A concentrated acidic phenolic disinfectant and its preparation method

Abstract

The present invention discloses a concentrated acidic phenolic disinfectant and a preparation method thereof, belonging to the technical field of disinfectants, and characterized by comprising a compound phenolic compound, a dispersant, a surfactant, a compound organic acid and the balance of water; the compound phenolic compound is a mixture of phenol and triclosan; the dispersant is a mixture of ammonium cetyl sulfate or sorbitan fatty acid ester; the compound organic acid is a mixture of L-malic acid and citric acid; the dispersant is ethanol; the beneficial effects of the present invention are: ammonium cetyl sulfate and sorbitan fatty acid ester as a compound dispersant can promote the solubility of the compound phenolic compound of phenol and triclosan in the ethanol system; ammonium cetyl sulfate and sorbitan fatty acid ester as a compound dispersant can promote the bactericidal effect of the compound phenolic compound of phenol and triclosan.

Description

Technical Field:

[0001] The present invention belongs to the technical field of disinfectants, and more particularly relates to a concentrated acidic phenolic disinfectant and a preparation method thereof. Background Art:

[0002] Acidic phenolic disinfectant is a disinfectant that can be used for general object surfaces and fabrics. Its main components include phenolic compounds. When in use, the disinfectant can be diluted with standard hard water in a certain proportion and has activity against microorganisms such as bacteria including Mycobacterium, fungi including Aspergillus niger, and viruses.

[0003] Phenol is a compound formed by substituting a hydrogen atom on the benzene ring with a hydroxyl group, and can be classified into monohydric phenol, dihydric phenol, trihydric phenol, etc. according to the number of hydroxyl groups contained on the benzene ring. Phenolic compounds can cause bacteria to die by adsorbing to the bacterial cell wall, penetrating the cell wall, reducing the surface tension of the solution, increasing the permeability of the cell membrane, dissolving the lipid layer of the cytoplasmic membrane, causing cytoplasmic damage, and spilling of cell contents. There are also studies showing that certain phenolic substances can undergo irreversible chemical reactions with the free amino groups in bacterial cells, resulting in highly denatured proteins and causing the death of bacterial cells.

[0004] The bactericidal ability of phenolic substances is closely related to their chemical structure. Generally, it gradually weakens with the increase of hydroxyl groups on the benzene ring, while the disinfection ability is enhanced when methyl or halogen is introduced into the benzene ring. Currently, commonly used phenolic compounds mainly include: phenol, cresol, xylenol, dimethylphenol, o-phenylphenol, p-chloro-m-xylenol, polyethoxynonylphenol, nonylphenol polyethoxyl ether, triclosan, etc. It can be used in hospitals and medical-related fields, such as clinics, medical centers, pharmaceutical factories, and sterile rooms; it can also be used for daily life, such as for disinfecting areas like bathrooms and laundries or for clothing disinfection.

[0005] So far, phenolic disinfectants have been used for disinfecting hands, skin, clothing, and various object surfaces, but most of them are neutral products. Long-term use of disinfectants with a single property is likely to cause microorganisms to develop tolerance to the disinfectant. At the same time, phenolic substances will react with oxygen in the air and be gradually oxidized into quinone substances, resulting in color change and weakened disinfection effect. Summary of the Invention:

[0006] To solve the above problems and overcome the deficiencies of the prior art, the present invention provides a concentrated acidic phenolic disinfectant and a preparation method thereof, which can effectively solve the problems that the existing phenolic disinfectants are neutral products, and long-term use of disinfectants with a single property is likely to cause microorganisms to develop tolerance to the disinfectant. At the same time, phenolic substances will react with oxygen in the air and be gradually oxidized into quinone substances, resulting in color change and weakened disinfection effect.

[0007] The specific technical solution of the present invention to solve the above technical problems is as follows: A concentrated acidic phenolic disinfectant includes a compound phenolic compound, a dispersant, a surfactant, a compound organic acid, and the balance of water;

[0008] The compound phenolic compound is a mixture of phenol and triclosan;

[0009] The dispersant is a mixture of ammonium lauryl sulfate or sorbitan fatty acid ester;

[0010] The compound organic acid is a mixture of L-malic acid and citric acid;

[0011] The dispersant is ethanol;

[0012] The components by weight percentage are: 0.1%-40% of the compound phenolic compound, 0.1-40% of the dispersant, 0.1%-25% of the compound organic acid, and the balance is water.

