A deep-sea multifunctional dual-cavity sequential sampler and its working method

Abstract

The present invention provides a deep-sea multifunctional dual-chamber sequential sampler and its working method. The multifunctional dual-chamber sequential sampler is composed of multiple dual-chamber sampling bottles and a power supply and control base. The dual-chamber sampling bottle consists of two parts: a heat-insulating biological enrichment chamber and an airtight non-pressure-maintaining water sampling chamber. Through the linear reciprocating electric cylinder hydraulic power unit at the bottom of the biological enrichment chamber and the vertical compressible oil sac connecting the two chambers, the enrichment sampling of microorganisms, the pressure maintenance in the water sampling chamber, and the extraction of metal ions are achieved. The power supply and control base consists of two layers, the upper layer provides power and control interfaces for docking with each water sampling bottle and fixing holes for the water sampling bottles, and the lower layer is the power supply and electronic controller compartment. The dual-chamber water sampling bottles are fixed to the power supply and control base at equal arc intervals. The electronic controller in the base is started in sequence according to the set time sequence and performs the sampling action.

Description

Technical Field

[0001] The present invention relates to the field of seabed sampling technology, and in particular to a deep-sea multifunctional dual-cavity sequential sampler and a working method thereof. Background Art

[0002] The extreme low temperature and high pressure environment of the deep sea creates its unique biological resources. Previous studies have shown that deep-sea microorganisms have extremely high abundance, activity, composition and functional diversity. They have formed special species, gene types and metabolites in the process of natural evolution, which are of great scientific and economic value. Carrying out analysis of the microbial, physical and chemical quantities of deep-sea water samples is one of the main tasks of marine resource exploration. The harsh environment of low temperature and high pressure in the deep sea poses a great challenge to the in-situ fidelity collection of water samples. If the pressure of the sample changes, the volatile and semi-volatile gases dissolved in the water sample will become supersaturated and overflow, causing the extracted sample to be distorted and unable to reflect the composition information of the seawater in situ.

[0003] Traditional deep-sea microbial sampling devices typically use simple open containers, lowered to a predetermined depth for sampling and recovery. This approach is limited by the volume of the sampling container, severely limiting the number of microorganisms that can be collected at a single time. Unidirectional deep-water pump-type microbial samplers using a power motor are limited by the biofilter's low pressure resistance and low enrichment factor, requiring large sampling bottles to collect large numbers of microorganisms. Furthermore, using a unidirectional pump for microbial enrichment prevents sequential multi-bottle sampling due to biofouling.

[0004] The main characteristics of my country's existing deep-sea pressure-maintaining water sampling equipment are weak pressure-maintaining performance and prone to leakage problems. Moreover, the existing relatively mature pressure-maintaining water samplers are not suitable for sea depths of 11,000 meters. The equipment can only be used once and cannot achieve multiple and large-scale collection, resulting in a waste of resources. Summary of the Invention

[0005] In order to make up for the deficiencies of the prior art, the present invention provides a deep-sea multifunctional dual-cavity sequential sampler and a working method thereof.

[0006] The present invention is achieved through the following technical solutions: a deep-sea multifunctional dual-cavity sequential sampler, comprising a power supply and controller base and 12 groups of sampling units mounted around the top of the base, wherein the sampling units are multifunctional dual-cavity sampling bottles, which are divided into inner and outer cavities, the inner side being a biological enrichment chamber and the outer side being an airtight water sampling chamber;

