A whitening and anti-aging composition containing a plant cell extract, and a preparation method and application thereof

Abstract

The embodiment of the present specification provides a whitening and anti-aging composition containing plant cell extracts and a preparation method and application thereof, the whitening and anti-aging composition includes the following raw materials in parts by weight: 7-9 parts of rhodiola cell extract, 7-9 parts of ginseng cell extract, 6-8 parts of saussurea cell extract, 5-7 parts of safflower cell extract, 3-5 parts of trans-aminic acid, 0.4-0.6 parts of nicotinamide, 3-5 parts of water-soluble vitamin E, and 0.05-0.2 parts of sodium pyrosulfite. The whitening and anti-aging composition containing plant cell extracts provided by the embodiment of the present specification has stable physical and chemical properties, good preservative performance, and excellent effects of moisturizing, whitening, soothing, and delaying aging.

Description

Technical Field

[0001] This specification relates to the field of skincare technology, and in particular to a whitening and anti-aging composition containing plant cell extracts, its preparation method, and its application. Background Technology

[0002] Skin whitening and anti-aging are popular demands among many skincare consumers, leading to a proliferation of whitening and anti-aging skincare products on the market. Most of these products achieve their whitening and anti-aging effects by adding chemical ingredients. However, excessive use of these chemicals can have side effects on consumers' skin. For example, early whitening active ingredients used in cosmetics included mercury compounds, hormone-like substances, and hydroquinone. These compounds generally acted quickly and had noticeable whitening effects, but they were highly toxic, easily accumulated in the body, causing internal organ failure, or were highly irritating to the skin, potentially leading to cell damage. Their use has been banned in countries worldwide.

[0003] Plant-derived whitening and anti-aging ingredients have advantages in terms of skin irritation and safety, and are more easily accepted by consumers. In recent years, scholars have discovered that some plants or traditional Chinese medicines contain antioxidants that combat free radicals, promote skin metabolism, and delay aging. However, in practical applications, the effects of single plant extracts are limited.

[0004] Therefore, it is desirable to propose a whitening and anti-aging composition containing plant cell extracts, which can achieve good whitening and anti-aging effects through the synergistic effect of various components. Summary of the Invention

[0005] One embodiment of this specification provides a whitening and anti-aging composition containing plant cell extracts. The whitening and anti-aging composition comprises the following raw materials in parts by weight: 7-9 parts of Rhodiola rosea cell extract, 7-9 parts of ginseng cell extract, 6-8 parts of snow lotus cell extract, 5-7 parts of safflower cell extract, 3-5 parts of tranexamic acid, 0.4-0.6 parts of nicotinamide, 3-5 parts of water-soluble vitamin E, 0.05-0.2 parts of sodium metabisulfite, and 52-65 parts of other ingredients.

[0006] In some embodiments, the whitening and anti-aging composition further includes the following raw materials in parts by weight: 0.05-0.2 parts of disodium EDTA; and 0.05-0.15 parts of sodium metabisulfite.

[0007] In some embodiments, the whitening and anti-aging composition comprises: 7.5-8.5 parts of Rhodiola rosea cell extract, 7.5-8.5 parts of ginseng cell extract, 6.5-7.5 parts of Saussurea involucrata cell extract, 5.5-6.5 parts of Carthamus tinctorius cell extract, 3.5-4.5 parts of tranexamic acid, 0.45-0.55 parts of nicotinamide, 3.5-4.5 parts of water-soluble vitamin E, 0.1-0.15 parts of disodium EDTA, and 0.06-0.08 parts of sodium metabisulfite.

[0008] One embodiment of this specification provides a method for preparing a whitening and anti-aging composition containing plant cell extracts, comprising the following steps: Step (1): Take fresh Rhodiola rosea cell culture, ginseng cell culture, snow lotus cell culture, and safflower cell culture, and wash them with pure water; place the washed cell cultures into a homogenizer, add pure water and organic solvent, homogenize, and obtain a homogenate; filter the homogenate using a filter membrane with a pore size of 0.45 μm to obtain a first mixture, wherein the first mixture includes Rhodiola rosea cell extract, ginseng cell extract, and snow lotus cell extract. Lotus cell extract and safflower cell extract; Step (2): Add tranexamic acid, nicotinamide, water-soluble vitamin E and sodium metabisulfite to an emulsifying pot and mix and stir. Add an appropriate amount of water and heat to 35-45°C under continuous stirring to fully dissolve them and obtain a second mixture; Step (3): Put the first mixture and the second mixture into the emulsifying pot and heat to 70-80°C under continuous stirring to homogenize and mix them together. Stir and cool down to 30-40°C and let it age to prepare the whitening and anti-aging composition.

