Traditional Chinese medicine oral liquid for promoting growth of livestock and poultry, preparation method and application thereof

Abstract

The invention discloses a Chinese medicine oral liquid for promoting growth of livestock and poultry, and its preparation method and application; it is mixed by Chinese medicine oral liquid A liquid and Chinese medicine oral liquid B liquid; the raw materials of the crude drugs of the Chinese medicine oral liquid A include hawthorn and astragalus; the raw materials of the crude drugs of the Chinese medicine oral liquid B include 45-55 parts of glycine, 45-55 parts of L-lysine, 45-55 parts of DL-methionine, 10-20 parts of L-arginine, 47-95 parts of multivitamins, 45-55 parts of DL-α-tocopherol acetate, and 1-10 parts of D-biotin. The Chinese medicine oral liquid of the present invention has good muscle-building and growth-promoting effects on livestock and poultry, especially can increase the growth rate of livestock and poultry, improve their disease resistance, and increase muscle content. The dosage form of the present invention is an oral liquid, which can be directly added to the drinking water of poultry for easy absorption.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine, and in particular to a traditional Chinese medicine oral liquid for promoting the growth of livestock and poultry, and a preparation method and application thereof. Background Art

[0002] The growth of livestock and poultry is closely related to people's living standards. The faster the growth of livestock and poultry, the lower the price of meat, eggs and milk in people's lives, and the higher the living standards of people. The growth promotion of livestock and poultry mainly involves adding some additives to the feed, just like the additives in people's food in life. Feed additives in the breeding industry can be divided into nutritional and non-nutritional feed additives. Non-nutritional feed additives can be divided into traditional additives and "green" feed additives. Traditional additives generally include antibiotics and hormones. "Green" feed additives generally include herbal additives, enzyme preparations, probiotics, acidulants, natural plant extracts, etc.

[0003] Antibiotics are a type of antibacterial growth-promoting additive that can be used to treat diseases caused by microbial infections, promote animal growth, and improve feed efficiency. However, there are also some disadvantages. If animals are fed antibiotics for a long time, the antibiotics may exist in the bodies of livestock and poultry for a long time. When processed or eaten by people, they may directly or indirectly harm people's health, cause chronic poisoning in humans, antibiotic resistance, and certain possible teratogenic, carcinogenic, and mutagenic risks. The European Union banned the use of antibiotic growth promoters in January 2006, causing panic among local consumers, but such drugs were not banned by the European Union. Instead, they were not allowed to be used as growth promoters, but could be used to prevent, control, and treat animal diseases. However, since the ban on antibiotics, no significant benefits have been found for humans. On the contrary, due to the ban on antibiotics, the incidence of animal diseases has increased, requiring a large amount of antibiotics as therapeutic drugs, which may cause people to take more antibiotics.

[0004] Traditional feed additives also include hormones, which can be divided into two categories: growth hormones and sex hormones. Growth hormones are a type of protein hormone with high safety. They can be digested but not absorbed, and have no drug residues. They can significantly promote the growth of beef cattle, but some people believe that proteins are easily decomposed by digestive enzymes and are not highly utilized as feed additives. Sex hormones can be used as feed additives to promote the growth of beef cattle, but the mechanism of action is not yet clear and requires further research. However, this type of hormone has a negative impact on the reproductive system and is now off the shelves.

[0005] Organic arsenic preparations can also be used as growth-promoting additives for livestock and poultry. Using them in broiler feed can increase feed utilization by 3% to 5%. Adding arsenic preparations can significantly change the color of animal skin and meat. Inorganic arsenic is converted into organic arsenic through methylation. When organic arsenic is used as a feed additive and fed to livestock and poultry, its excrement will exist in the environment, which will bring arsenic into the environment, doubling the arsenic content in the soil and groundwater, and causing immeasurable harm to the human body.

[0006] Traditional feed additives are more or less harmful to humans, so people want to find a green and harmless feed additive, and a new type of "green" feed additive has emerged. This additive is determined to effectively prevent the occurrence of livestock and poultry diseases, is conducive to growth, and has little toxicity and side effects, and no residue after feeding. Since Chinese herbal medicines are obtained from nature, they are pure and natural, have comprehensive biological activity, are highly safe, and will not produce drug resistance. Through the synergistic effect of different Chinese herbal medicines, various expected effects can be achieved and side effects can be reduced. Chinese herbal medicine resources are rich and the cost is low. Chinese herbal feed additives can enhance the immunity of livestock and poultry, inhibit bacteria and viruses, resist stress, and maintain the balance of intestinal microorganisms. They have broad development prospects in animal husbandry.

