A method for enhancing the selenium-rich ability of Polygonatum cyrtonema Hua

Abstract

The present invention discloses a method for enhancing the selenium-rich ability of Polygonatum cyrtonema Hua. The Polygonatum cyrtonema Hua is planted in selenium-rich soil. After one month when the leaves of Polygonatum cyrtonema Hua emerge, a salicylic acid with a concentration of 0.3 mmol / L to 1.5 mmol / L is sprayed on the leaves once every 25 to 40 days until spraying stops before the leaves naturally fall off in autumn. Compared with the prior art, after spraying salicylic acid on the leaves of Polygonatum cyrtonema Hua, its selenium enrichment ability is significantly improved, which can be directly applied to the production and cultivation field of Polygonatum cyrtonema Hua, increase the content of trace element selenium in Polygonatum cyrtonema Hua, generate direct economic benefits, and is suitable for popularization and use.

Description

Technical Field

[0001] The present invention relates to the technical field of Polygonatum cyrtonema Hua cultivation, and specifically to a method for enhancing the selenium-enriching ability of Polygonatum cyrtonema Hua. Background Art

[0002] Selenium is an essential element for humans and animals. Organisms cannot synthesize it by themselves and generally obtain it from food. Research shows that selenium has antioxidant activity and can reduce the risk of various human diseases, including cardiovascular diseases, autoimmune thyroid diseases, type 2 diabetes, viral infections, and cancers. Selenium deficiency can lead to Keshan disease and Kashin-Beck disease. Moreover, inorganic selenium in the natural environment is not easily absorbed and utilized by humans, while organic selenium synthesized by plants is beneficial for human absorption and has fewer side effects. Most regions in China are selenium-deficient to varying degrees, but the selenium content in the natural environment of Enshi, Yichun and other regions is relatively high. Therefore, enhancing the selenium enrichment ability of plants is of great significance.

[0003] Polygonatum cyrtonema Hua Liliaceae belongs to the genus Polygonatum of the Liliaceae family and is a perennial herb. Polygonatum was first recorded in "Shennong Ben Cao Jing" and has a medicinal history of more than 2,000 years and a cultivation history of more than 1,000 years in China. It can be used as food in times of famine. Polygonatum cyrtonema Hua has pharmacological effects such as anti-tumor, antioxidant, anti-aging, anti-fatigue, hypoglycemic and lipid-lowering, anti-atherosclerotic, bactericidal and anti-inflammatory activities. It is one of the best dietary therapy products for diabetic patients and can better improve people's yin-deficient constitution and sub-healthy status.

[0004] Some Polygonatum cyrtonema Hua cultivation methods are disclosed in the prior art as follows:

[0005] 1. A method for seed germination and seedling raising of Polygonatum cyrtonema Hua (Publication No.: CN201510416293.6), which includes steps such as fruit self-sealing bag fermentation treatment, low-temperature stratification treatment, hormone treatment to break seed dormancy and induce dormant seeds to become seedlings. By breaking the dormancy of Polygonatum cyrtonema Hua seeds and seed buds, the seedling raising cycle of Polygonatum cyrtonema Hua can be greatly shortened, production costs can be reduced, the propagation coefficient of Polygonatum cyrtonema Hua can be increased, and the problem of insufficient supply of high-quality seedlings of Polygonatum cyrtonema Hua can be solved.

[0006] 2. An efficient seedling raising method for Polygonatum cyrtonema Hua (Publication No.: CN111512965B), which uses the medicinal remaining parts of the rhizomes of 4-5-year-old healthy and disease-free Polygonatum cyrtonema Hua as explants for asexual propagation to obtain Polygonatum cyrtonema Hua rooting seedlings. Then, the obtained rooting seedlings are dipped in plant ash on the surface and mixed with sand for refrigeration, and then sown in the seedling bed. For rhizome seedling raising of Polygonatum cyrtonema Hua, the emergence time is 18d - 25d, which is 120 - 150d shorter than the conventional tuber seedling raising cycle. Moreover, the emergence is neat, the growth of seedlings is good, and the survival rate is high, which can meet the needs of large-scale seedling raising.

[0007] Polygonatum cyrtonema Hua can be planted in selenium-rich areas such as Yichun, etc. However, the planting methods and techniques of selenium-rich Polygonatum cyrtonema Hua have not been disclosed in the existing technology. Therefore, improving the selenium enrichment ability of Polygonatum cyrtonema Hua in the natural environment will have a significant driving force for the production and sales of Polygonatum cyrtonema Hua and improving human health.

