Preparation and Application of a Bryophyte Gametophyte Coating Agent

Abstract

The present invention provides a moss gametophyte coating agent, comprising: 80-140 parts by weight of a composite additive, 40-60 parts of a film-forming agent, 10-20 parts of a medicament, 8-20 parts of an antifreeze agent, 200-300 parts of a nutrient matrix, 0.01-1 part of a growth regulator, 20-50 parts of an emulsifier, 10-30 parts of a dispersant, 10-30 parts of an anti-freezing agent, 10-20 parts of an anti-foaming agent, and 600-800 parts of deionized water; wherein, the composite additive is modified montmorillonite, the medicament is a combination of chitosan and garlic extract, and the antifreeze agent is an algal extract. The moss gametophyte coating agent provided by the present invention is used for coating the moss gametophyte, significantly improving the survival rate, germination rate of the moss gametophyte and the stress tolerance to habitats such as high cold, drought and barrenness, and effectively breaking through the technical bottleneck of the field breeding of the moss gametophyte.

Description

Technical Field

[0001] The invention relates to the field of coating agents and preparation methods thereof, and in particular to the preparation and application of a moss gametophyte coating agent. Background Art

[0002] Due to the natural environment, ore mining, urbanization and other reasons, there are a lot of bare rocks, which not only hinder the urban landscape, but also easily cause soil erosion and restrict regional development. Existing technologies use hanging nets, soil spraying, planting hanging plants and climbing plants to green rocks, but most greening is difficult to last long, or the greening effect is not ideal, and some slopes are even affected by plant roots, losing stability and inducing geological disasters.

[0003] Moss crusts play an important role in ecosystem services such as water conservation, soil conservation, and biodiversity maintenance. They can solve the greening problem of rock slopes and are considered important greening plants. Outdoor cultivation of mosses mainly relies on gametophytes for reproduction. Under unfavorable habitat conditions, the gametophytes of mosses will reduce or stop growing and exist in a dormant state to survive the adverse environmental period. Once environmental conditions become suitable, they can quickly resume normal physiological activities and begin to grow to continue their life history. Moss gametophyte coating breeding technology is a new seed technology formed by drawing on seed coating technology. Its main purpose is to improve the survival rate and germination rate of moss gametophyte stem segments and their resistance to high-altitude, drought, and barren habitats.

[0004] At present, my country's seed coating technology is mainly used in field crops, such as wheat, corn, cotton, soybean, rice and other food and economic crops. There are few patent reports related to moss gametophyte coating. Therefore, it is of great significance to improve the structural formula of existing coating agents so that the coating agents can meet the requirements for the growth of moss gametophytes on the bare rock surface. Summary of the invention

[0005] An object of the present invention is to solve at least one of the above-mentioned problems and / or disadvantages and to provide at least the advantages described hereinafter.

[0006] In order to achieve these purposes and other advantages according to the present invention, the present invention provides a moss gametophyte coating agent, comprising: in parts by weight, 80-140 parts of composite additives, 40-60 parts of film-forming agents, 10-20 parts of medicaments, 8-20 parts of antifreeze agents, 200-300 parts of nutrient matrix, 0.01-1 part of growth regulators, 20-50 parts of emulsifiers, 10-30 parts of dispersants, 10-30 parts of antifreeze agents, 10-20 parts of defoaming agents, and 600-800 parts of deionized water; wherein the composite additive is modified montmorillonite; the medicament is a combination of chitosan and garlic extract, and the mass ratio of chitosan to garlic extract is 1-4:2-4; the antifreeze agent is a seaweed extract.

[0007] Preferably, the film-forming agent is one or a combination of soluble starch, polyvinyl alcohol, polyethylene glycol, konjac glucomannan, xanthan gum, gum arabic, hydroxypropyl methylcellulose.

[0008] Preferably, the growth regulator is one or a combination of gibberellin, chlormequat chloride, brassinolide, uniconazole, and diethyl aminoethyl hexanoate.

[0009] Preferably, the nutrient matrix is a combination of organic humus and MS nutrient solution containing only inorganic components, and the mass ratio of the humus to the MS nutrient solution is 1-4:1-4.

[0010] Preferably, the dispersant is any one or a combination of sodium dodecyl sulfonate, sodium dodecyl sulfate, and sodium lignosulfonate; the antifreeze is any one or a combination of ethanol, ethylene glycol, and propylene glycol; the defoamer is emulsified silicone oil or polyether defoamer.

