A composition for promoting the robustness and stress resistance of wheat

Abstract

The present invention belongs to the technical field of pesticides, and specifically relates to a composition for promoting the robustness and stress resistance of wheat. A composition for promoting the robustness and stress resistance of wheat, the active ingredients of which are compounded by oligosaccharins and flutriafol-activated ester, hydromycin or piperlongumineamide in a mass ratio of 1-100:100-1. The present invention screens and compounded flutriafol-activated ester, hydromycin or piperlongumineamide which has a synergistic effect with oligosaccharins. Among them, oligosaccharins regulate and promote plant growth, promote robustness, improve stress resistance, improve crop quality and increase crop yield. At the same time, the compound composition has a synergistic effect on fungal diseases such as wheat powdery mildew, can improve the control effect, reduce the amount of pesticide application and reduce pesticide residues. Thus, when applying the compound composition of the present invention, it can not only promote the robustness of wheat plants and improve their stress resistance, but also effectively control fungal diseases such as wheat powdery mildew.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a composition for promoting the robustness and stress resistance of wheat. Background Art

[0002] Oligochitosan, also known as agricultural special chitosan oligosaccharide, is a polysaccharide natural product extracted from the shells of marine organisms. It is obtained by degrading chitin to chitosan and then further degrading. Oligochitosan is non-toxic and non-polluting, and has dual functions of drug efficacy and fertilizer efficacy. Oligochitosan can stimulate the plant's own immune response, activate plant cells, regulate and promote plant growth, improve stress resistance, enhance the cold resistance, drought resistance and waterlogging resistance of crops, increase the seed germination rate, strengthen seedlings, promote, improve the absorption and utilization rate of fertilizers and water by plants, enhance the stress resistance of crops, improve the crop quality, and increase the crop yield. In addition, as a bactericide with disease-resistant effects, oligochitosan has a wide bactericidal spectrum and is effective against diseases caused by a variety of fungi, bacteria and viruses.

[0003] Fludioxonil-activated ester (abbreviation: FBT) is a new type of plant-induced disease-resistant agent with independent intellectual property rights jointly developed by East China University of Science and Technology and the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences. It belongs to the BTH analog. Research has found that fludioxonil-activated ester does not directly kill pathogens, but exerts its effect by inducing systemic acquired resistance in plants, and it belongs to a new type of pesticide. Application No. CN201210372076.8 discloses that when fludioxonil-activated ester is compounded with carbendazim, thiophanate-methyl, prochloraz or flusilazole, etc., it has a control effect on various plant diseases such as powdery mildew, scab, rice blast, root rot, brown spot, scab and stem blight.

[0004] Hydromycin is an antibiotic bactericide prepared by fermentation and culture of actinomycetes. Its bactericidal mechanism is to block the synthesis of bacterial proteins and the replication of DNA, thus having a super eradication effect and having good control effects on fungal and bacterial diseases on crops. Application No. CN201410649217.5 discloses a bactericidal composition of succinic acid copper and hydromycin, which can prevent plant diseases such as canker, angular leaf spot, anthracnose, false smut, powdery mildew, root rot and virus disease.

[0005] Piperlongumine is also known as piperlonguminine, CAS: 20069-09-4, molecular formula: C 17 H 19 NO5, molecular weight: 317.34, and the structural formula is as follows:

[0006]

[0007] Piper longum amide belongs to the amide alkaloid compounds and is mainly found in the roots of Piper longum, a plant of the Piperaceae family. Studies have shown that piper longum amide is a chemical substance with great medicinal value, especially the anti-tumor activity of piper longum amide has been the most widely studied. In addition, studies have shown that piper longum amide compounds can prevent and treat plant fungal diseases. Application No. CN202111143459.3 discloses that piper longum amide can be used to prevent and treat banana wilt pathogens, tomato gray mold pathogens, wheat fusarium wilt pathogens, apple ring rot pathogens, tobacco red star pathogens, litchi downy mildew pathogens, corn leaf blight pathogens, and rubber tree coryneformis leaf blight pathogens.

