A fumigation type air sterilizing agent using a compound of essential oil of plant extraction, and a preparation method and application thereof

Abstract

The application discloses a fumigation type air sterilizing agent adopting a plant-extracted essential oil compound and a preparation method and application thereof, which is composed of the following components in parts by weight: a sterilization synergist 6-20 parts, a plant-extracted essential oil compound 20-80 parts, an alcohol solvent 1-50 parts, an essential oil modification blend 0.1-3 parts and an antioxidant 0.1-1.5 parts. The sterilization synergist and the plant-extracted essential oil compound are compounded in a specific ratio to prepare the fumigation type air sterilizing agent, the sterilization rate of staphylococcus gas aerosol in the air can reach more than 99% through heating and volatilization, and the natural bacteria extinction rate can reach more than 95%, so that the fumigation type air sterilizing agent can be used for preparing an electric heating sheet, an electric heating incense, a joss stick, a sanitary incense, a disc type incense, a bamboo branch incense or a microwave spray and the like.

Description

Technical Field

[0001] This invention relates to a fumigation-type air disinfectant formulated with plant-derived essential oils, its preparation method, and its application. Specifically, it relates to a volatile bactericide formulated with plant-derived essential oils for enhanced efficacy, its preparation method, and its application, belonging to the field of daily chemical products technology. Background Technology

[0002] Air contains a large number of microorganisms and bacteria that are harmful to human health, such as Staphylococcus aureus. Many common air disinfection methods exist, including opening windows for ventilation, ultraviolet disinfection, mopping or spraying with disinfectants, and air sterilization. Air disinfectants are commonly used liquid preparations that can kill bacteria and purify the air, but they are mostly synthetic substances. While disinfecting, sterilizing, and freshening the air, they also pose certain risks to human health, and their odor is not ideal. In recent years, many natural plant essential oils and some traditional Chinese medicine extracts have been found to have antibacterial, bactericidal, and air-freshening functions, and are safe and non-toxic. However, the bactericidal effect of these natural plant extracts is significantly lower than that of synthetic substances. Therefore, in the development and research of air disinfectants, it is of great significance to find ways to incorporate these natural plant extracts as effective substances in air disinfectants, while also improving the antibacterial and bactericidal properties of the plants and ensuring safety for human health.

[0003] Patent CN1592578A discloses a microbial preparation containing essential oils or their derivatives. By mixing the essential oil components or their derivatives, or various mixtures thereof, with other preparations such as ethanol, emulsifiers, capsules, and biocides, it can prevent the growth of Staphylococcus aureus and Candida albicans, thus protecting fruits and vegetables from post-harvest decay and preventing meat or fish spoilage. However, the patent only examines the preservative effect of this preparation after soaking fruits or adding it to broth; whether the preparation can volatilize and kill bacteria in the air remains uncertain.

[0004] The invention patent with publication number CN114259430A discloses a plant essential oil antibacterial spray and its preparation method. Designed for antibacterial disinfection of human skin and mucous membranes, it uses a compound of spearmint essential oil, lavender essential oil, Atractylodes lancea essential oil, Artemisia argyi essential oil, and Artemisia annua essential oil as effective antibacterial ingredients. The components of these plant essential oils include organic compounds such as alcohols, aldehydes, acids, phenols, esters, ketones, and terpenes. Through the compound use of these plant essential oils, a synergistic effect can be achieved, enhancing the bactericidal activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, etc. When applied to skin and mucous membrane hygiene, it achieves moisturizing, soothing, and anti-inflammatory effects.

[0005] The invention patent with publication number CN115943970A discloses a mosquito repellent composition with bactericidal and deodorizing effects. By using mosquito repellent components and bactericidal and deodorizing components in combination, especially the combination of compound mosquito repellent essential oil and Lonza CS1000 deodorizing and bactericidal agent, a synergistic effect can be produced, thereby exerting better bactericidal and deodorizing effects. It is very suitable for mosquito repellency in the environment or on the skin. However, its bactericidal active substance is Lonza CS1000 deodorizing and bactericidal agent, and the bactericidal effect test is carried out by immersion quantitative bactericidal test method. It can only be used to prove that the mosquito repellent composition has certain bactericidal and bacteriostatic properties after contact with the human skin surface. Whether the mosquito repellent composition can be used for volatilization and bactericidal in the air still needs to be verified by experimental results.

[0006] It is evident that existing technologies can enhance the antibacterial effect of natural plant-based bactericides to some extent through various methods. However, the bactericides currently disclosed in patents are all contact bactericides, i.e., bactericides for the skin surface or object surface, and there are no specific bactericides for airborne microorganisms such as Staphylococcus aureus.

[0007] In a report on air disinfection using plant essential oil fumigation, Hu Yongcheng et al. studied the air disinfection effect and safety of a traditional Chinese medicine air disinfectant in their study, "Air Sterilization Effect and Toxicity Study of a Traditional Chinese Medicine Air Disinfectant." The main effective components of this traditional Chinese medicine air disinfectant are extracts of various traditional Chinese medicines, including eucalyptus oil and clove oil. The disinfectant underwent air disinfection effect identification tests and acute oral toxicity (LD50) tests. 50 ), a complete skin irritation test, an eye irritation test, and an acute inhalation toxicity test (LC). 50 Micronucleus tests and other assays showed that this traditional Chinese medicine air disinfectant had a good bactericidal effect against Staphylococcus aureus, low toxicity, and was relatively safe to use, making it suitable for freshening and disinfecting general household spaces. However, in its air disinfection efficacy evaluation test, a dosage of 2 ml / m³ was used. 3 Spray disinfection, one room with an area of ​​15m² 2 Based on a floor height of 2m, the room volume is 30m². 3 The amount of pesticide sprayed is 60ml. At this concentration, people must leave the room, and the sprayed solution cannot fully contact the bacteria or viruses in the air, so it quickly settles to the ground, resulting in a lot of waste.