[0011] Preferably, the preparation method of the modified montmorillonite powder is as follows:

[0012] S1. Weigh 20-30 parts of montmorillonite in 1000-1500 parts of deionized water, ultrasonically exfoliate at 40-60 kHz for 1-3 h and then centrifuge; take the supernatant after centrifugation, add 10-15 parts of tannic acid to the supernatant, and under ultrasonic irradiation at 40-60 kHz, stir and react for 12-24 h to obtain a mixed solution;

[0013] S2. Weigh 0.01-1 part of potassium persulfate into the mixed solution after stirring and reacting in S1, heat to 60-80 °C, stir for 20-40 min, then add 12-20 parts of acrylamide and 10-20 parts of sodium styrene sulfonate, keep warm and continue to stir and react for 6-12 h, filter, wash, dry, grind, and sieve to obtain modified montmorillonite.

[0014] The present invention also provides a preparation method of a moss gametophyte coating agent, including the following steps: Add an emulsifier, a dispersant, an antifreeze, and a defoamer into deionized water, heat to 50-70 °C, stir and dissolve evenly, then add modified montmorillonite and a film-forming agent, continue to stir and mix evenly, wait until it cools to room temperature, add a medicament, an antifreeze agent, a nutrient matrix, and a growth regulator, and use a high-speed shear emulsifier to mix evenly to obtain a viscous mixture.

[0015] An application of a moss gametophyte coating agent, including a method for treating moss gametes with the moss gametophyte coating agent.

[0016] Preferably, the method for treating moss gametophytes with the moss gametophyte coating agent includes: selecting healthy and mature moss gametophytes, cutting them into stem segments 1-3 cm long, soaking them in alcohol with a concentration of 70-80% for 10-20 s, rinsing them clean, then soaking them in a sodium hypochlorite solution with a concentration of 1-2 wt% for 10-30 s, rinsing them clean with sterile water, then immersing all the moss stem segments in the prepared coating agent for 2-4 s, lifting them up, and placing them in a cool place to dry.

[0017] Preferably, the moss stem segments are any one of Racomitrium japonicum stem segments, Hypnum plumaeforme stem segments, Climacium dendroides stem segments, Plagiomnium acutum stem segments, Hypnum cupressiforme, Polytrichum commune, Bryum argenteum stem segments.

[0018] Compared with the prior art, the present invention has at least the following beneficial effects:

[0019] First, through the moss gametophyte coating technology, the present invention significantly improves the survival rate, germination rate of moss gametophytes and their stress tolerance to habitats such as high cold, drought and barrenness, and effectively breaks through the technical bottleneck of moss gametophyte breeding in the wild.

[0020] Second, the present invention uses humus and MS nutrient solution to provide nutrients for the growth and germination of moss gametophytes, uses chitosan and garlic extract as antibacterial agents to reduce the incidence rate, uses auxin regulators to improve the germination rate and emergence rate of moss gametophytes under low temperature conditions, uses modified montmorillonite powder as a composite additive. The modified montmorillonite powder has a super water retention effect, can enrich nitrogen, phosphorus, potassium and trace elements. Combined with a film-forming agent, a uniform film is solidified on the stem segments by means of the film-forming agent. After field spraying, the coating agent forms a protective barrier for the gametophytes, swells after absorbing water, will not be dissolved immediately and the active ingredients are gradually released. Along with the germination, sprouting and growth and development of the gametophytes, the water and active ingredients are gradually absorbed by the moss plants, which can not only improve the tolerance of moss stem segment seedlings to various field adversities, but also promote the growth establishment and rapid greening of moss in the wild.

[0021] Other advantages, objectives and features of the present invention will be partially reflected by the following description, and partially will be understood by those skilled in the art through the research and practice of the invention. Detailed Embodiments

[0022] The following describes the specific solutions proposed by the present invention in more detail with several specific embodiments.

