A multi-target anti-aging composition and its application in cosmetics

Abstract

This invention discloses a multi-target anti-aging composition and its application in cosmetics. By weight, it comprises the following ingredients: 0.001–3 parts hydroxypinazone retinate, 0.01–5 parts hydroxypropyl tetrahydropyranotriol, 0.01–0.5 parts recombinant human collagen, 0.5–3 parts tocopheryl acetate, 0.1–5 parts glyceryl glucoside, 0.01–5 parts Babao Essence Extract, 0.01–0.5 parts carnosine, 0.5–2 parts 3000 peptides, 0.1–5 parts Bifida ferment lysate, 0.01–0.5 parts coenzyme Q10, and 0.1–5 parts black tea fermentation product. This multi-target anti-aging composition targets four aspects: anti-aging, anti-oxidation, anti-glycation, and anti-photochemical activity. The four categories of components work synergistically to significantly enhance the anti-aging efficacy of the product. Human efficacy evaluation tests show that after using the product of this invention for 28 days, the elasticity of the skin around the eyes is significantly increased, the firmness and roughness of the skin around the eyes are significantly improved, and the area, volume and depth of wrinkles at the corners of the eyes are significantly improved.

Description

Technical Field

[0001] This invention belongs to the field of skincare technology, specifically relating to a multi-target anti-aging composition and its application in cosmetics. Background Technology

[0002] The main manifestations of skin aging include thinning skin, decreased skin elasticity, weakened barrier function, fine lines, wrinkles, and pigmentation. The fundamental mechanisms of skin aging mainly include free radical oxidative stress, glycation, and ultraviolet radiation damaging collagen. The specific mechanisms are as follows: ① Free radicals generated by oxidation damage DNA and accelerate cell death, and also attack cell tissues, leading to collagen loss and damage to the skin barrier; ② Reducing sugars such as glucose and fructose undergo non-enzymatic glycation reactions with collagen and elastin to produce AGEs, altering the normal physical properties of collagen, causing dull skin and loss of elasticity and resilience; ③ UVB and UVA rays in ultraviolet radiation are the inducing factors of photoaging, reaching the epidermis and dermis, inhibiting collagen synthesis, damaging elastin structure, leading to melanin deposition and other skin damage.

[0003] The causes of skin aging can be broadly categorized into two types: endogenous and exogenous aging. Endogenous skin aging refers to the following as we age: ① The function of skin appendages such as sebaceous glands and sweat glands naturally declines, resulting in reduced secretions, which dries out the skin membrane and stratum corneum, causing fine lines and peeling; ② The skin's metabolic capacity declines, leading to the degeneration of elastic fibers and collagen fibers in the dermis, collagen loss, and weakened skin elasticity, making it prone to wrinkles; ③ Active enzymes gradually decrease, and a large number of free radicals damage human tissues and cells, causing various skin problems. Exogenous skin aging includes the effects of external factors such as excessive ultraviolet radiation, harsh climate and environmental pollution, poor lifestyle habits, and excessive work pressure, which cause changes in the physiological form and function of the skin. Among these, photoaging caused by long-term exposure to ultraviolet radiation is the most important exogenous aging, which is essentially skin oxidation caused by excessive free radicals.

[0004] Based on the aforementioned mechanisms of skin aging, the main culprits include four aspects: free radicals, photoaging, cellular glycation, and natural skin aging. Currently, conventional anti-aging products generally only address one or two of these aspects to achieve anti-aging effects, rarely targeting all four dimensions of skin aging. For example, patent application CN111388348A discloses an anti-aging composition that synergistically combines hydroxypinazone retinyl ester, retinyl ester, hydroxypropyl tetrahydropyranotriol, niacinamide, tocotrienol, 3-o-ethyl ascorbic acid, and lipoic acid to achieve superior anti-aging and antioxidant properties. However, this composition primarily focuses on anti-aging and antioxidant effects, and its anti-aging efficacy is limited.

[0005] In addition, many of the better anti-aging ingredients commonly found on the market are highly irritating. For example, retinol, which is often touted as an anti-aging powerhouse, actually needs to be converted into retinoic acid to have a skin-care effect. Retinoic acid is highly irritating and can cause skin intolerance. Bifida ferment lysate produces kojic acid during fermentation, which can also increase skin irritation and cause side effects such as redness and stinging. It is generally not suitable for sensitive skin. Summary of the Invention

[0006] To address the aforementioned technical problems, the present invention aims to propose a multi-target anti-aging composition. This composition utilizes a vitamin A derivative, hydroxypinazone retinate, combined with various active ingredients to address aging from four dimensions: anti-aging, anti-oxidation, anti-glycation, and anti-photoaging. This delays or inhibits free radicals, photoaging, cell glycation, and natural skin aging, significantly improving the product's anti-wrinkle and repair efficacy. Furthermore, the composition of this invention, through the synergistic effect of its various active components, further reduces the irritation of the anti-aging composition, making the resulting product non-irritating and suitable for direct application to the eye area.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a multi-target anti-aging composition, comprising, by weight, the following raw materials:

[0009]

[0010] In the aforementioned technical solution, the structure of hydroxypinazone retinyl ester (HPR) differs from traditional retinoic acid derivatives. Upon decomposition, it directly transforms into retinoic acid, significantly reducing the conversion steps. This means a lower chance of activity loss during conversion, resulting in stronger efficacy stability. Furthermore, HPR is not as irritating as retinoic acid. The transformed retinoic acid is an important hormone-like growth factor that can promote cell proliferation and growth, maintain normal skin circulation, repair lost collagen, and reduce wrinkles and signs of premature aging, thus exhibiting excellent anti-aging effects.

