A high-yield and high-quality cultivation method for lilies

Abstract

The present invention discloses a high-yield and high-quality cultivation method for lilies, belonging to the technical field of agricultural cultivation. The cultivation method includes: (1) soaking seeds with a biological bactericide for disinfection; (2) after deep plowing, jointly applying a soil conditioner and a biocontrol agent; (3) making high ridges and furrows for waterlogging prevention and control; (4) reasonably close planting to ensure the basic seedling number; (5) reasonably irrigating during the seedling emergence period and the budding period; (6) regulating and reasonably topdressing in stages. The present invention improves the planting method from the aspects of planting management. The high ridges and furrows for lilies solve the problems of waterlogging drainage and waterlogging reduction, reasonable close planting ensures the basic seedling number for high yield, the stage-by-stage regulation of topdressing promotes the cultivation of strong seedlings, and the combined prevention and control of a soil conditioner, a soil repair agent and a biocontrol agent improves the stress resistance and disease resistance effects, etc., which have long plagued the prominent problems of low yield and poor quality of fresh lilies, ensuring high yield and high quality of lilies and promoting large-scale planting.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural cultivation, and particularly relates to a high-yield and high-quality cultivation method for lilies. Background Art

[0002] Lily is a traditional crop with both medicinal and edible uses. As a local variety, Lilium brownii var. viridulum Baker has distinct characteristics and is one of the main fresh lilies in China. Lily is propagated by scales. During the long process of artificial and natural selection, the germplasm has formed a singleization of resistance sources and genetic germplasm. This singleization promotes the rapid reproduction of virulent races, accelerates the degradation of varieties and the weakening of disease resistance, making lilies vulnerable to pests and diseases throughout the growing season.

[0003] Traditional lily cultivation has very strict requirements for soil conditions, and is limited to cultivation in loose yellow soil, and the land needs to be changed every year. With the increasing market demand for lilies, in recent years, planting in field soil has become a trend, but planting in field soil has higher requirements for planting techniques. In the existing conventional lily planting, there are prominent problems such as early withering of seedlings, serious fusarium wilt on the bulbs, easy occurrence of disease spots on the scales, and difficult treatment of grass pests in the field, resulting in problems such as low continuous yield and poor quality in lily production (lilies with disease spots have no market value), leading to high costs and high risks and inability to form large-scale cultivation.

[0004] Therefore, it is an urgent problem to be solved at present to develop a high-yield and high-quality cultivation method that can greatly improve the yield of lilies and the integrity of planting and raising healthy bulbs. Summary of the Invention

[0005] The purpose of the present invention is to provide a high-yield and high-quality cultivation method for lilies to solve the problems existing in the above-mentioned prior art. The present invention improves the planting method from the aspects of planting management. The high-ridge bed making of lilies solves waterlogging and drainage, reasonable close planting ensures the basic seedling number for high yield, the staged regulation of topdressing promotes the cultivation of strong seedlings, and the combined prevention and control of soil conditioner, soil repair agent and biocontrol agent improves the stress resistance and disease resistance effects, etc., which have long troubled the prominent problems of low yield and poor quality of fresh lilies, ensuring high yield and high quality of lilies and promoting large-scale planting.

[0006] To achieve the above purpose, the present invention provides the following scheme:

[0007] The present invention provides a high-yield and high-quality cultivation method for lilies, which includes:

[0008] Soaking and disinfecting lily bulbs in a soaking solution;

[0009] Deeply plowing the soil and applying a soil conditioner;

[0010] Planting lilies, and applying a biocontrol agent and a soil repair agent during the seedling emergence period and budding period of lilies;

[0011] Among them, the seed soaking solution is a bacterial solution containing Bacillus methylotrophicus IBFCBF-2 and Bacillus velezensis YC5;

[0012] The soil conditioner is a mixed fertilizer containing Trichoderma harzianum and Bacillus velezensis LS8;

[0013] The biocontrol agent is a mixed bacterial agent containing Bacillus velezensis LS8, Bacillus velezensis YC5 and Bacillus methylotrophicus IBFCBF-2;

[0014] The Bacillus velezensis YC5 is preserved in the China General Microbiological Culture Collection Center on June 8, 2023, with the preservation number of CGMCC NO. 27583.