[0007] The top of the biological enrichment chamber is provided with a first electronic hydraulic valve and a second electronic hydraulic valve, wherein the first electronic hydraulic valve is a water inlet, the second electronic hydraulic valve is a water outlet, and the bottom of the second electronic hydraulic valve is provided with a barrier filter. A sampling filter group is provided in the biological enrichment chamber, and the sampling filter group is a plurality of filter units arranged in parallel up and down. The filter unit includes a biological retention table, and a conical structure made of mesh polyethylene material and a lightweight fluid one-way valve installed on the biological retention table. The bottom circular mouth of the conical structure is fixedly covered with a microbial filter membrane. The bottom of the biological enrichment chamber is a linear reciprocating electric cylinder hydraulic power unit, which is linearly reciprocating. The compound electric cylinder hydraulic power unit includes a flow-pushing plate, a compressible oil bag, a transmission rod and a linear drive motor. The linear drive motor is fixed to the bottom of the biological enrichment chamber by bolts. The linear drive motor is connected to the power supply and controller base through the power port and control port provided at the bottom of the bottle body. The bottom of the compressible oil bag is sealed and fixed to the bottom of the biological enrichment chamber. The transmission rod is installed through the interior of the compressible oil bag. The transmission rod is rigidly connected to the linear drive motor. The top end of the transmission rod is connected to the flow-pushing plate. The compressible oil bag is connected to the cabin where the linear drive motor is located. The oil body is connected to and fills the compressible oil bag and the cabin where the linear drive motor is located.

[0008] A third electronic hydraulic valve is provided at the top of the airtight water sampling chamber. A metal ion extraction platform is provided in the middle of the airtight water sampling chamber. The metal ion extraction platform has multiple built-in solid-phase extraction columns. The metal ion extraction platform and the airtight water sampling chamber body are rigidly and tightly connected. The bottom of the airtight water sampling chamber is an oil storage sac, and an electronic one-way valve is provided at the bottom of the oil storage sac. Nylon filters are installed on the tops of the first, second, and third electronic hydraulic valves. The bioaccumulation chamber and the airtight water sampling chamber are connected by a pressure-compensating passage between the compressible oil sac at the bottom and the oil storage sac.

[0009] The power supply and controller base is divided into two layers, including an upper base layer and a lower base layer. The upper base layer provides a fixing device and a cable interface, and the lower base layer is a power supply and controller compartment. The upper base layer is provided with a plurality of evenly distributed fixing holes, and the bottoms of the fixing holes are provided with a power interface and a control interface. The inner center of the upper base layer is provided with a power transmission port and a control port. The power interface and the control interface are respectively concentrated at the power transmission port and the control port through a cable trough provided at the bottom thereof. The transmission port is connected to the power supply installed in the lower base layer through a power supply line, and the control port is connected to the electronic controller installed in the lower base layer through a communication cable. The electronic controller and the power supply are connected through a power supply line.

[0010] The multifunctional dual-cavity sampling bottle is placed in the fixing hole, and the power port and control port of the bottle body are connected to the power interface and control interface respectively;

[0011] As a preferred solution, the mesh number per unit area of ​​the barrier filter is greater than or equal to the minimum mesh number per unit area of ​​the cone structure.

[0012] As a preferred solution, the energy sources of the first electronic hydraulic valve, the second electronic hydraulic valve and the third electronic hydraulic valve are all built-in power supplies.

[0013] As a preferred solution, the bottle wall of the biological enrichment chamber is a double-layer bottle body with inner and outer layers, and the space between the bottles is filled with insulation material.

[0014] A method for operating a deep-sea multifunctional dual-cavity sequential sampler comprises the following steps:

[0015] S1. When on shore or on deck, fill the bio-enrichment chamber and the airtight water sampling chamber of each sampling unit with deionized water, completely close the first electronic hydraulic valve and the third electronic hydraulic valve, and half-close the second electronic hydraulic valve.

[0016] S2. Set the start time of each sampling bottle in the controller. When the lander or other tow cable carrying the sampler reaches the specified height and the corresponding time is reached, the corresponding sampling units start to work in sequence;

[0017] S3: Start the preparatory action, open the third electronic hydraulic valve, control the linear drive motor to repeatedly push the flow plate up and down, and discharge the deionized water in the bio-enrichment chamber and the airtight water collection chamber;

[0018] S4: Start the working action, keep the third electronic hydraulic valve normally open, close the second electronic hydraulic valve, open the first electronic hydraulic valve, and the linear drive motor drives the flow plate downward to draw the ambient water into the bio-enrichment chamber and intercept microorganisms and suspended sediments on the filter unit, completing the suction action;

[0019] S5. After the suction action is completed, the first electronic hydraulic valve is closed, the second electronic hydraulic valve is opened, and the flow-pushing plate is pushed upward. The lightweight mechanical one-way valve is opened under the push of the upward water flow, allowing the filtered water below the bottom filter unit to be quickly discharged upward. The barrier filter at the bottom of the second electronic hydraulic valve will intercept the suspended organisms on the filter group caused by the upward flow process, completing the discharge action;