[0008] Wheat powdery mildew is a worldwide fungal disease caused by the wheat-specific form of Blumeria graminis. It is the main disease in wheat production in the Yangtze River region of my country. In recent years, wheat powdery mildew has not only occurred early in the Yangtze River region but also recurred frequently, seriously hindering the growth and development of wheat, causing a decrease in the number of wheat tillers, ears, and grains per ear, and a decrease in thousand-grain weight, which has seriously affected wheat yields. Chemical agents are still the most economical and effective means of preventing and controlling wheat powdery mildew, but with the continuous use of agents, their effectiveness in preventing and controlling wheat powdery mildew has gradually declined.

[0009] The long-term continuous use of a single active ingredient of pesticides can easily lead to the risk of pathogenic bacteria developing resistance and increasing the risk of dosage, thereby increasing the cost of prevention and control and the environmental load. By compounding or mixing different active ingredients of pesticides to screen for compound compositions with synergistic effects, the control effect on target pathogens can be improved, thereby not only reducing the amount of pesticides used, reducing the cost of prevention and control and the risk of resistance, but also reducing the potential environmental load and extending the service life of pesticides.

[0010] So far, there are no reports on the combination of amino oligosaccharides with fluazole activated ester, hydramycin or piperlongum amide.

[0011] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0012] The purpose of the present invention is to provide a wheat robustness-promoting and stress-resistant composition to solve the problem that the effect of the agent on preventing and controlling wheat powdery mildew gradually decreases with the continuous use of the agent.

[0013] To achieve the above object, the present invention provides the following technical solutions:

[0014] A wheat robustness-promoting and stress-resistance composition, wherein the effective components are prepared by compounding amino oligosaccharides with fluazole activated ester, hydramycin or piperlongum amide in a mass ratio of 1-100:100-1.

[0015] Preferably, the mass ratio of the oligosaccharins to fludioxonil is 1-8:24-1.

[0016] Preferably, the mass ratio of the oligosaccharins to hydromycin is 1-16:40-1.

[0017] Preferably, the mass ratio of the oligosaccharins to piperlongumine is 1-10:27-1.

[0018] The present invention also provides the application of the composition for promoting the growth and resistance of wheat in the prevention and control of powdery mildew of wheat.

[0019] The present invention also provides a bactericide, which comprises the composition for promoting the growth and resistance of wheat and auxiliary components permitted to be added in pesticide science.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The composition for promoting the growth and resistance of wheat in the present invention is screened and compounded with fludioxonil, hydromycin or piperlongumine which has a synergistic effect with oligosaccharins. Among them, oligosaccharins regulate and promote plant growth, promote robustness, improve stress resistance, improve crop quality and increase crop yield; at the same time, the compound composition has a synergistic effect on fungal diseases such as powdery mildew of wheat, can improve the control effect, reduce the application amount of pesticides and reduce pesticide residues. Thus, when applying the compound composition of the present invention, it can not only promote the robustness of wheat plants and improve their stress resistance, but also effectively control fungal diseases such as powdery mildew of wheat.

[0022] (2) The compounding of oligosaccharins with fludioxonil, hydromycin or piperlongumine in the present invention can reduce the risk of drug resistance of using a single active ingredient, and is highly efficient and safe at the same time. Specific Embodiments

[0023] The technical solutions of the present invention will be described clearly and completely below. Obviously, the described embodiments are a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the scope of protection of the present invention.

[0024] Examples : Indoor bioactivity determination of oligosaccharins compounding

[0025] 1. Test crops: Seeds of "Zhengmai 9023" wheat, potted and cultured until the 2-3 true leaf stage, 10 plants per pot, numbered for standby.

[0026] 2. Test agents

[0027] 85% Oligochitosan (Qingdao Zhongda Agricultural Technology Co., Ltd.)

