A self-checking kit and a method for preparing the same

Abstract

The application provides a self-checking kit and a preparation method thereof. The self-checking kit comprises a main body, a sample driving film, a moving unit, a detection line T and a quality control line C. A recess is arranged on the lower surface of the main body. The sample driving film covers the lower surface of the main body. The recess and the sample driving film jointly constitute a closed cavity for sample flow. The surface of the sample driving film is laser etched to form a super-hydrophobic micro-nano double structure. The sample driving film is loaded with a wave potential. The upper surface of the main body is provided with a sample adding hole which is in communication with the cavity on one side and is provided with the moving unit on the other side. The moving unit is rotationally connected with the main body. The side of the moving unit close to the cavity is provided with the quality control line C. The cavity is provided with the detection line T opposite to the quality control line C. The application has the technical effect of improving the detection efficiency, reducing the sample consumption and saving the cost.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of liquid detection, and particularly relates to a self-test kit and a preparation method thereof. BACKGROUND

[0002] The self-test kit is a box for containing chemical reagents for detecting chemical components, drug residues, virus types, etc. For specific viruses, it has corresponding antigen-antibody reactions and can be used for screening of specific groups of people, which is conducive to improving the ability of early detection. The self-test kit is convenient to use, and an individual can complete the detection in 8 steps at home. It can be used for sampling and detection of oropharyngeal swabs and nasopharyngeal swabs, and the result can be obtained in 15 minutes at the fastest. The emergence of the self-test kit greatly reduces the secondary infection of medical staff and improves the efficiency and level of treatment and prevention.

[0003] The existing self-test kit uses fluorescence immunochromatography or colloidal gold method to realize pathological self-detection. The liquid drop driving method used in the fluorescence immunochromatography and colloidal gold method is chromatography, that is, a water-absorbing filter paper is used as a sample pad, and the capillary action of the liquid drop is used to drive the sample flow. In the process of driving the sample flow, the sample is combined with the fluorescent antibody or colloidal gold, and then detected and developed color on the detection line and the quality control line, respectively.

[0004] However, the existing self-test kit using the liquid capillary phenomenon as the driving force has the problems of weak driving force, slow liquid flow speed, long detection time, and greatly reduced detection efficiency. At the same time, the capillary phenomenon of the liquid sample on the water-absorbing filter membrane is very short. In order to effectively detect and react, a large amount of liquid sample needs to be added to drive it to the detection line and the quality control line, which is a great waste and loss for very rare samples to be detected. SUMMARY

[0005] The present application aims to at least solve one of the technical problems existing in the prior art, and provide a new technical solution of a self-test kit and a preparation method thereof.

[0006] According to a first aspect of the present application, a self-test kit is provided, which comprises a main body, a sample driving film, a moving unit, a detection line T and a quality control line C.

[0007] The lower surface of the main body is provided with a groove, and the sample driving film covers the lower surface of the main body. The groove and the sample driving film together constitute a closed chamber for sample flow. The surface of the sample driving film is laser etched to form a super-hydrophobic micro-nano double structure, so that the liquid drop of the sample to be detected forms a spherical three-dimensional structure on the surface of the sample driving film, and the sample driving film is loaded with a wave-shaped electric potential.

[0008] The upper surface of the main body is provided with a sample adding hole communicated with the cavity, and the other side is provided with a moving unit, which is rotationally connected with the main body, and the cavity can be closed or opened by rotating the moving unit.

[0009] The side of the moving unit close to the cavity is provided with the quality control line C, and the position opposite to the quality control line C of the cavity is provided with the detection line T, and a preset gap is formed between the quality control line C and the detection line T.

[0010] When the self-checking kit is detected, the sample adding hole is used to add the sample to be detected, and the droplet of the sample to be detected in the spherical three-dimensional structure flows along the cavity to the preset gap under the driving of the wavy electric potential electrostatic force on the surface of the sample driving film, and the color reaction occurs with the detection line T and the quality control line C at the same time; the color change of the detection line T and the quality control line C can be observed by rotating the moving unit.

[0011] Optionally, the contact angle of the droplet of the sample to be detected with the surface of the sample driving film is 150°-180°.

[0012] Optionally, the material of the sample driving film is at least one of polydimethylsiloxane, polytetrafluoroethylene, polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene.