[0020] S6. Repeat steps S4-S5 several times to complete the enrichment action in the bio-enrichment chamber;

[0021] S7. During steps S4-S5, due to the pressure conduction of the oil bag between the two chambers, the oil bag in the airtight water sampling chamber moves in the opposite direction, driving the flow-pushing device to form repeated flushing of the solid phase extraction column;

[0022] S8, completely closing the first electronic hydraulic valve, the second electronic hydraulic valve, and the third electronic hydraulic valve, and opening the electronic one-way valve to maintain the same pressure inside and outside the sampling bottle during the recovery process through oil pressure buffering;

[0023] S9, complete the whole process of sampling with a single multifunctional double-chamber sampling bottle;

[0024] S10 and other multifunctional double-chamber sampling bottles complete their work in sequence according to the set time.

[0025] Due to the adoption of the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0026] 1. Utilizing a DC reciprocating pump, two controllable electronic valves (one with an isolation membrane), a sloped filter assembly, and a sample collection station, this system achieves microbial enrichment and concentration within a single, small-volume sampling unit. Compared to traditional one-way filtration, this method reduces the likelihood of filter assembly clogging and further improves microbial concentration.

[0027] 2. The pressure compensation chamber required by the reciprocating pump is converted into a pressure-maintaining oil bag of the water sampling unit through the pressure compensation passage between the cavities, so that the power of the biological enrichment chamber and the water sampling chamber can be shared, the pressure maintenance of the water sampling chamber can be controlled, and the number of structures required to achieve the same function can be reduced.

[0028] Additional aspects and advantages of the invention will become apparent from the description which follows, or may be learned by practice of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the following description of the embodiments with reference to the accompanying drawings, in which:

[0030] Figure 1 It is a structural schematic diagram of the present invention;

[0031] Figure 2 This is a perspective view of the structure of a multifunctional double-cavity sampling bottle;

[0032] Figure 3 It is a structural diagram of the power supply and controller base;

[0033] Figure 4 This is the connection diagram between the power supply and the controller base functional unit.

[0034] in, Figures 1 to 4 The corresponding relationship between the reference numerals and components is as follows:

[0035] 1 Bioaccumulation chamber, 2 Airtight water collection chamber on the outside, 3 First electronic hydraulic valve, 4 Second electronic hydraulic valve, 5 Third electronic hydraulic valve, 6 Barrier filter, 7 Conical structure, 8 Light fluid one-way valve, 9 Bioretention platform, 10 Insulation material, 11 Push flow plate, 12 Compressible oil sac, 13 Transmission rod, 14 Linear drive motor, 15 Power port, 16 Control port, 17 Nylon filter, 18 Solid phase extraction column, 19 Metal ion extraction platform, 20 Pressure replenishment passage, 21 Oil storage sac, 22 Electronic one-way valve, 23 Power supply and controller base, 24 Upper base layer, 25 Lower base layer, 26 Fixing hole, 27 Power interface, 28 Control interface, 29 Cable trough, 30 Control port, 31 Power transmission port, 32 Communication cable, 33 Power supply line, 34 Electronic controller, 35 Power supply. DETAILED DESCRIPTION

[0036] In order to more clearly understand the above-mentioned objects, features and advantages of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, in the absence of conflict, the embodiments of the present application and the features therein can be combined with each other.

[0037] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the scope of protection of the present invention is not limited to the specific embodiments disclosed below.

[0038] The following combination Figures 1 to 4 The deep-sea multifunctional dual-cavity sequential sampler and its working method according to the embodiment of the present invention are described in detail.