[0011] Hydroxypropyl tetrahydropyranotriol, also known as succinate, is a glycoprotein mixture made from xylose. It promotes collagen synthesis, re-stimulates aging cells, promotes the repair of fine lines, and makes the skin plump, firm, and elastic, thus having a good anti-aging effect.

[0012] Recombinant human collagen, as an active substance, can diffuse into the deep layers of the skin. The tyrosine it contains competes with tyrosine in the skin and binds to the catalytic center of tyrosinase, thereby inhibiting the production of melanin, enhancing the activity of collagen in the skin, maintaining the integrity of the stratum corneum's moisture and fiber structure, promoting skin tissue metabolism, delaying skin aging, restoring and maintaining skin moisture and elasticity, and making the skin smooth and supple, thus having a good anti-aging effect.

[0013] Tocopheryl acetate is a vitamin E derivative with good stability. It is often used as an antioxidant in cosmetics, and has excellent antioxidant effects. It also performs well in moisturizing, maintaining connective tissue, and protecting the skin from UV damage.

[0014] Glyceryl glucoside can increase the production of superoxide dismutase 1 (SOD1), an antioxidant skin-protective enzyme. SOD1 is an antioxidant that is automatically synthesized by the human skin's defense system. It has a good antioxidant effect, which can help reduce skin damage, slow down aging, help repair and soften scar tissue, and protect the skin from ultraviolet damage.

[0015] The Eight Treasures Essence contains extracts of Ligusticum chuanxiong root, Poria cocos sclerotium extract, Atractylodes macrocephala root, Bletilla striata root, Ampelopsis japonica root, artificial bezoar, pearl extract, and borneol. Among these, bezoar has antioxidant properties, borneol has antiseptic and tissue-regenerating effects, and pearl has effects of improving eyesight, calming the mind, relieving wind, and promoting tissue regeneration and wound healing. Combined with Ampelopsis japonica, Bletilla striata, Atractylodes macrocephala, Poria cocos, and Ligusticum chuanxiong, it achieves a good antioxidant effect. The Eight Treasures Essence also has the effect of promoting blood circulation and removing blood stasis, effectively preventing cell damage and repairing the skin barrier, providing good repair and soothing (anti-irritant) effects.

[0016] Black tea fermentation product is a filtrate of acetic acid bacteria / lactobacillus / yeast / tea (Camellia sinensis) leaf extract / sucrose fermentation product. Obtained through the fermentation of black tea, this product significantly inhibits the generation of reactive oxygen species (ROS) in cells, exhibiting excellent antioxidant effects. It also strengthens the epidermal-dermal junction, enhances the activity of damaged tissues, promotes wound healing, and increases epidermal thickness, thereby reducing redness and moisture loss in damaged skin and providing good repair and soothing (anti-irritant) effects.

[0017] Carnosine's structure is similar to the glycosylation sites on protein molecules. By providing an amino group, it can compete with proteins for the binding of glycosyl groups. Carnosine acts as a substitute for proteins in reacting with sugars, thus preventing protein glycation and cross-linking. As a sacrificial protectant, it effectively protects collagen from glycation and has a good anti-glycation effect. Long-term use of skincare products rich in carnosine can protect collagen from glycation, keeping skin firm and radiant.

[0018] 3000 is a complex peptide composed of palmitoyl tetrapeptide-7 and palmitoyl tripeptide-1. Palmitoyl tetrapeptide-7 can delay and inhibit the production of excessive interleukins, thereby inhibiting some unnecessary and inappropriate inflammatory responses and glycation damage. Together with palmitoyl tripeptide-1, they have synergistic effects and also have anti-wrinkle, tightening and lifting functions, and have a good anti-glycation effect.

[0019] Bifida ferment lysate has strong anti-immunosuppressive activity and can promote DNA repair. It can effectively protect the skin from damage caused by ultraviolet rays, resist skin damage caused by ultraviolet rays, help prevent photoaging of the epidermis and dermis, and play a role in stabilizing and repairing, preventing photoaging, and has a very good anti-photochemical effect.

[0020] Coenzyme Q10, also known as ubiquinone, can promote the expression of elastic fibers and type IV collagen by fibroblasts, enhance fibroblast vitality, reduce UV-induced MMP-1 and inflammatory factors produced by keratinocytes, and also help repair and maintain the skin barrier. It can alleviate extrinsic photoaging and inhibit endogenous aging, while also possessing multiple functions such as anti-oxidation, epidermal cell repair, reducing free radicals, and inhibiting melanin production, exhibiting excellent anti-photochemical effects.

[0021] As a preferred embodiment of the technical solution of the present invention, the material comprises the following raw materials in parts by weight:

[0022]

[0023]

[0024] Secondly, the present invention provides a method for preparing the above-mentioned multi-target anti-aging composition, comprising the following steps: adding the formulation amounts of each component to deionized water and mixing them to obtain the final product.

[0025] Thirdly, the present invention provides the application of the multi-target anti-aging composition in cosmetics.

[0026] Preferably, the present invention provides the application of the multi-target anti-aging composition in eye cosmetics.

