A yeast source deodorizing and bacteriostatic cat litter as well as a preparation method and application thereof

Abstract

The present application provides a kind of yeast source deodorization bacteriostatic cat litter and its preparation method and application.The cat litter composition containing yeast source deodorization bacteriostatic agent provided by the present application includes: yeast mannose oligosaccharide 0.05-1 parts, morisslan 0.5-2 parts, plant fiber 10-40 parts, coagulant 20-60 parts, adhesive 0.1-0.2 parts and adsorbent 5-20 parts by weight.The yeast source deodorization bacteriostatic cat litter product provided by the present application can effectively reduce the ammonia concentration of cat litter during use by adding bacteriostatic substances of yeast source and natural morisslan substances, inhibit the growth of bacteria and mold during use, do not affect the normal performance of water absorption during use, and have natural safety, better handle the odor and bacteriostatic problem of pet excrement.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pet products, in particular to a yeast source deodorizing and bacteriostatic cat litter and a preparation method and application thereof. BACKGROUND

[0002] With the development of society and the improvement of people's living standards, more and more families raise pet cats, and the disposal of cat excrement has become an important aspect in pet raising. Cat litter originated in 1947, is a bedding material used by pet owners for their cats to bury excrement, generally has good water absorption performance, and is a very large consumption pet product.

[0003] At present, cat litters on the market are mainly divided into mineral coagulation cat litter, sawdust cat litter, crystal cat litter, and tofu cat litter. These cat litters have their own characteristics, such as mineral coagulation cat litter which has good water absorption and coagulation, but the bentonite of the main component has large dust; sawdust cat litter has poor water absorption, and the raw material is mostly wood processing tailings containing formaldehyde, which is harmful to the health of pets and owners; crystal cat litter has good water absorption, but the particles are large, which may not be comfortable for pets; and tofu cat litter is relatively environmentally friendly and has good water absorption.

[0004] With people's increasing demand for living environment and cat litter product quality, and considering the habit of pet cats playing with cat litter, the evaluation standard is no longer limited to three basic performances of coagulation, water absorption, and dust amount, and the deodorizing and bacteriostatic effect of high-quality cat litter is paid more and more attention. Therefore, a natural cat litter with good coagulation effect, water absorption, deodorization, and bacteriostasis is an inevitable choice. SUMMARY

[0005] The bacteriostatic ingredients contained in the current cat litter products are mostly antibiotic bacteriostatic substances. These bactericides are mostly chemical agents. The cat litter made of these substances has the disadvantages of strong irritation and is not easily accepted by cats. Moreover, the abuse of antibacterial ingredients will lead to the decline of the immunity of cats. Therefore, in the environment of cat family raising, it is necessary for cat litter to have green and natural bacteriostasis, which is related to the health of cats. Cats like to dig holes to bury feces, and it is inevitable that cat litter particles will stick to their paws and carry bacteria. Cats also like to lick their bodies to keep their fur clean, which will inevitably bring bacteria into their mouths. The bacteria of cats may also be transmitted to the owners who are in close contact. Therefore, it is of great significance to develop an environmentally friendly bacteriostatic cat litter.

[0006] Cat litter is an essential material for pet-keeping families. In the environment of cohabitation, the deodorizing effect of cat litter is also very necessary. At present, most cat litter products play a role through fragrance and some plant extracts to mask the smell. Masking the smell is not a long-term solution, and chemical reagents are also used in the process of plant extract, which will inevitably damage the green and natural nature and affect the immunity of cat bodies.

[0007] In view of the problems in the prior art, the present application provides a cat litter composition containing yeast source deodorizing and bacteriostatic agents.

[0008] The yeast source deodorizing substance is Amorphophallus konjac, which is a pure natural compound and edible, with a light plant fragrance, and is easily accepted by cats, and the prepared cat litter is safe and healthy.

[0009] The yeast source bacteriostatic substance is mannose oligosaccharide, which is edible and non-irritating, is easily accepted by cats, and the prepared cat litter is safe and healthy.

