A skin repair essence and its preparation method

Abstract

This application relates to the field of cosmetic technology, and more specifically, to a skin repair essence and its preparation method. A skin repair essence includes functional polypeptide liposomes. The preparation steps for the functional polypeptide liposomes are as follows: S1: Mixing whey protein powder, bovine colostrum powder, and water to obtain a mixed protein aqueous solution; ultrasonically treating the mixed protein aqueous solution to obtain an ultrasonically treated mixed protein aqueous solution; S2: Adjusting the pH and temperature of the ultrasonically treated mixed protein aqueous solution obtained in S1, and then adding trypsin for enzymatic hydrolysis to obtain an enzymatic hydrolysis product; S3: Centrifuging the enzymatic hydrolysis product obtained in S2 and collecting the supernatant; boiling, dialyzing, purifying, filtering, and freeze-drying the supernatant to obtain the functional polypeptide; S4: Mixing the liposomes and the functional polypeptide obtained in S3, and then filtering and drying to obtain the functional polypeptide liposomes; In S3, the molecular weight of the functional polypeptide is 4-5 kDa.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more specifically, to a skin repair essence and its preparation method. Background Technology

[0002] The skin is an important and largest organ covering the entire body surface. It is the protector of the body's internal organs and tissues, and also an effector of internal organs, the mind, and the surrounding environment, playing a particularly important role in human health.

[0003] However, due to external environmental factors, as well as individual factors such as mental state and age, the skin's natural barrier can become compromised, leading to skin aging, dryness, sagging, and wrinkles. Therefore, timely repair of the skin barrier is crucial for improving skin laxity and wrinkles.

[0004] Although there are many cosmetics on the market that claim to repair the skin barrier, the absorption and penetration capacity of these products decreases as the skin ages, resulting in low utilization rates. Therefore, most skin barrier repair cosmetics on the market are not very effective in repairing the skin barrier. Summary of the Invention

[0005] To improve the repair effect on the skin barrier, this application provides a skin repair essence and its preparation method.

[0006] In the first aspect, this application provides a skin repair essence, which adopts the following technical solution:

[0007] A skin repair serum, comprising the following components by weight percentage:

[0008] Mannitol 32-35%;

[0009] Functional polypeptide liposomes 22-26%;

[0010] Hydrogenated lecithin 14-18%;

[0011] Palmitoyl oligopeptide 4.4-7.2%;

[0012] Yeast extract 0.3-0.6%;

[0013] Water is the remainder;

[0014] The functional polypeptide liposomes include the following preparation steps:

[0015] S1: Mix whey protein powder, bovine colostrum powder and water to obtain a mixed protein aqueous solution. Sonicate the mixed protein aqueous solution to obtain a sonicated mixed protein aqueous solution.

[0016] S2: Adjust the pH and temperature of the ultrasonically treated mixed protein aqueous solution obtained in S1, and then add trypsin for enzymatic hydrolysis to obtain the enzymatic hydrolysis product;

[0017] S3: Centrifuge the enzymatic hydrolysis product obtained in S2 and collect the supernatant; boil the supernatant, dialyze, purify, filter, freeze dry, and the functional peptide is obtained.

[0018] S4: After mixing the liposomes and the functional peptides obtained in S3, the mixture is filtered and dried to obtain functional peptide liposomes.

[0019] In S3, the molecular weight of the functional peptide is 4-5 kDa.

[0020] By adopting the above technical solution, firstly, the mixture of whey protein powder, bovine colostrum powder, and water is pre-treated with ultrasound, which promotes moderate denaturation of the proteins in the whey protein powder and bovine colostrum powder. This facilitates the enzymatic hydrolysis by trypsin in step S2, improving the efficiency of the enzymatic hydrolysis of the mixed protein aqueous solution and increasing the activity of the hydrolysate. Simultaneously, the functional peptide has a molecular weight of 4-5 kDa and a high rotational absorption rate. Therefore, the self-made functional peptide with a molecular weight of 4-5 kDa in this application exhibits a high transport absorption rate and strong activity.

[0021] Secondly, encapsulating functional peptides with liposomes enhances their local permeability to the skin. This allows highly active functional peptides with a molecular weight of 4-5 kDa to directly penetrate the stratum corneum and reach the basal layer, activating the synthesis of collagen and elastin, repairing the skin, and promoting skin rejuvenation. Simultaneously, it can also eliminate excess free radicals in the skin and inhibit the formation of membrane lipids, thus helping to delay skin aging.