[0027] Fourthly, the present invention provides a multi-target anti-aging eye cream, prepared from the multi-target anti-aging composition and excipients. That is, in order to improve sensory properties, nourish the skin, and prevent quality deterioration, various ingredients commonly used in eye cosmetics, other than those mentioned above, may be appropriately added to the multi-target anti-aging composition of the present invention, without compromising the effectiveness of the invention.

[0028] Preferably, the excipients of the eye cream are selected from one or more of emulsifiers, polyols, oils, thickeners, chelating agents, preservatives, pH adjusters, and deionized water.

[0029] Preferably, the emulsifier is selected from one or more of cetearyl glucoside, cetearyl alcohol, PEG-100 stearate, cetearyl oleate, and sorbitan oleate.

[0030] Preferably, the polyol is selected from one or more of glycerol, propylene glycol, butylene glycol, sorbitol, dipropylene glycol, 1,3-propanediol, pentanediol, and hexanediol.

[0031] Preferably, the oil is selected from one or more of polydimethylsiloxane, polydimethylsiloxane alcohol, cyclopentadimethylsiloxane, polydimethylsiloxane cross-linked polymer, hydrogenated polyisobutylene, isopropyl myristate, caprylic / capric triglyceride, isononyl isononanoate, neopentyl glycol dicaprylate dicaprylate, and squalane.

[0032] Preferably, the thickener is selected from one or more of the following: polyacrylate crosspolymer-6, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, sorbitan isostearate, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyl taurate / VP copolymer, sodium polyacrylate, polyacrylamide, acrylate / C10-30 alkanol acrylate crosspolymer, and carbomer.

[0033] Further preferably, the thickener comprises polyacrylate crosspolymer-6. By introducing a very small amount of polyacrylate crosspolymer-6 into a conventional thickener, and by introducing a polyelectrolyte polymer with very high associative activity, the thickener can better resist the destructive effects of electrolytes on the formulation, while also possessing excellent suspension stability, thus contributing to improved product stability.

[0034] Preferably, the chelating agent is selected from one or more of disodium EDTA and octanoyl hydroxamic acid.

[0035] Preferably, the preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, chlorphenesin, methylparaben, p-hydroxyacetophenone, propylparaben, 1,2-hexanediol, and sorbitan octanoate.

[0036] Fifthly, the present invention also provides a method for preparing a multi-target anti-aging eye cream, which addresses the following shortcomings:

[0037] (1) The recombinant human collagen was dispersed by stirring with the first part of polyol to obtain the pretreated A1 phase; the first part of thickener was added to the first part of deionized water in the emulsification tank, and after standing, it was homogenized. Then, the temperature was raised to T1℃ and kept warm while stirring. Vacuum was then drawn to remove air bubbles; then the first part of preservative, chelating agent and pretreated A1 phase were added and stirred until the material was completely dissolved.

[0038] (2) Add the oil, emulsifier, tocopheryl acetate and the second part of the preservative to the oil phase pretreatment tank, stir and heat to T2℃, and mix well after dissolving; then filter it into the emulsification tank in step (1), keep it at T2℃ and vacuum homogenize it, and continue to stir until the material is mixed evenly.

[0039] (3) After the thickener in the second part is stirred and dispersed evenly with the polyol in the second part, it is added to the emulsification tank in step (2), vacuum homogenized, stirred until the material is uniform, and then the cooling is turned on.

[0040] (4) After mixing the second part of deionized water, carnosine, and hydroxypinazone retinate, pass the mixture through a 200-mesh sieve and then add it to the emulsion tank material that has been cooled to T3℃. Then, vacuum homogenize and stir until the material is uniform; at the same time, continue to cool down.

[0041] (5) Mix the original liquid of Babao Essence, Bifida Ferment Lysate, Glyceryl Glucoside, Black Tea Fermentation Product, Hydroxypropyl Tetrahydropyrantriol, Peptide 3000, and Coenzyme Q10 and pass them through a 200-mesh sieve; then add them to the emulsion tank material cooled to T4℃, then vacuum homogenize and stir until the material is uniform; at the same time, continue to cool down.

[0042] (6) When the emulsification tank is cooled to T5℃, the third part of the preservative is added to the emulsification tank, vacuum homogenization is performed, and the mixture is stirred until the material is uniform, thus obtaining the multi-target anti-aging eye cream.

[0043] As a preferred technical solution of the present invention, in step (1), T1℃ is 75~85℃;

[0044] In step (2), T2℃ is 75~85℃;

[0045] In step (4), T3℃ is 50~60℃;

[0046] In step (5), T4℃ is 40~50℃;

[0047] In step (6), T5℃ is 35~40℃.

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] (1) The multi-target anti-aging composition of this invention targets four aspects: anti-aging, anti-oxidation, anti-glycation, and anti-photochemical effects. Specifically, it selects hydroxypinazone retinate, hydroxypropyl tetrahydropyranotriol, and recombinant human collagen as anti-aging components; tocopheryl acetate, glyceryl glucoside, Babao essence, and black tea fermentation products as antioxidant components; carnosine and 3000 as anti-glycation components; and Bifida ferment lysate and coenzyme Q10 as anti-photochemical components. The synergistic effect of these four components significantly enhances the anti-aging efficacy of the product. Human efficacy evaluation tests show that after 28 days of using the product of this invention, the elasticity of the skin around the eyes significantly increased, and the firmness and roughness parameters of the skin around the eyes significantly improved. The area, volume, and depth of wrinkles at the corners of the eyes also significantly improved.