[0008] In their study, "Research and Efficacy Evaluation of Plant Essential Oil-Based Air Sterilizers and Fresheners," Wu Huiqing et al. evaluated the air sterilization effects and safety of plant essential oils such as cedarwood, cinnamon, lemongrass, eucalyptus, geranium, peppermint, thyme, tea tree, coriander, and lemongrass. Using 0.1 ml of liquid compound essential oil per cubic meter of space for heating and fumigation for 0.5 hours, the sterilization rate reached 99.93%. Preliminary experiments showed that the disinfectant had low irritation and good safety. However, in their quantitative evaluation experiment of air sterilization effect, only a 1m³ of space was used... 3The air chamber was used for testing, and the disinfection method recommended by the technical specifications is not practical.

[0009] It is evident that while the plant-extracted essential oils described in existing reports can meet the air disinfection effect test under specific conditions, these test conditions do not meet the real-world usage environment and are therefore impractical. Thus, how to improve the volatilization and sterilization of plant essential oils during normal use is precisely the problem that this invention aims to solve. Summary of the Invention

[0010] This invention aims to address the problem that existing plant essential oil disinfectants fail to achieve ideal sterilization effects after fumigation and volatilization. It provides a fumigation-type air disinfectant formulated with a blend of plant essential oils. This mixture is prepared using a specific ratio of bactericidal synergists and plant essential oils, and upon heating and volatilization, achieves a sterilization rate of over 99% for Staphylococcus aureus aerosols in the air and an elimination rate of over 95% for naturally occurring bacteria. Furthermore, this invention also provides a method for preparing this fumigation-type air disinfectant and its application in fumigation-type disinfection products.

[0011] This invention is achieved through the following technical solution: a fumigation-type air disinfectant formulated with plant-derived essential oils, comprising the following components in parts by weight: 6-20 parts of a bactericidal synergist, 20-80 parts of a plant-derived essential oil compound, 1-50 parts of an alcohol solvent, 0.1-3 parts of an essential oil modifier, and 0.1-1.5 parts of an antioxidant.

[0012] The bactericidal synergist is ethyl 3-(N-butyl-acetamido)propionate, DEET, insect repellent 3535, or imipenem; the plant extract essential oil compound composition is selected from at least two of peppermint essential oil, perilla essential oil, catnip essential oil, artemisia essential oil, clove essential oil, thyme essential oil, tea tree essential oil, cinnamon essential oil, and oregano essential oil.

[0013] Furthermore, the fumigation-type air disinfectant is composed of the following components in parts by weight: 6-15 parts of ethyl 3-(N-butyl-acetamido)propionate, 20-50 parts of plant essential oil compound composition, 10-30 parts of alcohol solvent, 0.1-1.1 parts of essential oil modifier, and 0.1-1.0 parts of antioxidant.

[0014] In the fumigation-type air disinfectant, the weight percentage of the plant extract essential oil compound composition accounts for more than 20% of the total weight of the fumigation-type air disinfectant; the weight percentage of the plant extract essential oil compound composition and the bactericidal synergist accounts for more than 40% of the total weight of the fumigation-type air disinfectant; and the weight ratio of the bactericidal synergist to the plant extract essential oil compound composition is 1:(2-3).

[0015] The plant-based essential oil compound composition comprises a combination of the following components:

[0016] (1) Thyme essential oil and oregano essential oil, by weight, thyme essential oil : oregano essential oil = 1 : 1;

[0017] (2) Perilla essential oil and clove essential oil, by weight, perilla essential oil : clove essential oil = 1 : 1;

[0018] (3) Peppermint essential oil, catnip essential oil and mugwort essential oil, by weight parts, peppermint essential oil: catnip essential oil: mugwort essential oil = 1:2:2;

[0019] (4) Clove essential oil, thyme essential oil, tea tree essential oil and cinnamon essential oil, in parts by weight, clove essential oil: thyme essential oil: tea tree essential oil: cinnamon essential oil = 1:1:1:1.

[0020] The plant-derived essential oil compound composition contains the following components in parts by weight: 1-3 parts peppermint essential oil, 1-8 parts perilla essential oil, 1-5 parts catnip essential oil, 2-10 parts mugwort essential oil, 1-5 parts clove essential oil, 1-5 parts thyme essential oil, 1-10 parts tea tree essential oil, 1-8 parts cinnamon essential oil, and 2-8 parts oregano essential oil.

[0021] The alcohol solvent is selected from at least one of ethanol, phenoxyethanol, and n-propanol.

[0022] The alcohol solvent contains the following components in parts by weight: 1-30 parts ethanol, 1-20 parts phenoxyethanol, and 1-18 parts n-propanol.

[0023] The essential oil modifier is selected from at least one of ethyl 2,4-decadienoate, methyl dihydrojasmonic acid, and dihydromyrcenol.