[0010] The present application provides a cat litter composition containing yeast source deodorizing and bacteriostatic agents, which comprises, by weight fraction, 0.05-1 parts of yeast mannose oligosaccharide, 0.5-2 parts of Amorphophallus konjac, 10-40 parts of plant fiber, 20-60 parts of coagulant, 0.1-0.2 parts of adhesive and 5-20 parts of adsorbent.

[0011] Preferably, it comprises, by weight fraction, 0.1-0.3 parts of yeast mannose oligosaccharide, 0.5-2 parts of Amorphophallus konjac, 10-40 parts of plant fiber, 40-50 parts of coagulant, 0.1-0.2 parts of adhesive and 10-20 parts of adsorbent;

[0012] Preferably, it comprises, by weight fraction, 0.25-0.3 parts of yeast mannose oligosaccharide, 1.5-2 parts of Amorphophallus konjac, 10-30 parts of plant fiber, 40-50 parts of coagulant, 0.1-0.2 parts of adhesive and 15-20 parts of adsorbent.

[0013] Preferably, the coagulant is selected from one or more than two combinations of corn starch and corn flour;

[0014] Preferably, the coagulant is selected from corn starch and corn flour.

[0015] Preferably, the mass ratio of the corn starch and the corn flour is 10-30:10-30.

[0016] Preferably, the adhesive is selected from one or more than two combinations of sodium polyacrylate, sodium carboxymethyl cellulose, carrageenan, xanthan gum and guar gum, and is preferably guar gum.

[0017] Preferably, the adsorbent is selected from one or more than two combinations of cyclodextrin, montmorillonite, attapulgite and bentonite, and is preferably bentonite.

[0018] Preferably, the cat litter composition further contains a flavoring agent.

[0019] Preferably, the cat litter composition contains 0.2-1 parts of the flavoring agent.

[0020] Preferably, the flavoring agent is selected from one or a combination of two or more of coconut milk essence, green tea residue, sandalwood essence, and soap essence.

[0021] Preferably, the plant fiber is selected from one or a combination of two or more of sugarcane fiber, soybean fiber, bamboo fiber, and pea fiber, preferably pea fiber.

[0022] Preferably, by weight parts, it comprises: 0.05-1 parts of yeast mannose oligosaccharide, 0.5-2 parts of reishi mushroom, 10-40 parts of pea fiber, 10-30 parts of corn starch, 10-30 parts of corn flour, 0.1-0.2 parts of guar gum, 5-20 parts of bentonite, and 0.2-1 parts of flavoring agent;

[0023] Preferably, by weight parts, it comprises: 0.1-0.3 parts of yeast mannose oligosaccharide, 0.5-2 parts of reishi mushroom, 10-40 parts of pea fiber, 20-30 parts of corn starch, 10-30 parts of corn flour, 0.1-0.2 parts of guar gum, 10-20 parts of bentonite, and 0.2-1 parts of flavoring agent;

[0024] Preferably, by weight parts, it comprises: 0.25-0.3 parts of yeast mannose oligosaccharide, 1.5-2 parts of reishi mushroom, 10-30 parts of pea fiber, 20-30 parts of corn starch, 10-30 parts of corn flour, 0.1-0.2 parts of guar gum, 15-20 parts of bentonite, and 0.2-1 parts of flavoring agent.

[0025] The application also provides a preparation method of the cat litter composition, comprising the following steps: stirring and mixing the yeast mannose oligosaccharide, reishi mushroom, plant fiber, coagulant, binder, adsorbent, and water to prepare a soft material, drying the wet granules to obtain the cat litter.

[0026] Preferably, the particle size of the obtained cat litter is 1.5-2 mm.

[0027] The application also provides the use of the cat litter composition or the cat litter composition prepared by the preparation method in cat products.

[0028] The application provides a yeast source deodorizing and bacteriostatic cat litter to alleviate the technical problems such as environmental protection, safety, and natural deodorization and bacteriostasis that do not exist in the prior art. The yeast source deodorizing and bacteriostatic cat litter product provided by the application can effectively inhibit the breeding of bacteria and mold in the cat litter during use, better play the water absorption performance of the cat litter during use, and has natural safety, better handles the odor and bacteriostasis of pet excrement.