[0022] Furthermore, by using hydrogenated lecithin and the self-made functional polypeptide liposomes of this application for compounding, the functional polypeptides with a molecular weight of 4-5kDa are promoted to penetrate evenly into the skin and be evenly distributed in the basal layer of the skin. This further improves the transport and absorption rate and activity of the functional polypeptides, which is beneficial to improving the ability of the functional polypeptides to repair the skin and delay skin aging.

[0023] Preferably, in step S3 of preparing the functional polypeptide liposomes, the molecular weight of the functional polypeptide is 4.25-4.75 kDa.

[0024] By adopting the above technical solution, functional polypeptide liposomes prepared using functional titanium plating with a molecular weight of 4.25-4.75kDa have higher transport and absorption rates and activity, and stronger ability to penetrate into the basal layer of the skin. They can further activate the synthesis of collagen and elastin in the skin, repair the skin, and promote skin rejuvenation.

[0025] Preferably, in step S1 of preparing the functional polypeptide liposomes, whey protein powder and bovine colostrum powder are mixed at a weight ratio of 1:(0.5-0.8).

[0026] By adopting the above technical solution and optimizing the ratio of whey protein powder and bovine colostrum powder, the enzymatic hydrolysis efficiency of the mixed protein solution by trypsin can be improved. Simultaneously, it also helps to increase the transport and absorption rate and activity of the functional peptides obtained from enzymatic hydrolysis, further enhancing their skin repair capabilities.

[0027] Preferably, in step S1 of preparing the functional polypeptide liposomes, the ultrasonic frequency during ultrasonic treatment is 35-55KHz and the ultrasonic time is 15-30min.

[0028] By adopting the above technical solution and optimizing the ultrasonic frequency and ultrasonic time of the mixed protein aqueous solution, the degree of protein denaturation in whey protein powder and bovine colostrum powder can be controlled, which is beneficial to improving the enzymatic hydrolysis effect of the mixed protein aqueous solution and enhancing the activity of functional peptides, thereby improving the skin's repair ability.

[0029] Preferably, in step S2 of the preparation of the functional polypeptide liposomes, the mass-volume concentration of the mixed protein aqueous solution after ultrasonic treatment is (5-8) g / 100 ml.

[0030] By adopting the above technical solution, the mass-volume concentration of the mixed protein aqueous solution after ultrasonic treatment is optimized, which not only improves the enzymatic hydrolysis efficiency of trypsin, but also enhances the activity and transport rate of functional peptides.

[0031] Preferably, in step S2 of the preparation of the functional polypeptide liposomes, the amount of trypsin added is 3500-4500 U / g.

[0032] By adopting the above technical solution and optimizing the amount of trypsin added, it is beneficial to improve the enzymatic hydrolysis efficiency of trypsin on the ultrasonically treated mixed protein aqueous solution.

[0033] Preferably, in step S2 of the preparation of the functional polypeptide liposomes, the pH value of the ultrasonically treated mixed protein aqueous solution obtained in S1 is 7-8, and the temperature is 50-65℃.

[0034] By adopting the above technical solution and optimizing the enzymatic hydrolysis conditions, it is beneficial to improve the enzymatic hydrolysis efficiency of trypsin on the ultrasonically treated mixed protein aqueous solution.

[0035] Preferably, the palmitoyl oligopeptide is composed of a mixture of palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7.

[0036] By adopting the above technical solution, palmitoyl oligopeptide composed of palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 can stimulate collagen synthesis, making the skin firmer and reducing skin sagging and uneven skin tone.

[0037] Secondly, this application provides a method for preparing a skin repair essence, using the following technical solution:

[0038] A method for preparing a skin repair essence includes the following steps:

[0039] Preparation of A1 essence powder: Functional polypeptide liposomes were mixed with hydrogenated lecithin, mannitol, yeast extract, palmitoyl oligopeptide and water, filtered and freeze-dried to obtain essence powder;

[0040] Preparation of A2 serum: The raw materials for the serum are stirred and mixed to obtain the serum;

[0041] Mixing A3 essence powder and essence: Mixing the essence powder and essence will produce a skin repair essence.

[0042] By adopting the above technical solution, the preparation method of the skin repair essence of this application is simple and convenient. The resulting skin repair essence has good skin repair function and is conducive to large-scale production.

[0043] In summary, this application has the following beneficial effects:

[0044] Because this application pre-treats the mixed protein aqueous solution with ultrasound and then uses trypsin for enzymatic hydrolysis, it retains functional peptides with a molecular weight cutoff of 4-5 kDa, which have a high transport and absorption rate and strong activity. The functional peptide liposomes made from the functional peptides can directly penetrate the stratum corneum of the skin and reach the basal layer of the skin, activating the synthesis of collagen and elastin in the skin, repairing the skin, promoting skin rejuvenation, and also clearing excess free radicals in the skin, inhibiting the formation of membrane lipids, and delaying skin aging. Detailed Implementation

[0045] The present application will be further described in detail below with reference to the embodiments.

