Jatropha leaf composite disinfectant and preparation method thereof

Abstract

The present application provides a Jatropha curcas leaf composite disinfectant and a preparation method thereof, and belongs to the technical field of bio-chemical industry. The present application specifically provides a composition with antibacterial and disinfecting effects, which is prepared from the following raw materials in the following weight ratio: 10-20 parts of Jatropha curcas leaf and 0.1-2 parts of single-chain quaternary ammonium salt surfactant. The composition can be used to prepare the Jatropha curcas leaf composite disinfectant. The Jatropha curcas leaf composite disinfectant prepared by the present application has broad-spectrum antibacterial effect, and plays a synergistic antibacterial effect compared with the Jatropha curcas leaf extract and the single-chain quaternary ammonium salt surfactant alone. Moreover, the Jatropha curcas leaf composite disinfectant has high safety to human body and causes little environmental pollution when used. As an antibacterial and disinfecting product, it has a good application prospect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of bio-chemical industry, and particularly relates to a Jatropha curcas leaf composite disinfectant and a preparation method thereof. BACKGROUND

[0002] The surface of an object is the most direct contact surface of human living environment. In infectious diseases, bacteria and virus pollution on the surface of an object or the environment often causes the diseases. Therefore, object surface disinfection is extremely important in the field of public health. Currently, commonly used antibacterial disinfectants are chemical disinfectants such as chlorine disinfectants, peroxide disinfectants, aldehyde disinfectants, alcohol disinfectants, etc. Long-term and large-scale use of a certain chemical antibacterial disinfectant can easily cause bacterial drug resistance. In addition, these chemical disinfectants have irritancy and corrosivity, which can cause harm to the human body to a certain extent. Furthermore, chemical disinfectants have a great impact on the environment and cannot achieve green environmental protection.

[0003] With the increasing attention to health and environment, more green and safe plant extract disinfectants have been developed. Many plant extracts have bactericidal and disinfecting effects, such as extracts of spilanthes acmella, extracts of cinnamomum cassia, extracts of syzygium aromaticum, extracts of lemon grass, etc. The use of natural plant extracts as bactericidal disinfectants has the advantages of safety, non-toxicity, weak irritancy, and little or no environmental pollution. However, the bactericidal and disinfecting effects of most plant extracts are weak, and the practical application range is narrow.

[0004] Jatropha curcas, commonly known as Jatropha, is a small tree or shrub of the Euphorbiaceae family. The leaves of Jatropha curcas contain a large amount of phenolic compounds and have certain antibacterial and disinfecting effects. A Jatropha curcas-derived bacteriostatic agent is disclosed in Chinese Patent No. CN102132708A. The patent uses fresh Jatropha curcas leaf water extract as the main active ingredient and adds surfactants as adjuvants to prepare a bacteriostatic agent with good antibacterial and disinfecting effects. However, the antibacterial and disinfecting effects of Jatropha curcas extract are limited. Therefore, it is of great significance to develop Jatropha curcas leaf extract composite disinfectant with better antibacterial and disinfecting effects to reduce the use of chemical disinfectants and protect the environment and human health. SUMMARY

[0005] The present application aims to provide a Jatropha curcas leaf composite disinfectant and a preparation method thereof.

[0006] The present application provides a composition with antibacterial and disinfecting effects, which is prepared from the following raw materials in the following weight ratio:

[0007] Jatropha curcas leaves 10-20 parts, and a single-chain quaternary ammonium salt surfactant 0.1-2 parts.

[0008] Further, the aforementioned composition is prepared from the following raw materials in the following weight ratio:

[0009] Jatropha curcas leaves 10 parts, single-chain quaternary ammonium salt surfactant 0.8-1.1 parts.

[0010] Further, the aforementioned composition is obtained by mixing the Jatropha curcas leaves extract obtained by extracting Jatropha curcas leaves with water or organic solvent with single-chain quaternary ammonium salt surfactant; in the composition obtained after mixing, the concentration of Jatropha curcas leaves crude drug is 1%, and the concentration of single-chain quaternary ammonium salt surfactant is 0.08-0.11%.