[0007] At present, the Chinese invention patent with the publication number CN103463544A discloses a poultry growth-promoting Chinese medicine oral liquid, which is composed of 15-30 parts of malt sprout, 5-15 parts of burnt betel nut, 10-20 parts of green peel, 10-20 parts of immature bitter orange, 20-40 parts of safflower, and 5-10 parts of amomum. However, the Chinese medicines such as betel nut, immature bitter orange, safflower, and amomum in the prescription do not belong to the 115 kinds of feedable Chinese herbal medicine additives, and are not suitable for long-term use. Among them, the price of amomum is high, and the prescription needs to take a higher content to have a growth-promoting effect. The overall price of the prescription is high, and the potential for practical application is low.

[0008] At present, the Chinese invention patent with publication number CN109198252A discloses a Chinese medicinal feed for growth-promoting broiler chickens, which is characterized by comprising the following raw materials in parts by weight: 5-15 parts by weight of onion, 4-18 parts by weight of garlic, 2-8 parts by weight of trace elements, 3-11 parts by weight of wormwood, 6-18 parts by weight of bentonite, 3-15 parts by weight of earthworm, 10-30 parts by weight of corn, 10-20 parts by weight of cooked rehmannia, 5-15 parts by weight of liquid enzyme preparation, and 10-20 parts by weight of fish. 8-25 weight parts of powder, 15-35 weight parts of bone meal, 20-30 weight parts of cottonseed cake, 10-15 weight parts of rapeseed cake, 15-30 weight parts of shellfish powder, 5-10 weight parts of salt, 4-18 weight parts of ophiopogon, 6-28 weight parts of fritillaria, 2-14 weight parts of pine needles, 3-13 weight parts of notopterygium, 4-18 weight parts of cordyceps, 3-13 weight parts of peony, 3-15 weight parts of asparagus, 4-18 weight parts of creeping plant root, 3-19 weight parts of buffalo horn. However, the Chinese medicines such as garlic, rehmannia, fritillaria, pine needles, etc. in the prescription do not belong to the 115 kinds of feedable Chinese herbal medicine additives, and are not suitable for long-term use. Among them, the prices of cordyceps and buffalo horns are high, and the potential for practical application is low.

[0009] Therefore, it is of great significance to develop low-cost, safe, non-toxic, growth-promoting livestock and poultry feed additives that meet feed additive standards. Summary of the invention

[0010] The purpose of the present invention is to overcome the deficiencies of the prior art and to provide a Chinese medicine oral liquid for promoting the growth of livestock and poultry, and a preparation method and application thereof.

[0011] To achieve the above purpose, the technical solution designed by the present invention is as follows:

[0012] The present invention provides a Chinese medicine oral liquid for promoting the growth of livestock and poultry, wherein the Chinese medicine oral liquid is prepared by mixing Chinese medicine oral liquid A liquid and Chinese medicine oral liquid B liquid;

[0013] The raw materials of the Chinese medicine oral liquid A include hawthorn and astragalus by weight;

[0014] The raw materials of the Chinese medicine oral liquid B include 500-1000 parts of astragalus, 45-55 parts of glycine, 45-55 parts of L-lysine, 45-55 parts of DL-methionine, 10-20 parts of L-arginine, 47-95 parts of complex vitamins, 45-55 parts of DL-α-tocopherol acetate, and 1-10 parts of D-biotin by weight.

[0015] Furthermore, in the crude drugs of the Chinese medicine oral liquid A, the weight ratio of hawthorn and astragalus is 2:1-5; the complex vitamin consists of 25-35 parts of vitamin A, 5-15 parts of vitamin B1, 5-15 parts of vitamin B2, 10-20 parts of vitamin B6, 1-10 parts of vitamin D3 and 1-10 parts of vitamin K3.