[0046] The raw materials used in the embodiments and comparative examples of this application are all cosmetic grade.

[0047] The skin repair essences obtained in the embodiments and comparative examples of this application were tested for skin moisture content, skin elasticity, and acne scar repair. The testing methods are as follows:

[0048] Skin moisture and skin elasticity testing: Several healthy female volunteers aged 25-50 without skin diseases were randomly selected and randomly divided into groups of 5. Before the test, the volunteers used an intelligent skin analyzer, model MLB-D0028, manufactured by Guangzhou Meilaibao Beauty Equipment Co., Ltd., to test the skin moisture content and elasticity of their faces, which were recorded as the initial skin moisture content and initial skin elasticity.

[0049] Then, the volunteers applied the skin repair essence obtained from the embodiments and comparative examples of this application to their entire face. The amount used each time was 1mL. The method of use was as follows: 2ml of essence was dropped into 15mg of essence powder, and after dissolving, the skin repair essence was obtained.

[0050] Thirty minutes later, the skin moisture content and skin elasticity of the volunteers' foreheads were measured. The increase rate of skin moisture content and skin elasticity of each volunteer's forehead were calculated, and the average increase rate of skin moisture content and average increase rate of skin elasticity of each group of volunteers were also calculated.

[0051] Acne Scar Repair Test: Several female volunteers aged 25-50 with newly formed acne scars (within one week) were selected and randomly divided into groups of 5. Each time, 1 ml of skin repair essence was applied evenly to the area requiring repair, massaged for 3-5 minutes, and applied twice daily (morning and evening) for 28 days. The condition of the acne scars was observed and scored. The average score was used. The scoring criteria are as follows:

[0052] Acne scars with no obvious change: 0-3 points; acne scars with slight fading: 4-7 points; acne scars with significant fading: 8-10 points.

[0053] Preparation Example

[0054] Preparation Example 1

[0055] A functional polypeptide liposome is prepared by the following steps:

[0056] S1: Mix 500g whey protein powder, 200g bovine colostrum powder and 14000ml water to obtain a mixed protein aqueous solution with a mass-volume concentration of 5g / 100ml. Adjust the pH of the mixed protein aqueous solution to 5, the temperature to 20℃, and sonicate it at a frequency of 30KHz for 15min to obtain a sonicated mixed protein aqueous solution.

[0057] In the preparation example of this application, the whey protein powder is food grade and was purchased from Laizhen Jinfuyuan Biotechnology Co., Ltd.

[0058] Bovine colostrum powder, whose main components are immunoglobulins, lactoferrin, growth factors and proline-rich polypeptides, has a crude protein content of 64% and a moisture content of 2.17%. It is extracted from bovine milk.

[0059] In the mixed protein aqueous solution, the weight ratio of whey protein powder to bovine colostrum powder is 1:0.4.

[0060] S2: Adjust the pH of the ultrasonically treated mixed protein aqueous solution obtained in S1 to 7 and the temperature to 70℃, then add 3200U / g trypsin for enzymatic hydrolysis for 2.5h to obtain the enzymatic hydrolysis product;

[0061] Trypsin, enzyme activity 4000, enzyme activity retention rate 99%, arsenic content 0.00001, product number YS03214.

[0062] S3: Centrifuge the enzymatic hydrolysis product obtained in S2 at 4000 r / min for 10 min and collect the supernatant; boil the supernatant for 10 min, then dialyze, purify, concentrate, freeze dry, and obtain the functional peptide.

[0063] According to the test results, the molecular weight of the functional polypeptide obtained by dialysis in this embodiment is 4 kDa.

[0064] S4:

[0065] S41: First, hydrogenated soybean lecithin, cholesterol and vitamin E were dissolved in anhydrous ethanol at 60℃ to obtain a mixture with a mass-volume concentration of 20 mg / mL; the mixture was first sheared at 18000 r / min for 5 min, then homogenized at 15 MPa for 5 min, and then evaporated under reduced pressure in a water bath at 80℃ to obtain a liposome film.

[0066] The weight ratio of the above-mentioned hydrogenated soybean lecithin, cholesterol, and vitamin E is 10:1:0.1.

[0067] Add 0.5% Tween-80 aqueous solution to the liposome membrane, hydrate at 50℃ for 1 h, then shear at 18000 r / min for 5 min, and finally purify with Sephadex G-75 and break down the membrane with 1% Triton-100 to obtain liposomes.