[0013] The preparation method of the concentrated acidic phenolic disinfectant includes:

[0014] S1. Weigh the compound phenolic compound and the dispersant according to the amount and place them in a container, and stir and dissolve until completely dissolved;

[0015] S2. Accurately weigh the surfactant and add it to the product in step S1, and stir well;

[0016] S3. Accurately weigh the compound organic acid and add it to the product in step S2, and stir well;

[0017] S4. Accurately weigh the remaining mass of deionized water and add it to the product in S3, and stir well.

[0018] The beneficial effects of the present invention are:

[0019] (1) In terms of appearance and properties:

[0020] Ammonium lauryl sulfate and sorbitan fatty acid ester as a compound dispersant can promote the solubility of the compound phenolic compound of phenol and triclosan in the ethanol system;

[0021] In terms of bactericidal effect:

[0022] Ammonium cetyl sulfate and sorbitan fatty acid esters as compound dispersants can promote the bactericidal effect of compound phenolic compounds of phenol and triclosan;

[0023] (2) The compound organic acid composed of L-malic acid and citric acid itself has a synergistic effect on promoting the solubility of "compound phenolic compounds of ammonium cetyl sulfate and sorbitan fatty acid esters" in the ethanol system.

[0024] (3) In the present invention, one or more phenolic compounds are dissolved in deionized water, and a dispersant and an acidic substance are added to enhance the bactericidal effect and maintain the stability of the phenolic compounds. Double-layer packaging and irradiation are carried out to ensure strict sterility of the product. The sample is a concentrated solution, which is convenient for transportation and storage. It can be diluted with hard water at a ratio of 1:128 and used, and the disinfection effect can be qualified within 20 days when stored sealed after dilution. BRIEF DESCRIPTION OF THE DRAWINGS:

[0025] Attached Figure 1 is the experimental result diagram of the embodiment of the present invention;

[0026] Attached Figure 2 is the experimental result diagram of Comparative Examples 1-3;

[0027] Attached Figure 3 is the experimental result diagram of Comparative Examples 4-6;

[0028] Attached Figure 4 is the experimental result diagram of Comparative Examples 7-9;

[0029] Attached Figure 5 is the experimental result diagram of Comparative Examples 10-12; DETAILED DESCRIPTION OF THE INVENTION:

[0030] In the description of the present invention, specific details are only for fully understanding the embodiments of the present invention. However, those skilled in the art should know that the implementation of the present invention is not limited to these details. In addition, well-known structures and functions are not described or shown in detail to avoid obscuring the key points of the embodiments of the present invention. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] DETAILED DESCRIPTION OF THE INVENTION:

[0032] For better understanding of the present invention, specific embodiments are used for illustration. It should be emphasized that the effects of these embodiments are not substantially different from those of various embodiments within the protection scope of the present invention, including the respective reagents and the content ratios of the reagents, and all can achieve the effects described in the present invention and solve the above problems. Other combinations are not described in detail here;

[0033] To more intuitively demonstrate the process advantages of the present invention, a comparison is made between the concentrated acidic phenolic disinfectant and its preparation method of the present invention and an equivalent replacement method using the same process.

[0034] Example 1:

[0035] A concentrated acidic phenolic disinfectant comprises a compound phenolic compound, a dispersant, a surfactant, a compound organic acid, and the balance water;

[0036] The compound phenolic compound is a mixture of phenol and triclosan;

[0037] The dispersant is a mixture of ammonium lauryl sulfate or sorbitan fatty acid ester;

[0038] The compound organic acid is a mixture of L - malic acid and citric acid;

[0039] The components by weight percentage are: 0.1% of the compound phenolic compound, 0.1% of the dispersant, 0.1% of the compound organic acid, and the balance is water.

