Molecular diagnostic apparatus and test card test seat for a molecular diagnostic apparatus

Abstract

The application provides a molecular diagnostic device and a detection card detection seat for the molecular diagnostic device, and relates to the technical field of molecular detection. In the detection card detection seat, a light source is used for emitting excitation light; a first lens module is used for receiving and transmitting the excitation light; a functional membrane group is used for receiving and transmitting the excitation light transmitted by the first lens module; an excitation light fiber is used for transmitting the excitation light transmitted by the functional membrane group, and is used for irradiating the detection card with the excitation light; a receiving light fiber is used for receiving fluorescence generated by the detection card irradiated by the excitation light; a second lens module is used for receiving the fluorescence transmitted by the receiving light fiber; and a sensor is used for receiving the fluorescence transmitted by the second lens module. The application processes the excitation light by using the first lens module and the functional membrane group, processes the fluorescence by using the second lens module, and the overall structure is related to the light source and the light sensor, so that the overall structure is relatively simple, and is suitable for use in the molecular diagnostic device.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of molecular detection, in particular to a molecular diagnostic device and a detection card detection seat for the molecular diagnostic device. BACKGROUND

[0002] The molecular diagnostic device utilizes molecular diagnostic technology. The molecular diagnostic technology refers to a diagnostic technology using nucleic acids or proteins as biomarkers for clinical detection, which provides information and decision basis for prediction, diagnosis, prevention, treatment and outcome of diseases.

[0003] When the molecular diagnostic device detects the detection card, the light path for detecting the detection card is complex and cannot be adapted to the detection card. SUMMARY

[0004] One aspect of the present application provides a detection card detection seat for a molecular diagnostic device, comprising:

[0005] A light generator for generating excitation light, the light generator comprising:

[0006] A light source for emitting excitation light;

[0007] A first lens module for receiving and transmitting the excitation light; and

[0008] A functional film group for receiving and transmitting the excitation light transmitted through the first lens module;

[0009] An excitation light fiber for transmitting the excitation light transmitted through the functional film group, for irradiating the detection card with the excitation light;

[0010] A receiving light fiber for receiving fluorescence generated by the detection card irradiated by the excitation light; and

[0011] A light receiving assembly for receiving the fluorescence transmitted by the receiving light fiber, the light receiving assembly comprising:

[0012] A second lens module for receiving the fluorescence transmitted by the receiving light fiber; and

[0013] A light sensor for receiving the fluorescence transmitted through the second lens module.

[0014] In another aspect, the present application also provides a molecular diagnostic device, comprising:

[0015] A support seat for supporting a detection card, the detection card comprising a sample adding cavity and a detection cavity; and

[0016] A detection assembly for detecting the detection cavity, the detection assembly comprising:

[0017] a light source for emitting excitation light;

[0018] a first lens module for receiving and transmitting the excitation light;

[0019] a functional film set for receiving and transmitting the excitation light transmitted by the first lens module;

[0020] an excitation light fiber for transmitting the excitation light transmitted by the functional film set, one end of the excitation light fiber being arranged on the support seat to irradiate the detection cavity with the excitation light;

[0021] a receiving light fiber, one end of which is arranged on the support seat to receive fluorescent light generated by irradiating the detection cavity with the excitation light;

[0022] a second lens module for receiving the fluorescent light transmitted by the receiving light fiber; and

[0023] a light sensor for receiving the fluorescent light transmitted by the second lens module.

[0024] In another aspect, the present application also provides a molecular diagnostic device, comprising:

[0025] a light source for emitting excitation light;

[0026] a first lens module for receiving and transmitting the excitation light;

[0027] a functional film set for receiving and transmitting the excitation light transmitted by the first lens module;

[0028] an excitation light fiber for transmitting the excitation light transmitted by the functional film set, for irradiating a detection card with the excitation light;

[0029] a receiving light fiber for receiving fluorescent light generated by irradiating the detection card with the excitation light; and

[0030] a light receiving assembly for receiving the fluorescent light transmitted by the receiving light fiber, the light receiving assembly comprising:

[0031] an assembly housing provided with a filling hole penetrating through the assembly housing, one end of the receiving light fiber being arranged in the filling hole;

[0032] a light path assembly arranged in the filling hole for receiving the fluorescent light transmitted by the receiving light fiber;

[0033] a light receiving member fixed to one side of the assembly housing, the light receiving member being provided with a light sensor, the light sensor being arranged opposite to the filling hole to receive the fluorescent light transmitted by the light path assembly; and

[0034] A clamping piece is disposed on the side of the component housing away from the optical receiver. The clamping piece is fixedly connected to the component housing to secure the receiving optical fiber in the filling hole.

[0035] This application utilizes a first lens module and a functional membrane assembly to process the excitation light, and a second lens module to process the fluorescence. The overall structure also involves a light source and a light sensor, so the overall structure is relatively simple and suitable for use in molecular diagnostic equipment. Attached Figure Description

[0036] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the embodiments will be briefly described below. Obviously, the drawings described below are merely some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0037] Figure 1 This is a three-dimensional structural diagram of a molecular diagnostic device in one embodiment of this application;

[0038] Figure 2 for Figure 1 Exploded view of a molecular diagnostic device;

[0039] Figure 3 For this application Figure 2 A schematic diagram of the explosion of the pressure plate.

[0040] Figure 4 for Figure 2 A schematic diagram of the structure of the mid-cargo component;

[0041] Figure 5 for Figure 2 Exploded view of the detection card holder in the middle;

[0042] Figure 6 for Figure 5 Exploded view of the central support base;

[0043] Figure 7 and Figure 8 They are respectively Figure 6 Schematic diagrams of the main body of the central support from different perspectives;

[0044] Figure 9 for Figure 6 Schematic diagram of the structure of the Zhongguang detection component;

[0045] Figure 10 for Figure 9 A cross-sectional view of the XX section along the detection seat in the middle;

[0046] Figure 11 for Figure 6Structure diagram of the middle sample adding cavity assembly;

[0047] Figure 12 For Figure 11 Sectional view of the middle sample adding cavity assembly along line XII-XII;

[0048] Figure 13 For Figure 5 Structure diagram of the middle light generator;

[0049] Figure 14 For Figure 13 Sectional view of the first light generator;

[0050] Figure 15 For Figure 5 Exploded view of the light receiving assembly;

[0051] Figure 16 For Figure 5 Structure diagram of the light receiving member;

[0052] Figure 17 For Figure 15 Structure diagram of the middle sub-light path assembly;

[0053] Figure 18 For Figure 17 Sectional view of the middle sub-light path assembly;

[0054] Figure 19 For Figure 15 Structure diagram of the first gasket;

[0055] Figure 20 For Figure 15 Structure diagram of the second gasket;

[0056] Figure 21 For Figure 15 Structure diagram of the second clamping piece;

[0057] Figure 22 For Structure diagram of the detection card in an embodiment of the application;

[0058] Figure 23 Figure 22 For Sectional view of the detection card along line L-L;

[0059] Figure 24 Figure 22 For Structure diagram of the detection card in an embodiment of the application;

[0060] With reference to the drawings, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0061] Reference herein to“an embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase that the phrase in various places in the specification are not necessarily all referring to the same embodiment, or are necessarily referring to different or alternative embodiments. It is explicitly and implicitly understood that the embodiments described herein can be combined with other embodiments.

[0062] Next, a molecular diagnostic device is described, which utilizes a molecular diagnostic technology. The molecular diagnostic technology refers to a diagnostic technology that utilizes nucleic acids or proteins as biomarkers for clinical detection, and provides information and decision-making basis for prediction, diagnosis, prevention, treatment and outcome of diseases. In particular, in the face of various sudden infectious diseases, the most economical and effective measure is rapid and accurate molecular diagnosis.

[0063] Please refer to Figure 1 and Figure 2 , Figure 1 is a perspective view of a molecular diagnostic device 100 in an embodiment of the present application, Figure 2 is Figure 1 an exploded view of the molecular diagnostic device 100 in the embodiment. The molecular diagnostic device 100 can include a rack 10, a detection card conveying seat 20 mounted on the rack 10, a detection card detection seat 30 mounted on the rack 10, and a control circuit board 40 mounted on the rack 10. The detection card conveying seat 20 can be used to place a detection card. The detection card conveying seat 20 can slide on the rack 10 relative to the rack 10, so that the detection card conveying seat 20 can carry the detection card and carry it to the detection card detection seat 30. The detection card detection seat 30 is used to generate excitation light to detect the detection card and form a detection signal. The control circuit board 40 can be used to control the sliding of the detection card conveying seat 20 on the rack 10, and control the detection of the detection card by the detection card detection seat 30, and receive and process the detection signal to form diagnostic data.

[0064] It can be understood that, in some embodiments, the molecular diagnostic apparatus 100 can further comprise a cabinet. The cabinet can accommodate the rack 10, the test card conveying seat 20, the test card detecting seat 30, the control circuit board 40, etc. therein to protect the molecular diagnostic apparatus 100. The cabinet can also reduce the interference of external factors on the test card detecting process. In some embodiments, the cabinet can be provided with an isolation door, so that when the isolation door is opened, the test card conveying seat 20 can be slid out of the isolation door, thereby facilitating the placement and removal of the test card on the test card conveying seat 20. When the isolation door is closed, the protection effect of the cabinet and the interference reduction effect of external factors can be achieved.

[0065] In addition, in some embodiments, the molecular diagnostic apparatus 100 can further comprise output devices such as a display and a printer which can be electrically connected to the control circuit board 40, to output the diagnostic data of the molecular diagnostic apparatus 100 through the output devices. Of course, the molecular diagnostic apparatus 100 can also be provided with a memory to store the diagnostic data.

[0066] Furthermore, in some embodiments, the molecular diagnostic apparatus 100 can further comprise input devices such as a display, a keyboard, and a code scanning device which can be electrically connected to the control circuit board 40, to input control instructions to the molecular diagnostic apparatus 100 such as the control circuit board 40 through the input devices, so that the molecular diagnostic apparatus 100 can control the test card conveying seat 20 and / or the test card detecting seat 30 through the control circuit board 40.

[0067] Please refer to Figure 2 . The rack 10 can comprise a rack body 11 and a driving device 12 mounted on the rack body 11. The rack body 11 is used to mount structures such as the test card conveying seat 20, the test card detecting seat 30, and the control circuit board 40. The driving device 12 is used to be electrically connected to the control circuit board 40 to accept the control of the control circuit board 40. The driving device 12 is used to be connected to the test card conveying seat 20 to drive the test card conveying seat 20 to slide relative to the rack body 11 under the control of the control circuit board 40, thereby achieving the conveyance of the test card.

[0068] It should be noted that the terms "first", "second", etc. in the present text are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features.