[0068] S42: Dissolve liposomes in ethanol to obtain a liposome ethanol solution with a mass-volume concentration of 20 mg / mL; dissolve the functional polypeptide obtained in S3 in water to obtain a functional polypeptide aqueous solution with a mass-volume concentration of 20 mg / mL; add the liposome ethanol solution to the functional polypeptide aqueous solution, sonicate and stir at a frequency of 25 kHz for 1 min, filter, concentrate to remove ethanol, and then dry to obtain functional polypeptide liposomes.

[0069] Preparation Examples 2-5

[0070] A functional polypeptide liposome differs from preparation example 1 in that, in step S3 of the preparation of the functional polypeptide liposome, the molecular weight of the functional polypeptide obtained by dialysis retention is shown in Table 1.

[0071] Table 1 shows the molecular weight (kDa) of the functional peptides in Preparation Examples 2-5.

[0072]

[0073] Preparation Examples 6-8

[0074] A functional polypeptide liposome differs from preparation example 3 in that, in step S1 of the preparation of the functional polypeptide liposome, the weight ratio of whey protein powder and bovine colostrum powder in the mixed protein aqueous solution is different. The weights of whey protein powder and bovine colostrum powder and the volume of water in the mixed protein aqueous solution are shown in Table 2.

[0075] Table 2 shows the weight (g) of whey protein powder and bovine colostrum powder, and the volume (mL) of water in Preparation Examples 2-4.

[0076]

[0077] Preparation Examples 9-10

[0078] A functional polypeptide liposome differs from preparation example 7 in that the mass-volume concentration of the mixed protein aqueous solution is different in step S1 of the preparation of the functional polypeptide liposome. The mass-volume concentration of the mixed protein aqueous solution is shown in Table 3.

[0079] Table 3 shows the mass-volume concentration (g / mL) of the mixed protein aqueous solution in Preparation Examples 9-10.

[0080]

[0081] Preparation Examples 11-13

[0082] A functional polypeptide liposome differs from preparation example 9 in that the ultrasonic frequency and time of the ultrasonic treatment in step S1 of the preparation step of the functional polypeptide liposome are different. The ultrasonic frequency and time of the ultrasonic treatment are shown in Table 4.

[0083] Table 4 shows the ultrasonic frequency (kHz) and time (min) of the ultrasonic treatment in Examples 11-13.

[0084]

[0085] Preparation Examples 14-17

[0086] A functional polypeptide liposome differs from preparation example 12 in that the amount of trypsin added in step S2 of the preparation of the functional polypeptide liposome is different, as shown in Table 5.

[0087] Table 5 shows the amount of trypsin added (U / g) in Preparation Examples 14-17.

[0088]

[0089] Preparation Example 18

[0090] A functional polypeptide liposome differs from preparation example 15 in that the pH value and temperature of the ultrasonically treated mixed protein aqueous solution obtained in step S2 of the preparation step S1 are different, as shown in Table 5.

[0091] Table 6 shows the pH value and temperature (°C) in Preparation Example 18.

[0092]

[0093] Example

[0094] Example 1

[0095] A skin repair essence, comprising essence powder and essence liquid;

[0096] The components and their corresponding weights in the above-mentioned essence powder and essence are shown in Table 5, and are prepared by the following steps: Preparation of A1 essence powder:

[0097] Mannitol, functional polypeptide liposomes, hydrogenated lecithin, palmitoyl oligopeptide, yeast extract and water were mixed and stirred at 800 r / min for 10 min, filtered and freeze-dried to obtain the essence powder.

[0098] The functional polypeptide liposomes used in this application are those prepared in Preparation Example 1.

[0099] Preparation of A2 Essence:

[0100] A21: Mix disodium EDTA, 1,3-butanediol, propylene glycol, glycerol, sodium PCA, sodium hyaluronate, betaine, and water (61.78% of the total weight of water), stir and mix at 85°C and 800 r / min for 10 min, and keep warm for 30 min to obtain mixture A.

[0101] A22: Mix p-hydroxyacetophenone, 1,2-hexanediol and butanediol, and stir for 20 min at 60°C and 800 r / min to obtain mixture B; add mixture B to mixture A at 60°C, and stir for 20 min at 800 r / min to obtain mixture C.

[0102] A23: Mix the remaining water, Centella asiatica extract, Polygonum cuspidatum root extract, Scutellaria baicalensis extract, tea extract, Glycyrrhiza glabra root extract, Chamomile extract, Rosmarinus officinalis leaf extract, Portulaca oleracea extract, L-carnosine, dipotassium glycyrrhizate, nonapeptide-1, and niacinamide, and stir for 20 minutes at 50°C and 800 rpm to obtain mixture D; add mixture D to mixture C at 45°C and stir for 20 minutes to obtain the essence.

