detection module designed specifically for the microfluidic detection chip structure in the handheld pesticide residue detector, the entire detection process can be completed automatically and in one go within the microfluidic detection chip. Specifically, in the detection process of this embodiment, with the effective cooperation of the handheld pesticide residue detector, the test liquid in the microfluidic detection chip fully reacts and undergoes automated transfer, thereby effectively achieving rapid detection of the test liquid and improving detection efficiency. This invention effectively solves the problems of traditional pesticide residue detectors being bulky, complex in operation, time-consuming, and unable to meet the needs of rapid on-site detection. The integrated handheld rapid pesticide residue detector and microfluidic detection chip involved in this invention integrate sample pretreatment, sample detection, and result reading into one unit. It is small in size, easy to carry, and powered by a rechargeable battery, meeting the needs of on-site detection and greatly improving detection efficiency.
[0054] The embodiments of the present invention will be further described below with reference to the accompanying drawings.
[0055] like Figure 1 As shown, where Figure 1This is a schematic diagram of the structure of a handheld pesticide residue detector according to an embodiment of this application. The handheld pesticide residue detector includes a chip slot 10 for placing the chip body, a heating module 20, a push rod module 30, an optical detection module 40, a data display module 50, and a controller. The controller is connected to the heating module 20, the push rod module 30, the optical detection module 40, and the data display module 50. The heating module 20 is used to heat the reaction chamber. The push rod module 30 is used to puncture the sealing unit to allow the liquid to be tested to flow into the detection chamber through the boss structure. The optical detection module 40 is used to detect the liquid to be tested in the detection chamber to obtain pesticide residue information. The data display module 50 is used to display inhibition rate information, which is obtained from the pesticide residue information.
[0056] like Figure 2 As shown, where Figure 2 This is a schematic diagram of the structure of a handheld pesticide residue detector according to an embodiment of this application. The handheld pesticide residue detector includes a chip body, which includes a sample inlet 100, a reaction chamber 200, a detection chamber 300, and an exhaust chamber 400. The reaction chamber 200 is connected to the sample inlet 100, the detection chamber 300 is connected to the reaction chamber 200 through a flow channel 2300, and the exhaust chamber 400 is connected to the detection chamber 300 through an exhaust channel 3400. The exhaust chamber 400 is sealed by a sealing unit 500 to prevent gas from flowing out of the exhaust chamber 400. A boss structure 600 is provided in the flow channel 2300. The boss structure 600 is used to prevent the liquid to be tested from flowing from the reaction chamber 200 into the detection chamber 300 when the exhaust chamber 400 is sealed. When the sealing unit 500 is punctured, the liquid to be tested flows into the detection chamber 300 through the boss structure 600.
[0057] exist Figures 1 to 2In the combined technical solution, when the liquid to be tested needs to be tested, the sealed microfluidic detection chip is first placed in the chip groove 10, and then the liquid to be tested enters the reaction chamber 200 through the injection port 100. As the amount of liquid to be tested flowing into the reaction chamber 200 increases, the gas in the reaction chamber 200 enters the detection chamber 300 through the flow channel 2300 between the reaction chamber 200 and the detection chamber 300. Since the detection chamber 300 and the exhaust chamber 400 are sealed... In this state, the pressure difference between the detection chamber 300 and the exhaust chamber 400 is higher than the pressure in the reaction chamber 200. At this time, the pressure difference between the detection chamber 300 and the exhaust chamber 400 exerts a force on the liquid to be tested in the reaction chamber 200. Furthermore, due to the presence of the boss structure 600 in the flow channel 2300, the combined effect of the pressure difference and the boss structure 600 prevents the liquid to be tested from flowing from the reaction chamber 200 into the detection chamber when the exhaust chamber 400 is sealed. The test liquid is kept in the reaction chamber 200 by the control module 30. The heating module 20 is then used to heat the reaction chamber 200, allowing the test liquid to react fully in the reaction chamber 200. After the test liquid has reacted in the reaction chamber 200 (for example, the first preset time for the test liquid to react in the reaction chamber 200 can be set according to the amount of test liquid and reactant), the push rod module 30 in the handheld pesticide residue detector is then used to puncture the sealing unit 500 in the exhaust chamber 400, reducing the pressure difference between the test chamber 300 and the exhaust chamber 400. In the absence of pressure, the test liquid flows into the test chamber 300 through the boss structure 600. The optical detection module 40 is then used to detect the test liquid in the test chamber 300 to obtain pesticide residue information. The controller then calculates the inhibition rate information based on the pesticide residue information and displays the inhibition rate information through the data display module 50. By designing the protrusion structure 600, flow channel 2300, and chip sealing structure of the microfluidic detection chip, and in conjunction with the heating module 20, push rod module 30, and optical detection module 40 designed for the microfluidic detection chip structure in a handheld pesticide residue detector, the entire detection process can be completed in the microfluidic detection chip in one go and automatically. That is, in the detection process of this embodiment, with the effective cooperation of the handheld pesticide residue detector, the liquid to be tested in the microfluidic detection chip reacts fully and is automatically transferred, thereby effectively realizing the rapid detection of the liquid to be tested and improving the detection efficiency.