[0013] Optionally, the surface of the sample driving film is laser etched at a power of 700W, and the surface of the sample driving film is laser etched in an array block with a side length of 10-100 microns.

[0014] Optionally, the depth of laser etching on the surface of the sample driving film is 2-20 microns.

[0015] Optionally, when the surface of the sample driving film is laser etched, nano-like particles are spontaneously assembled on the array block to form a super-hydrophobic micro-nano double structure.

[0016] Optionally, the moving unit is a long strip, and one end of the moving unit is rotationally connected with the main body.

[0017] When the moving unit is rotated to open the cavity, the quality control line C is located on the upper surface of the moving unit.

[0018] According to the second aspect of the application, a preparation method of a self-checking kit is provided for manufacturing the self-checking kit as described in the first aspect, comprising the following steps:

[0019] Step S100, preparing a sample driving film: first, laser etching is performed on the surface of the sample driving film to form a super-hydrophobic micro-nano double structure; then, the sample driving film is placed between a single-needle electrode and a grounding electrode, the sample driving film is charged by the single-needle electrode loaded with positive high voltage to polarize the sample driving film, then, the relative position of the single-needle electrode and the sample driving film is changed, the sample driving film is charged by the single-needle electrode loaded with negative high voltage to form a wavy electric potential on the sample driving film;

[0020] Step S200, installing the sample driving film on the lower surface of the main body;

[0021] Step S300, first, rotating the moving unit to open the cavity, sticking a quality control line C on the side of the moving unit close to the cavity, and sticking a detection line T on the inner side of the main body opposite to the quality control line C; then, rotating the moving unit to close the cavity.

[0022] Optionally, the number of first point positions of the single-needle electrode loaded with positive high voltage to charge the sample driving film is 2-4; the number of second point positions of the single-needle electrode loaded with negative high voltage to charge the sample driving film is 2-4, and the first point positions and the second point positions are alternately distributed.

[0023] Optionally, when charging the sample driving film, the distance between the sample driving film and the single-needle electrode is 5 mm; the charging time of a certain position of the sample driving film is 5 minutes, and the environmental temperature of the charging is 90 DEG.

[0024] One technical effect of the present application is that:

[0025] In the embodiment of the present application, the surface of the sample driving film of the self-test kit is formed with a super-hydrophobic micro-nano double structure, so that the droplets of the sample to be detected form a spherical three-dimensional structure on the surface of the sample driving film, so that the sample driving film has low adhesion and low rolling resistance to the liquid sample; and the sample driving film is loaded with a wavy electric potential. Due to the low adhesion of the sample driving film with the super-hydrophobic micro-nano double structure to the liquid sample, the driving resistance of the sample driving film to the sample droplets is greatly reduced, further, the wavy electric potential on the sample driving film has strong electrostatic force, which can significantly accelerate the driving rate of the liquid sample, thereby improving the detection efficiency and reducing the detection time.

[0026] In addition, due to the low adhesion and low rolling resistance of the sample driving film to the liquid sample, the adsorption of the sample driving film to the liquid sample is greatly reduced, not only effectively reducing the amount of sample and saving cost, but also preventing the sample from polluting the self-test kit, so that the self-test kit can be reused after replacing the test paper, which is convenient to operate and helps to reduce the cost. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 Structure diagram of a self-checking kit according to an embodiment of the present application;

[0028] Figure 2 Structure diagram of a self-checking kit according to an embodiment of the present application;

[0029] Figure 3 Structure diagram of a self-checking kit according to an embodiment of the present application;

[0030] Figure 4 SEM image of the micro-nano double structure of the super-hydrophobic sample driving film of a self-checking kit according to an embodiment of the present application;

[0031] Figure 5 Diagram of the undulating electric potential of the sample driving film of a self-checking kit according to an embodiment of the present application;

[0032] Figure 6 Diagram of the undulating electric potential of the sample driving film of a self-checking kit according to an embodiment of the present application.

