A pump valve mechanism, centrifugal device and centrifuge for gas control of microfluidic chip

Abstract

The present invention discloses a pump-valve mechanism for gas control of a microfluidic chip, a centrifugal device, and a centrifuge. The pump-valve mechanism comprises: a turntable housing, a microfluidic chip being fixed at its top, and an air nozzle A and an air nozzle B being provided on the microfluidic chip for supplying and exhausting air to the inner and outer air control channels, respectively; an interface adapter, the pump-valve mechanism being fixed inside the turntable housing, the interface adapter having an A connector and a B connector, the upper end of the A connector being plugged into and connected to the A nozzle, and the upper end of the B connector being plugged into and connected to the B nozzle; and a pump-valve assembly, the pump-valve mechanism being fixed inside the turntable housing, the two air outlets of the pump-valve assembly being connected to the lower ends of the A connector and the B connector, respectively. The pump-valve mechanism is arranged below the microfluidic chip and inside the turntable housing, so that the air pipe and pump body for gas supply are not exposed, thereby improving the overall aesthetics of the structure. In addition, there are no other components above the microfluidic chip, making it convenient to add other devices for chemical reaction detection.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, and more particularly to a pump valve mechanism, a centrifugal device and a centrifuge for gas control of a microfluidic chip. Background Art

[0002] Microfluidics chip technology integrates the basic operating units of biological, chemical, and medical analysis processes, such as sample preparation, reaction, separation, and detection, onto a micron-scale chip. Using centrifugal force as the driving force for liquid flow, the chip uses centrifugal force to achieve the entire process of liquid flow detection and analysis. Due to its unique technical characteristics and advantages, microfluidic chip technology is increasingly attracting the attention of researchers around the world, and has demonstrated potential application prospects in various fields such as life sciences, medical diagnosis, food safety, and animal and plant quarantine. In the field of biomedical diagnosis, the significant advantages of microfluidic chips are: through automated, streamlined working modes, they significantly shorten sample processing time, improve detection efficiency, and reduce the consumption of reaction reagents and samples, ultimately achieving automated, low-cost, and intelligent rapid medical testing.

[0003] In the prior art, for example, the patent CN214937510U, which has been authorized by the applicant, discloses a device and a kit for high-throughput hybridization capture, which discloses a microfluidic chip. The microfluidic chip includes a matching reaction pool mechanism, a membrane mechanism, and a gas control mechanism. The reaction pool mechanism includes: a sample addition pool, a plurality of radially arranged reaction pool groups, and a waste liquid pool. Each column of the reaction pool group includes reaction pool one, reaction pool two, and reaction pool three. The sample addition pool, reaction pool one, and reaction pool two are connected in series by a connecting groove. There is a spacing area (not directly connected) between reaction pool two and reaction pool three, and between reaction pool three and the waste liquid pool. The membrane mechanism is a membrane with ductility (elasticity) and is located between the reaction pool mechanism and the gas control mechanism. The gas control mechanism includes an inner circle gas control channel and an outer circle gas control channel. When the mechanism contacts the membrane mechanism, a gas control valve area is formed. The gas control valve area expands after gas is introduced through the gas control channel and contracts after gas is extracted. In each radially arranged reaction cell group, each space between reaction cells two and three corresponds to a corresponding gas-controlled valve area on the inner ring of gas-controlled channels. The space between reaction cell three and the waste liquid tank corresponds to a corresponding gas-controlled valve area on the outer ring of gas-controlled channels. These valve areas control the flow of liquid in these spaces by controlling the deformation of the membrane mechanism. In the gas-controlled mechanism, the inner and outer rings of gas-controlled channels are independently connected to the gas supply and extraction devices.

[0004] The above-mentioned gas supply and extraction devices, such as Figure 1As shown, it is generally composed of two air pipes 100, a slip ring 200, and two air pumps 300. The slip ring 200 is arranged at the top of the centrifuge 500 and above the microfluidic chip. The two air holes on the microfluidic chip 400 are respectively connected to the two air outlets on the slip ring 200, and the two air inlets on the slip ring 200 are respectively connected to the external air pump 300 through air pipes. In this way, the microfluidic chip can be inflated by the air pump. However, this arrangement structure has limitations. If the number of air pipes is too large, the air pipes will be entangled. Moreover, the air pipes and air pumps are exposed to the outside of the centrifuge, which is not only unsightly and messy, but also the air pipes on the microfluidic chip occupy the space above the microfluidic chip, which is not conducive to adding other chemical reaction detection devices on the microfluidic chip, such as a temperature control device for providing a cooling or heating analysis environment for the microfluidic chip above the microfluidic chip, which is inconvenient to use.

[0005] Therefore, how to provide a microfluidic chip gas control pump valve mechanism and centrifugal device, centrifuge, in which the trachea is not exposed and other chemical reaction detection devices can be added on the microfluidic chip is a problem that technicians in this field urgently need to solve. Summary of the Invention

[0006] In view of this, the present invention provides a microfluidic chip gas control pump valve mechanism, centrifugal device, and centrifuge, in which the air pipe is not exposed and other chemical reaction detection devices can be added to the microfluidic chip.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] The present invention provides a pump-valve mechanism for gas control of a microfluidic chip, comprising:

[0009] A turntable housing, wherein a microfluidic chip is fixed on the top surface of the turntable housing, and an air nozzle A and an air nozzle B are respectively provided on the microfluidic chip for supplying and exhausting air to the inner ring air control channel and the outer ring air control channel;

[0010] An interface adapter, the interface adapter being fixed inside the turntable housing and having an A connector and a B connector, the upper end of the A connector being plugged into and connected to the A gas nozzle, and the upper end of the B connector being plugged into and connected to the B gas nozzle;

[0011] A pump-valve assembly is fixed inside the turntable housing, and two air outlets of the pump-valve assembly are respectively connected to the lower end of the A connector and the lower end of the B connector.

[0012] It can be seen from the above technical solution that compared with the prior art, the present invention discloses a pump-valve mechanism for gas control of a microfluidic chip. The pump-valve mechanism is arranged below the microfluidic chip and inside the turntable housing, so that the air pipe and pump body used for gas supply are not exposed, thereby improving the overall aesthetics and neatness of the structure. In addition, there are no other components above the microfluidic chip, so that other chemical reaction detection devices can be added above the microfluidic chip. For example, a temperature control device for providing a cooling or heating analysis environment for the microfluidic chip can be directly arranged above the microfluidic chip.

[0013] Furthermore, the turntable housing includes: a turntable bottom shell, a turntable middle shell, and a turntable surface shell. The turntable bottom shell and the turntable surface shell are respectively fixed to the bottom and top ends of the turntable middle shell, the microfluidic chip is fixed to the bottom end surface of the turntable surface shell, the interface adapter is installed on the turntable middle shell, and the pump valve assembly is installed on the top end surface of the turntable bottom shell.

[0014] The beneficial effects of adopting the above technical solution are: making the turntable housing assembleable and facilitating the construction of the pump and valve assembly.

[0015] Furthermore, the pump and valve assembly includes a positive pressure pump, a negative pressure pump, a first normally open valve, a second normally open valve, a first normally closed valve, a second normally closed valve, a third normally closed valve, a fourth normally closed valve, a first tee, a second tee, a third tee, and a fourth tee, all of which are installed on the turntable bottom shell;

[0016] The positive pressure pump is connected to the third normally closed valve via a first power line, the first normally open valve is connected to the second normally closed valve via a second power line, the negative pressure pump is connected to the fourth normally closed valve via a third power line, and the second normally open valve is connected to the first normally closed valve via a fourth power line;

[0017] The positive pressure port of the positive pressure pump is connected to the A port of the third normally closed valve via a first air pipe, the B port of the third normally closed valve is connected to the A port of the first three-way pipe via a second air pipe, the B port of the first three-way pipe is connected to the B port of the first normally closed valve via a third air pipe, the A port of the first normally closed valve is connected to the B port of the fourth three-way pipe via a fourth air pipe, and the A port of the fourth three-way pipe is in communication with the lower end of the B connector;

[0018] Port C of the first three-way pipe is connected to port B of the first normally open valve via a fifth air pipe, port A of the first normally open valve is connected to port C of the second three-way pipe via a sixth air pipe, port A of the second three-way pipe is connected to the lower end of the A connector, port B of the second three-way pipe is connected to port A of the second normally closed valve via a seventh air pipe, and port B of the second normally closed valve is connected to port B of the third three-way pipe via an eighth air pipe;

[0019] The negative pressure port of the negative pressure pump is connected to the A port of the fourth normally closed valve through the ninth air pipe, the B port of the fourth normally closed valve is connected to the A port of the third three-way pipe through the tenth air pipe, the C port of the third three-way pipe is connected to the B port of the second normally open valve through the eleventh air pipe, and the A port of the second normally open valve is connected to the C port of the fourth three-way pipe through the twelfth air pipe.