[0008] Therefore, it is necessary to propose a method that can enhance the selenium enrichment ability of Polygonatum cyrtonema Hua. Summary of the Invention

[0009] Aiming at the deficiencies of the existing technology, the present invention provides a method that can enhance the selenium enrichment ability of Polygonatum cyrtonema Hua, which can improve the selenium enrichment ability of Polygonatum cyrtonema Hua in the natural environment.

[0010] To achieve the above purposes, the present invention is realized through the following technical solutions:

[0011] A method for enhancing the selenium enrichment ability of Polygonatum cyrtonema Hua, the method steps are as follows: plant Polygonatum cyrtonema Hua in selenium-rich soil. After one month when the leaves of Polygonatum cyrtonema Hua grow, spray salicylic acid with a concentration of 0.3 mmol / L to 1.5 mmol / L on the leaves every 25 - 40 days until spraying stops before the leaves naturally fall off in autumn.

[0012] Preferably, the planted Polygonatum cyrtonema Hua uses at least two-year-old Polygonatum cyrtonema Hua tubers that have not yet unfurled leaves.

[0013] Preferably, after planting Polygonatum cyrtonema Hua in selenium-rich soil, water it every 6 - 8 days, and pour Hoagland solution 15 days later.

[0014] Preferably, the Hoagland solution is watered at a concentration of 50 ml and 0.5 g / L to 0.7 g / L.

[0015] Preferably, after planting Polygonatum cyrtonema Hua in selenium-rich soil, pour sodium selenite solution every 23 - 27 days.

[0016] Preferably, the sodium selenite solution is watered at a concentration of 50 ml and 5 mg / L to 15 mg / L.

[0017] Preferably, spray salicylic acid on the leaves in a spray manner.

[0018] Preferably, the spraying concentration of salicylic acid is preferably 0.5 mmol / L.

[0019] Advantages of the present invention: After spraying salicylic acid on the leaves of Polygonatum cyrtonema Hua, since salicylic acid is a phenolic plant growth regulator contained in plants, it can be absorbed by the leaves, stems and flowers of plants and has a considerable conduction effect. By using the characteristics of salicylic acid to regulate crop growth and improve crop stress resistance to promote the selenium tolerance of crops, its selenium enrichment ability is significantly improved. It can be directly applied to the production and cultivation field of Polygonatum cyrtonema Hua, increase the content of trace element selenium in Polygonatum cyrtonema Hua, generate direct economic benefits, and is suitable for popularization and use. Specific embodiments

[0020] It should be noted that, without conflict, the embodiments in the present invention and the features in the embodiments can be combined with each other.

[0021] The present invention provides a technical solution: a method for enhancing the selenium enrichment ability of Polygonatum cyrtonema Hua, and the method steps are as follows:

[0022] Plant Polygonatum cyrtonema Hua in selenium-rich soil. After one month when the leaves of Polygonatum cyrtonema Hua emerge, spray the leaves with salicylic acid at a concentration of 0.3 mmol / L to 1.5 mmol / L every 25 - 40 days until spraying stops before the leaves naturally fall off in autumn.

[0023] Among them, the planted Polygonatum cyrtonema Hua is selected from at least two-year-old Polygonatum cyrtonema Hua tubers that have not yet unfurled leaves. After planting Polygonatum cyrtonema Hua in selenium-rich soil, water it every 6 - 8 days, and pour Hoagland solution 15 days later. The Hoagland solution is poured at a concentration of 50 ml and a concentration of 0.5 g / L to 0.7 g / L. After planting Polygonatum cyrtonema Hua in selenium-rich soil, pour sodium selenite solution every 25 days, and the sodium selenite solution is poured at a concentration of 50 ml and a concentration of 5 mg / L to 15 mg / L. In practical applications, salicylic acid is sprayed on the leaves in a spray manner, and the preferred spraying concentration of salicylic acid is 0.5 mmol / L.

[0024] After spraying salicylic acid on the leaves of Polygonatum cyrtonema Hua, since salicylic acid is a phenolic plant growth regulator contained in plants, it can be absorbed by the leaves, stems and flowers of plants and has a considerable conduction effect. By using the characteristics of salicylic acid to regulate crop growth and improve crop stress resistance to promote the selenium tolerance of crops, its selenium enrichment ability is significantly improved. It can be directly applied to the production and cultivation field of Polygonatum cyrtonema Hua, increase the content of trace element selenium in Polygonatum cyrtonema Hua, generate direct economic benefits, and is suitable for popularization and use.