[0040] The preparation method of the concentrated acidic phenolic disinfectant comprises:

[0041] S1. Weigh the compound phenolic compound and the dispersant by quantity and place them in a container, stir and dissolve until completely dissolved;

[0042] S2. Accurately weigh the surfactant, add it to the product in step S1, and stir well;

[0043] S3. Accurately weigh the compound organic acid, add it to the product in step S2, and stir well;

[0044] S4. Accurately weigh the remaining mass of deionized water, add it to the product in S3, and stir well.

[0045] Example 2:

[0046] Same as Example 1, except that the components by weight percentage are: 40% of the compound phenolic compound, 40% of the dispersant, 25% of the compound organic acid, and the balance is water.

[0047] Example 3:

[0048] Same as Example 1, the difference is that: the components by weight percentage are: 20% of the compound phenolic compound, 20% of the dispersant, 15% of the compound organic acid, and the balance is water.

[0049] The specific embodiments of the present invention are:

[0050] The preparation method of the concentrated acidic phenolic disinfectant comprises:

[0051] S1. Weigh accurately 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container. Stir and dissolve until completely dissolved.

[0052] S2. Weigh accurately 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, and add them to the product in step S1. Stir well.

[0053] S3. Weigh accurately 10 g of L - malic acid and 10 g of citric acid, and add them to the product in step S2. Stir well.

[0054] S4. Weigh accurately the remaining mass of deionized water and add it to the product in S3. Stir well.

[0055] Comparative Example 1:

[0056] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, the types of phenolic compounds added are different, and no surfactant is added, especially no composite organic acid is added; specifically:

[0057] S1. Weigh accurately 15 g of ethanol and 20 g of phenol and place them in a container. Stir and dissolve until completely dissolved.

[0058] S2. Weigh accurately the remaining mass of deionized water and add it to the product in S2. Stir well.

[0059] Comparative Example 2:

[0060] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, the types of phenolic compounds added are different, and no surfactant is added, especially no composite organic acid is added; specifically:

[0061] S1. Weigh accurately 15 g of ethanol and 20 g of triclosan and place them in a container. Stir and dissolve until completely dissolved.

[0062] S2. Weigh accurately the remaining mass of deionized water and add it to the product in S2. Stir well.

[0063] Comparative Example 3:

[0064] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, no surfactant is added, especially no composite organic acid is added; specifically:

[0065] S1. Weigh accurately 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container. Stir and dissolve until completely dissolved.

[0066] S2. Weigh accurately the remaining mass of deionized water and add it to the product in S2. Stir well.

[0067] Comparative Example 4:

[0068] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, the added surfactant is different, especially the composite organic acid is not added; specifically:

[0069] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan, and place them in a container, and stir and dissolve until completely dissolved;

[0070] S2. Accurately weigh 10 g of ammonium lauryl sulfate, add it to the product in step S1, and stir well;

[0071] S3. Accurately weigh the remaining mass of deionized water, add it to the product in S2, and stir well.

[0072] Carry out physical and chemical detection, sterilization test, and stability test according to the publicly disclosed test methods.

[0073] Comparative Example 5:

[0074] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, the added surfactant is different, especially the composite organic acid is not added; specifically:

[0075] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan, and place them in a container, and stir and dissolve until completely dissolved;

[0076] S2. Accurately weigh 10 g of sorbitan fatty acid ester, add it to the product in step S1, and stir well;

[0077] S3. Accurately weigh the remaining mass of deionized water, add it to the product in S2, and stir well.

[0078] Carry out physical and chemical detection, sterilization test, and stability test according to the publicly disclosed test methods.

[0079] Comparative Example 6:

[0080] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, the added surfactant is different, especially the composite organic acid is not added; specifically:

[0081] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan, and place them in a container, and stir and dissolve until completely dissolved;

[0082] S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well;

[0083] S3. Accurately weigh the remaining mass of deionized water, add it to the product in S2, and stir well.

[0084] Perform physical and chemical tests, bactericidal tests, and stability tests according to the disclosed test methods.

[0085] Comparative Example 7:

[0086] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, an alkali was added; specifically:

[0087] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, and stir and dissolve until completely dissolved;

[0088] S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well;

[0089] S3. Accurately weigh 10 g of sodium hydroxide, add it to the product in step S2, and stir well;

[0090] S4. Accurately weigh the remaining mass of deionized water, add it to the product in S3, and stir well.