[0069] The rack body 11 can be in a frame structure as a whole. The rack body 11 can be internally used for mounting the detection card conveying seat 20 and the detection card detection seat 30, and the detection card conveying seat 20 is located above the detection card detection seat 30. Of course, in some embodiments, the installation positions of the detection card conveying seat 20 and the detection card detection seat 30 on the rack body 11 can also be other, and details are not described herein.

[0070] In this article, "up", "down", "front", "back", "left", "right", "top", "bottom", "upper", "lower", "center", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like are used to describe the orientation or positional relationship. The terms "center", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only used for the convenience of describing the present application and simplifying the description. Therefore, it cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0071] Referring to Figure 2 , the detection card conveying seat 20 can include a pressing disc 50 and a carrying assembly 60 mounted on the rack 10, for example, the rack body 11. The carrying assembly 60 is used to place the detection card. The pressing disc 50 is connected with the driving device 12, so as to slide on the rack body 11 under the action of the driving device 12, thereby driving the carrying assembly 60 to slide on the rack body 11. The carrying assembly 60 can carry the detection card and carry it to the detection card detection seat 30.

[0072] Referring to Figure 3 , Figure 3 The present application Figure 2An exploded view of the pressing disc 50. The pressing disc 50 can include a first housing 51 mounted on the frame 10, e.g., the frame body 11, an electromagnetic member 52 disposed on a side of the first housing 51 facing the detection card detection seat 30, a pressing member 53 for fixing a detection card on the shipping assembly 60, a heating assembly 54 disposed on the first housing 51, a first circuit board 55 mounted on the first housing 51 and electrically connected with the electromagnetic member 52 and the heating assembly 54 respectively, a locking member 56 mounted on the first housing 51 and used for fixing the pressing member 53, a second housing 57 covering a side of the first housing 51 away from the electromagnetic member 52, and a first slide rail assembly 58 disposed on the first housing 51 and mounted on the frame 10, e.g., the frame body 11. The first housing 51 and the second housing 57 are fastened to form a pressing disc body. The first circuit board 55 can be electrically connected with the control circuit board 40. The electromagnetic member 52 can generate a magnetic force under the control of the control circuit board 40 to attract the pressing member 53 to the first housing 51. The electromagnetic member 52 can eliminate the magnetic force under the control of the control circuit board 40 to avoid attracting the pressing member 53. The heating assembly 54 can heat the detection card under the control of the control circuit board 40. The first slide rail assembly 58 can slide on the frame 10, e.g., the frame body 11. The first housing 51 is connected with the frame 10, e.g., the frame body 11, to slide on the frame body 11.

[0073] Please refer to Figure 4 , Figure 4 for Figure 2 An exploded view of the shipping assembly 60. The shipping assembly 60 can include a slide frame 61 disposed above the pressing disc 50 and mounted on the frame 10, e.g., the frame body 11, and a shipping member 62 mounted on the slide frame 61. The slide frame 61 can slide on the frame 10, e.g., the frame body 11. The slide frame 61 is connected with the pressing disc 50 to slide on the frame 10 together with the pressing disc 50 in some scenarios. The shipping member 62 can be used to place a detection card. The shipping member 62 can slide relative to the slide frame 61. The sliding direction of the shipping member 62 relative to the slide frame 61 is different from the sliding direction of the slide frame 61 relative to the frame 10.

[0074] It can be understood that when the shipping assembly 60 is extended, the shipping member 62 slides on the slide frame 61 to slide to a first position outside the frame 10, to complete the extension, and to place a detection card when the shipping assembly 60 is in the extended state. Then, when the shipping assembly 60 is retracted, the shipping member 62 slides on the slide frame 61 to slide to a second position inside the frame 10, to complete the retraction, and the shipping assembly 60 can slide between a third position and a fourth position on the frame body 11 when the shipping assembly 60 is in the retracted state. In some embodiments, the shipping assembly 60 can be extended at the third position. In some embodiments, the shipping assembly 60 can be extended at a position between the third position and the fourth position.

[0075] Please refer to Figure 5 , Figure 5 To Figure 2 the exploded view of the detection card detection seat 30. The detection card detection seat 30 can include a support frame 70 fixedly connected with the rack 10, a support seat 80 mounted on the support frame 70, and a detection assembly 90 mounted on the support frame 70 and the support seat 80. Among them, the support seat 80 is located below the pressing disc 50, so as to carry the detection card conveying seat 20 conveyed by the conveying assembly 60, for example. The detection assembly 90 is electrically connected with the control circuit board 40. When the detection assembly 90 places the detection card on the support seat 80, a series of detection processes are performed on the detection card by using excitation light to generate detection signals, and the detection signals are transmitted to the control circuit board 40.

[0076] Please refer to Figure 5 , the support frame 70 is in a frame structure as a whole, and can be located at the bottom of the rack 10. The support frame 70 can include a base 71 arranged at the bottom of the rack 10 and fixedly connected with the rack 10, and a fixed seat 72 mounted on the top of the base 71.

[0077] The base 71 can include a base body 711. The base body 711 can be made of a hard material such as plastic, metal, etc. The base body 711 can be in a plate structure as a whole, and of course can also be in other structures, which will not be described herein. The base 71 can be fixedly connected with the rack 10, for example, the rack body 11 by means of insertion, screwing, welding, bonding, buckling, etc.

[0078] The top of the base body 711 can be provided with a fixed groove 712 in the middle, for accommodating and fixing the fixed seat 72. In an embodiment, the fixed groove 712 can be omitted, and the fixed seat 72 can be directly fixed on the base 71.

[0079] The base body 711 is provided with a plurality of isolation grooves 713 around the circumference of the fixed groove 712, for cooperating with the detection assembly 90. The fixed part 714 is formed between two adjacent isolation grooves 713, for cooperating with the detection assembly 90. The plurality of isolation grooves 713 can reduce the contact area between the detection assembly 90 and the base body 711. In an embodiment, the isolation grooves 713 can be omitted.

[0080] The top of the base body 711 is provided with a support part 715 for supporting the support seat 80. The support part 715 can be fixedly connected with the base body 711 by means of insertion, screwing, welding, bonding, buckling, etc. In an embodiment, the support part 715 can be in a rod structure, and of course the support part 715 can also be in other structures. In an embodiment, the support part 715 can be omitted in the case that other structures support the support seat 80.

[0081] The bottom body 711 is provided with a positioning light receiver 716 opposite to the positioning light generator at the top, so as to receive the light emitted by the positioning light generator, and then realize the positioning of the detection card. The positioning light receiver 716 can be electrically connected with the control circuit board 40, so that after the positioning light receiver 716 receives the light, a signal is generated and transmitted to the control circuit board 40, so that the molecular diagnostic equipment 100 confirms that the detection card reaches the predetermined position. In some embodiments, the installation position of the positioning light generator and the installation position of the positioning light receiver 716 can be interchanged.

[0082] The fixing seat 72 can include a mounting seat 721 placed in the fixing groove 712. The mounting seat 721 can be made of a hard material such as plastic, metal, etc. The mounting seat 721 can be fixed on the bottom body 711 by means of insertion, screwing, welding, bonding, buckling, etc. The mounting seat 721 can be used to mount the detection assembly 90.

[0083] The top of the mounting seat 721 can be provided with a first clamping plate 722 and a second clamping plate 723 vertically arranged. The first clamping plate 722 and the second clamping plate 723 can be made of a hard material such as plastic, metal, etc. The first clamping plate 722 and the second clamping plate 723 are arranged in a staggered and parallel manner to cooperate with the detection assembly 90. The first clamping plate 722 is provided with a fixing hole 7221 to cooperate with the detection assembly 90. The second clamping plate 723 is provided with a fixing hole 7231 to cooperate with the detection assembly 90. The extension direction of the fixing hole 7221 is parallel to but not coincident with the extension direction of the fixing hole 7231, so as to realize the compact installation of the detection assembly 90. It can be understood that in an embodiment, the mounting seat 721 can be omitted, and the first clamping plate 722 and the second clamping plate 723 can be directly mounted on the bottom body 711.

[0084] The first clamping plate 722 and the second clamping plate 723 are provided with a support plate 724 for supporting the support seat 80. The support plate 724 is arranged opposite to the mounting seat 721.

[0085] In an embodiment, at least part of the structure in the support frame 70, such as the bottom seat 71, can also be part of the rack 10. That is, the support frame 70 can also be part of the rack 10.

[0086] Please refer to Figure 6 , Figure 6 To Figure 5An exploded view of the support seat 80. The support seat 80 can include a support seat body 81 mounted on the base 71 such as the support portion 715 and the support plate 724, a detection cavity assembly 82 mounted on the support seat body 81, a sample cavity assembly 83 mounted on the support seat body 81 and cooperating with the detection cavity assembly 82 to support the detection card, and a second circuit board 84 disposed on the support seat body 81. The detection cavity assembly 82 can be mounted on the support seat 80 opposite the heating assembly 54 to cooperate with the heating assembly 54. The sample cavity assembly 83 can be mounted on the support seat 80 opposite the heating assembly 54 to cooperate with the heating assembly 54. The detection cavity assembly 82 and the sample cavity assembly 83 cooperate to support and heat the detection card. The detection cavity assembly 82 can also be used to detect the excitation light of the detection card. The detection cavity assembly 82 and the sample cavity assembly 83 are electrically connected to the second circuit board 84 to achieve heating control of the detection cavity assembly 82 and the sample cavity assembly 83.

[0087] Please refer to Figure 7 and Figure 8 , Figure 7 and Figure 8 respectively Figure 6 are structural schematic diagrams of the support seat body 81 from different perspectives. The support seat body 81 can be made of a rigid material such as plastic, metal, etc. The support seat body 81 can have a plate-like structure as a whole. The top of the support seat body 81 can be provided with a placement groove 811 to accommodate the card holder 65. The support seat body 81 can be provided with a clamping hole 812 in the placement groove 811 to cooperate with the sample cavity assembly 83. The support seat body 81 can also be provided with a positioning member 813 in the placement groove 811 to cooperate with the card holder 65 to position the detection card. In an embodiment, the positioning member 813 can be a positioning groove provided in the placement groove 811 of the support seat body 81 to position the detection card when the card holder 65 is placed in the positioning member 813 such as the positioning groove. In an embodiment, the shape of the positioning member 813 such as the positioning block can match the shape of the card holder 65 such as the positioning groove. It can be understood that the positioning cooperation relationship between the support seat body 81 and the card holder 65 is not limited to the cooperation relationship between the positioning member 813 and the card holder 65, but can also be a magnetic force between a magnet and another magnet, a magnetic force between an electromagnet and another electromagnet, etc. Of course, it can also be other cooperation relationships, which will not be described in detail.

[0088] The support seat body 81 can be provided with a positioning hole 814 in the placement groove 811 to allow light to pass through the positioning hole 814 and be received by the positioning light receiver 716 to position the detection card.

