A pesticide composition containing fluxapyroxad and boscalid, a preparation method thereof, and uses thereof

Abstract

The present invention relates to a pesticide composition containing fludioxonil and boscalid, a preparation method thereof and uses. The 90% fludioxonil technical and 95% boscalid technical are respectively formulated into stock solutions of 1×10 4 mg / L with methanol and stored at 4°C. The above stock solutions are respectively formulated into liquid medicines containing 10 - 40 mg / L of fludioxonil and 50 - 200 mg / L of boscalid with 0.1% Tween 20. The liquid medicines containing 20 - 80 mg / L of fludioxonil and 100 - 400 mg / L of boscalid are mixed in equal volumes, and the resulting composition contains 10 - 40 mg / L of fludioxonil and 50 - 200 mg / L of boscalid. The present invention can prevent and control the tobacco seed rot caused by Cladosporium, has strong bactericidal activity and is environmentally friendly.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a pesticide composition containing fludioxonil and boscalid, a preparation method of the pesticide composition containing fludioxonil and boscalid, and also relates to the use of the pesticide composition containing fludioxonil and boscalid in preventing and controlling tobacco seed rot caused by Cladosporium cladosporioides. Background Art

[0002] Tobacco seed rot caused by Cladosporium cladosporioides Cladosporium cladosporioides (Fresen.) G.A. de Vries is a fungal disease that easily occurs during the harvesting and storage periods of tobacco seeds. It mainly occurs during the seed storage period, usually during or after seed germination. The pathogen spores germinate to produce dark brown hyphae, and the hyphae infect germinated and post-germination tobacco seeds or tobacco seedlings, quickly producing black spores and further spreading to cause harm. Impurities remaining in tobacco seeds during the harvesting and storage periods, high humidity in the storage environment, and incomplete disinfection can all cause seed rot and also pose a threat during the seed germination period. This disease is characterized by being hidden and highly harmful. In severe cases, the incidence rate can reach 100%, often resulting in a decline in the quality of tobacco seeds, a decrease in germination rate, and a reduction in the quality of tobacco seedlings, thus damaging the interests of tobacco farmers. Cladosporium cladosporioides belongs to the class Hyphomycetes, the order Miniliales, and the family Moniliaceae of fungi. It produces a large number of conidia during its growth process, and the colors of its colonies include olive green, coffee black, etc.

[0003] Currently, there is relatively little research on the prevention and control of plant diseases caused by Cladosporium cladosporioides. There is only research on Cladosporium cladosporioides of bananas. Zhang Xin et al. evaluated the virulence of 13 fungicides, namely difenoconazole, thiophanate-methyl, dimethomorph, pyraclostrobin, flusilazole, procymidone, prochloraz, tebuconazole, myclobutanil, mancozeb, triadimefon, Bordeaux mixture, and kasugamycin, against Cladosporium cladosporioides of bananas and found that difenoconazole, thiophanate-methyl, dimethomorph, pyraclostrobin, and flusilazole have certain activities. There are no reported patents on the prevention and control of plant diseases caused by Cladosporium cladosporioides. In summary, there is an urgent need to find a solution to tobacco seed rot to solve the disease problems of seeds during storage and processing caused by Cladosporium cladosporioides.

[0004] Chemical control is the most economical and effective main measure among many control methods for plant disease control. Therefore, searching for highly efficient and safe pesticide combinations for seed treatment during seed disinfection or seed coating can effectively control the mildew of tobacco seeds, improve the control effect, reduce disease losses, and has broad application prospects. At the same time, it is also the need for the safe and sustainable development of tobacco. Fludioxonil belongs to benzothiazole fungicides, which act on the spindle in the mitosis of dry pathogens and kill fungi by affecting cell division. Boscalid is a mitochondrial respiration inhibitor, a succinate dehydrogenase inhibitor (SDHI), which acts by inhibiting succinate coenzyme Q reductase (also known as complex II) on the mitochondrial electron transport chain. Currently, it is mainly used for the prevention and control of various vegetable diseases such as gray mold of tomatoes and strawberries. At present, there is no report on its compound application for the control of seed diseases. Summary of the Invention

[0005] The purpose of the present invention is to overcome the above-mentioned drawbacks and provide a pesticide composition containing fludioxonil and boscalid, which can prevent and control the mildew disease caused by Cladosporium sphaerospermum during the storage and processing of tobacco seeds, has strong bactericidal activity, is environmentally friendly, and has low cost.

[0006] Another purpose of the present invention is to provide a preparation method of the pesticide composition containing fludioxonil and boscalid.

[0007] Another purpose of the present invention is to provide the use of the pesticide composition containing fludioxonil and boscalid in preventing and controlling the mildew disease caused by Cladosporium sphaerospermum during the storage and processing of tobacco seeds.

[0008] A pesticide composition containing fludioxonil and boscalid of the present invention, wherein: the content of fludioxonil is 10 - 40 mg / L, and the content of boscalid is 50 - 200 mg / L.

[0009] For the above-mentioned pesticide composition containing fludioxonil and boscalid, preferably, the content of fludioxonil is 20 - 30 mg / L, and the content of boscalid is 60 - 150 mg / L.

[0010] For the above-mentioned pesticide composition containing fludioxonil and boscalid, wherein: fludioxonil is a 90% mass concentration pesticide technical, and boscalid is a 95% mass concentration pesticide technical.