[0024] The essential oil modifier blend comprises the following components in parts by weight: 1-2 parts ethyl 2,4-decadienoate, 2-4 parts methyl dihydrojasmonic acid, and 2-4 parts dihydromyrcenol.

[0025] A method for preparing a fumigation-type air disinfectant using plant-derived essential oils, wherein the components of the fumigation-type air disinfectant are mixed according to the above-mentioned component ratio.

[0026] An application of a fumigation-type air sterilizer formulated with plant-derived essential oils, wherein the fumigation-type air sterilizer is used as an additive in the preparation of electric heating elements, electric incense, incense sticks, sanitary incense, coil incense, bamboo incense, or microwave spray.

[0027] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0028] (1) The ingredients of this invention are safe to use, environmentally friendly, and free from irritation and corrosiveness, wherein:

[0029] The bactericidal synergist's bactericidal raw material comes from the known mosquito repellent component ethyl 3-(N-butyl-acetamido)propionate, a broad-spectrum and highly effective insect repellent. Its chemical structure is a naturally derived derivative of β-alanine, belonging to the ester solvent class. It has an acute oral LD50 value in SD rats. 50 >5000 mg / kg bw, classified as practically non-toxic; acute percutaneous LD50 in SD rats 50 >5000 mg / kg bw, classified as practically non-toxic; acute inhalation LC50 in SD rats 50 2h > 10000 mg / m 3 It is practically non-toxic; a single complete skin irritation test on rabbits showed no irritation. It caused slight eye irritation in rabbits; the mouse bone marrow polychromatic erythrocyte micronucleus test was negative. Therefore, the source and use of this bactericidal synergist are safe and without toxic side effects.

[0030] The plant-based essential oil blend is derived from peppermint oil, perilla oil, catnip oil, mugwort oil, clove oil, thyme oil, tea tree oil, cinnamon oil, and oregano oil. These are all commonly used daily necessities, derived from plant extracts, biodegradable, and environmentally friendly.

[0031] Alcohol solvents such as ethanol, phenoxyethanol, and n-propanol are commonly used in cosmetics and are safe for human use.

[0032] Essential oils are used as fragrance raw materials in daily cosmetics and are widely used in cosmetics and food. They are safe for human consumption.

[0033] Antioxidants are widely used in food and cosmetics and are safe for humans.

[0034] Therefore, it can be seen that the present invention uses components that are safe for humans and have no toxic side effects to prepare fumigation air disinfectant. It does not contain chemical reagents such as oxidants and chlorides, and does not have the disadvantages of traditional disinfectants such as irritation and corrosiveness, thus meeting the requirements for safe use of air disinfectant.

[0035] (2) This invention introduces insect repellent as a bactericidal synergist in plant essential oil bactericides for the first time. By compounding in a specific ratio, it serves as an effective bactericidal substance in fumigation-type air disinfectant, which has a strong bactericidal effect. In particular, after heating and volatilization, it can kill Staphylococcus aureus aerosol and natural bacteria in the air, and has a relatively long-lasting bactericidal effect.

[0036] (3) The alcohol solvent used in this invention can harmonize the strong pungent smell of natural plant essential oils and react with the acid and aldehyde components in the compound composition of plant essential oils to form ester compounds, making them pleasantly fragrant.

[0037] (4) The essential oil modifier used in this invention can control the evaporation rate of essential oils and further modulate the stimulating flavor of essential oils.

[0038] (5) The antioxidant used in this invention can alleviate the oxidation of unsaturated structures such as olefins, aldehydes and ketones in essential oils, so that the product does not change color and the components remain stable.

[0039] In summary, this invention provides an air disinfectant with enhanced sterilization effect through heating and volatilization. It uses a specific amount of bactericidal synergist and a compound of plant-derived essential oils as the effective bactericidal substances, combined with alcohol solvents, essential oil modifiers, and antioxidants. This enhances the bactericidal effect of the volatile components, achieving a kill rate of over 99% against Staphylococcus aureus aerosols in the air and an elimination rate of over 95% against naturally occurring bacteria. Furthermore, the ingredients are from safe sources and have no irritating or toxic side effects on the human body. Attached Figure Description

[0040] Figure 1 This is a schematic diagram illustrating an application scenario of the present invention. Detailed Implementation

[0041] The invention's objective, technical solution, and beneficial effects will be further explained in detail below.

[0042] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the claimed invention. Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0043] We know that using chemical disinfectants poses numerous risks, including: ingestion can cause a burning sensation in the mouth, pharynx, esophagus, and stomach, along with nausea and vomiting; inhalation of large amounts can cause significant respiratory irritation symptoms such as coughing, shortness of breath, and difficulty breathing; splashing into the eyes can burn the cornea and conjunctiva; and skin contact with high concentrations of disinfectants may cause localized blisters. To avoid these risks, plant essential oils can be used as an alternative to chemical disinfectants. It is known that plant essential oils release various fragrances during fumigation, which have disease-preventing and insect-repelling effects. Furthermore, the ancient custom of burning mugwort during the Dragon Boat Festival not only kills bacteria and dispels miasma but also repels mosquitoes and flies. With improved modern living standards, people sometimes burn pleasant incense in rooms, hotels, or public places, creating a sense of fresh air and an elegant environment. Therefore, with increasing emphasis on aromatherapy and aromatherapy wellness, the future of plant essential oil fumigation is becoming increasingly promising.

