A kind of cultivation medium of red canglong vine and selection method thereof

Abstract

The present invention provides a cultivation substrate for C. truncatum and a method for selecting the same. The method comprises mixing forest red soil, peat soil, and perlite in varying proportions, planting C. truncatum seedlings in the cultivation substrate, and measuring their growth in the second, fourth, and sixth months after planting. Ultimately, the cultivation substrate most suitable for C. truncatum growth is selected. Planting C. truncatum in this substrate significantly increases the yield of the plant.

Description

Technical field

[0001] The invention belongs to the field of plant spray culture substrates and relates to a safflower cultivation substrate and a selection method thereof. [Background Technology]

[0002] Erythropalum scandens Blume is a large, perennial, evergreen woody vine of the Aspergillus family and the genus Erythropalum. It is a natural, wild plant used for both medicinal and edible purposes. Its young leaves, nutritious and delicious, possess a unique fragrance. Its stems and roots are medicinal. The stems act as a diuretic, treating jaundice and rheumatic bone pain, while the roots relieve edema and treat injuries. Due to the increasing market demand for Erythropalum scandens Blume in recent years and the severe depletion of its wild germplasm resources, artificial cultivation is currently a key component of its production and promotion.

[0003] Currently, artificial propagation of Ficus microcarpa is primarily based on cuttings, but most cuttings suffer from underdeveloped root systems. Research has shown that Ficus microcarpa requires a loose, well-drained, and nutrient-rich cultivation medium. Therefore, the choice and proportion of the medium are key factors in determining the growth of Ficus microcarpa. Therefore, identifying the optimal cultivation medium ratio for Ficus microcarpa growth and effectively increasing its yield is a pressing technical challenge. [Summary of the invention]

[0004] In order to solve the above problems, the present invention provides a cultivation matrix for red sorghum vine, which is made of forest red soil, peat soil and perlite in different proportions. The growth of the planted red sorghum vine is measured, and finally the cultivation matrix most suitable for the growth of red sorghum vine is selected. The yield of red sorghum vine cultivated using this matrix is ​​greatly improved.

[0005] The present invention is achieved through the following technical solutions, providing a cultivation medium for Rhizoma Coptidis and a method for selecting the same, comprising the following steps:

[0006] S1 configures the cultivation medium;

[0007] S2: Planting the Caulis Aristolochiae Seedlings in the cultivation medium;

[0008] S3 measured the growth of Caulis et Rhizoma;

[0009] S4 Select the optimal cultivation medium for Rhizoma Coptidis.

[0010] Particularly, the cultivation medium is made of forest red soil, peat soil and perlite in a ratio of (1-2): (0-2): (0-2).

[0011] Particularly, the cultivation matrix is ​​made of forest red soil, peat soil and perlite in a ratio of 1:0:0 or 1:1:0 or 1:0:1 or 2:1:0 or 2:0:1 or 1:2:0 or 1:0:2 or 1:1:1.

[0012] Particularly, the cultivation medium is made of mountain red soil and peat soil in a ratio of 2:1.

[0013] Particularly, the particle size of the perlite is 3-6 mm.

[0014] In particular, the S2 is implemented according to the following scheme: each cultivation substrate is treated with 10 biological replicates, with one red sorghum seedling per replicate; after the red sorghum seedlings are transplanted, they are placed under a layer of shade net with a shading rate of 66.7%, and watered once every two days in summer and twice a week in autumn during the growth period of the seedlings. In the present invention, the non-woven bag has the characteristics of good air permeability and fast rooting, which can better promote the development of the root system of the red sorghum seedlings, and thus promote the growth of the plants. At the same time, it also has the advantages of low price and light weight. Therefore, the present invention places the cultivation substrates in 25cm×25cm non-woven bags respectively.

[0015] In particular, the S3 is implemented in accordance with the following scheme:

[0016] S31 measured the above-ground growth indicators of the Rhizoma Coptidis seedlings at 2, 4, and 6 months after transplanting;

[0017] Six months after transplanting S32, the root structure morphology and biomass of S.

[0018] In particular, in said S31, the ground diameter of the red cang tung and the new branches are measured with an electronic vernier caliper, with the results accurate to 0.01 mm, the total length of the new branches is measured with a steel ruler, with the results accurate to 0.01 cm, and the number of new branches, the total number of new leaves, and the total number of internodes are recorded by visual observation.

[0019] In particular, the S32 is specifically implemented as follows:

[0020] Root morphological structure measurement of S321: Three S. erythroxylum seedlings were randomly selected from each treatment and scanned with a root scanner. The results were analyzed using WHIZO software to measure the total root length, total root surface area, total projected area, root diameter, total volume, and number of root tips.

[0021] S322 Biomass determination: Three seedlings of Rhizoma Coptidis were randomly dug out for each treatment, washed and dried with filter paper, and the roots, stems and leaves of Rhizoma Coptidis were weighed for fresh weight respectively. After weighing, the fresh roots, stems and leaves were placed in an oven at 105℃ for 30 minutes, then dried at 75℃ and weighed for dry weight. Both fresh weight and dry weight should be accurate to 0.01g.