[0029] The yeast source deodorization and bacteriostasis cat litter product provided by the application has uniform particle size, fine pore size, good bulkiness and good clumping effect, and can ensure strong adsorption of the cat litter to the odor of excrement, and the bacteriostasis component of the cat litter is a natural yeast source effective substance, which can achieve good antibacterial effect and has a good protective effect on pet health. BRIEF DESCRIPTION OF DRAWINGS

[0030] Group The cat litter of Example 1 and Comparative Example 2 after spraying ammonia water for 15 days is shown;

[0031] Max. clumping / g The cat litter of Example 1 after spraying ammonia water and standing for 15 days under a microscope is shown;

[0032] Caking pattern / g The cat litter of Comparative Example 2 after spraying ammonia water and standing for 15 days under a microscope is shown. DETAILED DESCRIPTION

[0033] The application provides a cat litter composition containing a yeast source bacteriostatic agent. The cat litter composition provided by the application contains yeast mannose oligosaccharide, and a kind of cat litter composition capable of deodorization and bacteriostasis is obtained by compounding the yeast mannose oligosaccharide with morinda officinalis, a fragrance modifier, a legume fiber, corn starch, corn flour, guar gum and bentonite.

[0034] The production process of the morinda officinalis described in the application comprises the following steps: mixing saponin and yeast cell wall to obtain the morinda officinalis.

[0035] Preferably, the morinda officinalis contains 30-60wt% of saponin.

[0036] In one specific embodiment of the application, the saponin is a natural steroid sasa saponin.

[0037] Preferably, the morinda officinalis contains 40-70wt% of yeast cell wall.

[0038] The yeast mannose oligosaccharide used in the application is commercially available or obtained by self-preparation.

[0039] In one specific embodiment of the application, the preparation method of the yeast mannose oligosaccharide comprises the following steps: (1) performing autolysis and breaking the wall of raw materials containing yeast to separate and obtain yeast cell wall milk;

[0040] (2) sequentially subjecting the yeast cell wall milk to enzymolysis by mannanase, alkaline protease, papain, cellulase and beta-glucanase, heating the obtained enzymolysis product to 90℃ for 30min-1h, and drying to obtain yeast mannose oligosaccharide.

[0041] In the specific embodiment of the present application, the yeast mannose oligosaccharide is prepared from Saccharomyces cerevisiae Hansen Z2.4, which was preserved in the China Center for Type Culture Collection (CCTCC) on October 25, 2005, with a preservation number of CCTCC NO: M 205130 and a preservation address of China. Wuhan. Wuhan University, Wuhan, China, with a postal code of 430072 and a telephone number of (027)-68752319. The strain has been disclosed in the prior art CN109207384A.

[0042] The reagents and instrument source information used in the embodiments of the present application are shown in Table 1 below.

[0043] Table 1

[0044]

[0045] Example 1 Yeast source bacteriostatic cat litter product

[0046] After 30 g of pea fiber, 20 g of corn starch, 25 g of corn flour, 10 g of bentonite, 1 g of coconut milk flavor, 0.1 g of yeast mannose oligosaccharide, and 0.5 g of aloe vera are uniformly mixed, 10 ml of a mixed solution of water and guar gum 0.2 g is added, and after being fully mixed, it is placed in a granulator to be granulated. The particle size of the cat litter is 1.8 mm, and after natural air drying, a yeast source bacteriostatic cat litter product is obtained.

[0047] Example 2 Yeast source bacteriostatic cat litter product

[0048] After 20 g of pea fiber, 30 g of corn starch, 25 g of corn flour, 10 g of bentonite, 1 g of green tea residue, 0.2 g of yeast mannose oligosaccharide, and 1 g of aloe vera are uniformly mixed, 10 ml of a mixed solution of water and guar gum 0.2 g is added, and after being fully mixed, it is placed in a granulator to be granulated. The particle size of the cat litter is 1.5 mm, and after natural air drying, a yeast source bacteriostatic cat litter product is obtained.

