A laboratory layout structure of a secondary protection barrier of a three-level biosafety laboratory

Abstract

This invention discloses a laboratory layout structure for a secondary protective barrier in a Level 3 biosafety laboratory, comprising: a negative pressure protection zone and a positive pressure auxiliary protection zone. The negative pressure protection zone includes a BSL-3 core experimental area, an ABSL-3 core experimental area, a buffer room, an animal quarantine room, a temporary storage room for supplies, an animal carcass storage room, and a second personnel protective area. The positive pressure auxiliary protection zone includes an animal anteroom, a first preparation and cleaning room, a second preparation and cleaning room, a protective corridor, and a first personnel protective area. A buffer room connects the BSL-3 core experimental area and the ABSL-3 core experimental area. The protective corridor surrounds the negative pressure protection zone on three sides. The entire laboratory layout structure simultaneously provides a secondary protective barrier for both a BSL-3 Level 3 microbial biosafety laboratory and an ABSL-3 Level 3 animal biosafety laboratory, ensuring that harmful biological agents cannot leak out of the laboratory and that the overall cleanliness of the laboratory meets national standards.

Description

Technical Field

[0001] This invention belongs to the field of bioengineering, and specifically relates to a laboratory layout structure for a secondary protective barrier in a biosafety level 3 laboratory. Background Technology

[0002] A Level 3 (P3) biosafety laboratory is a high-level biosafety laboratory. It handles infectious microorganisms that pose a high individual hazard but a low population hazard, meaning they are highly dangerous to humans, animals, plants, or the environment. These microorganisms can cause serious or even fatal diseases in humans through direct contact or aerosol transmission, or are highly pathogenic agents that pose a significant risk to animals, plants, and the environment, such as SARS coronavirus, typhoid bacillus, and rickettsiae. Preventive and treatment measures are usually in place. For example, the applicant disclosed a biosafety laboratory layout structure in patent application CN206769475U, which includes a first P3 core area and a second P3 core area. This laboratory is a Level 3 biosafety laboratory.

[0003] However, existing biosafety level 3 (BSL-3) laboratories still have some shortcomings that need improvement: 1) The first personnel protection zone is flawed in the existing technology. 2) The existing technology lacks a maximum positive pressure protection corridor. 3) The second personnel protection zone is inadequate in the existing technology. 4) The existing technology does not scientifically integrate BSL-3 and ABSL-3 simultaneously. 5) The air pressure gradient design of each auxiliary functional room is unreasonable in the existing technology. 6) The material flow in and out of the laboratory is complex. For example, incoming materials include animals, items, feed, and samples, while outgoing materials include animal carcasses, discarded instruments, and waste. Existing laboratory technologies do not strictly control the breakdown of material flow based on more detailed and specific circumstances.

[0004] The layout of auxiliary rooms within the secondary protective barrier differs, as do the ventilation system pressure settings in each auxiliary room, the overall airflow patterns within the laboratory, and the configuration of the sterilization and disinfection systems. This also affects the frequency of personnel movement across boundaries and the turbulence of airflow near the work surfaces of biosafety cabinets. Overall, this results in variations in the stability of laboratory safety, functionality, comfort, and cleanliness.

[0005] In view of the above, this invention develops a secondary protective barrier technology for biosafety level 3 laboratories to improve the reliability and safety of the secondary protective barrier and promote the advancement of the design and construction of high-level biosafety laboratories in my country. Summary of the Invention

[0006] In order to solve the above problems, the present invention aims to provide a laboratory layout structure for a secondary protective barrier in a biosafety level 3 laboratory.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] This invention provides a laboratory layout structure for a secondary protective barrier in a biosafety level 3 laboratory, characterized by comprising: a negative pressure protection zone and a positive pressure auxiliary protection zone. The negative pressure protection zone includes a BSL-3 core experimental area, an ABSL-3 core experimental area, a buffer room, an animal quarantine room, a temporary storage room for supplies, an animal carcass storage room, and a second personnel protection zone. The positive pressure auxiliary protection zone includes an animal anteroom, a first preparatory cleaning room, a second preparatory cleaning room, a protective corridor, and a first personnel protection zone. A buffer room connects the BSL-3 core experimental area and the ABSL-3 core experimental area. A double-door autoclave leading to the first preparatory cleaning room and a first hydrogen peroxide transfer chamber leading to the BSL-3 core experimental area are respectively provided between the BSL-3 core experimental area and the first preparatory cleaning room. The ABSL-3 core experimental area is connected to the animal quarantine room, the temporary storage room, and the animal carcass storage room. An animal safety receiving station is located between the animal quarantine room and the animal anteroom, leading to the animal quarantine room. A second hydrogen peroxide transfer chamber is located between the temporary storage room and the second preparation and cleaning room, leading to the temporary storage room. A walking-type double-door autoclave is installed between the animal carcass storage room and the second preparation and cleaning room. The first preparation and cleaning room, the animal anteroom, and the second preparation and cleaning room are each equipped with a logistics door connecting to the outside. A protective corridor surrounds the negative pressure protective area on three sides. The first personnel protective area has a personnel entrance / exit door connecting to the outside and is connected to the protective corridor. The BSL-3 core experimental area and the ABSL-3 core experimental area are connected to the second personnel protective area, which in turn is connected to the protective corridor.