[0025] Example 1: Transplant three-year-old Polygonatum cyrtonema Hua to selenium-rich soil, water it once every 7 days, after 25 days, pour 50 ml of Hoagland solution with a concentration of 0.5 g / L, and pour 50 ml of sodium selenite solution with a concentration of 5 mg / L every 25 days until harvest. One month after the leaves emerge, spray the leaves with salicylic acid with a concentration of 0.3 mmol / L using a sprayer, and spray once every 25 days until the leaves naturally fall off in autumn.

[0026] Example 2: Transplant three-year-old Polygonatum cyrtonema Hua to selenium-rich soil, water it once every 7 days, after 25 days, pour 50 ml of Hoagland solution with a concentration of 0.5 g / L, and pour 50 ml of sodium selenite solution with a concentration of 5 mg / L every 25 days until harvest. One month after the leaves emerge, spray the leaves with salicylic acid with a concentration of 0.5 mmol / L using a sprayer, and spray once every 25 days until the leaves naturally fall off in autumn.

[0027] Comparative Example 1: Transplant three-year-old Polygonatum cyrtonema Hua to selenium-rich soil, water it once every 7 days, after 25 days, pour 50 ml of Hoagland solution, and pour 50 ml of sodium selenite solution every 25 days until harvest. One month after the leaves emerge, spray the leaves with salicylic acid with a concentration of 0.5 mmol / L using a sprayer, and spray once every 40 days until the leaves naturally fall off in autumn.

[0028] Comparative Example 2: Transplant three-year-old Polygonatum cyrtonema Hua to selenium-rich soil, water it once every 10 days, after 25 days, pour 50 ml of Hoagland solution, and pour 50 ml of sodium selenite solution every 25 days until harvest. One month after the leaves emerge, spray the leaves with salicylic acid with a concentration of 1.5 mmol / L using a sprayer, and spray once every 25 days until the leaves naturally fall off in autumn.

[0029] Harvest the whole plant of the above Polygonatum cyrtonema Hua, wash it clean with deionized water, dry it at 60 °C for 48 h, and package the roots, rhizomes, and leaves separately. The total selenium concentration in the samples was determined by the standard methods GB 5009.93-2010 and GB 5009.268-2016 formulated by the Ministry of Health of the People's Republic of China. Briefly, the dried plant samples were digested by microwave in a closed container with nitric acid, and the selenium concentration was determined by inductively coupled plasma mass spectrometry (ICP-MS) to measure the total selenium content of the samples. 20 plants of Polygonatum cyrtonema Hua were measured for each example or comparative example.

[0030] For the Polygonatum cyrtonema Hua obtained by the methods of the above examples and comparative examples, the selenium content of Polygonatum cyrtonema Hua was measured, as shown in Table 1:

[0031] Table 1 Selenium content of Polygonatum cyrtonema Hua in different examples

[0032]

[0033] Comparative analysis 1: Combining Example 1 and Comparative Example 1, it can be seen that the selenium content of Polygonatum cyrtonema in Comparative Example 1 is significantly higher than that in Example 1. Increasing the concentration of salicylic acid sprayed will significantly enhance the selenium enrichment ability of crops.

[0034] Comparative analysis 2: Combining Example 2 and Comparative Example 1, it can be seen that the selenium content of Polygonatum cyrtonema in Example 2 is significantly higher than that in Comparative Example 1. Increasing the interval days of spraying salicylic acid will reduce the selenium content of Polygonatum cyrtonema. A shorter interval days can significantly enhance the selenium enrichment ability.

[0035] Comparative analysis 3: Combining Example 2 and Comparative Example 2, it can be seen that the selenium content of Polygonatum cyrtonema in Comparative Example 2 is significantly lower than that in Example 2, indicating that too high a concentration of salicylic acid sprayed will reduce the selenium enrichment amount of Polygonatum cyrtonema.

[0036] Experimental example 1:

[0037] Fill plastic flowerpots with a diameter of 12 cm with substrate and pour it thoroughly with deionized water. A total of 4 treatment groups are set up, with 20 pots in each group. Transplant the three-year-old Polygonatum cyrtonema tubers that have not yet unfolded leaves into the flowerpots one by one. In the first treatment group, water is poured once every 7 days, and 50 ml of 0.5 g / L Hoagland solution is poured after 15 days. Pour 50 ml of sodium selenite solution (concentration of 5 mg / L) every 25 days until harvest, and a total of 8 times of sodium selenite are irrigated. In the second treatment group, water is poured once every 7 days, and 50 ml of 0.5 g / L Hoagland solution is poured after 15 days. Pour 50 ml of sodium selenite solution (concentration of 5 mg / L) every 25 days until harvest, and a total of 8 times of sodium selenite are irrigated. In April, one month after the leaves of Polygonatum cyrtonema emerge, spray 0.5 mmol / L salicylic acid on the leaves every 25 days until September, and a total of 7 times of salicylic acid are sprayed. In the third treatment group, water is poured once every 7 days, and 50 ml of 0.5 g / L Hoagland solution is poured after 15 days. Pour 50 ml of sodium selenite solution (concentration of 15 mg / L) every 25 days until harvest, and a total of 8 times of sodium selenite are irrigated. In the fourth treatment group, water is poured once every 7 days, and 50 ml of Hoagland solution with a concentration of 0.5 g / L is poured after 15 days. Pour 50 ml of sodium selenite solution (concentration of 15 mg / L) every 25 days until harvest, and a total of 8 times of sodium selenite are irrigated. In April, one month after the leaves of Polygonatum cyrtonema emerge, spray 0.5 mmol / L salicylic acid on the leaves every 25 days until September, and a total of 7 times of salicylic acid are sprayed.