[0023] Example 1

[0024] The method for coating moss stem segments with a moss gametophyte coating agent includes:

[0025] S1. Prepare modified montmorillonite, including: Weigh 20 g of sodium-based montmorillonite into 1 L of deionized water, ultrasonically exfoliate it at 40 kHz for 2 h and then centrifuge; Take the supernatant and add it to a reaction kettle, add 15 g of tannic acid, and under ultrasonic irradiation at 40 kHz, stir and react at 400 rpm for 16 h. Then add 0.5 g of potassium persulfate as an initiator to the reaction kettle, heat it to 80 °C, stir for 30 min, add 12 g of acrylamide and 15 g of sodium styrenesulfonate for graft copolymerization, keep warm and continue to stir and react for 6 h, filter, wash, dry, grind, and sieve through a 100-mesh sieve to obtain modified montmorillonite powder;

[0026] S2. Prepare the bryophyte gametophyte coating agent, including: Weigh 40 g of fatty alcohol polyoxyethylene ether, 12 g of sodium dodecyl sulfonate, 15 g of ethylene glycol, and 10 g of dimethyl silicone oil, add them to 660 g of deionized water, heat to 60 °C, stir and dissolve, then add 100 g of modified montmorillonite powder, 20 g of polyvinyl alcohol, 15 g of konjac glucomannan, and 15 g of gum arabic, stir evenly. After cooling to room temperature, add 8 g of chitosan, 8 g of garlic extract, 10 g of seaweed extract, 100 g of MS nutrient solution, 150 g of humus, 20 mg of chlormequat chloride, and 20 mg of brassinolide, and use a high-speed shear emulsifier to mix evenly. The viscous mixture obtained is the bryophyte gametophyte coating agent.

[0027] S3. Coating treatment for bryophyte gametophytes: Select healthy and mature Hypnum plumaeforme gametophytes, cut them into stem segments of 1 - 3 cm, soak them in 75% alcohol for 12 s, rinse them clean, then soak them in a 1 wt% sodium hypochlorite solution for 20 s, rinse them clean with sterile water, then immerse all the bryophyte stem segments in the prepared coating agent for 2 s, lift them up, and place them in a cool place to dry to obtain gametophyte coatings.

[0028] Example 2

[0029] A method for coating bryophyte stem segments with bryophyte gametophyte coatings, including:

[0030] S1. Prepare modified montmorillonite, and its specific preparation method and components are the same as those in Example 1, which will not be elaborated here;

[0031] S2. Prepare the bryophyte gametophyte coating agent, including:

[0032] Weigh 20 g of styrylphenol polyoxyethylene ether, 20 g of fatty alcohol polyoxyethylene ether, 16 g of sodium dodecyl sulfonate, 20 g of ethylene glycol, and 12 g of dimethyl silicone oil, add them to 700 g of deionized water, heat to 55 °C, stir to dissolve, then add 120 g of modified montmorillonite powder, 30 g of polyvinyl alcohol, 20 g of konjac glucomannan, and 10 g of xanthan gum, stir evenly. After cooling to room temperature, add 12 g of chitosan, 6 g of garlic extract, 12 g of seaweed extract, 150 g of MS nutrient solution, 150 g of humus, and 40 mg of gibberellin. Mix evenly with a high-speed shear emulsifier, and the obtained viscous mixture is the moss gametophyte coating agent.

[0033] S3. Method for coating moss gametophytes: Select healthy and mature Hypnum plumaeforme gametophytes, cut them into stem segments of 1 - 3 cm, soak them in 75% alcohol for 12 s, rinse thoroughly, then soak them in a 1 wt% sodium hypochlorite solution for 20 s. After rinsing thoroughly with sterile water, immerse all the moss stem segments in the prepared coating agent for 2 s, then lift them out and place them in a cool place to dry, obtaining the gametophyte coating.

[0034] Example 3

[0035] Method for coating moss stem segments with moss gametophyte coating, including:

[0036] S1. Prepare modified montmorillonite, and its specific preparation method and components are the same as those in Example 1, which will not be elaborated here;

[0037] S2. Configure the moss gametophyte coating agent, including: Weigh 20 g of styrylphenol polyoxyethylene ether, 20 g of fatty alcohol polyoxyethylene ether, 16 g of sodium dodecyl sulfonate, 25 g of ethylene glycol, and 12 g of dimethyl silicone oil, add them to 700 g of deionized water, heat to 55 °C, stir to dissolve, then add 140 g of modified montmorillonite powder, 30 g of polyvinyl alcohol, 20 g of konjac glucomannan, and 10 g of soluble starch, stir evenly. After cooling to room temperature, add 12 g of chitosan, 6 g of garlic extract, 15 g of seaweed extract, 150 g of MS nutrient solution, 100 g of humus, 20 mg of uniconazole, and 20 mg of gibberellin. Mix evenly with a high-speed shear emulsifier, and the obtained viscous mixture is the moss gametophyte coating agent.