[0050] (2) The present invention uses a novel retinol-like hydroxypinazone retinate (HPR). Compared with other vitamin A products, it does not need to undergo metabolic transformation and directly exerts its effects by stimulating receptors. It is less irritating, highly effective, more stable, and less prone to oxidation and inactivation. It is a safe and effective anti-aging ingredient that can effectively reduce fine lines and wrinkles and lighten age spots.

[0051] (3) The main anti-aging components of the multi-target anti-aging composition of this invention, hydroxypinazone retinate and bifida ferment lysate, have certain irritant properties. This invention, by selectively adding Babao essence and black tea fermentation products, not only significantly improves the anti-aging efficacy of the product but also greatly reduces its irritant properties. Chicken embryo tests, acute eye irritation tests, multiple skin irritation tests, and human efficacy evaluation tests demonstrate that the composition of this invention is non-irritating, can be used on the eyes, and has no adverse reactions. Attached Figure Description

[0052] Figure 1 Comparative figures of Example 2 and Example 4 for stability testing.

[0053] Figure 2 The image shows the anti-wrinkle effect on the skin around the eyes of the subjects in Example 4 (VISIA-CR Standard 2 light source mode photo). Detailed Implementation

[0054] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0055] It should be noted that some material information in the embodiments of the present invention is as follows:

[0056] The Eight Treasures Essence used in this embodiment of the invention is produced by Mayinglong Health Industry Co., Ltd., and its preparation method can be referred to the patent document with publication number CN115317429A submitted by the applicant.

[0057] The black tea fermentation product used in this embodiment of the invention is acetic acid bacteria / lactic acid bacteria / yeast / tea (CAMELLIASINENSIS) leaf extract / sucrose fermentation product filtrate, which was purchased from Xianting (Guangzhou) Technology R&D Co., Ltd.

[0058] The recombinant human collagen used in this invention was purchased from Jiangsu Jiangshan Juyuan Biotechnology Co., Ltd.

[0059] The carnosine used in the embodiments of this invention is commercially known as Carnosine.

[0060] The commercial name of the Bifida ferment lysate used in this embodiment of the invention is Repair Complex CLRPF.

[0061] The 3000 peptide used in this embodiment of the invention is marketed as MATRIXYL 3000FREE MBAL.

[0062] The hydroxypinazone retinate used in the embodiments of the present invention is commercially known as NB-668A and was purchased from Guangdong Gexin Biotechnology Co., Ltd.

[0063] The glycerol glucoside used in the embodiments of this invention is commercially known as Annoin.

[0064] The cetearyl oleate / sorbitan oleate used in the embodiments of the present invention is commercially known as Olivem1000.

[0065] The hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer used in the embodiments of this invention is commercially known as SEPINOV. TM WEO.

[0066] The phenoxyethanol / ethylhexylglycerin used in the embodiments of this invention is commercially known as euxyl PE 9010.

[0067] Example 1

[0068] A multi-target anti-aging composition, by weight (g), comprises the following components:

[0069]

[0070] In this embodiment, a method for preparing the above composition is also provided, comprising: adding the prescribed amount of the components to an appropriate amount of deionized water and mixing them to obtain the composition.

[0071] Example 2

[0072] A multi-target anti-aging composition, by weight (g), comprises the following components:

[0073]

[0074] This embodiment also provides a method for preparing the above composition, comprising: adding the prescribed amount of the components to an appropriate amount of deionized water and mixing them to obtain the composition.

[0075] Example 3

[0076] A multi-target anti-aging composition, by weight (g), comprises the following components:

[0077]

[0078]

[0079] This embodiment also provides a method for preparing the above composition, comprising: adding the prescribed amount of the components to an appropriate amount of deionized water and mixing them to obtain the composition.

[0080] Example 4

[0081] A multi-target anti-aging eye cream, by weight (g), is made from the following ingredients:

[0082] Table 1 Raw materials prepared in Example 4

[0083]

[0084]

[0085] In this embodiment, a method for preparing the above-mentioned eye cream is also provided, comprising the following steps:

[0086] (1) Disperse the recombinant human collagen in phase A with 1,3-propanediol to obtain pretreated phase A1, which is ready for use; add the purified water in phase A to an emulsification tank, sprinkle polyacrylate cross-linked polymer-6 evenly on the water surface, let stand for 30 minutes to wet it until there is no floating white powder, stir and homogenize for 10 minutes, slowly raise the temperature to 80°C, keep it warm and stir, remove air bubbles by vacuum, add pretreated phase A1 and other materials in phase A, and stir until all materials are dissolved;

[0087] (2) Add the material in phase B to the oil phase pretreatment tank, stir, heat to 80°C, dissolve completely and mix evenly, then filter it into the emulsification tank, keep it at 80°C, vacuum homogenize for 10 minutes, and continue stirring until the material is evenly mixed.

[0088] (3) After the hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer / sorbitan isostearate in phase C is stirred and dispersed evenly with butanediol, it is added to the emulsification tank, vacuum homogenized for 15 minutes, stirred until the material is uniform, and then the cooling is turned on.