[0103] Mixing A3 essence powder and essence: After mixing the essence powder obtained from A1 and the essence obtained from A23, a skin repair essence is obtained;

[0104] In the above skin repair essence, the essence powder and essence liquid are mixed at a ratio of 15mg essence powder to 2ml essence liquid.

[0105] Example 2-3

[0106] A skin repair essence differs from Example 1 in that the components of the essence powder and their corresponding weights are shown in Table 5.

[0107] Table 5. Components and their weights (kg) in the essence powder

[0108]

[0109]

[0110] Three groups of volunteers were selected to test the skin moisture content and skin elasticity of the skin repair essence obtained in Examples 1-3 above. Three groups of volunteers were also selected to test the skin acne scar repair. The test results are shown in Table 6.

[0111] Table 6 shows the performance test results for Examples 1-3.

[0112]

[0113] As shown in Table 6, the skin repair essences obtained in Examples 1-3 of this application, after 30 minutes of use by volunteers, resulted in an average increase in skin moisture content of 43.5-44.1%, an average increase in skin elasticity of 23-23.5%, and a skin acne scar repair score of 7. This indicates that the skin repair essences obtained in Examples 1-3 of this application have excellent skin repair capabilities, helping to improve skin moisture, enhance skin elasticity, and delay skin aging.

[0114] Examples 4-6

[0115] A skin repair essence differs from Example 2 in that the molecular weight of the functional polypeptides obtained by dialysis retention in step S3 of the preparation step of the lipid functional polypeptide liposomes in the essence powder is different.

[0116] The functional polypeptide liposomes in this application are prepared using the preparation examples shown in Table 7.

[0117] Table 7 shows the functional polypeptide liposomes used in Examples 4-7, prepared from the examples listed in the table below.

[0118] Example Example 2 Example 4 Example 5 Example 6 Example 7 Preparation Example Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5

[0119] Four groups of volunteers were selected to test the skin moisture content and skin elasticity of the skin repair essence obtained in Examples 4-7 above. Four groups of volunteers were also selected to test the skin acne scar repair. The test results are shown in Table 8.

[0120] Table 8 shows the performance test results for Examples 4-7.

[0121]

[0122] As shown in Table 8, the skin repair essences obtained in Examples 4-6 of this application, after 30 minutes of use by volunteers, resulted in an average increase in skin hydration of 46.5-47.5%, an average increase in skin elasticity of 25.4-25.7%, and a skin acne scar repair score of 8 points. These figures are significantly higher than those of volunteers using the skin repair essences obtained in Examples 2 and 7. This indicates that the functional peptides in the essence powder, with a molecular weight of 4.25-4.75 kDa, can better enhance the skin's repair ability and delay skin aging.

[0123] Examples 8-10

[0124] A skin repair essence differs from Example 5 in that the weight ratio of whey protein powder and bovine colostrum powder in step S1 of the preparation step of the functional polypeptide liposomes is different. The functional polypeptide liposomes in this embodiment are those obtained from the preparation examples shown in Table 9.

[0125] Table 9 shows the functional polypeptide liposomes used in Examples 8-10, prepared according to the preparation examples in the table below.

[0126] Example Example 5 Example 8 Example 9 Example 10 Preparation Example Preparation Example 3 Preparation Example 6 Preparation Example 7 Preparation Example 8

[0127] Three groups of volunteers were selected to test the skin moisture content and skin elasticity of the skin repair essence obtained in Examples 8-10 above. Three groups of volunteers were also selected to test the skin acne scar repair. The test results are shown in Table 10.

[0128] Table 10 shows the performance test results for Examples 8-10.

[0129]

[0130]

[0131] As shown in Table 10, the skin repair essences obtained in Examples 8-10 of this application, after 30 minutes of use by volunteers, resulted in an average increase in skin hydration of 48.0-48.2%, an average increase in skin elasticity of 26.1-26.3%, and a skin acne scar repair score of 9 points. These figures are significantly higher than those of volunteers using the skin repair essence obtained in Example 5. This indicates that in the total raw materials of the skin repair essence, in the essence powder, in step S1 of preparing functional polypeptide liposomes, whey protein powder and bovine colostrum powder are mixed at a weight ratio of 1:(0.5-0.8). The resulting functional polypeptide liposomes have a higher transport and absorption rate and stronger activity, which can better improve the skin's repair ability and delay skin aging.