[0011] The concentration of 1% of Jatropha curcas leaves crude drug means that 1 g of Jatropha curcas leaves is used to prepare 100 mL of the composition; the concentration of 0.08-0.11% of single-chain quaternary ammonium salt surfactant means that 0.08-0.11 g of single-chain quaternary ammonium salt surfactant is contained in 100 mL of the composition.

[0012] Further, the Jatropha curcas leaves extract is obtained by extracting Jatropha curcas leaves with water;

[0013] Preferably, the preparation method of the Jatropha curcas leaves extract comprises the following steps: taking Jatropha curcas leaves, extracting with boiling water, filtering, and concentrating to obtain the Jatropha curcas leaves extract;

[0014] More preferably, the Jatropha curcas leaves are crushed and sieved before extraction;

[0015] And / or, the water is added in an amount of 20-35 times the weight of the Jatropha curcas leaves;

[0016] And / or, the extraction time is 30-60 minutes;

[0017] And / or, the concentration of Jatropha curcas leaves crude drug in the Jatropha curcas leaves extract is 10%.

[0018] The concentration of 10% of Jatropha curcas leaves crude drug in the Jatropha curcas leaves extract means that 10 g of Jatropha curcas leaves is used to prepare 100 mL of the Jatropha curcas leaves extract.

[0019] Further, the single-chain quaternary ammonium salt surfactant is benzalkonium chloride or benzalkonium bromide.

[0020] The application also provides a preparation method of the aforementioned composition, which comprises the following steps:

[0021] (1) taking Jatropha curcas leaves, extracting with boiling water, filtering, and concentrating to obtain the Jatropha curcas leaves extract;

[0022] (2) dissolving single-chain quaternary ammonium salt surfactant in water to obtain a single-chain quaternary ammonium salt surfactant solution;

[0023] (3) mixing the Jatropha curcas leaves extract and the single-chain quaternary ammonium salt surfactant solution to obtain the composition.

[0024] Further,

[0025] In step (1), the Jatropha curcas leaves are crushed and sieved before extraction;

[0026] In step (1), the water added is 20-35 times the weight of the Jatropha curcas leaves;

[0027] In step (1), the extraction time is 30-60 minutes;

[0028] In step (1), the concentration of Jatropha curcas leaves in the concentrated solution is 10%.

[0029] The present application also provides the use of the aforementioned composition in the preparation of an antibacterial agent.

[0030] Preferably, the antibacterial agent is resistant to Gram-negative bacteria and / or Gram-positive bacteria.

[0031] More preferably, the antibacterial agent is resistant to Staphylococcus aureus and / or Escherichia coli.

[0032] More preferably, the antibacterial agent is an antibacterial disinfectant.

[0033] The present application also provides a Jatropha curcas leaf composite disinfectant comprising the aforementioned composition and water.

[0034] Further, the Jatropha curcas leaf composite disinfectant contains 10-20 g of Jatropha curcas leaves, 0.1-2 g of single-chain quaternary ammonium salt surfactant, and the rest is water per 1000 mL.

[0035] Preferably, the Jatropha curcas leaf composite disinfectant contains 10 g of Jatropha curcas leaves, 0.8-1.1 g of single-chain quaternary ammonium salt surfactant, and the rest is water per 1000 mL.

[0036] The disinfectant of the present application can be used for surface disinfection of any object. The plant extract and surfactant selected by the present application are both water-soluble, chemically stable, and non-toxic.

[0037] Compared with the prior art, the present application has the following advantages:

[0038] The jatropha leaf composite disinfectant prepared by the jatropha leaf extract and the single-chain quaternary ammonium salt surfactant composition has broad-spectrum antibacterial effect, and the antibacterial effect is excellent, and the synergistic antibacterial effect is exerted compared with the jatropha leaf extract and the single-chain quaternary ammonium salt surfactant alone. Moreover, the jatropha leaf composite disinfectant is mainly derived from natural plants, and the content of the single-chain quaternary ammonium salt surfactant is extremely small, and the safety to human body is high when used, and the environmental pollution is extremely small. In addition, the jatropha leaf composite disinfectant uses jatropha leaf extraction, and the production process is simple, and there is no any pollutant, the utilization rate of the jatropha leaf is improved, and the jatropha leaf composite disinfectant is green and environment-friendly, the product obtained is stable, and as an antibacterial disinfectant product, the jatropha leaf composite disinfectant has good application prospect.

