Temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device

Abstract

The application discloses a temperature compensation type electrode pluggable microbial electrolysis cell water quality sensing device, which comprises a microbial electrolysis cell, a temperature sensor, an electrochemical workstation and an upper computer. The microbial electrolysis cell adopts a three-electrode system, the electrochemical workstation is used for collecting original current signals related to water quality output by the microbial electrolysis cell and transmitting original electric signal data to the upper computer. The temperature sensor is used for detecting the temperature of a solution in a working electrode area and transmitting a temperature analog signal to a temperature acquisition card for conversion, and transmitting converted digital signals to the upper computer. The upper computer establishes a temperature compensation model based on the characteristics of electrochemical microorganisms, calculates current signals after temperature compensation according to original current signals and measured temperature data, and judges water quality conditions according to the current signals after compensation.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of water quality detection, and particularly relates to a temperature compensation type electrode pluggable microbial electrolysis cell water quality sensing device. BACKGROUND

[0002] Water is the source of life, and water quality detection is of vital importance to the protection of human life and health. Traditional water quality detection methods require professional personnel to collect samples and conduct laboratory analysis, which is time-consuming and labor-intensive and the results are not real-time, and cannot meet the real-time and accuracy requirements of water quality monitoring.

[0003] In recent years, water quality toxicity detection based on electrochemical microorganisms has gradually become one of the research hotspots in the field of water quality detection. Microbial electrochemical sensors are a kind of microbial electrochemical technology (MET) that can sense through anodic or cathodic electroactive microorganisms and / or biological membranes; electrochemical microorganisms are a kind of microorganisms that can generate electric current by utilizing organic matter, and their application in water quality detection has broad prospects.

[0004] In the electrolytic cell water quality sensor based on electrochemical microorganisms, it includes a working electrode, a counter electrode, and a reference electrode, electrochemical microorganisms are attached to the working electrode, and by applying a constant potential to the working electrode, toxic pollutants in the water body will inhibit the respiratory and metabolic activity of electrochemical microorganisms, thereby causing the electric signal to decrease, and the resistance of EAB to toxic pollutants directly determines the sensitivity of the water quality detection. By analyzing the change in the metabolic current of the microorganisms, the water quality condition can be judged; however, electrochemical microorganisms are sensitive to temperature, and their output current will fluctuate under different temperature conditions, affecting the water quality detection accuracy of the sensor. The respiratory and metabolic activity of EAB is affected by toxic substances in water, and the electric signal changes. In the sensitive range of electrochemical microorganisms, temperature fluctuations will also affect the respiratory and metabolic activity of microorganisms; therefore, it is necessary to master the sensitivity law of microorganisms to temperature in order to improve the detection accuracy and reliability of the electrolytic cell water quality sensor based on electrochemical microorganisms;

[0005] The microbial electrode of the electrochemical microbial water quality sensor is the core component of the sensor, and its performance directly determines the accuracy and service life of the sensor. The existing microbial electrode of the electrochemical microbial sensor is mostly fixedly installed, that is, the microbial electrode is directly fixed in the electrode chamber of the electrolytic cell. When the electrode is aged, the microorganisms die, and the colony results change cannot meet the working requirements, the disassembly and replacement process is relatively cumbersome; at the same time, every time the electrode is replaced, the operation process introduces uncertain factors, which adversely affects the consistency of the sensitive element.

[0006] Therefore, temperature compensation and optimization design of the electrolytic cell structure can reduce the influence of temperature change on the output signal of the water quality sensor, obtain higher quality and more reliable sensing signal, and help improve the detection accuracy of the electrochemical microbial water quality sensor. SUMMARY

[0007] To solve the above technical problems, the purpose of the present application is to provide a temperature compensation type electrode pluggable microbial electrolysis cell water quality sensing device.