[0058] It should be noted that the sealing unit 500 can be a sealing film or an elastic plug; this embodiment does not specifically limit its application. The sealing unit 500 can be a part of the exhaust chamber 400, which is already pasted onto the exhaust chamber during the production process, or it can be independent and pasted onto the exhaust chamber only when the user uses the handheld pesticide residue detector to achieve the purpose of sealing; this embodiment does not specifically limit its application.
[0059] It should be noted that the height of the boss structure 600 is set such that the pressure of the liquid to be tested cannot exceed the pressure of the air in the detection chamber 300 acting on the liquid to be tested, so that when the exhaust chamber 400 is sealed, the boss structure 600 can prevent the liquid to be tested from flowing from the reaction chamber 200 into the detection chamber 300.
[0060] In some alternative embodiments, from Figure 3 , Figure 4 As can be seen, the flow channel 2300 between the reaction chamber 200 and the detection chamber 300 forms a U-shaped channel through the provided boss structure 600. Specifically, the flow channel 2300 includes a first flow channel region 2310 and a second flow channel region 2320 connected in sequence. The starting end of the first flow channel region 2310 is connected to the reaction chamber 200, and the boss structure 600 is disposed on the end of the first flow channel region 2310. The boss structure 600 is higher than the lowest position of the first flow channel region 2310. The starting end of the second flow channel region 2320 is connected to the end of the first flow channel region 2310, and the end of the second flow channel region 2320 is disposed near the bottom of the detection chamber 300. When the liquid to be tested flows into the reaction chamber 200 through the inlet 100, the gas in the reaction chamber 200 flows into the detection chamber 300 through the flow channel 2300. Since the exhaust chamber 400, which is connected to the detection chamber 300, is in a sealed state, as the amount of liquid to be tested increases, the gas density in the detection chamber 300 and the exhaust chamber 400 continuously increases, thereby increasing the gas pressure in the detection chamber 300 and the exhaust chamber 400, which is higher than the gas pressure in the reaction chamber 200. The pressure difference between the detection chamber 300 and the reaction chamber 200 will have a reaction force on the liquid to be tested. Furthermore, due to the presence of the boss structure 600, which increases the resistance of the flow channel 2300, the pressure on the liquid to be tested in the reaction chamber 200 is the same as the pressure in the flow channel 2300, thus allowing the liquid to be tested to remain in the reaction chamber 200. That is, when the exhaust chamber 400 is sealed, the liquid to be tested can fully react with the enzyme reagent filled in the reaction chamber 200.
[0061] In some alternative embodiments, refer to Figure 4The second flow channel region 2320 of the flow channel 2300 is in the shape of a straight pipe. The second flow channel region 2320 of the flow channel 2300 is perpendicular to the bottom plane of the detection chamber 300. Through the design of the second flow channel region 2320 of the flow channel 2300, the liquid to be tested can flow into the detection chamber 300 quickly, and through the flood discharge effect, the liquid to be tested in the first flow channel region 2310 can also be brought into the second flow channel region 2320.
[0062] In some optional embodiments, the flow area of the first flow channel region 2310 is larger than that of the second flow channel region 2320, that is, the flow channel 2300 is set as a variable diameter microchannel. The design of the boss structure 600 combined with the variable diameter microchannel has the effect of increasing the local resistance of the flow channel 2300, which enables the liquid to be tested to flow more smoothly and efficiently.
[0063]
[0064] The pressure on the reaction chamber 200 side is the same as the pressure in the flow channel 2300, allowing the liquid to be tested to remain in the reaction chamber 200. This ensures that, with the exhaust chamber 400 sealed, the liquid to be tested can fully react with the enzyme reagent packed in the reaction chamber 200. Furthermore, the flow area of the first flow channel region 2310 of the variable diameter microchannel is larger than that of the second flow channel region 2320, resulting in a higher flow velocity of the liquid in the second flow channel region 2320 compared to the first flow channel region 2310. This allows for better flood control, enabling the liquid to be tested in the first flow channel region 2310 to be carried into the second flow channel region 2320.
[0065] Specifically, refer to Figure 5 The height h2 of the 600-degree boss structure can be calculated based on the following relationships, as follows:
[0066] Formula (1):
[0067] Where P0 is the local atmospheric pressure, V1 is the volume of the reaction chamber (200), V2 —The total volume of the detection chamber 300, the exhaust chamber 400, and the exhaust passage 3400;
[0068] ΔP1—Local resistance loss of the test liquid through section 1, which is related to the length L1 of the reaction chamber 200, the area S1 of the flow channel 2300 at the beginning of the first flow channel region 2310, the local flow velocity, the density of the test liquid, and the surface roughness of the material.
[0069] ΔP2—Local resistance loss of the measured liquid through section 2, which is related to the length L2 of the detection chamber 300, the distance h1 between the starting end and the lowest point of the first flow channel region 2310, the local flow velocity, and the surface roughness of the material.
[0070] ΔP3—The local resistance loss of the measured liquid through section 3 is related to the flow area S2 at the end of the first flow channel region 2310, the area S1 of the flow channel 2300 at the beginning of the first flow channel region 2310, the height h2 of the boss structure 600, the local flow velocity, and the surface roughness of the material.
[0071] ΔP4—The local resistance loss of the measured liquid through section 4 is related to the flow area S3 of the second flow channel region 2320, the length L2 of the detection chamber 300, the local flow velocity, and the surface roughness of the material.