[0091] Perform physical and chemical tests, bactericidal tests, and stability tests according to the disclosed test methods.

[0092] Comparative Example 8:

[0093] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, an alkali was added; specifically:

[0094] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, and stir and dissolve until completely dissolved;

[0095] S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well;

[0096] S3. Accurately weigh 8 g of potassium hydroxide, add it to the product in step S2, and stir well;

[0097] S4. Accurately weigh the remaining mass of deionized water, add it to the product in S3, and stir well.

[0098] Perform physical and chemical tests, bactericidal tests, and stability tests according to the disclosed test methods.

[0099] Comparative Example 9:

[0100] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, a composite alkali was added; specifically:

[0101] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, and stir and dissolve until completely dissolved;

[0102] S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well;

[0103] S3. Accurately weigh 4 g of sodium hydroxide and 4 g of potassium hydroxide, add them to the product in step S2, and stir well;

[0104] S4. Accurately weigh the remaining mass of deionized water, add it to the product in S3, and stir well.

[0105] Carry out physical and chemical detection, bactericidal test, and stability test according to the publicly disclosed test methods.

[0106] Comparative Example 10:

[0107] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, inorganic acid was added, and the addition amount was converted equally according to the pH of the example; specifically:

[0108] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, and stir and dissolve until completely dissolved;

[0109] S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well;

[0110] S3. Accurately weigh 20 g of 5% dilute hydrochloric acid solution, add it to the product in step S2, and stir well;

[0111] S4. Accurately weigh the remaining mass of deionized water, add it to the product in S3, and stir well.

[0112] Carry out physical and chemical detection, bactericidal test, and stability test according to the publicly disclosed test methods.

[0113] Comparative Example 11:

[0114] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, the types of organic acids added are different; specifically:

[0115] S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, and stir and dissolve until completely dissolved;

[0116] S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well;

[0117] S3. Weigh accurately 20 g of citric acid, add it to the product in step S2, and stir well.

[0118] S4. Weigh accurately the remaining mass of deionized water, add it to the product in S3, and stir well.

[0119] Comparative Example 12:

[0120] The preparation method is the same as that of the example, except that: in the preparation process of this comparative example, no surfactant is added. Specifically:

[0121] S1. Weigh accurately 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, stir and dissolve until completely dissolved;

[0122] S2. Weigh accurately 10 g of L-malic acid and 10 g of citric acid, add them to the product in step S1, and stir well;

[0123] S3. Weigh accurately the remaining mass of deionized water, add it to the product in S2, and stir well.

[0124] Carry out physical and chemical detection, bactericidal test, and stability test according to the publicly disclosed test methods.

[0125] Detect the pH value of the above-mentioned comparative examples and examples according to the "Disinfection Technical Specification" 2002 edition, and visually compare the appearance properties of each comparative example and example. The results are as follows:

[0126] Table 1 Appearance properties and pH values of different examples and comparative examples

[0127] Group Appearance pH Control Example 1 Light yellow transparent liquid, without precipitation 6.57 Control Example 2 Transparent liquid, without precipitation 6.68 Control Example 3 Light yellow semi-transparent liquid, with precipitation 6.60 Control Example 4 Light yellow semi-transparent liquid, with precipitation 6.34 Control Example 5 Light yellow semi-transparent liquid, with precipitation 6.21 Control Example 6 Light yellow transparent liquid, with a small amount of precipitation 6.30 Control Example 7 Yellow turbid liquid, with a large amount of precipitation 13.82 Control Example 8 Yellow turbid liquid, with a large amount of precipitation 13.73 Control Example 9 Yellow turbid liquid, with a large amount of precipitation 13.80 Control Example 10 Light yellow transparent liquid, with a small amount of precipitation 1.13 Control Example 11 Light yellow transparent liquid, with a small amount of precipitation 0.94 Control Example 12 Yellow turbid liquid, stratified after standing 0.96 Example Light yellow transparent liquid, without precipitation 0.97