[0022] In particular, the S4 is implemented as follows:

[0023] S41: statistics are collected on the aboveground growth indicators of the Caulis et Rhizoma Coptidis seedlings, including the ground diameter increment, the mean new branch thickness, the total length of new branches, the total number of new branches, the total number of new branch leaves, the total number of internodes, and the average internode length. The ground diameter increment is the difference between the final ground diameter value and the initial ground diameter value. The mean new branch thickness is the average of three new branch thickness measurements. The total length of new branches is the sum of three new branch length measurements. The total number of new branches is the sum of three new branch numbers. The total number of new branch leaves is the sum of three new leaf numbers. The total number of internodes is the sum of three internode measurements. The average internode length is the quotient of the total length of new branches divided by the total number of internodes.

[0024] S42 counted the biomass of R. chinensis seedlings, including the fresh weight and total fresh weight of roots, stems and leaves, and the dry weight of roots, stems, leaves and leaves;

[0025] S43 counted the underground growth indicators of R. chinensis seedlings, including total root length, total root surface area, total projected area, root diameter, total volume, and number of root tips;

[0026] S44 sorted out the obtained data and performed variance analysis on the data of various indicators, and then used Duncan's new multiple range method to perform multiple comparisons, so as to select the substrate ratio most suitable for the cultivation of Red Cang Teng.

[0027] The present invention provides a cultivation medium for Rhizoma Coptidis and a method for selecting the same, which has the following beneficial effects:

[0028] It can accurately find the best combination of growth substrates suitable for red vine, further optimize the cultivation technology, provide a certain theoretical basis and technical support for red vine cultivation in future production, and select the best growing plants through statistical analysis of above-ground growth indicators, root system composition and biomass, which can provide higher red vine yields. [Specific implementation method]

[0029] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific embodiments and experiments.

[0030] The selection method of the described Caulis Aristolochiae Caulis cultivation substrate is as follows:

[0031] Step 1 Experimental design and experimental methods:

[0032] A three-factor completely randomized block experiment was conducted using forest red soil, peat soil, and perlite mixed in varying percentages to create a seedling mix (see Table 1). Eight treatments were selected: CK, T1, T2, T3, T4, T5, TT6, and T7. Each treatment was randomly assigned, with 10 biological replicates per treatment, for a total of 80 plants. The forest red soil was collected from the Nanning Arboretum in Guangxi Zhuang Autonomous Region; the peat soil was purchased from Changchun Yinong Saishi Peat Development Co., Ltd.; and the perlite, with a particle size of 3–6 mm, was purchased from Nanning Guiyuxin Agricultural Science and Technology Co., Ltd. The test seedlings were two-year-old cuttings of Caulis Aristolochiae var. stylophorae, provided by the Rare Tree Species and Flower Seedlings Breeding Center of the Nanning Arboretum. Cuttings were made from one-year-old shoots and pruned after survival. The experimental medium was purchased from Guiyuxin Agricultural Science and Technology Co., Ltd., Guangxi Agricultural Science and Technology Market. Plants were watered every two days in summer and twice weekly in autumn.

[0033] Table 1 Ratios of different substrates for Caulis et Rhizoma Coptidis seedlings

[0034]

[0035] Step 2: Select the experimental address and experimental container:

[0036] This experiment was conducted at the Guangxi University Forestry College Nursery Teaching and Practice Base (108°22′E, 22°48′N), located south of the Tropic of Cancer. The climate is characterized by a humid subtropical monsoon climate, with an average annual temperature of 21.6°C and rainfall of 1304 mm. After transplanting, the seedlings of the red vine were placed under a shade net with a 66.7% shading rate. The 25 cm × 25 cm non-woven bags selected for this experiment are characterized by good air permeability and rapid rooting, which can better promote the root development of the red vine seedlings and thus promote plant growth. They are also inexpensive and lightweight.

[0037] Step 3: Survey of aboveground growth indicators:

[0038] The above-ground growth indices of all the test seedlings (Caulis et Rhizoma Caulis seedlings) were measured at 2, 4, and 6 months after transplanting. The ground diameter and the thickness of new branches were measured using an electronic vernier caliper, with the results accurate to 0.01 mm; the total length of new branches was measured using a steel ruler, with the results accurate to 0.01 cm; the number of new branches, the total number of leaves on new branches, and the total number of internodes were recorded by visual observation.

[0039] Step 4: Determination of root morphology indicators:

[0040] At the end of the experiment at the sixth month after transplanting, three cuttings of Rhizoma Coptidis were randomly selected from each treatment and scanned with a root scanner. The results were analyzed using WHIZO software to measure the total root length, total root surface area, total projected area, root diameter, total volume, and number of root tips.