[0009] In some embodiments, the heating temperature in steps (2) and (3) is 73-75°C, and the organic solvent in step (1) is ethanol.

[0010] In some embodiments, the weight ratio of the total weight of the cell culture to the weight of the water in step (1) is 1:5-1:10, and the weight ratio of the cell culture to the weight of the organic solvent is 1:5-1:10.

[0011] In some embodiments, step (1) further includes adding disodium EDTA to the homogenizer, wherein the total weight of the cell extract and the weight ratio of disodium EDTA are 10:1 to 20:1.

[0012] In some embodiments, the weight parts of each component to be added during the preparation of the whitening and anti-aging composition are as follows: 7.5-8.5 parts of Rhodiola rosea cell extract, 7.5-8.5 parts of ginseng cell extract, 6.5-7.5 parts of Saussurea involucrata cell extract, 5.5-6.5 parts of Safflower cell extract, 3.5-4.5 parts of tranexamic acid, 0.45-0.55 parts of nicotinamide, 3.5-4.5 parts of water-soluble vitamin E, 0.1-0.15 parts of disodium EDTA, and 0.06-0.08 parts of sodium metabisulfite.

[0013] One embodiment of this specification provides skincare products containing the whitening and anti-aging composition, including face cream, eye cream, facial cleanser, face mask, BB cream, serum, sunscreen, toner, and lotion.

[0014] One of the embodiments of this specification provides the use of the composition in the preparation of cosmetics for whitening, spot removal, moisturizing, fading spots, smoothing wrinkles, or delaying aging. Detailed Implementation

[0015] As indicated in this specification and claims, unless the context clearly indicates otherwise, the words "a," "an," "an," and / or "the" do not specifically refer to the singular and may also include the plural. Generally speaking, the terms "comprising" and "including" only indicate the inclusion of expressly identified steps and elements, which do not constitute an exclusive list, and the method or apparatus may also include other steps or elements.

[0016] It should be understood that although the terms "first," "second," "third," etc., may be used herein to describe various elements, these elements should not be limited by these terms. These terms are used only to distinguish one element from another. For example, a first mixture may be referred to as a second mixture, and similarly, a second mixture may be referred to as a first mixture without departing from the scope of exemplary embodiments described herein.

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains.

[0018] Some embodiments of this specification provide a whitening and anti-aging composition containing plant cell extracts, wherein the whitening and anti-aging composition comprises the following raw materials in parts by weight: 7-9 parts of Rhodiola rosea cell extract, 7-9 parts of ginseng cell extract, 6-8 parts of Saussurea involucrata cell extract, 5-7 parts of Safflower cell extract, 3-5 parts of tranexamic acid, 0.4-0.6 parts of nicotinamide, 3-5 parts of water-soluble vitamin E, 0.05-0.2 parts of sodium metabisulfite, and 52-65 parts of other ingredients.

[0019] Rhodiola rosea cell extract stimulates the division and proliferation of fibroblasts in the dermis, promoting fibroblast division and the synthesis and secretion of collagen. It also stimulates cells to secrete collagenase, causing the breakdown of existing collagen. The collagen then forms collagen fibers outside the cells, thus increasing the amount of collagen fibers and exhibiting a strong anti-aging effect. Furthermore, Rhodiola rosea cell extract can inhibit tyrosine kinase activity, thereby preventing melanin deposition and the formation of dark brown spots, resulting in a whitening effect.

[0020] In some embodiments, the Rhodiola rosea cell extract may be present in 7-8 parts by weight in the whitening and anti-aging composition. In some embodiments, the Rhodiola rosea cell extract may be present in 8-9 parts by weight in the whitening and anti-aging composition. In some embodiments, the Rhodiola rosea cell extract may be present in 7, 7.5, 8, 8.5, or 9 parts by weight in the whitening and anti-aging composition. Preferably, the Rhodiola rosea cell extract may be present in 8 parts by weight in the whitening and anti-aging composition.

[0021] Ginseng cell extract contains various ginsenosides, amino acids, vitamins, and minerals. It promotes blood circulation in subcutaneous capillaries, strengthens cell vitality, and stimulates collagen production, effectively delaying aging. Furthermore, it has excellent permeability, deeply moisturizing the skin, providing nourishment, and quickly regulating skin moisture balance. For example, ginsenosides can disrupt the transfer of melanin from basal melanocytes to upper keratinocytes, inhibiting the transfer of melanosomes to keratinocytes, thus exhibiting good whitening effects.