[0044] This invention provides a compound composition of plant essential oils as the active ingredient, supplemented with a non-toxic mosquito repellent ingredient as a bactericidal synergist, and further combined with alcohol solvents and essential oil modifiers to obtain a volatile bactericide that is safe for the human body and has no toxic side effects. It can be used to prepare fumigation air disinfectants, which not only meet the requirements for safe use of air disinfectants, but also kill Staphylococcus aureus aerosol and natural bacteria in the air after heating and volatilization, and have a relatively long-lasting bactericidal effect.

[0045] Specifically, this invention allows for the use of less than 1 ml (recommended range 0.5 ml-0.9 ml) in an area of ​​15 m². 2 When used in a room with a ceiling height of 2m, the fumigation solution forms a gaseous state, which comes into full contact with bacteria in the air. People do not need to leave the room. It achieves a kill rate of more than 99% for Staphylococcus aureus aerosol in the air and a natural bacteria elimination rate of more than 95%.

[0046] In practical applications, it can be prepared into electric heating elements, electric incense burners, incense sticks, sanitary incense, coil incense, bamboo incense, or microwave sprays, etc., to meet people's requirements for plant essential oil fumigation. Its application scenarios can be referenced. Figure 1 As shown.

[0047] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto.

[0048] Example 1: Fumigation-type air disinfectant #1

[0049] Raw material components: 7 parts of ethyl 3-(N-butyl-acetamido)propionate, 20 parts of plant essential oil compound composition, 10 parts of ethanol, 0.1 parts of ethyl 2,4-decadienoate and 0.1 parts of butylated hydroxyanisole (BHA).

[0050] In preparation, first mix 1 part peppermint essential oil, 1 part perilla essential oil, 1 part catnip essential oil, 2 parts mugwort essential oil, 1 part clove essential oil, 1 part thyme essential oil, 1 part tea tree essential oil, 1 part cinnamon essential oil, and 2 parts oregano essential oil to obtain a compound composition of plant essential oils. Then mix it with other raw material components to obtain fumigation air disinfectant #1.

[0051] Example 2:

[0052] Raw material composition: 6 parts of ethyl 3-(N-butyl-acetamido)propionate, 20 parts of plant essential oil compound composition, 5 parts of alcohol solvent, 5 parts of phenoxyethanol, 5 parts of n-propanol, 0.8 parts of methyl dihydrojasmone, and 1.5 parts of butylated hydroxyanisole (BHA).

[0053] In preparation, first mix 3 parts peppermint essential oil, 8 parts perilla essential oil, 5 parts catnip essential oil, 10 parts mugwort essential oil, 3 parts clove essential oil, 2 parts thyme essential oil, 5 parts tea tree essential oil, 4 parts cinnamon essential oil, and 6 parts oregano essential oil to obtain a plant-based essential oil compound composition. Then, mix the plant-based essential oil compound composition with other raw material components according to the above proportions to obtain fumigation air disinfectant #2.

[0054] Example 3:

[0055] Raw material composition: 20 parts of ethyl 3-(N-butyl-acetamido)propionate, 50 parts of plant essential oil compound composition, 30 parts of ethanol, 18 parts of phenoxyethanol, 2 parts of n-propanol, 0.1 parts of ethyl 2,4-decadienoate, and 0.1 parts of butylated hydroxyanisole (BHA).

[0056] In preparation, first mix 2 parts peppermint essential oil, 6 parts perilla essential oil, 3 parts catnip essential oil, 10 parts mugwort essential oil, 1 part clove essential oil, 5 parts thyme essential oil, 10 parts tea tree essential oil, 5 parts cinnamon essential oil, and 8 parts oregano essential oil to obtain a plant-based essential oil compound composition. Then, mix the plant-based essential oil compound composition with other raw material components according to the above proportions to obtain fumigation air disinfectant #3.

[0057] Example 4:

[0058] Raw material composition: 6 parts of ethyl 3-(N-butyl-acetamido)propionate, 80 parts of plant essential oil compound composition, 10 parts of ethanol, 10 parts of phenoxyethanol, 10 parts of n-propanol, 1 part of ethyl 2,4-decadienoate, 0.1 parts of methyl dihydrojasmone, and 1 part of butylated hydroxyanisole (BHA).

[0059] In preparation, 5 parts of clove essential oil, 5 parts of thyme essential oil, 5 parts of tea tree essential oil and 5 parts of cinnamon essential oil are first mixed to obtain a compound composition of plant essential oils. Then, the compound composition of plant essential oils is mixed with other raw material components in the above proportions to obtain fumigation air disinfectant #4.

[0060] Example 5:

[0061] Raw material composition: 15 parts of ethyl 3-(N-butyl-acetamido)propionate, 45 parts of plant essential oil compound composition, 25 parts of ethanol, 0.5 parts of methyl dihydrojasmonate, and 1.0 part of butylated hydroxyanisole (BHA).

[0062] In preparation, first mix 3 parts peppermint essential oil, 2 parts perilla essential oil, 4 parts catnip essential oil, 3.5 parts mugwort essential oil, 4.5 parts clove essential oil, 2.2 parts thyme essential oil, 1 part tea tree essential oil, 8 parts cinnamon essential oil, and 4.5 parts oregano essential oil to obtain a plant extract essential oil compound composition. Then, mix the plant extract essential oil compound composition with other raw material components according to the above proportions to obtain fumigation air disinfectant #5.