[0020] The beneficial effect of adopting the above technical solution is that it can provide four air supply modes for the air nozzle A on the inner ring air control channel and the air nozzle B on the outer ring air control channel, namely, simultaneously supplying positive pressure to the air nozzle A and the air nozzle B, simultaneously supplying negative pressure to the air nozzle A and the air nozzle B, and supplying negative pressure to the air nozzle A and the air nozzle B. This can achieve precise control of the air control valve area on the microfluidic chip, thereby controlling the deformation of the membrane structure and thus controlling the liquid flow in the spacer area. The specific principle is as follows:

[0021] The working principle of the normally closed valve is that when the power is off, the A and B ports at both ends are blocked, and when the power is on, the A and B ports at both ends are ventilated; the working principle of the normally open valve is that when the power is off, the A and B ports at both ends are ventilated, and when the power is on, the A and B ports at both ends are blocked.

[0022] The positive pressure pump and the third normally closed valve are energized or de-energized at the same time, the first normally open valve and the second normally closed valve are energized or de-energized at the same time, the negative pressure pump and the fourth normally closed valve are energized or de-energized at the same time, and the second normally open valve and the first normally closed valve are energized or de-energized at the same time.

[0023] The working principle of supplying positive pressure to air nozzles A and B at the same time is: the positive pressure pump and the third normally closed valve are energized, the second normally open valve and the first normally closed valve are energized, the first normally open valve and the second normally closed valve are not energized, and the negative pressure pump and the fourth normally closed valve are not energized. The positive-pressure gas generated by the positive-pressure pump passes through the first air pipe to the A port of the third normally closed valve. Since the third normally closed valve and the positive-pressure pump are energized at the same time, the third normally closed valve is ventilated at this time. The positive-pressure gas passes through the B port of the third normally closed valve and flows through the second air pipe to the A port of the first three-way pipe. The positive-pressure gas passes through the C port of the first three-way pipe and flows through the fifth air pipe to the B port of the first normally open valve. Since the first normally open valve is not energized, the first normally open valve is ventilated at this time. The positive-pressure gas passes through the A port of the first normally open valve and flows through the sixth air pipe to the C port of the second three-way pipe. The B port of the second three-way pipe flows through the seventh air pipe to the A port of the second normally closed valve. Since the second normally closed valve is not energized, the second normally closed valve is closed at this time. The positive-pressure gas will not flow out through the B port of the second normally closed valve. The positive-pressure gas will only come out from the A port of the second three-way pipe, enter the A joint, and then enter the A gas nozzle. In addition, the positive-pressure gas flows through the B port of the first three-way pipe and the third air pipe to the B port of the first normally closed valve. Since the first normally closed valve is energized, the first normally closed valve is ventilated at this time. The positive-pressure gas flows through the A port of the first normally closed valve and the fourth air pipe to the B port of the fourth three-way pipe. The C port of the fourth three-way pipe flows to the A port of the second normally open valve through the twelfth air pipe. Since the second normally open valve is energized, the second normally open valve is closed at this time. The positive-pressure gas will not flow through the B port of the second normally open valve. The positive-pressure gas will only come out from the A port of the fourth three-way pipe, enter the B joint, and then enter the B gas nozzle, thereby realizing the simultaneous supply of positive-pressure gas to the A gas nozzle and the B gas nozzle.

[0024] The working principle of supplying negative pressure to air nozzles A and B at the same time is: the positive pressure pump and the third normally closed valve are not energized, the second normally open valve and the first normally closed valve are not energized, the first normally open valve and the second normally closed valve are energized, and the negative pressure pump and the fourth normally closed valve are energized. The negative pressure pump generates negative pressure when it works. The negative pressure flows through the ninth air pipe to the A port of the fourth normally closed valve. Since the fourth normally closed valve is energized, the fourth normally closed valve is ventilated at this time. The negative pressure flows through the B port of the fourth normally closed valve through the tenth air pipe to the A port of the third three-way pipe. The negative pressure enters the B port of the second normally open valve through the eleventh air pipe via the C port of the third three-way pipe. Since the second normally open valve is not energized, the second normally open valve is ventilated at this time. The negative pressure flows through the A port of the second normally open valve through the twelfth air pipe to the C port of the fourth three-way pipe. The negative pressure flows through the fourth air pipe via the B port of the fourth three-way pipe to the first normally closed valve. At this time, the first normally closed valve is not energized, so the first normally closed valve is closed. The negative pressure will not flow out from the B port of the first normally closed valve. The negative pressure will only come out from the A port of the fourth three-way pipe, enter the B joint, and then enter the B air nozzle. In addition, the negative pressure flows through the B port of the third three-way pipe and the eighth air pipe to the B port of the second normally closed valve. Since the second normally closed valve is energized, the second normally closed valve is ventilated at this time. The negative pressure flows through the A port of the second normally closed valve and the seventh air pipe to the B port of the second three-way pipe. The negative pressure flows through the C port of the second three-way pipe and the sixth air pipe to the A port of the first normally open valve. Since the first normally open valve is energized, the first normally open valve is closed at this time. The negative pressure will not flow out from the B port of the first normally open valve. The negative pressure will only come out from the A port of the second three-way pipe, enter the A joint, and then enter the A nozzle, thereby realizing the simultaneous supply of negative pressure gas to the A nozzle and the B nozzle.

[0025] The working principle of supplying positive pressure to air nozzle A and negative pressure to air nozzle B is as follows: the positive pressure pump and the third normally closed valve are energized, the second normally open valve and the first normally closed valve are not energized, the first normally open valve and the second normally closed valve are not energized, and the negative pressure pump and the fourth normally closed valve are energized. The positive pressure gas generated by the positive pressure pump flows through the first air pipe to the A port of the third normally closed valve. Since the third normally closed valve is energized, the third normally closed valve is ventilated at this time. The positive pressure gas flows through the B port of the third normally closed valve through the second air pipe to the A port of the first three-way pipe. The positive pressure gas flows through the B port of the first three-way pipe through the third air pipe to the B port of the first normally closed valve. Since the first normally closed valve is not energized, the first normally closed valve is not ventilated. The positive pressure gas will not flow out from the A port of the first normally closed valve. The positive pressure gas will only ventilate through the C port of the first three-way pipe. The positive pressure gas flows through the fifth air pipe to the B port of the first normally open valve. Since the first normally open valve is not energized, the first normally open valve is ventilated at this time. The positive pressure gas flows through the A port of the first normally open valve through the sixth air pipe to the C port of the second three-way pipe. The positive pressure gas flows through the B port of the second three-way pipe to the A port of the second normally closed valve. Since the second normally closed valve is not energized, the second normally closed valve is closed at this time. The positive pressure gas will not flow out from the B port of the second normally closed valve. The positive pressure gas can only flow out from the A port of the second three-way pipe, enter the A joint, and then enter the A gas nozzle. The negative pressure pump generates negative pressure when it works. The negative pressure flows to the A port of the fourth normally closed valve through the ninth air pipe. Since the fourth normally closed valve is energized, the fourth normally closed valve is ventilated at this time. The negative pressure flows through the B port of the fourth normally closed valve through the tenth air pipe to the A port of the third three-way pipe. The negative pressure flows through the B port of the third three-way pipe through the eighth air pipe to the B port of the second normally closed valve. Since the second normally closed valve is not energized, the second normally open valve is not ventilated at this time, and the negative pressure will not flow out from the A port of the second normally open valve. The negative pressure is ventilated through the C port of the third three-way pipe. The negative pressure flows through the eleventh air pipe to the B port of the second normally open valve. Because the second normally open valve is not energized, the second normally open valve is ventilated at this time. The negative pressure comes out from the A port of the second normally open valve and enters the C port of the fourth three-way pipe. The negative pressure flows through the B port of the fourth three-way pipe and flows to the A port of the first normally closed valve through the fourth air pipe. Because the first normally closed valve is not energized, the first normally closed valve is not ventilated. The negative pressure can only come out from the A port of the fourth three-way pipe, enter the B joint, and then enter the B air nozzle, thereby supplying positive pressure to the A air nozzle and negative pressure to the B air nozzle.