[0072] ΔP5—The local resistance loss of the measured liquid through section 5 is related to the airway area S4 of the second channel region 3420, the length L2 of the detection chamber 300, the local flow velocity, and the surface roughness of the material.
[0073] ΔP6—The local resistance loss of the measured liquid through section 6 is related to the air passage area S4 of the second channel region 3420, the length L4 of the exhaust chamber 400, the local flow velocity, and the surface roughness of the material.
[0074] ΔP′1——The friction loss of the test liquid through channel 1, which is related to the length h3 of the second flow channel region 2320, the flow area S3 of the second flow channel region 2320, the local flow velocity, and the viscosity coefficient of the test liquid;
[0075] ΔP′2——The friction loss of the test liquid through channel 2, which is related to the length L3 of the first channel region 3410, the air passage area S4 of the second channel region 3420, the length h4 of the second channel region 3420, the local flow velocity, and the viscosity coefficient of the test liquid.
[0076] Formula (2):
[0077]
[0078] Where H is the liquid level, ρ is the density of the liquid being tested, and g is the local gravity proportionality constant.
[0079] By substituting the parameters of formulas (1) and (2) into the calculation, the height h2 can be obtained.
[0080] In some alternative embodiments, refer to Figure 3 , Figure 4The microfluidic detection chip is made of PC plastic injection molding, and its volume can be set to 30mm×26mm×5mm. It mainly includes two chambers: the upper chamber is the reaction chamber and the lower chamber is the detection chamber. Each chamber is pre-embedded with lyophilized reagents. The volume of the reaction chamber 200 on the microfluidic detection chip is about 750 microliters. A sample inlet 100 is opened at the top of the reaction chamber 200. This inlet is used to fill lyophilized reagent beads (the particle size of the lyophilized reagent beads is about 2.0-3.5mm). This embodiment does not specifically limit the size of the beads.
[0081] In some alternative embodiments, refer to Figure 3 , Figure 4 The reaction chamber 200 has a funnel-shaped structure, and its tip 210 is connected to the flow channel 2300. The liquid to be tested can flow more effectively into the flow channel 2300 between the reaction chamber 200 and the detection chamber 300 through the converging effect of the tip 210. The funnel-shaped design of the reaction chamber 200 facilitates the complete flow of the liquid to be tested and its smooth transfer to the detection chamber 2 through the tip 210, avoiding any residual droplets in the reaction chamber 200.
[0082] In some optional embodiments, the projection of the reaction chamber 200 onto the first side 810 of the chip body is the first projection, the projection of the detection chamber 300 onto the first side 810 is the second projection, and the projection of the exhaust chamber 400 onto the first side 810 is the third projection. The first, second, and third projections do not overlap. By setting three non-overlapping chambers on the first side 810, the chip body can be designed to be thinner and is not affected by the stacking of internal chamber structures.
[0083] In some alternative embodiments, refer to Figure 7A detection area 900 is provided on the side wall of the chip body, corresponding to the detection chamber 300. The detection area 900 has a concave structure. Specifically, the detection area 900 includes a first detection area 910 and a second detection area 920 corresponding to the first detection area 910. The first detection area 910 is located on the first side 810 of the chip body, and the second detection area 920 is located on the second side 820 of the chip body. The depth of the concave structure of the first detection area 910 is less than the thickness of the first side 810, and the depth of the concave structure of the second detection area 920 is less than the thickness of the second side 820. This portion is thinner to improve light transmittance. Furthermore, the projected area of the first detection area 910 on the first side 810 is larger than the area of the light spot emitted by the light-emitting element of the pesticide residue detector, but smaller than the projected area of the first projection. The projected area of the second detection area 920 on the second side 820 is smaller than the projected area of the reaction chamber 200 on the second side 820. The first detection area 910 and the second detection area 920 are arranged concentrically, and both the first detection area 910 and the second detection area 920 are circular with the same diameter. Since the areas of the first detection area 910 and the second detection area 920 are larger than the size of the light spot emitted by the light-emitting element, the concave structure design is to reduce the chip thickness of the detection area 9001, which can effectively allow the light spot emitted by the light-emitting element to pass smoothly through the detection cavity, improve the light spot transmittance, and thus improve the detection accuracy.
[0084] It should be noted that the detection area 9001 can be a concave structure with a diameter of 9 mm and a depth of 1 mm, or it can be a concave structure with a diameter of 10 mm and a depth of 1.2 mm. This embodiment does not make specific limitations on it.