[0132] Examples 11-12

[0133] A skin repair essence differs from Example 9 in that the mass-volume concentration of the mixed protein aqueous solution in step S1 of the preparation step of the functional polypeptide liposomes in the essence powder is different. The functional polypeptide liposomes in this application embodiment are those prepared using the preparation examples shown in Table 11.

[0134] Table 11 shows the functional polypeptide liposomes used in Examples 11-12, prepared from the preparation examples listed below.

[0135] Example Example 9 Example 11 Example 12 Preparation Example Preparation Example 7 Preparation Example 9 Preparation Example 10

[0136] Two groups of volunteers were selected to test the skin moisture content and skin elasticity of the skin repair essence obtained in Examples 11-12 above. Two groups of volunteers were also selected to test the skin acne scar repair. The test results are shown in Table 12.

[0137] Table 12 shows the performance test results for Examples 11-12.

[0138]

[0139] As shown in Table 12, the skin repair essences obtained in Examples 9 and 11-12 of this application, after 30 minutes of use by volunteers, showed an average increase in skin moisture content of 48.2-48.9%, an average increase in skin elasticity of 26.3-26.8%, and a skin acne scar repair score of 9 points. This indicates that, in the total raw materials of the skin repair essence, the concentration of the mixed protein aqueous solution after ultrasonic treatment in step S2 of the preparation of functional polypeptide liposomes in the essence powder is (5-8) g / 100 ml, which can further enhance the skin's repair ability and delay skin aging.

[0140] Examples 13-15

[0141] A skin repair essence differs from Example 11 in that the ultrasonic frequency and duration of the ultrasonic treatment in step S1 of the preparation step of the functional polypeptide liposomes in the essence powder are different. The functional polypeptide liposomes in this application embodiment are those prepared using the preparation examples shown in Table 13.

[0142] Table 13 shows the functional polypeptide liposomes used in Examples 13-15, which were prepared from the preparation examples in the table below.

[0143] Example Example 11 Example 13 Example 14 Example 15 Preparation Example Preparation Example 9 Preparation Example 11 Preparation Example 12 Preparation Example 13

[0144] Three groups of volunteers were selected to test the skin moisture content and skin elasticity of the skin repair essence obtained in Examples 13-15 above. Three groups of volunteers were also selected to test the skin acne scar repair. The test results are shown in Table 14.

[0145] Table 14 shows the performance test results for Examples 13-15.

[0146]

[0147] As shown in Table 14, the skin repair essences obtained in Examples 13-15 of this application, after 30 minutes of use by volunteers, exhibited significantly higher average increases in skin hydration, skin elasticity, and acne scar repair scores compared to those obtained in Example 11. This indicates that in the preparation step S1 of the functional polypeptide liposomes, the ultrasonic frequency of 35-55 kHz and the ultrasonic time of 15-30 minutes in the ultrasonic treatment of the total raw materials of the skin repair essence enhance the activity of the enzymatic hydrolysates, thereby improving the skin's repair capabilities and delaying skin aging.

[0148] Examples 16-19

[0149] A skin repair essence differs from Example 14 in that the amount of trypsin added in step S2 of the preparation of functional polypeptide liposomes in the essence powder is different. The functional polypeptide liposomes in this application embodiment are those prepared as shown in Table 15.

[0150] The functional polypeptide liposomes used in Examples 9-11 were prepared from the preparation examples in the table below.

[0151]

[0152]

[0153] Four groups of volunteers were selected to test the skin hydration and skin elasticity of the skin repair essence obtained in Examples 16-19 above. The test results are shown in Table 14.

[0154] Table 15 shows the performance test results for Examples 16-19.

[0155]

[0156] As shown in Table 15, the skin repair essences obtained in Examples 16-17 of this application, after 30 minutes of use by volunteers, exhibited significantly higher average increases in skin hydration, skin elasticity, and acne scar repair scores compared to those obtained in Examples 14 and 19. This indicates that in the total raw materials of the skin repair essence, the addition of trypsin at 3500-4500 U / g in step S2 of the preparation of functional polypeptide liposomes enhances the activity of the enzymatic hydrolysate, thereby improving the skin's repair capabilities and delaying skin aging.

[0157] Example 20

[0158] A skin repair essence differs from Example 17 in that the pH value and temperature of the ultrasonically treated mixed protein aqueous solution obtained in step S1 of the preparation step S2 of the essence powder for the functional polypeptide liposomes are different.

[0159] The functional polypeptide liposomes used in this application are those prepared in Preparation Example 18.

[0160] Example 21

[0161] A skin repair essence, which differs from Example 17 in that the essence powder contains palmitoyl oligopeptide composed of a mixture of 3.2 kg palmitoyl tripeptide-1 and 2.4 kg palmitoyl tetrapeptide-7.