[0008] The purpose of the present application is achieved by the following technical solutions:

[0009] A temperature compensation type electrode pluggable microbial electrolysis cell water quality sensing device, comprising: a microbial electrolysis cell, a temperature sensor, an electrochemical workstation and an upper computer;

[0010] The microbial electrolysis cell adopts a three-electrode system, and the working electrode in the microbial electrolysis cell is attached with electrochemical microorganisms. The metabolic process produces electrons, and the electrochemical workstation collects the water quality related electrical signals output by the microbial electrolysis cell, and transmits the original current signal data to the upper computer;

[0011] The temperature sensor is used for detecting the temperature of the solution in the working electrode area, and transmitting the temperature analog signal to the temperature acquisition card for conversion, and transmitting the converted digital signal to the upper computer;

[0012] The upper computer establishes a temperature compensation model based on the characteristics of electrochemical microorganisms, calculates the temperature compensated current signal according to the original current signal and the measured temperature data, and judges the water quality condition according to the compensated current signal.

[0013] Compared with the prior art, one or more embodiments of the present application can have the following advantages:

[0014] The working electrode is embedded in the pluggable card slot, the pluggable card slot can be directly inserted or pulled out from the microbial electrolysis cell, the electrode can be conveniently replaced, and the long-term stability is beneficial; meanwhile, multiple electrode card slots can be used in parallel to balance the differences between the electrodes and improve the detection accuracy;

[0015] The compensated current signal is processed and analyzed by a water quality judgment analysis algorithm, and the water quality condition is output. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is a temperature compensation type electrode pluggable microbial electrolysis cell water quality sensing device diagram;

[0017] Figure 2 is a temperature compensation and water quality detection flowchart;

[0018] Figure 3 is a temperature compensation fitting curve diagram;

[0019] Figure 4 is a temperature compensation fitting curve diagram;

[0020] Figure 5 is a temperature compensation fitting curve diagram;

[0021] Figure 6 is a temperature sensor measurement electrode area temperature schematic diagram. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application will be further described in detail below with examples and drawings.

[0023] As shown in Figure 1 , it is a temperature compensation type electrode pluggable microbial electrolysis cell water quality sensing device, which comprises a microbial electrolysis cell 100, a temperature sensor 200, an electrochemical workstation 300 and an upper computer 500.

[0024] The microbial electrolysis cell adopts a three-electrode system, and the electrochemical workstation collects the original current signal related to water quality output by the microbial electrolysis cell, and transmits the original current signal data to the upper computer.

[0025] The temperature sensor is used to detect the temperature of the solution in the microbial electrode area, and transmits the temperature analog signal to the temperature acquisition card for conversion, and transmits the converted digital signal to the upper computer.

[0026] The upper computer establishes a temperature compensation model based on the electrochemical microbial characteristics, calculates the temperature compensated current signal based on the original current signal and the temperature measurement signal, obtains the compensated current signal, and calculates the water quality parameters according to the compensated current signal.

[0027] The above-mentioned three-electrode system includes a working electrode E101, a counter electrode E102 and a parameter electrode E103, wherein the working electrode E101 serves as a sensitive element for attaching electrochemical microorganisms and their growth, and at the same time, the electrons generated by the electrochemical microbial metabolism are transmitted to the external circuit through the working electrode, generating a current signal related to water quality. The three electrodes are measured by the electrochemical workstation.

[0028] The above-mentioned temperature sensor is arranged in the microbial electrolysis cell, which is used to detect the temperature of the solution in the working electrode area in real time, and output the temperature analog signal to the temperature acquisition card. The temperature acquisition card performs AD conversion on the temperature analog signal, and transmits the digital signal to the upper computer.

[0029] The electrochemical workstation is used for detecting the original current signal of the microbial electrolysis cell output related to water quality, and transmitting the original current data to the upper computer, and the upper computer simultaneously receives the temperature measurement signal from the temperature acquisition card and the original current signal from the electrochemical workstation.

[0030] The temperature measurement signal and the original current signal are input into the above-mentioned upper computer; the compensated current signal is output according to the pre-established temperature compensation model calculation; and the water quality condition is judged by analyzing the compensated current signal;

[0031] The temperature acquisition card and the electrochemical workstation respectively collect the temperature signal and the electrolysis cell current signal, and then input them into the upper computer for processing and calculation, so that the functions of temperature acquisition and electrochemical measurement can be well realized. The modular design also increases the reliability and stability of the system (such as Figure 2 ).