[0016] Furthermore, the Chinese medicine oral liquid is formed by mixing Chinese medicine oral liquid A liquid and Chinese medicine oral liquid B liquid;

[0017] The raw materials of the Chinese medicine oral liquid A include hawthorn and astragalus; and the weight ratio of hawthorn to astragalus is 2:1-5;

[0018] The raw materials of the crude drugs of the Chinese medicine oral liquid B include, by weight, 47-52 parts of glycine, 47-52 parts of L-lysine, 47-52 parts of DL-methionine, 12-17 parts of L-arginine, 27-32 parts of vitamin A, 8-13 parts of vitamin B1, 8-13 parts of vitamin B2, 12-17 parts of vitamin B6, 1-8 parts of vitamin D3, 3-8 parts of vitamin K3, 47-52 parts of DL-α-tocopherol acetate, and 1-8 parts of D-biotin.

[0019] Furthermore, the Chinese medicine oral liquid is prepared by mixing Chinese medicine oral liquid A and Chinese medicine oral liquid B in a volume ratio of 1:3-12; in the crude drugs of the Chinese medicine oral liquid A, the weight ratio of hawthorn and astragalus is 1:4.

[0020] Furthermore, the Chinese medicine oral liquid is prepared by mixing Chinese medicine oral liquid A and Chinese medicine oral liquid B in a volume ratio of 1:8;

[0021] The raw materials of the Chinese medicine oral liquid A include hawthorn and astragalus; and the weight ratio of hawthorn to astragalus is 1:4;

[0022] The raw materials of the crude drugs of the Chinese medicine oral liquid B include, by weight, 50 parts of glycine, 50 parts of L-lysine, 50 parts of DL-methionine, 15 parts of L-arginine, 30 parts of vitamin A, 10 parts of vitamin B1, 10 parts of vitamin B2, 15 parts of vitamin B6, 2 parts of vitamin D3, 3 parts of vitamin K3, 50 parts of DL-α-tocopherol acetate, and 1 part of D-biotin.

[0023] The present invention also provides a method for preparing the above-mentioned Chinese medicine oral liquid for promoting growth of livestock and poultry, comprising the following steps:

[0024] 1) Weigh hawthorn and astragalus according to the above weight ratio;

[0025] 2) Soak hawthorn and astragalus in water, heat over high heat until boiling, pour out the first decoction after boiling, add warm water and heat again to obtain the second decoction;

[0026] 3) mixing the two obtained medicinal solutions and concentrating them to obtain a Chinese medicine oral solution A having a crude drug content of 1 g / mL;

[0027] 4) Weigh glycine, L-lysine, DL-methionine, L-arginine, multivitamins, DL-α-tocopheryl acetate, D-biotin, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3 1 and vitamin K3 according to the above weight ratios.

[0028] 5) dissolving glycine, L-lysine, DL-methionine, L-arginine, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin K3, DL-α-tocopherol acetate, and D-biotin in deionized water to obtain Chinese medicine oral liquid B; wherein, in every 500g of Chinese medicine oral liquid B, glycine ≥5000mg / kg, L-lysine ≥5000mg / kg, DL-methionine ≥5000mg / kg, L-arginine ≥1500mg / kg, vitamin A 300,000-10,000,000 IU / kg, vitamin B1 ≥1000mg / kg, vitamin B2 ≥1000mg / kg, vitamin B6 ≥1500mg / kg, vitamin D3 1-6,000,000 IU / kg, vitamin K3 ≥300mg / kg, DL-α-tocopherol acetate ≥5000mg / kg, and D-biotin ≥100mg / kg;

[0029] 6) Mixing liquid A and liquid B in a volume ratio of 1:3-12 to obtain the Chinese medicine oral liquid.

[0030] The present invention also provides an application of the above-mentioned Chinese medicine oral liquid for promoting the growth of livestock and poultry in preparing a Chinese medicine preparation for promoting the growth of livestock and poultry.

[0031] The present invention also provides a traditional Chinese medicine preparation, which comprises the traditional Chinese medicine oral liquid according to any one of claims 1 to 5.

[0032] Furthermore, the traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

[0033] Furthermore, the auxiliary material is any one or more of vitamin C, sorbitol, mannitol, xylitol, fructose, amino acids, meglumine, dextrin, magnesium stearate and sucrose.

[0034] The pharmacological properties of the present invention are as follows:

[0035] The astragalus in the compound of the present invention has the effects of invigorating qi and improving immunity; the hawthorn has the effects of stimulating appetite, aiding digestion and improving feed utilization rate; and other amino acids and vitamins can balance nutrition.

[0036] Beneficial effects of the present invention:

[0037] 1. The Chinese medicinal compound of the present invention can promote the growth of mice, improve their lipid metabolism and digestive enzyme functions, and can also promote the growth of broilers and increase their muscle content.

