Insecticide containing a gemini surfactant and use

Abstract

This invention discloses an insecticide containing a gemini surfactant and its application, belonging to the field of insecticide technology. The insecticide comprises: 0.5-1‰ gemini surfactant, 0.3-0.5‰ synergist, 0-0.3‰ excipients, and the balance being water. The gemini surfactant contained in this insecticide has strong wetting and penetrating properties, eliminating the need for additional wetting and penetrating agents in the insecticide formulation. Furthermore, the small amount of gemini surfactant used simplifies the formulation and reduces the cost of the insecticide while maintaining the same insecticidal effect. The gemini surfactant can quickly penetrate into the body of mosquitoes and flies, synergistically sealing the spiracles of mosquitoes and flies, causing them to suffocate and die, in conjunction with the fatty acid ricinoleic acid.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of insecticides, in particular to an insecticide containing gemini surfactants and application thereof. BACKGROUND

[0002] Mosquitoes and flies are important transmitters of zoonoses, and also have a huge impact on the livestock industry. Mosquitoes and flies can carry more than 65 kinds of zoonoses, and also disturb the peace of livestock and poultry, leading to restlessness, reduced lying time, wasted feed, and reduced milk, meat and egg production. Existing killing methods mainly include physical killing and insecticide poisoning. Insecticide poisoning generally uses pyrethroid insecticides or insect growth regulators such as S-olefin insecticides. Pyrethroid insecticides are highly toxic to fish, shrimp, crab and other aquatic organisms, and have a great harm to the environment. The insecticidal principle of insect growth regulators such as S-olefin insecticides is to interfere with the growth of mosquitoes, so that the larvae cannot develop into adults. Such growth regulators have little effect on fish, peaks and dragonflies and butterflies, and are high in cost. Long-term use of existing insecticides or growth regulators can easily induce mosquito resistance. At present, there are also methods for improving the penetration of insecticides, which cause suffocation by penetrating into mosquitoes, such as CN111820208A which uses silicone surfactants in combination with other additives to suffocate mosquito larvae in water; CN108184835A uses silicone surfactants in combination with other surface active penetration agents to kill mites; but the organic silicon surfactant is toxic to fish, aquatic organisms, livestock and other animals, which is not conducive to the breeding industry.

[0003] Existing methods also use a combination of physical and chemical methods to kill mosquitoes. For example, CN113519527A uses an organic acid and surfactant solution, which is atomized into an aerosol. The surfactant makes the aerosol adhere to the wings of mosquitoes, and the organic acid corrodes the wings of mosquitoes to knock down the mosquitoes. However, this technical solution uses too many raw materials, which is high in cost. In addition, the organic acid can corrode equipment and houses, and also poses a hazard to livestock.

[0004] CN109964932A uses a combination of surfactants such as sodium cocoyl glycinate, penetration agents such as methyl oleate, wetting agents such as isomeric decanol polyoxyethylene, and antioxidants such as 2,6-di-tert-butyl-4-methylphenol, which are diluted into a solution that quickly penetrates into the spiracles of insects and blocks them, causing suffocation and death of the pests. It is mainly used for the control of lepidopteran pest larvae and mites. CN104186463A discloses a water-based health insecticidal aerosol and its preparation method, which uses pyrethroid insecticides, surfactants, corrosion inhibitors, penetration agents, wetting agents, etc. These insecticides have complex compositions, and the additional use of penetration agents and wetting agents increases the cost.

[0005] In summary, the existing insecticide mainly uses pyrethroid, organosilicon surfactant or other surfactant compound, in order to increase the insecticidal effect, the additional penetration agent and wetting agent and other substances are generally added, the cost is high, and the formula is complex. Therefore, it is necessary to provide an environmentally friendly insecticide with high efficiency, low toxicity, avoiding drug resistance and reducing cost. SUMMARY

[0006] In order to solve the above problems, the present application provides an insecticide containing gemini surfactant, which kills mosquitoes, flies and mosquitoes by physical principle, has less dosage, low cost and environmental friendliness.