[0026] The working principle of supplying negative pressure to air nozzle A and positive pressure to air nozzle B is as follows: the positive pressure pump and the third normally closed valve are energized, the second normally open valve and the first normally closed valve are energized, the first normally open valve and the second normally closed valve are energized, and the negative pressure pump and the fourth normally closed valve are energized. The positive pressure pump generates positive pressure gas, which flows through the first air pipe to the A port of the third normally closed valve. Since the third normally closed valve is energized, the third normally closed valve is ventilated at this time. The positive pressure gas flows through the B port of the third normally closed valve through the second air pipe to the A port of the first three-way pipe. The positive pressure gas flows through the C port of the first three-way pipe through the fifth air pipe to the B port of the first normally open valve. Since the first normally open valve is energized, it does not conduct gas, so the positive pressure gas will not flow out from the A port of the first normally open valve. The positive pressure gas can only pass through the B port of the first three-way pipe. The positive pressure gas flows through the third air pipe to the B port of the first normally closed valve. Since the first normally closed valve is energized, the first normally closed valve is ventilated at this time. The positive pressure gas flows through the A port of the first normally closed valve through the fourth air pipe to the B port of the fourth three-way pipe. The positive pressure gas flows through the C port of the fourth three-way pipe through the twelfth air pipe to the A port of the second normally open valve. Since the second normally open valve is energized, the second normally open valve is closed at this time. The positive pressure gas will not flow out from the B port of the second normally open valve. The positive pressure gas can only flow out from the A port of the fourth three-way pipe, enter the B joint, and then enter the B gas nozzle. The negative pressure pump generates negative pressure when it works. The negative pressure flows through the ninth air pipe to the A port of the fourth normally closed valve. Since the fourth normally closed valve is energized, the fourth normally closed valve is ventilated at this time. The negative pressure flows through the B port of the fourth normally closed valve through the tenth air pipe to the A port of the third three-way pipe. The negative pressure flows through the C port of the third three-way pipe through the eleventh air pipe to the B port of the second normally open valve. Since the second normally open valve is energized, the second normally open valve is not ventilated at this time, and the negative pressure will not flow out from the A port of the second normally open valve. Negative pressure can only flow through the B port of the third tee through the eighth air pipe to the B port of the second normally closed valve. Because the second normally closed valve is energized, the second normally closed valve is ventilated at this time. The negative pressure flows through the A port of the second normally closed valve through the seventh air pipe to the B port connected to the second tee. The negative pressure flows through the C port of the second tee through the sixth air pipe to the A port of the first normally open valve. Because the first normally open valve is energized, the first normally open valve is closed at this time, and the negative pressure will not flow out from the B port of the first normally open valve. The negative pressure can only flow out from the A port of the second tee into the A joint and then into the A nozzle, thereby supplying negative pressure to the A gas and positive pressure to the B nozzle.

[0027] The present invention provides a centrifugal device, comprising: the pump-valve mechanism and a centrifugal drive unit, wherein a driving spindle of the centrifugal drive unit is fixedly connected to the pump-valve mechanism.

[0028] The beneficial effect of adopting the above technical solution is: driving the flow of liquid in the microfluidic chip by centrifugal force.

[0029] Furthermore, the centrifugal drive unit includes:

[0030] A centrifugal motor protection bracket, wherein a centrifugal motor is arranged inside the centrifugal motor protection bracket;

[0031] A spindle bracket, wherein the bottom end of the spindle bracket is fixedly connected to the top of the centrifugal motor protection bracket, the spindle bracket is fixedly connected to the top of the centrifugal motor housing, a bearing is fixed to the top of the spindle bracket, the driving spindle of the centrifugal motor passes upward through the spindle bracket and is fixed to the inner ring of the bearing, and the connecting boss on the driving spindle is fixedly connected to the turntable bottom shell;

[0032] An electric slip ring, wherein the inner cylinder of the electric slip ring is fixedly sleeved on the driving main shaft located in the main shaft support section, the outer cylinder of the electric slip ring is fixedly connected to the main shaft support via an outer cylinder fixing frame, and the wires inside the inner cylinder of the electric slip ring are electrically connected to the positive pressure pump, the negative pressure pump, the first normally open valve, the second normally open valve, the first normally closed valve, the second normally closed valve, the third normally closed valve, and the fourth normally closed valve, respectively.

[0033] The beneficial effect of adopting the above technical solution is that the provision of the electric slip ring can realize the connection between the power line and the positive-pressure pump, the negative-pressure pump, the first normally open valve, the second normally open valve, the first normally closed valve, the second normally closed valve, the third normally closed valve, and the fourth normally closed valve without causing the problem of winding.

[0034] Furthermore, the centrifugal drive unit also includes a centrifugal protective shell covering the outer peripheral side of the turntable housing, and the bottom shell of the centrifugal protective shell is fixedly connected to the spindle bracket.

[0035] The beneficial effects of the above technical solution are: preventing the microfluidic chip from being thrown out by centrifugal force and injuring people, and also preventing the fluid on the microfluidic chip from splashing outward onto people and causing danger. Therefore, the centrifugal protective housing improves the safety of the device.

[0036] The present invention provides a centrifuge, comprising:

[0037] counterweight base plate;

[0038] The centrifugal device, the centrifugal motor protection bracket is fixed to the counterweight base plate;

[0039] A temperature control mechanism is installed on the counterweight base plate and is used to provide a heating or cooling analysis environment for the microfluidic chip.

[0040] Furthermore, a first centrifugal mounting hole is provided on the counterweight bottom plate, and the temperature control mechanism includes:

[0041] A guide rail supporting base plate, wherein the guide rail supporting base plate is fixed to the top of the counterweight base plate, a second centrifugal mounting hole is opened at a position on the guide rail supporting base plate corresponding to the first centrifugal mounting hole, and the centrifugal motor protection bracket is fixed to the edge of the second centrifugal mounting hole through the mounting ear plate thereon;

[0042] Guide rail supporting side plates, wherein the guide rail supporting side plates are fixed at intervals on both sides of the guide rail supporting bottom plate, the centrifugal device is located between the two guide rail supporting side plates, and linear guide rails are installed on the opposite side walls of the two guide rail supporting side plates;

[0043] A temperature-controlled cover translation mechanism, wherein the translation connecting plates on both side walls of the temperature-controlled cover translation mechanism are fixedly connected to the sliders on the two linear slide rails respectively;

[0044] A translation drive mechanism, wherein the translation drive mechanism is fixed to one of the guide rail support side plates, and the translation portion of the translation drive mechanism is fixed to one of the translation connecting plates, and is used to drive the temperature control cover translation mechanism to translate to above the microfluidic chip to provide a heating or cooling analysis environment for the microfluidic chip.

[0045] The beneficial effect of adopting the above technical solution is: when working, the microfluidic chip is placed on the turntable surface shell, and then the translation drive mechanism drives the temperature control cover translation mechanism to move horizontally to the top of the microfluidic chip, and then the centrifugal motor drives the microfluidic chip to rotate, and the temperature control cover translation mechanism provides the microfluidic chip with a heating or cooling analysis environment, ensuring the working environment required for sample analysis.

[0046] Furthermore, the temperature control cover translation mechanism includes:

[0047] The upper shell fixed module comprises:

[0048] An upper shell, wherein the translation connecting plates are fixed on both side walls of the upper shell, and a plurality of guide rods are evenly fixed on the inner top wall of the upper shell;

[0049] A screw lifting motor, wherein the housing of the screw lifting motor is fixed to the top of the upper shell, and the screw of the screw lifting motor extends downward through the upper shell;

[0050] The lower shell moving module comprises:

[0051] A connecting disk, wherein a screw rod passing hole is opened in the middle of the connecting disk, a screw rod nut is fixed on the screw rod passing hole, the screw rod nut is threadedly connected to the screw rod, and a plurality of guide cylinders are evenly fixed on the top of the connecting disk, and the plurality of guide rods are plugged into the plurality of guide cylinders;

[0052] A cooling plate, the cooling plate being fixed at intervals on the bottom end surface of the connecting plate;

[0053] A cooling water pipe is provided between the connecting plate and the cooling plate, and the cooling water pipe plate is fixed on the top of the cooling plate, and both ends of the cooling water pipe are respectively connected to the water inlet and outlet of the external cooling host;

[0054] A heating block is fixed on the bottom surface of the connecting plate, a PI heating film is fixed on the top surface of the heating block, and a temperature sensor for monitoring its temperature is installed on the PI heating film.