[0015] Furthermore, in the seed soaking solution, the mass ratio of Bacillus methylotrophicus IBFCBF-2 to Bacillus velezensis YC5 is 1 - 1.2:1.

[0016] Furthermore, in the soil conditioner, the mass ratio of Trichoderma harzianum to Bacillus velezensis LS8 is 1 - 1.5:1.

[0017] Furthermore, the soil remediation agent is mineral source fulvic acid potassium.

[0018] Furthermore, the mass ratio of the biocontrol agent to the soil remediation agent is 2 - 5:1.

[0019] Furthermore, in the biocontrol agent, the mass ratio of Bacillus velezensis LS8, Bacillus velezensis YC5 and Bacillus methylotrophicus IBFCBF-2 is 1.5:1.2:1.

[0020] Furthermore, the soil is field soil or yellow loam soil.

[0021] Furthermore, after deep - turning the soil and applying the soil conditioner, it also includes using organic fertilizer on the soil surface, and the dosage of the organic fertilizer is 300 - 500 kg / mu.

[0022] Furthermore, this method also includes top - dressing regulation during the seedling emergence period, budding period and winter fertilizer period of lily planting.

[0023] Furthermore, the top - dressing is carried out in stages according to the proportion that the winter fertilizer accounts for 50 wt%, the seedling emergence period accounts for 35 wt%, and the budding period accounts for 15 wt% after the total fertilization amount is determined.

[0024] The present invention discloses the following technical effects:

[0025] The present invention improves the utilization rate of fertilizers and pesticides by means of high-ridge bed making for precipitation, regulation of high-density planting population, and induction of plant stress resistance and disease resistance by fungal flora, ensuring high yield, high quality and high income in lily production.

[0026] In the seedling emergence stage and bud emergence stage of lilies, the present invention combines a biocontrol agent and a soil conditioner, significantly improving the yield and quality of lilies. The combination of Bacillus velezensis YC5 and other biocontrol agents can effectively improve the control effect on lily fusarium wilt. The synergistic effect of the selected bacterial agents of the present invention can achieve the purpose of disease resistance and high yield.

[0027] The cultivation method of the present invention has remarkable effects of increasing production and efficiency. The lilies are transparent white without spots, with good marketability, high yield and high quality. Compared with conventional sowing and cultivation, the planting density is increased by 30%, the amount of chemical fertilizer used is reduced by 20%, and the microbial bacterial agent partially replaces chemical pesticides, reducing the amount of chemical pesticides used by 30%. By adopting the cultivation method of the present invention, the yield per mu can reach 1900 - 2000 kg (more than 25% higher than conventional planting), the output value is as high as 30,000 - 40,000 yuan per mu, and the net income after deducting the cost is also more than 20,000 yuan. The economic benefit of planting lilies is very considerable. Description of the Drawings

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0029] Figure 1 It is the growth situation of lilies with high-ridge and high-density planting in the seedling stage;

[0030] Figure 2 It is a comparison diagram of the stem and leaf growth of lilies during the harvest period;

[0031] Figure 3 It is a comparison diagram of the root growth of lilies during the harvest period;

[0032] Figure 4 It is a comparison diagram of the lily bulbs during the harvest period. Detailed Embodiments

[0033] Now, various exemplary embodiments of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and implementation schemes of the present invention.

[0034] It should be understood that the terms used in this invention are only for describing specific embodiments and are not intended to limit the invention. Additionally, for the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0035] Unless otherwise noted, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0036] Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the specification of this invention, which are obvious to those skilled in the art. Other embodiments obtained from the specification of this invention are obvious to those skilled in the art. The specification and examples of this invention are merely exemplary.

[0037] Regarding the use of "comprising", "including", "having", "containing", etc. herein, they are all open-ended terms, meaning including but not limited to.

[0038] The strains used in the embodiments of the present invention include Bacillus velezensis YC5, Bacillus velezensis LS8, and Bacillus methylotrophicus IBFCBF-2. Among them, Bacillus velezensis YC5 was deposited at the China General Microbiological Culture Collection Center (abbreviated as CGMCC, address of the depositary institution: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; postal code: 100101) on June 8, 2023, with the deposit number CGMCC NO. 27583; Bacillus velezensis LS8 was deposited at the China General Microbiological Culture Collection Center (abbreviated as CGMCC, address of the depositary institution: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; postal code: 100101) on March 22, 2022, with the deposit number CGMCC NO. 24563, and Bacillus methylotrophicus IBFCBF-2 was deposited at the China General Microbiological Culture Collection Center (abbreviated as CGMCC, address of the depositary institution: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; postal code: 100101) on August 11, 2015, with the deposit number CGMCC NO. 11231.