[0038] 2. The Chinese medicine oral liquid of the present invention has no toxic side effects. The cost price of each 500g of the Chinese medicine oral liquid is 50 yuan, which can be used by 3000-5000 chickens for one month, and the average cost per chicken is 0.016-0.01 yuan. The oral liquid prepared by the prescription is fast in effect, easy to absorb, and simple to prepare.

[0039] 3. The main ingredients of the present invention, astragalus and hawthorn, belong to 115 feedable Chinese medicinal additives, comply with relevant policies of my country, and can be used as feed additives for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is a graph of mouse growth performance;

[0041] In the figure, A is the weight of mice in each group on day 0; B is the weight of mice in each group on day 14; C is the weight change of mice within 14 days; D is the feed-to-weight ratio change of mice within 14 days;

[0042] Blank, blank control group; F1, liquid A / liquid B = 1:5 (v / v); F2, liquid A / liquid B = 1:8 (v / v); F3, liquid A / liquid B = 1:10 (v / v).

[0043] Figure 2 This is the serum biochemical profile of mice 14 days after administration;

[0044] In the figure,

[0045] A is the serum biochemistry profile for Glu blood sugar (blood sugar (<14mmol / L));

[0046] B is the serum biochemical diagram of TG triglyceride (glycerol M (3.74+2.45);

[0047] C is the serum biochemical profile of TC total cholesterol (total cholesterol (6.39+5.26)

[0048] D is the serum biochemical profile of LDL-C (low-density lipoprotein cholesterol (2.73+2.37))

[0049] E is the serum biochemical profile of HDL-C high-density lipoprotein cholesterol (high-density lipoprotein cholesterol (2.01+1.28))

[0050] F is TBA bile acid content (bile acid (0-12));

[0051] Blank, blank control group; F1, liquid A / liquid B = 1:5 (v / v); F2, liquid A / liquid B = 1:8 (v / v); F3, liquid A / liquid B = 1:10 (v / v).

[0052] Figure 3 This is the change of digestive enzymes in the intestine of mice after 14 days of administration;

[0053] In the figure, AC are the activity diagrams of α-amylase, lipase and pepsin in the stomach respectively;

[0054] DF are the activity diagrams of α-amylase, lipase and pepsin in the duodenum;

[0055] Blank, blank control group; F1, liquid A / liquid B = 1:5 (v / v); F2, liquid A / liquid B = 1:8 (v / v); F3, liquid A / liquid B = 1:10 (v / v).

[0056] Figure 4 This is a broiler growth performance chart;

[0057] AC are the survival rate, average body weight and feed-to-weight ratio of broilers within 14 days;

[0058] DF are the survival rate, average body weight and feed-to-weight ratio of broilers within 28 days;

[0059] GI is the survival rate, average body weight and feed-to-weight ratio of broiler chickens within 42 days.

[0060] Group A, blank control group; Group B, 0.5% Chinese medicine oral liquid 2; Group C, 1% Chinese medicine oral liquid 2; Group D, 2% Chinese medicine oral liquid 2.

[0061] Figure 5 for broiler slaughter performance diagram;

[0062] A is the slaughter rate of broilers after 42 days of feeding;

[0063] B is the graph of leg muscle content of broiler chickens after 42 days of feeding;

[0064] Ten broilers in each group were slaughtered and analyzed using One-way ANOVA; *: P < 0.05; ns: P > 0.05. DETAILED DESCRIPTION

[0065] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand.

[0066] Example 1

[0067] The Chinese medicine oral liquid 1 for promoting growth of livestock and poultry is prepared by mixing Chinese medicine oral liquid A liquid and Chinese medicine oral liquid B liquid;

[0068] The raw materials of Chinese medicine oral liquid A are composed of 10g of hawthorn and 10g of astragalus;

[0069] The raw materials of the herbal medicine of Chinese medicine oral solution B are glycine 1000mg, L-lysine 1000mg, DL-methionine 1000mg, L-arginine 300mg, vitamin A 600mg (2 million IU), vitamin B1 200mg, vitamin B2 200mg, vitamin B6 300mg, vitamin D3 40mg (1.2 million IU), vitamin K3 60mg / kg, DL-α-tocopherol acetate 1000mg, and D-biotin 20mg.

[0070] The above-mentioned Chinese medicinal materials were purchased from Wuhan Zhihe Qingqiang Pharmacy Co., Ltd. and used in subsequent experiments after accurate weighing.