[0089] (4) When the material in the emulsification tank cools down to 55°C, add the D phase material mixture through a 200-mesh sieve into the emulsification tank, vacuum homogenize for 1-5 minutes, and stir until the material is uniform.

[0090] (5) When the material in the emulsification tank cools down to 45°C, add the E phase material mixture through a 200-mesh sieve into the emulsification tank, vacuum homogenize for 2 minutes, and stir until the material is uniform.

[0091] (6) When the material in the emulsification tank cools down to 35°C, add the material in phase F to the emulsification tank, vacuum homogenize for 2 minutes, stir until the material is evenly mixed, stir for 20 minutes, and the multi-target anti-aging eye cream is obtained.

[0092] Example 5

[0093] Table 2 Raw materials prepared in Example 5

[0094]

[0095]

[0096] In this embodiment, a method for preparing the above-mentioned eye cream is also provided, comprising the following steps:

[0097] (1) Disperse the recombinant human collagen in phase A with 1,3-propanediol to obtain pretreated phase A1, which is ready for use; add the purified water in phase A to an emulsification tank, sprinkle polyacrylate cross-linked polymer-6 evenly on the water surface, let stand for 30 minutes to wet it until there is no floating white powder, stir and homogenize for 10 minutes, slowly raise the temperature to 80°C, keep it warm and stir, remove air bubbles by vacuum, add pretreated phase A1 and other materials in phase A, and stir until all materials are dissolved;

[0098] (2) Add the material in phase B to the oil phase pretreatment tank, stir, heat to 80°C, dissolve completely and mix evenly, then filter it into the emulsification tank, keep it at 80°C, vacuum homogenize for 10 minutes, and continue stirring until the material is evenly mixed.

[0099] (3) After the hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer / sorbitan isostearate in phase C is stirred and dispersed evenly with butanediol, it is added to the emulsification tank, vacuum homogenized for 15 minutes, stirred until the material is uniform, and then the cooling is turned on.

[0100] (4) When the material in the emulsification tank cools down to 55°C, add the D phase material mixture through a 200-mesh sieve into the emulsification tank, vacuum homogenize for 1-5 minutes, and stir until the material is uniform.

[0101] (5) When the material in the emulsification tank cools down to 45°C, add the E phase material mixture through a 200-mesh sieve into the emulsification tank, vacuum homogenize for 2 minutes, and stir until the material is uniform.

[0102] (6) When the material in the emulsification tank cools down to 35°C, add the material in phase F to the emulsification tank, vacuum homogenize for 2 minutes, stir until the material is evenly mixed, stir for 20 minutes, and the multi-target anti-aging eye cream is obtained.

[0103] Example 6

[0104] Table 3 Raw materials prepared in Example 6

[0105]

[0106] In this embodiment, a method for preparing the above-mentioned eye cream is also provided, comprising the following steps:

[0107] (1) Disperse the recombinant human collagen in phase A with 1,3-propanediol to obtain pretreated phase A1, which is ready for use; add the purified water in phase A to an emulsification tank, sprinkle polyacrylate cross-linked polymer-6 evenly on the water surface, let stand for 30 minutes to wet it until there is no floating white powder, stir and homogenize for 10 minutes, slowly raise the temperature to 80°C, keep it warm and stir, remove air bubbles by vacuum, add pretreated phase A1 and other materials in phase A, and stir until all materials are dissolved;

[0108] (2) Add the material in phase B to the oil phase pretreatment tank, stir, heat to 80°C, dissolve completely and mix evenly, then filter it into the emulsification tank, keep it at 80°C, vacuum homogenize for 10 minutes, and continue stirring until the material is evenly mixed.

[0109] (3) After the hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer / sorbitan isostearate in phase C is stirred and dispersed evenly with butanediol, it is added to the emulsification tank, vacuum homogenized for 15 minutes, stirred until the material is uniform, and then the cooling is turned on.

[0110] (4) When the material in the emulsification tank cools down to 55°C, add the D phase material mixture through a 200-mesh sieve into the emulsification tank, vacuum homogenize for 1-5 minutes, and stir until the material is uniform.

[0111] (5) When the material in the emulsification tank cools down to 45°C, add the E phase material mixture through a 200-mesh sieve into the emulsification tank, vacuum homogenize for 2 minutes, and stir until the material is uniform.

[0112] (6) When the material in the emulsification tank cools down to 35°C, add the material in phase F to the emulsification tank, vacuum homogenize for 2 minutes, stir until the material is evenly mixed, stir for 20 minutes, and the multi-target anti-aging eye cream is obtained.

[0113] Comparative Example 1

[0114] Compared with Example 4, the only difference is that tocopherol acetate is not added to component B; all other components and preparation methods are the same.

[0115] Comparative Example 2

[0116] Compared with Example 4, the only difference is that polyacrylate crosslinking polymer-6 is not added to component B; all other components and preparation methods are the same.

[0117] Comparative Example 3

[0118] Compared with Example 4, the only difference is that the Eight Treasures Essence stock solution is not added to component E; all other components and preparation methods are the same.

[0119] Comparative Example 4

[0120] Compared with Example 4, the only difference is that the fermented black tea product is not added to component E; all other components and preparation methods are the same.