[0063] Example 6:

[0064] Raw material composition: 12 parts of ethyl 3-(N-butyl-acetamido)propionate, 35 parts of plant essential oil compound composition, 18 parts of ethanol, 10 parts of n-propanol, 0.5 parts of dihydromyrcenol, and 0.5 parts of butylated hydroxyanisole (BHA).

[0065] In preparation, first mix 3 parts peppermint essential oil, 1.5 parts perilla essential oil, 1.5 parts catnip essential oil, 5 parts mugwort essential oil, 3 parts clove essential oil, 5 parts thyme essential oil, 5.5 parts tea tree essential oil, 6 parts cinnamon essential oil, and 7 parts oregano essential oil to obtain a plant-based essential oil compound composition. Then, mix the plant-based essential oil compound composition with other raw material components according to the above proportions to obtain fumigation air disinfectant #6.

[0066] Comparative Example 1:

[0067] This comparative example is a differentiating comparative example from Example 5. It does not use ethyl 3-(N-butyl-acetamido)propionate as in Example 5, but all other components and dosages are the same, resulting in comparative fumigation air disinfectant 1#.

[0068] Comparative Example 2:

[0069] This comparative example is a differentiating comparative example from Example 5. The plant extract essential oil compound composition in Example 5 was not used, but the other components and dosages were the same, resulting in comparative fumigation air disinfectant #2.

[0070] Comparative Example 3:

[0071] This comparative example uses the same raw material components as Example 6, but the proportions are slightly adjusted. Specifically, it consists of 16 parts of ethyl 3-(N-butyl-acetamido)propionate, 8 parts of a compound composition of plant essential oils, 18 parts of ethanol, 12 parts of n-propanol, 0.5 parts of dihydromyrcenol, and 0.5 parts of butylated hydroxyanisole (BHA). The preparation method of the compound composition of plant essential oils involved is the same as that in Example 6, resulting in comparative fumigation air disinfectant #3.

[0072] Comparative Example 4:

[0073] This comparative example is a different comparative example from Example 6, the only difference being the different plant-based essential oil blend used. In this comparative example, the preparation process of the plant-based essential oil blend is as follows:

[0074] Take 5 parts of rosemary essential oil, 5 parts of lemon essential oil, 5 parts of eucalyptus essential oil, 3 parts of grapefruit seed essential oil, 3 parts of litsea cubeba essential oil, 2 parts of geranium essential oil, 4 parts of patchouli essential oil, and 1 part of lemongrass essential oil, and mix them evenly to prepare a compound composition of plant essential oils.

[0075] The above-mentioned fumigation air disinfectants 1#-6# and the comparative fumigation air disinfectants 1#-4# were respectively made into electric heating plates. Cotton pulp paper that meets the technical standards of blank paper plates in Table 1 below was used. Each paper plate had a drip volume of 0.85ml. The plates were sealed with aluminum foil and allowed to diffuse for 24 hours before being used with a heater to conduct the following air disinfection test.

[0076] Table 1. Technical Standards, Test Methods, and Non-conformance Classifications for Blank Paper Sheets

[0077]

[0078]

[0079] I. Air sterilization effect test

[0080] (I) Simulation of field test of air sterilization effect

[0081] Aerosols are gaseous dispersion systems composed of solid or liquid particles suspended in a gaseous medium.

[0082] Test method: at approximately 20m 3 In a room with a temperature of (20-25)℃ and a relative humidity of 50%-70%, the electric heating pad was placed on the matching heater for 120 minutes for disinfection. The content of Staphylococcus albus aerosol in the air of the room area before and after disinfection was measured.

[0083] Staphylococcus aureus in the air of the room area before and after disinfection was sampled using a six-mesh air impact sampler. During sampling, the six-mesh air impact sampler was placed 1m above the center of the room (the sampling method was performed according to the sampler's instruction manual). After sampling, the sampling plates were placed directly into a 37℃ incubator for 48 hours. The final results were observed, the number of colonies was counted, and the airborne bacteria count and kill rate were calculated according to the formula.

[0084] The kill rate is calculated as follows:

[0085]

[0086]

[0087] Where, N t The natural mortality rate of bacteria in the air;

[0088] V0 and V t : These represent the airborne bacterial count at different times before the start of the control group experiment and during the experiment;

[0089] K t The kill rate of bacteria in the air by disinfection treatment;

[0090] V0' and V t ': These represent the airborne bacterial counts at different times before and during the disinfection process in the experimental group.

[0091] The bacterial count in the air before and after disinfection is calculated using the following formula:

[0092]

[0093] (II) Field test of air sterilization effect

[0094] Natural bacteria, in disinfection tests, refer to bacteria that are present on a test subject without artificial contamination.

[0095] Test method: at approximately 30m 3 In a room with a temperature of (20-25)℃ and a relative humidity of 50%-70%, the electric heating element was placed on the matching heater for 120 minutes for disinfection. The content of natural bacteria in the air in the room area before and after disinfection was then measured.

[0096] (1) Based on the actual situation during use, select a representative room and observe the disinfection effect when no one is in the room. During the observation, before disinfection, use a six-mesh air impactor to sample natural bacteria in the air as a pre-disinfection sample (positive control). After disinfection, take another sample as the post-disinfection test sample.

[0097] (2) During sampling, the sampler should be placed 1.0m above the center of the room. (For rooms larger than 10m...) 2 For every additional 10m 2 Add one more sampling point.