[0071] The preparation method of the above-mentioned Chinese medicine oral liquid 1 comprises the following steps:

[0072] 1) Weigh 20g each of hawthorn and astragalus, add 6 times water and soak for 15min. Heat in a digital display closed electric furnace, boil at 420℃, then heat at 300℃ for 20min, filter through 8 layers of sterile gauze to obtain the first decoction; add 6 times water to the remaining residue, boil at 420℃, then heat at 300℃ for 15min to obtain the second decoction, filter through 8 layers of sterile gauze to obtain the second decoction. Combine the two medicinal liquids and concentrate at 350℃ to obtain a Chinese medicine oral liquid A with a crude drug content of 1g / mL;

[0073] 2) Weigh 1000 mg of glycine, 1000 mg of L-lysine, 1000 mg of DL-methionine, 300 mg of L-arginine, 600 mg of vitamin A (2 million IU), 200 mg of vitamin B1, 200 mg of vitamin B2, 300 mg of vitamin B6, 40 mg of vitamin D3 (1.2 million IU), 60 mg / kg of vitamin K3, 1000 mg of DL-α-tocopheryl acetate, and 20 mg of D-biotin;

[0074] 3) Glycine, L-lysine, DL-methionine, L-arginine, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin K3, DL-α-tocopherol acetate, and D-biotin are prepared according to the ratio in claim 1 to obtain a Chinese medicine oral liquid B. In each 500g of liquid B, glycine ≥5000mg / kg, L-lysine ≥5000mg / kg, DL-methionine ≥5000mg / kg, L-arginine ≥1500mg / kg, vitamin A 300,000-10,000,000 IU / kg, vitamin B1 ≥1000mg / kg, vitamin B2 ≥1000mg / kg, vitamin B6 ≥1500mg / kg, vitamin D3 1-6,000,000 IU / kg, vitamin K3 ≥300mg / kg, DL-α-tocopherol acetate ≥5000mg / kg, and D-biotin ≥100mg / kg.

[0075] 4) Mix liquid A and liquid B of the oral solution in a volume ratio of 1:5, Chinese medicine oral solution 1.

[0076] Example 2

[0077] The Chinese medicine oral liquid 2 prepared in this example is basically the same as that in Example 1, except that:

[0078] Mix liquid A and liquid B of the oral solution in a volume ratio of 1:8.

[0079] Example 3

[0080] The Chinese medicine oral liquid 3 prepared in this example is basically the same as that in Example 1, except that:

[0081] Mix liquid A and liquid B of the oral solution in a volume ratio of 1:10.

[0082] Example 4

[0083] A traditional Chinese medicine preparation comprises a traditional Chinese medicine oral liquid 2 and auxiliary materials; the auxiliary materials are any one or more of vitamin C, sorbitol, mannitol, xylitol, fructose, amino acids, meglumine, dextrin, magnesium stearate and sucrose.

[0084] The oral Chinese medicine solutions prepared in Examples 1 to 3 above have the following effects:

[0085] 1. Verify the side effects of Chinese medicine oral solution 2

[0086] The Chinese medicine oral liquid 2 prepared in Example 1 was gavaged into mice at dosages of 250, 500, and 1000 mg / kg, and the median lethal dose of the mice was calculated 30 days after administration; the results showed that no mice died when the dosage was increased to 1000 mg / kg, and there was no significant difference in the food intake, water intake, body weight, and body weight growth rate between the experimental group and the control group. 50 Greater than 1000 mg / kg. This range far exceeds the commonly used effective dose of the prescription. This indicates that the compound has no side effects at an effective concentration.

[0087] 2. Preliminary verification of the effectiveness of Chinese medicine oral liquids 1 to 3

[0088] (1) Experimental Materials

[0089] 1.1 Drugs: Chinese herbal oral solution 1-3 is concentrated to normal saline using a rotary evaporator.

[0090] 1.2 Animals: Male Kunming mice (SPF grade), weighing 20 ± 2 g, were purchased from Huazhong Agricultural University, license number SCXK (E) 2020-0019, experimental unit use license number SYXK (E) 2019-0044. This experiment was approved by the Scientific Ethics Committee of Huazhong Agricultural University, ethics number HZAUMO-2022-0178. All mice were housed in an environment with a temperature of 22 ± 1 ° C and a humidity of 55 ± 5%. Maintain 12 hours of lighting / 12 hours of darkness, evenly irradiate natural light, 3 mice / cage, and mice can freely take food and water.