[0007] In order to achieve the above purpose, the present application is realized by the following technical scheme:

[0008] An insecticide containing gemini surfactant, comprising, in terms of mass percentage:

[0009] (a) 0.5-1 ‰ of gemini surfactant, the structural formula of the gemini surfactant is:

[0010] wherein R1 is C6-C8 alkyl, R2 is C2-C4 alkyl; Z + is any one of Na + , K + , NH4 + ;

[0011] (b) 0.3-0.5 ‰ of synergist;

[0012] (c) 0-0.3 ‰ of auxiliary material;

[0013] (d) the balance is water.

[0014] Further, R1 is any one of n-hexyl, n-octyl, iso-octyl and sec-octyl.

[0015] Further, the synergist is ricinoleic acid.

[0016] Further, the auxiliary material is any one or several of citric acid, sodium citrate, plant essential oil.

[0017] Further, the plant essential oil includes one or several of eucalyptus leaf essential oil, mint essential oil, clove essential oil, rose essential oil, lemon essential oil, jasmine essential oil, orange peel essential oil, tea tree essential oil, cinnamon essential oil, citronella essential oil, wormwood essential oil, chamomile essential oil.

[0018] Further, the preparation method of the insecticide is that the gemini surfactant, the synergist, the auxiliary material and the water are fully mixed into a uniform liquid, and the insecticide is obtained.

[0019] The application also discloses application of the insecticide containing the gemini surfactant in preventing and treating mosquitoes, flies and gnats in poultry and livestock farms.

[0020] The insecticide containing the gemini surfactant and the application have the beneficial effects that:

[0021] (1) The insecticide mainly adopts sulfonate anionic gemini surfactant and castor oil acid as main acting components. The gemini surfactant forms asymmetric anionic gemini surfactant because of the existence of ester groups and amide groups. The amide group belongs to a polar group. The surfactant containing the amide group is more easily arranged closely through intermolecular hydrogen bonds or dipole moment interaction, thereby enhancing the surface activity and improving the wetting and penetration of the octyl sulfonate succinate aqueous solution. As a spraying insecticide, the insecticide is strongly attached to the wings and epidermis of mosquitoes, flies and gnats. The mosquitoes lose the flying ability and are knocked down due to the attachment and wetting of the insecticide. The castor oil acid affects the flight control and senses of the mosquitoes, flies and gnats, so that the mosquitoes, flies and gnats are difficult to fly and affect the foraging efficiency. The insecticide can further make the flies, which are larger than the mosquitoes, reject food and finally die, thereby improving the killing rate of the insecticide. The gemini surfactant can quickly penetrate into the body of the mosquitoes, and cooperates with the fatty acid castor oil acid to close the spiracles in the body of the mosquitoes, flies and gnats, so that the mosquitoes, flies and gnats are suffocated to death.

[0022] (2) The gemini surfactant contained in the insecticide has strong wetting and penetration, so that the wetting agent and the penetration agent do not need to be additionally added in the formula of the insecticide. The use amount of the gemini surfactant is small. The formula is simplified under the premise of the same insecticidal effect, and the cost of the insecticide is reduced.

[0023] (3) The gemini surfactant contains ester groups and amide groups. After the amide group is introduced, the toxicity is greatly reduced. The gemini surfactant does not contain chlorine ions, fluorine and other substances, and belongs to an anionic surfactant. The toxicity of the insecticide is further reduced, and the content is small. The influence on the human body and the livestock and poultry is small.

[0024] (4) The insecticide adopts the physical principle to kill the mosquitoes, flies and gnats. There is no drug resistance, and the insecticide can be repeatedly used for a long time. DETAILED DESCRIPTION

[0025] In order to enable personnel in the art to better understand the present application, the technical solutions of the present application will be described clearly and completely in combination with embodiments. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the present application.

[0026] An insecticide containing a gemini surfactant, comprising, in percentage by mass:

[0027] (a) 0.5-1‰ of a gemini surfactant, the gemini surfactant having a structural formula of:

[0028] wherein R1 is a C6-C8 alkyl group, R2 is a C2-C4 alkyl group; Z is any one of Na + , K + , NH4 + , and +

[0029] (b) 0.3-0.5‰ of a synergist castor oil acid;

[0030] (c) 0-0.3‰ of an auxiliary;

[0031] (d) the balance being water.

[0032] It is further explained that R1 is any one of a n-hexyl group, a n-octyl group, an iso-octyl group, and a sec-octyl group.