[0009] Furthermore, the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory provided by this invention may also have the following characteristics: the minimum wind pressure of the BSL-3 core experimental area is -40 Pa, which is lower than the wind pressure of the second personnel protective area; the minimum wind pressure of the ABSL-3 core experimental area is -80 Pa, which is lower than the wind pressure of the second personnel protective area; the maximum wind pressure of the protective corridor is +30 Pa; the wind pressure of the buffer room is -55 Pa; the wind pressure of the animal quarantine room, the temporary storage room, and the animal carcass storage room is -70 Pa; and the wind pressure of the first preparation and cleaning room, the animal anteroom, and the second preparation and cleaning room is +10 Pa.

[0010] Furthermore, the laboratory layout structure of the secondary protective barrier of the biosafety level 3 laboratory provided by the present invention may also have the following features: wherein, the BSL-3 core experimental area includes a cell culture chamber and a strain preservation chamber; the ABSL-3 core experimental area includes an animal laboratory and a dissection chamber.

[0011] Furthermore, the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory provided by this invention may also have the following features: wherein, the first personnel protection area includes: a first male changing room, a second male changing room, a male shower room, a first female changing room, a second female changing room, and a female shower room; the first male changing room is provided with a personnel entrance and exit for male personnel, a protective door is provided between the first male changing room and the second male changing room, and protective doors are respectively provided between the second male changing room and the male shower room and the protective corridor; the first female changing room is provided with a personnel entrance and exit for female personnel, a protective door is provided between the first female changing room and the second female changing room, and protective doors are respectively provided between the second female changing room and the female shower room and the protective corridor.

[0012] Furthermore, the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory provided by the present invention may also have the following features: the wind pressure value of the first male changing room and the first female changing room is +10Pa; the wind pressure value of the male shower room and the female shower room is +15Pa; and the wind pressure value of the second male changing room and the second female changing room is +20Pa.

[0013] Furthermore, the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory provided by this invention may also have the following features: wherein the second personnel protection area includes: a first protective clothing changing room, a first air shower, a second protective clothing changing room, and a second air shower; the first air shower is provided with protective doors between itself and the BSL-3 core experimental area and the first protective clothing changing room, and a protective door is provided between the first protective clothing changing room and the protective corridor; the second air shower is provided with protective doors between itself and the BSL-3 core experimental area and the second protective clothing changing room, and a protective door is provided between the second protective clothing changing room and the protective corridor.

[0014] Furthermore, the laboratory layout structure of the secondary protective barrier of the level 3 biosafety laboratory provided by the present invention may also have the following features: wherein the wind pressure value of the first protective clothing changing room and the second protective clothing changing room is -15Pa; and the wind pressure value of the first air shower room and the second air shower room is -25Pa.

[0015] Furthermore, the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory provided by the present invention may also have the following feature: all adjacent doors are interlocked.

[0016] Furthermore, the laboratory layout structure of the secondary protective barrier for a level 3 biosafety laboratory provided by this invention may also have the following feature: Personnel entry and exit procedures in this laboratory layout structure are separated by gender, as detailed below:

[0017] The entry procedure for personnel in the BSL-3 biosafety level 3 microbial laboratory is as follows: they pass through the first changing room, the second changing room, the protective corridor, the first protective clothing changing room, and the first air shower in sequence, and then directly enter the BSL-3 core experimental area.

[0018] The entry procedure for personnel in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: they pass through the first changing room, the second changing room, the protective corridor, the second protective clothing changing room, and the second air shower in sequence, and then directly enter the ABSL-3 core experimental area.

[0019] The exit procedure for personnel in a BSL-3 microbial biosafety laboratory or an ABSL-3 animal biosafety laboratory is as follows: passing through the air shower, protective clothing changing room, protective corridor, second changing room, shower room, and first changing room in sequence.