[0091] 1.3 Equipment: timer; 1mL syringe; mouse gavage needle; electronic balance, model JE502, Shanghai Puchun Measuring Instrument Co., Ltd.; digital display closed electric furnace, Changsha Mingjie Instrument Co., Ltd.; electric constant temperature blast drying oven, model DHG-9240, Shanghai Jinghong Experimental Equipment Co., Ltd.; fully automatic biochemical analyzer, model BS-240, Shenzhen Mindray Bio-Medical Electronics Co., Ltd.; constant temperature box, DHG-9240A, Shanghai Jinghong Experimental Equipment Co., Ltd.; centrifuge, LX-165T2R, Qingdao Hitech Bio-Medical Co., Ltd.; microplate reader, uQuant, Bio-Tek.

[0092] (2) Experimental methods

[0093] The experiment began after the mice were fed for 7 days of adaptation. During the feeding period, the mice were free to drink water and eat. The drugs were administered by gavage every day from 2 to 4 pm. 40 mice were divided into 4 groups, with 10 mice in each group. Blank group (Blank), Chinese medicine oral liquid group 1 (F1), Chinese medicine oral liquid group 2 (F2), and Chinese medicine oral liquid group 3 (F3). The drugs were administered continuously for 14 days. During the feeding period, the mice were weighed and the feed weight was weighed every two days, and the average feed-to-weight ratio* and daily weight gain were calculated. The growth-promoting performance of Chinese medicine oral liquids 1 to 3 was evaluated by analyzing the weight of the mice. The mice were killed by cervical dislocation on the 15th day, and the intestines were taken for digestive enzyme activity analysis after dissection, and the blood was taken for serum biochemical analysis.

[0094] (3) Experimental results

[0095] The results of 14-day administration showed that F1, F2 and F3 all had a growth-promoting effect, and the weight of mice increased significantly ( Figure 1 AC). By day 14, the average weight gain of mice in groups F1, F2, and F3 was 14.66 g, 14.46 g, and 14.3 g ( Figure 1 B). Compared with the blank control group, the feed-to-weight ratio of F2 and F3 groups was better ( Figure 1 D) These results suggest that compared with the blank control group, F1, F2 and F3 can better promote the growth of mice and improve the growth performance of mice.

[0096] The serum biochemical indexes of mice in the drug-treated group and the blank control group were all within the normal range (normal range is marked after the brackets in the figure). Compared with the blank control group, the blood glucose concentration of mice in each experimental group was higher ( Figure 2 A). In terms of lipid metabolism, compared with the blank control group, F2 and F3 in the Chinese medicine oral liquid group could significantly reduce TG levels ( Figure 2 B); Chinese medicine oral solution can significantly reduce the levels of TC, LCL-C and HDL-C ( Figure 2 CE). The bile acid level of the Chinese medicine oral liquid F1 was lower than that of the blank control group ( Figure 2 F). In conclusion, the above results suggest that Chinese herbal oral solution can improve glucose and lipid metabolism, promote sugar absorption, and reduce lipid accumulation in mice.

[0097] Chinese herbal oral liquid can increase the activity of amylase in the stomach and duodenum. Figure 3 The results showed the activity of amylase, lipase and protease in the intestine of mice after 14 days of administration. The results showed that Chinese medicine oral liquid F2 can significantly increase the activity of lipase in the stomach, which may be the reason why the blood lipid content of mice in the Chinese medicine oral liquid F2 group was lower.

[0098] 3. Further verify the effective concentration of Chinese medicine oral solution 2 in target animals:

[0099] (1) Experimental Materials

[0100] 1.1 Drugs: Chinese medicine oral solution 2.

[0101] 1.2 Animals: AA broiler chickens, 1 day old, 144, half male and half female, with no significant difference in body weight. Purchased from Henan Longhua Animal Husbandry Co., Ltd. This experiment was approved by the Scientific Ethics Committee of Huazhong Agricultural University, ethics number: HZAUCH-2023-0003. All broilers were kept in an environment with a temperature of 22±1℃ and a humidity of 55±5%. 12 chickens / cage, broilers can freely take food and water.

[0102] 1.3 Equipment: Rotary evaporator, Shanghai Shensheng Technology Co., Ltd.; electronic balance, model JE502, Shanghai Puchun Measuring Instrument Co., Ltd.; digital display closed electric furnace, Changsha Mingjie Instrument Co., Ltd.

