Fermented dry food for pets and method for preparing the same

Abstract

The application discloses a kind of pet fermented dry food, raw materials include according to weight fraction: fermented plant raw material 10~53 parts, animal meat source 10~60 parts and oil 5~15 parts;Wherein, the fermented plant raw material is the plant raw material fermented by probiotics.The application also discloses the preparation method of the above-mentioned pet fermented dry food.The application uses specific probiotics to ferment plant raw material in pet food, according to scientific proportion, completes animal protein, plant protein, functional plant raw material fermentation.After bulking process, realize the dry food product of good palatability of nutrition, safety, health, to meet the precise and special nutritional needs of special life cycle pet.The pet fermented dry food of the application is suitable for the digestive system of pet, promotes the digestion problem of protein in pet food, can reduce the burden of pet intestinal tract, realizes pet nutrition and health.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of pet food, and particularly relates to a fermented dry food for pets and a preparation method thereof. BACKGROUND

[0002] Pet food is the main source of nutrition for pets, and pets need different precise nutrition at different life cycles. The current design points of young pet food nutrition are high protein and high energy; the design points of old pet food nutrition are high-quality protein and appropriate energy. The raw materials used in existing pet main food are basically simple processed, and most of them are physically processed raw materials. These raw materials have certain characteristics, such as high protein content, low digestion and absorption rate, anti-nutritional factors in plant raw materials, and easy to cause pet intestinal discomfort. Through market research and pet owner feeding feedback, it can be seen that young pets and old pets generally have weak digestion, limited or decreased protein raw material digestion and absorption, sensitive and fragile intestinal tract, and are prone to food intolerance and intestinal problems such as food stress. Solving the problem of protein digestion and absorption in pet food can reduce the burden on the pet intestinal tract and achieve pet nutrition and health.

[0003] In order to solve the above problems, existing pet food usually directly adds digestive promoting components such as probiotics to the food. However, when the pet food is processed, especially heat processed, the probiotics are easy to be inactivated, and when ingested into the pet body, the vitality is damaged due to the special environment of the digestive tract, thereby affecting its efficacy. At present, there are single fermented raw materials on the market, and most of them are plant fermented raw materials. The requirements for microorganisms, the achievement of functional effects are not specific, and cannot achieve comprehensive effectiveness, precise design, and suitable pet intestinal fermentation products. In addition, although some pet foods use plant deep processing raw materials such as starch and plant separated protein as plant raw materials, they cannot actually solve the problem that too much plant raw material is ingested in the pet body, the intestinal tract is heavy, and cannot be fully digested and absorbed. At the same time, the price of plant deep processing products is high. Therefore, the fermentation pretreatment of raw materials can alleviate the burden on the pet intestinal tract, and the fermentation by-products can promote the growth and health of pets. SUMMARY

[0004] The present application provides a fermented dry food for pets and a preparation method thereof, which can effectively solve the problem of protein digestion and absorption in pet food, reduce the burden on the pet intestinal tract, and meet the precise and special nutritional needs of pets at special life cycles.

[0005] The specific technical solutions are as follows:

[0006] One of the purposes of the present application is to provide a fermented dry food for pets, which raw materials include, in terms of weight fraction, 10-53 parts of fermented plant raw materials, 10-60 parts of animal meat sources, and 5-15 parts of oil and fat.

[0007] The fermented plant raw materials are plant raw materials fermented by probiotics.

[0008] The present application ferments plant raw materials in advance, and the macromolecular substances such as proteins and plant fibers are changed into small molecular substances such as small peptides and amino acids after fermentation, so that the anti-nutritional substances in the plant raw materials are degraded, and the raw materials are more suitable for the utilization of the digestive tract of animals. At the same time, specific probiotics can produce active substances, such as rich enzymes, in addition to some starch, protein, and fat enzymes, and many non-starch enzymes that animals cannot synthesize themselves, such as cellulase, pectinase, and glucanase, which can effectively improve the growth performance of animals and improve the health of animals. The active ingredients produced during fermentation, such as exogenous digestive enzymes, cytokines, and functional polypeptides, can assist the action of the intestinal inherent digestive enzymes and help pets to digest and absorb nutrients after being eaten by pets. In addition, the probiotics introduced by the material and the bacterial protein produced by the probiotics themselves are also a kind of excellent protein, which has higher nutritional value than plant protein.