[0162] Two groups of volunteers were selected to test the skin hydration and skin elasticity of the skin repair essence obtained in Examples 20-21 above. The test results are shown in Table 16.

[0163] Table 16 shows the performance test results for Examples 20-21.

[0164]

[0165] As shown in Table 16, the skin repair essences obtained in Examples 20 and 21 of this application, after 30 minutes of use by volunteers, showed significantly higher average increases in skin hydration, skin elasticity, and acne scar repair scores compared to those obtained after using the skin repair essence in Example 17. This indicates that the palmitoyl oligopeptide in the total raw materials of the skin repair essence, composed of a mixture of palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, is beneficial in enhancing the skin's repair capabilities and delaying skin aging.

[0166] Comparative Example

[0167] Comparative Example 1

[0168] A skin repair essence differs from Example 1 in that the preparation step S1 of the functional polypeptide liposomes is different.

[0169] The preparation step S1 of functional polypeptide liposomes is as follows:

[0170] S1: Mix 500g whey protein powder, 200g bovine colostrum powder and 14000ml water to obtain a mixed protein aqueous solution with a mass-volume concentration of 5g / 100ml. Adjust the pH of the mixed protein aqueous solution to 5 and the temperature to 20℃. Then, stir the mixture at 5000r / min for 15min to obtain the stirred mixed protein aqueous solution.

[0171] Comparative Example 2

[0172] A skin repair essence differs from Example 1 in that, in step S3 of preparing the functional polypeptide liposomes, the molecular weight of the functional polypeptide obtained by dialysis retention is 3 kDa.

[0173] Comparative Example 3

[0174] A skin repair essence differs from Example 1 in that, in step S3 of preparing the functional polypeptide liposomes, the molecular weight of the functional polypeptide obtained by dialysis retention is 6 kDa.

[0175] Comparative Example 4

[0176] A skin repair essence differs from Example 1 in that the preparation steps of the functional polypeptide liposomes are different.

[0177] The preparation steps for functional polypeptide liposomes are as follows:

[0178] S1: Mix 500g whey protein powder, 200g bovine colostrum powder and 14000ml water to obtain a mixed protein aqueous solution with a mass-volume concentration of 5g / 100ml. Adjust the pH of the mixed protein aqueous solution to 5 and the temperature to 20℃. Then, stir the mixture at 5000r / min for 15min to obtain the stirred mixed protein aqueous solution.

[0179] In the mixed protein aqueous solution, the weight ratio of whey protein powder to bovine colostrum powder is 1:0.4.

[0180] S2: Adjust the pH of the mixed protein aqueous solution obtained after stirring in S1 to 7 and the temperature to 70℃, then add 3200U / g trypsin for enzymatic hydrolysis for 2.5h to obtain the enzymatic hydrolysis product;

[0181] Trypsin, enzyme activity 4000, enzyme activity retention rate 99%, arsenic content 0.00001, product number YS03214.

[0182] S3: Centrifuge the enzymatic hydrolysis product obtained in S2 at 4000 r / min for 10 min and collect the supernatant; boil the supernatant for 10 min, then dialyze, purify, concentrate, freeze dry, and obtain the functional peptide.

[0183] According to the test results, the molecular weight of the functional polypeptide obtained by dialysis in this embodiment is 3 kDa.

[0184] Comparative Example 5

[0185] A skin repair serum, which differs from Example 1 in that it uses an equal amount of cholesterol instead of hydrogenated lecithin.

[0186] Five groups of volunteers were selected to test the skin hydration and elasticity of the skin repair essence obtained from the above comparative examples 1-5. Five groups of volunteers were also selected to test the skin acne scar repair. The test results are shown in Table 6.

[0187] Table 17 shows the performance test results for Comparative Examples 1-5.

[0188]

[0189] Based on the skin repair essence obtained in Example 1 and Comparative Example 1, and referring to Table 17, it can be seen that after volunteers used the skin repair essence obtained in Example 1, compared with volunteers using the skin repair essence obtained in Comparative Example 1, the average increase rate of skin moisture content increased by 12.99%, the average increase rate of skin elasticity increased by 29.21%, and the skin acne scar repair score was significantly improved. This indicates that in the preparation step S1 of the functional polypeptide liposomes in the total raw materials for preparing the skin repair essence, the ultrasonic treatment of the mixed protein aqueous solution can improve the skin repair ability of the final skin repair essence, which is beneficial for increasing skin moisture, improving skin elasticity, and delaying skin aging.