[0039] Example 1

[0040] A high-quality and high-yield cultivation method for lilies:

[0041] 1. Bulb disinfection

[0042] Use Bacillus methylotrophicus IBFCBF-2 and Bacillus velezensis YC5, and prepare a bacterial solution with a final concentration of 10 8 cfu / mL according to the mass ratio of 1:1. Immerse the lily bulbs in the bacterial solution for 3 minutes and then air dry.

[0043] 2. Select a fertile plot with convenient drainage and field soil, and let it dry naturally in the sun. On a sunny day, use a rotary tiller without trenching to plow the land deeply twice.

[0044] 3. Soil disinfection: Mix Trichoderma harzianum (5500 g / mu) and Bacillus velezensis LS8 (3500 g / mu) evenly according to the mass ratio and use it as a base fertilizer, applying it in holes.

[0045] 4. Ridge making and waterlogging prevention: Apply 300 kg of organic fertilizer per mu to the surface of the soil for fine tillage to make ridges. The width of the ridge surface is 1.2 m, the height of the ridge is 30 cm, and the width of the furrow is 45 cm; sow appropriately deeper, with the planting ditch being 10 cm deep; lilies are afraid of waterlogging. During the rainy season and after heavy rains, ensure deep furrows and high ridges, with all furrows connected, and the field is dry when the rain stops; (see Figure 1 )

[0046] 5. Reasonable close planting to ensure the basic seedling number: The row and plant spacing for planting is 40 cm × 12 cm, with 11,000 plants planted per mu, and 400 kg of first-generation seed bulbs are required per mu; (see Figure 1 )

[0047] 6. Soil perfusion: During the two important periods of the emergence stage and the budding stage of lilies, a mixture of Bacillus spp. and mineral-source fulvic acid potassium is used for soil perfusion in a mass ratio of 2:1. The usage amounts of Bacillus velezensis LS8, Bacillus velezensis YC5, and Methylotrophic Bacillus IBFCBF-2 in the Bacillus spp. mixture are 1:1:1. After fermentation, the concentration of the bacteria is 2×10 10 cfu / mL, and the final application concentration is 10 8 cfu / mL, with a dosage of 500 mL per mu, and preventive control is carried out 2 times in advance;

[0048] Seize two key nodes such as the emergence stage and the budding stage of lilies: The diseases and pests of lilies using the "three controls" cultivation technology of controlling fertilizer, water, and diseases by this method are generally less severe. Combined with the application of microbial inoculants, the number of pesticide application times can be significantly reduced.

[0049] 7. Stage-by-stage regulation of topdressing: Reasonable topdressing is carried out 3 times. After determining the total amount of compound fertilizer applied, the fertilization amounts at each stage are determined according to the ratio of winter fertilizer accounting for 50%, emergence stage accounting for 35%, and budding stage accounting for 15%. Regulating topdressing in stages can effectively shift the application of nitrogen fertilizer backward, significantly reduce tillering fertilizer, control ineffective tillers, and ensure the main seedlings are strong.

[0050] Example 2

[0051] A high-quality and high-yield cultivation method for lilies:

[0052] 1. Seed bulb disinfection: Methylotrophic Bacillus IBFCBF-2 and Bacillus velezensis YC5 are mixed in a mass ratio of 1.2:1 to prepare a bacterial liquid with a final concentration of 10 8 cfu / mL. The lily seed bulbs are placed in the bacterial liquid and soaked for 4 minutes, then dried;

[0053] 2. Select a fertile plot with convenient drainage and field soil, naturally dry it in the sun, and use a rotary tiller without trenching to deeply till the soil 2 times on a sunny day;

[0054] 3. Soil disinfection: Trichoderma harzianum (5500 g / mu) and Bacillus velezensis LS8 (3500 g / mu) are mixed evenly and used as a base fertilizer, applied in furrows;