[0055] The beneficial effects of adopting the above technical solution are: the screw lifting motor drives the lower shell moving module to descend, so that the cooling plate and the heating block are close to the microfluidic chip. When a cooling analysis environment is required, circulating cooling water is introduced into the cooling water pipe, and a cooling analysis environment is provided for the analysis of the microfluidic chip through the cooling plate. When a heating analysis environment is required, the PI heating film is energized to generate heat, which passes through the heating block to provide a heating analysis environment for the analysis of the microfluidic chip.

[0056] Furthermore, one of the guide rail support side plates is provided with an elongated hole, a lower clamping block is fixed to one of the translation connecting plates, an upper clamping block is fixed to the lower clamping block, and the lower clamping block is arranged through the elongated hole, and the translation driving mechanism includes:

[0057] a translation motor, the translation motor being fixed on a side wall of one of the guide rail supporting side plates;

[0058] A driving pulley, wherein the driving pulley shaft is rotatably mounted on the other side wall of one of the guide rail supporting side plates, and the driving pulley shaft is fixedly connected to the driving shaft of the translation motor;

[0059] A driven pulley, wherein the driven pulley shaft of the driven pulley is rotatably mounted on the other side wall of one of the guide rail supporting side plates;

[0060] A translational synchronous belt, wherein the translational synchronous belt is sleeved on the driving pulley and the driven pulley, and the upper belt of the translational synchronous belt is clamped between the lower clamping block and the upper clamping block by bolts;

[0061] A drag chain, wherein the fixed end of the drag chain is fixed to the top end of one of the guide rail support side plates, and the free end of the drag chain is fixedly connected to one of the translation connecting plates through a connecting piece.

[0062] The beneficial effect of adopting the above technical solution is: the translation motor drives the translation synchronous belt to move, and then the upper belt of the translation synchronous belt drives the temperature control cover translation mechanism to translate, thereby realizing the opening and closing of the temperature control cover translation mechanism from above the microfluidic chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0063] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0064] Figure 1 This is a structural schematic diagram of the existing gas supply and extraction device provided by the present invention.

[0065] Figure 2 This is a schematic diagram of the overall assembly structure of a pump and valve mechanism for gas control of a microfluidic chip provided by the present invention.

[0066] Figure 3 for Figure 2 Schematic diagram of the decomposition structure.

[0067] Figure 4 This is a schematic diagram of the structure of the pump and valve assembly installed on the turntable bottom shell.

[0068] Figure 5 Schematic diagram of the gas connection structure of the pump valve assembly.

[0069] Figure 6 Schematic diagram of the circuit structure of the pump valve assembly.

[0070] Figure 7 This is a schematic structural diagram of a centrifugal device provided by the present invention.

[0071] Figure 8 for Figure 7 Schematic diagram of the main cross-section structure.

[0072] Figure 9 for Figure 7 Schematic diagram of the main cross-section structure.

[0073] Figure 10 This is a structural schematic diagram of a centrifuge provided by the present invention from a first perspective.

[0074] Figure 11 This is a structural schematic diagram of a centrifuge provided by the present invention from a second perspective.

[0075] Figure 12 This is a schematic diagram of the exploded structure of the guide rail support base plate and the guide rail support side plate.

[0076] Figure 13 It is a schematic diagram of the axial structure of the temperature control cover translation mechanism.

[0077] Figure 14 for Figure 13 Schematic diagram of the main cross-section structure.

[0078] Figure 15 for Figure 13 Schematic diagram of the decomposition structure.

[0079] Figure 16 It is a structural diagram of the upper shell fixed module.

[0080] Figure 17 This is a structural diagram of the lower shell dynamic module from the first perspective.

[0081] Figure 18 This is a structural diagram of the lower shell dynamic module from a second perspective.

[0082] Figure 19 This is a schematic diagram of an existing microfluidic chip provided by the present invention. DETAILED DESCRIPTION

[0083] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0084] like Figure 2-Figure 6 As shown, an embodiment of the present invention discloses a pump-valve mechanism for air control of a microfluidic chip, comprising: a turntable housing 1, a microfluidic chip 400 is fixed on the top surface of the turntable housing 1, and the microfluidic chip 400 is respectively provided with an A air nozzle 4001 and a B air nozzle 4002 for supplying air to and exhausting air from the inner ring air control channel and the outer ring air control channel; an interface adapter 2, the interface adapter 2 is fixed inside the turntable housing 1, and has an A connector 201 and a B connector 202, the upper end of the A connector 201 is plugged into and connected with the A air nozzle 4001, and the upper end of the B connector 202 is plugged into and connected with the B air nozzle 4002; a pump-valve assembly 3, the pump-valve assembly 3 is fixed inside the turntable housing 1, and the two air outlets of the pump-valve assembly 3 are respectively connected to the lower end of the A connector 201 and the lower end of the B connector 202.

[0085] Among them, the turntable shell 1 includes: a turntable bottom shell 101, a turntable middle shell 102, and a turntable surface shell 103. The turntable bottom shell 101 and the turntable surface shell 103 are respectively fixed on the bottom and top ends of the turntable middle shell 102. The microfluidic chip 400 is fixed on the bottom end surface of the turntable surface shell 103. The interface adapter 2 is installed on the turntable middle shell 102. The pump valve assembly 3 is installed on the top end surface of the turntable bottom shell 101.

[0086] See Figure 5 and Figure 6The pump-valve assembly 3 includes a positive-pressure pump 301, a negative-pressure pump 302, a first normally-open valve 303, a second normally-open valve 304, a first normally-closed valve 305, a second normally-closed valve 306, a third normally-closed valve 307, a fourth normally-closed valve 308, a first three-way pipe 309, a second three-way pipe 310, a third three-way pipe 311, and a fourth three-way pipe 312, all of which are installed on the turntable bottom shell 101; the positive-pressure pump 301 is connected to the third normally-closed valve 307 via a first power line 313, the first normally-open valve 303 is connected to the second normally-closed valve 306 via a second power line 314, the negative-pressure pump 302 is connected to the fourth normally-closed valve 308 via a third power line 315, and the second normally-open valve 304 is connected to the first normally-closed valve 305 via a fourth power line 316;

[0087] The positive pressure port of the positive pressure pump 301 is connected to the A port of the third normally closed valve 307 through the first air pipe 317, the B port of the third normally closed valve 307 is connected to the A port of the first three-way pipe 309 through the second air pipe 318, the B port of the first three-way pipe 309 is connected to the B port of the first normally closed valve 305 through the third air pipe 319, the A port of the first normally closed valve 305 is connected to the B port of the fourth three-way pipe 312 through the fourth air pipe 320, and the A port of the fourth three-way pipe 312 is connected to the lower end of the B connector 202; the C port of the first three-way pipe 309 is connected to the B port of the first normally open valve 303 through the fifth air pipe 321, the A port of the first normally open valve 303 is connected to the C port of the second three-way pipe 310 through the sixth air pipe 322, and the The A port of the second three-way pipe 310 is connected to the lower end of the A connector 201, the B port of the second three-way pipe 310 is connected to the A port of the second normally closed valve 306 through the seventh air pipe 323, and the B port of the second normally closed valve 306 is connected to the B port of the third three-way pipe 311 through the eighth air pipe 324; the negative pressure port of the negative pressure pump 302 is connected to the A port of the fourth normally closed valve 308 through the ninth air pipe 325, the B port of the fourth normally closed valve 308 is connected to the A port of the third three-way pipe 311 through the tenth air pipe 326, the C port of the third three-way pipe 311 is connected to the B port of the second normally open valve 304 through the eleventh air pipe 327, and the A port of the second normally open valve 304 is connected to the C port of the fourth three-way pipe 312 through the twelfth air pipe 328.