[0098] (3) Due to the many variations in the on-site test environment, it is difficult to unify and accurately measure the natural sedimentation rate. Therefore, the verification conclusion is based only on the obtained decay rate (the combined effect of natural decay and sterilization in disinfection treatment).

[0099] The mortality rate is calculated using the following formula:

[0100]

[0101] (4) After the test sampling is completed, the unused culture medium from the same batch should be cultured simultaneously with the above test samples or inoculated and cultured as a negative control. If bacteria grow in the negative control group, it indicates that the culture medium used is contaminated, the test is invalid, and it should be replaced and repeated.

[0102] The results of the air sterilization test of the fumigation-type air sterilization electric heating element (fumigation for 2 hours) are shown in Table 2 below.

[0103] Table 2 Comparison of Air Sterilization Test Results for Fumigation-Type Air Sterilization Electric Heating Arrays

[0104]

[0105]

[0106] Note: The ingredients of commercially available plant essential oil compound antibacterial tablets are listed as peppermint oil, perilla oil, catnip oil and eucalyptus oil.

[0107] As shown in Table 2 above, the electric heating pads made with the fumigation-type air sterilizer of the present invention showed an air sterilization test with a Staphylococcus aureus aerosol kill rate of over 99% and a natural bacteria kill rate of over 95%, which is significantly higher than the test results of commercially available plant essential oil compound sterilization tablets (80.54% and 76.65%). Furthermore, these test results are also higher than those of comparative fumigation-type air sterilizers 1# (90.46% and 88.46%), 2# (90.74% and 85.62%), 3# (92.64% and 87.17%), and 4# (93.35% and 88.42%).

[0108] Compared to the fumigation-type air disinfectant of the present invention, since comparative fumigation-type air disinfectant 1# does not use a compound composition of plant essential oils; comparative fumigation-type air disinfectant 2 does not use ethyl 3-(N-butyl-acetamido)propionate; comparative fumigation-type air disinfectant 3# has a different ratio of the compound composition of plant essential oils and ethyl 3-(N-butyl-acetamido)propionate; and comparative fumigation-type air disinfectant 4# uses other types of compound compositions of plant essential oils, it can be seen that only under the formulation system and ratio range of the fumigation-type air disinfectant provided by the present invention, after compounding and enhancing the effect, can the ideal bactericidal effect be achieved after fumigation and volatilization, that is, the kill rate of Staphylococcus aureus aerosol reaches more than 99%, and the elimination rate of natural bacteria reaches more than 95%.

[0109] The above-mentioned fumigation air disinfectants 1#-6# and control disinfectants 1#-4# were made into incense sticks, lit and used for fumigation, and the following air disinfection tests were conducted (testing method as above).

[0110] The results of the air sterilization test of incense sticks (burned for 2 hours) are shown in Table 3 below.

[0111] Table 3 Comparison of Air Sterilization Test Results of Incense Sticks

[0112]

[0113] As shown in Table 3 above, the incense sticks prepared using the fumigation-type air disinfectant of this invention underwent air disinfection tests. The kill rate of Staphylococcus aureus aerosol was consistently above 99%, and the elimination rate of naturally occurring bacteria was consistently above 95%. These results are higher than those of comparative fumigation-type air disinfectants 1# (90.62% and 85.53%), 2# (90.71% and 85.58%), 3# (92.52% and 87.13%), and 4# (93.21% and 88.34%). Therefore, it can be concluded that only under the formulation system and ratio range of the fumigation-type air disinfectant provided by this invention, and after compounding and synergistic effects, can the ideal disinfection effect be achieved after fumigation and volatilization, i.e., a kill rate of Staphylococcus aureus aerosol exceeding 99% and an elimination rate of naturally occurring bacteria exceeding 95%.

[0114] The above-mentioned fumigation-type air disinfectants 1#-6# and the control fumigation-type air disinfectants 1#-4# were respectively prepared into disinfectant liquids, sprayed and used, and the following air disinfection tests were conducted (at 2ml / m³). 3 (For spraying, the disinfection effect lasts for 60 minutes; all other testing methods are the same.)

[0115] The results of the air sterilization test using sprayed disinfectant are shown in Table 4 below.

[0116] Table 4 Comparison of air sterilization test results using sprayed disinfectant.

[0117]

[0118]

[0119] As shown in Table 4 above, the disinfectant prepared using the fumigation-type air disinfectant of the present invention underwent air disinfection tests. The kill rate of Staphylococcus aureus aerosol was consistently above 99%, and the elimination rate of naturally occurring bacteria was consistently above 95%. These test results are higher than those of comparative fumigation-type air disinfectants 1# (93.43% and 90.15%), 2# (93.46% and 90.34%), 3# (95.76% and 92.33%), and 4# (95.84% and 92.43%). Therefore, the fumigation-type air disinfectant of the present invention can also be used to prepare disinfectant sprays. Under its formulation system and ratio range, after compounding and synergistic effects, it can also achieve ideal disinfection effects, namely, a kill rate of Staphylococcus aureus aerosol in the air exceeding 99% and an elimination rate of naturally occurring bacteria exceeding 95%.

[0120] Electric heating elements prepared with fumigation air sterilizers 5# and 6# were selected as experimental group one and experimental group two, respectively. Electric heating elements prepared with fumigation air sterilizers 1#-4# were selected as control group one, control group two, control group three and control group four, respectively, for air sterilization testing (testing method as above).