[0022] In some embodiments, the ginseng cell extract may comprise 7-8 parts by weight in the whitening and anti-aging composition. In some embodiments, the ginseng cell extract may comprise 8-9 parts by weight in the whitening and anti-aging composition. In some embodiments, the ginseng cell extract may comprise 7.5-8.5 parts by weight in the whitening and anti-aging composition. In some embodiments, the ginseng cell extract may comprise 7, 7.5, 8, 8.5, or 9 parts by weight in the whitening and anti-aging composition. Preferably, the ginseng cell extract may comprise 8 parts by weight in the whitening and anti-aging composition.

[0023] For a comparison of the synergistic anti-aging effects of ginseng cell extracts, please refer to the anti-aging effect evaluation results of Comparative Examples 1-2 and Example 1-1.

[0024] Snow lotus cell extract contains snow lotus lactones and snow lotus polysaccharides, which have anti-inflammatory, tissue-repairing, and superoxide free radical scavenging effects. It is also rich in proteins and amino acids, which can regulate the pH balance of the skin surface, helping to improve the skin's pH level and maintain the skin's microecological balance. Furthermore, the abundant vitamin C in snow lotus cell extract has a strong antioxidant effect, which can soften blood vessels, increase vascular elasticity, and help delay skin aging.

[0025] In some embodiments, the snow lotus cell extract may be 6-7 parts by weight in the whitening and anti-aging composition. In some embodiments, the snow lotus cell extract may be 7-8 parts by weight in the whitening and anti-aging composition. In some embodiments, the snow lotus cell extract may be 6, 6.5, 7, 7.5, or 8 parts by weight in the whitening and anti-aging composition. Preferably, the snow lotus cell extract may be 7 parts by weight in the whitening and anti-aging composition.

[0026] For a comparison of the synergistic whitening and anti-aging effects of snow lotus, please refer to the anti-aging effect evaluation results of Comparative Example 1-1 and Example 1-1.

[0027] Hydroxysafflower yellow A in safflower cell extract has anti-photoaging (extrinsic aging) properties. It can indirectly and / or directly regulate the activity of matrix metalloproteinases, thereby inhibiting collagen degradation and delaying aging. Safflower cell extract also contains flavonoids, vitamin E, and other antioxidants, which can play a certain defensive role against skin aging and promote blood circulation.

[0028] The safflower yellow pigment and safflower flavonoids in safflower cell extract can inhibit the activity of tyrosinase, thereby achieving a whitening effect.

[0029] In some embodiments, the safflower cell extract may be present in 5-6 parts by weight in the whitening and anti-aging composition. In some embodiments, the safflower cell extract may be present in 6-7 parts by weight in the whitening and anti-aging composition. In some embodiments, the safflower cell extract may be present in 5.5, 6, or 6.5 parts by weight in the whitening and anti-aging composition. Preferably, the safflower cell extract may be present in 6 parts by weight in the whitening and anti-aging composition.

[0030] In some embodiments, the remaining components of the whitening and anti-aging composition include the following raw materials in parts by weight: 0.05-0.2 parts of disodium EDTA, wherein the amount of sodium metabisulfite is 0.05-0.1 parts.

[0031] In some embodiments, the sodium metabisulfite in the whitening and anti-aging composition may be 0.05, 0.08, or 0.1 parts by weight. Preferably, the sodium metabisulfite in the whitening and anti-aging composition is 0.1 parts by weight.

[0032] Disodium EDTA can chelate metal ions. In one or more embodiments of this specification, disodium EDTA is added to the whitening and anti-aging composition formulation. After dissolving in water, it binds firmly to metal ions, making it difficult for them to undergo normal valence reactions, thereby maintaining the stability of the product and reducing the damage of harmful metal ions to the skin. In some embodiments, the disodium EDTA in the whitening and anti-aging composition may be 0.05-0.15 parts by weight. In some embodiments, the disodium EDTA in the whitening and anti-aging composition may be 0.1-0.15 parts by weight. In some embodiments, the disodium EDTA in the whitening and anti-aging composition may be 0.05, 0.1, 0.12, or 0.15 parts by weight. Preferably, the disodium EDTA in the whitening and anti-aging composition is 0.12 parts by weight.

[0033] In some embodiments, the whitening and anti-aging composition includes: 7.5-8.5 parts of Rhodiola rosea cell extract, 7.5-8.5 parts of ginseng cell extract, 6.5-7.5 parts of Saussurea involucrata cell extract, 5.5-6.5 parts of Carthamus tinctorius cell extract, 3.5-4.5 parts of tranexamic acid, 0.45-0.55 parts of nicotinamide, 3.5-4.5 parts of water-soluble vitamin E, 0.1-0.15 parts of disodium EDTA, and 0.06-0.08 parts of sodium metabisulfite.