[0033] It is further explained that the method for preparing the gemini surfactant in the present application comprises the following steps:

[0034] ① reacting a C2-C4 alkyl alcohol amine with maleic anhydride in the presence of a catalyst sodium acetate and a solvent at a temperature of 60-100℃ for 2h to obtain an intermediate through amidation and esterification;

[0035] ② adding a C6-C8 fatty alcohol and a solid acid catalyst to the intermediate, and performing double esterification at a temperature of 120-180℃ to obtain a gemini maleic acid double ester;

[0036] ③ adding a sulfonating agent sodium bisulfate and a catalyst promoter to the gemini maleic acid double ester in the presence of water, and performing sulfonation at a temperature of 100-120℃ for 6h to obtain a sulfonate gemini surfactant.

[0037] In step ①, the C2-C4 alkyl alcohol amine is any one of ethanolamine, propanolamine, and butanolamine; and the molar ratio of the C2-C4 alkyl alcohol amine to maleic anhydride is 1:2.0-2.5. In this embodiment, the molar ratio of the C2-C4 alkyl alcohol amine to maleic anhydride is preferably 1:2.1.

[0038] In step ①, the amount of the catalyst sodium acetate added is 0.5-1% of the total mass of the C2-C4 alkyl alcohol amine and maleic anhydride. In this embodiment, the amount of the catalyst sodium acetate added is preferably 0.5% of the total mass of the C2-C4 alkyl alcohol amine and maleic anhydride.

[0039] ​The solvent in step 1 is cyclohexane, or n-heptane, or toluene, or xylene; preferably toluene in this embodiment.

[0040] The C6-C8 fatty alcohol in step 2 is any one of n-hexanol, n-octanol, iso-octanol and sec-octanol; preferably any one of iso-octanol and sec-octanol; the addition amount of the C6-C8 fatty alcohol is 1.1 moles per mole of maleic anhydride; the solid acid catalyst is HND-26, and the addition amount of the solid acid catalyst is 0.5-3% of the weight of maleic anhydride. The addition amount of the solid acid catalyst is preferably 2% of the weight of maleic anhydride in this embodiment.

[0041] The sulfonating agent in step 3 is any one of sodium bisulfite, potassium bisulfite and ammonium bisulfite, and the sulfonating agent is preferably sodium bisulfite in this embodiment. The addition amount of the sulfonating agent is 1-1.2 moles of sodium bisulfite per mole of maleic anhydride, and the most preferred addition amount is 1.05 moles of sodium bisulfite per mole of maleic anhydride.

[0042] The co-catalyst in step 3 is a mixture of ethanol and dimethyl sulfoxide in a molar ratio of 1:1; the addition amount of the co-catalyst is 0.3-0.6% of the weight of the gemini maleic acid bis-ester. The addition amount of the co-catalyst is preferably 0.5% of the weight of the gemini maleic acid bis-ester in this embodiment.

[0043] The addition amount of water in step 3 is 1.2 times the weight of maleic anhydride.

[0044] It is further explained that the auxiliary is any one or several of citric acid, sodium citrate and plant essential oil. The plant essential oil includes any one or several of eucalyptus essential oil, mint essential oil, clove essential oil, rose essential oil, lemon essential oil, jasmine essential oil, orange peel essential oil, tea tree essential oil, cinnamon essential oil, citronella essential oil, wormwood essential oil and chamomile essential oil.

[0045] It is further explained that the preparation method of the insecticide containing the gemini surfactant is to mix, dissolve and uniformly mix the gemini surfactant, the synergist, the auxiliary and water to obtain the insecticide.

[0046] Table 1 is the specific components of the insecticide containing the gemini surfactant according to the mass percentage of Examples 1-18 of the present application.

[0047] Table 1 is the specific components of the insecticide containing the gemini surfactant according to the mass percentage of Examples 1-18 of the present application.

[0048]

[0049]

[0050] Comparative Example 1

[0051] Insecticide, using an aqueous solution of amitraz at a concentration of 1 ‰.

[0052] Insecticide preparation method in this comparative example: amitraz technical was added to water, and stirred at room temperature for 1 hour to obtain an aqueous solution.