[0103] (2) Experimental methods

[0104] According to Example 2, Chinese medicine oral liquid 2 was prepared. 180 1-day-old AA broiler chickens were randomly divided into 4 groups (A-D), with 3 replicates in each group and 12 chickens in each replicate. The blank control group (A) was given basic feed*; the experimental groups (B-D) added 0.5%, 1% and 2% of Chinese medicine oral liquid 2 to the drinking water, respectively. The experimental period was 42 days, divided into 3 stages of 1-14 days, 15-28 days and 29-42 days. Before feeding in the morning of the 14th, 28th and 42nd day of the experiment, the weight of each replicate chicken was weighed on an empty stomach. The weight and feed intake of the chickens before and after each replicate in the 3 stages were recorded, and the average daily feed intake, average daily weight gain and feed-to-weight ratio of the 3 stages of 1-14, 15-28 and 29-42 days were counted and calculated. Observe and record the death of the chickens every day, and deal with the death of chickens immediately. All broilers were killed on day 43 and then disposed of in a pollution-free manner. Before all broilers were killed, 10 broilers were selected from each group, slaughtered and gutted, and the right legs were skinned and weighed to calculate the broiler slaughter rate and leg muscle rate.

[0105] (3) Experimental results

[0106] Chinese medicine oral liquid 2 significantly improved the growth performance of broiler chickens. From the perspective of market rate (survival rate), Chinese medicine oral liquid 2 significantly improved the survival rate of broiler chickens on the 14th, 28th and 42nd days of administration ( Figure 4 A, D, G). On the last day of the experiment, the survival rates of broilers in the groups treated with 0.5%, 1% and 2% of Chinese medicine oral liquid 2 were 72.22%, 91.67% and 83.33%, respectively, while the survival rate of broilers in the blank control group was 53.60%. In terms of average body weight, there was no significant difference in the body weight of broilers in each group within 14 days ( Figure 4 B), but at 28 and 42 days, Chinese medicine oral liquid 2 showed obvious growth-promoting effect ( Figure 4E, H). On the 28th day, the body weight of broilers in groups B, C, and D increased by 37.21%, 23.40%, and 8.51% compared with the blank control group. On the 42nd day, the body weight of broilers in groups B, C, and D increased by 29.51%, 11.48%, and 8.20% compared with the blank control group. In terms of feed-to-weight ratio, there was no significant difference between the compound super energy group and the blank control group within 42 days.

[0107] Chinese medicine oral liquid 2 can significantly increase the muscle content of broiler chickens. After 42 days of feeding, there was no significant difference in the slaughter rate of broiler chickens among the groups ( Figure 5 A), but there was a significant difference in muscle content ( Figure 5 B). Compared with the blank control group, feeding with 0.5% of Chinese medicine oral liquid 2 significantly increased the leg muscle content of broilers by about 16.12%, but feeding with 1% and 2% of Chinese medicine oral liquid 2 reduced the leg muscle content of broilers. This suggests that the effective concentration of Chinese medicine oral liquid 2 should be 0.5% to evaluate the increase in muscle content of broilers.

[0108] In summary, the present invention can be used as a Chinese medicine oral liquid for promoting the growth of livestock and poultry.

[0109] Although the above embodiments have been described in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all belong to the protection scope of the present invention.

Claims

1. A Chinese medicine oral liquid for promoting the growth of livestock and poultry, characterized in that: The Chinese medicine oral liquid is prepared by mixing Chinese medicine oral liquid A and Chinese medicine oral liquid B in a volume ratio of 1:3-12; The raw materials of the Chinese medicine oral liquid A are hawthorn and astragalus, and the weight ratio of hawthorn to astragalus is 2:1-5; The raw materials of the Chinese medicine oral liquid B are calculated by weight as follows: 45-55 parts of glycine, 45-55 parts of L-lysine, 45-55 parts of DL-methionine, 10-20 parts of L-arginine, 47-95 parts of multivitamins, 45-55 parts of DL-α-tocopherol acetate, and 1-10 parts of D-biotin, wherein: The complex vitamin consists of 25-35 parts of vitamin A, 5-15 parts of vitamin B1, 5-15 parts of vitamin B2, 10-20 parts of vitamin B6, 1-10 parts of vitamin D3 and 1-10 parts of vitamin K3.