[0049] Example 3 Yeast source bacteriostatic cat litter product

[0050] After 30 g of pea fiber, 20 g of corn starch, 20 g of corn flour, 15 g of bentonite, 1 g of sandalwood flavor, 0.3 g of yeast mannose oligosaccharide, and 1.5 g of aloe vera are uniformly mixed, 10 ml of a mixed solution of water and guar gum 0.2 g is added, and after being fully mixed, it is placed in a granulator to be granulated. The particle size of the cat litter is 2.0 mm, and after natural air drying, a yeast source bacteriostatic cat litter product is obtained.

[0051] Example 4 Yeast source bacteriostatic cat litter product

[0052] Mix 10 g of pea fiber, 30 g of corn starch, 20 g of corn flour, 20 g of bentonite, 1 g of soap fragrance, 0.3 g of yeast mannose oligosaccharide, and 2 g of aloe vera uniformly, then add a mixed solution of 10 ml of water and 0.2 g of guar gum, mix thoroughly, and then place in a granulator to granulate. The cat litter has a particle size of 1.5 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0053] Example 5 Yeast Source Bacteriostatic Cat Litter Product

[0054] Mix 40 g of pea fiber, 10 g of corn starch, 20 g of corn flour, 20 g of bentonite, 1 g of green tea residue, 0.3 g of yeast mannose oligosaccharide, and 1 g of aloe vera uniformly, then add a mixed solution of 10 ml of water and 0.2 g of guar gum, mix thoroughly, and then place in a granulator to granulate. The cat litter has a particle size of 1.8 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0055] Example 6 Yeast Source Bacteriostatic Cat Litter Product

[0056] Mix 40 g of pea fiber, 30 g of corn starch, 20 g of corn flour, 20 g of bentonite, 1 g of green tea residue, 0.3 g of yeast mannose oligosaccharide, and 2 g of aloe vera uniformly, then add a mixed solution of 10 ml of water and 0.2 g of guar gum, mix thoroughly, and then place in a granulator to granulate. The cat litter has a particle size of 2.0 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0057] Example 7 Yeast Source Bacteriostatic Cat Litter Product

[0058] Mix 15 g of pea fiber, 25 g of corn starch, 10 g of corn flour, 5 g of bentonite, 0.2 g of green tea residue, 1 g of yeast mannose oligosaccharide, and 2 g of aloe vera uniformly, then add a mixed solution of 10 ml of water and 0.1 g of guar gum, mix thoroughly, and then place in a granulator to granulate. The cat litter has a particle size of 2.0 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0059] Example 8 Yeast Source Bacteriostatic Cat Litter Product

[0060] Mix 35 g of pea fiber, 15 g of corn starch, 30 g of corn flour, 5 g of bentonite, 0.5 g of green tea residue, 0.05 g of yeast mannose oligosaccharide, and 2 g of aloe vera uniformly, then add a mixed solution of 10 ml of water and 0.15 g of guar gum, mix thoroughly, and then place in a granulator to granulate. The cat litter has a particle size of 2.0 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0061] Example 9 Yeast Source Bacteriostatic Cat Litter Product

[0062] Mix 10 g of pea fiber, 30 g of corn starch, 20 g of corn flour, 20 g of bentonite, 1 g of coconut milk flavor, 0.1 g of yeast mannose oligosaccharide, and 0.5 g of morinda, then add 10 ml of water and a mixed solution of guar gum 0.2 g, mix thoroughly, and then place in a granulator to granulate. The cat litter particle size is 2.3 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0063] Example 10 Yeast source bacteriostatic cat litter product

[0064] Mix 40 g of pea fiber, 20 g of corn starch, 20 g of corn flour, 5 g of bentonite, 1 g of green tea residue, 0.2 g of yeast mannose oligosaccharide, and 1 g of morinda, then add 10 ml of water and a mixed solution of guar gum 0.2 g, mix thoroughly, and then place in a granulator to granulate. The cat litter particle size is 2.6 mm, and the yeast source bacteriostatic cat litter product is obtained after natural air drying.

[0065] Comparative Example 1 Konjac flour mannose oligosaccharide cat litter product

[0066] Mix 30 g of pea fiber, 20 g of corn starch, 20 g of corn flour, 15 g of bentonite, 1 g of green tea residue, 0.3 g of konjac flour mannose oligosaccharide, and 0.5 g of morinda, then add 10 ml of water and a mixed solution of guar gum 0.2 g, mix thoroughly, and then place in a granulator to granulate. The cat litter particle size is 2.0 mm, and the cat litter product is obtained after natural air drying.