[0128] 2. Bactericidal effect

[0129] Referring to the "Disinfection Technical Specification" 2002 edition, using the suspension quantitative bactericidal test method, at 20 ± 1 °C, after diluting each example 1:128 times with standard hard water, carry out the killing test on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Mycobacterium chelonae, and Aspergillus niger in the presence of 3% organic interfering substances; carry out the inactivation test on poliovirus; carry out the fabric simulated field test on Escherichia coli and Staphylococcus aureus for 10 min, and calculate the killing logarithm. Among them, when the killing logarithm of E. coli, S. aureus, and P. aeruginosa ≥ 5.00, it is judged as qualified for disinfection; when the killing logarithm of C. albicans, M. chelonae, and A. niger ≥ 4.00, it is judged as qualified for disinfection; when the killing logarithm of the fabric simulated field test of E. coli and S. aureus ≥ 4.00; when the inactivation logarithm of poliovirus ≥ 4.00, it is evaluated as qualified for disinfection. See Table 2 for details.

[0130] Table 2 Detection of the killing effect of each example on microorganisms

[0131]

[0132] It can be seen from the data analysis of Table 1 and Table 2 that:

[0133] (1) According to the bactericidal effect of Table 2,

[0134] It can be seen from the comparison of Comparative Example 1, Comparative Example 2 and Comparative Example 3 that the combined use of phenol and triclosan has a better bactericidal effect than the individual use of phenol or triclosan;

[0135] And in Table 1: It can be seen from the comparison of Comparative Example 1, Comparative Example 2 and Comparative Example 3 that the addition of different phenolic compounds has a substantial impact on the appearance properties of the disinfectant. Among them, the combined use of phenol and triclosan affects the solubility in the ethanol system, and the system becomes neutral;

[0136] Therefore, the combined use of phenol and triclosan has a positive effect on the bactericidal effect, but has a negative effect on the "solubility of the phenolic compound composition in the ethanol system";

[0137] (2) And in Table 2: It can be seen from the comparison of Comparative Example 1 - Comparative Example 5 that the use of a dispersant has a certain impact on both the appearance properties and the bactericidal effect of the hand sanitizer, among which:

[0138] In terms of appearance properties:

[0139] Although a dispersant was added in Comparative Example 4 and Comparative Example 5, the "solubility of the phenolic compound composition in the ethanol system" was not improved;

[0140] Therefore, it can be seen from Comparative Example 6 that ammonium lauryl sulfate and sorbitan fatty acid ester as a composite dispersant can promote the solubility of the composite phenolic compound of phenol and triclosan in the ethanol system;

[0141] In terms of bactericidal effect:

[0142] Comparing Comparative Example 6 with Comparative Example 4 and Comparative Example 5, ammonium lauryl sulfate and sorbitan fatty acid ester as a composite dispersant can promote the bactericidal effect of the composite phenolic compound of phenol and triclosan;

[0143] (3) It can be seen from the comparison of the Examples with Comparative Example 7 - Comparative Example 9 that

[0144] Although the same equivalent of composite phenolic compound and composite dispersant was added to the system, due to the system pH becoming alkaline, a large amount of precipitation occurred in terms of the appearance properties of the disinfectant, and the bactericidal effect was poor;

[0145] (4) It can be seen from the comparison between the examples and Comparative Examples 10 - 12 that

[0146] The solubility of Comparative Example 10 and Comparative Example 11 is not much different from that of Comparative Example 6, and is worse than that of the examples of the present invention.

[0147] It can be seen from the comparison between the examples and Comparative Example 12 that although a composite organic acid composed of L - malic acid and citric acid is added, a large amount of precipitation occurs in terms of appearance properties.

[0148] Therefore, the composite organic acid composed of L - malic acid and citric acid itself has no significant promoting effect on the solubility of the "compound phenolic compounds of phenol and triclosan" in the ethanol system, but has a synergistic effect on promoting the solubility of the "compound phenolic compounds of phenol and triclosan" in the ethanol system with ammonium lauryl sulfate and sorbitan fatty acid ester as the composite dispersant.