[0190] Based on the skin repair essence obtained in Example 1 and the skin repair essence obtained in Comparative Examples 2-3, and referring to Table 17, it can be seen that after volunteers used the skin repair essence obtained in Example 1, compared with volunteers using the skin repair essence obtained in Comparative Examples 2-3, the average increase rate of skin moisture content increased by 18.53-21.51%, the average increase rate of skin elasticity increased by 37.72-41.98%, and the skin acne scar repair score was significantly improved. This indicates that in the total raw materials for preparing the skin repair essence, in step S3 of preparing functional polypeptide liposomes, the functional polypeptides obtained by dialysis retention have a molecular weight of 4-5 kDa, which can directly penetrate the stratum corneum of the skin, reach the basal layer of the skin, activate the synthesis of collagen and elastin, repair the skin, and promote skin rejuvenation.

[0191] Based on the skin repair essence obtained in Example 1 and Comparative Example 4, and referring to Table 17, it can be seen that after volunteers used the skin repair essence obtained in Example 1, compared with volunteers using the skin repair essence obtained in Comparative Example 4, the average increase rate of skin moisture content increased by 33.44%, the average increase rate of skin elasticity increased by 41.98%, and the skin acne scar repair score was significantly improved. This indicates that in the total raw materials for preparing the skin repair essence, the ultrasonic treatment of the mixed protein aqueous solution in step S1 of the preparation step S3 ...

[0192] Based on the skin repair essence obtained in Example 1 and Comparative Example 5, and referring to Table 17, it can be seen that after volunteers used the skin repair essence obtained in Example 1, compared with volunteers using the skin repair essence obtained in Comparative Example 5, the average increase rate of skin moisture content increased by 24.64%, the average increase rate of skin elasticity increased by 39.39%, and the skin acne scar repair score was significantly improved. This indicates that in the total raw materials for preparing the skin repair essence, hydrogenated lecithin can further promote the penetration ability of functional polypeptide liposomes, which is beneficial for skin repair and promotes skin rejuvenation.

[0193] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A skin repair essence, characterized in that, It consists of the following components by weight percentage: Mannitol 32-35%; Functional polypeptide liposomes 22-26%; Hydrogenated lecithin 14-18%; Palmitoyl oligopeptide 4.4-7.2%; Yeast extract 0.3-0.6%; Water is the remainder; The functional polypeptide liposomes include the following preparation steps: S1: Mix whey protein powder, bovine colostrum powder and water to obtain a mixed protein aqueous solution. Sonicate the mixed protein aqueous solution to obtain a sonicated mixed protein aqueous solution. S2: Adjust the pH and temperature of the ultrasonically treated mixed protein aqueous solution obtained in S1, and then add trypsin for enzymatic hydrolysis to obtain the enzymatic hydrolysis product; S3: Centrifuge the enzymatic hydrolysis product obtained in S2 and collect the supernatant; boil the supernatant, dialyze, purify, filter, freeze dry, and the functional peptide is obtained. S4: After mixing the liposomes and the functional peptides obtained in S3, the mixture is filtered and dried to obtain functional peptide liposomes. In S3, the molecular weight of the functional peptide is 4-5 kDa; In step S1 of the preparation of the functional polypeptide liposomes, the ultrasonic frequency in the ultrasonic treatment is 35-55KHz and the ultrasonic time is 15-30min. In step S2 of the preparation of the functional polypeptide liposomes, the pH value of the ultrasonically treated mixed protein aqueous solution obtained in S1 is 7-8, and the temperature is 50-65℃. In step S3 of the preparation of the functional polypeptide liposomes, the molecular weight of the functional polypeptide is 4.25-4.75 kDa. The palmitoyl oligopeptide is composed of a mixture of palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7; In step S1 of the preparation of the functional polypeptide liposomes, whey protein powder and bovine colostrum powder are mixed at a weight ratio of 1:(0.5-0.8). In step S2 of the preparation of the functional polypeptide liposomes, the mass-volume concentration of the mixed protein aqueous solution after ultrasonic treatment is (5-8) g / 100ml; In step S2 of the preparation of the functional polypeptide liposomes, the amount of trypsin added is 3500-4500 U / g.

2. The method for preparing the skin repair essence according to claim 1, characterized in that, Includes the following steps: Preparation of A1 essence powder: Functional polypeptide liposomes were mixed with hydrogenated lecithin, mannitol, yeast extract, palmitoyl oligopeptide and water, filtered and freeze-dried to obtain essence powder; Preparation of A2 serum: The raw materials for the serum are stirred and mixed to obtain the serum; Mixing A3 essence powder and essence: Mixing the essence powder and essence will produce a skin repair essence.

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