[0039] Obviously, according to the above content of the present application, according to the ordinary technical knowledge and means in the art, other various forms of modification, replacement or change can be made without departing from the above basic technical idea of the present application.

[0040] The above content of the present application will be further described in detail through the specific embodiments in the form of examples. However, this should not be understood as the scope of the above subject matter of the present application being limited to the following examples. Any technology realized based on the above content of the present application belongs to the scope of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0041] Figure 1 Inhibition of S. aureus over time for different samples.

[0042] Figure 2 Inhibition of E. coli over time for different samples.

[0043] Figure 3 Inhibition of S. aureus over time for different samples.

[0044] Figure 4 Inhibition of E. coli over time for different samples. DETAILED DESCRIPTION

[0045] The raw materials and equipment used in the specific embodiments of the present application are known products, which are obtained by purchasing commercially available products.

[0046] When the jatropha leaf composite disinfectant is prepared in the embodiments of the present application, the temperature and humidity control conditions of the production workshop are as follows: the temperature is room temperature, and the relative humidity is 30-60%.

[0047] Example 1, preparation of the jatropha leaf composite disinfectant of the present application

[0048] Jatropha curcas leaves were crushed to pass through a 60-mesh sieve, 10 g of which was added to 20 times the amount of pure water, boiled and extracted for 30 minutes, filtered, concentrated, and 100 ml of concentrated solution was obtained. 0.8 g of single-chain quaternary ammonium salt surfactant benzalkonium chloride was dissolved in 900 ml of boiling water to obtain a surfactant solution. The concentrated solution of Jatropha curcas leaves and the surfactant solution were stirred and mixed evenly while hot to obtain 1000 ml of Jatropha curcas leaf composite disinfectant. The concentration of quaternary ammonium salt surfactant in the disinfectant was 0.08%.

[0049] Example 2, Preparation of Jatropha curcas leaf composite disinfectant of the present application

[0050] Jatropha curcas leaves were crushed to pass through a 60-mesh sieve, 10 g of which was added to 25 times the amount of pure water, boiled and extracted for 40 minutes, filtered, concentrated, and 100 ml of concentrated solution was obtained. 0.9 g of single-chain quaternary ammonium salt surfactant benzalkonium chloride was dissolved in 900 ml of boiling water to obtain a surfactant solution. The concentrated solution of Jatropha curcas leaves and the surfactant solution were stirred and mixed evenly while hot to obtain 1000 ml of Jatropha curcas leaf composite disinfectant. The concentration of quaternary ammonium salt surfactant in the disinfectant was 0.09%.

[0051] Example 3, Preparation of Jatropha curcas leaf composite disinfectant of the present application

[0052] Jatropha curcas leaves were crushed to pass through a 60-mesh sieve, 10 g of which was added to 30 times the amount of pure water, boiled and extracted for 50 minutes, filtered, concentrated, and 100 ml of concentrated solution was obtained. 1.0 g of single-chain quaternary ammonium salt surfactant benzalkonium chloride was dissolved in 900 ml of boiling water to obtain a surfactant solution. The concentrated solution of Jatropha curcas leaves and the surfactant solution were stirred and mixed evenly while hot to obtain 1000 ml of Jatropha curcas leaf composite disinfectant. The concentration of quaternary ammonium salt surfactant in the disinfectant was 0.1%.

[0053] Example 4, Preparation of Jatropha curcas leaf composite disinfectant of the present application

[0054] Jatropha curcas leaves were crushed to pass through a 60-mesh sieve, 10 g of which was added to 35 times the amount of pure water, boiled and extracted for 60 minutes, filtered, concentrated, and 100 ml of concentrated solution was obtained. 1.1 g of single-chain quaternary ammonium salt surfactant benzalkonium chloride was dissolved in 900 ml of boiling water to obtain a surfactant solution. The concentrated solution of Jatropha curcas leaves and the surfactant solution were stirred and mixed evenly while hot to obtain 1000 ml of Jatropha curcas leaf composite disinfectant. The concentration of quaternary ammonium salt surfactant in the disinfectant was 0.11%.