[0149] 3. Stability test

[0150] Referring to Article 2.2.3 of the "Disinfection Technical Specification" 2002 Edition - Product Stability Test, the accelerated test method was used to detect the stability of each comparative example and the commercially available acidic phenolic disinfectant. After being placed at 37°C for 90 days, it was found that the appearance of Comparative Example 1 did not change, and the decline rate of the content of the bactericidal active ingredient was 9.03%; the appearance of Comparative Example 2 did not change, and the decline rate of the content of the bactericidal active ingredient was 8.61%; a small amount of precipitation appeared at the bottom of the bottle in Comparative Example 3, and the decline rate of the content of the bactericidal active ingredient was 13.38%; the color of Comparative Example 4 deepened, and the decline rate of the content of the bactericidal active ingredient was 7.96%; the color of Comparative Example 5 deepened, and the decline rate of the content of the bactericidal active ingredient was 7.73%; the color of Comparative Example 6 deepened, and the decline rate of the content of the bactericidal active ingredient was 6.10%; a large amount of precipitation appeared at the bottom of the bottle in Comparative Example 7, and the decline rate of the content of the main bactericidal ingredient was 30.94%; a large amount of precipitation appeared at the bottom of the bottle in Comparative Example 8, and the decline rate of the content of the main bactericidal ingredient was 21.17%; a large amount of precipitation appeared at the bottom of the bottle in Comparative Example 9, and the decline rate of the content of the main bactericidal ingredient was 24.81%; the appearance of Comparative Example 10 did not change, and the decline rate of the content of the main bactericidal ingredient was 4.37%; the appearance of Comparative Example 11 did not change, and the decline rate of the content of the main bactericidal ingredient was 5.60%; the appearance of Comparative Example 12 did not change, and the decline rate of the content of the main bactericidal ingredient was 12.71%; the appearance of the example did not change, and the decline rate of the content of the main bactericidal ingredient was 3.35%; the color of the commercially available acidic phenolic disinfectant deepened and turned red, and the decline rate of the content of the main bactericidal ingredient was 9.40%.

[0151] Table 3 Stability test results of each example and a commercially available acidic phenolic disinfectant

[0152]

[0153]

[0154] It can be seen from the data analysis in Table 3 that:

[0155] It can be seen from the comparison of Comparative Example 1, Comparative Example 2 and Comparative Example 3 that the mixed use of phenol and triclosan is inferior to the separate storage stability of phenol or triclosan in terms of storage stability.

[0156] It can be seen from the comparison of Comparative Example 4, Comparative Example 5 and Comparative Example 6 that the mixed use of ammonium lauryl sulfate and sorbitan fatty acid ester is superior to the separate use of ammonium lauryl sulfate or sorbitan fatty acid ester in terms of storage stability; however, whether added alone or as a mixture, it will cause the color of the disinfectant solution to deepen after storage stability.

[0157] It can be seen from the comparison of the Examples with Comparative Example 7, Comparative Example 8 and Comparative Example 9 that a large amount of precipitation is generated in the alkaline system disinfectant solution after storage stability, and the main bactericidal components of each comparative example exceed the requirements for the stability of disinfectant solutions in the "Disinfection Technical Specifications" 2002 edition.

[0158] It can be seen from the comparison of the Examples with Comparative Example 10 and Comparative Example 11 that the mixed use of L-citric acid and malic acid is superior to the mixed use of L-citric acid or malic acid in terms of storage stability.

[0159] It can be seen from the comparison of the Examples with Comparative Example 12 that under the condition of the mixed use of L-citric acid and malic acid, the sample added with the mixture of ammonium lauryl sulfate and sorbitan fatty acid ester is superior to the sample not added with the mixture of ammonium lauryl sulfate and sorbitan fatty acid ester.

[0160] 4. Storage stability of the dilution

[0161] The Examples, each comparative example and the commercially available acidic phenolic disinfectant were diluted with hard water at a ratio of 1:128. The bactericidal effects on Staphylococcus aureus and Aspergillus niger were detected respectively after being sealed and stored for 7 days, 14 days and 20 days. 3% organic interferent was used and the action time was ten minutes.

[0162] Table 4 Detection of the bactericidal effects of each Example and the commercially available acidic phenolic disinfectant after dilution and storage for a certain period of time

[0163]

[0164]

[0165] Note: NA means that the number of colonies on the plate is too many to be counted.