[0038] The above 4 treatment groups were harvested as a whole plant, washed clean with deionized water, dried at 60 °C for 48 h, and the roots, rhizomes and leaves were packaged separately. The total selenium concentration in the samples was determined by the standard methods GB5009.93-2010 and GB 5009.268-2016 formulated by the Ministry of Health of the People's Republic of China. Briefly, the dried plant samples were digested by microwave in a closed container with nitric acid, and the selenium concentration was determined by inductively coupled plasma mass spectrometry (ICP-MS) to measure the total selenium content of the samples; the dried samples were weighed and added to hydrogen chloride, and after mixing, the samples were placed in a constant temperature water bath at 70 °C, shaken for 2 h, filtered with absorbent cotton, boiled in a water bath for 20 min, and potassium ferricyanide was added. The content of inorganic selenium was determined by an atomic fluorescence spectrophotometer. The content of organic selenium in the sample was equal to the total selenium content minus the inorganic selenium content. The results are shown in Table 2:

[0039]

[0040]

[0041] Table 2 Selenium content of Polygonatum cyrtonema Hua under the treatment of salicylic acid and sodium selenite at different concentrations

[0042] Comparative analysis 4: As can be seen from Table 2, compared with the first treatment group and the second treatment group, when the same 8 times of 5 mg / L sodium selenite was irrigated, the total selenium dry weight, organic selenium dry weight and inorganic selenium dry weight in the second treatment group with the spraying of salicylic acid step were significantly higher than those in the first treatment group, indicating that the method of spraying salicylic acid on the leaves of this application can significantly enhance the selenium enrichment ability of crops. Compared with the first treatment group and the third treatment group, it shows that without spraying salicylic acid, when the concentration of sodium selenite irrigated is increased from 5 mg / L to 15 mg / L, the selenium enrichment amount of crops can be increased. Combining the second treatment group and the fourth treatment group, it shows that under the irrigation of different concentrations of sodium selenite, spraying salicylic acid treatment can effectively enhance the selenium enrichment ability of Polygonatum cyrtonema Hua, and has extremely high popularization and application value.

[0043] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A method for enhancing the selenium-enriching ability of Polygonatum cyrtonema Hua, characterized in that, Including the following steps: Plant Polygonatum cyrtonema Hua in selenium-rich soil, water it every 6 - 8 days, pour Hoagland solution after 15 days, pour sodium selenite solution every 23 - 27 days. When one month has passed since the leaves of Polygonatum cyrtonema Hua emerged, spray salicylic acid with a concentration of 0.3 mmol / L - 1.5 mmol / L on the leaves every 25 - 40 days until spraying stops before the leaves naturally fall off in autumn.

2. The method for enhancing the selenium-rich ability of Polygonatum cyrtonema Hua according to claim 1, wherein The planted Polygonatum cyrtonema Hua uses at least two-year-old rhizomes of Polygonatum cyrtonema Hua that have not yet unfurled leaves.

3. The method for enhancing the selenium-rich ability of Polygonatum cyrtonema Hua according to claim 1, characterized in that The Hoagland solution is watered at a concentration of 50 ml and 0.5 g / L - 0.7 g / L.

4. The method for enhancing the selenium-rich ability of Polygonatum cyrtonema Hua according to claim 1, characterized in that The sodium selenite solution is watered at a concentration of 50 ml and 5 mg / L - 15 mg / L.

5. The method for enhancing the selenium enrichment ability of Polygonatum cyrtonema Hua as described in claim 1, characterized in that Salicylic acid is sprayed on the leaves in a spray manner.

6. The method for enhancing the selenium enrichment ability of Polygonatum cyrtonema Hua as described in claim 1, wherein The spraying concentration of salicylic acid is 0.5 mmol / L.

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