[0009] Further, the probiotics include animal lactobacillus, bacillus subtilis, lactobacillus plantarum, and lactobacillus acidophilus. The animal lactobacillus can be obtained by screening from pet feces. Preferably, the animal lactobacillus is animal lactobacillus LA4.

[0010] The animal homologous probiotics (animal lactobacillus), especially the homologous probiotics screened from dog and cat feces, are combined with the probiotics (bacillus subtilis, lactobacillus plantarum, and lactobacillus acidophilus) purified from other application products to ferment the plant raw materials, which can produce rich metabolic products, including enzymes, lactic acid, and immune factors. Experiments have proved that compared with single-source probiotics, the probiotic combination of the present application can significantly improve the digestive problems of pets.

[0011] Further, the probiotics further include at least one of lactobacillus fermentum and animal bifidobacterium. The lactobacillus fermentum and animal bifidobacterium can also be obtained by screening from the intestinal tract of pets.

[0012] Further, the fermented plant raw materials include fermented natural cereal raw materials such as corn and wheat, or / and plant by-products such as soybean meal and peanut meal.

[0013] Plant raw materials contain different proportions of anti-nutritional factors, such as protease inhibitors, phytic acid, lectins, erucic acid, gossypol, tannic acid, glucosinolate, etc., although a part of them will be destroyed in the product processing process, but they cannot be completely destroyed. Pre-treatment of the material can destroy the anti-nutritional factors to a greater extent. The present application uses a specific combination of probiotics to pre-ferment plant raw materials, making natural plant raw materials, especially grain raw materials, more suitable for pet digestion.

[0014] Further, the fermented plant raw material further comprises fermented Chinese medicinal plants or / and extracts thereof 0.5-3 parts by weight.

[0015] Preferably, the Chinese medicinal plants are at least one of Astragalus, Dioscorea, Atractylodes, Poria cocos, Portulaca oleracea, and Prunus mume.

[0016] Further, the animal meat source comprises enzymatically or / and hydrolytically treated animal meat source.

[0017] In addition to having better digestion and absorption effects, enzymatically or hydrolytically treated animal meat source can also increase flavor. In particular, for collagen-rich skin and cartilage, the treated skin and cartilage raw material is more easily digested and absorbed by pets, especially for senior dogs, and has more functional value. The proteins in the enzymatically treated animal meat source are broken down into oligopeptides, polypeptides, and amino acids, which can reduce the digestive burden of the pet's gastrointestinal tract and improve the absorption rate of nutrients. Fermented skin and cartilage raw materials can be completed by introducing probiotics for solid-state fermentation to optimize the raw materials.

[0018] Further, the preparation method of the enzymatically treated animal meat source is preferably as follows: the meat raw material is enzymatically treated at 45-60°C for 1-2h under the catalysis of protease to obtain the enzymatic product. The amount of protease is preferably 2´10 4 ~1´10 5 U / kg based on the total material to be enzymatically treated.

[0019] Specifically, the protease is preferably acid protease or / and alkaline protease.

[0020] Specifically, water can be added to the meat raw material to make the moisture content of the material reach 60wt%-80wt%.

[0021] Specifically, after the enzymatic treatment is completed, the temperature is increased to 95-105°C, and the enzyme is inactivated for 10-20min.

[0022] Further, the animal meat source can also include fermented skin and cartilage raw materials, such as fermented pigskin. Skin and cartilage raw materials are difficult to treat by enzymatic treatment and can be fermented.

[0023] Specifically, whey protein powder and sugars can be added to skin and cartilage raw materials, followed by inoculation with probiotics for fermentation. The processed skin and cartilage raw materials are more easily digested and absorbed by pets, especially senior dogs, and have greater functional value.

[0024] Specifically, in the fermentation of raw materials such as skin and cartilage, water can be added to the material to be fermented to make the moisture content of the material reach 60wt%~80wt%.