[0039] The purpose of the present invention is to solve the above-mentioned problems existing in the prior art, and proposes a multifunctional dual-chamber sequential sampler that combines in-situ microbial enrichment sampling and airtight non-pressure-maintaining water sampling. This multifunctional dual-chamber sequential sampler consists of a plurality of dual-chamber sampling bottles and a power supply and control base; wherein the dual-chamber sampling bottle consists of two parts: a heat-insulating biological enrichment chamber and an airtight non-pressure-maintaining water sampling chamber. Through the linear reciprocating electric cylinder hydraulic power unit at the bottom of the biological enrichment chamber and the vertical compressible oil bag connecting the two chambers, the enrichment sampling of microorganisms and the pressure maintenance in the water sampling chamber and the extraction of metal ions are realized. The power supply and control base consists of two layers, the upper layer provides the power supply and control interface for docking with each water sampling bottle and the fixing hole of the water sampling bottle, and the lower layer is the power supply and electronic controller compartment. The dual-chamber water sampling bottles are fixed with the power supply and control base at equal arc intervals, and the electronic controller in the base is started in sequence according to the set time sequence and performs the sampling action.

[0040] The deep-sea multifunctional dual-chamber time-series sampler, with a smaller volume and fewer structures, can complete the pressure-maintained collection of deep-sea in-situ seawater, the controllable enrichment and collection of in-situ microorganisms, and the extraction of trace metal elements, providing a new solution for studying the biological, chemical, and physical information of the deep-sea environment.

[0041] like Figure 1 As shown, the present invention proposes a deep-sea multifunctional dual-cavity sequential sampler, comprising a power supply and controller base 23 and 12 sets of sampling units mounted around the top thereof. The sampling units are multifunctional dual-cavity sampling bottles, which are divided into two cavities, the inner side being a biological enrichment chamber 1 and the outer side being an airtight water sampling chamber 2.

[0042] like Figure 2 As shown, the top of the bioaccumulation chamber 1 is equipped with two independent electronic hydraulic valves, namely a first electronic hydraulic valve 3 and a second electronic hydraulic valve 4. The bottle wall of the bioaccumulation chamber 1 is a double-layer bottle with an inner and outer layer, and the space between the bottles is filled with insulation material 10. The first electronic hydraulic valve 3 is the water inlet, and the second electronic hydraulic valve 4 is the water outlet. The bottom of the second electronic hydraulic valve 4 is equipped with a barrier filter 6. The mesh size per unit area of ​​the barrier filter 6 is greater than or equal to the minimum mesh size per unit area of ​​the conical structure 7. A sampling filter group is provided in the biological enrichment chamber 1. The sampling filter group is a plurality of filter units arranged in parallel up and down. The filter unit includes a biological retention table 9, and a conical structure 7 made of a mesh polyethylene material and a light fluid one-way valve 8 installed on the biological retention table 9. The mesh number per unit area of ​​the conical structure 7 can be set in a gradient according to actual needs. The bottom circular opening of the conical structure 7 is fixedly covered with a microbial filter membrane. The bottom of the biological enrichment chamber 1 is a linear reciprocating electric cylinder hydraulic power unit, which includes a flow pusher 11, a compressible oil bag 12, a transmission rod 13 and a linear drive motor 14. The linear drive motor 14 is fixed to the bottom of the biological enrichment chamber 1 by bolts. The linear drive motor 14 is connected to the power supply and controller base 23 through the power port 15 and the control port 16 provided at the bottom of the bottle body. The bottom of the compressible oil sac 12 is sealed and fixed to the bottom of the biological enrichment chamber 1. The transmission rod 13 is installed through the interior of the compressible oil sac 12. The transmission rod 13 and the linear drive motor 14 are rigidly connected. The top of the transmission rod 13 is connected to the flow-pushing plate 11. The compressible oil sac 12 and the cabin where the linear drive motor 14 is located are connected, and the oil body is connected to and fills the cabin where the compressible oil sac 12 and the linear drive motor 14 are located.

[0043] A third electronic hydraulic valve 5 is provided at the top of the airtight water sampling chamber 2. A metal ion extraction platform 19 is provided in the middle of the airtight water sampling chamber 2. The metal ion extraction platform 19 houses multiple solid-phase extraction columns 18. The metal ion extraction platform 19 is rigidly and tightly connected to the airtight water sampling chamber 2. An oil reservoir 21 is located at the bottom of the airtight water sampling chamber 2. An electronic one-way valve 22 is provided at the bottom of the oil reservoir 21 to maintain the same pressure inside and outside the sampling bottle during the recovery process through oil pressure buffering. Nylon filters 17 are installed at the tops of the first, second, and third electronic hydraulic valves 3, 4, and 5. The energy sources for the first, second, and third electronic hydraulic valves 3, 4, and 5 are all built-in power supplies. The bioaccumulation chamber 1 and the airtight water sampling chamber 2 are connected via a pressure-compensating passage 20 between the compressible oil sac 12 at the bottom and the oil reservoir 21.