[0055] The beneficial effects of the present application are demonstrated by the following specific experimental examples.

[0056] Experimental Example 1, Antibacterial effect of Jatropha curcas leaf composite disinfectant of the present application

[0057] 1. Grouping

[0058] Group A: Jatropha curcas leaf concentrate prepared according to the method described in Example 1, with 10% of Jatropha curcas leaf extract in the concentrate:

[0059] Group B: single-chain quaternary ammonium salt surfactant solution: single-chain quaternary ammonium salt surfactant benzalkonium chloride was diluted with water to obtain single-chain quaternary ammonium salt surfactant solutions with different concentrations: B1 (0.08%), B2 (0.09%), B3 (0.10%), and B4 (0.11%).

[0060] Group C: Jatropha curcas leaf composite disinfectant according to the present application: prepared according to the methods described in Examples 1-4, and divided into C1 (0.08%), C2 (0.09%), C3 (0.10%), and C4 (0.11%) according to the concentration of single-chain quaternary ammonium salt surfactant in the disinfectant.

[0061] 2. Method

[0062] 2.1 Inhibition of Staphylococcus aureus

[0063] (1) Test basis and method: Refer to the Bacterial Quantitative Killing Test Method in the Disinfection Technical Specifications, Ministry of Health (2002 edition) 2.1.1.7.

[0064] (2) Test conditions: A total of 9 samples of the three groups of disinfectants A, B, and C were used for the test, the test temperature was 20°C ± 1°C, and the test was repeated 3 times.

[0065] 2.2 Inhibition of Escherichia coli

[0066] (1) Test basis and method: Refer to the Bacterial Quantitative Killing Test Method in the Disinfection Technical Specifications, Ministry of Health (2002 edition) 2.1.1.7.

[0067] (2) Test conditions: A total of 9 samples of the three groups of disinfectants A, B, and C were used for the test, the test temperature was 20°C ± 1°C, and the test was repeated 3 times.

[0068] 3. Results

[0069] Figure 1 and Figure 3 The inhibition of Staphylococcus aureus by the three groups of disinfectants, a total of 9 samples, at 1 min, 5 min, 10 min, and 15 min, Figure 2 and Figure 4 The inhibition of Escherichia coli by the three groups of disinfectants, a total of 9 samples, at 1 min, 5 min, 10 min, and 15 min.

[0070] From Figures 1-4It can be seen that the inhibitory effect of the Jatropha curcas leaf concentrated solution of group A on Staphylococcus aureus and Escherichia coli is weak; the inhibitory effect of the single-chain quaternary ammonium salt surfactant of group B on Staphylococcus aureus and Escherichia coli is stronger than that of group A and is in a dose-dependent manner; the inhibitory effect of the Jatropha curcas leaf composite disinfectant of group C on Staphylococcus aureus and Escherichia coli is obviously better than that of group A and group B, and the killing logarithmic value of the Jatropha curcas leaf composite disinfectant of group C on various bacteria is the highest; through the SPSSAU software, it is known that the inhibitory effect of group C on Staphylococcus aureus and Escherichia coli is significantly different from that of group A and group B.

[0071] It is shown that the Jatropha curcas leaf composite disinfectant has the optimal antibacterial effect, compared with the single Jatropha curcas leaf concentrated solution and the single-chain quaternary ammonium salt surfactant, the Jatropha curcas leaf composite disinfectant plays a synergistic antibacterial role, and the Jatropha curcas leaf composite disinfectant has the broad-spectrum antibacterial effect and excellent antibacterial effect on gram-positive bacteria and gram-negative bacteria.

[0072] Experimental example 2, neutralizer identification test of the Jatropha curcas leaf composite disinfectant of the application

[0073] 1. Method

[0074] (1) Test basis: according to the neutralizer suspension quantitative identification test method in 2.1.1.5.5 of the Disinfection Technical Specification, Ministry of Health (2002 edition).