[0085] In some alternative embodiments, refer to Figure 4The exhaust passage 3400 includes a first passage region 3410, a second passage region 3420, and a third passage region 3430 connected in sequence. The air inlet end of the first passage region 3410 is located at the top of the detection chamber 300 and communicates with it. The vertical distance between the connecting ends of the first passage region 3410 and the second passage region 3420 and the bottom of the detection chamber 300 is greater than the vertical distance between the air inlet end of the first passage region 3410 and the bottom of the detection chamber 300. The vertical distance between the connecting ends of the second passage region 3420 and the third passage region 3430 is greater than the vertical distance between the connecting ends of the second passage region 3410 and the bottom of the detection chamber 300. The vertical distance between the connecting end of the third channel region 3410 and the bottom of the detection chamber 300 is greater than the vertical distance between the connecting end of the first channel region 3410 and the second channel region 3420 and the bottom of the detection chamber 300. The outlet end of the third channel region 3430 is located at the top of the exhaust chamber 400 and communicates with the exhaust chamber 400. The vertical distance between the outlet end of the third channel region 3430 and the bottom of the detection chamber 300 is smaller than the vertical distance between the connecting end of the second channel region 3420 and the third channel region 3430 and the bottom of the detection chamber 300. Because the vertical distance between the connecting end of the first channel region 3410 and the second channel region 3420 and the bottom of the detection chamber 300 is greater than the vertical distance between the air inlet end of the first channel region 3410 and the bottom of the detection chamber 300, even if the liquid to be tested quickly passes through the second flow channel region 2320 and flows into the detection chamber 300, the collision between the liquid to be tested and the bottom of the detection chamber 300 will generate a large rebound force, causing some of the liquid to be tested to enter the first channel region 3410. Due to the sloping design of the first channel region 3410, the portion of the liquid to be tested that enters the first channel region 3410 will be affected by the weight of the liquid. The new liquid flows back to the detection chamber 300. Furthermore, since the vertical distance between the connecting end of the second channel region 3420 and the third channel region 3430 and the bottom of the detection chamber 300 is greater than the vertical distance between the connecting end of the first channel region 3410 and the second channel region 3420 and the bottom of the detection chamber 300, the first channel region 3410 and the second channel region 3420 can form a double resistance in the gas channel, making it difficult for the liquid to be tested in the detection chamber 300 to flow to the exhaust chamber 400, effectively reducing the risk of the liquid to be tested flowing out of the chip body, thereby effectively protecting the handheld pesticide residue detector.
[0086] In some alternative embodiments, refer to Figure 7The exhaust chamber 400 includes an exhaust unit 410, which includes an exhaust pipe 411, an exhaust port 412 communicating with the exhaust pipe 411, and an air inlet 413 communicating with the exhaust pipe 411. The exhaust port 412 is located on the first side 810. The exhaust pipe 411 is located inside the exhaust chamber 400 and perpendicular to the first side 810. The length of the exhaust pipe 411 is less than the distance between the first side 810 and the second side 820. The gas in the exhaust chamber 400 enters the exhaust pipe 411 through the air inlet 413 and then exits through the exhaust port 412. The sealing unit 500 is a sealing membrane used to seal the exhaust port 412 to prevent the gas in the detection chamber 300 and the exhaust chamber 400 from communicating with the atmosphere outside the chip body. Because the exhaust pipe 411 is designed to be perpendicular to the first side 810 and has a certain length, even if the liquid to be tested flows into the exhaust chamber when the chip body is tilted or vibrated during use, it will not flow through the exhaust pipe 411 to the exhaust hole 412 and out of the chip body, thus effectively protecting the handheld pesticide residue detector.
[0087] It should be noted that when the sealing unit 500 is a sealing film, the sealing unit 500 can be a thin aluminum film with a thickness of 0.05 mm or a thin adhesive film with a thickness of 0.03 mm. This embodiment does not make specific limitations on it.
[0088] In some alternative embodiments, refer to Figure 6 The chip body includes a foolproof structure 700 disposed on the first side 810, which is a groove structure. Specifically, the foolproof structure 700 includes a first foolproof unit 710 and a second foolproof unit 720. The projection of the first foolproof unit 710 on the first side 810 overlaps with the projection of the flow channel 2300 on the first side 810, and the projection of the second foolproof unit 720 on the first side 810 overlaps with the projection of the gas channel on the first side 810. That is, the foolproof structure 700 does not need to increase the area of the first side 810 of the chip body. With a fixed area of the first side 810, the volume of the reaction chamber 200, the detection chamber 300, and the exhaust chamber 400 can be maximized. This is beneficial for controlling the size of the handheld pesticide residue detector and allows the handheld pesticide residue detector to be designed as a miniature structure.
[0089] It should be noted that the shape of the foolproof structure 700 is "L" shaped, or it can be other irregular shapes. This embodiment does not specifically limit it, as long as it can effectively prevent the chip body from shifting during the detection process.
[0090] In some alternative embodiments, refer to Figure 2 , Figure 4 , Figure 6 , Figure 7The chip body includes a first side 810, a second side 820, a third side 830, a fourth side 840, a fifth side 850, and a sixth side 860. The first side 810 and the second side 820 have the same shape and are arranged parallel to each other. The third side 830 and the fourth side 840 have the same shape and are arranged perpendicular to the first side 810. The fifth side 850 and the sixth side 860 have the same shape, are arranged perpendicular to the first side 810, and are also perpendicular to the fifth side 850. The side length of the bounding rectangle of the first side 810 is the length and width of the chip body. The side length of the bounding rectangle of the third side 830 is the length and height of the chip body. The side length of the bounding rectangle of the fifth side 850 is the width and height of the chip body.
[0091] It should be noted that the length of the chip body is less than or equal to 50mm, the width is less than or equal to 45mm, and the height is less than or equal to 10mm. For example, the size can be set to 30mm×26mm×5mm; another example is 40mm×23mm×7mm. Of course, other dimensions can be set according to actual needs to meet the requirements of small instrument size, low manufacturing cost, and easy portability.