[0020] Furthermore, the laboratory layout structure of the secondary protective barrier for a level 3 biosafety laboratory provided by this invention may also have the following feature: the material flow in and out of this laboratory layout structure is as follows:

[0021] In the BSL-3 biosafety level 3 microbial laboratory, the procedure for the entry of items and samples is as follows: external items and samples first enter the first preparation and cleaning room, and then are disinfected in the first hydrogen peroxide transfer chamber before entering the BSL-3 core experimental area.

[0022] The waste outflow process in the BSL-3 biosafety level 3 laboratory is as follows: waste from the core experimental area of ​​BSL-3 first enters a double-door autoclave for autoclaving and sterilization, then passes through the first preparation and cleaning room, and is finally transported out.

[0023] In the ABSL-3 Level 3 Animal Biosafety Laboratory, the procedure for items entering the laboratory is as follows: external items first enter the second preparation and cleaning room, then are disinfected in the second hydrogen peroxide transfer chamber, and then enter the ABSL-3 core experimental area.

[0024] Animal entry process in the ABSL-3 Level 3 Animal Biosafety Laboratory: Animals first enter the animal pre-room, then undergo dust removal and sterilization at the animal safety receiving station, then enter the animal quarantine room for quarantine, and finally enter the ABSL-3 core experimental area.

[0025] In the ABSL-3 Level 3 Animal Biosafety Laboratory, the feed bedding material enters the following process: external feed bedding material first enters the second preparation and cleaning room, then undergoes high-pressure sterilization in a double-door autoclave, and finally enters the ABSL-3 core experimental area.

[0026] The process for removing animal carcasses and scrapped equipment in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: After experiments in the ABSL-3 core experimental area, the animal carcasses and scrapped equipment first enter the animal remains storage room, then undergo high-pressure sterilization in a step-by-step double-door autoclave, and then pass through the preparation and cleaning room before finally being transported out.

[0027] The beneficial effects of this invention are:

[0028] 1) The laboratory layout structure of the present invention conforms to the secondary protective barrier technology of a level 3 biosafety laboratory. The reasonable setting of each functional room in the negative pressure protection zone and the positive pressure auxiliary protection zone can better prevent the escape and leakage of harmful biological agents inside the laboratory, maintain the internal cleanliness, and improve the overall biosafety of the high-level biosafety laboratory.

[0029] 2) The air pressure values ​​of the ventilation systems in each room of the Level 3 biosafety laboratory secondary protective barrier of the present invention are set scientifically and reasonably, and are set in a gradient. The airflow direction inside the entire laboratory layout structure is reasonable, safe and reliable.

[0030] 3) The laboratory layout structure of the three-level biosafety laboratory with secondary protective barrier of the present invention includes a BSL-3 level three microbial biosafety laboratory and an ABSL-3 level three animal biosafety laboratory. Each biosafety laboratory area is further divided into a negative pressure core protection area and a positive pressure auxiliary protection area, which can be used to conduct microbial biosafety experiments and animal biosafety experiments, with comprehensive experimental functions.

[0031] 4) In the laboratory layout structure of the secondary protective barrier of the three-level biosafety laboratory of the present invention, the flow of personnel, materials (samples), animals and waste is clear and smooth, and the flow is scientific and reasonable, which will not cause chaos, thus improving cleanliness and safety. Attached Figure Description

[0032] Figure 1 This is a basic schematic diagram of the laboratory layout structure of the secondary protective barrier of a level 3 biosafety laboratory in an embodiment of the present invention;

[0033] Figure 2 This is a detailed schematic diagram of the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory in this embodiment of the invention;

[0034] Figure 3 This is a schematic diagram of the personnel entry and exit process in the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory in this embodiment of the invention.

[0035] Figure 4 This is a schematic diagram of the logistics entry and exit process of the BSL-3 core experimental area in this embodiment of the invention;

[0036] Figure 5 This is a schematic diagram of the logistics entry and exit process of the ABSL-3 core experimental area in this embodiment of the invention.