[0075] (2) Test conditions: the Jatropha curcas leaf composite disinfectant (0.08% benzalkonium chloride) prepared in example 1 is used to carry out the neutralizer identification test, the bactericidal effect is 2.5 min, the test temperature is 20℃±1℃, and the test is repeated for 3 times.

[0076] The experimental grouping is as follows:

[0077] Group 1: disinfectant + bacterial suspension

[0078] Group 2: (disinfectant + bacterial suspension) + neutralizer

[0079] Group 3: neutralizer + bacterial suspension

[0080] Group 4: (disinfectant + neutralizer) + bacterial suspension

[0081] Group 5: diluent (0.03 mol / L pH 7.2 PBS) + bacterial suspension

[0082] Group 6: diluent (0.03 mol / L pH 7.2 PBS) + neutralizer + culture medium, as a negative control

[0083] 2. Results

[0084] The results are shown in Table 1, the average number of bacteria of group 1 is 0 cfu / ml, the average number of bacteria of group 2 is 1.06x10 3The number of colonies of the bacteria grown in the second group was more than that in the first group, but was obviously less than that in the third, fourth and fifth groups; the number of colonies of the bacteria grown in the third, fourth and fifth groups was similar, and the error rates of the three groups were 7.31%, 5.83% and 6.78% respectively, which were less than 15%. The sixth group was a negative control, and no bacteria grew.

[0085] The neutralizing agent (2% Tween-80, 2% lecithin, 0.03 mol / L pH 7.2 PBS) used in the experiment can completely neutralize the disinfectant, and the neutralizing agent and the neutralization product have no adverse effects on Staphylococcus aureus. It meets the requirements of 2.1.1.5 of the Disinfection Technical Standard, Ministry of Health (2002 edition).

[0086] Table 1. Identification results of the neutralizing agent for the Jatropha curcas leaf composite disinfectant for killing Staphylococcus aureus

[0087]

[0088] Experimental Example 3, antibacterial effect of the Jatropha curcas leaf composite disinfectant of the application

[0089] 1. Method

[0090] (1) Test basis: The sterilization effect on Staphylococcus aureus and Escherichia coli was detected according to the bacterial quantitative killing test method of 2.1.1.7 of the Disinfection Technical Standard, Ministry of Health (2002 edition).

[0091] (2) Test conditions: The Jatropha curcas leaf composite disinfectant prepared in Example 1 (the concentration of benzalkonium chloride is 0.08%) was used for the test, the test temperature was 20℃±1℃, and the test was repeated three times.

[0092] 2. Results

[0093] Table 2 is the test results of the Jatropha curcas leaf composite disinfectant of the application for killing Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 8099). As shown in Table 2, the killing logarithm values of the Jatropha curcas leaf composite disinfectant (the concentration of benzalkonium chloride is 0.08%) for killing Staphylococcus aureus and Escherichia coli are all greater than 5.00 at 5 minutes, 10 minutes and 15 minutes, which meets the requirements of 2.1.1.7 of the Disinfection Technical Standard, Ministry of Health (2002 edition).

[0094] Table 2. Results of the Jatropha curcas leaf composite disinfectant for killing Staphylococcus aureus and Escherichia coli

[0095]

[0096] Note: The numbers in the brackets in the table are the maximum value and the minimum value in the three tests, and the negative control is sterile growth.

[0097] Experimental Example 4: Field Test Results of the Tabletop Disinfection Effect of the Jatropha curcas Leaf Compound Disinfectant of the Present Invention

[0098] 1. Method

[0099] (1) Test basis: Disinfection Technical Specifications (2002 edition) 2.1.2.10 Disinfectant identification test on other surfaces.