[0092] The working principle of the microfluidic detection chip: Before adding the reaction liquid to the handheld pesticide residue detector, a sealing unit 500 is attached to the exhaust chamber 400, making the end of the handheld pesticide residue detector in a sealed state (the detection chamber 300 and the exhaust chamber 400 are in a sealed state). During the detection process, the liquid to be detected enters the reaction chamber 200 through the inlet port 100. As the amount of liquid to be detected flowing into the reaction chamber 200 increases, the amount of gas in the reaction chamber 200 entering the detection chamber 300 through the flow channel 2300 between the reaction chamber 200 and the detection chamber 300 also increases. Since the detection chamber 300 and the exhaust chamber 400 are in a sealed state, the gas pressure in the detection chamber 300 and the exhaust chamber 400 continuously increases and becomes higher than the gas pressure in the reaction chamber 200. At this time, the gas pressure in the detection chamber 300 and the exhaust chamber 400 exerts a pressure on the liquid to be detected in the reaction chamber 200. The liquid being tested exerts a force, and because the flow channel 2300 has a boss structure 600 that increases the resistance of the flow channel 2300, the liquid being tested, under the combined effect of the pressure difference and the boss structure 600, remains in the reaction chamber 200 and does not flow into the detection chamber 300. This allows the liquid to react fully in the reaction chamber 200. After the liquid has reacted in the reaction chamber 200, the push rod module 30 in the handheld pesticide residue detector can puncture the sealing unit 500 in the exhaust chamber 400, reducing the pressure in both the detection chamber 300 and the exhaust chamber 400. Without the pressure exerted on the liquid, it flows through the boss structure 600 into the detection chamber 300. The sample can be automatically transferred within the handheld pesticide residue detector, enabling rapid detection and improving detection efficiency.
[0093] In addition, such as Figure 8 Another embodiment of the handheld pesticide residue detector shown includes the handheld pesticide residue detector in the above embodiment, and the structure of the handheld pesticide residue detector is the same as... Figure 2-7 The structure and design of the microfluidic pesticide residue detection chip shown are based on Figure 1One embodiment of the handheld pesticide residue detector includes a push rod module comprising a drive unit and a needle 31 connected to the drive unit. The diameter of the needle 31 is smaller than the diameter of the exhaust pipe 411. The drive unit controls the needle 31 to move toward the sealing membrane to pierce the sealing membrane. After piercing the sealing membrane, the needle 31 extends into the exhaust pipe 411. The optical detection module includes a light-emitting element 41 and a light-collecting element 42. The light-emitting element 41 is disposed corresponding to the first detection area 910, and the light-collecting element 42 is disposed corresponding to the second detection area 920. The diameter of the light spot emitted by the light-emitting element 41 is smaller than the diameter of the first detection area 910. The heating module includes a first heating element 21 and a second heating element 22. One side of the first heating element 21 is disposed in close contact with the first side surface, and the other side is in contact with the heat insulation material. One side of the second heating element 22 is disposed in close contact with the second side surface, and the other side is in contact with the heat insulation material.
[0094] It should be noted that the first heating element 21 and the second heating element 22 are arranged with one side in close contact with the side surface and the other side in contact with the heat insulation material, which can effectively prevent heat loss and burns to the surface of the instrument during the heating process. The heating module can be connected to the power supply through a wire or to the battery through the designed contacts.
[0095] In some optional embodiments, the light-emitting element 41 is disposed corresponding to the first detection area 910, and the distance between the light-emitting element 41 and the first detection area 910 is 2-2.5 mm; the light-collecting element 42 is disposed corresponding to the second detection area 920, and the distance between the light-collecting element 42 and the second detection area 920 is 1.5-2 mm.
[0096] In some optional embodiments, the drive unit includes a push rod unit 32, a spring unit 33, and an electromagnet unit 34. The needle 31 is disposed on the push rod unit 32. By controlling the magnetic force of the electromagnet unit 34, the push rod unit 32 pushes the spring unit 33, causing the needle 31 to move toward the sealing film to pierce the sealing film. Then, when the control electromagnet unit 34 loses its magnetic force on the push rod unit 32, the push rod unit 32 returns to its original position under the action of the spring, so that the needle 31 moves from inside the microfluidic detection chip to the outside.
[0097] In some optional embodiments, in order to prevent the needle 31 from damaging the microfluidic detection chip, the length of the needle 31 is smaller than the length of the exhaust pipe 411, the needle 31 is designed parallel to the exhaust pipe 411, and the diameter of the push rod unit 32 is larger than the diameter of the hole. Then, during the process of the needle 31 piercing the sealing film, the depth of the needle 31 piercing into the microfluidic detection chip can be controlled by the limiting effect of the push rod unit 32, so as to prevent the needle 31 from contacting the side of the microfluidic detection chip.
[0098] In some optional embodiments, in order to prevent the needle 31 from damaging the microfluidic detection chip, the minimum length of the compressed spring unit 33 can be set to be less than the length of the exhaust pipe 411. Then, during the process of the needle 31 piercing the sealing film, the limiting effect of the spring unit 33 can control the range of motion of the needle 31, thereby controlling the depth of the needle 31 piercing into the microfluidic detection chip and preventing the needle 31 from contacting the side of the microfluidic detection chip.