[0037] In the diagram: 100 - First Personnel Protective Area; 200 - Second Personnel Protective Area; 1 - Male First Changing Room; 2 - Male Second Changing Room; 3 - Male Shower Room; 4 - Female First Changing Room; 5 - Female Second Changing Room; 6 - Female Shower Room; 7 - Protective Corridor; 8 - First Protective Suit Changing Room; 9 - First Air Shower Room; 10 - BSL-3 Core Experimental Area; 11 - First Hydrogen Peroxide Transfer Chamber; 12 - Double-Door Autoclave; 13 - First Preparation and Cleaning Room; 14 - Second Protective Suit Changing Room; 15 - Second Air Shower Room; 16 - ABSL-3 Core Experimental Area; 17 - Buffer Room; 18 - Animal Quarantine Room; 19 - Temporary Storage Room; 20 - Animal Carcass Storage Room; 22 - Second Hydrogen Peroxide Transfer Chamber; 23 - Walking Double-Door Autoclave; 24 - Animal Antechamber; 25 - Second Preparation and Cleaning Room; 26-48 - Doors. Detailed Implementation

[0038] To make the technical means, creative features, objectives and effects of this invention easier to understand, the following embodiments, in conjunction with the accompanying drawings, will specifically illustrate the technical solution of this invention.

[0039] <Example 1>

[0040] See Figure 1 This embodiment provides a laboratory layout structure for a secondary protective barrier in a biosafety level 3 laboratory, which includes a negative pressure protection zone and a positive pressure auxiliary protection zone. The negative pressure protection zone includes a BSL-3 core experimental area 10, an ABSL-3 core experimental area 16, a buffer room 17, an animal quarantine room 18, a temporary storage room for supplies 19, an animal carcass storage room 20, and a second personnel protection zone 200. The positive pressure auxiliary protection zone includes an animal anteroom 24, a first preparation and cleaning room 13, a second preparation and cleaning room 25, a protective corridor 7, and a first personnel protection zone 100.

[0041] The BSL-3 core experimental area 10 and the ABSL-3 core experimental area 16 are located in the central position of the overall laboratory layout. The BSL-3 core experimental area 10 includes a cell culture chamber and a culture preservation chamber for conducting microbial experiments. The ABSL-3 core experimental area 16 includes an animal laboratory and a dissection room for conducting animal experiments.

[0042] A buffer room 17 connects the BSL-3 core experimental area 10 and the ABSL-3 core experimental area 16. A door 38 between the buffer room 17 and the BSL-3 core experimental area 10 faces the BSL-3 core experimental area 10. A door 39 between the buffer room 17 and the ABSL-3 core experimental area 16 faces the buffer room 17. Doors 38 and 39 are staggered.

[0043] The protective corridor 7 surrounds the negative pressure protection zone on three sides. The first pre-washing room 13, the animal anteroom 24, and the second pre-washing room 25 surround the other side of the negative pressure protection zone. The first pre-washing room 13 is equipped with a logistics door 45 connecting to the outside; this logistics door 45 is a double-leaf door. The animal anteroom 24 is equipped with a door 46 connecting to the outside. The second pre-washing room 25 is equipped with doors 47 and 48 connecting to the outside.

[0044] A first hydrogen peroxide transfer chamber 11 and a double-door autoclave 12 are respectively installed between the BSL-3 core experimental area 10 and the first preparation cleaning room 13. The first hydrogen peroxide transfer chamber 11 is used for purification, dust removal, and disinfection. The first hydrogen peroxide transfer chamber 11 is unidirectional, with the transfer direction from the first preparation cleaning room 13 to the BSL-3 core experimental area 10. The double-door autoclave 12 is used for autoclaving and sterilization. The double-door autoclave 12 is unidirectional, with the transfer direction from the BSL-3 core experimental area 10 to the first preparation cleaning room 13.

[0045] The ABSL-3 core experimental area 16 is connected to the animal quarantine room 18, the temporary storage room 19, and the animal carcass storage room 20. Door 42 between the ABSL-3 core experimental area 16 and the animal quarantine room 18 faces the animal quarantine room 18; door 43 between the ABSL-3 core experimental area 16 and the temporary storage room 19 faces the temporary storage room 19; and door 44 between the ABSL-3 core experimental area 16 and the animal carcass storage room 20 faces the animal carcass storage room 20. An animal safety receiving station 21 is provided between the animal quarantine room 18 and the animal antechamber 24, leading to the animal quarantine room 18. A second hydrogen peroxide transfer chamber 22 is provided between the temporary storage room 19 and the second preparation and cleaning room 25, leading to the temporary storage room 19. The function of the second hydrogen peroxide transfer chamber 22 is purification, dust removal, and disinfection. The second hydrogen peroxide transfer chamber 22 is unidirectional, with the transfer direction from the second preparation and cleaning room 25 to the temporary storage room 19. The animal carcass storage room 20 and the second preparation and cleaning room 25 are equipped with a step-by-step double-door high-pressure sterilizer 23. The function of the step-by-step double-door high-pressure sterilizer 23 is high-pressure sterilization and disinfection. The step-by-step double-door high-pressure sterilizer 23 is bidirectional. The direction of the outgoing animal carcass and scrapped instruments and equipment is from the animal carcass storage room 20 to the second preparation and cleaning room 25, and the direction of the incoming external feed and bedding is from the second preparation and cleaning room 25 to the animal carcass storage room 20.