[0088] like Figure 7-Figure 9 As shown, the present invention provides a centrifugal device, comprising: the above-mentioned pump-valve mechanism and a centrifugal driving part 4, wherein the driving main shaft of the centrifugal driving part 4 is fixedly connected to the pump-valve mechanism.

[0089] Among them, the centrifugal drive unit 4 includes: a centrifugal motor protection bracket 401, a centrifugal motor 402 is arranged inside the centrifugal motor protection bracket 401; a spindle bracket 403, the bottom end of the spindle bracket 403 is fixedly connected to the top of the centrifugal motor protection bracket 401, the spindle bracket 403 is fixedly connected to the top of the shell of the centrifugal motor 402, a bearing 404 is fixed on the top of the spindle bracket 403, a driving spindle 4021 of the centrifugal motor 402 passes upward through the spindle bracket 403 and is fixed to the inner ring of the bearing 404, and the connecting cam on the driving spindle 4021 The platform 40211 is fixedly connected to the turntable bottom shell 101; the electric slip ring 405, the inner cylinder of the electric slip ring 405 is fixedly sleeved on the driving main shaft 4021 located in the main shaft support 403 section, the outer cylinder of the electric slip ring 405 is fixedly connected to the main shaft support 403 via the outer cylinder fixing frame 406, and the wires inside the inner cylinder of the electric slip ring 405 are electrically connected to the positive pressure pump 301, the negative pressure pump 302, the first normally open valve 303, the second normally open valve 304, the first normally closed valve 305, the second normally closed valve 306, the third normally closed valve 307, and the fourth normally closed valve 308 respectively.

[0090] The centrifugal drive unit 4 further includes a centrifugal protective shell 407 covering the outer circumference of the turntable housing 1 , and a bottom shell of the centrifugal protective shell 407 is fixedly connected to the spindle support 403 .

[0091] like Figures 10-18 As shown, the present invention provides a centrifuge, comprising: a counterweight base plate 5; the above-mentioned centrifugal device, a centrifugal motor protection bracket 401 is fixed on the counterweight base plate 5; a temperature control mechanism 6, the temperature control mechanism 6 is installed on the counterweight base plate 5, and is used to provide a heating or cooling analysis environment for the microfluidic chip 400.

[0092] Among them, a first centrifugal mounting hole is provided on the counterweight base plate 5, and the temperature control mechanism 6 includes: a guide rail supporting base plate 601, the guide rail supporting base plate 601 is fixed on the top of the counterweight base plate 5, a second centrifugal mounting hole 6011 is provided on the guide rail supporting base plate 601 at a position corresponding to the first centrifugal mounting hole, and the centrifugal motor protection bracket 401 is fixed on the edge of the second centrifugal mounting hole 6011 through the mounting ear plate 4011 thereon; a guide rail supporting side plate 602, the guide rail supporting side plate 602 is two fixed at intervals on both sides of the guide rail supporting base plate 601, and the centrifugal device is located between the two guide rail supporting side plates 602. Linear slide rails 603 are installed on the opposite side walls of the support side plates 602; the temperature control cover translation mechanism 604, the translation connecting plates 60412 on the two side walls of the temperature control cover translation mechanism 604 are respectively fixedly connected to the sliders on the two linear slide rails 603; the translation drive mechanism 605, the translation drive mechanism 605 is fixed on one of the guide rail supporting side plates 602, and the translation part of the translation drive mechanism 605 is fixed to one of the translation connecting plates 60412, which is used to drive the temperature control cover translation mechanism 604 to translate to the top of the microfluidic chip 400 to provide a heating or cooling analysis environment for the microfluidic chip 400.

[0093] Specifically, the temperature control cover translation mechanism 604 includes: an upper shell fixed module 6041, which includes: an upper shell 60411, on both sides of which are fixed translation connecting plates 60412, and on the inner top wall of the upper shell 60411 are evenly fixed multiple guide rods 60413; a screw lifting motor 60414, the housing of which is fixed to the upper shell 60411. At the top, the screw 604141 of the screw lifting motor 60414 extends downward through the upper shell 60411; the lower shell movable module 6042, the lower shell movable module 6042 includes: a connecting disk 60421, a screw rod through hole 604211 is opened in the middle of the connecting disk 60421, a screw rod nut 60422 is fixed on the screw rod through hole 604211, and the screw rod nut 60422 is threadedly connected to the screw rod 604141 , multiple guide cylinders 60423 are evenly fixed on the top of the connecting disk 60421, and multiple guide rods 60413 are plugged into the multiple guide cylinders 60423; the cooling disk 60424, the cooling disk 60424 is fixed at intervals on the bottom end surface of the connecting disk 60421; the cooling water pipe 60425, the cooling water pipe 60425 is arranged between the connecting disk 60421 and the cooling disk 60424, and the cooling water pipe 60425 is fixed on the top of the cooling disk 60424, and the two ends of the cooling water pipe 60425 are respectively connected to the inlet and outlet water circulation ports of the external cooling host 7; the heating block 60426, the heating block 60426 is fixed on the bottom end surface of the connecting disk 60421, and a PI heating film 60427 is fixed on the top surface of the heating block 60426, and a temperature sensor 60428 for monitoring its temperature is installed on the PI heating film 60427.

[0094] A photomultiplier tube ( Figure 15 It is indicated but not labeled in the figure) and is used for chemiluminescence detection to monitor whether the chemical reaction data is normal.

[0095] One of the guide rail supporting side plates 602 is provided with an elongated hole 6021, one of the translation connecting plates 60412 is fixed with a lower clamping block 8, the lower clamping block 8 is fixed with an upper clamping block 9, and the lower clamping block 8 is arranged through the elongated hole 6021. The translation driving mechanism 605 includes: a translation motor 6051, which is fixed to one side wall of one of the guide rail supporting side plates 602; a driving pulley 6052, the driving wheel shaft of which is rotatably mounted on the other side wall of one of the guide rail supporting side plates 602, and the driving wheel shaft is fixedly connected to the driving shaft of the translation motor 6051. Connect; driven pulley 6053, the driven pulley shaft of the driven pulley 6053 is rotatably mounted on the other side wall of one of the guide rail supporting side plates 602; translational synchronous belt 6054, the translational synchronous belt 6054 is sleeved on the driving pulley 6052 and the driven pulley 6053, and the upper belt 60541 of the translational synchronous belt 6054 is clamped between the lower clamping block 8 and the upper clamping block 9 by bolts; drag chain 6055, the fixed end of the drag chain 6055 is fixed to the top of one of the guide rail supporting side plates 602, and the free end of the drag chain 6055 is fixedly connected to one of the translation connecting plates 60412 through a connector 6056.

[0096] See also Figure 19 The microfluidic chip 400 (external structure) of the present invention is equipped with a sample loading reservoir R0, a reaction reservoir R1, a reaction reservoir R2, a reaction reservoir R3, and a waste liquid reservoir R4 from the inside out. The spacers between reaction reservoirs R1 and R2, and between reaction reservoirs R3 and waste liquid reservoir R4, correspond to the air-controlled valve area of ​​the inner air-controlled channel L1, while the spacers between reaction reservoirs R2 and R3 correspond to the air-controlled valve area of ​​the outer air-controlled channel L2. Liquid flow in these spacers is controlled by controlling the deformation of the membrane assembly (an elastic membrane) in the air-controlled valve area. When the inner air-controlled channel L1 or the outer air-controlled channel L2 is inflated, the elastic membrane positively deforms (expands), sealing the gaps between the reaction wells and preventing liquid transfer between the wells. When the air is evacuated, the elastic membrane negatively deforms (contracts), opening the gaps between the reaction wells and facilitating liquid transfer between the wells. Furthermore, a gas nozzle A 4001 is provided on the inner air-controlled channel L1, and a gas nozzle B 4002 is provided on the outer air-controlled channel L2.

[0097] The pump-valve assembly 3 of the present invention can provide four air supply modes to the A nozzle 4001 on the inner air control channel L1 and the B nozzle 4002 on the outer air control channel L2: simultaneously supplying positive pressure to both nozzles A and B, simultaneously supplying negative pressure to both nozzles A and B, supplying positive pressure to nozzle A and negative pressure to nozzle B, or supplying negative pressure to nozzle A and positive pressure to nozzle B. This enables precise control of the air control valve area on the microfluidic chip, thereby controlling the deformation of the membrane structure and thus the flow of liquid in the spacer area. The pump-valve assembly 3 also implements a pressure maintenance function (the pump only needs to operate for 3 seconds to maintain pressure).