[0089] The support base body 81 is provided with a plurality of extension grooves 815 around the circumference of the placement groove 811. The extension grooves 815 are communicated with the placement groove 811, so that the support base body 81 forms an assembly platform 816 between two adjacent extension grooves 815. The detection card can be placed on the assembly platform 816, and the extension grooves 815 accommodate the detection card, so that the assembly platform 816 supports and fixes the detection card.

[0090] The support base body 81 is provided with a plurality of extension grooves 815 around the circumference of the placement groove 811. The extension grooves 815 are communicated with the placement groove 811, so that the support base body 81 forms an assembly platform 816 between two adjacent extension grooves 815. The detection card can be placed on the assembly platform 816, and the extension grooves 815 accommodate the detection card, so that the assembly platform 816 supports and fixes the detection card.

[0091] The support base body 81 is provided with a plurality of extension grooves 815 around the circumference of the placement groove 811. The extension grooves 815 are communicated with the placement groove 811, so that the support base body 81 forms an assembly platform 816 between two adjacent extension grooves 815. The detection card can be placed on the assembly platform 816, and the extension grooves 815 accommodate the detection card, so that the assembly platform 816 supports and fixes the detection card.

[0092] Please refer to Figure 6 , the detection cavity assembly 82 is installed in the support base body 81, such as the accommodation hole 817 and the recess 818. The detection cavity assembly 82 can include at least one light detection piece 821, and the specific number of the light detection piece 821 can be one of 1, 2, 3, 4, 5, 6, etc. The detection cavity assembly 82 is installed in the recess 818.

[0093] Please refer to Figure 9 , Figure 9 is a structural schematic view of the light detection piece 821 in Figure 6 . The light detection piece 821 can be provided corresponding to the heating assembly 54, so that the two cooperate to heat one detection card. The light detection piece 821 can include a detection seat 822 placed in the support base body 81, such as the accommodation hole 817 and the recess 818, and a first clamping piece 823 for fixing the detection seat 822 on the support base body 81.

[0094] Please refer to Figure 9 and Figure 10 , Figure 10 is a sectional view of the detection seat 822 along the line X-X in Figure 9 . The detection seat 822 can include a detection seat body 8221 placed in the support base body 81, such as the recess 818. The detection seat body 8221 can be made of hard material, such as plastic, metal, etc. An extension 8222 is provided on the side of the detection seat body 8221 facing the accommodation hole 817, so that the extension 8222 extends into the accommodation hole 817. The extension 8222 is flush with the surface of the assembly platform 816 on the side of the support base body 81 where the assembly platform 816 is provided, so as to improve the appearance performance of the support base 80.

[0095] The extension 8222 is provided with a detection slot 8223, so as to place part of the structure of the detection card in the detection slot 8223 on the side of the support seat body 81 where the assembly table 816 is arranged.

[0096] In an embodiment, the extension 8222 is provided with an isolation slot 8224 on the side of the detection slot 8223 close to the sample cavity assembly 83, so as to place part of the structure of the detection card in the isolation slot 8224 on the side of the support seat body 81 where the assembly table 816 is arranged.

[0097] The detection slot 8223 extends from the extension 8222 to the side of the detection seat body 8221.

[0098] The detection seat body 8221 is provided with an excitation optical fiber channel 8225 in the extension direction of the detection slot 8223, for mounting an excitation optical fiber 8226. The excitation optical fiber channel 8225 is in communication with the detection slot 8223, so that the excitation optical fiber 8226 emits light such as excitation light to the detection slot 8223. In an embodiment, the extension direction of the excitation optical fiber channel 8225 can be consistent with the extension direction of the detection slot 8223. In an embodiment, the extension direction of the excitation optical fiber channel 8225 can form an angle with the extension direction of the detection slot 8223, and the angle can be 0-75°. In an embodiment, the excitation optical fiber channel 8225 is inclined from the detection slot 8223 to the side of the sample cavity assembly 83.

[0099] In an embodiment, the excitation optical fiber channel 8225 is inclined from the detection slot 8223 to the side of the sample cavity assembly 83, and the extension direction of the excitation optical fiber channel 8225 can form an angle with the extension direction of the detection slot 8223, and the angle can be one of 0, 15°, 30°, 45°, 60°, 75°.

[0100] The excitation optical fiber 8226 can include an excitation optical fiber body 8226a and an excitation optical fiber connector 8226b arranged at the end of the excitation optical fiber body 8226a. The excitation optical fiber connector 8226b is placed in the excitation optical fiber channel 8225. The connection between the excitation optical fiber body 8226a and the excitation optical fiber connector 8226b forms a fixing position for cooperating with the first clamping piece 823.

[0101] In an embodiment, the diameter of the excitation optical fiber 8226, such as the excitation optical fiber body 8226a, is about 2.5 mm. Specifically, it can be 2.5 mm.

[0102] The detection seat body 8221 is provided with a receiving optical fiber channel 8227 in communication with the detection slot 8223 at a position forming an angle with the extension direction of the detection slot 8223. The receiving optical fiber 8228 is used to receive the excitation light emitted by the excitation optical fiber 8226 and the fluorescence formed by the excitation light irradiating the detection card.

[0103] In an embodiment, the receiving fiber channel 8227 can be inclined from the detection slot 8223 to a side away from the sample chamber assembly 83, and the angle between the extension direction of the receiving fiber channel 8227 and the extension direction of the detection slot 8223 can be 0-75°. In an embodiment, the excitation fiber channel 8225 is inclined from the detection slot 8223 to a side away from the sample chamber assembly 83.

[0104] In an embodiment, the receiving fiber channel 8227 is inclined from the detection slot 8223 to a side away from the sample chamber assembly 83, and the angle between the extension direction of the receiving fiber channel 8227 and the extension direction of the detection slot 8223 can be one of 0, 15°, 30°, 45°, 60°, and 75°.

[0105] In an embodiment, the excitation fiber channel 8225 is inclined from the detection slot 8223 to a side away from the sample chamber assembly 83, and the angle between the extension direction of the excitation fiber channel 8225 and the extension direction of the detection slot 8223 can be 45°. The receiving fiber channel 8227 is inclined from the detection slot 8223 to a side away from the sample chamber assembly 83, and the angle between the extension direction of the receiving fiber channel 8227 and the extension direction of the detection slot 8223 can be 75°. That is, the angle between the extension direction of the receiving fiber channel 8227 and the extension direction of the excitation fiber channel 8225 can be 120°. The signal-to-noise ratio of the light detection member 821 can be above 80 dB.

[0106] In an embodiment, the extension direction of the excitation fiber channel 8225 can be consistent with the extension direction of the detection slot 8223. The receiving fiber channel 8227 is inclined from the detection slot 8223 to a side away from the sample chamber assembly 83, and the angle between the extension direction of the receiving fiber channel 8227 and the extension direction of the detection slot 8223 can be 60°. That is, the angle between the extension direction of the receiving fiber channel 8227 and the extension direction of the excitation fiber channel 8225 can be 60°. The signal-to-noise ratio of the light detection member 821 can be above 60 dB.

[0107] The detection seat body 8221 is provided with a heating member accommodating slot 8229a on a side where the excitation fiber channel 8225 is arranged, for arranging a heating resistor or other heating device. The detection seat body 8221 can heat the detection card through the heating device. The heating device in the heating member accommodating slot 8229a can be electrically connected with the second circuit board 84, so as to realize heating under the control of the second circuit board 84.

[0108] The detection seat body 8221 is provided with a heating element accommodating groove 8229b on the side close to the sample adding cavity assembly 83, for arranging a heating resistor or other heating device. The detection seat body 8221 can heat the detection card through the heating device. The heating device in the heating element accommodating groove 8229b can be electrically connected with the second circuit board 84, so as to realize heating under the control of the second circuit board 84.

[0109] In an embodiment, the detection seat body 8221 can be an integral structure with the support seat body 81.

[0110] Please refer to Figure 9 The first clamping piece 823 can be made of hard material such as plastic, metal, etc. The first clamping piece 823 can include a clamping body 8231. The clamping body 8231 can be in the form of a strip-shaped plate structure, and of course can also be in other structures, which will not be described herein. The middle part of the clamping body 8231 can abut against the side of the detection seat 822, such as the detection seat body 8221, where the excitation optical fiber channel 8225 is arranged. The two ends of the clamping body 8231 are provided with fixing parts 8232, so as to be fixed with the mounting table 819 on the two sides of the detection seat 822, such as the detection seat body 8221. The fixing can be achieved by plug-in connection, buckling, welding, bonding, etc. A plurality of clamping holes 8233 are arranged on the edge of the middle part of the clamping body 8231, so as to accommodate the excitation optical fiber 8226. The clamping body 8231 forms a clamping tooth 8234 between two adjacent clamping holes 8233. The clamping body 8231 abuts against the side of the detection seat 822, such as the detection seat body 8221, where the excitation optical fiber channel 8225 is arranged, so that the excitation optical fiber joint 8226b is clamped in the excitation optical fiber channel 8225 by the two adjacent clamping teeth 8234, and the excitation optical fiber body 8226a is located between the two adjacent clamping teeth 8234. The fixing of the optical detection element 821 is achieved.

[0111] In an embodiment, in order to accommodate the heating element accommodating groove 8229a, the clamping body 8231 is provided with a through hole 8235 at a position opposite to the heating element accommodating groove 8229a, so that the heating device extends into the heating element accommodating groove 8229a from the through hole 8235.

[0112] Please refer to Figure 11 and Figure 12 , Figure 11 for Figure 6 the structure schematic view of the sample adding cavity assembly 83. Figure 12 for Figure 11A sectional view of the sample adding cavity assembly 83 along the line Xll-Xll. The sample adding cavity assembly 83 can include a supporting plate 831 fixed on the support plate 724, a support assembly 832 mounted on the supporting plate 831, and a sample adding cavity mounting seat 833 mounted on the support assembly 832. The sample adding cavity mounting seat 833 is used for fixing the detection card and heating the detection card. The sample adding cavity mounting seat 833 cooperates with the heating assembly 54 to heat the detection card.

[0113] The supporting plate 831 can be made of a hard material such as plastic, metal, etc. The whole can be in a plate structure, of course, it can also be other structures, which are not described herein. The supporting plate 831 can be fixed on the support plate 724 by screwing, inserting, bonding, welding, etc. In an embodiment, the supporting plate 831 can be fixed on the support seat body 81 by screwing, inserting, bonding, welding, etc. In an embodiment, the supporting plate 831 can be omitted, and the support assembly 832 can be directly fixed on the support plate 724. In an embodiment, when the support plate 724 is omitted, the supporting plate 831 can be directly fixed on the first card fixing plate 722 and the second card fixing plate 723. In an embodiment, when the support plate 724 is omitted, the supporting plate 831 can be fixed on the support part 715.