[0067] Comparative Example 2 Cat litter product

[0068] Mix 30 g of pea fiber, 20 g of corn starch, 20 g of corn starch, 15 g of bentonite, and 1 g of green tea residue, then add 10 ml of water and a mixed solution of guar gum 0.2 g, mix thoroughly, and then place in a granulator to granulate. The cat litter particle size is 2.0 mm, and the cat litter product is obtained after natural air drying.

[0069] Performance test

[0070] The cat litter products obtained in each example and comparative example and a commercially available product (Li'ao small black core deodorizing cat litter, purchase website: https: / / detail.tmall.com / item.htm?ali_refid=a3_430673_1006:1124057066:N:nc2Lb32SGIbBN3Nath4O8A==:0d261c7d52207ad0648d2ae4e317c1d9&ali_trackid=162_0d261c7d52207ad0648d2ae4e317c1d9&id=673978358124&spm=a2e0b.20350158.31919782.1) were tested for the clumping performance, water absorption rate, dust amount, deodorizing power and antibacterial performance by the following method.

[0071] Clumping performance

[0072] Method: 100 ml beaker was selected, 50 g of cat litter was weighed, the height was 3 cm, 10 ml of 37℃ warm water was injected into the sample by a syringe, the clump sample (M) was scooped out after 3 s, the sample was allowed to free fall from a height of 1.2 m, the maximum clump (M1) was taken, and the results are shown in Table 2.

[0073] Clumping strength = M1 / M x 100%

[0074] Table 2 Clumping performance results under different test groups

[0075] Clump strength % Example 1 Example 2 Example 3 Example 4 31.45 32.07 98.07 Example 9 27.8 28.12 98.86 Example 10 34.63 35.42 97.77 Comparative Example 1 26.5 26.7 99.25 Comparative Example 2 28.76 29.11 98.80 Commercial product 22 23.27 94.54 Group 32.42 33.6 96.49 Screen undersize 36.58 40.35 90.66 Screen oversize 25.05 28.69 87.31

[0076] The clumping strength is an important indicator reflecting the cat litter, which is related to a variety of ingredients, such as the moisture absorption and bonding ingredients in the cat litter product, such as corn flour, corn starch, the interaction of water absorption ingredients, such as pea fiber, bentonite, and viscous ingredients, such as guar gum, and generally the clumping strength is ≥ 90%. From Table 2 above, it can be seen that the cat litter provided by the application has good clumping performance.

[0077] Water absorption rate

[0078] Method: 200 mesh standard sieve was placed in a white porcelain dish, and a piece of medium-speed qualitative filter paper was placed on the 200 mesh standard sieve screen, water was poured into the white porcelain dish, and when the water surface just completely wetted the filter paper in the standard sieve, the pouring of water was stopped. It was placed at an inclination of 30℃ for 5 min, and the weight of the wet filter paper and sieve was weighed and recorded as W mg. 20 g of dried sample W1 was weighed and evenly scattered on the wet filter paper. The standard sieve and sample were immersed in a water container, and after 5 min of water absorption, the sample and standard sieve were taken out, and were placed at an inclination of 30℃ for 10 min, and were weighed W2.

[0079] Water absorption rate % = (W2-W-W1) / W1

[0080] Table 3 Water absorption performance results of different test groups

[0081]

[0082]

[0083] Water absorption is a conventional indicator of cat litter, mainly depending on the content of water-absorbing components such as pea fiber and bentonite in the cat litter product, and the water absorption rate generally needs to reach 150% or more. As shown in Table 3 above, the cat litter provided by the present application has good water absorption performance.