[0033] In the figure: 1, main body; 101, sample hole; 102, chamber; 103, preset gap; 2, sample driving film; 3, moving unit; 4, detection line T; 5, quality control line C; 6, spherical three-dimensional structure; 7, undulating electric potential; 8, array block; 9, single needle electrode. DETAILED DESCRIPTION

[0034] Various exemplary embodiments of the present application will now be described in detail with reference to the accompanying drawings. It should be noted that the relative arrangement of the components and steps set forth in these embodiments, numerical expressions, and numerical values are not limiting to the scope of the present application unless specifically stated otherwise.

[0035] The embodiments of the present application will be described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar notations represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by reference to the accompanying drawings are exemplary only, for the purpose of explanation, and are not to be understood as limiting to the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative labor fall within the scope of the present application.

[0036] The terms "first", "second" in the description and claims of the present application can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specified. In addition, "and / or" in the specification and claims indicates at least one of the connected objects, and the character " / ", generally indicates that the front and rear associated objects are in an "or" relationship.

[0037] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0038] In the description of the present application, it should be noted that unless otherwise specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0039] According to the first aspect of the present application, referring to Figures 1 to 6 , a self-checking kit is provided, which comprises a main body 1, a sample driving film 2, a moving unit 3, a detection line T4 and a quality control line C5; wherein the detection line T4 and the quality control line C5 are both strip-shaped test papers, that is, the liquid sample passes through the quality control line C5 and shows that the test paper is valid, and passes through the detection line T4 and shows that the collected liquid sample contains antigens, that is, viral proteins;

[0040] The lower surface of the main body 1 is provided with a groove, and the sample driving film 2 covers the lower surface of the main body 1, and the groove and the sample driving film 2 together constitute a closed chamber 102 for sample flow; the surface of the sample driving film 2 is laser etched to form a super-hydrophobic micro-nano double structure, so that the droplet of the sample to be detected forms a spherical three-dimensional structure 6 on the surface of the sample driving film 2, and the sample driving film 2 is loaded with a wave potential 7;

[0041] The upper surface of the main body 1 is provided with a sample adding hole 101 communicating with the chamber 102, and the other side is provided with a moving unit 3, which is rotationally connected with the main body 1, and the chamber 102 can be closed or opened by rotating the moving unit 3;

[0042] The side of the moving unit 3 close to the chamber 102 is provided with the quality control line C5, and the position opposite to the quality control line C5 of the chamber 102 is provided with the detection line T4, and a preset gap 103 is formed between the quality control line C5 and the detection line T4;

[0043] When detecting, the sample adding hole 101 is added with the sample to be detected, and the droplet of the sample to be detected in the spherical three-dimensional structure 6 on the surface of the sample driving film 2 flows into the preset gap 103 along the chamber 102 under the driving of the electrostatic force of the undulating electric potential 7, and the color reaction occurs with the detection line T4 and the quality control line C5 at the same time, so as to complete the double detection of the sample; the color change of the detection line T4 and the quality control line C5 can be observed by rotating the moving unit 3.

[0044] In the embodiment of the present application, the surface of the sample driving film 2 of the self-checking kit is formed with a super-hydrophobic micro-nano double structure, so that the droplet of the sample to be detected forms a spherical three-dimensional structure 6 on the surface of the sample driving film 2, so that the sample driving film 2 has low adhesion and low rolling resistance to the liquid sample; and the sample driving film 2 is loaded with an undulating electric potential 7. Due to the low adhesion of the super-hydrophobic micro-nano double structure of the sample driving film 2 to the liquid sample, the driving resistance of the sample driving film 2 to the sample droplet is greatly reduced, and further, the undulating electric potential 7 on the sample driving film 2 has strong electrostatic force, which can significantly accelerate the driving rate of the liquid sample, thereby improving the detection efficiency and reducing the detection time.

[0045] In addition, due to the low adhesion and low rolling resistance of the sample driving film 2 to the liquid sample, the adsorption of the sample driving film 2 to the liquid sample is greatly reduced, not only effectively reducing the amount of sample and saving cost, but also preventing the sample from polluting the self-checking kit, so that the self-checking kit can be reused after replacing the test paper, which is convenient to operate and helps to reduce the cost.

[0046] Optionally, the contact angle of the droplet of the sample to be detected with the surface of the sample driving film 2 is 150°-180°.

[0047] In the above embodiment, the low adhesion and low rolling resistance of the sample driving film 2 to the liquid sample are improved, which greatly reduces the driving resistance of the sample driving film 2 to the sample droplet, improves the detection efficiency, and reduces the adsorption of the sample driving film 2 to the liquid sample, thereby effectively reducing the sample amount and saving costs.