[0053] Comparative Example 2

[0054] Insecticide, using an aqueous solution of organosilicon at a concentration of 1 ‰.

[0055] Comparative Example 3

[0056] Insecticide, using an aqueous solution of tefluthrin at a concentration of 1 ‰.

[0057] Insecticide preparation method in this comparative example: tefluthrin technical was added to water, and stirred at room temperature for 1 hour to obtain an aqueous solution.

[0058] Comparative Example 4

[0059] Insecticide, using an aqueous solution of imiprothrin at a concentration of 1 ‰.

[0060] Insecticide preparation method in this comparative example: imiprothrin technical was added to water, and stirred at room temperature for 1 hour to obtain an aqueous solution.

[0061] Comparative Example 5

[0062] Insecticide, using an aqueous solution of chlorofenapyr at a concentration of 1 ‰.

[0063] Insecticide preparation method in this comparative example: chlorofenapyr technical was added to water, and propylene glycol was added as a solvent, the mass of propylene glycol being 15% of the mass of the insecticide, and stirred at room temperature for 1 hour to obtain an aqueous solution of chlorofenapyr.

[0064] Comparative Example 6

[0065] Insecticide, including 1 ‰ of Gemini surfactant, the remainder being water, wherein the Gemini surfactant is the same as in Example 1.

[0066] Comparative Example 7

[0067] Insecticide, including 1.3 ‰ of Gemini surfactant, the remainder being water, wherein the Gemini surfactant is the same as in Example 1.

[0068] Comparative Example 8

[0069] Insecticide, including 1.3 ‰ of ricinoleic acid, the remainder being water.

[0070] Insecticide preparation method in this comparative example: ricinoleic acid was added to water, and stirred at room temperature for 1 hour to obtain an aqueous solution.

[0071] The blank group is clean water.

[0072] Test detection

[0073] I. Mosquito killing effect

[0074] The GB_T 13917.1-2009 "Pesticide registration indoor efficacy test and evaluation of health insecticide Part 1 Spray" 4.2 spray experiment is used to verify the killing effect. 30 mosquitoes are placed in different cylinders, and the insecticides obtained from Examples 1-18 and Comparative Examples 1-8 are used for spray experiments. According to the effective dose of 1.43 ml / m 3 The liquid medicine is sprayed into the cylinder, and the round hole is immediately plugged with a rubber plug. After 1 min, the pull plate is pulled out and the time is counted, and the number of knocked down test insects at each time is recorded. After 20 min, the test insects in the cylinder are transferred to different clean containers, and the normal feeding is restored. The number of dead test insects in each clean container is observed for 72 h, and the time for 50% of the mosquitoes to be knocked down (KT 50 ) and the mortality rate are calculated. The test is repeated 3 times, and a blank control group is set. The results are shown in Table 2.

[0075] Table 2 Killing effect of different insecticides on mosquitoes

[0076]

[0077]

[0078] Note: "-" in Table 2 represents that the number of knockdowns is always less than 50%, and there is no data.

[0079] From Table 2, it can be seen that the KT 50 of the insecticide used in Examples 1-18 is within 3 min, most of which is within 2 min, and the killing effect of Examples 1, 2, 6-10 on mosquitoes is better, and the KT 50 is within 1 min, which has a high and fast killing effect.

[0080] From Examples 1 and Comparative Examples 6-9, it can be seen that only the addition of the gemini surfactant of the present application has a higher mosquito killing effect than the conventional mosquito killing component of the prior art, but after the addition of ricinoleic acid, the mosquito killing effect of the gemini surfactant can be further improved. The mosquito killing effect of the insecticide containing only ricinoleic acid (Comparative Example 8) is poor because ricinoleic acid is difficult to dissolve in water and cannot be dispersed, and cannot achieve the killing effect. This shows that the simple gemini surfactant has limited killing effect, and the simple ricinoleic acid has no killing effect. The insecticide of the present application has the effect of solubilizing and dispersing ricinoleic acid by the gemini surfactant, and the effect of synergizing the gemini surfactant by ricinoleic acid, and the combination of the two achieves a synergistic effect, and has a good mosquito killing effect.