[0044] like Figure 3 、 4 As shown, the power supply and controller base 23 includes two layers, including an upper base layer 24 and a lower base layer 25. The upper base layer 24 provides a fixing device and a cable interface, and the lower base layer 25 is a power supply and controller compartment. The upper base layer 24 is provided with a plurality of evenly distributed fixing holes 26. The bottom of the fixing holes 26 is provided with a power interface 27 and a control interface 28. The inner center of the upper base layer 24 is provided with a power transmission port 31 and a control port 30. The power interface 27 and the control interface 28 are respectively concentrated at the power transmission port 31 and the control port 30 through a cable groove 29 provided at the bottom thereof. The power transmission port 31 is connected to a power supply 35 installed in the lower base layer 25 through a power supply line 33. The control port 30 is connected to an electronic controller 34 installed in the lower base layer 25 through a communication cable 32. The electronic controller 34 and the power supply 35 are connected through a power supply line 33.

[0045] The multifunctional dual-cavity sampling bottle is placed in the fixing hole 26, and the power port 15 and the control port 16 of the bottle body are connected to the power interface 27 and the control interface 28 respectively, realizing the shared integration of power supply and control. By arranging multiple dual-cavity sampling bottles in equal arcs on the base, the time-sequential water sampling function of the entire device is realized;

[0046] A method for operating a deep-sea multifunctional dual-cavity sequential sampler comprises the following steps:

[0047] S1. When on shore or on deck, fill the bio-enrichment chamber 1 and the airtight water sampling chamber 2 of each sampling unit with deionized water, completely close the first electronic hydraulic valve 3 and the third electronic hydraulic valve 5, and half-close the second electronic hydraulic valve 4.

[0048] S2. Set the start time of each sampling bottle in the controller. When the lander or other tow cable carrying the sampler reaches the specified height and the corresponding time is reached, the corresponding sampling units start to work in sequence;

[0049] S3, start the preparatory action, open the third electronic hydraulic valve 5, control the linear drive motor 14 to repeatedly push the flow-pushing plate 11 up and down, and discharge the deionized water in the biological enrichment chamber 1 and the airtight water collection chamber 2;

[0050] S4: Start the working action, keep the third electronic hydraulic valve 5 normally open, close the second electronic hydraulic valve 4, open the first electronic hydraulic valve 3, and the linear drive motor 14 drives the flow-pushing plate 11 downward to suck the ambient water into the bio-enrichment chamber 1, and intercept the microorganisms and suspended sediments on the filter unit, completing the suction action;

[0051] S5: After the suction action is completed, the first electronic hydraulic valve 3 is closed, the second electronic hydraulic valve 4 is opened, and the flow-pushing plate 11 is pushed upward. The lightweight mechanical one-way valve 8 is opened under the push of the upward water flow, allowing the filtered water below the bottom filter unit to be quickly discharged upward. The barrier filter 6 at the bottom of the second electronic hydraulic valve 4 will intercept the biological suspension on the filter group caused by the upward flow process, completing the discharge action;

[0052] S6. Repeat steps S4-S5 several times to complete the enrichment operation in the bio-enrichment chamber 1;

[0053] S7. During steps S4-S5, due to the pressure conduction of the oil bag between the two chambers, the oil storage bag 21 in the airtight water sampling chamber 2 moves in the opposite direction, and drives the flow-pushing device to form repeated flushing of the solid phase extraction column 18, thereby improving the extraction efficiency;

[0054] S8, completely close the first electronic hydraulic valve 3, the second electronic hydraulic valve 4 and the third electronic hydraulic valve 5, open the electronic one-way valve 22, and maintain the same pressure inside and outside the sampling bottle during the recovery process through the buffering of oil pressure;

[0055] S9, complete the whole process of sampling with a single multifunctional double-chamber sampling bottle;

[0056] S10 and other multifunctional double-chamber sampling bottles complete their work in sequence according to the set time.