[0114] The support assembly 832 can include a sleeve column 8321 arranged on the side of the supporting plate 831 away from the support plate 724, and a spring 8322 sleeved on the sleeve column 8321. One end of the spring 8322 abuts against the supporting plate 831, and the other end abuts against the sample adding cavity mounting seat 833, so as to adjust the distance between the sample adding cavity mounting seat 833 and the supporting plate 831. In an embodiment, the sleeve column 8321 can be omitted. In an embodiment, the spring 8322 can be replaced by an elastic member made of other materials. In an embodiment, the sleeve column 8321 can be part of the supporting plate 831. In an embodiment, the sleeve column 8321 can be arranged on the sample adding cavity mounting seat 833. In an embodiment, the spring 8322 can also be replaced by other elastic members such as plastic, torsional spring, metal strip with elastic deformation, etc.

[0115] The sample adding cavity mounting seat 833 is used for placing the detection card. The sample adding cavity mounting seat 833 can include a mounting seat body 8331. The mounting seat body 8331 can be made of a hard and heat-conducting material such as plastic or metal.

[0116] The mounting seat body 8331 is arranged in the support seat body 81, such as the clamping hole 812, to slide in the clamping hole 812. The mounting seat body 8331 is provided with a sample cavity arranging groove 8332 on the side away from the support plate 831, to mount the detection card. The mounting seat body 8331 is provided with a sleeve groove 8333 on the side facing the support assembly 832, to abut against the mounting seat body 8331 when the spring 8322 is arranged in the sleeve groove 8333. In an embodiment, the sleeve groove 8333 can be omitted.

[0117] The mounting seat body 8331 is provided with a clamping edge 8334 on the side facing the support assembly 832, so that the mounting seat body 8331 extends into the clamping hole 812 from the side of the support seat body 81 facing the support assembly 832, to avoid the mounting seat body 8331 from sliding off the support seat body 81 on the side away from the support assembly 832.

[0118] The mounting seat body 8331 is provided with a heating element accommodating groove 8335, to accommodate a heating device such as a heating resistor, so that the mounting seat body 8331 can heat the detection card. The heating device can be electrically connected to the second circuit board 84, to heat under the control of the second circuit board 84. In an embodiment, the heating element accommodating groove 8335 is located between the sleeve groove 8333 and the sample cavity arranging groove 8332.

[0119] In order to better heat the detection card, avoid the influence of condensed water vapor on the detection process after heating, and improve the detection accuracy, the mounting seat body 8331 is provided with an abutting portion 8336 on the side edge of the sample cavity arranging groove 8332, to abut against the detection card. In an embodiment, the abutting portion 8336 can also be used to position the detection card.

[0120] Please refer to Figure 6 The second circuit board 84 can be electrically connected to the heating device in the detection cavity assembly 82 and the heating device in the sample cavity assembly 83, to control the heating of the heating device.

[0121] The second circuit board 84 can be in a ring structure, and can be sleeved around the sample cavity assembly 83. The second circuit board 84 can be directly fixed to the support seat body 81 on the side close to the support frame 70, such as the support plate 724, for example, can be fixed by welding, plugging, buckling, bonding, screwing, etc. In an embodiment, the second circuit board 84 can also be directly fixed to the support frame 70, such as the support plate 724. In an embodiment, the second circuit board 84 can also be directly fixed to the support frame 70, such as the support portion 715. In an embodiment, the second circuit board 84 can also be directly fixed to the sample cavity assembly 83, such as the support plate 831.

[0122] It can be understood that the names of "first circuit board", "second circuit board" and "circuit board" can be converted to each other in some embodiments. For example, in an embodiment, the "first circuit board" in other embodiments is called "second circuit board", and accordingly, the "second circuit board" in other embodiments is called "first circuit board".

[0123] In an embodiment, the second circuit board 84 can be omitted without sharing the working pressure of the control circuit board 40, and the heat generating devices in the detection cavity assembly 82 and the heat generating devices in the sample adding cavity assembly 83 can be directly electrically connected with the control circuit board 40.

[0124] Please refer to Figure 5 . The detection assembly 90 can include a light generator 91 mounted on the support frame 70, such as the base body 711, a light receiving assembly 92 mounted on the support frame 70, such as the base body 711, and a detection cavity assembly 82 mounted on the support seat 80, such as the support seat body 81 (i.e. the detection cavity assembly 82 of the support seat 80 introduced earlier, which can be a common element of the support seat 80 and the detection assembly 90). Among them, the light generator 91 and the light receiving assembly 92 are electrically connected with the control circuit board 40. The light generator 91 is used to generate excitation light, and can generate excitation light under the control of the control circuit board 40. The excitation light can be transmitted to the detection cavity assembly 82 to excite the detection card and generate fluorescence, and the fluorescence can be received by the light receiving assembly 92. The light receiving assembly 92 can generate a detection signal under the control of the control circuit board 40. The detection signal is transmitted to the control circuit board 40 and the control circuit board 40 processes to generate diagnostic data.

[0125] Please refer to Figure 5 and Figure 13 , Figure 13 for Figure 5 the structural schematic diagram of the light generator 91. The number of light generators 91 can be one or more. The number of light generators 91 can be one of 2, 3, 4, 5, 6, etc. In an embodiment, the number of light generators 91 can be 2, specifically a first light generator 911 and a second light generator 912.

[0126] The first light generator 911 and the second light generator 912 can be fixed on the fixed seat 72, such as the mounting seat 721, which can be fixed on the fixed seat 72, such as the mounting seat 721, by plug-in, welding, screwing, bonding, buckling, etc. The excitation light output end of the first light generator 911 can pass through the fixed hole 7221 to be fixed on the first clamping plate 722. The excitation light output end of the second light generator 912 can pass through the fixed hole 7231 to be fixed on the second clamping plate 723.

[0127] Please refer to Figure 14 , Figure 14 forFigure 13 A cross-sectional view of the first light generator 911. The light generator 91, such as the first light generator 911, can include a main housing 913 provided with a receiving cavity 9130, a light source 914 mounted in the receiving cavity 9130, a first lens module 915 mounted in the receiving cavity 9130, a functional film group 916 mounted in the receiving cavity 9130, and an excitation optical fiber 917 extending into the receiving cavity 9130. The light source 914 can be electrically connected to the control circuit board 40 through circuit traces for generating excitation light under the control of the control circuit board 40. The excitation light can be coupled into the excitation optical fiber 917 after sequentially passing through the first lens module 915 and the functional film group 916.

[0128] The main housing 913 can include a front housing 9131 provided with a first sub-receiving cavity 9131a, and a rear housing 9132 coupled to the front housing 9131 and provided with a second sub-receiving cavity 9132a. The first sub-receiving cavity 9131a and the second sub-receiving cavity 9132a constitute the receiving cavity 9130.

[0129] The front housing 9131 can include a front housing body 9133 coupled to the rear housing 9132, and a front housing cover plate 9134 covering the side of the front housing body 9133 away from the rear housing 9132.

[0130] The front housing body 9133 can be made of a rigid material such as plastic, metal, etc. The front housing body 9133 is provided with the first sub-receiving cavity 9131a. The front housing body 9133 is provided with a first accommodating hole 9131b on the side away from the rear housing 9132, which is in communication with the first sub-receiving cavity 9131a, to accommodate the light source 914.

[0131] The front housing cover plate 9134 can be made of a rigid material such as plastic, metal, etc. The front housing cover plate 9134 can be coupled to the front housing body 9133, for example, by welding, buckling, plugging, screwing, etc. The front housing cover plate 9134 can be provided with circuit traces electrically connected to the light source 914 and a first electrical interface 9141 electrically connected to the circuit traces. The first electrical interface 9141 can be provided on the side of the front housing cover plate 9134 coupled to the front housing cover plate 9134. The first electrical interface 9141 can be electrically connected to the control circuit board 40.

[0132] The rear housing 9132 can include a rear housing body 9135 coupled to the front housing 9131, such as the front housing body 9133, and a rear housing cover plate 9136 covering the side of the rear housing body 9135 away from the front housing 9131, such as the front housing body 9133.

[0133] The rear shell body 9135 can be made of a rigid material such as plastic, metal, etc. The rear shell body 9135 is provided with a second sub-receiving cavity 9132a. The rear shell body 9135 can be fixedly connected with the front shell 9131, for example, the front shell body 9133, in a manner such as insertion, buckling, screwing, welding, bonding, etc.

[0134] The rear shell cover plate 9136 can be made of a rigid material such as plastic, metal, etc. The rear shell cover plate 9136 is provided with a second accommodating hole 9136a in communication with the second sub-receiving cavity 9132a for mounting the excitation optical fiber 917.

[0135] The light source 914 can be an LED lamp. Specifically, it can be a blue LED lamp with a peak wavelength of 470 nm. Of course, it can also be other types of light sources. The light source 914 can be electrically connected with the circuit traces on the front shell cover plate 9134 to achieve electrical connection with the control circuit board 40. The light source 914 is arranged on the side of the front shell cover plate 9134 close to the front shell body 9133. The light source 914 is arranged opposite the first accommodating hole 9131b. In an embodiment, the light source 914 can extend into the first accommodating hole 9131b, and of course, it can also extend from the first accommodating hole 9131b into the first sub-receiving cavity 9131a.

[0136] The first lens module 915 is installed in the first sub-receiving cavity 9131a. Its optical axis can be the same as that of the light source 914 so that the excitation light emitted by the light source 914 can pass through to reduce the beam and focal length of the excitation light, thereby better coupling the excitation light into the excitation optical fiber 917. The first lens module 915 can include a first lens 9151, a second lens 9152 having the same optical axis as the first lens 9151, and a first clamping ring 9153 for fixing the first lens 9151 and the second lens 9152. The optical axis of the first lens 9151 is the same as that of the light source 914. The optical axis of the first lens 9151 or the optical axis of the second lens 9152 can be referred to as the optical axis of the first lens module 915.

[0137] In an embodiment, the lenses such as the first lens 9151 and the second lens 9152 are both positive lenses with positive focal lengths. Specifically, the lenses such as the first lens 9151 and the second lens 9152 can be plano-convex lenses. A plano-convex lens is a positive lens that mainly functions in optical systems for beam expansion, imaging, beam collimation, focal collimation, and beam collimation point light source, etc. One of the incident surface and the exit surface of the plano-convex lens is convex, and the other is flat.

[0138] The incident surface of the first lens 9151 is a plane, and the outgoing surface is a convex surface. The incident surface of the second lens 9152 is a convex surface, and the outgoing surface is a plane. The incident surface of the first lens 9151 is arranged opposite to the light source 914. The incident surface of the second lens 9152 is arranged opposite to the outgoing surface of the first lens 9151. The outgoing surface of the second lens 9152 is arranged opposite to the functional film group 916.