[0048] Optionally, the sample driving film 2 is made of at least one of polydimethylsiloxane, polytetrafluoroethylene, polyvinylidene fluoride, and polyvinylidene fluoride-trifluoroethylene.

[0049] In the above embodiment, the low adhesion and low rolling resistance of the sample driving film 2 to the liquid sample are improved, which greatly reduces the driving resistance of the sample driving film 2 to the sample droplet, improves the detection efficiency, and reduces the adsorption of the sample driving film 2 to the liquid sample, thereby effectively reducing the sample amount and saving costs.

[0050] Optionally, the surface of the sample driving film 2 is laser etched at a power of 700W, and the surface of the sample driving film 2 is laser etched in an array block 8 with a side length of 10-100 microns.

[0051] In the above embodiment, the low adhesion and low rolling resistance of the sample driving film 2 to the liquid sample are improved, which greatly reduces the driving resistance of the sample driving film 2 to the sample droplet, improves the detection efficiency, and reduces the adsorption of the sample driving film 2 to the liquid sample, thereby effectively reducing the sample amount and saving costs.

[0052] Optionally, the surface of the sample driving film 2 is laser etched at a depth of 2-20 microns. This makes the laser etching depth more appropriate, and helps to form a super-hydrophobic micro-nano double structure on the surface of the sample driving film 2 during laser etching.

[0053] Optionally, during laser etching, nano-sized particles (100nm in size) spontaneously assemble on the array block 8 to form a super-hydrophobic micro-nano double structure. This makes the way of forming a super-hydrophobic micro-nano double structure on the surface of the sample driving film 2 relatively simple, and helps to achieve the low adhesion and low rolling resistance of the sample driving film 2 to the liquid sample.

[0054] Optionally, the moving unit 3 is in a long strip shape, and one end of the moving unit 3 is rotatably connected to the main body 1.

[0055] When the moving unit 3 is rotated to open the chamber 102, the quality control line C5 is located on the upper surface of the moving unit 3.

[0056] In the above embodiment, the chamber 102 can be quickly opened or closed by rotating the moving unit 3, and when the chamber 102 is opened, not only the viewing of the control line C5 and the detection line T4 is achieved, the operation is simple, but also the replacement of the control line C5 and the detection line T4 is facilitated, the detection efficiency is improved, and the detection cost is saved.

[0057] In the embodiment of the present application, the use steps of the self-checking kit are as follows:

[0058] Firstly, 5 microliters of sodium hydroxide droplets (NaOH) are added to the sample hole 101, and the droplets form a spherical three-dimensional structure 6 on the sample driving film 2.

[0059] Then, under the action of the undulating electric potential 7, the droplets are spontaneously transported at high speed to the control line C5 and the detection line T4, and the time is less than 1s.

[0060] Then, by using the spherical three-dimensional structure 6 of the sample on the sample driving film 2, the droplets respectively perform color development reactions with the detection line FeCl3 and the control line pH, and the time is less than 10s.

[0061] Finally, after the sample detection is completed, the moving unit 3 is opened, and the color change of the detection line T4 and the control line C5 can be viewed.

[0062] According to the second aspect of the present application, a preparation method of a self-checking kit is provided for manufacturing the self-checking kit as described in the first aspect, comprising the following steps:

[0063] Step S100, preparing the sample driving film 2: first, the surface of the sample driving film 2 is laser etched to form a super-hydrophobic micro-nano double structure, see Figure 4 ; then, the sample driving film 2 is placed between the single-needle electrode 9 and the ground electrode, the sample driving film 2 is charged by the single-needle electrode 9 with positive high voltage to polarize the sample driving film 2, then the relative position of the single-needle electrode 9 and the sample driving film 2 is changed, the sample driving film 2 is charged by the single-needle electrode 9 with negative high voltage to form the undulating electric potential 7 on the sample driving film 2, see Figure 5 and Figure 6 .

[0064] It should be noted that, Figure 4 a) in the above-mentioned drawings, a) is a scanning electron microscope image of the sample driving film 2; b), c), and d) are further enlarged views of the a) image; and the inserted image in the d) image is the shape of the droplet of the sample on the surface of the sample driving film 2,

[0065] Step S200, installing the sample driving film 2 on the lower surface of the main body 1.