[0028] 98% Flutriafol Activated Ester (Nantong Taihe Chemical Co., Ltd.)

[0029] 99% Blasticidin-S (Hubei Youlunfeng Biotechnology Co., Ltd.)

[0030] 98% Piperlongumine (Zhengzhou Huiju Chemical Co., Ltd.)

[0031] 3. Test Method (Refer to NY / T 1156.4-2008 "Guidelines for Pesticide Bioassay in the Laboratory - Fungicides - Part 4: Potted Plant Test for Controlling Powdery Mildew of Wheat")

[0032] 3.1 Preparation of Medicaments: Dissolve the technical material with dimethyl sulfoxide and dilute with 0.1% Tween-80 aqueous solution to prepare single-agent stock solutions respectively. Set multiple groups of ratios, and set 5 series of mass concentration gradients for each single agent and the mixed agents of each group ratio;

[0033] 3.2 Inoculation Treatment: Evenly shake off the fresh spores of powdery mildew fungus produced within 24 hours on the diseased wheat leaves and inoculate them onto the test wheat seedlings;

[0034] 3.3 Medicament Treatment: After 24 hours, evenly spray the liquid medicine on the leaves of the test wheat seedlings until they are all wet. There are 3 pots for each treatment, and set a treatment containing only 0.1% Tween-80 aqueous solution as the blank control; After natural air drying, move them to a constant temperature room and continue to culture under the conditions of temperature 22°C, light:dark = 16h:8h.

[0035] 3.4 Investigation and Calculation: When the disease leaf rate of the blank control reaches more than 80%, investigate the disease incidence of each treatment according to the grading standard in Table 1 below. According to the investigation data, calculate the disease index and control effect of each treatment. Among them, the grading standard is shown in Table 1.

[0036] Table 1 Disease Grade Evaluation Standard

[0037]

[0038]

[0039]

[0040] 3.5 Data Analysis: Use DPS software for data statistical analysis to obtain the virulence regression equation and the virulence EC 50 value of the medicament against the target pathogen, and calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method.

[0041] 3.6. Efficacy evaluation: The synergistic effect of the agents was evaluated based on the calculated co-toxicity coefficient (CTC). An antagonistic effect was indicated when CTC ≤ 80, an additive effect when 80 < CTC < 120, and a synergistic effect when CTC ≥ 120. The results are shown in Table 2-4.

[0042] Table 2 Indoor biological activity of the compound of oligosaccharins and fludioxonil against Blumeria graminis f. sp. tritici

[0043] Names and ratios of medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Amino-oligosaccharins 24.3465 100.0000 -- -- Fludioxonil 12.8604 189.3137 -- -- Amino-oligosaccharins 1: Fludioxonil 24 9.4709 257.0664 185.7412 138.4003 Amino-oligosaccharins 1: Fludioxonil 16 6.7492 360.7316 184.0600 195.9859 Amino-oligosaccharins 1: Fludioxonil 8 10.1522 239.8150 179.3900 133.6836 Amino-oligosaccharins 1: Fludioxonil 4 11.4130 213.3225 171.4510 124.4219 Amino-oligosaccharins 1: Fludioxonil 1 13.9674 174.3095 144.6569 120.4986 Amino-oligosaccharins 4: Fludioxonil 1 16.4695 147.8278 117.8627 125.4237 Amino-oligosaccharins 8: Fludioxonil 1 18.0856 134.6181 109.9237 122.4650

[0044] As can be seen from Table 2, after the compounding of oligosaccharins and fludioxonil, the co-toxicity coefficients against Blumeria graminis f. sp. tritici were all greater than 120 in the mass ratio range of 1-8:24-1, showing a synergistic effect.