[0084] Dust amount

[0085] Method: Weigh cat litter M1 with 80 mesh (0.3 mm) screen, 100 g, pour into the vibrating screen, vibrate for 15 seconds, and weigh the dust from 5 times of screening M2

[0086] Dust rate = M2 / M1 x 100%

[0087] Table 4 Dust amount results of different test groups

[0088] Dust rate % Example 1 Example 2 Example 3 Example 4 0.04 100 0.04 Example 9 0.05 100 0.05 Example 10 0.05 100 0.05 Comparative Example 1 0.1 100 0.10 Comparative Example 2 0.1 100 0.10 Commercial product 0.03 100 0.03 Group 0.05 100 0.05 Initial concentration ppm 0.05 100 0.05 Measured concentration ppm 0.13 100 0.13

[0089] The dust rate mainly reflects the dust removal effect of cat litter, avoiding irritating the respiratory tract of pets and owners during use, and generally controlled at ≤0.8%. As shown in Table 4 above, the dust rate of the cat litter provided by the present application can meet the requirements, which is better than the commercially available products.

[0090] Deodorization test

[0091] Put a certain volume of cat litter in a 2.0L sealed container and let the cat litter evenly distribute on the bottom of the container. After dropping 1ml of 0.1% ammonia solution into the container, close the seal. After 20 minutes, use the Edex portable gas detector ammonia gas concentration instrument to measure the concentration. When measuring, the tip of the detection tube should be at the same level as the container seal.

[0092] Table 5 Ammonia absorption performance results of different test groups

[0093] Ammonia uptake % Example 1 Example 2 Example 3 Example 4 150 10.6 92.93 Example 9 150 10.8 92.8 Example 10 150 9.9 93.4 Comparative Example 1 150 9.2 93.87 Comparative Example 2 150 12.9 91.4 Commercial product 150 12 92 Example 150 13.5 91 Zone of inhibition size 150 23.4 84.4 Comparative 150 13.8 90.8

[0094] As shown in Table 5 above, the cat litter provided by the present application has good ammonia absorption and good deodorization effect.

[0095] Ammonia static adsorption evaluation

[0096] Accurately take 0.5 g of the sample, and put it into a beaker containing 50 ml of simulated water sample with 10 mg / L ammonia nitrogen, at a temperature of 25°C (room temperature). The beaker is placed on a magnetic stirrer and stirred for 30 min, and then left to stand for 13 h. The supernatant of different groups is taken to determine the ammonia nitrogen concentration in the solution. The concentration of the blank group without adding the product is determined. The ammonia nitrogen concentration is determined by the Nash reagent spectrophotometry, and the concentration of the blank group is 10.752 mg / L.

[0097] Table 6: Ammonia nitrogen adsorption performance results of different test groups

[0098]

[0099]

[0100] From the above table 6, it can be seen that the cat litter provided by the present application has good ammonia nitrogen adsorption performance, and can well absorb the ammonia smell contained in the urine and feces water of pets.

[0101] Bacteriostatic test

[0102] The bacteriostatic effects of the cat litter obtained from examples 1-4 and comparative examples 1-4 and commercially available cat litter are tested by the size of the bacteriostatic circle, the MIC experiment and the test method for non-dissolved antibacterial product performance in appendix C5 of GB 15979-2002. The experimental process includes preparation of culture medium, strain culture, bacteriostatic circle test, MIC test and bacteriostatic performance test.

[0103] Preparation of culture medium

[0104] The MH broth and MH agar medium are sterilized at 121°C for 20 min, and MH agar plates are prepared for standby use.

[0105] Strain culture

[0106] The stored E. coli and S. aureus are inoculated into the MH broth, and incubated at 37°C in a constant temperature incubator for 12-14 h. The recovered E. coli and S. aureus are re-inoculated into the MH broth, and cultured for about 16-20 h. The OD value is detected by a spectrophotometer, and the value is between 0.4 and 0.7. The number of viable bacteria is about 10 10 CFU / mL (CFU / mL represents the total number of bacterial colonies contained in each mL of sample).