[0055] 4. Ridge making and waterlogging prevention: Apply 400 kg of organic fertilizer per mu on the soil surface for fine tillage to make ridges. The ridge surface is 1.2 m wide, the ridge height is 30 cm, and the furrow width is 45 cm; Sow at an appropriate depth, and the planting furrow is 10 cm deep; Lilies are afraid of waterlogging. During the rainy season and after heavy rains, ensure deep furrows and high ridges, with all furrows connected, and the field is dry when the rain stops;

[0056] 5. Reasonable close planting to ensure the basic seedling number: The row and plant spacing is 40 cm × 15 cm, and 9,000 plants are planted per mu. 350 kg of first-generation seed bulbs are required per mu;

[0057] 6. Soil perfusion: During the two important periods of the emergence stage and the budding stage of lilies, a mixed bacteria of Bacillus and fulvic acid potassium from mineral sources are used for soil perfusion in a mass ratio of 3:1. The usage amounts of Bacillus velezensis LS8, Bacillus velezensis YC5, and Bacillus methylotrophicus IBFCBF-2 in the mixed bacteria of Bacillus are 1.5:1:1. The concentration of the bacteria after fermentation is 2×10 10 cfu / mL, and the application final concentration is 10 8 cfu / mL. The dosage per mu is 800 mL for 3 times for early prevention and control;

[0058] 7. Staged regulation of topdressing: Reasonable topdressing is carried out 4 times. After determining the total fertilization amount of compound fertilizer, the fertilization amounts at each stage are determined according to the ratio that winter fertilizer accounts for 50%, emergence stage accounts for 35%, and budding stage accounts for 15%.

[0059] Example 3

[0060] A high-quality and high-yield cultivation method for lilies:

[0061] 1. Seed bulb disinfection: Using Bacillus methylotrophicus IBFCBF-2 and Bacillus velezensis YC5, in a mass ratio of 1:1, to prepare a bacterial solution with a final concentration of 10 8 cfu / mL. Put the lily seed bulbs into the bacterial solution and soak for 5 minutes, then dry them;

[0062] 2. Select a fertile plot with convenient drainage and yellow loam soil, naturally dry it, and use a rotary tiller without ditching to deeply till the soil 2 times on a sunny day;

[0063] 3. Soil disinfection: Mix Trichoderma harzianum (5,500 g / mu) and Bacillus velezensis LS8 (3,500 g / mu) evenly according to the mass ratio and use them as base fertilizer, apply them by hole application;

[0064] 4. Ridging and making beds for waterlogging prevention and control: Apply 500 kg of organic fertilizer per mu on the soil surface for fine tillage and make beds. The width of the bed surface is 1.2 m, the ridge height is 30 cm, and the width of the furrow is 45 cm; Sow appropriately deeper, and the planting ditch is 10 cm deep; Lilies are afraid of waterlogging. In the rainy season and after heavy rains, ensure deep furrows and high beds, with all furrows connected, and the field dry after the rain stops;

[0065] 5. Reasonable close planting to ensure the basic seedling number: The row and plant spacing is 40 cm × 13 cm, and 10,110 plants are planted per mu. 390 kg of first-generation seed bulbs are required per mu;

[0066] 6. Soil perfusion: During the two important periods of the emergence stage and the budding stage of lilies, a mixed bacterium of Bacillus and fulvic acid potassium from mineral sources is used for soil perfusion in a mass ratio of 5:1. The usage amounts of Bacillus velezensis LS8, Bacillus velezensis YC5, and Bacillus methylotrophicus IBFCBF-2 in the mixed bacterium of Bacillus are 1.5:1.2:1. The concentration of the bacteria after fermentation is 2×10 10 cfu / mL, and the final application concentration is 10 8 cfu / mL. The dosage per mu is 1000 mL, and it is used for early prevention and control 2 times;

[0067] 7. Staged regulation of topdressing: Reasonably topdress 3 times. After determining the total fertilization amount of compound fertilizer, determine the fertilization amount at each stage according to the ratio that winter fertilizer accounts for 50%, emergence stage accounts for 35%, and budding stage accounts for 15%.

[0068] Comparative Example 1

[0069] Same as Example 1, except that in step 5, the row spacing and plant spacing are 40 cm × 20 cm, and 7217 plants are planted per mu.