[0139] The first clamping ring 9153 can be sleeved around the first lens 9151 and the second lens 9152 to fix the first lens 9151 and the second lens 9152. When the first lens module 915 is arranged in the first sub-receiving cavity 9131a, the side of the first clamping ring 9153 facing the light source 914 abuts against the front shell 9131, for example, the front shell body 9133, and the side of the first clamping ring 9153 away from the light source 914 abuts against the rear shell 9132, for example, the rear shell body 9135, so as to fix the first lens module 915 by the front shell 9131 and the rear shell 9132.

[0140] It can be understood that in some embodiments, the first lens module 915 can also include other types of lenses, such as convex lenses, concave lenses, etc., which will not be described herein. The arrangement of the first lens 9151 and the second lens 9152 inside the first lens module 915 is not limited to the above-mentioned arrangement, and can also be other arrangements, for example, the first lens 9151 and the second lens 9152 can be directly fixed on the front shell 9131, for example, the front shell body 9133, by means of adhesion, insertion, buckling, screwing, etc.

[0141] The functional film group 916 is used for filtering stray light and adjusting excitation light. The functional film group 916 can include a filter 9161, a light homogenizing plate 9162, and a second clamping ring 9163 arranged in the second sub-receiving cavity 9132a.

[0142] The filter 9161 is used to receive excitation light transmitted through the first lens module 915, for example, the second lens 9152, to filter stray light in the excitation light. The filter 9161 is also called a fluorescence filter. The fluorescence filter is a key optical element applied to life science instruments and biomedical science. Its main function is to select and separate the characteristic wavelength spectrum of the excitation light and the emitted fluorescence of the substance in the fluorescence detection and analysis system of biology and medicine, and then observe it through a fluorescence microscope. In an embodiment, the filter 9161 can be an excitation filter. The excitation filter is also called a fluorescence excitation filter (Exciting Filter, Exciter Filter, Excitation Filter), which is a filter that only allows excitation light of a certain wavelength to pass through in a fluorescence microscope or a fluorescence imaging system. Of course, laser light (i.e., the light source 914 can emit laser light) can also be used as excitation light.

[0143] The filter 9161 is in abutment with the rear shell 9132, for example the rear shell body 9135, on the side away from the first lens module 915. The filter 9161 is in abutment with the second clamping ring 9163 on the side close to the first lens module 915, for example the second lens 9152, to achieve the installation and fixation of the filter 9161 and the rear shell 9132. Of course, other ways can also be adopted to fix the filter 9161 on the rear shell 9132, for example the rear shell body 9135, such as adhesion, insertion, buckling, screwing and the like.

[0144] In an embodiment, the filter 9161 can be an excitation filter. Specifically, the filter 9161 can be an excitation filter of EX450-475 (excitation wavelength 450-475 nm).

[0145] The homogenizing plate 9162 is used to receive the excitation light transmitted through the filter 9161. The homogenizing plate 9162, also known as a homogenizing mirror, diffuser or homogenizer, is used to convert the excitation light into a uniform light spot of any shape.

[0146] The homogenizing plate 9162 is used to receive the excitation light transmitted through the filter 9161. The side of the homogenizing plate 9162 close to the filter 9161 is in abutment with the filter 9161, and the side of the homogenizing plate 9162 away from the filter 9161 is in abutment with the rear shell 9132, for example the rear shell cover plate 9136, to achieve the fixation of the homogenizing plate 9162. Of course, other ways can also be adopted to fix the homogenizing plate 9162 on the rear shell 9132, such as adhesion, insertion, buckling, screwing and the like.

[0147] One end of the excitation optical fiber 917 can extend into the rear shell 9132, for example the second accommodating hole 9136a, so that the excitation light transmitted through the homogenizing plate 9162 is coupled into the excitation optical fiber 917. Of course, the excitation optical fiber 917 can also extend into the rear shell 9132, for example the second sub-accommodating cavity 9132a.

[0148] The other end of the excitation optical fiber 917 can be coupled with at least one excitation optical fiber 8226, so that the plurality of light detection pieces 821 can be detected by using the same excitation light, which can reduce the detection error caused by different excitation lights. In an embodiment, the number of light detection pieces 821 is six, and the excitation optical fiber 917 of the first light generator 911 can be coupled with the excitation optical fibers 8226 of three light detection pieces 821. The excitation optical fiber 917 of the second light generator 912 can be coupled with the excitation optical fibers 8226 of three light detection pieces 821.

[0149] In an embodiment, the diameter of the excitation optical fiber 917 is about 2.5 mm. Specifically, it can be 2.5 mm.

[0150] Please refer to Figure 15 , Figure 15 forFigure 5 An exploded view of the light receiving assembly 92. The light receiving assembly 92 can include a light receiving member 921 electrically connected with the control circuit board 40, a light path assembly 922 for optical connection with the detection cavity assembly 82, a first gasket 923 disposed on the light receiving member 921 and used for fixing the light path assembly 922, a second gasket 924 disposed on the first gasket 923 and used for fixing the light path assembly 922, and a clamping assembly 925 disposed on the second gasket 924 and used for fixing the light path assembly 922. Wherein, the fluorescence is transmitted to the light receiving member 921 through the light path assembly 922. The light receiving member 921 generates a detection signal and transmits the detection signal to the control circuit board 40.

[0151] Please refer to Figure 16 , Figure 16 To Figure 5 An assembly view of the light receiving member 921. The light receiving member 921 can include a fixed plate 9211 in a ring shape and arranged with circuit traces, a second electrical interface 9212 disposed on the fixed plate 9211 and electrically connected with the circuit traces, and light sensors 9213 circumferentially distributed on the fixed plate 9211 and electrically connected with the circuit traces. Wherein, the light receiving member 921 can be used for receiving fluorescence. The light receiving member 921 is electrically connected with the control circuit board 40 through the second electrical interface 9212 to transmit a detection signal generated by the fluorescence to the control circuit board 40.

[0152] The fixed plate 9211 can be installed at the isolation groove 713 and the fixed portion 714 on the support frame 70, for example, the base body 711. The fixed plate 9211 can be spaced apart from the base body 711 at the isolation groove 713. Of course, the fixed plate 9211 can also extend into the isolation groove 713 to form a snap structure with the base body 711. The fixed plate 9211 can be connected and fixed with the base body 711 at the fixed portion 714 by screwing, snapping, plugging, bonding, welding, etc. The fixed plate 9211 can surround the fixed groove 712. In an embodiment, the size of the fixed plate 9211 can be similar to, or even identical to, the size of the support seat 80, for example, the support seat body 81, so that the receiving optical fiber channel 8227 of the detection cavity assembly 82 of the support seat 80 is located above the light sensors 9213, or even directly above the light sensors 9213, reducing the difficulty of arranging the receiving optical fiber 8228.

[0153] In an embodiment, the light sensors 9213 are photodiodes.

[0154] Please refer to Figure 15 , the light path assembly 922 can include at least one sub-light path assembly 9221. In an embodiment, the number of sub-light path assemblies 9221 can be consistent with the number of light sensors 9213 in the light receiving member 921, so that the sub-light path assemblies 9221 correspond one-to-one with the light sensors 9213.

[0155] Referring to Figure 17 and Figure 18 , Figure 17 As Figure 15 A structural schematic diagram of the neutron optical path assembly 9221. Figure 18 As Figure 17 A cross-sectional view of the neutron optical path assembly. The neutron optical path assembly 9221 can include a second lens module 9222 that transmits fluorescent light to the light sensor 9213 and a receiving optical fiber 9223 that transmits fluorescent light to the second lens module 9222.

[0156] The second lens module 9222 can include a third lens 9224 mounted in a first gasket 923, a third clamping ring 9225 for fixing the third lens 9224 in the first gasket 923, a fourth clamping ring 9226 mounted in a second gasket 924, and a fourth lens 9227 fixed in the second gasket 924 by the fourth clamping ring 9226.

[0157] It can be understood that the names of "first lens", "second lens", "third lens", "fourth lens" and "lens" can be converted to each other in some embodiments. For example, in an embodiment, the "first lens" in other embodiments is referred to as "second lens", and accordingly, the "second lens" in other embodiments is referred to as "first lens".

[0158] In an embodiment, the lenses, such as the third lens 9224 and the fourth lens 9227, are both positive lenses with positive focal lengths. Specifically, the lenses, such as the third lens 9224 and the fourth lens 9227, can be plano-convex lenses. A plano-convex lens is a positive lens that mainly functions in optical systems for purposes such as beam expansion, imaging, beam collimation, focused collimation, and beam collimation point light source. One of the incident surface and the exit surface of the plano-convex lens is convex, and the other is flat.

[0159] The incident surface of the third lens 9224 is convex, and the exit surface is flat. The incident surface of the fourth lens 9227 is flat, and the exit surface is convex. The incident surface of the third lens 9224 is arranged opposite to the exit surface of the fourth lens 9227. The exit surface of the third lens 9224 is arranged opposite to the light sensor 9213. The incident surface of the fourth lens 9227 is arranged opposite to the receiving optical fiber 9223.

[0160] In one embodiment, an emission filter (also known as an emission filter, barrier filter, or emitter) may be disposed between the third lens 9224 and the fourth lens 9227. An emission filter is a filter used to select and transmit the fluorescence emitted by the substance being detected. It blocks light from emitting any light other than the autofluorescence of the substance being detected. Typically, the wavelength of the emitted light is longer than the wavelength of the excitation light. Narrow-band filters, bandpass filters, or long-pass filters can be selected as emission filters.

[0161] In one embodiment, the emission filter may specifically be an excitation filter with an EM522-645 emission wavelength of 522-645nm.

[0162] The third retaining ring 9225 is disposed on the incident surface side of the third lens 9224 to press the third lens 9224 against the first washer 923, thereby fixing the third lens 9224.

[0163] The fourth retaining ring 9226 is disposed on the exit surface side of the fourth lens 9227 to press the fourth lens 9227 against the second washer 924, thereby fixing the fourth lens 9227.

[0164] Understandably, the terms "first locking ring," "second locking ring," "third locking ring," "fourth locking ring," and "locking ring" can be interchanged in some embodiments. For example, in one embodiment, the "first locking ring" in other embodiments is referred to as the "second locking ring," and correspondingly, the "second locking ring" in other embodiments is referred to as the "first locking ring."

[0165] Understandably, in some embodiments, the second lens module 9222 may also include other types of lenses, such as convex lenses, concave lenses, and other functional films, such as light-diffusing films and filters, which will not be elaborated further. The arrangement of the third lens 9224 and the fourth lens 9227 is not limited to those mentioned above; other methods are also possible. For example, the third lens 9224 can be directly fixed to the first washer 923 by bonding, insertion, snap-fitting, screwing, etc. Similarly, the fourth lens 9227 can be directly fixed to the second washer 924 by bonding, insertion, snap-fitting, screwing, etc.