[0081] II. Fly killing effect

[0082] The GB_T 13917.1-2009 "Pesticide registration for indoor efficacy test and evaluation of hygienic insecticide Part 1: Spray agent", 4.2 spray experiment was used to verify the killing effect. 30 flies were respectively placed in different cylinders, and the insecticides obtained from the examples 1-18 and the comparative examples 1-8 were used for the spray experiment. The liquid medicine was sprayed into the cylinder according to the effective dose of 1.43 ml / m 3 2, and immediately the round hole was plugged with a rubber plug. After 1 min, the pull plate was pulled out and timing was started, and the number of flies knocked down at each time was recorded. After 20 min, the flies in the cylinder were transferred to different clean vessels, and normal feeding was resumed. The number of dead flies in each clean vessel was observed for 72 h, and the time for 50% flies to be knocked down (KT 50 ) and the mortality rate were calculated. The experiment was repeated for 3 times, and a blank control group was set. The results are shown in Table 3:

[0083] Table 3 Killing effect of different insecticides on flies

[0084] Group KT 50 (MIN) 24h mortality 48h mortality 72h mortality Example 1 1.36 92 100 100 Example 2 1.50 91 100 100 Example 7 1.45 93 100 100 Example 8 1.54 90 100 100 Example 9 1.65 84 100 100 Example 11 2.77 83 100 100 Example 12 2.86 81 100 100 Example 17 2.33 86 100 100 Example 18 2.29 85 100 100 Comparative Example 1 - 14 21 21 Comparative Example 2 2.85 36 40 40 Comparative Example 3 - 9 13 17 Comparative Example 4 - 5 5 7 Comparative Example 5 3.47 76 92 100 Comparative Example 6 1.51 73 76 77 Comparative Example 7 1.43 75 77 77 Comparative Example 8 - 0 0 0 Blank Group - 0 0 0

[0085] Note: "-" in Table 3 represents that the number of flies knocked down is always less than 50%, and there is no data.

[0086] III. Killing effect of midges

[0087] The GB_T 13917.1-2009 "Pesticide registration for indoor efficacy test and evaluation of hygienic insecticide Part 1: Spray agent", 4.2 spray experiment was used to verify the killing effect. 30 flies were respectively placed in different cylinders, and the insecticides obtained from the examples 1-18 and the comparative examples 1-8 were used for the spray experiment. The liquid medicine was sprayed into the cylinder according to the effective dose of 1.43 ml / m 3 2, and immediately the round hole was plugged with a rubber plug. After 1 min, the pull plate was pulled out and timing was started, and the number of flies knocked down at each time was recorded. After 20 min, the flies in the cylinder were transferred to different clean vessels, and normal feeding was resumed. The number of dead flies in each clean vessel was observed for 72 h, and the time for 50% flies to be knocked down (KT 50 ) and the mortality rate were calculated. The experiment was repeated for 3 times, and a blank control group was set. The results are shown in Table 3:

[0088] Table 4 Killing effect of different insecticides on midges

[0089] Group KT 50 (MIN) 24h mortality 48h mortality 72h mortality Example 1 0.73 100 100 100 Example 2 0.68 100 100 100 Example 7 0.58 100 100 100 Example 8 1.54 100 100 100 Example 9 1.65 100 100 100 Example 11 2.77 100 100 100 Example 12 2.86 100 100 100 Example 17 2.33 100 100 100 Example 18 2.29 100 100 100 Comparative Example 1 9.64 30 34 42 Comparative Example 2 2.85 73 76 81 Comparative Example 3 8.33 16 16 16 Comparative Example 4 7.73 18 18 18 Comparative Example 5 4.06 95 100 100 Comparative Example 6 0.65 87 87 87 Comparative Example 7 0.59 87 90 91 Comparative Example 8 - 0 0 0 Blank Group - 0 0 0

[0090] Note: "-" in Table 4 represents that the number of flies knocked down is always less than 50%, and there is no data.