[0038] S3. Method for coating moss gametophytes: Select healthy and mature Hypnum plumaeforme gametophytes, cut them into stem segments of 1 - 3 cm, soak them in 75% alcohol for 12 s, rinse thoroughly, then soak them in a 1 wt% sodium hypochlorite solution for 20 s. After rinsing thoroughly with sterile water, immerse all the moss stem segments in the prepared coating agent for 2 s, then lift them out and place them in a cool place to dry, obtaining the gametophyte coating.

[0039] Example 4

[0040] The difference between this embodiment and Embodiment 1 is that: the gametophyte of Hypnum plumaeforme is replaced by the gametophyte of Plagiomnium medium.

[0041] Embodiment 5

[0042] The difference between this embodiment and Embodiment 1 is that: the gametophyte of Hypnum plumaeforme is replaced by the gametophyte of Plagiomnium medium.

[0043] Embodiment 6

[0044] The difference between this embodiment and Embodiment 2 is that: the gametophyte of Hypnum plumaeforme is replaced by the gametophyte of Plagiomnium acutum.

[0045] Embodiment 7

[0046] The difference between this embodiment and Embodiment 2 is that: the gametophyte of Hypnum plumaeforme is replaced by the gametophyte of Polytrichum commune.

[0047] Embodiment 8

[0048] The difference between this embodiment and Embodiment 3 is that: the gametophyte of Hypnum plumaeforme is replaced by the gametophyte of Bryum argenteum.

[0049] Embodiment 9

[0050] The difference between this embodiment and Embodiment 3 is that: the gametophyte of Hypnum plumaeforme is replaced by the gametophyte of Eurhynchium hians.

[0051] Comparative Example 1

[0052] A method for coating moss stem segments with a moss gametophyte coating agent, comprising:

[0053] S1. Prepare the moss gametophyte coating agent, including: weighing 40 g of fatty alcohol polyoxyethylene ether, 12 g of sodium dodecyl sulfonate, 20 g of ethylene glycol, 10 g of dimethyl silicone oil, adding them to 660 g of deionized water, heating to 60 °C, stirring and dissolving, then adding 100 g of commercially available sodium-based montmorillonite powder, 20 g of polyvinyl alcohol, 15 g of konjac glucomannan, and 15 g of gum arabic, stirring evenly, after cooling to room temperature, adding 8 g of chitosan, 8 g of garlic extract, 12 g of seaweed extract, 100 g of MS nutrient solution, 150 g of humus, 20 mg of chlormequat chloride, and 20 mg of brassinolide, and using a high-speed shear emulsifier to mix evenly to obtain a viscous mixture, which is the moss gametophyte coating agent;

[0054] S2. Coating treatment of bryophyte gametophytes: Select healthy and mature Hypnum plumaeforme gametophytes, cut them into stem segments of 1 - 3 cm, soak them in 75% alcohol for 12 s, rinse them thoroughly, then soak them in 1 wt% sodium hypochlorite solution for 20 s. After rinsing them thoroughly with sterile water, immerse all the bryophyte stem segments into the prepared coating agent for 2 s, then lift them out and let them dry in a cool place to obtain coated gametophytes.

[0055] Comparative Example 2

[0056] A method for coating bryophyte stem segments with a bryophyte gametophyte coating agent, comprising:

[0057] S1. Prepare the bryophyte gametophyte coating agent, including: Weigh 40 g of fatty alcohol polyoxyethylene ether, 12 g of sodium dodecyl sulfonate, 20 g of ethylene glycol, 10 g of dimethyl silicone oil, add them to 660 g of deionized water, heat to 60°C, stir and dissolve. Then add 20 g of polyvinyl alcohol, 15 g of konjac glucomannan and 15 g of gum arabic, stir evenly. After cooling to room temperature, add 8 g of chitosan, 8 g of garlic extract, 12 g of seaweed extract, 100 g of MS nutrient solution, 150 g of humus, 20 mg of chlormequat chloride and 20 mg of brassinolide, and use a high-speed shear emulsifier to mix evenly. The viscous mixture obtained is the bryophyte gametophyte coating agent.