[0166] It can be seen from the data analysis in Table 4 that:

[0167] The commercially available acidic phenolic disinfectant is sealed and stored after dilution with hard water. The logarithmic reduction value of Staphylococcus aureus after 7 days is 3.01, and the logarithmic reduction value of Aspergillus niger is 1.64; after 14 days, the logarithmic reduction value of Staphylococcus aureus is 0.81, and the logarithmic reduction value of Aspergillus niger is 0.62; after 20 days, the logarithmic reduction value of Staphylococcus aureus is 0.37, and the logarithmic reduction value of Aspergillus niger is 0.11;

[0168] In Comparative Examples 1-9, in most cases, the number of colony forming units on the plate was too large to be counted.

[0169] Comparing Comparative Examples 10 and 11 with the examples of the present invention, Comparative Examples 10 and 11 only had a certain sterilization effect on Staphylococcus aureus and Aspergillus niger after 7 days, while after 14 days and 20 days, the number of colony forming units on the plate was too large to be counted;

[0170] In the examples of the present invention, after dilution and sealed storage for 7 days, 14 days and 20 days, the logarithmic reduction value of Staphylococcus aureus can be ≥5.00, and the logarithmic reduction value of Aspergillus niger can be ≥4.00;

[0171] 5. Toxicology test

[0172] The examples should comply with the Technical Requirements for Hygienic Safety Evaluation of Disinfection Products WS628 and the General Requirements for Disinfectants for General Object Surfaces GB27952, as shown in Table 5 specifically.

[0173] Table 5 Toxicology test detection

[0174] Item Toxicity Evaluation Acute Oral Toxicity Test Belongs to practically non-toxic Single Complete Skin Irritation Test Belongs to mild irritation or non-irritating One mutagenicity (mouse bone marrow polychromatic erythrocyte micronucleus test) No mutagenicity

[0175] In summary:

[0176] (1) In terms of appearance and properties:

[0177] As a composite dispersant, ammonium lauryl sulfate and sorbitan fatty acid ester can promote the solubility of the compound phenolic compounds of phenol and triclosan in the ethanol system;

[0178] In terms of bactericidal effect:

[0179] As a composite dispersant, ammonium lauryl sulfate and sorbitan fatty acid ester can promote the bactericidal effect of the compound phenolic compounds of phenol and triclosan;

[0180] (2) The composite organic acid composed of L-malic acid and citric acid itself has a synergistic effect on promoting the solubility of "the compound phenolic compounds of phenol and triclosan" in the ethanol system by "ammonium lauryl sulfate and sorbitan fatty acid ester as a composite dispersant".

[0181] (3) In the present invention, one or more phenolic compounds are dissolved in deionized water, and a dispersant and an acidic substance are added to enhance the bactericidal effect and maintain the stability of the phenolic compounds. Double-layer packaging is carried out and irradiation is performed to ensure strict sterility of the product. The sample is a concentrated solution, which is convenient for transportation and storage. It can be diluted with hard water at a ratio of 1:128 for use, and the disinfection effect can meet the standard within 20 days when stored sealed after dilution.

Claims

1. A concentrated acidic phenolic disinfectant, characterized in that It includes compound phenolic compounds, dispersants, surfactants, compound organic acids and the balance of water; The compound phenolic compounds are a mixture of phenol and triclosan; The compound organic acids are a mixture of L-malic acid and citric acid; The dispersant is ethanol; the surfactant is a mixture of ammonium lauryl sulfate and sorbitan fatty acid ester; The preparation method of the acidic phenolic disinfectant includes: S1. Accurately weigh 15 g of ethanol, 10 g of phenol, and 10 g of triclosan and place them in a container, and stir and dissolve until completely dissolved; S2. Accurately weigh 5 g of ammonium lauryl sulfate and 5 g of sorbitan fatty acid ester, add them to the product in step S1, and stir well; S3. Accurately weigh 10 g of L-malic acid and 10 g of citric acid, add them to the product in step S2, and stir well; S4. Accurately weigh the remaining mass of deionized water, add it to the product in S3, and stir well.

2. The concentrated acidic phenolic disinfectant according to claim 1, characterized in that By weight percentage, the components of the acidic phenolic disinfectant are: 0.1%-40% of compound phenolic compounds, 0.1-40% of dispersants, 0.1%-25% of compound organic acids, and the balance is water.

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