[0091] As can be seen from Table 3 and Table 4, Examples 1-18 have good killing effect on mosquitoes, flies and gnats, but Comparative Examples 1, 3 and 4 have poor effect, especially the knockdown effect on flies is lower than 50%, which does not meet the standard. Pyrethrum raw material is not soluble in water, and it is difficult to mix uniformly by stirring directly, resulting in low insecticidal effect, large amount of use, low utilization rate of insecticidal ingredients, and environmental pollution by large amount of use. In the actual use process, mosquito incense liquid needs to add low alcohol solvents such as ethanol and propylene glycol, and aerosol needs to add aromatic solvents, which increases the cost. After Comparative Example 5 is mixed with propylene glycol to form a homogeneous solution, the effect is obviously improved, but the knockdown rate is still lower than that of Examples 1-18.

[0092] As can be seen from Comparative Example 6, without adding the synergist ricinoleic acid, the gemini surfactant can knock down the experimental pests more quickly, but the subsequent killing effect is insufficient. Especially for larger insects such as flies, it is difficult to achieve complete killing under the standard spraying amount.

[0093] The gemini surfactant helps ricinoleic acid to disperse in water and assists ricinoleic acid to spread on the body surface after contacting with insects. Ricinoleic acid changes the flight and feeding of insects to achieve the killing effect.

[0094] Four, toxicology test

[0095] (1) According to GB / T 31270.12-2014 Chemical Pesticides Environmental Safety Evaluation Experimental Guidelines-Part 12: Fish Acute Toxicity Test, the 96h median lethal concentration of carp is used to detect the acute fish toxicity of the compound.

[0096] Experimental object: 100 adult fish of carp;

[0097] Experimental drug: gemini surfactant in Examples 1-18.

[0098] Test result: no death of carp was observed at the concentration of 100 mg a.i. / L of gemini surfactant in Examples 1-18, and it is determined that the gemini surfactant of the application is non-toxic.

[0099] (2) According to GB / T 31270.12-2014 Chemical Pesticides Environmental Safety Evaluation Experimental Guidelines-Part 20: Short-term Feeding Toxicity Test of Livestock, the 24d median lethal concentration of pigs is used to detect the acute toxicity of the compound.

[0100] Experimental object: 5 adult mature pigs;

[0101] Experimental drug: gemini surfactant in Examples 1-18.

[0102] Test results: No death of piglets was observed at 5000 mg / kg of the gemini surfactants in Examples 1-18, which determined that the gemini surfactants of the present application were non-toxic.

[0103] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive of one another. As will be apparent to those of ordinary skill in the art, embodiments described herein can be combined with one another.

[0104] Finally, it should be noted that: the embodiments disclosed in the present application are only the preferred embodiments of the present application, and are only used to explain the technical solutions of the present application, not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand; the technical solutions recorded in the foregoing embodiments can be modified, or some technical features can be replaced equivalently; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An insecticide comprising a gemini surfactant, characterized in that: According to the mass percentage, it comprises: (a) 0.5-1‰ of Gemini surfactant, the structural formula of which is: wherein R1 is a C6-C8 alkyl group, said R2 is a C2-C4 alkyl group; said Z + is any one of Na + , K + , NH4 + . (b) 0.3-0.5‰ of synergist; (c) 0-0.3‰ of adjuvant; (d) the balance is water.

2. The biogenic surface active agent containing insecticide according to claim 1, wherein: The R1 is any one of n-hexyl, n-octyl, iso-octyl and sec-octyl.

3. The biogenic surface active agent containing insecticide as claimed in claim 1 wherein: The synergist is ricinoleic acid.

4. The biogenic surface active agent containing insecticide as claimed in claim 1 wherein: The adjuvant is any one or several of citric acid, sodium citrate and plant essential oil.

5. The biogenic surface active agent containing insecticide as claimed in claim 4 wherein: The plant essential oil comprises one or several of eucalyptus leaf essential oil, mint essential oil, clove essential oil, rose flower essential oil, lemon essential oil, jasmine flower essential oil, orange peel essential oil, tea tree essential oil, cinnamon essential oil, citronella essential oil, wormwood leaf essential oil and chamomile essential oil.

6. The process for the preparation of a biogenic surface active agent containing insecticide as claimed in claim 1 wherein: The Gemini surfactant, the synergist, the adjuvant and the water are mixed thoroughly into a uniform liquid, and the insecticide is obtained.

7. The use of the insecticide containing Gemini surfactant according to any one of claims 1-6 in the prevention and treatment of mosquitoes, flies and gnats in poultry and livestock farms.

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