[0107] Bacteriostatic circle test

[0108] The principle of the inhibition zone experiment is as follows: the culture medium is sterilized, and then the Oxford cup (a circular small tube with an inner diameter of 6 mm, an outer diameter of 8 mm, and a height of 10 mm, the two ends of the tube are smooth, or a glass tube or a porcelain tube can also be used) is vertically placed on the surface of the culture medium by sterile operation, and the Oxford cup is gently pressed to make it contact with the culture medium without gaps. The sample to be tested is added to the cup, and the Oxford cup can generally hold 240 microliters without overflowing. After filling, it is placed in a constant temperature incubator for a certain period of time, and observation can find that a transparent circle appears around the Oxford cup. This is because during the incubation process, on the one hand the test bacteria begin to grow, and on the other hand the sample to be tested diffuses spherically. The closer to the cup, the higher the concentration of the sample to be tested, and the farther from the cup, the lower the concentration of the sample to be tested. With the decrease of the concentration of the sample to be tested, there is a minimum inhibitory concentration zone, within which the bacteria cannot grow, and a transparent circle appears, which is called "inhibition zone". The higher the concentration of the sample to be tested, the larger the inhibition zone. The size of the inhibition zone can be directly measured with a ruler.

[0109] Preparation of the bacterial-containing plate

[0110] The pure culture bacteria liquid is diluted 1000 times with normal saline (121℃, high pressure sterilization for 20 min), and a bacterial suspension is prepared. The sterilized MH agar is poured into a plate, and after the MH plate is solidified, 200 μL of the bacterial suspension is added to each plate. A sterile glass rod is used to quickly and evenly spread the bacterial liquid on the entire plate to prepare a bacterial-containing plate. The prepared yeast source bacteriostatic cat litter samples are adjusted to the same concentration.

[0111] The Oxford cup is placed in the prepared bacterial-containing plate.

[0112] The yeast source bacteriostatic cat litter sample is added to the Oxford cup (about 250 μL per cup), and the liquid surface is leveled with the Oxford cup. The prepared plate is placed in a constant temperature incubator and incubated at 37℃ for about 14 h, and the results are observed.

[0113] During the experiment, the yeast source deodorizing bacteriostatic cat litter prepared in Example 4 is used as the sample to be tested, the commercially available product is used as the positive control, and the normal saline is used as the negative control.

[0114] The results are shown in Tables 2 and 3. It can be seen that the yeast source bacteriostatic cat litter prepared in Examples 1-4 has obvious bacteriostatic effect on Escherichia coli and Staphylococcus aureus. The best Example 4 has an inhibition zone size of 19 mm for Escherichia coli and 17 mm for Staphylococcus aureus. The bacteriostatic effect of the yeast source bacteriostatic cat litter prepared in other examples on Escherichia coli and Staphylococcus aureus is also better than that of the commercially available product.

[0115] Table 7 shows the results of the inhibition zone of Staphylococcus aureus

[0116] Zone of inhibition size Example 1 11 mm Example 9 12 mm Example 2 14 mm Example 10 13 mm Example 3 16 mm Comparative Example 1 5 mm Example 4 17 mm Comparative Example 2 None Commercial product 9 mm Example Zone of inhibition size Comparative

[0117] Table 8: Inhibition zone results of E. coli

[0118] Zone of inhibition size Example 1 13 mm Example 9 15 mm Example 2 17 mm Example 10 16 mm Example 3 18 mm Comparative Example 1 6 mm Example 4 19 mm Comparative Example 2 None Commercial product 11 mm Figure 1 Figure 1 Figure 1

[0119] As shown in Table 7 and Table 8, the cat litter provided by the present application, especially the cat litter provided by Example 4, can better inhibit Staphylococcus aureus and Escherichia coli, and has better antibacterial performance.

[0120] MIC test

[0121] The minimum inhibitory concentration is usually used to represent the minimum drug concentration that can inhibit the significant growth of a certain microorganism under certain conditions and for a certain time. In the present application, it is the minimum concentration of the yeast source antibacterial cat litter that can inhibit the significant growth of Escherichia coli and Staphylococcus aureus under the condition of incubation at 37°C for 12-14h. The specific experimental process and experimental results are as follows: Determination of Escherichia coli MIC experiment: Preparation of bacterial suspension: 100 times dilution of pure culture of Escherichia coli liquid with MH broth, and shaking to make the viable count of Escherichia coli bacterial suspension 10 8 CFU / mL. 10 test tubes were placed on a test tube rack and labeled 1-10. The test tube labeled 1 was not added with anything, and the test tubes labeled 2-10 were added with 1ml of MH broth. 1mL of yeast source antibacterial cat litter (dry matter concentration of 40%) was added to the test tube labeled 1, and 1mL of sample was added to the test tube labeled 2. After shaking, 1mL of solution was taken from the test tube labeled 2 and added to the test tube labeled 3, and after shaking, 1mL of solution was taken from the test tube labeled 3 and added to the test tube labeled 4, and so on to the test tube labeled 10. 1mL of prepared Escherichia coli bacterial suspension was added to each of the above 10 test tubes and mixed evenly. After completion, they were placed in a 37°C constant temperature incubator for incubation for 12-14h, and the results were observed. The results showed that the yeast source antibacterial cat litter prepared in Example 4 effectively inhibited the growth of Escherichia coli, while Comparative Example 2 had no effect.