[0011] A preparation method of a pesticide composition containing fludioxonil and boscalid of the present invention includes the following steps:

[0012] (1) Respectively dissolve 90% mass concentration fludioxonil technical and 95% mass concentration boscalid technical in methanol to prepare stock solutions of 1×10 4 mg / L, store at 4°C at low temperature for later use;

[0013] (2) Prepare the above mother liquors into medicated solutions containing 20 - 80 mg / L of fludioxonil and 100 - 400 mg / L of boscalid respectively with 0.1% Tween 20.

[0014] (3) Mix the above medicated solutions containing 20 - 80 mg / L of fludioxonil and 100 - 400 mg / L of boscalid in equal volumes. The prepared composition contains 10 - 40 mg / L of fludioxonil and 50 - 200 mg / L of boscalid.

[0015] Use of a pesticidal composition containing fludioxonil and boscalid in controlling tobacco seed rot.

[0016] Compared with the prior art, the present invention has obvious beneficial effects. From the above technical solutions, it can be seen that in the present invention, fludioxonil and boscalid are respectively prepared into mother liquors with methanol. The two base materials of fludioxonil and boscalid do not undergo chemical reactions. When in use, they are diluted with sterile water containing 0.1% Tween 20. The composition is directly sprayed on the tobacco seeds for impurity removal or sprayed on the tobacco seeds to be coated. The present invention aims at pathogens such as Blastomyces on tobacco seeds, can inhibit the infection of pathogenic bacteria, inhibit the production and germination of pathogenic bacteria spores, and reduce the number of re - infection cycles of diseases. It has obvious prevention and control effects, significantly improves the bactericidal effect compared with single agents, can reduce the drug use cost, and reduce environmental pollution; it can reduce the potential risk of drug resistance of pathogenic bacteria to fludioxonil and boscalid, and has a dual - effect treatment on other pathogenic bacteria that can cause tobacco seed rot. In - vivo tests show that the composition of fludioxonil and boscalid has good control effects on tobacco seed rot and is safe for the germination and development of tobacco seeds. The control effect of tobacco seed rot is better than that of fludioxonil alone and boscalid alone. Specific implementation mode

[0017] Example 1

[0018] A preparation method of a pesticidal composition containing fludioxonil and boscalid, comprising the following steps:

[0019] (1) Respectively prepare mother liquors of 90% fludioxonil technical material and 95% boscalid technical material with methanol at a concentration of 1×10 4 mg / L, and store them at 4°C for later use;

[0020] (2) Prepare the above mother liquors into a medicated solution containing 80 mg / L of fludioxonil and a medicated solution containing 180 mg / L of boscalid respectively with 0.1% Tween 20.

[0021] (3) Mix the above-mentioned fluxapyroxad liquid medicine and boscalid liquid medicine in equal volumes. The prepared composition contains 40 mg / L of fluxapyroxad and 50 mg / L of boscalid.

[0022] Example 2

[0023] A preparation method of a pesticide composition containing fluxapyroxad and boscalid, comprising the following steps:

[0024] (1) Respectively prepare stock solutions of 90% fluxapyroxad technical material and 95% boscalid technical material at 1×10 4 mg / L with methanol, and store them at 4°C for later use;

[0025] (2) Respectively prepare a liquid medicine containing 20 mg / L of fluxapyroxad and a liquid medicine containing 100 mg / L of boscalid from the above-mentioned stock solutions with 0.1% Tween 20;

[0026] (3) Mix the above-mentioned fluxapyroxad liquid medicine and boscalid liquid medicine in equal volumes. The prepared composition contains 10 mg / L of fluxapyroxad and 200 mg / L of boscalid.

[0027] Example 3

[0028] A preparation method of a pesticide composition containing fluxapyroxad and boscalid, comprising the following steps:

[0029] (1) Respectively prepare stock solutions of 90% fluxapyroxad technical material and 95% boscalid technical material at 1×10 4 mg / L with methanol, and store them at 4°C for later use;

[0030] (2) Respectively prepare a liquid medicine containing 60 mg / L of fluxapyroxad and a liquid medicine containing 200 mg / L of boscalid from the above-mentioned stock solutions with 0.1% Tween 20.

[0031] (3) Mix the above-mentioned fluxapyroxad liquid medicine and boscalid liquid medicine in equal volumes. The prepared composition contains 30 mg / L of fluxapyroxad and 100 mg / L of boscalid.

[0032] Test example: Test on the control of tobacco seed rot by the pesticide compositions of fluxapyroxad and boscalid prepared in Examples 1-3

[0033] Collect mature tobacco seeds (Yunyan 87), and perform threshing, impurity removal, washing and drying according to the normal procedure.

[0034] Treatment with the pesticide composition containing fluxapyroxad and boscalid: Use a sprayer to evenly spray the composition liquid medicine in the above examples onto the naked tobacco seeds. After the liquid medicine dries, store them according to the normal procedure.

[0035] Control treatment: Use a sprayer to evenly spray clear water onto the naked tobacco seeds, and after air-drying, store them according to the normal procedure.

[0036] After seed treatment, take 300 seeds from each treatment respectively, and use the filter paper method to detect the seed-borne bacteria rate. Calculate the control effect of each treatment of the pharmaceutical composition on seed rot according to the inhibition rate of the seed-borne bacteria rate.

[0037] The test results of the control effect of tobacco seed rot show that a pesticide combination containing fludioxonil and boscalid of the present invention can effectively control tobacco seed rot, and the disease control effect reaches more than 95.74%.

[0038] Table 1 Control effect of the pesticide compositions in Examples 1-3 on tobacco seed rot

[0039]

[0040] The above is only a preferred embodiment of the present invention, and does not impose any form of limitation on the present invention. Any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. Use of a pesticide composition containing fludioxonil and boscalid in controlling tobacco seed rot caused by Cladosporium cladosporioides.

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