[0121] Table 5 Comparison of air sterilization test results of fumigation-type air sterilization electric heating element at different times.

[0122]

[0123]

[0124] As shown in Table 5 above, the heating elements of experimental groups 1 and 2 still have a high sterilization effect after being used for more than 120 minutes. Their Staphylococcus aureus aerosol killing rate is above 99% and the natural bacteria elimination rate is above 95%. Therefore, they have a relatively long-lasting sterilization effect compared with the heating elements of control groups 1 to 4.

[0125] II. Product Toxicological Experiments

[0126] Using the fumigation-type air disinfectant prepared in Example 3, which is formulated with plant essential oils, as a sample, the product was tested for acute inhalation toxicity, acute oral toxicity, and mutagenicity. The results showed that the disinfectant is practically non-toxic.

[0127] (a) Acute inhalation toxicity test

[0128] The fumigation-type air disinfectant prepared in Example 3, which is formulated with plant essential oils, was used as a sample and tested according to the 2002 edition of the "Disinfection Technical Specifications" 2.3.2.

[0129] Animal selection: 20 SPF-grade KM rats, half male and half female, provided by Chengdu Dashuo Experimental Animal Co., Ltd.

[0130] Experimental method: A static exposure method was used. Twenty KM rats, half male and half female, weighing 200g-220g, were selected. The dose was set at 10000mg / m³. 3 One dosage group. Weigh 5.252 g of the original sample solution, heat it to evaporate, and place it in a dosing chamber. Place the rats in the dosing chamber (volume 0.3 ml). 3Animals were exposed to the toxin via a single inhalation for 2 hours. During and after exposure, animals were observed daily for their physical appearance, behavior, respiration, fecal characteristics, genitals, mortality, and other signs of poisoning. If clinical symptoms appeared, the frequency of observation increased. If any animal died from poisoning, the time of death was recorded, its weight was measured, and a necropsy was performed immediately to determine the cause of death. Weight was measured before and 7 days after exposure, and at the end of the treatment. At the end of the two-week observation period, animals were euthanized, and a gross necropsy was performed and observations recorded. The toxicity classification was determined based on the symptoms of poisoning and the number of dead animals.

[0131] Results: 2.118g of the test substance remained after heating; the total amount of exposed liquid consumed after 2 hours of inhalation was 3.134g; the calculated concentration of exposure was 10447mg / m³. 3 After the test substance was heated and exposed to the toxic substance, the animals exhibited normal activity and no abnormal changes were observed. The experimental results indicate that the fumigation-type air disinfectant formulated with plant-derived essential oils is effective against acute inhalation LC50 in SD rats. 50 Greater than 10000 mg / m 3 It is classified as practically non-toxic. The test results are shown in Table 6.

[0132] Table 6. Results of acute inhalation toxicity test on SD rats using a fumigation air disinfectant formulated with plant essential oils (g, ) in Experiment Example 3. )

[0133]

[0134] Note: The letter 'n' represents the number of animals that were alive at that time, and the same applies below.

[0135] As shown in Table 6, the concentration of toxicity in the fumigation air disinfectant prepared in Example 3 using plant-derived essential oils was 10447 mg / m³. 3 Animals in the group who were orally exposed to the disinfectant showed normal activity levels, with no obvious symptoms of poisoning or adverse reactions observed, and no animal deaths were observed during the two-week observation period. The experimental results indicate that this disinfectant is effective against acute inhalation LC50 in SD rats. 50 Greater than 10000 mg / m 3 It is classified as practically non-toxic.

[0136] (II) Acute oral toxicity test

[0137] The fumigation-type air disinfectant prepared in Example 3, which is formulated with plant essential oils, was used as a sample and tested according to the 2002 edition of the "Disinfection Technical Specifications" 2.3.1.

[0138] Animal selection: 20 SPF-grade KM mice, half male and half female, provided by Chengdu Dashuo Experimental Animal Co., Ltd.

[0139] Experimental Method: The Horn's method was used. Twenty KM mice, half male and half female, weighing 19g–22g, were selected. 5mL of the original sample solution was measured and diluted with pure water to 20mL, then mixed thoroughly. A dose group of 5000mg / kg bw was established, and the animals were administered the poison by gavage at a dose of 0.2mL / 10g bw in a single dose while fasting. The animals were observed, their weight measured, dissected, and recorded using the same method as above. The toxicity classification was determined based on the symptoms of poisoning and the number of dead animals. The results are shown in Table 7.

[0140] Table 7. Results of acute oral toxicity test (g) of the fumigation air disinfectant formulated with plant essential oils used in Experiment Example 3 on KM mice. )

[0141]

[0142] Table 7 shows that the animals in the 5000 mg / kg bw dose group of the fumigation air disinfectant solution prepared in Example 3 using plant extract essential oils exhibited normal activity after oral administration, with no obvious symptoms of poisoning or adverse reactions observed, and no animal deaths were observed during the two-week observation period. After the experiment, the surviving animals were euthanized and dissected, and no obvious abnormalities were found in their internal organs. The experimental results indicate that this disinfectant has an acute oral LD50 in KM mice. 50 A concentration greater than 5000 mg / kg bw is considered practically non-toxic.