[0046] See Figure 1 The first personnel protection zone 100 is equipped with an entrance / exit connecting to the outside world, and it is connected to the protective corridor 7. (See also...) Figure 2 The first personnel protection zone 100 is separated for men and women, including: Men's First Changing Room 1, Men's Second Changing Room 2, Men's Shower Room 3; Women's First Changing Room 4, Women's Second Changing Room 5, and Women's Shower Room 6. Men's First Changing Room 1 is equipped with a main entrance 26 for male personnel. A protective door 27 connects Men's First Changing Room 1 and Men's Second Changing Room 2; a protective door 28 connects Men's Second Changing Room 2 and Men's Shower Room 3; and a protective door 29 connects Men's Second Changing Room 2 and the protective corridor 7. Women's First Changing Room 4 is equipped with a main entrance 30 for female personnel. A protective door 31 connects Women's First Changing Room 4 and Women's Second Changing Room 5; a protective door 32 connects Women's Second Changing Room 5 and Women's Shower Room 6; and a protective door 33 connects Women's Second Changing Room 5 and the protective corridor 7. The protective door 29 between the second male changing room 2 and the protective corridor 7, and the protective door 33 between the second female changing room 5 and the protective corridor 7, both face the direction of the protective corridor 7.

[0047] See Figure 1 BSL-3 core experimental area 10 and ABSL-3 core experimental area 16 are respectively connected to the second personnel protection area 200, which in turn is connected to the protective corridor 7. (See reference...) Figure 2 The second personnel protection zone 200 includes: a first protective clothing changing room 8, a first air shower 9, a second protective clothing changing room 14, and a second air shower 15. A protective door 37 is installed between the first air shower 9 and the BSL-3 core experimental area 10; a protective door 36 is installed between the first air shower 9 and the first protective clothing changing room 8; and a protective door 34 is installed between the first protective clothing changing room 8 and the protective corridor 7. Protective doors 36 and 37 are staggered. Protective door 34 faces the protective corridor 7 and is staggered from protective door 36. A protective door 40 is installed between the second air shower 15 and the BSL-3 core experimental area 16; a protective door 41 is installed between the second air shower 15 and the second protective clothing changing room 14; and a protective door 35 is installed between the second protective clothing changing room 14 and the protective corridor 7. Protective doors 40 and 41 are staggered. Protective door 35 faces the protective corridor 7 and is staggered from protective door 41.

[0048] In the laboratory layout structure of the secondary protective barrier of the Level 3 biosafety laboratory in this embodiment of the invention, all adjacent doors are interlocked and cannot be opened simultaneously.

[0049] The laboratory layout structure of the biosafety level 3 laboratory secondary protection barrier of this invention embodiment simultaneously possesses the secondary protection barrier of both BSL-3 microbial biosafety laboratory and ABSL-3 animal biosafety laboratory:

[0050] 1) The secondary protective barrier of a BSL-3 level 3 microbial biosafety laboratory consists of the following negative pressure protection zone and positive pressure auxiliary protection zone: The negative pressure core protection zone includes: BSL-3 core experimental area 10, air shower rooms (9, 15), protective clothing changing rooms (8, 14), first hydrogen peroxide transfer chamber 11, and double-door autoclave 12. The positive pressure auxiliary protection zone includes: protective corridor 7, second changing room (2, 6), first changing room (1, 4), shower room (3, 5), and first preparation cleaning room 13.

[0051] 2) The secondary protective barrier of the ABSL-3 Level 3 animal biosafety laboratory consists of the following negative pressure protection zone and positive pressure auxiliary protection zone: The negative pressure protection zone includes: the ABSL-3 core experimental area, air shower rooms (9, 15), protective clothing changing rooms (8, 14), animal quarantine room 18, temporary storage room for supplies 19, animal carcass storage room 20, animal safety receiving station 21, second hydrogen peroxide transfer chamber 22, and walking-type double-door autoclave 23. The positive pressure auxiliary protection zone includes: protective corridor 7, second changing room (2, 6), first changing room (1, 4), washroom (3, 5), second preparation and cleaning room 25, and animal anteroom 24.

[0052] In the laboratory layout structure of this invention, the personnel entry and exit process is separated by gender. Figure 3 This is a schematic diagram of the personnel entry and exit process in the laboratory layout structure of the secondary protective barrier of a Level 3 biosafety laboratory according to an embodiment of the present invention. Solid black arrows indicate personnel entry, and hollow white arrows indicate personnel exit.