[0070] Comparative Example 2

[0071] Same as Example 1, except that in step 2, yellow loam soil is selected for planting, and in step 5, the row spacing and plant spacing are 40 cm × 21 cm, and 7082 plants are planted per mu.

[0072] Comparative Example 3

[0073] Same as Example 1, except that in step 2, yellow loam soil is selected for planting, in step 5, the row spacing and plant spacing are 40 cm × 20 cm, and 7276 plants are planted per mu. In step 1, the seed bulbs are disinfected by soaking with common agents (prepared with 200 - 400 mg / L of prochloraz: 100 - 400 mg / L of hymexazol: 50 - 100 mg / L of imidacloprid).

[0074] Comparative Example 4

[0075] Same as Example 1, except that in step 5, the row spacing and plant spacing are 40 cm × 20.58 m, and 7150 plants are planted per mu. In step 1, the seed bulbs are disinfected by soaking with common agents (prepared with 200 - 400 mg / L of prochloraz: 100 - 400 mg / L of hymexazol: 50 - 100 mg / L of imidacloprid).

[0076] Comparative Example 5

[0077] Same as Example 1, except that in step 1, the seed bulbs are disinfected by soaking with common agents (prepared with 200 - 400 mg / L of prochloraz: 100 - 400 mg / L of hymexazol: 50 - 100 mg / L of imidacloprid).

[0078] Comparative Example 6

[0079] Same as Example 1, except that in Step 3, Trichoderma harzianum is 3500 g / mu and Bacillus velezensis LS8 is 5500 g / mu.

[0080] Comparative Example 7

[0081] Same as Example 1, except that in Step 6, the usage amounts of Bacillus velezensis LS8, Bacillus velezensis YC5, and Bacillus methylotrophicus IBFCBF-2 are 1:1.5:1.2.

[0082] Comparative Example 8

[0083] Same as Example 1, except that in Step 1, only Bacillus methylotrophicus IBFCBF-2 is formulated into a bacterial liquid with a final concentration of 10 8 cfu / mL for seed soaking; in Step 6, the mixed Bacillus bacteria only include Bacillus velezensis LS8 and Bacillus methylotrophicus IBFCBF-2, where LS8:IBFCBF-2 = 2:1.

[0084] Comparative Example 9

[0085] Same as Example 1, except that in Step 1, Bacillus methylotrophicus IBFCBF-2 and Bacillus amyloliquefaciens IBFCBF-1 (deposit number CGMCC NO.11230) are formulated into a bacterial liquid with a final concentration of 10 8 cfu / mL for seed soaking; in Step 6, the mixed Bacillus bacteria are Bacillus velezensis LS8, Bacillus amyloliquefaciens IBFCBF-1, and Bacillus methylotrophicus IBFCBF-2, where LS8:IBFCBF-1:IBFCBF-2 = 1:1:1.

[0086] Test Example

[0087] The lilies were planted using the methods of Examples 1-3 and Comparative Examples 1-9. The lilies selected for the test were the second-generation Longya lilies, and the test site was Tengtian Town, Yongfeng County, Ji'an City, Jiangxi Province. The test planting areas were set in field soil and yellow loam respectively. The soil fertility of the two kinds of soil used in the test was the same, and the planting environment was the same.

[0088] The appearance quality and yield of the lilies are shown in Table 1. The comparison of photos of high-yield and high-quality lily cultivation and conventional planting is as Figures 2-4As shown: The lily plants cultivated by the high-yield and high-quality cultivation method have a neat plant type, thick and well-developed roots, and tightly clustered bulbs. At the harvest stage, they still show "strong seedlings, green leaves, white bulbs, and excellent quality"; while the lilies planted conventionally have weak roots, early withering of plants, and the bulbs are prone to produce blight spots, resulting in low yield, poor product quality, and even no marketability.

[0089] Table 1 Planting situations in each test plot

[0090]

[0091] The above results show that the cultivation method of the present invention mainly controls precipitation through high-ridge cultivation, regulates the density of the planting population, induces plant stress resistance and disease resistance by beneficial bacterial and fungal flora, improves the utilization rate of fertilizers and pesticides, and ensures high yield, high quality and high income in lily production.

[0092] The soil conditioner can have a significant effect on controlling lily blight during both the flowering period and the bulb swelling period. In addition, the yield and quality of lilies increase after the combined application of the soil conditioner and the biocontrol agent. The combined application of Bacillus velezensis YC5 and other biocontrol bacteria can effectively improve the control effect on lily blight. The synergistic effect of the drugs selected in the present invention can achieve the purpose of disease resistance and high yield.