[0032] Temperature compensation: screening of electrochemically active microorganisms to form single bacterial group or complex community that meet the requirements of water quality detection;

[0033] The electrochemically active microorganisms are divided into multiple groups in parallel, and are cultured in different temperature environments, such as 10℃, 20℃, 30℃, 35℃, etc. The current signal data under different temperature conditions are collected; the current response curves under different temperature conditions are drawn as shown in Figure 2 ; the sensitive temperature of the microorganisms is set as the reference temperature Tb, the current density compensation parameter μ T under different temperatures is calculated, the relationship model between the actual temperature and the compensated current of the reference temperature is established, and the temperature current compensation curve is fitted (as shown in Figure 3 and Figure 4 ); the actual temperature T is input, and the compensated current is I=f(T);

[0034] Temperature compensation for electrolysis cell current change:

[0035] ΔI=dI-(μ T2 -μ Tb )*S

[0036] dI is the measured current change, μ T is the fitted current density, μ Tb is the current density at the reference temperature, and S is the electrode area.

[0037] As shown in Figure 5 and Figure 6As shown, the temperature compensation and electrode plug-in microbial electrolysis cell structure is composed of an inlet pipe 1, a clamping groove frame 2, an outlet pipe 3, an electrolysis cell body 4, a bent pipe interface 5, a working electrode E101, a counter electrode E102, a reference electrode E103, an electrode plug-in clamping groove 9, a temperature sensor 10, an upper end cover 11, a lower end cover 12, and a plug-in clamping groove electrode module 13; the inlet pipe is used for guiding water samples; the outlet pipe is used for discharging water samples; the upper end cover is used for closing the upper end opening of the microbial electrolysis cell; and the lower end cover is used for closing the lower end opening of the microbial electrolysis cell.

[0038] The plug-in clamping groove and the working electrode are assembled into a plug-in clamping groove electrode module, which is placed in the clamping groove frame to attach electrochemical microorganisms on the surface of the working electrode and culture the working electrode. After the plug-in clamping groove electrode module has the water quality detection function, the plug-in clamping groove electrode module is placed in the electrolysis cell body slot. When the output electrical signal of the microbial electrolysis cell fluctuates and the performance of the working electrode decreases, the plug-in clamping groove electrode module is taken out of the electrolysis cell body slot. The plug-in clamping groove electrode module with good function is taken out of the clamping groove frame and placed in the electrolysis cell body slot for water quality detection.

[0039] The plug-in clamping groove electrode module is placed in the clamping groove frame for culture, so that the obtained electrode, clamping groove, microbial attachment, and culture environment are consistent, the consistency of the electrolysis cell sensitive element, i.e., the working electrode, is improved, and the water quality detection accuracy and reliability are improved.

[0040] The end cover is connected with the cell body through threads, the temperature sensor is connected with the end cover through threads, and the end of the temperature sensor is about 3 cm away from the surface of the working electrode; and the plug-in clamping groove electrode module is sealed with the slot to prevent water leakage.

[0041] The above embodiment makes the microbial electrode into a plug-in clamping groove electrode, which can be conveniently inserted and pulled out of the electrolysis cell body structure. The plug-in structure can effectively solve the problem of electrode replacement. The operator can prepare multiple plug-in electrode clamping grooves in advance and culture microorganisms in the plug-in electrode clamping grooves to make the plug-in electrode clamping grooves have the water quality detection function. After the plug-in clamping groove electrode module is made, the plug-in clamping groove electrode module can be directly inserted into the microbial electrolysis cell for use. When the electrode is aged, the plug-in clamping groove electrode module can be directly pulled out for replacement without disassembling the electrolysis cell body. The plug-in electrode clamping groove structure can also realize parallel use of multiple electrodes to balance the differences between the electrodes and improve the consistency of the sensor.

[0042] The plug-in electrode clamping groove not only can realize quick disassembly and replacement of the electrode, but also can improve the sensor accuracy through parallel use of multiple electrodes, solve the inconvenience of electrode replacement and calibration, and has high practical value. The technical scheme belongs to improvement and perfection of the existing product and meets the requirements of patent protection.