[0025] The amount of whey protein powder added is 1wt% to 2wt% based on the mass of the skin and cartilage raw materials; the preferred sugar is white granulated sugar, but other sugars may also be used, and the amount added is 3wt% to 8wt% based on the mass of the skin and cartilage raw materials.

[0026] In the fermentation of skin and cartilage raw materials, the inoculation amount of probiotics is preferably 1×10⁻⁶ per fermentation system. 7 ~1´10 10 CFU / mL.

[0027] In the fermentation of animal hides and cartilage, the probiotics are preferably *Lactobacillus plantarum* and *Lactobacillus acidophilus*. The preferred ratio of live *Lactobacillus plantarum* to *Lactobacillus acidophilus* is 1:(0.5~2). *Lactobacillus plantarum* and *Lactobacillus acidophilus* can be used for fermentation of animal hides and bones, improving the digestibility of animal by-products.

[0028] In the fermentation of raw materials such as skin and cartilage, the optimal fermentation conditions for probiotics are 30-37℃ for 3-8 hours. Sterilization is performed after the fermentation process.

[0029] Furthermore, the oil may be vegetable oil or / and animal oil.

[0030] The second objective of this invention is to provide a method for preparing the above-mentioned fermented dry pet food, characterized by comprising the following steps:

[0031] (1) Preparation of fermented plant raw materials: Inoculate probiotics into plant raw materials and ferment at 30~37℃ for 1~2h;

[0032] (2) Extrusion puffing: After the raw materials are mixed evenly, they are extruded and puffed to obtain puffed granules;

[0033] (3) Drying treatment: The puffed granules are dried.

[0034] The puffing process in the preparation method of this invention can inactivate probiotics and kill other microorganisms that may exist after the early fermented material is subjected to high temperature and high pressure, while completing protein denaturation, starch gelatinization, and enabling the auxiliary material to achieve good performance.

[0035] Further, in step (1), the amount of the probiotic bacteria is 1´10 7 ~1´10 10 CFU / mL.

[0036] Further, in step (1), the amount of the probiotic bacteria is 1´10

[0037] Further, in step (1), the amount of the probiotic bacteria is 1´10

[0038] Further, in step (1), the amount of the probiotic bacteria is 1´10

[0039] Further, in step (1), the plant raw material is pre-crushed, then water is added to the material to a final water content of 40wt%-60wt%, and then the probiotic bacteria are inoculated. The water is preferably sprayed.

[0040] Further, after the drying treatment of step (3) is completed, the puffed particles are subjected to a rolling coating treatment, at least one of oil, animal hydrolysate and animal enzymatic product is coated on the outside of the puffed particles, and good organoleptic properties, flavor, taste and nutrition of the product are achieved.

[0041] The beneficial effects of the present application are as follows:

[0042] The present application uses specific probiotic bacteria to ferment plant raw materials in pet food, and according to a scientific ratio, animal protein, plant protein and functional plant raw materials are fermented, the fermentation process is used to change the characteristics of pet food raw materials, so that good processing characteristics and nutritional characteristics are achieved. After the puffing process, a dry food product with good nutrition, safety, health and palatability is achieved to meet the precise and special nutritional needs of special life cycle pets. The pet fermented dry food of the present application is suitable for the digestive system of pets, promotes the digestion of protein in pet food, reduces the burden on the intestinal tract of pets, and achieves pet nutrition and health. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 WALTHAM dog feces scoring criteria in Test 1. DETAILED DESCRIPTION

[0044] The principles and characteristics of the present application are described below in conjunction with examples, which are used to explain the present application but not to limit the scope of the present application. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.

[0045] In the embodiments, the Lactobacillus animalis is Lactobacillus animalis LA4, the Lactobacillus fermentum is Lactobacillus fermentum CCM7421, the Bifidobacterium animalis is Bifidobacterium animalis V9, the Bacillus subtilis is Bacillus subtilis Pab02, the Lactobacillus plantarum is Lactobacillus plantarum P-8, and the Lactobacillus acidophilus is Lactobacillus acidophilus NCFM.