[0053] See Figure 3 The entry procedure for personnel in the BSL-3 biosafety level 3 microbial laboratory is as follows: pass through the first changing room, the second changing room, the protective corridor 7, the protective clothing changing room, and the air shower room in sequence, and then directly enter the BSL-3 core experimental area 10.

[0054] See Figure 3 The entry procedure for personnel in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: pass through the first changing room, the second changing room, the protective corridor 7, the protective clothing changing room, and the air shower room in sequence, and then directly enter the ABSL-3 core experimental area 16.

[0055] See Figure 3The procedure for personnel exiting a BSL-3 level 3 microbial biosafety laboratory or an ABSL-3 level 3 animal biosafety laboratory is as follows: from the corresponding core experimental area, personnel pass through the air shower, protective clothing changing room, protective corridor, second changing room, shower room, and first changing room in sequence.

[0056] In the laboratory layout structure of this invention, the flow of materials in and out is divided into "materials (samples) flow, animal flow, and waste flow". Figure 4 This is a schematic diagram of the logistics entry and exit process of the BSL-3 core experimental area in this embodiment of the invention. Figure 5 This is a schematic diagram of the logistics entry and exit process of the ABSL-3 core experimental area in this embodiment of the invention. Solid black arrows in the diagram represent logistics entry, and hollow white arrows represent logistics exit.

[0057] See Figure 4 In the BSL-3 biosafety level 3 laboratory, the process for items and samples to enter is as follows: as shown by the solid black arrow in the figure, external items and samples first enter the first preparation and cleaning room 13, and then are disinfected in the first hydrogen peroxide transfer chamber 11 before entering the BSL-3 core experimental area 10.

[0058] See Figure 4 The waste outflow process in the BSL-3 biosafety level 3 laboratory is as follows: as shown by the hollow white arrow in the figure, the waste in the BSL-3 core experimental area 10 first enters the double-door autoclave 12 for autoclaving and sterilization, then passes through the first preparation and cleaning room 13, and is finally transported out.

[0059] See Figure 5 In the ABSL-3 Level 3 Animal Biosafety Laboratory, the procedure for items entering the laboratory is as shown by arrow group b in the figure. External items first enter the second preparation and cleaning room 25, then are disinfected in the second hydrogen peroxide transfer chamber 22, and then enter the ABSL-3 core experimental area 16.

[0060] See Figure 5 The animal entry process in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: as shown by arrow group a in the figure, the animal first enters the animal pre-room 24, then passes through the animal safety receiving station 21 for dust removal and sterilization, enters the animal quarantine room 18 for quarantine, and finally enters the ABSL-3 core experimental area 16.

[0061] See Figure 5 In the ABSL-3 Level 3 Animal Biosafety Laboratory, the feed bedding material enters the following process: as shown by arrow group c in the figure, the feed bedding material first enters the second preparation and cleaning room 25, then undergoes high-pressure sterilization in the step-type double-door autoclave 23, and finally enters the ABSL-3 core experimental area.

[0062] See Figure 5The process for removing animal carcasses and scrapped instruments and equipment in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: As shown by arrow group d in the figure, after the experiment in the ABSL-3 core experimental area 16, the animal carcasses and scrapped instruments and equipment first enter the animal remains storage room 20, then undergo high-pressure sterilization in the step-type double-door high-pressure sterilizer 23, and then pass through the preparation and cleaning room before finally being transported out.

[0063] <Example 2>

[0064] This embodiment further optimizes the wind pressure values ​​of the ventilation systems in each functional room based on the laboratory layout structure of the Level 3 biosafety laboratory with a secondary protective barrier in Embodiment 1, so as to form a scientific and reasonable airflow direction inside the laboratory layout structure, thereby improving its indoor cleanliness and biosafety.

[0065] In the positive pressure auxiliary protection zone, the wind pressure values ​​of male first changing room 1 and female first changing room 4 are both +10 Pa. The wind pressure values ​​of male shower room 3 and female shower room 6 are both +15 Pa. The wind pressure values ​​of male second changing room 2 and female second changing room 5 are both +20 Pa. The wind pressure value of the protective corridor 7 is higher than that of male second changing room 2 and female second changing room 5. Therefore, the airflow can only flow from the positive pressure protective corridor 7 towards the first changing room. As a result, outdoor dust particles, microorganisms, etc., cannot flow into the positive pressure auxiliary protection zone, thus ensuring the cleanliness of all functional rooms in the biosafety level 3 laboratory. The highest wind pressure value of the protective corridor 7 is +30 Pa, and the protective corridor 7 is surrounded on three sides by the isolation negative pressure protection zone. The protective corridor 7 is the area with the highest wind pressure value in the entire laboratory layout structure. Therefore, the airflow flows from the positive pressure protective corridor to the negative pressure protection zone, effectively helping to prevent harmful biological agents inside the core experimental area from escaping and leaking out, thus contaminating the external environment. The air pressure values ​​of the first preparation and cleaning room 13, the animal anteroom 24, and the second preparation and cleaning room 25 are all +10Pa.