2. The Chinese medicinal oral liquid for promoting growth of livestock and poultry according to claim 1, characterized in that: The Chinese medicine oral liquid is prepared by mixing Chinese medicine oral liquid A liquid and Chinese medicine oral liquid B liquid; The raw materials of the Chinese medicine oral liquid A are hawthorn and astragalus; and the weight ratio of hawthorn to astragalus is 2:1-5; The raw materials of the crude drugs of the Chinese medicine oral liquid B are calculated by weight as follows: 47-52 parts of glycine, 47-52 parts of L-lysine, 47-52 parts of DL-methionine, 12-17 parts of L-arginine, 27-32 parts of vitamin A, 8-13 parts of vitamin B1, 8-13 parts of vitamin B2, 12-17 parts of vitamin B6, 1-8 parts of vitamin D3, 3-8 parts of vitamin K3, 47-52 parts of DL-α-tocopherol acetate, and 1-8 parts of D-biotin.

3. The Chinese medicinal oral liquid for promoting growth of livestock and poultry according to claim 1 or 2, characterized in that: In the crude drugs of the Chinese medicine oral liquid A, the weight ratio of hawthorn and astragalus is 1:

4.

4. The Chinese medicine oral liquid for promoting growth of livestock and poultry according to claim 3, characterized in that: The Chinese medicine oral liquid is prepared by mixing Chinese medicine oral liquid A and Chinese medicine oral liquid B in a volume ratio of 1:8; The raw materials of the Chinese medicine oral liquid A are hawthorn and astragalus; and the weight ratio of hawthorn to astragalus is 1:4; The raw materials of the crude drugs of the Chinese medicine oral liquid B are calculated by weight as follows: 50 parts of glycine, 50 parts of L-lysine, 50 parts of DL-methionine, 15 parts of L-arginine, 30 parts of vitamin A, 10 parts of vitamin B1, 10 parts of vitamin B2, 15 parts of vitamin B6, 2 parts of vitamin D3, 3 parts of vitamin K3, 50 parts of DL-α-tocopherol acetate and 1 part of D-biotin.

5. A method for preparing the Chinese medicine oral liquid for promoting growth of livestock and poultry according to claim 1, characterized in that: The following steps are involved: 1) Weigh hawthorn and astragalus; 2) Soak hawthorn and astragalus in water, heat over high heat until boiling, pour out the first decoction after boiling, add warm water and heat again to obtain the second decoction; 3) mixing the two obtained medicinal solutions, and concentrating them to obtain a Chinese medicine oral solution A having a total crude drug content of 1 g / mL; 4) Weigh glycine, L-lysine, DL-methionine, L-arginine, multivitamins, DL-α-tocopheryl acetate, D-biotin, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3 1 and vitamin K3 according to the above weight ratios. 5) dissolving glycine, L-lysine, DL-methionine, L-arginine, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin K3, DL-α-tocopherol acetate, and D-biotin in deionized water to obtain Chinese medicine oral liquid B; wherein, in every 500g of Chinese medicine oral liquid B, glycine ≥5000mg / kg, L-lysine ≥5000mg / kg, DL-methionine ≥5000mg / kg, L-arginine ≥1500mg / kg, vitamin A 300,000-10,000,000 IU / kg, vitamin B1 ≥1000mg / kg, vitamin B2 ≥1000mg / kg, vitamin B6 ≥1500mg / kg, vitamin D3 1-6,000,000 IU / kg, vitamin K3 ≥300mg / kg, DL-α-tocopherol acetate ≥5000mg / kg, and D-biotin ≥100mg / kg; 6) Mixing liquid A and liquid B in a volume ratio of 1:3-12 to obtain the Chinese medicine oral liquid.

6. Use of the Chinese medicinal oral liquid for promoting growth of livestock and poultry according to any one of claims 1 to 4 in the preparation of Chinese medicinal preparations for promoting growth of livestock and poultry.

7. A Chinese medicine preparation, characterized in that: The Chinese medicine preparation comprises the Chinese medicine oral liquid according to any one of claims 1 to 5.

8. The Chinese medicine preparation according to claim 7, characterized in that: The traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

9. The Chinese medicine preparation according to claim 8, characterized in that: The auxiliary material is any one or more of vitamin C, sorbitol, mannitol, xylitol, fructose, amino acids, meglumine, dextrin, magnesium stearate and sucrose.

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