[0121] Test Example 1: Stability Test

[0122] The high-temperature stability of the eye creams prepared in Examples 4-6 and Comparative Examples 1-2 was investigated. Specifically, the eye creams prepared in Examples 4-6 and Comparative Examples 1-2 were tested at 45°C for 30 days. After all samples returned to room temperature, the physical properties of the products, such as appearance, odor, and texture, were evaluated. Products that showed no obvious discoloration or odor change, no layering of water or oil, and no particles or precipitates were considered stable. At the same time, the residual content of the active ingredient hydroxypinazone retinyl ester (HPR) in the products after 30 days of high-temperature testing was determined by high-performance liquid chromatography. The specific results are shown in Table 4.

[0123] Table 4. Stability test results of the eye creams prepared in the examples and comparative examples.

[0124]

[0125] After being tested at high temperature for one month, the finished product obtained in this embodiment of the invention showed no obvious discoloration or odor change, and the paste did not exhibit any layering phenomena such as water or oil separation. The stability test showed no abnormalities, indicating high product stability. Retinol derivatives have poor light stability, while the residual content of the activator HPR in the product of this embodiment is greater than 90%, indicating high stability of the active ingredients in the product.

[0126] Comparative Example 1, without the addition of the antioxidant tocopheryl acetate, showed a decrease in the residual content of the active ingredient HPR to 71%, indicating poor stability of the active ingredient. This demonstrates that adding an antioxidant to the product of this invention can stabilize the HPR content. Comparative Example 2, after high-temperature environmental testing, exhibited an off-odor, showed precipitates in the ointment, and had a grainy texture upon application, indicating poor product stability. A comparison of the appearances of Comparative Example 2 and Example 4 is shown in the accompanying images. Figure 1 .

[0127] Experimental Example 2: Chicken Embryo Irritation Test

[0128] Chicken embryo irritation tests were conducted on the products prepared in Examples 4-6 and Comparative Examples 3-4.

[0129] Test protocol: Referencing the standard "SN / T 2329-2009 Chicken embryo chorioallantoic membrane test for eye irritation / corrosiveness of cosmetics", 9-day-old SPF grade chicken embryos were selected. 0.3 ml of sample was directly applied to the chorioallantoic membrane (CAM) for 3 min. The paste was then rinsed off with 0.9% saline. Changes in blood vessels were observed after 30 s. The test was repeated with 6 chicken embryos.

[0130] The endpoint evaluation method (ES) was used for evaluation, and the indicators were hemorrhage (H), coagulation (C), and vascularization (L). The degree of occurrence of the above three toxic endpoints was recorded (none: 0 points, mild: 1 point, moderate: 2 points, severe: 3 points). The irritation score ES = hemorrhage (H) + coagulation (C) + vascularization (L) (the mathematical sum of the scores of 6 chicken embryos). The acute eye irritation classification was evaluated according to Table 5, and the irritation results of the product are shown in Table 6.

[0131] Table 5 Evaluation of Endpoint Scoring Method Results

[0132] Finish line score Irritant Classification ES≤12 Non-irritating / mildly irritating 12 < ES < 16 moderate irritation ES≥16 Strongly irritating / corrosive

[0133] Table 6. Irritation results of products obtained from the examples and comparative examples.

[0134] serial number Finish line score (ES) Stimulation intensity Example 4 0 Non-irritating Example 5 0 Non-irritating Example 6 0 Non-irritating Comparative Example 3 5 Mild irritation Comparative Example 4 15 moderate irritation

[0135] As can be seen from Table 6, the eye creams prepared in Examples 4-6 showed no bleeding, coagulation, or vascular dissolution, and were non-irritating. Comparative Example 3 showed mild irritation, and Comparative Example 4 showed moderate irritation. The products prepared in the comparative examples are not suitable for people with thin stratum corneum or sensitive skin.

[0136] Test Example 3: Eye Irritation and Skin Irritation Test

[0137] According to the "Cosmetic Safety Technical Specifications" (2015 edition), the product prepared in Example 4 was used as the test substance, and acute eye irritation test and multiple skin irritation test were conducted respectively.

[0138] 3.1 Acute eye irritation test

[0139] Three healthy adult New Zealand rabbits (standard grade, weighing 2.0–2.5 kg) were selected. The lower eyelid of one rabbit was gently pulled open, and 100 mg of the test substance was applied unchanged into the conjunctival sac. The upper and lower eyelids were then passively closed for 1 second to prevent loss of the test substance. The other eye was left untreated as a self-control. The animals' eyes were examined at 1 hour, 24 hours, 48 ​​hours, and 72 hours after application of the test substance, as well as on days 4 and 7. The experiment was terminated if no irritation reaction was observed after 72 hours. The scores for eye irritation reactions were recorded according to the scoring criteria for eye damage in the *Cosmetic Safety Technical Specifications* (2015 edition). The results are shown in Table 7 below.

[0140] Table 7 Results of the acute eye irritation test on rabbits by the test substances.

[0141]

[0142] Note: The mean of the integrals is rounded to two decimal places.

[0143] As shown in Table 7, the test substance has no acute eye irritation in rabbits under non-rinsing conditions.