[0166] In one embodiment, the second lens module 9222 provides an emission filter between the third lens 9224 and the fourth lens 9227. An emission filter is a filter used to select and transmit the fluorescence emitted by the substance being detected. It blocks light from other ranges except for the autofluorescence of the substance being detected. Typically, the wavelength of the emitted light is longer than the wavelength of the excitation light. Bandpass filters, long-pass filters, or similar filters can be selected as emission filters.

[0167] In an embodiment, an emission filter can be disposed between the first gasket 923 and the second gasket 924 to achieve fixed connection of the emission filter.

[0168] The receiving optical fiber 9223 can include a receiving optical fiber body 9223a and a receiving optical fiber joint 9223b disposed at an end of the receiving optical fiber body 9223a. The receiving optical fiber joint 9223b is disposed in the first gasket 923 and / or the second gasket 924 to transmit the fluorescent light to the second lens module 9222, for example, the fourth lens 9227. The receiving optical fiber body 9223a and the receiving optical fiber joint 9223b are connected to form a fixed position to cooperate with the clamping assembly 925. In an embodiment, the receiving optical fiber 9223, for example, the receiving optical fiber body 9223a has a diameter of about 2.5 mm. Specifically, the diameter can be 2.5 mm.

[0169] One end of the receiving optical fiber 9223 can be connected to a receiving optical fiber 8228 to transmit the fluorescent light to the light sensor 9213 through the receiving optical fiber 8228 and the receiving optical fiber 9223.

[0170] Please refer to Figure 19 , Figure 19 To Figure 15 is a structural schematic view of the first gasket 923. The first gasket 923 can be made of a hard material, for example, metal, plastic, etc. The first gasket 923 can have a ring structure, specifically, the shape can be consistent with that of the fixed plate 9211, so that the first gasket 923 and the fixed plate 9211 are stacked.

[0171] The first gasket 923 and the fixed plate 9211 can be fixed together by screwing, welding, buckling, bonding, etc.

[0172] The first gasket 923 is provided with a clearance notch 9231 to accommodate the light receiving member 921, for example, the second electrical interface 9212. When the first gasket 923 and the fixed plate 9211 are stacked, the second electrical interface 9212 can be disposed in the clearance notch 9231.

[0173] The first gasket 923 is uniformly distributed with a plurality of first filling holes 9232 for mounting the second lens module 9222, for example, the third lens 9224 and the third clamping ring 9225. The side of the third lens 9224 facing the light sensor 9213 can abut against the first gasket 923. The side of the third lens 9224 away from the light sensor 9213 can abut against the third clamping ring 9225 to achieve fixed connection of the third clamping ring 9225 and the first gasket 923 to the third lens 9224.

[0174] The first filling hole 9232 can be arranged opposite to the light sensor 9213 so that the light sensor 9213 can receive the fluorescent light. In an embodiment, the light sensor 9213 can extend into the first filling hole 9232. The exit surface of the third lens 9224 can abut against the first gasket 923. The third clamping gasket 9225 can be fixedly connected with the first gasket 923 to achieve the connection and fixation of the third lens 9224.

[0175] Referring to Figure 20 , Figure 20 for Figure 15 the structure of the second gasket 924. The second gasket 924 can be made of hard material such as metal, plastic, etc. The second gasket 924 can have a ring structure, which can be consistent with the shape of the first gasket 923 so that the first gasket 923 and the second gasket 924 are arranged in layers. The second gasket 924 is provided with a relief notch 9241 arranged opposite to the relief notch 9231 to provide relief for the second electrical interface 9212. When the second gasket 924, the first gasket 923 and the fixed plate 9211 are arranged in layers, the second electrical interface 9212 can be placed in the relief notch 9231 and the relief notch 9241.

[0176] The first gasket 923 and the second gasket 924 can be fixed together by screwing, welding, buckling, bonding, etc. In an embodiment, the first gasket 923 and the second gasket 924 can be an integral structure.

[0177] The second gasket 924 is provided with a plurality of second filling holes 9242 distributed around the circumference for mounting the second lens module 9222 such as the fourth lens 9227 and the fourth clamping gasket 9226. The second filling hole 9242 can be arranged opposite to the first filling hole 9232 so that the light sensor 9213 can receive the fluorescent light. The side of the fourth lens 9227 facing the light sensor 9213 can abut against the fourth clamping gasket 9226. The side of the fourth lens 9227 away from the light sensor 9213 can abut against the second gasket 924 to achieve the fixed connection of the fourth clamping gasket 9226 and the second gasket 924 to the fourth lens 9227. The receiving optical fiber joint 9223b of the receiving optical fiber 9223 can extend from the second gasket 924 away into the second filling hole 9242.

[0178] It can be understood that the first gasket 923 and the second gasket 924 can form an assembly housing, and the first filling hole 9232 and the second filling hole 9242 communicate to form a filling hole.

[0179] Referring to Figure 15 , the clamping assembly 925 is arranged on the side of the second gasket 924 away from the first gasket 923 to fix the receiving optical fiber 9223. The clamping assembly 925 can include a plurality of second clamping pieces 9251 distributed around the circumference. Referring to Figure 21 , Figure 21For Figure 15 A structural schematic view of the second clamping piece 9251. The second clamping piece 9251 can be made of hard material such as plastic, metal, etc. The second clamping piece 9251 can include a clamping body 9252. The clamping body 9252 can be in a strip-shaped structure so as to be arranged on the second gasket 924. The clamping body 9252 is arranged with a plurality of clamping holes 9253 in the extending direction thereof. The clamping holes 9253 are arranged extending inwardly from the edge. Adjacent two clamping holes 9253 are a clamping tooth 9254. By attaching the clamping body 9252 to the second gasket 924 away from the first gasket 923, the receiving optical fiber connector 9223b is clamped in the second filling hole 9242 by the clamping tooth 9254, and the receiving optical fiber body 9223a is located between adjacent two clamping teeth 9254. The fixed connection between the receiving optical fiber 9223 and the second gasket 924 is achieved.

[0180] It can be understood that the names of "first clamping piece", "second clamping piece" and "clamping piece" can be converted to each other in some embodiments. For example, in an embodiment, the "first clamping piece" in other embodiments is called "second clamping piece", and correspondingly, the "second clamping piece" in other embodiments is called "first clamping piece".

[0181] The clamping body 9252 is arranged with a fixing portion 9255 so as to achieve the fixed connection between the clamping body 9252 and the second gasket 924. In an embodiment, the fixing portion 9255 can be a through hole so as to be screwed and fixed by a screw bolt or the like structure passing through the fixing portion 9255, then passing through the second gasket 924, the first gasket 923, and then being screwed and fixed with the optical receiving member 921 such as the fixing plate 9211.

[0182] It can be understood that the clamping body 9252 can also be connected and fixed with the second gasket 924 in other ways such as plug-in, buckle, etc., which will not be described herein.

[0183] In an embodiment, all the second clamping pieces 9251 in the clamping assembly 925 are an integral structure.

[0184] Please refer again to Figure 1 and Figure 2 The control circuit board 40 can include a first sub-control circuit board 41 fixed on the rack 10 such as the rack main body 11 and a second sub-control circuit board 42 arranged on the top of the rack 10 such as the rack main body 11. Among them, the first sub-control circuit board 41 is electrically connected with the second sub-control circuit board 42.

[0185] The first sub-control circuit board 41 can be electrically connected with the rack 10 such as the driving device 12 so as to control the sliding position of the detection card conveying seat 20 relative to the rack 10.

[0186] The first sub-control circuit board 41 can be electrically connected with the pressing disc 50, such as the first circuit board 55, to control the electromagnetic member 52 and the magnetic force adsorption of the pressing member 53, so as to control the heating assembly 54 to heat the detection card.

[0187] The first sub-control circuit board 41 can be electrically connected with the card conveying assembly 60, so as to control the third driving member 64 and the card holder 65 to slide relative to the sliding frame 61.

[0188] The first sub-control circuit board 41 can be electrically connected with the card conveying assembly 60, such as the third driving member 64, to control the third driving member 64 to drive the card holder 65 to centrifugally process the detection card.

[0189] The first sub-control circuit board 41 can be electrically connected with the detection cavity assembly 82, such as the heating device, to control the detection seat 822 to heat the detection card.

[0190] The first sub-control circuit board 41 can be electrically connected with the sample adding cavity assembly 83, such as the heating device, to control the sample adding cavity mounting seat 833 to heat the detection card.

[0191] The first sub-control circuit board 41 can be electrically connected with the detection assembly 90, such as the light source 914, to control the light source 914 to emit excitation light.

[0192] The first sub-control circuit board 41 can be electrically connected with the detection assembly 90, such as the light sensor 9213, to control the light sensor 9213 to receive fluorescence.

[0193] The first sub-control circuit board 41 can be electrically connected with the rack 10, such as the limit switch, the detection card conveying seat 20, such as the light sensor, to control the position of the third driving member 64 driving the card holder 65 to slide in the card conveying member 62.

[0194] The first sub-control circuit board 41 can be electrically connected with the rack 10, such as the limit switch, to control the sliding position of the card conveying assembly 60.

[0195] The first sub-control circuit board 41 can be electrically connected with the card conveying member 62, such as the positioning light generator, the support frame 70, such as the positioning light receiver 716, to control the position of the detection card.

[0196] The second sub-control circuit board 42 can be installed above the rack 10, such as the rack main body 11. In an embodiment, when the molecular diagnostic device 100 is provided with a cabinet, the second sub-control circuit board 42 can be provided at a position opposite to the rack main body 11 of the cabinet.

[0197] The second sub-control circuit board 42 can be electrically connected with an output device, such as a display or a printer, to output the diagnostic data of the molecular diagnostic device 100 through the output device.

[0198] The second sub-control circuit board 42 can be electrically connected with a display, a keyboard, a code scanning device and other input devices, so as to input control instructions to the molecular diagnostic device 100, for example, the control circuit board 40, through the input devices, so that the molecular diagnostic device 100 controls the detection card conveying seat 20 and / or the detection card detection seat 30 through the control circuit board 40.

[0199] In an embodiment, the second sub-control circuit board 42 and the first sub-control circuit board 41 can omit one of them, and integrate the two on one sub-control circuit board.

[0200] Next, a detection card is described, which can be used in the molecular diagnostic device 100 in the above embodiments, to complete the detection of the sample loaded on the detection card, and further process to form diagnostic data.