[0099] In some optional embodiments, a button 60 is also provided on the handheld pesticide residue detector. This button 60 is used to power on the handheld pesticide residue detector and to start the detection program. For example, pressing and holding the button 60 for more than a time threshold (such as 3 seconds, 4 seconds, or 2.5 seconds, which is not specifically limited in this embodiment) triggers the handheld pesticide residue detector to power on. When powered on, pressing the button 60 once or twice consecutively starts the detection program.
[0100] In some optional embodiments, to prevent the heating module from being accidentally activated and causing dry burning due to the microfluidic detection chip not being placed in the chip slot 10, a detection trigger unit can be set in the chip slot 10. When the microfluidic detection chip is placed in the chip slot 10, the detection trigger unit will be triggered to confirm that the microfluidic detection chip is placed in the chip slot. It should be noted that the detection trigger unit can be an infrared sensor or a physical button, which will generate a trigger signal when the microfluidic detection chip is detected. For example, if the detection trigger unit does not detect the microfluidic detection chip, no trigger signal will be generated, so even when the device is powered on, pressing the button 60 once or twice consecutively will not start the detection program.
[0101] The working principle of the optical detection module: The sample inlet 100 can be filled with lyophilized reagent beads, the reaction chamber 200 can be filled with enzyme reagents, and the detection chamber 300 can be filled with colorimetric reagents. Specifically, the working principle of the optical detection module used in the detection process of the liquid to be tested in the handheld pesticide residue detector is as follows: Organophosphorus and carbamate pesticides have an inhibitory effect on the normal function of cholinesterase, and the inhibition rate is positively correlated with the concentration of pesticides. During detection, the enzyme catalyzes the hydrolysis of the neurotransmitter metabolite (acetylcholine), and the hydrolysis product reacts with the colorimetric reagent to produce a yellow substance. The light spot emitted by the LED light-emitting element 41 of the handheld pesticide residue detector passes through the detection chamber 300, and the light-collecting element 42 of the handheld pesticide residue detector measures the change of its absorbance over time. The data transmission value is used by the control system to calculate the inhibition rate. If the inhibition rate is greater than 50%, the sample to be tested can be determined to be positive; otherwise, it is negative.
[0102] based on Figures 1 to 8 In the embodiment of the handheld pesticide residue detector and the microfluidic detection chip, Figure 9 This embodiment provides a flowchart of a method for detecting pesticide residues. This method is the detection procedure corresponding to the above embodiment, and includes the following steps:
[0103] Step S100: Receive detection command.
[0104] In some optional embodiments, pressing and holding the button turns on the handheld pesticide residue detector. Detection information such as the detection serial number, the name of the person conducting the test, and the testing location can be set. The lyophilized reagent is placed into the reaction chamber of the microfluidic detection chip, and the collected sample to be tested is injected into the reaction chamber. The microfluidic detection chip is placed into the chip slot, the heating module is closed, and the button is pressed to send the detection command. At this time, the controller will receive the detection command.
[0105] Step S200: Control the heating module to heat according to the detection command.
[0106] In some optional embodiments, the controller will control the heating module to heat according to the detection command, for example, setting the reaction temperature to 37°C for 4 minutes, or setting the reaction temperature to 37.5°C for 3.5 minutes. The settings can be made according to the actual situation. This embodiment does not make specific limitations on them, as long as the sample to be tested, the reaction reagent and the enzyme react fully.
[0107] In step S300, after a first preset time, the control push rod module is activated to puncture the sealing unit.
[0108] In some optional embodiments, the first preset time is the heating time of the heating module on the reaction chamber, which can be set according to the actual situation. After the first preset time has elapsed, that is, after the sample to be tested, the reaction reagent and the enzyme have fully reacted, the push rod module is activated to puncture the sealing unit, so that the pressure difference between the detection chamber and the exhaust chamber is reduced. Under the condition of lack of pressure on the liquid to be tested, the liquid to be tested flows into the detection chamber through the boss structure.
[0109] In step S400, the optical detection module is controlled to emit light, which is directed at the liquid to be detected in the detection chamber, and pesticide residue information is collected through the optical detection module.
[0110] In step S500, after a second preset time, the collected pesticide residue information is calculated and processed to obtain inhibition rate information.
[0111] Step S600: The inhibition rate information is sent to the data display module.
[0112] In some optional embodiments, the light spot emitted by the light-emitting element is controlled so that its size is precisely aligned with the concave structure of the first detection area. The light-collecting element collects pesticide residue information through a microfluidic detection chip. The detection time can be set to 2 minutes, or it can be set to 2.5 minutes; this embodiment does not impose a specific limitation and can be set according to actual conditions. The controller calculates and processes the collected pesticide residue information to obtain inhibition rate information and sends the inhibition rate information to the data display module.