[0144] 3.2 Repeated skin irritation tests

[0145] Four healthy adult New Zealand rabbits, of average grade, weighing 2.0–2.5 kg, were used. Hair was removed from both sides of the spine on the back, with each side having a hair removal area of ​​approximately 3 cm × 3 cm. After 24 hours of observation, 0.5 mL of the original test substance was applied to one side of the hairless area of ​​an undamaged rabbit, covering an area of ​​2.5 cm × 2.5 cm. The other side of the hairless area served as a control. Application was performed once daily for 14 consecutive days. Starting from the second day, the hair was clipped before each application, and any remaining test substance was removed with warm water. Skin reactions at the application site were observed 1 hour after removal of the test substance, and skin irritation was scored according to the relevant provisions of the "Cosmetic Safety Technical Specifications" (2015 edition). After the experiment, the total score for 14 days, the average score for each animal over 14 days, and the average score for each animal per day were calculated, and the intensity of skin irritation was graded. The results are shown in Table 8 below.

[0146] Table 8 Results of repeated skin irritation tests on rabbits by the test substances

[0147] Results of repeated skin irritation tests on rabbits by the test substance

[0148]

[0149] Note: The mean of the integrals is rounded to two decimal places.

[0150] As shown in Table 8, the test substance showed no skin irritation to rabbits after repeated exposures.

[0151] Experiment Example 4: Human Efficacy Evaluation Trial

[0152] The present invention uses the eye creams prepared in Example 4 and Comparative Examples 3-4 as test products for human efficacy evaluation experiments.

[0153] 4.1 Test Basis

[0154] The tests were conducted in accordance with the standards T / ZHCA 003-2018. Test method for the effect of cosmetics on transepidermal water loss, T / ZHCA005-2019. Test method for the effect of cosmetics on skin elasticity, and T / ZHCA006-2019. Test method for the anti-wrinkle efficacy of cosmetics.

[0155] 4.2 Evaluation Indicators

[0156] (1) Transdermal water loss

[0157] Measuring instrument: Tewameter TM 300 skin surface moisture loss tester (Courage & Khazaka, Germany).

[0158] Measurement requirements: Perform the test 3 times and take the average value.

[0159] Parameter explanation: The lower the value, the less transepidermal water loss. Unit: g / (h·m 2 ).

[0160] (2) Skin elasticity R2 value

[0161] Measuring instrument: Cutometer MPA580 (Courage & Khazaka, Germany).

[0162] Measurement requirements: Perform the test 3 times and take the average value.

[0163] Parameter explanation: The higher the R2 value, the better the skin elasticity. Unit: dimensionless.

[0164] (3) Wrinkle volume

[0165] Measuring instrument: Derma TOP-V3 rapid optical imaging system for skin (EO-TECH, France).

[0166] Measurement requirements: Acquire one image (front view) and analyze the image once.

[0167] Parameter explanation: The lower the value, the smaller the volume of the wrinkle at the corner of the eye in the test area, in mm. 3 .

[0168] 4.3 Test Methods

[0169] Thirty healthy Chinese subjects aged 30-55 years with some skin elasticity in the left and right corners of their eyes, fine lines or wrinkles, dry and rough skin around the eyes, and a fragile skin barrier were selected and divided into three groups of 10 each. Each group used the test product continuously for 28 days. Testing was conducted on days 14 and 28 of product use. Each subject was tested three times, and the average value was taken. Statistical analysis was then performed using SPSS software.

[0170] After cleansing their face morning and evening, participants should apply an appropriate amount of the product around their eyes until absorbed, replacing their usual facial products. Throughout the testing period, participants must avoid prolonged sun exposure, outdoor sports, travel, etc., and must not use cosmetics or medications with similar effects to the product. Participants must also not change their daily skincare routines during the testing period.

[0171] 4.4 Test Results

[0172] 4.4.1 Results of transepidermal water loss

[0173] The results of the transepidermal water loss test are shown in Table 9. As can be seen from the table, after 14 days of using the product of the embodiment, the transepidermal water loss around the eyes showed a significant reduction. After 28 days of use, the transepidermal water loss further decreased, indicating that the product has a good repairing and soothing effect and works quickly. In contrast, after 14 days of using the comparative product, the improvement in transepidermal water loss around the eyes was not significant. Therefore, it can be concluded that the product of the embodiment of this invention has a far superior repairing effect on the delicate skin around the eyes compared to the comparative product.

[0174] Table 9. Results of transdermal water loss of subjects before and after product use (X±S, n=10)

[0175]

[0176] (Compared with before product use, *P<0.05, **P<0.01)

[0177] 4.4.2 Skin elasticity results

[0178] The results of the R2 test for skin elasticity around the eyes are shown in Table 10. As can be seen from the table, after using the product of the example for 14 days, the R2 value of skin elasticity around the eyes increased significantly. After using the product for 28 days, the R2 value of skin elasticity around the eyes increased further. The product has a good anti-aging effect and works quickly. The comparison shows that the anti-aging effect of the product of the example on the skin around the eyes is better than that of the comparative product.

[0179] Table 10. Skin elasticity R2 test results of subjects before and after product use (X±S, n=10)

[0180]

[0181] (Compared with before product use, *P<0.05, **P<0.01)

[0182] 4.4.3 Wrinkle Volume Results

[0183] The results of the periocular wrinkle volume test are shown in Table 11. As can be seen from the table, the periocular wrinkle volume was significantly reduced after using the product of the embodiment, indicating that the product has a good anti-aging effect. The anti-aging effect of the product of the embodiment of the present invention on the delicate skin around the eyes is better than that of the comparative product.