[0201] Please refer to Figure 22 , Figure 22 which is a structural schematic diagram of the detection card in an embodiment of the present application. The detection card 93 is also called a molecular diagnostic centrifugal detection card or a test card. The detection card 93 can include a body 94 provided with a sample adding cavity, a flow channel, a waste liquid cavity, an isolation cavity and a detection cavity, an isolation layer 95 covering one side of the body 94, and a cover 96 covering the sample adding cavity of the body 94.

[0202] Please refer to Figure 22 , Figure 23 and Figure 24 , Figure 23 which is a sectional view of the detection card 93 along line L-L in Figure 22 , Figure 24 which is a three-dimensional structural schematic diagram of the detection card 93 in Figure 22 . The body 94 is made of a hard material such as plastic. Specifically, the body 94 can be made of ABS (Acrylonitrile Butadiene Styrene plastic), PDMS (Polydimethylsiloxane), PC (Polycarbonate), PMMA (Polymethyl methacrylate), PS (General purpose polystyrene), PP (Polypropylene), COC (copolymers of cycloolefin) or COP (Cyclo Olefin Polymer), etc. material, which is processed by injection molding, numerical control machine tool processing or 3D printing and other processing methods.

[0203] The body 94 can be a plate structure as a whole. It can be generally a fan shape, specifically a fan ring shape, a fan blade shape or a pie shape. For example, the body 94 can be a fan shape formed by two straight edges and an arc edge connected in sequence. For example, the body 94 can be a fan ring shape formed by a straight edge and an outer arc edge, a straight edge and an inner arc edge connected in sequence. Of course, the body 94 can also be other shapes, which will not be described here.

[0204] In an embodiment, the two straight edges of the fan shape of the body 94 can form an angle of 40°-60°, the inner arc edge can have a diameter of 10mm-100mm, and the outer arc edge can have a diameter of 100mm-200mm. With such a size and structure, the detection card 93 can be arranged in at least 6 in the detection plane of the molecular diagnostic device 100 to form a circular plane, so that at least 6 detection cards 93 can be detected at the same time, improving the overall detection efficiency and being able to meet the detection requirements of a larger scale.

[0205] The body 94 is provided with a mounting portion 941 near the center of the outer arc edge or the inner arc edge and protruding away from the isolation layer 95. The body 94 is recessed at a position opposite to the mounting portion 941 near the isolation layer 95 to form a sample adding cavity 9411 for sample addition at the sample adding cavity 9411. The sample adding cavity 9411 is mainly used for pretreatment of the sample (liquid sample), and the pretreatment method can include one or more, such as chemical treatment, heat treatment, enzyme treatment and physical separation, etc. In some embodiments, the volume of the sample adding cavity 9411 is generally 200-2000μl. Dry reagents can be preloaded in the sample adding cavity 9411, which can be dried in situ or added to the sample adding cavity 9411 as lyophilized reagents.

[0206] The body 94 is provided with a clamping portion 942 at a position away from the isolation layer 95 and near the outer arc edge to cooperate with the shipping assembly 60, such as the card holder 65. In an embodiment, the clamping portion 942 can be a protrusion.

[0207] The first limiting portion 943 of the main body 94 is arranged at the edge of the straight line edge close to the outer arc edge and is bent to the side away from the isolation layer 95. The second limiting portion 944 of the main body 94 is arranged at the edge of the straight line edge close to the outer arc edge and is protruded to the side away from the isolation layer 95. The first limiting portion 943 and the second limiting portion 944 of the main body 94 cooperate to fix the detection card 93, so as to smoothly perform the centrifugal treatment of the detection card 93. The waste liquid cavity 9441 is recessed at the side close to the isolation layer 95 and is opposite to the second limiting portion 944. A plurality of plug-in portion groups are uniformly distributed along the extension direction of the outer arc edge at the side away from the isolation layer 95 and between the first limiting portion 943 and the second limiting portion 944 of the main body 94. Each plug-in portion group can include a first plug-in portion 945 and a second plug-in portion 946. The line between the first plug-in portion 945 and the second plug-in portion 946 can pass the center of the outer arc edge. The first plug-in portion 945 is arranged between the mounting portion 941 and the second plug-in portion 946. The first plug-in portion 945 and the second plug-in portion 946 can cooperate with the detection card detection seat 30, such as the isolation groove 8224 and the detection groove 8223.

[0208] The isolation cavity 9451 is recessed at the side close to the isolation layer 95 and is opposite to the first plug-in portion 945 of the main body 94. The isolation cavity 9451 is provided with a meltable isolation body. The isolation body can be switched between a melted state and an un-melted state (usually a solid state). When the detection card 93 is not used for detection, the isolation body can be controlled to be in the un-melted state, and at this time, the isolation body can prevent the sample from entering the detection cavity 9461 (as shown in Figure 24 In some embodiments, the isolation body can be paraffin, microcrystalline wax, synthetic wax or natural wax.

[0209] The detection cavity 9461 is recessed at the side close to the isolation layer 95 and is opposite to the second plug-in portion 946 of the main body 94. The detection cavity 9461 is provided with a reagent. The isolation cavity 9451 and the detection cavity 9461 are in communication at the side close to the isolation layer 95. The isolation body in the isolation cavity 9451 can also be used to seal the reagent to prevent the reagent from entering the isolation cavity 9451 in the opposite direction. The reagent is maintained in the detection cavity 9461. When the test is performed, the isolation body can be controlled to be in the melted state, and at this time, the sample can enter the detection cavity 9461 through the sample adding cavity 9411 to react with the reagent in the detection cavity 9461 to complete the detection.

[0210] The reagent and the meltable isolation body can be stacked in the detection cavity 9461. In some embodiments, the isolation body can be paraffin, microcrystalline wax, synthetic wax or natural wax. The isolation body is characterized by being solid at room temperature and low temperature, and being liquid after being heated to a specific temperature, and having no inhibitory effect on the nucleic acid amplification reaction. In some embodiments, the reagent can be a dry reagent, which includes one or more of primers and DNA binding dyes, enzymes, magnesium sulfate, potassium chloride, dNTPs for amplification reaction. The dry reagent is loaded into the detection cavity 9461 in a liquid state, and the dry reagent is formed by a drying process, the temperature of the drying process is less than the melting temperature of the isolation body, and the drying process includes air drying, oven drying, freeze drying. During the detection heating process, the reagent and the isolation body are both in a liquid state, and due to the smaller specific gravity of the isolation body than the reagent, the isolation body is displaced out of the detection cavity 9461 under the action of the centrifugal field, so as not to affect the reaction and detection.

[0211] In some embodiments, the isolation body is loaded into the detection cavity 9461 in a molten state and is formed by natural solidification or temperature reduction solidification. When not tested, the isolation body can be controlled to be in an unmelted state, and at this time the reagent can be sealed and stored by the isolation body. When testing is needed, the isolation body can be controlled to be in a molten state, for example, by heating the detection card 93, so that the isolation body is heated and melted, at this time, the isolation body can be removed from the detection cavity 9461 and flow to the isolation cavity 9451 under the action of the centrifugal force, and the sample can enter the detection cavity 9461, and then the isolation body is solidified again to block the mouth of the detection cavity 9461, thereby forming mutual isolation and sealing of the plurality of detection cavities 9461, so as to independently perform reaction or testing between the detection cavities 9461.

[0212] The body 94 is provided with a flow channel 947 on the side close to the isolation layer 95, so as to communicate the sample loading cavity 9411 and the isolation cavity 9451.

[0213] The body 94 is provided with an abutting groove 948 on the side away from the isolation layer 95 and opposite to the flow channel 947, so as to cooperate with the abutting portion 8336. For example, the abutting portion 8336 can be placed in the abutting groove 948 to heat the part of the flow channel 947, so as to avoid condensation of water vapor in the flow channel 947, thereby reducing the influence on the subsequent detection process and improving the detection accuracy. In some embodiments, the abutting groove 948 can be omitted.

[0214] The isolation layer 95 can be a film structure, and can also be other structures. The isolation layer 95 can be made of pressure-sensitive adhesive, ultraviolet curing adhesive, or optical-grade double-sided adhesive, and can also be similar to the material of the body 94. The isolation layer 95 can be attached to the body 94, and can be fixed by ultrasonic welding, laser welding, adhesive sealing, or the like, to isolate the flow channel 947, the waste liquid chamber 9441, the isolation chamber 9451, and the detection chamber 9461.

[0215] The cover 96 covers the mouth of the sample adding chamber 9411, and seals the sample adding chamber 9411. When it is necessary to add a sample, the cover 96 can be opened, the sample can be added to the sample adding chamber 9411, and then the cover 96 can be closed. The cover 96 can be water-resistant and air-permeable, which can discharge water vapor generated during heating, reduce the air pressure in the test card 93, and ensure good air permeability. In addition, the cover 96 can prevent aerosols and biological molecules generated during the amplification reaction from escaping, and avoid pollution of the personnel and the environment.

[0216] When the test card 93 is centrifuged, the sample liquid passes through the sample adding chamber 9411 and the flow channel 947. At this time, the isolation body is in a solid state, and the detection chamber 9461 is sealed, so that the sample liquid cannot flow into the detection chamber 9461. After the sample liquid is heated, the isolation body melts and flows into the detection chamber 9461, so that the flow channel 947 and the detection chamber 9461 are connected, and the sample liquid can flow into the detection chamber 9461. Because the specific gravity of the isolation body is smaller than that of the reagent, under the action of the centrifugal field, the isolation body is displaced above the reagent, and does not affect the reaction and detection. In addition, the detection chamber 9461 can be sealed.

[0217] The above describes the preferred embodiments of the present application. It should be noted that, for those skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, which are also considered to be within the scope of protection of the present application.

Claims

1. A test card holder for molecular diagnostic equipment, characterized in that, include: A light generator for generating excitation light, the light generator comprising: A light source used to emit excitation light; A first lens module is used to receive and transmit the excitation light; and A functional diaphragm assembly is used to receive and transmit the excitation light transmitted through the first lens module; An excitation optical fiber is used to transmit the excitation light that passes through the functional diaphragm group and to irradiate the detection card with the excitation light; A receiving optical fiber is used to receive fluorescence generated by the excitation light illuminating the detection card; and An optical receiving component is used to receive the fluorescence transmitted from the receiving optical fiber, the optical receiving component comprising: The second lens module is used to receive the fluorescence transmitted from the receiving optical fiber; and A light sensor is used to receive the fluorescence transmitted through the second lens module; A support frame, on which the light generator and the light receiving assembly are mounted; The support body is disposed on the support frame and is used to support the detection card on the side away from the light generator and the light receiving component. The detection card is provided with a sample application chamber and a detection chamber. Multiple detection seats are arranged around the support body, and the detection seats are used to install the detection cavity; the excitation optical fiber connects the light generator and the detection seats, and the receiving optical fiber connects the light receiving component and the detection seats; A sample dispensing chamber assembly is disposed on the support body and is used to install the sample dispensing chamber and to heat the detection card.