[0066] Step S300, first, rotate the moving unit 3 to open the chamber 102, and stick the quality control line C5 on the side of the moving unit 3 close to the chamber 102, and stick the detection line T4 on the inner side of the main body 1 opposite to the quality control line C5; then, rotate the moving unit 3 to close the chamber 102.

[0067] In the above embodiment, the preparation method of the self-test kit is very simple. Moreover, the prepared self-test kit can not only significantly accelerate the driving speed of the liquid sample, thereby improving the detection efficiency and reducing the detection time. Moreover, the adsorption of the sample driving film 2 to the liquid sample is greatly reduced, not only effectively reducing the sample usage and saving costs, but also preventing the sample from polluting the self-test kit, so that the self-test kit can be reused after the test paper is simply replaced, which is convenient to operate and helps to reduce costs.

[0068] In a specific embodiment, when the undulating electric potential 7 is loaded on the sample driving film 2, first, a polytetrafluoroethylene (PTFE) film is placed between the single needle electrode 9 and the ground electrode, and the polarization of the polytetrafluoroethylene film is performed by using corona high voltage (0-20KV) charging. For example, the polytetrafluoroethylene film is charged by the single needle electrode 9 with a 6V power source for 5min at 90°C, wherein the charging voltage of the single needle electrode 9 is amplified by 1000 times by a triode, and the distance between the polytetrafluoroethylene film and the single needle electrode 9 is maintained at about 5mm. Therefore, due to the single discharge of the single needle electrode 9, the electric potential naturally reaches a peak at the point center of the sample driving film 2 corresponding to the single needle electrode 9 and decays to the surrounding environment.

[0069] Secondly, in order to obtain the undulating electric potential 7, the relative position of the single needle electrode 9 and the polytetrafluoroethylene film is moved, and the polytetrafluoroethylene film is charged by using negative high voltage (-20-0KV). For example, the polytetrafluoroethylene film is charged by the single needle electrode 9 with a -6V power source for 5min at 90°C, wherein the charging voltage of the single needle electrode 9 is amplified by 1000 times by a triode, and the distance between the polytetrafluoroethylene film and the single needle electrode 9 is maintained at about 5mm. Therefore, due to the single discharge of the single needle electrode 9, the electric potential naturally reaches a peak at the point center of the sample driving film 2 corresponding to the single needle electrode 9 and decays to the surrounding environment.

[0070] Alternatively, the polytetrafluoroethylene film or the polyvinylidene fluoride film is laser etched by the array block 8 with a side length of 50 microns at a power of 700W, and the etching depth is 10 microns.

[0071] In other embodiments, the polytetrafluoroethylene film is charged by the single needle electrode 9 with a 4V power source for 5min at 90°C. Then, for example, the polytetrafluoroethylene film is charged by the single needle electrode 9 with a -4V power source for 5min at 90°C.

[0072] Optionally, the material of the sample driving film 2 is polyvinylidene fluoride trifluoroethylene (PVDF-TrFE), and the charging voltage is increased to 8V.

[0073] Optionally, the number of the first point positions of the sample driving film 2 charged by the single needle electrode 9 with positive high voltage is 2-4; the number of the second point positions of the sample driving film 2 charged by the single needle electrode 9 with negative high voltage is 2-4, and the first point positions and the second point positions are alternately distributed.

[0074] In the above embodiment, the wave-shaped electric potential 7 is formed on the sample driving film 2, so as to realize the electrostatic force on the liquid droplet of the sample to be detected, thereby providing the driving rate of the liquid sample and reducing the detection time.

[0075] It should be noted that, referring to Figure 6 , the wave peak is the first point position of the sample driving film 2, and the wave valley is the second point position of the sample driving film 2.

[0076] Optionally, when the sample driving film 2 is charged, the distance between the sample driving film 2 and the single needle electrode 9 is 5mm; the charging time of a certain position of the sample driving film 2 is 5 minutes, and the environmental temperature of the charging is 90°.