[0046] The above strains are provided by a commercially available probiotic company.

[0047] In the embodiments, the preparation method of the enzymatic animal meat source is as follows:

[0048] Water is added to the chicken meat to make the moisture content of the material reach 65wt%, 4´10 4 U / kg of alkaline protease is added, and enzymolysis is carried out at 55℃ for 1.5h, then the temperature is increased to 100℃, and the enzyme is inactivated for 20min to obtain the enzymatic animal meat source.

[0049] In the embodiments, the preparation method of the fermented pigskin is as follows:

[0050] 1wt% of whey protein powder and 3wt% of white granulated sugar are prepared according to the mass of the pigskin, sterilized at 100℃ for 10min, then the pigskin is added, and water is added to make the moisture content of the material reach 65wt%; 1´10 8 CFU / mL of probiotics is added, the probiotics are composed of Lactobacillus plantarum and Lactobacillus acidophilus with a viable count of 1:1; 37℃ culture is carried out for 5h, then sterilization is carried out to obtain the enzymatic animal meat source.

[0051] Example 1

[0052] A fermented dry food for pets, the raw materials of which include, in terms of weight fraction: fermented grains 35 parts (corn 15 parts, wheat 20 parts), fermented soybean meal 3.5 parts, fermented traditional Chinese medicine 1 part (Radix Astragali 0.3 part, Chinese yam 0.2 part, white atractylodes 0.2 part, Poria cocos 0.1 part, spiny amaranth 0.1 part, Fructus Mume 0.1 part), enzymatic animal meat source 40 parts, fermented pigskin 10 parts, animal fat 15 parts (chicken oil 10 parts, beef tallow 5 parts).

[0053] The above fermented dry food for pets is prepared by the following method:

[0054] (1) Fermentation of plant raw materials: grind the grains, soybean meal and traditional Chinese medicine raw materials to 80wt% through a 60 mesh sieve, and spray water to the final moisture content of the material is 60wt%; inoculate probiotics into the grains, soybean meal and traditional Chinese medicine raw materials, and ferment at 37°C for 2h; the probiotics are composed of animal lactobacillus, bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus, fermenting lactobacillus and animal bifidobacterium; in the probiotics, the ratio of the viable counts of animal lactobacillus, bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus, fermenting lactobacillus and animal bifidobacterium is 1:1:1:1:1:1; the total inoculation amount of probiotics is 1´10 8 CFU / mL in the fermentation system;

[0055] (2) Extrusion: mix the raw materials evenly, and then extrude and puff at 120°C and 2.0MPa to obtain puffed particles;

[0056] (3) Drying treatment: dry the puffed particles obtained in step (2) at 100±5°C until the moisture content is not more than 6wt%;

[0057] (4) Rolling coating treatment: roll coat the dried particles obtained in step (3) with 7±1wt% of oil and 5wt% of taste enhancer based on the weight of the dried particles.

[0058] Example 2

[0059] With reference to Example 1, the difference between Example 1 and the present example is that in step (1), the probiotics are composed of animal lactobacillus, bacillus subtilis, lactobacillus plantarum and lactobacillus acidophilus; in the probiotics, the ratio of the viable counts of animal lactobacillus, bacillus subtilis, lactobacillus plantarum and lactobacillus acidophilus is 1:1:1:1.

[0060] Comparative Example 1

[0061] With reference to Example 1, the difference between Example 1 and the present example is that in step (1), the probiotics do not contain animal lactobacillus; the total amount of probiotics remains unchanged.

[0062] Comparative Example 2

[0063] With reference to Example 1, the difference between Example 1 and the present example is that in step (1), the probiotics do not contain bacillus subtilis; the total amount of probiotics remains unchanged.

[0064] Test 1

[0065] The fermented dry food obtained in each example and each comparative example is used to feed pets together with ordinary complete pet food. The ordinary complete pet food is prepared according to the formula of Example 1, and the difference between Example 1 and the present example is that the unfermented grains, soybean meal and traditional Chinese medicine are used to replace the fermented grains, fermented soybean meal and fermented traditional Chinese medicine in equal amounts.