[0034] In some embodiments, the whitening and anti-aging composition may include: 8 parts of Rhodiola rosea cell extract, 8 parts of ginseng cell extract, 7 parts of snow lotus cell extract, 6 parts of safflower cell extract, 5 parts of tranexamic acid, 0.5 parts of nicotinamide, 4 parts of water-soluble vitamin E, 0.1 parts of disodium EDTA, and 0.1 parts of sodium metabisulfite.

[0035] This specification provides a method for preparing a whitening and anti-aging composition containing plant cell extracts, comprising the following steps: Step (1): Take fresh Rhodiola rosea cell culture, ginseng cell culture, snow lotus cell culture, and safflower cell culture, and wash them with pure water; place the washed cell cultures into a homogenizer, add pure water and organic solvent, homogenize, and obtain a homogenate; filter the homogenate using a filter membrane with a pore size of 0.45 μm to obtain a first mixture, wherein the first mixture includes Rhodiola rosea cell extract, ginseng cell extract, and snow lotus cell extract. Lotus cell extract and safflower cell extract; Step (2): Add tranexamic acid, nicotinamide, water-soluble vitamin E and sodium metabisulfite to an emulsifying pot and mix and stir. Add an appropriate amount of water and heat to 35-45°C under continuous stirring to fully dissolve them and obtain a second mixture; Step (3): Put the first mixture and the second mixture into the emulsifying pot and heat to 70-80°C under continuous stirring to homogenize and mix them together. Stir and cool down to 30-40°C and let it age to prepare the whitening and anti-aging composition.

[0036] In some embodiments, step (1) further includes adding disodium EDTA to the homogenizer, wherein the total weight of the cell extract and the weight ratio of disodium EDTA are 10:1 to 20:1.

[0037] In some embodiments, the weight parts of each component to be added during the preparation of the whitening and anti-aging composition are as follows: 7.5-8.5 parts of Rhodiola rosea cell extract, 7.5-8.5 parts of ginseng cell extract, 6.5-7.5 parts of Saussurea involucrata cell extract, 5.5-6.5 parts of Safflower cell extract, 3.5-4.5 parts of tranexamic acid, 0.45-0.55 parts of nicotinamide, 3.5-4.5 parts of water-soluble vitamin E, 0.1-0.15 parts of disodium EDTA, and 0.06-0.08 parts of sodium metabisulfite.

[0038] In one or more embodiments of this specification, by adding disodium EDTA during the extraction process of plant extracts, the yield of various plant extracts can be increased, the solubility of active ingredients in aqueous solutions can be improved, and thus the plant extracts can be fully utilized.

[0039] In some embodiments, the heating temperature in steps (2) and (3) is 73-75°C, and the organic solvent in step (1) is ethanol.

[0040] One or more embodiments of this specification can reduce ethanol residue in the composition by adjusting the heating temperature to 73-75°C.

[0041] In some embodiments, the weight ratio of the total weight of the cell culture to the weight of the water in step (1) is 1:5-1:10, and the weight ratio of the cell culture to the weight of the organic solvent is 1:5-1:10.

[0042] In some embodiments, the cell culture may be 29 parts by weight and the water may be 145 parts by weight.

[0043] This specification provides a skincare product containing the whitening and anti-aging composition, including face cream, eye cream, facial cleanser, face mask, BB cream, serum, sunscreen, toner, and lotion.

[0044] This specification provides, on the one hand, the application of the whitening and anti-aging composition in the preparation of cosmetics for whitening, removing blemishes, moisturizing, fading spots, smoothing wrinkles, or delaying aging.

[0045] The following embodiments are more specific descriptions of embodiments related to the above-described embodiments. Some content in these embodiments may be replaced or combined with corresponding content in other embodiments to form new embodiments. Unless otherwise specified, the experimental methods in the following embodiments are conventional methods. Unless otherwise specified, the experimental materials used in the following embodiments were purchased from conventional biochemical reagent companies. The quantitative experiments in the following embodiments were all performed in triplicate, and the results were averaged. It should be understood that the following embodiments are for better explanation of the present invention and are not intended to limit the present invention.

[0046] Example

[0047] Methods for preparing plant cell extracts

[0048] Fresh Rhodiola rosea cell cultures, safflower cell cultures, ginseng cell cultures, and Saussurea involucrata cell cultures were washed with purified water. The washed cell cultures were then placed in a homogenizer, and purified water, ethanol, and disodium EDTA were added. The total weight ratio of the cell extract to disodium EDTA was 10:1-20:1. The total weight ratio of the cell cultures to water was 1:5-1:10, and the weight ratio of the cell cultures to ethanol was 1:5-1:10. The above components were homogenized to obtain a homogenate. The homogenate was filtered through a 0.45 μm filter membrane, and then distilled under reduced pressure to obtain the plant cell extract.