[0058] S2. Coating treatment of bryophyte gametophytes: Select healthy and mature Hypnum plumaeforme gametophytes, cut them into stem segments of 1 - 3 cm, soak them in 75% alcohol for 12 s, rinse them thoroughly, then soak them in 1 wt% sodium hypochlorite solution for 20 s. After rinsing them thoroughly with sterile water, immerse all the bryophyte stem segments into the prepared coating agent for 2 s, then lift them out and let them dry in a cool place to obtain coated gametophytes.

[0059] Experimental Example 1

[0060] Determine the water retention ability of the bryophyte gametophyte coating agent: At room temperature, weigh the same weight of the coated bryophyte gametophytes of Examples 1 - 3 and Comparative Examples 1 - 2, denoted as m1. Place each coated bryophyte gametophyte in a petri dish, spray deionized water on the surface of each coated bryophyte gametophyte once, weigh and denote as m2. After standing for 1 h, weigh each coated bryophyte gametophyte coating again and denote as m3; The water retention rate Q of the coating agent = (m3 - m1) / (m2 - m1) × 100%.

[0061] Experimental data:

[0062] Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Water retention rate (Q) 38.3％ 40.6％ 41.3％ 21.7％ 15.4％

[0063] The water retention ability of the coating agent added with modified montmorillonite powder is significantly enhanced. It can be expected that after the bryophyte gametophytes are treated with the coating agent, their tolerance in arid environments is enhanced.

[0064] Experimental Example 2

[0065] Determine the germination rate of coated moss gametophytes under alpine conditions: Sow and attach the prepared coated moss gametophytes and uncoated moss gametophytes on a culture dish in an incubator. The culture dish contains MS medium + 20 g / L of white sugar, and the pH value is 6.0; the temperature is 10°C, the light intensity is 2800 lx, the day length is 16 h, the night length is 8 h, and the relative humidity is 70%; Observe and record the germination number and cumulative germination number of each plant every day. On the 25th day, count the germination rate of each moss gametophyte. Germination rate = number of germinated gametophytes / total sown number × 100.

[0066] Experimental data:

[0067]

[0068] After the moss gametophytes are treated by a coating machine, their germination rate in the alpine environment is significantly better than that of the uncoated ones, and the dosage of modified montmorillonite powder has little effect on the germination of gametophytes.

[0069] Experimental Example 3

[0070] Conduct a greening test on bare rock in the wild with coated moss gametophytes: The average temperature at the test site is about 18°C, and the lowest temperature is higher than 6°C. The area of each test planting site is 1.5 × 2 m. The sowing method is broadcasting, and the sowing rate is 80 g / m 2 , and the specific method of bare rock greening refers to Patent CN113875534B, which will not be elaborated here; Observe and record the growth status of these plants every day and measure the growth rate of each plant by the net growth length method: Select 10 plants in good condition for each type of moss gametophyte and mark them with thin lines of different colors. Record the length from the marked place to the growth tip every 10 days, and conduct statistics after 2 months.

[0071] Experimental data:

[0072]

[0073] Regarding the reproduction of moss gametophytes on the surface of bare rock, the components of the coating machine of the present invention have considered various conditions such as drought resistance, antibacterial, cold resistance, and nutrition. As can be seen from the above Experimental Examples 1 to 3, the coating agent of the present invention not only has an excellent water retention effect, significantly improves the germination rate of moss gametophytes in a low-temperature environment, but also promotes the growth rate of moss gametophytes, enhances the stress tolerance of moss gametophytes to alpine, arid, and barren habitats, breaks through the technical bottleneck of moss gametophyte breeding in the wild, and helps to promote the growth establishment and rapid greening of moss in the wild.

[0074] Finally, it should be noted that the above-described embodiments are only specific embodiments of the present invention, used to illustrate the technical solutions of the present invention, rather than limiting it. The protection scope of the present invention is not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that any person skilled in the art within the technical scope disclosed by the present invention can still modify the technical solutions recorded in the foregoing embodiments, or can easily think of changes, or perform equivalent replacements on some of the technical features; and these modifications, changes or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention, and should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims described.