[0045] Table 3 Indoor biological activity of the compound of oligosaccharins and hydromycin against Blumeria graminis f. sp. tritici

[0046] Names and ratios of medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Amino-oligosaccharins 24.3465 100.0000 -- -- Hydramycin 56.6718 42.9605 -- -- Amino-oligosaccharins 1: Hydramycin 40 33.7707 72.0936 44.3517 162.5496 Amino-oligosaccharins 1: Hydramycin 30 28.7462 84.6947 44.8005 189.0485 Amino-oligosaccharins 1: Hydramycin 20 24.6180 98.8971 45.6767 216.5156 Amino-oligosaccharins 1: Hydramycin 16 41.4367 58.7559 46.3158 126.8593 Amino-oligosaccharins 1: Hydramycin 8 34.4065 70.7613 49.2982 143.5373 Amino-oligosaccharins 1: Hydramycin 4 21.4543 113.4807 54.3684 208.7255 Amino-oligosaccharins 1: Hydramycin 1 27.3368 89.0613 71.4803 124.5956 Amino-oligosaccharins 4: Hydramycin 1 17.1931 141.6062 88.5921 159.8407 Amino-oligosaccharins 8: Hydramycin 1 19.8125 122.8845 93.6623 131.1996 Amino-oligosaccharins 16: Hydramycin 1 14.4679 168.2794 96.6447 174.1217

[0047] As can be seen from Table 3, after the compounding of oligosaccharins and hydromycin, the co-toxicity coefficients against Blumeria graminis f. sp. tritici were all greater than 120 in the mass ratio range of 1-16:40-1, showing a synergistic effect.

[0048] Table 4 Indoor biological activity of the compound of oligosaccharins and piperlongumine against Blumeria graminis f. sp. tritici

[0049] Names and ratios of medicaments <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Amino-oligosaccharins 24.3465 100.0000 -- -- Piperlongumine 40.2761 60.4490 -- -- Amino-oligosaccharins 1: Piperlongumine 27 31.5917 77.0661 61.8615 124.5784 Amino-oligosaccharins 1: Piperlongumine 18 29.6090 82.2267 62.5306 131.4983 Amino-oligosaccharins 1: Piperlongumine 9 23.1608 105.1194 64.4041 163.2185 Amino-oligosaccharins 1: Piperlongumine 7 20.3543 119.6135 65.3929 182.9153 Amino-oligosaccharins 1: Piperlongumine 3 16.2335 149.9769 70.3368 213.2269 Amino-oligosaccharins 1: Piperlongumine 1 25.1005 96.9961 80.2245 120.9058 Amino-oligosaccharins 3: Piperlongumine 1 13.7350 177.2588 90.1123 196.7089 Amino-oligosaccharins 7: Piperlongumine 1 8.9678 271.4880 95.0561 285.6081 Amino-oligosaccharins 10: Piperlongumine 1 5.5692 437.1633 96.4045 453.4680

[0050] As can be seen from Table 4, after the compounding of oligosaccharins and piperlongumine, the co-toxicity coefficients against Blumeria graminis f. sp. tritici were all greater than 120 in the mass ratio range of 1-10:27-1, showing a synergistic effect.

[0051] In summary, when oligosaccharins are compounded with fludioxonil, hydromycin or piperlongumine, they have a synergistic effect on wheat powdery mildew, which can improve the control effect on wheat powdery mildew, thereby reducing the amount of pesticide application, reducing pesticide residues and lowering the control cost.

[0052] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many changes and variations are possible in light of the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention as well as various different selections and changes. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A composition for promoting the robustness and stress resistance of wheat, characterized in that, Its active ingredient is composed of chitosan oligosaccharide and fludioxonil or hygromycin B; among them, the mass ratio of the chitosan oligosaccharide to the fludioxonil is 1-8:24-1; the mass ratio of the chitosan oligosaccharide to the hygromycin B is 1-16:40-1.

2. Use of the composition for promoting the growth and robustness and stress resistance of wheat according to claim 1 in the prevention and control of powdery mildew of wheat.

3. A fungicide, characterized in that, Comprising the composition for promoting the growth and robustness and stress resistance of wheat according to claim 1.

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