[0049] First set of embodiments

[0050] 1. Raw material composition

[0051] Table 1 Raw Material Composition Table

[0052]

[0053]

[0054] 2. Preparation method

[0055] Step 1: Take Rhodiola rosea cell extract, ginseng cell extract, snow lotus cell extract and safflower cell extract according to the proportions shown in Table 1, mix them well to obtain the first mixture;

[0056] Step 2: Add tranexamic acid, nicotinamide, water-soluble vitamin E, disodium EDTA, and sodium metabisulfite to an emulsifying pot and mix. Add an appropriate amount of water and heat to 35-45°C while stirring continuously to fully dissolve the mixture and obtain the second mixture.

[0057] Step 3: Place the first mixture and the second mixture into the emulsification pot, heat to 74°C under continuous stirring, and homogenize them together; stir and cool down to 30-40°C, let it age, and prepare the whitening and anti-aging composition.

[0058] 3. Effectiveness Evaluation

[0059] (1) Whitening test

[0060] 1) Cell seeding: at 3×10 5 Human melanocytes were seeded into 6-well cell culture plates at a seeding density of cells / well and incubated overnight in an incubator (37°C, 5% CO2).

[0061] 2) Sample loading: When the cell seeding rate in the 6-well plate reaches 40% to 60%, the samples of the example and comparative examples are added in groups. The sample loading volume is 2 mL per well, and each group has 3 replicates. The cells are incubated in an incubator (37℃, 5% CO2) for 72 h. After 72 h, the culture medium is discarded and the cells are washed once with PBS.

[0062] 3) Cell digestion: Digest melanocytes with 0.25% trypsin (700 μL / well) at 37℃ for 1-2 min. Observe under a microscope. When 80% of the cells have shrunk into spherical shape and have not detached from the attachment surface, immediately add 700 μL / well of DMEM containing 10% FBS to stop the reaction. After pipetting and mixing, collect the cells into 1.5 ml EP tubes, centrifuge at 10000 r / min for 10 min, and discard the supernatant.

[0063] 4) Prepare a mixture of distilled water, anhydrous ethanol and diethyl ether in a 2:5:5 ratio in a fume hood. After mixing well, add 1.2 mL / tube to an EP tube and shake vigorously until the opaque substance in the EP tube disappears. After standing at room temperature for 30 min, centrifuge at 10000 r / min for 10 min.

[0064] 5) Discard the supernatant, add 1 mL of 1 mol / L NaOH aqueous solution containing 10% (volume fraction) DMSO, and seal with sealing film to ensure tightness;

[0065] 6) Place the sample and melanin standard solutions of different concentrations in an 80°C water bath and heat for 30 minutes respectively;

[0066] 7) After heating, once the temperature has reached room temperature, add 200 μL of the above solution to each well of the 96-well plate. Set up two replicates for each EP tube and measure the absorbance (OD) value at a wavelength of 405 nm using a microplate reader.

[0067] 8) Plot a standard curve with the OD values ​​of standard solutions of different concentrations on the x-axis and the melanin content on the y-axis, obtain the regression equation, substitute the sample OD values ​​into the formula, and calculate the melanin content of the sample group.

[0068] (2) Anti-aging test

[0069] One of the main causes of skin aging is the alteration of the structure of the dermis. Due to external factors, matrix metalloproteinases (MMPs) in the body are activated, leading to the excessive degradation of collagen and elastin, which support the skin structure in the dermis, resulting in signs of aging such as wrinkles and loss of elasticity.

[0070] MMP-1, also known as collagenase-1, can degrade type III collagen. The anti-aging effect of a sample can be evaluated by testing its inhibition rate of MMP-1.

[0071] The testing steps are as follows:

[0072] 1) Seed fibroblasts into 12-well cell culture plates, with each well containing 0.75 × 10⁶ cells. 5 Cells were starved in serum-free medium for 24 hours.

[0073] 2) Rinse the starved cells with PBS and treat them with ultraviolet light (40 mJ).

[0074] 3) Add the test sample to the cells twice within 48 hours. Measure the isolated MMP-1 in the culture medium using a kit (BIOTRAK, RPN2610).

[0075] 4) The anti-aging efficacy is assessed by calculating the MMP-1 expression inhibition rate. The calculation formula is as follows:

[0076] Inhibition rate = (AB) / A * 100%

[0077] A: No test sample was added, but the expression level of MMP-1 after UV irradiation was [not specified].

[0078] B: MMP-1 expression level after adding test samples and irradiating with UV light

[0079] (3) Anti-corrosion effect test

[0080] 1) The samples from the examples and the comparative examples were left to stand for 60 days. Microbiological testing was performed according to GB / T7918.2—1987, the standard test method for microorganisms in cosmetics.