[0184] Table 11 Results of periorbital wrinkle volume test on subjects' skin before and after product use (X±S, n=10)

[0185]

[0186] (Compared with before product use, *P<0.05, **P<0.01)

[0187] 4.4.4 Subjective Evaluation Results

[0188] After a 28-day trial of the product, participants were asked to complete a questionnaire with options including strongly agree, somewhat agree, neither agree nor disagree, somewhat disagree, and strongly disagree. The percentage of participants who strongly agreed and somewhat agreed was calculated.

[0189] Table 12 Self-assessment feedback on the condition of the skin around the eyes after 28 days of product use.

[0190]

[0191]

[0192] After 28 days of product use, 100% of the subjects were satisfied with the product of Example 4 (including very satisfied and somewhat satisfied), and 100% of the subjects did not experience any discomfort in the eye area during use. 90% of the subjects were satisfied with the product of Comparative Example 3 (including very satisfied and somewhat satisfied), and 90% of the subjects did not experience any discomfort in the eye area during use; one subject experienced mild tingling around the eyes. 70% of the subjects were satisfied with the product of Comparative Example 4 (including very satisfied and somewhat satisfied), and 70% of the subjects did not experience any discomfort in the eye area during use; two subjects experienced mild tingling around the eyes, and one subject experienced tingling around the eyes. Therefore, it can be concluded that the products of the present invention have good efficacy and safety, and high subject satisfaction, while the comparative example products are somewhat irritating and easily cause tingling around the eyes.

[0193] The applicant declares that the present invention illustrates the multi-target anti-aging composition and its application through the above embodiments, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of individual raw materials in the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A multi-target anti-aging eye cream, characterized in that, be prepared from a multi-target anti-aging composition and adjuvants; The multi-target anti-aging composition is composed of the following ingredients: The adjuvants are emulsifiers, polyols, oils, thickening agents, chelating agents, preservatives, pH regulators, and deionized water; The thickening agents are polyacrylate crosspolymer-6, hydroxyethyl acrylate / acryloyldimethyltaurate sodium copolymer, and sorbitan isostearate. The emulsifiers are selected from one or more of cetyl glucoside, cetyl alcohol, PEG-100 stearate, cetyl alcohol olivate, and sorbitan olivate; the polyols are selected from one or more of glycerin, butylene glycol, sorbitol, dipropylene glycol, 1,3-propanediol, pentylene glycol, and hexylene glycol; the oils are selected from one or more of dimethicone, dimethiconol, cyclopentasiloxane, dimethicone crosspolymer, hydrogenated polyisobutene, isopropyl myristate, caprylyl / capryl methacrylate, isononyl isononanoate, neopentyl glycol dicaprylate / dicaprate, and squalane; the chelating agents are selected from one or more of disodium EDTA and caprylyl hydroxysuccinimide; and the preservatives are selected from one or more of phenoxyethanol, ethylhexylglycerin, chlorphenesin, hydroxymethylbenzoate, p-hydroxyacetophenone, hydroxypropyl benzoate, and sorbitan caprylate.

2. A method of preparing the multi-target anti-aging eye cream of claim 1, characterized by, The method comprises the following steps: (1) The recombinant human collagen is dispersed in a first portion of polyol to obtain a pretreated A1 phase; a first portion of thickening agent is added to the first portion of deionized water in an emulsification tank, and then the mixture is homogenized after being left to stand, followed by heating to T1 ℃ and then stirring while maintaining the temperature, and then vacuuming to remove air bubbles; then a first portion of preservative and chelating agent are added to the pretreated A1 phase, and the mixture is stirred until all the ingredients are dissolved; (2) The oils, emulsifiers, tocopheryl acetate, and a second portion of preservative are added to an oil phase pretreatment tank, and then stirred and heated to T2 ℃, and then mixed after the ingredients are dissolved; then the mixture is filtered into the emulsification tank in step (1), and then homogenized while maintaining the temperature at T2 ℃ and vacuuming, and then stirred until the mixture is uniform; (3) A second portion of thickening agent is dispersed in a second portion of polyol, and then added to the emulsification tank in step (2), and then homogenized while vacuuming, and then stirred until the mixture is uniform, and then the temperature is lowered; (4) A second portion of deionized water, carnosine, and hydroxypinacolone retinoate are mixed and then passed through a 200-mesh sieve, and then added to the emulsification tank while the temperature is lowered to T3 ℃, and then homogenized while vacuuming, and then stirred until the mixture is uniform; at the same time, the temperature is continuously lowered; (5) Eight treasures essence stock solution, saccharomyces boulardii ferment lysate, glycerin glucoside, black tea ferment lysate, hydroxypropyl tetrahydro pyrantriol, peptide 3000, and coenzyme Q10 are mixed and then passed through a 200-mesh sieve, and then added to the emulsification tank while the temperature is lowered to T4 ℃, and then homogenized while vacuuming, and then stirred until the mixture is uniform; at the same time, the temperature is continuously lowered; (6) When the temperature of the emulsification tank is lowered to T5 ℃, a third portion of preservative is added to the emulsification tank, and then homogenized while vacuuming, and then stirred until the mixture is uniform, thereby obtaining the multi-target anti-aging eye cream.

3. The preparation method according to claim 2, characterized in that, In step (1), T1 ℃ is 75-85 ℃; In step (2), T2 ℃ is 75-85 ℃; In step (4), T3 ℃ is 50-60 ℃; In step (5), T4°c is 40 to 50°C. In step (6), T5°c is 35 to 40°C.

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