[0143] (III) Mutagenicity Test

[0144] The fumigation-type air disinfectant prepared in Example 3 using plant-derived essential oils was tested according to section 2.3.8.4 of the 2002 edition of the "Disinfection Technical Specifications".

[0145] Animal selection: 50 SPF-grade KM mice, provided by Chengdu Dashuo Experimental Animal Co., Ltd. Animals underwent acclimatization feeding prior to the experiment.

[0146] Experimental methods: KM mice (weighing 25g-30g) were randomly divided into 5 groups, with 10 mice in each group, half male and half female. The acute oral LD50 of the test substance was referenced. 50 Male rats were given three dose groups of the test substance: 215 mg / kg bw, 860 mg / kg bw, and 2150 mg / kg bw (equivalent to 1 / 2 LD). 50 1 / 5LD 50 and 1 / 20LD 50 Weigh out 0.215 g, 0.860 g, and 2.150 g of the test substance, respectively, and add pure water to 20 mL and mix well. For female mice, three dosage groups of the test substance were established: 158 mg / kg bw, 632 mg / kg bw, and 1580 mg / kg bw (equivalent to 1 / 2 LD). 50 1 / 5LD50 and 1 / 20LD 50 0.158 g, 0.632 g, and 1.58 g of the test substance were weighed, and each was mixed with pure water to a final volume of 20 mL. A negative control (pure water) and a positive control (cyclophosphamide 40 mg / kg bw) were also included. Animals were administered the drug orally at 0 h and 24 h at 0 h and 10 g bw, respectively. Animals were sacrificed 6 h after the last exposure, and slides were prepared according to procedure. 1000 polychromatic erythrocytes (PCEs) were counted from each animal, and the number of PCEs containing micronuclei was observed, and the micronucleus rate (%) was calculated. 200 PCEs were counted, and the proportion of observed mature erythrocytes (NCEs) was also counted, and the PCE / NCE ratio was calculated. The results are shown in Table 8.

[0147] Table 8. Effects of Experiment 3 on the micronucleus incidence of polychromatic erythrocytes in mouse bone marrow.

[0148]

[0149] Note: Dosage "0" is the negative control; compared with the negative control, p < 0.01.

[0150] Table 8 shows that the PCE / NCE ratios of each dose group of the test substance were within 20% of those of the control group, and there was no statistically significant difference (P>0.05), indicating that the test substance had no adverse effect on the proliferation of mouse bone marrow cells. The Poisson distribution test results showed that the micronucleus rate in both male and female animals in the positive control group was significantly higher than that in the negative control group (P<0.01), while there was no significant difference in the micronucleus rate between each group of the test substance and the negative control group (P>0.05), indicating that the test substance yielded negative results in the mouse bone marrow polychromatic erythrocyte micronucleus test.

[0151] Except for Example 3, the other examples also have the same test results as in Example 3. In summary, it shows that the fumigation air disinfectant of the present invention, which uses plant essential oils, has passed the national "Disinfection Technical Specifications" (2002 edition) test. The product is mild, safe and non-irritating.

[0152] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any simple modifications or equivalent changes made to the above embodiments based on the technical essence of the present invention shall fall within the protection scope of the present invention.

Claims

1. A fumigation type air sterilizing agent using a compound of essential oil of a plant, characterized in that: The composition is made by mixing the following components in the following proportions: 3-(N-butyl-acetamido)propionic acid ethyl ester 6-15 parts, plant essential oil compound composition 20-50 parts, alcohol solvent 10-30 parts, essential oil modification blend 0.1-1.1 parts, antioxidant 0.1-1.0 parts, ​ In the fumigation type air sterilizing agent, the weight percentage of the plant essential oil compound composition is more than 20% of the total weight of the fumigation type air sterilizing agent; the weight percentage of the plant essential oil compound composition and the 3-(N-butyl-acetamido)propionic acid ethyl ester is more than 40% of the total weight of the fumigation type air sterilizing agent; the weight ratio of the 3-(N-butyl-acetamido)propionic acid ethyl ester to the plant essential oil compound composition is 1:(2-3), The plant essential oil compound composition is made by mixing the following components in the following proportions: peppermint essential oil 1-3 parts, perilla essential oil 1-8 parts, catnip essential oil 1-5 parts, mugwort leaf essential oil 2-10 parts, clove essential oil 1-5 parts, thyme essential oil 1-5 parts, tea tree essential oil 1-10 parts, cinnamon essential oil 1-8 parts, oregano essential oil 2-8 parts, The alcohol solvent is at least one selected from the group consisting of ethanol, phenoxy ethanol, n-propanol, The essential oil modification blend is at least one selected from the group consisting of 2,4-decadienoic acid ethyl ester, dihydrojasmone, dihydromyrcenol, The antioxidant is butylated hydroxyanisole.

2. The fumigating air sterilizer according to claim 1, wherein: The alcohol solvent contains the following components in the following proportions: ethanol 1-30 parts, phenoxy ethanol 1-20 parts, n-propanol 1-18 parts.

3. A method for preparing a fumigating air sterilizing agent using a compound of essential oil of a plant, characterized in that: The composition is made by mixing the components in the proportions of claim 1.

4. The use of a fumigant air sterilizing agent compounded with essential oil of plant extraction, characterized in that: The fumigation type air sterilizing agent of any one of claims 1-2 is used as an additive for preparing an electric heating sheet, an electric heating incense, a stick incense, a disc incense, a bamboo branch incense, or a microwave spray.

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