[0098] The specific principles are as follows:

[0099] The normally closed valve operates on the principle that when de-energized, ports A and B at both ends are closed, and when energized, ports A and B are open. The normally open valve operates on the principle that when de-energized, ports A and B at both ends are open, and when energized, ports A and B at both ends are closed. The positive-pressure pump and the third normally closed valve are energized or de-energized simultaneously, the first normally open valve and the second normally closed valve are energized or de-energized simultaneously, the negative-pressure pump and the fourth normally closed valve are energized or de-energized simultaneously, and the second normally open valve and the first normally closed valve are energized or de-energized simultaneously.

[0100] The working principle of supplying positive pressure to the A nozzle 4001 and the B nozzle 4002 at the same time is as follows: the positive pressure pump 301 and the third normally closed valve 307 are energized, the second normally open valve 304 and the first normally closed valve 305 are energized, the first normally open valve 303 and the second normally closed valve 306 are not energized, and the negative pressure pump 302 and the fourth normally closed valve 308 are not energized. The positive pressure pump 301 generates positive pressure gas, which passes through the first air pipe 317 to the A port of the third normally closed valve 307. Since the third normally closed valve 307 and the positive pressure pump 301 are energized at the same time, the third normally closed valve 307 is ventilated at this time. The positive pressure gas passes through the B port of the third normally closed valve 307 through the second air pipe 318 to the A port of the first three-way pipe 309. The positive pressure gas passes through the C port of the first three-way pipe 309 through the fifth air pipe 321 to the B port of the first normally open valve 303. Since the first normally open valve 303 is not energized, at this time the first The normally open valve 303 is ventilated, and the positive pressure gas flows through the A port of the first normally open valve 303 through the sixth air pipe 322 to the C port of the second three-way pipe 310, and the B port of the second three-way pipe 310 flows through the seventh air pipe 323 to the A port of the second normally closed valve 306. Because the second normally closed valve 306 is not energized, the second normally closed valve 306 is closed at this time, and the positive pressure gas will not flow out through the B port of the second normally closed valve 306. The positive pressure gas will only come out from the A port of the second three-way pipe 310, enter the A connector 201, and then enter the A gas nozzle 4001. In addition, the positive-pressure gas flows through the B port of the first three-way pipe 309 and the third air pipe 319 to the B port of the first normally closed valve 305. Since the first normally closed valve 305 is energized, the first normally closed valve 305 is ventilated at this time. The positive-pressure gas flows through the A port of the first normally closed valve 305 and the fourth air pipe 320 to the B port of the fourth three-way pipe 312. The C port of the fourth three-way pipe 312 flows through the twelfth air pipe 328 to the A port of the second normally open valve 304. Since the second normally open valve 304 is energized, the second normally open valve 304 is closed at this time. The positive-pressure gas will not flow through the B port of the second normally open valve 304. The positive-pressure gas will only come out from the A port of the fourth three-way pipe 312, enter the B connector 202, and then enter the B gas nozzle 4002, thereby realizing the simultaneous supply of positive-pressure gas by the A gas nozzle 4001 and the B gas nozzle 4002.

[0101] The working principle of supplying negative pressure to the A nozzle 4001 and the B nozzle 4002 at the same time is as follows: the positive pressure pump 301 and the third normally closed valve 307 are de-energized, the second normally open valve 304 and the first normally closed valve 305 are de-energized, the first normally open valve 303 and the second normally closed valve 306 are energized, and the negative pressure pump 302 and the fourth normally closed valve 308 are energized. The negative pressure pump 302 generates negative pressure, which flows through the ninth air pipe 325 to the A port of the fourth normally closed valve 308. Since the fourth normally closed valve 308 is energized, the fourth normally closed valve 308 is now ventilated. The negative pressure flows through the B port of the fourth normally closed valve 308 through the tenth air pipe 326 to the A port of the third three-way pipe 311. The negative pressure enters the B port of the second normally open valve 304 through the eleventh air pipe 327 via the C port of the third three-way pipe 311. Since the second normally open valve 304 is de-energized, the second normally open valve 304 is ventilated. At this time, the air is ventilated, and the negative pressure flows through the A port of the second normally open valve 304 through the twelfth air pipe 328 to the C port of the fourth three-way pipe 312. The negative pressure flows through the B port of the fourth three-way pipe 312 through the fourth air pipe 320 to the first normally closed valve 305. At this time, the first normally closed valve 305 is not energized, so the first normally closed valve 305 is closed, and the negative pressure will not flow out from the B port of the first normally closed valve 305. The negative pressure will only flow out from the A port of the fourth three-way pipe 312, enter the B connector 202, and then enter the B air nozzle 4002. In addition, the negative pressure flows through the B port of the third three-way pipe 311 and the eighth air pipe 324 to the B port of the second normally closed valve 306. Since the second normally closed valve 306 is energized, the second normally closed valve 306 is ventilated at this time. The negative pressure flows through the A port of the second normally closed valve 306 and the seventh air pipe 323 to the B port of the second three-way pipe 310. The negative pressure flows through the C port of the second three-way pipe 310 and the sixth air pipe 322 to the A port of the first normally open valve 303. Since the first normally open valve 303 is energized, the first normally open valve 303 is closed at this time, and the negative pressure will not flow out from the B port of the first normally open valve 303. The negative pressure will only come out from the A port of the second three-way pipe 310, enter the A connector 201, and then enter the A gas nozzle 4001, thereby realizing the simultaneous supply of negative pressure gas to the A gas nozzle 4001 and the B gas nozzle 4002.

[0102] The working principle of supplying positive pressure to the A nozzle 4001 and negative pressure to the B nozzle 4002 is as follows: the positive pressure pump 301 and the third normally closed valve 307 are energized, the second normally open valve 304 and the first normally closed valve 305 are not energized, the first normally open valve 303 and the second normally closed valve 306 are not energized, and the negative pressure pump 302 and the fourth normally closed valve 308 are energized. The positive pressure gas generated by the positive pressure pump 301 flows through the first air pipe 317 to the A port of the third normally closed valve 307. Since the third normally closed valve 307 is energized, the third normally closed valve 307 is ventilated at this time. The positive pressure gas flows through the B port of the third normally closed valve 307 through the second air pipe 318 to the A port of the first three-way pipe 309. The positive pressure gas flows through the B port of the first three-way pipe 309 through the third air pipe 319 to the B port of the first normally closed valve 305. Since the first normally closed valve 305 is not energized, the first normally closed valve 305 is not ventilated, and the positive pressure gas will not flow out from the A port of the first normally closed valve 305. The positive pressure gas only passes through the C port of the first three-way pipe 309. The fifth air pipe 324 flows to the B port of the first normally open valve 303. Since the first normally open valve 303 is not energized, the first normally open valve 303 is ventilated at this time. The positive pressure gas flows through the A port of the first normally open valve 303 and the sixth air pipe 322 to the C port of the second three-way pipe 310. The positive pressure gas flows through the B port of the second three-way pipe 310 to the A port of the second normally closed valve 306. Since the second normally closed valve 306 is not energized, the second normally closed valve 306 is closed at this time. The positive pressure gas will not flow out from the B port of the second normally closed valve 306. The positive pressure gas can only flow out from the A port of the second three-way pipe 310, enter the A connector 201, and then enter the A gas nozzle 4001. The negative pressure pump 302 generates negative pressure when it works. The negative pressure flows to the A port of the fourth normally closed valve 308 through the ninth air pipe 325. Since the fourth normally closed valve 308 is energized, the fourth normally closed valve 308 is ventilated at this time. The negative pressure flows through the B port of the fourth normally closed valve 308 through the tenth air pipe 326 to the A port of the third three-way pipe 311. The negative pressure flows through the B port of the third three-way pipe 311 through the eighth air pipe 324 to the B port of the second normally closed valve 306. Since the second normally closed valve 306 is not energized, the second normally closed valve 306 is not ventilated at this time, and the negative pressure will not flow out from the A port of the second normally closed valve 306. The negative pressure only flows through the C port of the third three-way pipe 311 through the tenth air pipe 326. An air pipe 327 flows to the B port of the second normally open valve 304. Since the second normally open valve 304 is not energized, the second normally open valve 304 is ventilated at this time. The negative pressure comes out from the A port of the second normally open valve 304 and enters the C port of the fourth three-way pipe 312. The negative pressure flows through the B port of the fourth three-way pipe 312 and the fourth air pipe 320 to the A port of the first normally closed valve 305. Since the first normally closed valve 305 is not energized, the first normally closed valve 305 is not ventilated. The negative pressure can only come out from the A port of the fourth three-way pipe 312 and enter the B connector 202 and then enter the B air nozzle 4002, thereby supplying positive pressure to the A air nozzle 4001 and negative pressure to the B air nozzle 4002.