[0057] In the description of the present invention, the term "plurality" refers to two or more than two. Unless otherwise expressly defined, the orientations or positional relationships indicated by the terms "upper" and "lower" are based on the orientations or positional relationships shown in the accompanying drawings. They are intended only to facilitate the description of the present invention and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they should not be understood as limitations on the present invention. The terms "connect," "install," and "fix" should be understood in a broad sense. For example, "connection" can mean a fixed connection, a detachable connection, or an integral connection; it can be a direct connection or an indirect connection through an intermediate medium. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0058] Throughout this specification, terms such as "one embodiment," "some embodiments," and "specific embodiments" mean that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0059] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A deep-sea multifunctional dual-chamber sequential sampler, comprising a power supply and controller base (23) and a plurality of sampling units mounted around the top thereof, characterized in that The sampling unit is a multifunctional double-cavity sampling bottle, which is divided into two cavities, the inner cavity is a biological enrichment cavity (1), and the outer cavity is an airtight water sampling cavity (2); The top of the biological enrichment chamber (1) is provided with a first electronic hydraulic valve (3) and a second electronic hydraulic valve (4), wherein the first electronic hydraulic valve (3) is a water inlet, the second electronic hydraulic valve (4) is a water outlet, and the bottom of the second electronic hydraulic valve (4) is provided with a barrier filter (6). A sampling filter group is provided in the cavity of the biological enrichment chamber (1), and the sampling filter group is a plurality of filter units arranged in parallel up and down. The filter unit includes a biological retention table (9), and a conical structure (7) made of a mesh polyethylene material and a light fluid one-way valve (8) installed on the biological retention table (9). The bottom circular opening of the conical structure (7) is fixedly covered with a microbial filter membrane. The bottom of the biological enrichment chamber (1) is a linear reciprocating electric cylinder hydraulic power unit, and the linear reciprocating electric cylinder hydraulic power unit includes a flow push plate (11), a reciprocating A compression oil sac (12), a transmission rod (13) and a linear drive motor (14), wherein the linear drive motor (14) is fixed to the bottom of the biological enrichment chamber (1) by bolts, and the linear drive motor (14) is connected to the power supply and controller base (23) through the power port (15) and the control port (16) provided at the bottom of the bottle body, and the bottom of the compressible oil sac (12) is sealed and fixed to the bottom of the biological enrichment chamber (1), and the transmission rod (13) is installed through the inside of the compressible oil sac (12), and the transmission rod (13) and the linear drive motor (14) are rigidly connected, and the top end of the transmission rod (13) is connected to the push plate (11), and the compressible oil sac (12) and the cabin where the linear drive motor (14) is located are connected, and the oil body is connected to and fills the cabin where the compressible oil sac (12) and the linear drive motor (14) are located; The top of the airtight water sampling chamber (2) is provided with a third electronic hydraulic valve (5), a metal ion extraction platform (19) is provided in the middle of the airtight water sampling chamber (2), the metal ion extraction platform (19) has multiple solid phase extraction columns (18) built in the metal ion extraction platform (19), the metal ion extraction platform (19) and the airtight water sampling chamber (2) are rigidly and tightly connected, the bottom of the airtight water sampling chamber (2) is an oil storage bag (21), the bottom of the oil storage bag (21) is provided with an electronic one-way valve (22), the tops of the first electronic hydraulic valve (3), the second electronic hydraulic valve (4) and the third electronic hydraulic valve (5) are provided with a nylon filter (17), and the biological enrichment chamber (1) and the airtight water sampling chamber (2) are connected through a pressure compensation passage (20) between the compressible oil bag (12) at the bottom and the oil storage bag (21); The power supply and controller base (23) includes two layers, an upper base layer (24) and a lower base layer (25). The upper base layer (24) is provided with a fixing device and a cable interface, and the lower base layer (25) is a power supply and controller compartment. The upper base layer (24) is provided with a plurality of evenly distributed fixing holes (26). The bottom of the fixing holes (26) is provided with a power supply interface (27) and a control interface (28). The inner center of the upper base layer (24) is provided with a power transmission port (31) and a control port (30). The power interface (27) and the control interface (28) are respectively concentrated at the power transmission port (31) and the control port (30) through the cable trough (29) provided at the bottom thereof, wherein the power transmission port (31) is connected to the power supply (35) installed in the lower layer (25) of the base through the power supply line (33), and the control port (30) is connected to the electronic controller (34) installed in the lower layer (25) of the base through the communication cable (32), and the electronic controller (34) and the power supply (35) are connected through the power supply line (33); The multifunctional dual-cavity sampling bottle is placed in the fixing hole (26), and the power port (15) and the control port (16) of the bottle body are connected to the power interface (27) and the control interface (28) respectively.