2. The detection card holder for molecular diagnostic equipment according to claim 1, characterized in that, The light generator also includes: The main housing has a receiving cavity, in which the light source, the first lens module, and the functional diaphragm group are disposed, and one end of the excitation optical fiber is placed in the receiving cavity.

3. The detection card holder for molecular diagnostic equipment according to claim 2, characterized in that, The main housing includes: The front housing has a first sub-receiving cavity, and the light source and the first lens module are disposed within the first sub-receiving cavity; and The rear housing is connected and fixed to the front housing and is provided with a second sub-receiving cavity. The functional diaphragm group is disposed in the second sub-receiving cavity, and one end of the excitation optical fiber is placed in the second sub-receiving cavity. The first sub-receiving cavity and the second sub-receiving cavity are connected to form the receiving cavity.

4. The detection card holder for molecular diagnostic equipment according to claim 3, characterized in that, The front housing includes: A front housing body, the first sub-receiving cavity being disposed on the front housing body, the front housing body being fastened to the rear housing to secure the first lens module to the rear housing, the front housing body having a first receiving hole on the side away from the rear housing, the first receiving hole communicating with the first sub-receiving cavity; and A front housing cover is provided on the side of the front housing body away from the rear housing, and the light source is located on the front housing cover opposite to the first receiving hole.

5. The detection card holder for molecular diagnostic equipment according to claim 4, characterized in that, The rear housing includes: A rear housing body, the second sub-receiving cavity disposed on the rear housing body, the rear housing body being fastened to the front housing body to secure the first lens module therewith, and the functional diaphragm assembly disposed within the second sub-receiving cavity; and The rear housing cover plate is disposed on the side of the rear housing body away from the front housing body, and is provided with a second receiving hole communicating with the second sub-receiving cavity, and the excitation optical fiber is disposed in the second receiving hole.

6. The detection card holder for molecular diagnostic equipment according to claim 1, characterized in that, The optical sensors are multiple, and the optical receiving component further includes: A fixing plate surrounds the light generator, and a plurality of light sensors are disposed on the fixing plate and arranged along the direction in which the fixing plate surrounds the light generator; A component housing, surrounding the light generator, is disposed on the mounting plate. The component housing has a filling hole at a position corresponding to the light sensor. A second lens module is disposed within the filling hole, and the receiving optical fiber is disposed within the filling hole. A first clamping piece is disposed on the side of the component housing away from the fixing plate. The filling hole extends from the fixing plate toward the clamping piece. The first clamping piece is used to secure the receiving optical fiber in the filling hole.

7. The detection card holder for molecular diagnostic equipment according to claim 6, characterized in that, The component housing includes: A first washer, surrounding the light generator, is disposed on the mounting plate, and the first washer has a first filling hole at a position corresponding to the light sensor; and A second washer is disposed around the light generator on the first washer. The second washer has a second filling hole at a position corresponding to the first filling hole. The first filling hole and the second filling hole communicate to form the filling hole.

8. The detection card holder for molecular diagnostic equipment according to claim 6, characterized in that, The first clamping piece includes a clamping body, and the clamping body of the first clamping piece is provided with a clamping hole. The receiving optical fiber is placed in the clamping hole of the first clamping piece to secure the receiving optical fiber in the filling hole.

9. The detection card holder for molecular diagnostic equipment according to claim 1, characterized in that, The support body has an assembly platform on the side away from the light generator and the light receiving component. The assembly platform is used to install the detection cavity, and the detection seat is installed on the assembly platform.

10. The detection card holder for molecular diagnostic equipment according to claim 9, characterized in that, The assembly table is provided with a receiving hole, and the testing seat is installed in the receiving hole.

11. The detection card holder for molecular diagnostic equipment according to claim 10, characterized in that, The main body of the support base has a recessed portion on the side near the light generator and the light receiving component. The recessed portion communicates with the receiving hole, and the detection seat is located in the recessed portion.

12. The detection card holder for molecular diagnostic equipment according to claim 11, characterized in that, The detection seat includes: The detection seat body is placed in the recessed portion and has an extension portion placed in the receiving hole. The extension portion has a detection groove to accommodate the detection cavity.

13. The detection card holder for molecular diagnostic equipment according to claim 12, characterized in that, The detection base body is provided with an excitation fiber channel, which is connected to the detection slot, and the excitation fiber is installed in the excitation fiber channel.

14. The detection card holder for molecular diagnostic equipment according to claim 13, characterized in that, The extension direction of the excitation fiber channel forms an angle with the extension direction of the detection groove, and the angle is 0-75°.

15. The detection card holder for molecular diagnostic equipment according to claim 12, characterized in that, The main body of the detection seat is provided with a receiving optical fiber channel, which is connected to the detection slot, and the receiving optical fiber is installed in the receiving optical fiber channel.

16. The detection card holder for molecular diagnostic equipment according to claim 15, characterized in that, The extension direction of the receiving optical fiber channel forms an angle with the extension direction of the detection slot, and the angle is 0-75°.

17. The detection card holder for molecular diagnostic equipment according to claim 16, characterized in that, The support base body is provided with mounting platforms on both sides of the recessed portion, and the support base further includes: The second clamping piece is disposed on the side of the detection seat near the light generator and the light receiving assembly, and is mounted on the mounting platform to fix the detection seat.

18. The test card holder for molecular diagnostic equipment according to claim 17, characterized in that, The second clamping piece includes a clamping body, and the clamping body of the second clamping piece is provided with a clamping hole. The excitation optical fiber is placed in the clamping hole of the second clamping piece to secure the excitation optical fiber in the excitation optical fiber channel.

19. The test card holder for a molecular diagnostic device according to any one of claims 1-18, characterized in that, The light source is a blue LED lamp with a peak wavelength of 470nm.

20. The test card holder for a molecular diagnostic device according to any one of claims 1-18, characterized in that, The first lens module includes: The first lens is a plano-convex lens with a flat incident surface and a convex exit surface. The incident surface of the first lens is used to receive the excitation light emitted by the light source; and The second lens is a plano-convex lens with a convex incident surface and a flat exit surface. The incident surface of the second lens is used to receive the excitation light emitted from the exit surface of the first lens.

21. The test card holder for a molecular diagnostic device according to any one of claims 1-18, characterized in that, The functional membrane assembly includes: An excitation filter is used to receive the excitation light transmitted through the first lens module, wherein the excitation filter is an excitation filter with an excitation wavelength of 450-475 nm; and A light homogenizer is used to receive the excitation light that has passed through the excitation filter.

22. The test card holder for a molecular diagnostic device according to any one of claims 1-18, characterized in that, The second lens module includes: The third lens is a plano-convex lens with a flat incident surface and a convex exit surface. The incident surface of the third lens is used to receive the fluorescence transmitted by the receiving optical fiber; and The fourth lens is a plano-convex lens with a convex incident surface and a flat exit surface. The incident surface of the fourth lens is used to receive the fluorescence emitted from the exit surface of the third lens.

23. The detection card holder for molecular diagnostic equipment according to claim 22, characterized in that, The second lens module also includes: An emission filter is placed between the third lens and the fourth lens to transmit the fluorescence. The emission filter is an excitation filter with an emission wavelength of 522-645nm.

24. The test card holder for a molecular diagnostic device according to any one of claims 1-18, characterized in that, The extension direction of the end of the excitation optical fiber that emits the excitation light forms an angle of 120° with the extension direction of the end of the receiving optical fiber that receives the fluorescence.

25. The test card holder for a molecular diagnostic device according to any one of claims 1-18, characterized in that, The extension direction of the end of the excitation optical fiber that emits the excitation light forms an angle of 60° with the extension direction of the end of the receiving optical fiber that receives the fluorescence.

26. A molecular diagnostic device, characterized in that, include: A support base for supporting a test card, the test card including a sample dispensing chamber and a test chamber; as well as A detection component is used to detect the detection cavity, the detection component comprising: A light source used to emit excitation light; A first lens module is used to receive and transmit the excitation light; A functional diaphragm assembly is used to receive and transmit the excitation light transmitted through the first lens module; An excitation fiber is used to transmit the excitation light that passes through the functional diaphragm assembly. One end of the excitation fiber is disposed on the support to irradiate the detection cavity with the excitation light. A receiving optical fiber, one end of which is mounted on the support base, is used to receive the fluorescence generated by the excitation light irradiating the detection cavity; A second lens module is used to receive the fluorescence transmitted from the receiving optical fiber; and A light sensor is used to receive the fluorescence transmitted through the second lens module; A support frame, on which the detection component is mounted, and a support base is mounted on which the detection card is supported on the side away from the detection component; The support base includes: Multiple detection seats are arranged around the detection chamber; the detection seats are used to install the detection chamber; the excitation optical fiber is connected to the detection seat and extends to the functional diaphragm group to cooperate with the functional diaphragm group; the receiving optical fiber is connected to the detection seat and extends to the second lens module to cooperate with the second lens module. A sample dispensing chamber assembly is used to install the sample dispensing chamber and to heat the detection card.

27. A molecular diagnostic device, characterized in that, include: A light source used to emit excitation light; A first lens module is used to receive and transmit the excitation light; A functional diaphragm assembly is used to receive and transmit the excitation light transmitted through the first lens module; An excitation optical fiber is used to transmit the excitation light that passes through the functional diaphragm group and to irradiate the detection card with the excitation light; A receiving optical fiber is used to receive fluorescence generated by the excitation light illuminating the detection card; as well as An optical receiving component is configured to receive the fluorescence transmitted via the receiving optical fiber, the optical receiving component comprising: The component housing has a filling hole that penetrates the component housing, and one end of the receiving optical fiber is placed in the filling hole; An optical path assembly is disposed within the filling hole for receiving the fluorescence transmitted from the receiving optical fiber; A light receiver, fixed to one side of the component housing, is equipped with a light sensor, which is positioned opposite the filling hole to receive the fluorescence transmitted through the light path component; and A clamping piece is disposed on the side of the component housing away from the optical receiver. The clamping piece is fixedly connected to the component housing to secure the receiving optical fiber in the filling hole. The support frame, on which the light source, the first lens module, the functional diaphragm group and the light receiving component are mounted; The support body is disposed on the support frame and is used to support the detection card on the side away from the light receiving component. The detection card is provided with a sample dispensing chamber and a detection chamber. Multiple detection seats are arranged around the support body, and the detection seats are used to install the detection cavity; the excitation optical fiber is connected to the detection seat and extends toward the functional diaphragm group to cooperate with the functional diaphragm group; the receiving optical fiber is connected to the light receiving component and the detection seat. A sample dispensing chamber assembly is disposed on the support body and is used to install the sample dispensing chamber and to heat the detection card.

Images