[0093] The present invention has very significant effects in increasing production and efficiency. The lilies are white and spotless, have good marketability, and are of high yield and high quality. The pests and diseases of lilies using the "three-control" cultivation technology of controlling fertilizer, water, and disease are generally less severe. Compared with the conventional sowing and cultivation, the planting density of the high-yield water and fertilizer precise regulation technology for densely planted lilies is increased by 30%, the amount of chemical fertilizer used is reduced by 20%, the microbial inoculant partially replaces chemical pesticides, and the amount of chemical pesticides used is reduced by 30%. Moreover, during the production process, the amount of fertilizer and pesticide is reduced, the acidic soil is adjusted, the soil structure is improved, the occurrence of major diseases is inhibited, and the yield and quality of lilies are significantly improved. By using the cultivation method of the present invention, the yield per mu can reach 1900 - 2000 kg (more than 25% higher than conventional planting), the output value is as high as 30,000 - 40,000 yuan per mu, and the net income after deducting the cost is more than 20,000 yuan. The economic benefits of planting lilies are very considerable. Large-scale planting can be quickly realized.

[0094] The embodiments described above are only used to describe the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention should fall within the protection scope determined by the claims of the present invention.

Claims

1. A high-yield and high-quality cultivation method for lilies, characterized in that, The method includes: Soaking and disinfecting lily bulbs in a soaking solution; Deep plowing the soil and applying a soil conditioner; Planting lilies, and applying a biocontrol agent and a soil remediation agent during the seedling emergence stage and the budding stage of lilies; Wherein, the soaking solution is a bacterial solution containing Bacillus methylotrophicus IBFCBF-2 and Bacillus velezensis YC5; The soil conditioner is a mixed fertilizer containing Trichoderma harzianum and Bacillus velezensis LS8; The biocontrol agent is a mixed bacterial agent containing Bacillus velezensis LS8, Bacillus velezensis YC5 and Bacillus methylotrophicus IBFCBF-2; Bacillus velezensis YC5 is preserved in the China General Microbiological Culture Collection Center, the preservation time is June 8, 2023, and the preservation number is CGMCC NO.27583; Bacillus methylotrophicus IBFCBF-2 is preserved in the China General Microbiological Culture Collection Center, the preservation time is August 11, 2015, and the preservation number is CGMCC NO.11231; Bacillus velezensis LS8 is preserved in the China General Microbiological Culture Collection Center, the preservation time is March 22, 2022, and the preservation number is CGMCC NO.24563.

2. The cultivation method according to claim 1, wherein In the soaking solution, the mass ratio of Bacillus methylotrophicus IBFCBF-2 to Bacillus velezensis YC5 is 1-1.2:

1.

3. The cultivation method according to claim 1, characterized in that, In the soil conditioner, the mass ratio of Trichoderma harzianum to Bacillus velezensis LS8 is 1-1.5:

1.

4. The cultivation method according to claim 1, characterized in that, The soil remediation agent is mineral source fulvic acid potassium.

5. The cultivation method according to claim 1, characterized in that, The mass ratio of the biocontrol agent to the soil remediation agent is 2-5:

1.

6. The cultivation method according to claim 1, characterized in that, In the biocontrol agent, the mass ratio of Bacillus velezensis LS8, Bacillus velezensis YC5 and Bacillus methylotrophicus IBFCBF-2 is 1.5:1.2:

1.

7. The cultivation method according to claim 1, characterized in that, The soil is field soil.

8. The cultivation method according to claim 1, characterized in that, After deep plowing the soil and applying the soil conditioner, it also includes applying organic fertilizer on the soil surface, and the dosage of the organic fertilizer is 300-500 kg / mu.

9. The cultivation method according to claim 1, characterized in that, The method also includes topdressing regulation during the seedling emergence stage, budding stage and winter fertilizer stage of lily planting.

10. The cultivation method according to claim 9, wherein, The topdressing is carried out in stages according to the proportion that the winter fertilizer accounts for 50wt%, the seedling emergence stage accounts for 35wt%, and the budding stage accounts for 15wt% after the total fertilization amount is determined.

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