[0113] In this embodiment, when the liquid to be tested needs to be detected, a sealed microfluidic detection chip is first placed in the chip slot, and the liquid to be tested is added to the reaction chamber. As the amount of liquid to be tested flowing into the reaction chamber increases, the gas in the reaction chamber enters the detection chamber through the flow channel between the reaction chamber and the detection chamber. Since the detection chamber and the exhaust chamber are in a sealed state, the pressure difference between the detection chamber and the exhaust chamber is higher than the pressure in the reaction chamber. At this time, the pressure difference between the detection chamber and the exhaust chamber exerts a force on the liquid to be tested in the reaction chamber. Moreover, since a boss structure is provided in the flow channel, under the combined action of the pressure difference and the boss structure, even when the exhaust chamber is sealed, it is possible to... To prevent the liquid to be tested from flowing into the detection chamber from the reaction chamber, the heating module is then controlled to heat the reaction chamber, ensuring the liquid to be tested reacts fully within it. After the liquid to be tested has completed its reaction in the reaction chamber, the push rod module in the handheld pesticide residue detector punctures the sealing unit in the exhaust chamber, reducing the pressure difference between the detection chamber and the exhaust chamber. Without the pressure exerted on the liquid to be tested, it flows into the detection chamber through the boss structure. The optical detection module then detects the liquid in the detection chamber to obtain pesticide residue information. The controller then calculates the inhibition rate based on the pesticide residue information and displays the inhibition rate information through the data display module. By designing the protrusion structure, flow channel, and chip sealing structure of the microfluidic detection chip, and in conjunction with the heating module, push rod module, and optical detection module designed specifically for the microfluidic detection chip structure in the handheld pesticide residue detector, the entire detection process can be completed automatically and in one go within the microfluidic detection chip. Specifically, in the detection process of this embodiment, with the effective cooperation of the handheld pesticide residue detector, the test liquid in the microfluidic detection chip fully reacts and undergoes automated transfer, thereby effectively achieving rapid detection of the test liquid and improving detection efficiency. This invention effectively solves the problems of traditional pesticide residue detectors being bulky, complex in operation, time-consuming, and unable to meet the needs of rapid on-site detection. The integrated handheld rapid pesticide residue detector and microfluidic detection chip involved in this invention integrate sample pretreatment, sample detection, and result reading into one unit. It is small in size, easy to carry, and powered by a rechargeable battery, meeting the needs of on-site detection and greatly improving detection efficiency.
[0114] In some optional embodiments, the handheld pesticide residue detector is equipped with a Bluetooth module and / or a Type-C interface, which can transmit or save the detected pesticide residue information to other storage units via wireless Bluetooth or by using the Type-C interface.
[0115] In some optional embodiments, the handheld pesticide residue detector can be turned off by pressing and holding the button after the test is completed.
[0116] In some optional embodiments, the handheld pesticide residue detector is equipped with a detection trigger unit. When the microfluidic detection chip is placed in the chip slot of the handheld pesticide residue detector, the detection trigger unit generates a trigger signal. The controller then receives the trigger signal from the detection trigger unit and generates an allow-to-receive information based on the trigger signal. The allow-to-receive information indicates that the detection command can be received, which can prevent the heating module from being accidentally triggered and dry-burning when the microfluidic detection chip is not placed in the chip slot. In addition, the handheld pesticide residue detector is also equipped with a cover that can be opened and closed by rotation. This cover is used to cover the microfluidic detection chip. The structure of the cover is designed so that the microfluidic detection chip is completely placed in the chip slot 10. During the closing process, the cover can also push the microfluidic detection chip so that it can be completely placed in the chip slot 10, allowing the microfluidic detection chip to move closer to or contact the detection trigger unit, thereby causing the detection trigger unit to generate a trigger signal.
[0117] In some optional embodiments, the handheld pesticide residue detector includes a power module for supplying power to the heating module 20, push rod module 30, optical detection module 40, data display module 50, controller, and detection trigger unit. It should be noted that the power module can be a rechargeable battery or a dry cell battery; this embodiment does not specifically limit its use.
[0118] In addition, such as Figure 10 As shown, one embodiment of the present invention provides a controller 1000 for a handheld pesticide residue detector. The controller 1000 includes a processor 1010 and a memory 1020, wherein the processor 1010 and the memory 1020 can be connected via a bus or other means. Figure 1 Taking the example of a connection between China and Israel via a bus.
[0119] The memory 1020, as a schematic diagram of a system platform architecture for performing a method to improve flash memory reliability provided in one embodiment of the present invention, is a readable storage medium that can be used to store non-transitory software programs and non-transitory computer-executable programs. Furthermore, the memory 1020 may include high-speed random access memory and may also include non-transitory memory, such as at least one disk storage device, flash memory device, or other non-transitory solid-state storage device. In some embodiments, the memory 1020 may optionally include memory remotely located relative to the processor 1010.
[0120] The controller 1000 stores the non-transitory software program and instructions required to implement the method for improving flash memory reliability described in the above embodiments in a memory. When executed by a processor, it performs the method for improving flash memory reliability described in the above embodiments, for example, performing the above-described method. Figure 8 Method steps S100 to S600.
[0121] Furthermore, one embodiment of the present invention provides a computer-readable storage medium storing computer-executable instructions, which, when used to execute the method for improving flash memory reliability of the controller described above, for example, execute the above-described method... Figure 8 Method steps S100 to S600.
[0122] It will be understood by those skilled in the art that all or some of the steps and systems in the methods disclosed above can be implemented as software, firmware, hardware, and suitable combinations thereof. Some or all of the physical components can be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application-specific integrated circuit. Such software can be distributed on a computer-readable medium, which can include computer storage media (or non-transitory media) and communication media (or transient media). As is known to those skilled in the art, the term computer storage media includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information (such as computer-readable instructions, data structures, program modules, or other data). Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disc (DVD) or other optical disc storage, magnetic cartridges, magnetic tape, disk storage or other magnetic storage devices, or any other medium that can be used to store desired information and is accessible to a computer. Furthermore, as is known to those skilled in the art, communication media typically include computer-readable instructions, data structures, program modules, or other data in modulated data signals such as carrier waves or other transmission mechanisms, and may include any information delivery medium.