[0100] (2) Test method: On a wooden tabletop, two standard boards were used to mark two areas, each 25 cm². 2 The sample was divided into two sections: one for pre-disinfection sampling and one for post-disinfection sampling, with 30 samples tested in each section. Before disinfection, a sterile cotton swab was moistened with 5 ml of diluent (0.1% Tween PBS solution) and smeared on one area, repeating 8 times horizontally and 8 times vertically. The sampling end of the cotton swab was aseptically cut into the original diluent sample solution, and viable bacteria were counted after 10 minutes, serving as the control group. After disinfection, a 5 ml test tube containing neutralizing agent was used for sampling, and the same batch of diluent was used to inoculate the culture medium, serving as the experimental group. The inoculated petri dishes were incubated at 37°C for 48 hours, and the results were observed and the kill log value was calculated. The samples were then sterilized using the Jatropha curcas leaf compound disinfectant (0.08% benzalkonium chloride) prepared in Example 1 of this invention.

[0101] 2. Results

[0102] Table 3 shows the results of the field test of the jatropha leaf compound disinfectant (0.08% benzalkonium chloride) for desktop disinfection. As shown in Table 3, the average log reduction value of the jatropha leaf compound disinfectant against naturally occurring bacteria on the desktop after 10 minutes of contact is greater than 1.00, which indicates that the disinfection is qualified. This meets the requirements of Section 2.1.2.10 of the Ministry of Health's "Disinfection Technical Specifications" (2002 edition).

[0103] Table 3. Field test results of tabletop disinfection using Jatropha curcas leaf compound disinfectant (0.08% benzalkonium chloride).

[0104]

[0105]

[0106] Note: No bacterial growth was observed in the negative control group.

[0107] In conclusion, the jatropha leaf composite disinfectant prepared by using the jatropha leaf extract and the single-chain quaternary ammonium salt surfactant composition has broad-spectrum antibacterial effect, and the antibacterial effect is excellent, and compared with the single jatropha leaf extract and the single-chain quaternary ammonium salt surfactant, the synergistic antibacterial effect is exerted. Moreover, the jatropha leaf composite disinfectant is mainly derived from natural plants, the content of the single-chain quaternary ammonium salt surfactant is extremely small, the safety to human body is high when used, and the environmental pollution is extremely small. In addition, the jatropha leaf composite disinfectant utilizes the jatropha leaf extraction, the production process is simple, there is no any pollutant, the utilization rate of the jatropha leaf is improved, and the jatropha leaf composite disinfectant is green and environment-friendly, the obtained product is stable, and as an antibacterial disinfectant product, the jatropha leaf composite disinfectant has a good application prospect.

Claims

1. A composition with antibacterial and disinfecting properties, characterized in that: It is obtained by mixing the extract of Jatropha curcas leaves obtained by water extraction with an aqueous solution of a single-chain quaternary ammonium salt surfactant; in the mixture obtained by mixing, the concentration of Jatropha curcas leaf crude drug is 1%, and the concentration of the single-chain quaternary ammonium salt surfactant is 0.08~0.11%; the single-chain quaternary ammonium salt surfactant is benzalkonium chloride.

2. The composition according to claim 1, characterized in that: The preparation method of the Jatropha curcas leaf extract includes the following steps: take Jatropha curcas leaves, add water and boil to extract, filter, concentrate to obtain Jatropha curcas leaf extract.

3. The composition according to claim 2, characterized in that: The leaves of the small jatropha cotyledon are crushed and sieved before extraction; And / or, the weight of the added water is 20 to 35 times the weight of the jatropha leaves; And / or, the extraction time is 30-60 minutes; And / or, the concentration of jatropha leaf crude drug in the jatropha leaf extract is 10%.

4. A method for preparing the composition according to any one of claims 1 to 3, characterized in that: It includes the following steps: (1) Take the leaves of the small jatropha, boil them in water to extract them, filter them, concentrate them, and obtain the small jatropha leaf extract; (2) Dissolve the single-chain quaternary ammonium salt surfactant in water to obtain a single-chain quaternary ammonium salt surfactant solution; (3) Mix the extract of Jatropha curcas leaf with a single-chain quaternary ammonium salt surfactant solution to obtain the product.

5. Use of the composition according to any one of claims 1 to 3 in the preparation of an antibacterial agent; The antibacterial agent is effective against Staphylococcus aureus and / or Escherichia coli.

6. The use according to claim 5, characterized in that: The antibacterial agent is an antibacterial disinfectant.

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