2. A deep-sea multifunctional dual-cavity sequential sampler according to claim 1, characterized in that , 12 groups of multifunctional double-cavity sampling bottles are arranged on the top of the power supply and controller base (23).

3. A deep-sea multifunctional dual-cavity sequential sampler according to claim 1, characterized in that The mesh number per unit area of ​​the barrier filter (6) is greater than or equal to the minimum mesh number per unit area of ​​the cone structure (7).

4. A deep-sea multifunctional dual-cavity sequential sampler according to claim 1, characterized in that The energy sources of the first electronic hydraulic valve (3), the second electronic hydraulic valve (4) and the third electronic hydraulic valve (5) are all built-in power supplies.

5. A deep-sea multifunctional dual-cavity sequential sampler according to claim 1, characterized in that The bottle wall of the biological enrichment chamber (1) is a double-layer bottle body with inner and outer layers, and the space between the bottles is filled with insulation material (10).

6. A method for operating a deep-sea multifunctional dual-cavity sequential sampler, using the deep-sea multifunctional dual-cavity sequential sampler according to any one of claims 1 to 5, characterized in that , specifically including the following steps: S1. When on shore or on deck, fill the bio-enrichment chamber (1) and the airtight water sampling chamber (2) of each sampling unit with deionized water, completely close the first electronic hydraulic valve (3) and the third electronic hydraulic valve (5), and half-close the second electronic hydraulic valve (4); S2. Set the start time of each sampling bottle in the controller. When the lander or other tow cable carrying the sampler reaches the specified height and the corresponding time is reached, the corresponding sampling units start to work in sequence; S3, start the preparatory action, open the third electronic hydraulic valve (5), control the linear drive motor (14) to repeatedly push the flow-pushing plate (11) up and down, and discharge the deionized water in the biological enrichment chamber (1) and the airtight water collection chamber (2); S4, start the working action, keep the third electronic hydraulic valve (5) normally open, close the second electronic hydraulic valve (4), open the first electronic hydraulic valve (3), and the linear drive motor (14) drives the flow-pushing plate (11) downward to suck the environmental water into the biological enrichment chamber (1), and intercept the microorganisms and suspended sediments on the filter unit, completing the suction action; S5. After the suction action is completed, the first electronic hydraulic valve (3) is closed, the second electronic hydraulic valve (4) is opened, and the flow-pushing plate (11) is pushed upward. The light fluid one-way valve (8) is opened under the push of the upward water flow, so that the filtered water below the bottom filter unit can be quickly discharged upward. The barrier filter (6) at the bottom of the second electronic hydraulic valve (4) will intercept the biological suspension on the filter group caused by the upward flow process, completing the discharge action; S6. Repeat steps S4-S5 several times to complete the enrichment action in the bio-enrichment chamber (1); S7. During steps S4-S5, due to the pressure conduction of the oil bag between the two chambers, the oil storage bag (21) in the airtight water sampling chamber (2) moves in the opposite direction synchronously, and drives the flow-pushing device to form a repeated flushing flow to the solid phase extraction column (18); S8, completely closing the first electronic hydraulic valve (3), the second electronic hydraulic valve (4), and the third electronic hydraulic valve (5), opening the electronic one-way valve (22), and maintaining the same pressure inside and outside the sampling bottle during the recovery process through the buffering of oil pressure; S9, complete the whole process of sampling for a single sampling unit; S10. Other sampling units complete their work in sequence according to the set time.

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