[0123] The above provides a detailed description of the preferred embodiments of the present invention. However, the present invention is not limited to the above embodiments. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present invention. All such equivalent modifications or substitutions are included within the scope defined by the claims of the present invention.
Claims
1. A handheld pesticide residue detector, characterized in that, This device is used to detect liquids in a microfluidic detection chip. The microfluidic detection chip includes a chip body, which comprises a reaction chamber, a detection chamber, and an exhaust chamber connected in sequence. The exhaust chamber is sealed by a sealing unit to prevent gas from flowing out. A boss structure is provided in the flow channel between the reaction chamber and the detection chamber. The boss structure is used to prevent the liquid to be detected from flowing from the reaction chamber into the detection chamber when the exhaust chamber is sealed. The handheld pesticide residue detector includes a chip slot for placing the chip body, a heating module, a push rod module, an optical detection module, a data display module, and a controller. The controller is connected to the heating module, the push rod module, the optical detection module, and the data display module. The heating module is used to heat the reaction chamber. The push rod module is used to puncture the sealing unit to allow the liquid to be tested to flow into the detection chamber through the protrusion structure. The optical detection module is used to detect the liquid to be tested in the detection chamber to obtain pesticide residue information. The data display module is used to display inhibition rate information, which is obtained from the pesticide residue information. The flow channel includes a first flow channel region and a second flow channel region connected in sequence. The starting end of the first flow channel region is connected to the reaction chamber. The boss structure is disposed at the end of the first flow channel region and is higher than the lowest position of the first flow channel region. The starting end of the second flow channel region is connected to the end of the first flow channel region. The end of the second flow channel region is disposed near the bottom of the detection chamber. The flow channel area of the first flow channel region is larger than the flow channel area of the second flow channel region.
2. The handheld pesticide residue detector according to claim 1, characterized in that, The exhaust chamber of the microfluidic detection chip includes an exhaust unit, which includes an exhaust pipe and an exhaust port communicating with the exhaust pipe. The exhaust port is disposed on a first side of the chip body, and the exhaust pipe is disposed in the exhaust chamber and perpendicular to the first side. The length of the exhaust pipe is less than the distance between the first side and the second side of the chip body. The sealing unit is a sealing membrane, which is used to seal the exhaust port to prevent the gas in the detection chamber and the exhaust chamber from communicating with the atmosphere outside the chip body. The push rod module includes a drive unit and a needle connected to the drive unit. The diameter of the needle is smaller than the diameter of the exhaust pipe. The drive unit controls the needle to move toward the sealing membrane to pierce the sealing membrane. After piercing the sealing membrane, the needle extends into the exhaust pipe.
3. The handheld pesticide residue detector according to claim 2, characterized in that, The drive unit includes a push rod unit, a spring unit, and an electromagnet unit. The needle is disposed on the push rod unit. By controlling the magnetic force of the electromagnet unit, the push rod unit pushes the spring unit, causing the needle to move toward the sealing membrane to pierce the sealing membrane.
4. The handheld pesticide residue detector according to claim 1, characterized in that, A detection area is provided on the side wall of the chip body, the detection area is provided corresponding to the detection chamber, the detection area is a concave structure, the detection area includes a first detection area and a second detection area corresponding to the first detection area, the first detection area is provided on the first side of the chip body, the second detection area is provided on the second side of the chip body, the depth of the concave structure of the first detection area is less than the thickness of the first side, and the depth of the concave structure of the second detection area is less than the thickness of the second side. The optical detection module includes a light-emitting element and a light-collecting element. The light-emitting element is set in the first detection area, and the light-collecting element is set in the second detection area. The diameter of the light spot emitted by the light-emitting element is smaller than the diameter of the first detection area.
5. The handheld pesticide residue detector according to claim 4, characterized in that, The heating module includes a first heating element and a second heating element. One side of the first heating element is in close contact with the first side surface, and the other side is in contact with the heat insulation material. One side of the second heating element is in close contact with the second side surface, and the other side is in contact with the heat insulation material.
6. A method for detecting pesticide residues, characterized in that, The method, applied to the controller of the handheld pesticide residue detector according to any one of claims 1-5, comprises: Receive detection command; The heating module is controlled to heat according to the detection command; After a first preset time, the push rod module is activated to puncture the sealing unit; The optical detection module is controlled to emit light, which is directed toward the liquid to be detected in the detection chamber, and pesticide residue information is collected through the optical detection module. After a second preset time, the collected pesticide residue information is processed to obtain inhibition rate information; The inhibition rate information is sent to the data display module.
7. The pesticide residue detection method according to claim 6, characterized in that, The chip slot is provided with a detection trigger unit, and before receiving the detection command, the method further includes: Obtain the trigger signal of the detection trigger unit; Based on the trigger signal, a reception permission message is generated, which indicates that a reception detection command is permitted.
8. A controller, characterized in that, A memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor, when executing the computer program, implements the pesticide residue detection method according to any one of claims 6 to 7.
9. A computer storage medium having a computer program stored thereon, characterized in that, When executed by a processor, the computer program implements the steps of the pesticide residue detection method according to any one of claims 6 to 7.
Citation Information
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