Claims

1. A moss gametophyte coating agent, characterized in that, Comprising: By weight, 80 - 140 parts of composite additive, 40 - 60 parts of film-forming agent, 10 - 20 parts of medicament, 8 - 20 parts of antifreeze agent, 200 - 300 parts of nutrient matrix, 0.01 - 1 part of growth regulator, 20 - 50 parts of emulsifier, 10 - 30 parts of dispersant, 10 - 30 parts of antifreeze, 10 - 20 parts of defoamer, 600 - 800 parts of deionized water; wherein, the composite additive is modified montmorillonite; the medicament is a combination of chitosan and garlic extract, and the mass ratio of chitosan to garlic extract is 1 - 4:2 - 4; the antifreeze agent is an algal extract; The preparation method of the modified montmorillonite: S1. By weight, weigh 20 - 30 parts of montmorillonite in 1000 - 1500 parts of deionized water, ultrasonically exfoliate at 40 - 60 kHz for 1 - 3 h and then centrifuge; take the supernatant after centrifugation, add 10 - 15 parts of tannic acid to the supernatant, and under ultrasonic irradiation at 40 - 60 kHz, stir and react for 12 - 24 h to obtain a mixed solution; S2. By weight, add 0.01 - 1 part of potassium persulfate to the mixed solution after stirring and reacting in S1, heat to 60 - 80 °C, stir for 20 - 40 min, then add 12 - 20 parts of acrylamide and 10 - 20 parts of sodium styrenesulfonate, keep warm and continue to stir and react for 6 - 12 h, filter, wash, dry, grind, and sieve to obtain modified montmorillonite.

2. The moss gametophyte coating agent according to claim 1, characterized in that, The film-forming agent is one or a combination of soluble starch, polyvinyl alcohol, polyethylene glycol, konjac glucomannan, xanthan gum, gum arabic, hydroxypropyl methylcellulose.

3. The moss gametophyte coating agent according to claim 1, characterized in that, The growth regulator is one or a combination of gibberellin, chlormequat chloride, brassinolide, uniconazole, amine fresh ester.

4. The moss gametophyte coating agent according to claim 1, characterized in that, The nutrient matrix is a combination of organic humus and MS nutrient solution containing only inorganic components, and the mass ratio of humus to MS nutrient solution is 1 - 4:1 - 4.

5. The moss gametophyte coating agent according to claim 1, characterized in that, The dispersant is any one or a combination of sodium dodecyl sulfonate, sodium dodecyl sulfate, sodium lignin sulfonate; the antifreeze is any one or a combination of ethanol, ethylene glycol, glycerol; the defoamer is emulsifying silicone oil or polyether defoamer.

6. A preparation method of the moss gametophyte coating agent according to any one of claims 1 to 5, characterized in that, Including the following steps: Add the emulsifier, dispersant, antifreeze, and defoamer together to deionized water, heat to 50 - 70 °C, stir and dissolve evenly, then add the modified montmorillonite and film-forming agent, continue to stir and mix evenly, wait until it cools to room temperature, add the medicament, antifreeze agent, nutrient matrix, and growth regulator, and use a high-speed shear emulsifier to mix evenly to obtain a viscous mixture.

7. Use of a moss gametophyte coating agent according to any one of claims 1 to 5, characterized in that, Including a method for treating bryophyte gametophytes with the bryophyte gametophyte coating agent.

8. The application of the moss gametophyte coating agent according to claim 7, characterized in that, The method for treating bryophyte gametophytes with the bryophyte gametophyte coating agent includes: Select healthy and mature bryophyte gametophytes, cut them into stem segments of 1 - 3 cm, soak them in alcohol with a concentration of 70 - 80% for 10 - 20 s, rinse them clean, then soak them in a sodium hypochlorite solution with a concentration of 1 - 2 wt% for 10 - 30 s, after rinsing them clean with sterile water, immerse all the bryophyte stem segments in the prepared coating agent for 2 - 4 s, then lift them up and place them in a cool place to dry.

9. The application of the moss gametophyte coating agent according to claim 8, characterized in that, The moss stem segment is any one of Racomitrium japonicum stem segments, Hypnum plumaeforme stem segments, Climacium dendroides stem segments, Plagiomnium acutum stem segments, Hypnum cupressiforme Hedw., Polytrichum commune Hedw., and Bryum argenteum Hedw. stem segments.

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