[0081] (4) Emulsification effect test

[0082] Heat resistance stability test: The sample was incubated in a constant temperature incubator at 48 degrees Celsius until precipitation occurred, and the time it took for precipitation to occur was recorded.

[0083] (5) Moisturizing effect

[0084] Transdermal water loss (TEWL), also known as transdermal water loss, represents the loss of moisture from the deep dermis through evaporation from the epidermis. It is an important parameter describing the skin barrier and is closely related to the water content of the stratum corneum. A higher TEWL value indicates greater water loss through the skin; therefore, the rate of increase or decrease in TEWL value can be used to measure the moisturizing effect of a sample. The smaller the rate of increase or decrease in TEWL value, the better the moisturizing effect of the sample.

[0085] TEWL value increase / decrease rate = (TEWL value after sample use - TEWL value before use) ÷ TEWL value before use × 100%.

[0086] 1) Ten volunteers with the same facial skin moisture content were selected. The ten volunteers were randomly divided into groups of five. Samples prepared by the example and comparative examples were randomly selected.

[0087] 2) The volunteer's left cheek was treated with the sample; the right cheek was not treated and served as a blank control group. The sample was used twice a day, morning and evening.

[0088] 3) Measure the transepidermal water loss rate of the face using the Tewameter probe before product use and 4 weeks after use. Measure the same point 3 times and take the average value.

[0089] 4) Calculate the increase or decrease rate of the TEWL value of the sample based on the measured transepidermal water loss rate of the face.

[0090] 4. Results Recording

[0091] Table 2 Evaluation Results

[0092]

[0093]

[0094] Table 2 shows that the anti-aging and moisturizing effects of Comparative Example 1-1, which lacks the component of snow lotus cell extract, are lower than those of other samples, indicating that snow lotus has a synergistic anti-aging and moisturizing effect in the whitening and anti-aging composition. Similarly, the anti-aging and moisturizing effects of Comparative Example 1-2, which lacks the component of ginseng cell extract, are lower than those of other samples, indicating that ginseng also has a synergistic anti-aging and moisturizing effect in the whitening and anti-aging composition. The MMP-1 inhibition rate tested using samples from Examples 1-1 and 1-2 is relatively high. The product exhibits a relatively long period of stability without precipitation and the lowest TEWL value increase / decrease rate, indicating that when the whitening and anti-aging composition contains 7-9 parts of Rhodiola rosea cell extract, 7-9 parts of ginseng cell extract, 6-8 parts of snow lotus cell extract, 5-7 parts of safflower cell extract, 3-5 parts of tranexamic acid, 0.4-0.6 parts of nicotinamide, 3-5 parts of water-soluble vitamin E, and 0.05-0.2 parts of sodium metabisulfite, its physicochemical properties are stable, and it possesses excellent moisturizing, whitening, soothing, and anti-aging effects.

[0095] Second set of embodiments

[0096] 1. Raw material composition

[0097] Table 3 Raw Material Composition Table

[0098]

[0099]

[0100] 2. Preparation method

[0101] Step 1: Take the Rhodiola rosea cell extract, ginseng cell extract, snow lotus cell extract and safflower cell extract according to the proportions shown in Table 3, put them into a homogenizer and mix well to obtain the first mixture.

[0102] Step 2: Add tranexamic acid, nicotinamide, water-soluble vitamin E, disodium EDTA (add other preservatives according to Table 5 in the comparative proportion), and sodium metabisulfite to an emulsifying pot and mix. Add an appropriate amount of water and heat to 35-45℃ under constant stirring to fully dissolve the mixture and obtain the second mixture.

[0103] Step 3: Place the first mixture and the second mixture into the emulsification pot, heat to 74°C under continuous stirring, and homogenize them together; stir and cool down to 30-40°C, let it age, and prepare the whitening and anti-aging composition.

[0104] 3. Effectiveness Evaluation

[0105] For specific test steps, please refer to the first set of examples.

[0106] 4. Results Recording

[0107] Table 4 Evaluation Results

[0108]

[0109]

[0110] Table 4 shows that the sample in Example 2-2 exhibited the best preservative effect and required a small amount of sodium metabisulfite, demonstrating that disodium EDTA can reduce the amount of sodium metabisulfite while simultaneously enhancing the preservative effect. The whitening, anti-aging, and moisturizing effects of the samples in Examples 2-1 and 2-2 were superior to those in Examples 2-3, Comparative Example 2-1, and Comparative Example 2-2, proving that 0.0-0.2 parts of disodium EDTA not only provides excellent preservative effects but also enhances the whitening and anti-aging effects of the samples. Furthermore, compared to Examples 1-1 and 1-2, the addition of disodium EDTA to the whitening and anti-aging compositions in Examples 2-1 and 2-2 significantly increased the duration of stability without precipitation and the MMP-1 inhibition rate. This indicates that disodium EDTA improves the anti-aging effect of the whitening and anti-aging compositions. In addition, the test results of Comparative Examples 2-1 and 2-2 also show that although traditional methylparaben and phenoxyethanol can be used in combination with sodium metabisulfite to achieve a synergistic anti-corrosion effect, the maximum duration for which their anti-aging and moisturizing effects are stable and do not produce precipitation is much shorter than that of the disodium EDTA group (i.e., Examples 2-1 to 2-3).