[0103] The working principle of supplying negative pressure to the A nozzle 4001 and positive pressure to the B nozzle 4002 is as follows: the positive pressure pump 301 and the third normally closed valve 307 are energized, the second normally open valve 304 and the first normally closed valve 305 are energized, the first normally open valve 303 and the second normally closed valve 306 are energized, and the negative pressure pump 302 and the fourth normally closed valve 308 are energized. The positive pressure pump 301 generates positive pressure gas when it works. The positive pressure gas flows through the first air pipe 317 to the A port of the third normally closed valve 307. Since the third normally closed valve 307 is energized, the third normally closed valve 307 is ventilated at this time. The positive pressure gas flows through the B port of the third normally closed valve 307 through the second air pipe 318 to the A port of the first three-way pipe 309. The positive pressure gas flows through the C port of the first three-way pipe 309 through the fifth air pipe 321 to the B port of the first normally open valve 303. Since the first normally open valve 303 is energized, it does not conduct gas. Therefore, the positive pressure gas will not flow out from the A port of the first normally open valve 303. The positive pressure gas can only flow through the B port of the first three-way pipe 309 through the third normally closed valve 307. The third air pipe 319 flows to the B port of the first normally closed valve 305. Since the first normally closed valve 305 is energized, the first normally closed valve 305 is ventilated at this time. The positive pressure gas passes through the A port of the first normally closed valve 305 and the fourth air pipe 320 to the B port of the fourth three-way pipe 312. The positive pressure gas passes through the C port of the fourth three-way pipe 312 and the twelfth air pipe 328 to the A port of the second normally open valve 304. Since the second normally open valve 304 is energized, the second normally open valve 304 is closed at this time. The positive pressure gas will not flow out from the B port of the second normally open valve 304. The positive pressure gas can only flow out from the A port of the fourth three-way pipe 312, enter the B joint, and then enter the B gas nozzle 4002. The negative pressure pump 302 generates negative pressure when it works, and the negative pressure flows to the A port of the fourth normally closed valve 308 through the ninth air pipe 325. Since the fourth normally closed valve 308 is energized, the fourth normally closed valve 308 is ventilated at this time. The negative pressure flows through the B port of the fourth normally closed valve 308 through the tenth air pipe 326 to the A port of the third three-way pipe 311. The negative pressure flows through the C port of the third three-way pipe through the eleventh air pipe 321 to the B port of the second normally open valve 304. Since the second normally open valve 304 is energized, the second normally open valve 304 is not ventilated at this time, and the negative pressure will not flow out from the A port of the second normally open valve 304. Negative pressure can only flow through the B port of the third three-way pipe 311 and the eighth air pipe 324 to the B port of the second normally closed valve 306. Because the second normally closed valve 306 is energized, the second normally closed valve 306 is ventilated at this time. The negative pressure flows through the A port of the second normally closed valve 306 and the seventh air pipe 323 to the B port connected to the second three-way pipe 310. The negative pressure flows through the C port of the second three-way pipe 310 and the sixth air pipe 322 to the A port of the first normally open valve 303. Because the first normally open valve 303 is energized, the first normally open valve 303 is closed at this time, and the negative pressure will not flow out from the B port of the first normally open valve 303. The negative pressure can only flow out from the A port of the second three-way pipe 310, enter the A connector, and then enter the A gas nozzle 4001, thereby supplying negative pressure to the A gas nozzle 4001 and positive pressure to the B gas nozzle 4002.

[0104] Therefore, in Figure 19 In the process, by adding sample to the sample pool R0, and then supplying positive pressure to the A nozzle 4001 and negative pressure to the B nozzle 4002, the inner circle air control channel L1 inputs positive pressure to deform the elastic membrane to form a normally closed channel, and the outer circle air control channel L2 inputs negative pressure to deform the elastic membrane to form a normally open channel, which can achieve precise transfer of the fluid in the sample pool R0 to the reaction pool R1.

[0105] Supply negative pressure to air nozzle A and positive pressure to air nozzle B. Negative pressure is input to the inner ring air control channel L1 to deform the elastic membrane to form a normally open channel. Positive pressure is input to the outer ring air control channel L2 to deform the elastic membrane to form a normally closed channel, which can realize the precise transfer of fluid from reaction pool R1 to reaction pool R2.

[0106] Supply positive pressure to air nozzle A and negative pressure to air nozzle B. Positive pressure is input to the inner ring air control channel L1 to deform the elastic membrane to form a normally closed channel. Negative pressure is input to the outer ring air control channel L2 to deform the elastic membrane to form a normally open channel, which can achieve precise transfer of fluid from R2 to R3.

[0107] Positive pressure is supplied to air nozzles A and B, and positive pressure is input to the inner circle air control channel L1 to deform the elastic membrane to form a normally closed channel. Positive pressure is input to the outer circle air control channel L2 to deform the elastic membrane to form a normally closed channel, which can be used for hybridization reaction in reaction pool R2 and secondary antibody binding reaction in pool R3, and prevent evaporation of reaction reagents.

[0108] Supply negative pressure to air nozzles A and B, input negative pressure to the inner ring air control channel L1 to deform the elastic membrane to form a normally open channel, and input negative pressure to the outer ring air control channel L2 to deform the elastic membrane to form a normally open channel, which can achieve precise transfer from R3 to the waste liquid pool R4.

[0109] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same and similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part description. The above description of the disclosed embodiments enables professionals and technicians in this field to implement or use the present invention. Various modifications to these embodiments will be apparent to professionals and technicians in this field, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will comply with the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A pump-valve mechanism for gas control of a microfluidic chip, characterized in that: include: A turntable housing (1), wherein a microfluidic chip (400) is fixed on the top surface of the turntable housing (1), and an air nozzle A (4001) and an air nozzle B (4002) are provided on the microfluidic chip (400) for supplying air to and exhausting air from the inner ring air control channel and the outer ring air control channel, respectively; An interface adapter (2), the interface adapter (2) being fixed inside the turntable housing (1), the interface adapter (2) having an A connector (201) and a B connector (202), the upper end of the A connector (201) being plugged into and connected to the A gas nozzle (4001), and the upper end of the B connector (202) being plugged into and connected to the B gas nozzle (4002); A pump-valve assembly (3) is fixed inside the turntable housing (1), and two air outlets of the pump-valve assembly (3) are respectively connected to the lower end of the A connector (201) and the lower end of the B connector (202).

2. A pump-valve mechanism for gas control of a microfluidic chip according to claim 1, characterized in that: The turntable housing (1) comprises: a turntable bottom shell (101), a turntable middle shell (102), and a turntable top shell (103); the turntable bottom shell (101) and the turntable top shell (103) are respectively fixed to the bottom end and the top end of the turntable middle shell (102); the microfluidic chip (400) is fixed to the bottom end surface of the turntable top shell (103); the interface adapter (2) is mounted on the turntable middle shell (102); and the pump valve assembly (3) is mounted on the top end surface of the turntable bottom shell (101).