[0066] In the negative pressure protection zone, the air pressure values ​​of the first protective clothing changing room 8 and the second protective clothing changing room 14 are both -15 Pa. The air pressure values ​​of the first air shower room 9 and the second air shower room 15 are both -25 Pa. The lowest air pressure value of the BSL-3 core experimental area 10 is -40 Pa, which is lower than the air pressure value of the first air shower room 9. Airflow can only flow into the BSL-3 core experimental area, effectively preventing harmful biological agents inside the BSL-3 core experimental area from escaping and leaking out and polluting the external environment. The air pressure value of the ABSL-3 core experimental area 16 is lower than the air pressure value of the second air shower room 15. Airflow can only flow into the ABSL-3 core experimental area, effectively preventing harmful biological agents inside the ABSL-3 core experimental area from escaping and leaking out and polluting the external environment. The air pressure value of the ABSL-3 core experimental area is lower than that of the BSL-3 core experimental area, with the lowest air pressure value of the ABSL-3 core experimental area 16 being -80 Pa. The air pressure value of the buffer room 17 is -55 Pa. The wind pressure values ​​of the animal quarantine room 18, the temporary storage room 19, and the animal carcass storage room 20 are all -70Pa.

[0067] In this embodiment, the laboratory layout structure of the Level 3 biosafety laboratory with secondary protective barriers ensures that harmful biological agents cannot leak out of the laboratory by setting reasonable pressure gradients for each functional room, and that the overall cleanliness of the laboratory meets the national standard cleanliness requirements.

[0068] The above embodiments are merely preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. For those skilled in the art, the present invention can have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention are included within the scope of protection of the present invention.

Claims

1. A laboratory layout structure for a secondary protective barrier in a biosafety level 3 laboratory, characterized in that, include: Negative pressure protection zone and positive pressure auxiliary protection zone, The negative pressure protection area includes the BSL-3 core experimental area, the ABSL-3 core experimental area, a buffer room, an animal quarantine room, a temporary storage room for goods, an animal carcass storage room, and a second personnel protection area. The positive pressure auxiliary protection area includes an animal anteroom, a first preparation and cleaning room, a second preparation and cleaning room, a protective corridor, and a first personnel protection area; The buffer room connects the BSL-3 core experimental area and the ABSL-3 core experimental area. The BSL-3 core experimental area is connected to the first preparation cleaning room by a double-door high-pressure sterilizer leading to the first preparation cleaning room and a first hydrogen peroxide transfer chamber leading to the BSL-3 core experimental area. The ABSL-3 core experimental area is connected to the animal quarantine room, the temporary storage room for goods, and the animal carcass storage room, respectively. An animal safety receiving station leading to the animal quarantine room is set up between the animal quarantine room and the animal anteroom. A second hydrogen peroxide transfer chamber leading to the temporary storage room is provided between the temporary storage room and the second preparation cleaning room. The animal carcass storage room and the second preparation and cleaning room are equipped with a double-door high-pressure sterilizer. The first preparation and cleaning room, the animal antechamber, and the second preparation and cleaning room are each equipped with a logistics door that connects to the outside. The protective corridor surrounds the negative pressure protection zone on three sides; The first personnel protection zone is equipped with a personnel entrance and exit that connects to the outside world, and the first personnel protection zone is connected to the protective corridor; The BSL-3 core experimental area and the ABSL-3 core experimental area are respectively connected to the second personnel protection area, and the second personnel protection area is connected to the protective corridor.

2. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, The minimum wind pressure in the BSL-3 core experimental area is -40Pa, which is lower than the wind pressure in the second personnel protection area. The lowest wind pressure in the ABSL-3 core experimental area is -80Pa, which is lower than the wind pressure in the second personnel protection area. The maximum wind pressure in the protective corridor is +30Pa; The wind pressure value of the buffer room is -55Pa; The wind pressure value of the animal quarantine room, the temporary storage room for goods, and the animal carcass storage room is -70Pa; The air pressure values ​​of the first preparation cleaning room, the animal antechamber, and the second preparation cleaning room are all +10Pa.

3. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, The BSL-3 core experimental area includes a cell culture chamber and a bacterial culture preservation chamber. The ABSL-3 core experimental area includes an animal laboratory and a dissection room.

4. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, The first personnel protection zone includes: male first changing room, male second changing room, male shower room, female first changing room, female second changing room, and female shower room; The first male changing room is equipped with an entrance and exit for male personnel. A protective door is installed between the first male changing room and the second male changing room. Protective doors are also installed between the second male changing room and the male shower room and the protective corridor. The first female changing room is equipped with an entrance and exit for female personnel. A protective door is installed between the first female changing room and the second female changing room. Protective doors are also installed between the second female changing room and the female bathroom and the protective corridor.

5. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 4, characterized in that: in, The wind pressure value of the first male changing room and the first female changing room is +10Pa; The wind pressure value of the men's bathroom and the women's bathroom is +15Pa; The wind pressure value of the second men's changing room and the second women's changing room is +20Pa.

6. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, The second personnel protection area includes: a first protective clothing changing room, a first air shower, a second protective clothing changing room, and a second air shower; The first air shower room is connected to the BSL-3 core experimental area and the first protective clothing changing room by protective doors, and the first protective clothing changing room is connected to the protective corridor by protective doors. The second air shower is connected to the ABSL-3 core experimental area and the second protective clothing changing room by protective doors, and the second protective clothing changing room is connected to the protective corridor by a protective door.

7. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 6, characterized in that: in, The wind pressure value of the first protective clothing changing room and the second protective clothing changing room is -15Pa; The air pressure value of the first air shower and the second air shower is -25Pa.

8. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, All adjacent doors are interlocked.

9. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, In the layout of this laboratory, the personnel entry and exit process is as follows: the first personnel protection zone is separated by gender, while the second personnel protection zone, BSL-3 and ABSL-3, are independent and not separated by gender, as detailed below: The entry procedure for personnel in the BSL-3 biosafety level 3 microbial laboratory is as follows: they pass through the first changing room, the second changing room, the protective corridor, the first protective clothing changing room, and the first air shower in sequence, and then directly enter the BSL-3 core experimental area. The entry procedure for personnel in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: they pass through the first changing room, the second changing room, the protective corridor, the second protective clothing changing room, and the second air shower in sequence, and then directly enter the ABSL-3 core experimental area. The exit procedure for personnel in a BSL-3 microbial biosafety laboratory or an ABSL-3 animal biosafety laboratory is as follows: passing through the air shower, protective clothing changing room, protective corridor, second changing room, shower room, and first changing room in sequence.

10. The laboratory layout structure of the secondary protective barrier of a biosafety level 3 laboratory as described in claim 1, characterized in that: in, The logistics flow in and out of this laboratory layout is as follows: In the BSL-3 biosafety level 3 microbial laboratory, the procedure for the entry of items and samples is as follows: external items and samples first enter the first preparation and cleaning room, and then are disinfected in the first hydrogen peroxide transfer chamber before entering the BSL-3 core experimental area. The waste outflow process in the BSL-3 biosafety level 3 laboratory is as follows: waste from the core experimental area of ​​BSL-3 first enters a double-door autoclave for autoclaving and sterilization, then passes through the first preparation and cleaning room, and is finally transported out. In the ABSL-3 Level 3 Animal Biosafety Laboratory, the procedure for items entering the laboratory is as follows: external items first enter the second preparation and cleaning room, then are disinfected in the second hydrogen peroxide transfer chamber, and then enter the ABSL-3 core experimental area. Animal entry process in the ABSL-3 Level 3 Animal Biosafety Laboratory: Animals first enter the animal pre-room, then undergo dust removal and sterilization at the animal safety receiving station, then enter the animal quarantine room for quarantine, and finally enter the ABSL-3 core experimental area. In the ABSL-3 Level 3 Animal Biosafety Laboratory, the feed bedding material enters the following process: external feed bedding material first enters the second preparation and cleaning room, then undergoes high-pressure sterilization in a double-door autoclave, and finally enters the ABSL-3 core experimental area. The process for removing animal carcasses and scrapped equipment in the ABSL-3 Level 3 Animal Biosafety Laboratory is as follows: After experiments in the ABSL-3 core experimental area, the animal carcasses and scrapped equipment first enter the animal remains storage room, then undergo high-pressure sterilization in a step-by-step double-door autoclave, and then pass through the preparation and cleaning room before finally being transported out.

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