[0111] Third set of embodiments

[0112] 1. Raw material composition

[0113] Table 5 Raw Material Composition Table

[0114]

[0115]

[0116] 2. Preparation method

[0117] Step 1: Take the Rhodiola rosea cell extract, ginseng cell extract, snow lotus cell extract and safflower cell extract according to the proportions shown in Table 5, put them into a homogenizer and mix well to obtain the first mixture.

[0118] Step 2: Add tranexamic acid, nicotinamide, water-soluble vitamin E, disodium EDTA, and sodium metabisulfite to an emulsifying pot and mix. Add an appropriate amount of water and heat to 70-80°C while stirring continuously to fully dissolve the mixture and obtain the second mixture.

[0119] Step 3: Place the first mixture and the second mixture into the emulsification pot, heat to 74°C under continuous stirring, and homogenize them together; stir and cool down to 30-40°C, let it age, and prepare the whitening and anti-aging composition.

[0120] 3. Effectiveness Evaluation

[0121] For specific test steps, please refer to the first set of examples.

[0122] 4. Results Recording

[0123] Table 6 Evaluation Results

[0124]

[0125] Table 6 shows that the whitening effect, anti-aging effect, anti-corrosion effect, emulsification effect and moisturizing effect of the sample in Example 3-3 are the best overall, thus the composition ratio of Example 3-3 is the optimal ratio.

[0126] Fourth set of embodiments

[0127] 1. Raw material composition

[0128] Table 7 Raw Material Composition Table

[0129]

[0130] 2. Preparation method

[0131] Step 1: Take the Rhodiola rosea cell extract, ginseng cell extract, snow lotus cell extract and safflower cell extract according to the proportions shown in Table 7, put them into a homogenizer and mix well to obtain the first mixture.

[0132] Step 2: Add tranexamic acid, nicotinamide, water-soluble vitamin E, disodium EDTA, and sodium metabisulfite to an emulsifying pot and mix. Add an appropriate amount of water and heat to 40°C while stirring continuously to fully dissolve the mixture and obtain the second mixture.

[0133] Step 3: Place the first mixture and the second mixture into the emulsification pot, heat to 65°C (85°C for Comparative Example 3-2) under continuous stirring, and homogenize them together; stir and cool to 30-40°C, let it age, and prepare the whitening and anti-aging composition.

[0134] 3. Effectiveness Evaluation

[0135] For specific test steps, please refer to the first set of examples.

[0136] 4. Results Recording

[0137] Table 8 Evaluation Results

[0138]

[0139]

[0140] As shown in Table 8, in the test results of Comparative Example 3-1 (mixing temperature reduced to 65℃ in step (3)) and Comparative Example 3-2 (mixing temperature increased to 85℃ in step (3)), the melanin content and MMP-1 inhibition rate were both lower than those of Example 3-3 (mixing temperature of 74℃ in step (3)). This indicates that excessively high mixing temperature in step (3) may lead to the inactivation of plant components, while excessively low temperature will affect the emulsification effect and efficacy of the whitening and anti-aging composition. At 74℃, the obtained whitening and anti-aging composition has stable physicochemical properties, good preservative properties, and excellent moisturizing, whitening, soothing, and anti-aging effects.

[0141] Those skilled in the art should understand that the above embodiments are merely illustrative of the present invention and do not constitute a limitation thereof. Any modifications, equivalent substitutions, and variations made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

[0142] Furthermore, this specification uses specific terms to describe embodiments thereof. For example, "an embodiment," "one embodiment," and / or "some embodiments" refer to a particular feature, structure, or characteristic associated with at least one embodiment of this specification. Therefore, it should be emphasized and noted that references to "an embodiment," "one embodiment," or "an alternative embodiment" in different locations throughout this specification do not necessarily refer to the same embodiment. Moreover, certain features, structures, or characteristics in one or more embodiments of this specification can be appropriately combined.