[0077] In the above embodiment, the effect of forming the wave-shaped electric potential 7 on the sample driving film 2 is guaranteed, so as to ensure that the driving rate of the liquid sample is improved by the electrostatic force on the liquid droplet of the sample to be detected, thereby significantly improving the detection efficiency.

[0078] It can be understood that the above embodiments are only exemplary embodiments for illustrating the principles of the present application, and the present application is not limited thereto. Various modifications and improvements can be made by those skilled in the art without departing from the spirit and essence of the present application, and these modifications and improvements are also regarded as the protection scope of the present application.

Claims

1. A self-test kit, characterized in that, The self-checking kit comprises a main body, a sample driving film, a moving unit, a detection line T and a quality control line C. A groove is arranged on the lower surface of the main body, and the sample driving film covers the lower surface of the main body, the groove and the sample driving film jointly constitute a closed chamber for sample flow; the surface of the sample driving film is laser etched to form a super-hydrophobic micro-nano double structure, so that the droplet of the sample to be detected forms a spherical three-dimensional structure on the surface of the sample driving film, and the sample driving film is loaded with a wave-shaped potential; The upper surface of the main body is provided with a sample hole communicating with the chamber on one side, and is provided with a moving unit on the other side, the moving unit is rotatably connected with the main body, and the chamber can be closed or opened by rotating the moving unit; The side of the moving unit close to the chamber is provided with the quality control line C, the chamber is provided with the detection line T opposite to the quality control line C, and a preset gap is formed between the quality control line C and the detection line T; When detecting, the sample to be detected is added through the sample hole, the droplet of the sample to be detected in the spherical three-dimensional structure flows along the chamber to the preset gap under the driving of the electrostatic force of the wave-shaped potential on the surface of the sample driving film, and the color development reaction occurs with the detection line T and the quality control line C at the same time; the color change of the detection line T and the quality control line C can be observed by rotating the moving unit.

2. The self-test kit of claim 1, wherein, The contact angle of the droplet of the sample to be detected with the surface of the sample driving film is 150°-180°.

3. The self-test kit of claim 1, wherein, The material of the sample driving film is at least one of polydimethylsiloxane, polytetrafluoroethylene, polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene.

4. The self-test kit of claim 1, wherein, The power of laser etching on the surface of the sample driving film is 700W, and the surface of the sample driving film is laser etched in an array block with a side length of 10-100 microns.

5. The self-test kit of claim 4, wherein, The depth of laser etching on the surface of the sample driving film is 2-20 microns.

6. The self-test kit of claim 4, wherein, When laser etching the surface of the sample driving film, nano particles spontaneously assemble on the array block to form a super-hydrophobic micro-nano double structure.

7. The self-testing kit of claim 1, wherein The moving unit is a long strip, and one end of the moving unit is rotatably connected with the main body. When the chamber is opened by rotating the moving unit, the quality control line C is located on the upper surface of the moving unit.

8. A method of manufacturing a self-test kit, characterized by, A method for manufacturing the self-checking kit as claimed in any one of claims 1 to 7 comprises the following steps: In step S100, the surface of the sample driving film is laser etched to form a super-hydrophobic micro-nano double structure; then, the sample driving film is placed between a single needle electrode and a grounding electrode, the sample driving film is charged by the single needle electrode with positive high voltage to polarize the sample driving film, then the relative position of the single needle electrode and the sample driving film is changed, and the sample driving film is charged by the single needle electrode with negative high voltage to form a wave-shaped potential on the sample driving film; In step S200, the sample driving film is installed on the lower surface of the main body. Step S300, first, rotate the moving unit to open the chamber, in the moving unit close to the chamber side pasted quality control line C, and in the chamber with the quality control line C opposite position pasted detection line T; then, rotate the moving unit to close the chamber.

9. The method of claim 8, wherein the self-test kit is prepared by, The number of the first point positions of the single needle electrode pair loaded with positive high voltage for charging the sample driving film is 2-4; the number of the second point positions of the single needle electrode pair loaded with negative high voltage for charging the sample driving film is 2-4, and the first point positions and the second point positions are alternately distributed.

10. The method of claim 9, wherein the self-test kit is prepared by, When charging the sample driving film, the distance between the sample driving film and the single needle electrode is 5mm; the charging time of a certain position of the sample driving film is 5 minutes, and the environmental temperature of the charging is 90°.

Images