[0043] Therefore, a method for real-time detection of solution temperature in the anode region of a microbial electrolysis cell by using a temperature sensor and compensation of output current signals of electrochemical microorganisms according to temperature characteristics of the microorganisms is proposed to improve water quality detection accuracy of the sensor. This method can effectively reduce the influence of temperature on water quality detection and improve the water quality detection accuracy of the sensor by accurately compensating the output current signals of electrochemical microorganisms through real-time monitoring of water temperature changes.

[0044] Although the embodiments of the present application are disclosed as above, the content is only for the purpose of understanding the embodiments adopted by the present application, and is not intended to limit the present application. Any person skilled in the art of the present application can make any modification and change in the form and details without departing from the spirit and scope of the present application. The patent protection scope of the present application shall be subject to the scope defined by the appended claims.

Claims

1. A temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device, characterized in that, Microbial electrolysis cell, temperature sensor, electrochemical workstation and host computer are included. The microbial electrolysis cell adopts a three-electrode system, and electrochemical microorganisms are attached to the working electrode in the microbial electrolysis cell. The metabolic process generates electrons, which are collected by the electrochemical workstation to generate an electric signal related to water quality. The original current signal data is transmitted to the host computer. The temperature sensor is used to detect the temperature of the solution in the working electrode area and transmit the temperature analog signal to the temperature acquisition card for conversion. The converted digital signal is transmitted to the host computer. The host computer establishes a temperature compensation model based on the characteristics of electrochemical microorganisms, calculates the current signal after temperature compensation according to the original current signal and measured temperature data, and judges the water quality according to the compensated current signal. I=f(T) The temperature compensation model sets the microbial sensitive temperature as the reference temperature Tb, calculates the current density compensation parameter μ at different temperatures T , and establishes a relationship model of the actual temperature and the reference temperature compensation current to fit a temperature current compensation curve; the input actual temperature T is compensated current The electrolysis cell current changes with temperature compensation: ; dI ； The microbial electrolysis cell water quality sensing device further includes a plug-in card slot and an electrolysis cell body. for the measured current change, μ T for the fitted current density, μ Tb for the current density at the reference temperature, S is the electrode area; The working electrode is embedded in the plug-in card slot to form a plug-in card slot electrode module. The electrolysis cell body is provided with an electrolysis cell slot on the side. The electrolysis cell slot is used to install the plug-in card slot electrode module. First, place the plug-in card slot electrode module in the card slot frame, attach electrochemical microorganisms to the surface of the working electrode, and culture to have water quality detection function. Then, place the cultured card slot electrode module into the slot of the electrolysis cell body for water quality detection. The microbial electrolysis cell includes a working electrode, a counter electrode and a reference electrode.

2. The temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device of claim 1, wherein, The working electrode is used to attach electrochemical microorganisms and grow in the working electrode area. The electrons generated by the metabolism of electrochemical microorganisms are transmitted through the working electrode and generate a current signal related to water quality. The reference electrode is used to provide a stable reference potential. The temperature acquisition card converts the temperature analog signal and transmits the converted digital signal to the host computer.

3. The temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device of claim 1, wherein, The temperature sensor is a thermocouple temperature sensor and is arranged in the microbial electrolysis cell.

4. The temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device of claim 1, wherein, The temperature compensation model based on the characteristics of electrochemical microorganisms includes:

5. The temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device of claim 1, wherein, Screening electrochemically active microorganisms that meet the requirements of water quality detection to form a single bacterial group or a complex community and attaching them to the working electrode for culture; Parallelly divide the electrochemical microorganisms into multiple groups, culture them in different temperature environments, collect current signal data under different temperature conditions, Draw the current response curve under different temperature conditions. The electrochemical workstation is used to collect the output signal of the electrolysis cell in real time, analyze the output signal to determine the performance of the working electrode, and determine whether the working electrode needs to be replaced according to its performance.

6. The temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device of claim 1, wherein, The microbial electrolysis cell water quality sensing device further includes an inlet pipe, an outlet pipe, an upper end cover and a lower end cover. The inlet pipe is used for water sample introduction. The outlet pipe is used for water sample discharge. The upper end cover is used to close the upper end opening of the microbial electrolysis cell. The lower end cover is used to close the lower end opening of the microbial electrolysis cell.

7. The temperature-compensated electrode pluggable microbial electrolysis cell water quality sensing device of claim 1, wherein, ​

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