[0143] In some embodiments, numbers describing the quantity of components and attributes are used. It should be understood that such numbers used in the description of embodiments are modified in some examples with the terms "approximately," "approximately," or "generally." Unless otherwise stated, "approximately," "approximately," or "generally" indicates that the numbers are allowed to vary by ±20%. Accordingly, in some embodiments, the numerical parameters used in the specification and claims are approximate values, which may be changed depending on the characteristics required by individual embodiments. In some embodiments, numerical parameters should take into account specified significant digits and employ a general method of digit reservation. Although the numerical ranges and parameters used to confirm their breadth of range in some embodiments of this specification are approximate values, in specific embodiments, such values ​​are set as precisely as feasible.

[0144] For each patent, patent application, patent application publication, and other material, such as articles, books, specifications, publications, and documents, referenced in this specification, the entire contents of which are incorporated herein by reference. This excludes historical application documents that are inconsistent with or conflict with the content of this specification, as well as documents that limit the broadest scope of the claims in this specification (currently or subsequently appended to this specification). It should be noted that in the event of any inconsistency or conflict between the descriptions, definitions, and / or terminology used in the supplementary materials to this specification and the content of this specification, the descriptions, definitions, and / or terminology used in this specification shall prevail.

[0145] Finally, it should be understood that the embodiments described in this specification are merely illustrative of the principles of the embodiments described herein. Other variations may also fall within the scope of this specification. Therefore, alternative configurations of the embodiments described herein are intended to be illustrative rather than limiting, and should be considered consistent with the teachings of this specification. Accordingly, the embodiments described herein are not limited to those explicitly introduced and described herein.

Claims

1. A whitening and anti-aging composition containing plant cell extracts, characterized in that, The whitening and anti-aging composition comprises the following raw materials in parts by weight: 7-9 parts of Rhodiola rosea cell extract, 7-9 parts of ginseng cell extract, 6-8 parts of snow lotus cell extract, 5-7 parts of safflower cell extract, 3-5 parts of tranexamic acid, 0.4-0.6 parts of nicotinamide, 3-5 parts of water-soluble vitamin E, 0.05-0.2 parts of sodium metabisulfite, 0.05-0.2 parts of disodium EDTA, and 52-65 parts of the remaining ingredients; The preparation method of the whitening and anti-aging composition includes the following steps: Step (1): Take fresh Rhodiola rosea cell culture, ginseng cell culture, snow lotus cell culture, and safflower cell culture, and wash them with pure water; put the washed cell cultures into a homogenizer, add pure water, disodium EDTA, and an organic solvent, and homogenize to obtain a homogenate; filter the homogenate using a filter membrane with a pore size of 0.45 μm to obtain a first mixture, which includes Rhodiola rosea cell extract, ginseng cell extract, snow lotus cell extract, and safflower cell extract; the organic solvent is ethanol; Step (2): Add the tranexamic acid, nicotinamide, water-soluble vitamin E and sodium metabisulfite to an emulsifying pot and mix and stir. Add an appropriate amount of water and heat to 35-45°C under continuous stirring to fully dissolve them and obtain the second mixture. Step (3): Place the first mixture and the second mixture into the emulsification pot, heat to 70-80°C under continuous stirring, and homogenize them together; stir and cool down to 30-40°C, let it age, and prepare the whitening and anti-aging composition.

2. The whitening and anti-aging composition according to claim 1, characterized in that, The amount of sodium metabisulfite is 0.05-0.15 parts.

3. The whitening and anti-aging composition according to claim 1, characterized in that, The proportions of the following components are as follows: Rhodiola rosea cell extract (7.5-8.5 parts), ginseng cell extract (7.5-8.5 parts), snow lotus cell extract (6.5-7.5 parts), safflower cell extract (5.5-6.5 parts), tranexamic acid (3.5-4.5 parts), nicotinamide (0.45-0.55 parts), water-soluble vitamin E (3.5-4.5 parts), disodium EDTA (0.1-0.15 parts), and sodium metabisulfite (0.06-0.08 parts).

4. The whitening and anti-aging composition according to claim 1, characterized in that, The heating temperature in steps (2) and (3) is 73-75℃.

5. The whitening and anti-aging composition according to claim 1, characterized in that, In step (1), the weight ratio of the total weight of the cell culture to the weight of the water is 1:5-1:10, and the weight ratio of the cell culture to the weight of the organic solvent is 1:5-1:

10.

6. The whitening and anti-aging composition according to claim 1, characterized in that, The total weight ratio of the cell extract to the disodium EDTA is 10:1 to 20:

1.

7. A skincare product containing the whitening and anti-aging composition according to any one of claims 1 to 6, characterized in that, The skincare products include face cream, eye cream, facial cleanser, face mask, BB cream, serum, sunscreen, toner, and lotion.

8. The use of the composition according to any one of claims 1 to 6 in the preparation of cosmetics for whitening, removing freckles, moisturizing, fading spots, smoothing wrinkles, or delaying aging.

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