[0066] Animal experiment scheme:

[0067] Animal sample: each example or comparative example as an experimental group, 10 in each group, healthy adult teddy, half male and half female, body weight 6±0.5kg.

[0068] Animal feeding: without pre-feeding, directly feeding the experimental food, feeding once a day, the feeding amount of each dog is 150g / day, and the feeding is stopped after 20min each time, then the remaining experimental food weight is weighed to obtain the intake amount. Single cage feeding for 15 days, recording the daily food intake of each dog, fecal score, fecal quality and determining the in vivo digestibility of protein and fat. The experimental results are shown in Table 1. In Table 1, the scoring standard of dog fecal score is Figure 1 , Figure 1 WALTHAM dog fecal score, wherein the fecal score is 2 (Grade 2), and the fecal score is 2.5 (Grade 2.5) when there is a tendency of diarrhea.

[0069] Data statistics and analysis: the test data is represented by "average value ± standard deviation".

[0070] Table 1

[0071]

[0072] The experimental results show that after the fermented complete pet food is ingested by pet dogs, the digestion and absorption rate of protein and fat in the product can be significantly improved. Compared with the ordinary complete pet food without fermentation and each comparative example, the fecal score of the pet can be significantly improved by the present application.

[0073] Test 2

[0074] The raw materials with high content of anti-nutritional factors are analyzed, and the results of soybean meal are shown in Table 2.

[0075] Table 2

[0076]

[0077] The experimental results show that after high temperature, part of the anti-nutritional factors of soybean meal are removed, and there is no difference between trypsin inhibitor and fermented soybean meal. After fermentation, the anti-nutritional factors of soybean meal are more thoroughly removed, and the content of β-conglycinin is significantly reduced.

[0078] The above only describes the preferred embodiments of the present application, and is not used to limit the present application, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A fermented dry pet food, characterized in that, The raw materials, by weight, include: 39.5 parts fermented plant materials, 40 parts enzymatically hydrolyzed animal meat, 10 parts fermented pigskin, and 15 parts animal fat. The fermented plant-based raw materials consist of 35 parts fermented grains, 3.5 parts fermented soybean meal, and 1 part fermented traditional Chinese medicine; the fermented grains consist of 15 parts corn and 20 parts wheat; the fermented traditional Chinese medicine consists of 0.3 parts astragalus, 0.2 parts yam, 0.2 parts atractylodes macrocephala, 0.1 parts poria cocos, 0.1 parts purslane, and 0.1 parts dried plum. The animal fat is composed of 10 parts chicken fat and 5 parts beef fat. The fermented plant-based raw materials mentioned above are plant-based raw materials fermented by probiotics; The probiotics are composed of Lactobacillus animalis, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus fermentum and Bifidobacterium animalis; the ratio of the number of live bacteria of Lactobacillus animalis, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus fermentum and Bifidobacterium animalis is 1:1:1:1:1:1; The method for preparing the fermented dry pet food includes the following steps: (1) Preparation of fermented plant raw materials: Probiotics are inoculated into the plant raw materials and fermented at 30~37℃ for 1~2 hours; the amount of probiotics used is 1×10⁻⁶ per fermentation system. 8 CFU / mL; (2) Extrusion puffing: After the raw materials are mixed evenly, they are extruded and puffed to obtain puffed granules; (3) Drying treatment: The puffed granules are dried.

2. A method for preparing fermented dry pet food as described in claim 1, characterized in that, Includes the following steps: (1) Preparation of fermented plant raw materials: Probiotics are inoculated into the plant raw materials and fermented at 30~37℃ for 1~2 hours; the amount of probiotics used is 1×10⁻⁶ per fermentation system. 8 CFU / mL; (2) Extrusion puffing: After the raw materials are mixed evenly, they are extruded and puffed to obtain puffed granules; (3) Drying treatment: The puffed granules are dried.

3. The preparation method according to claim 2, characterized in that, After the drying process in step (3) is completed, the puffed granules are coated with at least one of the following: oil, animal hydrolysate, and animal enzymatic hydrolysate.

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