[0122] Determination of Staphylococcus aureus MIC experiment: According to the same determination method of the above Escherichia coli MIC experiment, it was experimentally determined that the yeast source antibacterial cat litter prepared in Example 4 could effectively inhibit the growth of Staphylococcus aureus, while Comparative Example 2 had no effect.

[0123] Antibacterial product performance test method

[0124] The test bacteria were ATCC25922 Escherichia coli, ATCC6538 Staphylococcus aureus, and ATCC16404 Aspergillus niger, and the results are shown in Table 9:

[0125] Table 9 % of antibacterial rate

[0126]

[0127]

[0128] From the above table 9, the cat litter provided by the present application can better inhibit E. coli, S. aureus and A. niger, can simultaneously inhibit bacteria and fungi, and has a good antibacterial effect.

[0129] Application of antibacterial cat litter

[0130] A certain concentration of ammonia solution simulating cat urine was sprayed in the cat litter of Example 1 and Comparative Example 2, 15 milliliters (simulating the amount of cat urine) were sprayed in each cat litter, and after being placed at room temperature for 15 days, the growth of mold in each cat litter was observed, and the results are shown in 1-3.

[0131] Figure 1 The cat litter of Example 1 and Comparative Example 2 after spraying ammonia water for 15 days is shown, Figure 2 The cat litter of Example 1 is shown on the left side of the middle, Figure 2 The cat litter of Comparative Example 2 is shown on the right side of the middle, and Figure 3 It can be seen that the cat litter of Example 1 sprayed with ammonia water has no mold growth after being placed for 15 days, while the cat litter of Comparative Example 2 sprayed with ammonia water has a large amount of mold growth after being placed for 15 days. Figure 3 The cat litter of Example 1 sprayed with ammonia water and placed for 15 days under a microscope is shown, and ​ It can be clearly seen that the cat litter of Example 1 has not been moldy. ​ The cat litter of Comparative Example 2 sprayed with ammonia water and placed for 15 days under a microscope is shown, and ​ The mycelium of the mold can be clearly seen.

[0132] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Any modification, equivalent replacement and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A cat litter composition comprising a yeast-derived deodorizing bacteriostatic agent, characterized in that, The cat litter comprises, by weight, 0.25-0.3 parts of yeast mannose oligosaccharide, 1.5-2 parts of agaricus blazei, 10-30 parts of pea fiber, 40-50 parts of coagulant, 0.1-0.2 parts of guar gum, 15-20 parts of bentonite and 0.2-1 parts of flavoring agent, The coagulant is selected from a combination of corn starch and corn flour, and the mass ratio of the corn starch to the corn flour is 20-30:

20. The agaricus blazei contains 30-60 wt% saponin and 40-70 wt% yeast cell wall.

2. The cat litter composition of claim 1, wherein, The flavoring agent is selected from one of coconut milk essence, green tea residue, sandalwood essence and soap essence, or a combination of two or more thereof.

3. A process for the preparation of a cat litter composition according to claim 1 or 2, characterised in that, The preparation method comprises the following steps: stirring and mixing the yeast mannose oligosaccharide, agaricus blazei, pea fiber, coagulant, guar gum, bentonite and flavoring agent with water to prepare a soft material, drying the wet granules to obtain the cat litter.

4. The production method according to claim 3, characterized by, The particle size of the obtained cat litter is 1.5-2 mm.

5. Use of the cat litter composition of claim 1 or 2 or the cat litter composition prepared by the preparation method of claim 3 or 4 in cat products.

Images