3. The pump-valve mechanism for gas control of a microfluidic chip according to claim 2, characterized in that: The pump-valve assembly (3) comprises a positive pressure pump (301), a negative pressure pump (302), a first normally open valve (303), a second normally open valve (304), a first normally closed valve (305), a second normally closed valve (306), a third normally closed valve (307), a fourth normally closed valve (308), a first three-way pipe (309), a second three-way pipe (310), a third three-way pipe (311), and a fourth three-way pipe (312), all of which are mounted on the turntable bottom shell (101); The positive pressure pump (301) is connected to the third normally closed valve (307) via a first power line (313), the first normally open valve (303) is connected to the second normally closed valve (306) via a second power line (314), the negative pressure pump (302) is connected to the fourth normally closed valve (308) via a third power line (315), and the second normally open valve (304) is connected to the first normally closed valve (305) via a fourth power line (316); The positive pressure port of the positive pressure pump (301) is connected to the A port of the third normally closed valve (307) through a first air pipe (317), the B port of the third normally closed valve (307) is connected to the A port of the first three-way pipe (309) through a second air pipe (318), the B port of the first three-way pipe (309) is connected to the B port of the first normally closed valve (305) through a third air pipe (319), the A port of the first normally closed valve (305) is connected to the B port of the fourth three-way pipe (312) through a fourth air pipe (320), and the A port of the fourth three-way pipe (312) is in communication with the lower end of the B connector (202); The C port of the first three-way pipe (309) is connected to the B port of the first normally open valve (303) through the fifth air pipe (321), the A port of the first normally open valve (303) is connected to the C port of the second three-way pipe (310) through the sixth air pipe (322), the A port of the second three-way pipe (310) is communicated with the lower end of the A connector (201), the B port of the second three-way pipe (310) is connected to the A port of the second normally closed valve (306) through the seventh air pipe (323), and the B port of the second normally closed valve (306) is connected to the B port of the third three-way pipe (311) through the eighth air pipe (324); The negative pressure port of the negative pressure pump (302) is connected to the A port of the fourth normally closed valve (308) through a ninth air pipe (325), the B port of the fourth normally closed valve (308) is connected to the A port of the third three-way pipe (311) through a tenth air pipe (326), the C port of the third three-way pipe (311) is connected to the B port of the second normally open valve (304) through an eleventh air pipe (327), and the A port of the second normally open valve (304) is connected to the C port of the fourth three-way pipe (312) through a twelfth air pipe (328).

4. A centrifugal device, characterized in that: include: The pump-valve mechanism and the centrifugal drive unit (4) according to claim 3, wherein the driving spindle of the centrifugal drive unit (4) is fixedly connected to the pump-valve mechanism.

5. A centrifugal device according to claim 4, characterized in that: The centrifugal drive unit (4) comprises: A centrifugal motor protection bracket (401), wherein a centrifugal motor (402) is provided inside the centrifugal motor protection bracket (401); A main shaft support (403), wherein the bottom end of the main shaft support (403) is fixedly connected to the top of the centrifugal motor protection support (401), the main shaft support (403) is fixedly connected to the top of the housing of the centrifugal motor (402), a bearing (404) is fixed to the top of the main shaft support (403), a driving main shaft (4021) of the centrifugal motor (402) passes upward through the main shaft support (403) and is fixed to the inner ring of the bearing (404), and a connecting boss (40211) on the driving main shaft (4021) is fixedly connected to the turntable bottom shell (101); An electric slip ring (405) is provided, wherein the inner cylinder of the electric slip ring (405) is sleeved on the driving main shaft (4021) located in the main shaft support (403) section, the outer cylinder of the electric slip ring (405) is fixedly connected to the main shaft support (403) via an outer cylinder fixing frame (406), and the wires inside the inner cylinder of the electric slip ring (405) are electrically connected to the positive pressure pump (301), the negative pressure pump (302), the first normally open valve (303), the second normally open valve (304), the first normally closed valve (305), the second normally closed valve (306), the third normally closed valve (307), and the fourth normally closed valve (308).

6. A centrifugal device according to claim 5, characterized in that: The centrifugal drive unit (4) further comprises a centrifugal protective housing (407) which is arranged on the outer peripheral side of the turntable housing (1); the bottom shell of the centrifugal protective housing (407) is fixedly connected to the spindle support (403).

7. A centrifuge, characterized in that: include: counterweight base plate (5); The centrifugal device according to any one of claims 5 to 6, wherein the centrifugal motor protection bracket (401) is fixed on the counterweight base plate (5); A temperature control mechanism (6) is installed on the counterweight base plate (5) and is used to provide a heating or cooling analysis environment for the microfluidic chip (400).

8. A centrifuge according to claim 7, characterized in that: The counterweight base plate (5) is provided with a first centrifugal mounting hole, and the temperature control mechanism (6) comprises: A guide rail supporting base plate (601), the guide rail supporting base plate (601) being fixed to the top of the counterweight base plate (5), a second centrifugal mounting hole (6011) being provided on the guide rail supporting base plate (601) at a position corresponding to the first centrifugal mounting hole, and the centrifugal motor protection bracket (401) being fixed to the edge of the second centrifugal mounting hole (6011) via the mounting ear plate (4011) thereon; Guide rail supporting side plates (602), the guide rail supporting side plates (602) being two fixed at intervals on both sides of the guide rail supporting bottom plate (601), the centrifugal device being located between the two guide rail supporting side plates (602), and linear guide rails (603) being installed on opposite side walls of the two guide rail supporting side plates (602); A temperature-controlled cover translation mechanism (604), wherein the translation connecting plates (60412) on both side walls of the temperature-controlled cover translation mechanism (604) are respectively fixedly connected to the sliders on the two linear slide rails (603); A translation drive mechanism (605) is fixed on one of the guide rail support side plates (602), and a translation portion of the translation drive mechanism (605) is fixed to one of the translation connection plates (60412), and is used to drive the temperature control cover translation mechanism (604) to translate to above the microfluidic chip (400), so as to provide a heating or cooling analysis environment for the microfluidic chip (400).

9. A centrifuge according to claim 8, characterized in that: The temperature control cover translation mechanism (604) comprises: The upper shell fixed mold assembly (6041) includes: An upper shell (60411), the translation connecting plates (60412) are fixed on both side walls of the upper shell (60411), and a plurality of guide rods (60413) are evenly distributed and fixed on the inner top wall of the upper shell (60411); A screw lifting motor (60414), wherein the housing of the screw lifting motor (60414) is fixed to the top of the upper shell (60411), and the screw (604141) of the screw lifting motor (60414) passes through the upper shell (60411) and extends downward; The lower shell movable module (6042) includes: A connecting disk (60421) is provided with a screw rod insertion hole (604211) in the middle of the connecting disk (60421), a screw rod nut (60422) is fixed on the screw rod insertion hole (604211), the screw rod nut (60422) is threadedly connected to the screw rod (604141), and a plurality of guide cylinders (60423) are evenly distributed and fixed on the top of the connecting disk (60421), and the plurality of guide rods (60413) are plugged into the plurality of guide cylinders (60423); A cooling plate (60424), wherein the cooling plate (60424) is fixed at intervals on the bottom end surface of the connecting plate (60421); A cooling water pipe (60425), the cooling water pipe (60425) is arranged between the connecting plate (60421) and the cooling plate (60424), and the cooling water pipe (60425) is fixed on the top of the cooling plate (60424), and the two ends of the cooling water pipe (60425) are respectively connected to the water inlet and outlet of the external cooling host (7); A heating block (60426) is fixed on the bottom surface of the connecting plate (60421), a PI heating film (60427) is fixed on the top surface of the heating block (60426), and a temperature sensor (60428) for monitoring its temperature is installed on the PI heating film (60427).

10. A centrifuge according to claim 9, characterized in that: One of the guide rail support side plates (602) is provided with an elongated hole (6021), a lower clamping block (8) is fixed on one of the translation connecting plates (60412), an upper clamping block (9) is fixed on the lower clamping block (8), and the lower clamping block (8) is arranged through the elongated hole (6021), and the translation driving mechanism (605) includes: a translation motor (6051), the translation motor (6051) being fixed on a side wall of one of the guide rail supporting side plates (602); A driving pulley (6052), wherein the driving wheel shaft of the driving pulley (6052) is rotatably mounted on the other side wall of one of the guide rail supporting side plates (602), and the driving wheel shaft is fixedly connected to the driving shaft of the translation motor (6051); A driven pulley (6053), wherein the driven pulley shaft of the driven pulley (6053) is rotatably mounted on the other side wall of one of the guide rail supporting side plates (602); A translation synchronous belt (6054), wherein the translation synchronous belt (6054) is sleeved on the driving pulley (6052) and the driven pulley (6053), and an upper belt (60541) of the translation synchronous belt (6054) is clamped between the lower clamping block (8) and the upper clamping block (9) by bolts; A drag chain (6055), wherein the fixed end of the drag chain (6055) is fixed to the top of one of the guide rail supporting side plates (602), and the free end of the drag chain (6055) is fixedly connected to one of the translation connecting plates (60412) via a connecting piece (6056).

Images