[0041] Step 5: Biomass index determination:

[0042] Six months after transplanting, three cuttings of P. serrata were randomly excavated from each treatment, washed with clean water, and dried with filter paper. The roots, stems, and leaves of the seedlings were separated and weighed for fresh weight. After the measurement, the fresh roots, stems, and leaves were placed in an oven at 105°C for 30 minutes, then dried at 75°C to constant weight. Dry weight was then measured (both fresh and dry weights were accurate to 0.01 g).

[0043] Step 6: Statistics and Analysis:

[0044] The aboveground growth indices of the test seedlings, including the ground diameter increment (the difference between the final ground diameter and the initial ground diameter), the mean new branch diameter (the average of three measurements of new branch diameter), the total length of new branches (the sum of the length of new branches measured three times), the total number of new branches (the sum of the number of new branches measured three times), the total number of new branch leaves (the sum of the number of new leaves measured three times), the total number of internodes (the sum of three measurements of internodes), and the average length of internodes (the quotient of the sum of the length of new branches divided by the total number of internodes) were counted; biomass, including the fresh weight of roots, stems, and leaves, the total fresh weight (the sum of the fresh weight of roots, stems, and leaves), the root, stem, leaf, and total dry weight (the sum of the dry weight of roots, stems, and leaves) were counted; underground growth indices, including the total root length, total root surface area, total projected area, root diameter, total volume, and number of root tips were counted as shown in Tables 2-9 below. Then, Excel 2016 software was used to organize the data, and the variance analysis of each indicator data was performed using SPSS (26.0). Then, Duncan's new multiple range method was used for multiple comparisons to find the most suitable substrate ratio for the cultivation of Red Cang Teng.

[0045] Table 2 CK statistics of treatment groups

[0046]

[0047] Table 3 T1 statistics of treatment groups

[0048]

[0049] Table 4 T2 statistics of treatment groups

[0050]

[0051] Table 5 T3 statistics of treatment groups

[0052]

[0053] Table 6 T4 statistics of treatment groups

[0054]

[0055] Table 7 T5 statistics of treatment groups

[0056]

[0057] Table 8 T6 statistics of treatment groups

[0058]

[0059] Table 9 T7 statistics of treatment groups

[0060]

[0061] By comparing the above table, we can see that:

[0062] Cultivating a mixture of mountain red soil and peat moss can promote the growth and biomass of C. arborescens. The mean values ​​of the membership functions are ranked as T3>T5>T1>CK>T2>T7>T6>T4. Experiments have shown that C. arborescens seedlings grow best when a 2:1 ratio of mountain red soil to peat moss is used as the cultivation medium. C. arborescens seedlings grow second best when a 1:2 ratio of mountain red soil to peat moss is used as the cultivation medium. If the cultivation medium does not contain peat moss, the growth of the seedlings is poor when the ratio of mountain red soil to perlite is 2:1. This indicates that peat moss is necessary for the cultivation medium.

[0063] In summary, the present invention also provides a cultivation medium suitable for the growth of C. truncatum, wherein forest red soil is acidic soil with heavy clay and low humus content. It is distributed in low hills below 700 meters above sea level north of the Tropic of Cancer and in mountains above 700 meters above sea level south of the Tropic of Cancer. It is the soil type with the largest distribution area in Guangxi and the main soil type in the natural distribution area of ​​C. truncatum. Peat soil is a combination of organic matter and humus accumulated over a long period of time. It is generally light in weight, has strong water absorption, and has high organic matter and humus content. It is an excellent soil improvement material. Perlite is low in cost and has good air permeability and inorganic properties. When forest red soil and peat soil are mixed in a ratio of 2:1 as the cultivation medium, the growth condition of C. truncatum seedlings is optimal. Therefore, it is the optimal ratio, and the yield of C. truncatum planted according to the above ratio is greatly improved.

Claims

1. A method for selecting a cultivation medium for Caulis Aristolochiae rhizome, characterized in that: The following steps are involved: S1: Prepare the cultivation medium; S2: Planting the Caulis Australis seedlings in a cultivation substrate. S2 is specifically implemented as follows: the cultivation substrates are placed in 25 cm × 25 cm non-woven bags, and each cultivation substrate is treated with 10 biological replicates, with one Caulis Australis seedling per replicate; after transplanting the Caulis Australis seedlings, they are placed under a layer of shade netting with a shading rate of 66.7%. During the growth period of the seedlings, water them once every two days in summer and twice a week in autumn; S3: Determine the growth of Rhizoma Coptidis; S4: selecting an optimal cultivation medium for the Rhizoma Coptidis, wherein the cultivation medium is made of forest red soil and peat soil in a ratio of 2:

1.

2. The method for selecting a cultivation medium for Caulis Aristolochiae Rhizoma according to claim 1, wherein: The S3 is specifically implemented according to the following scheme: S31 The aboveground growth indexes of the C. chinensis seedlings were measured at 2, 4, and 6 months after transplanting. S32 Six months after transplanting, the root structure morphology and biomass of S. erythrorhizon were measured.

3. The method for selecting a cultivation medium for Caulis Aristolochiae Rhizoma according to claim 2, wherein: In the S31, the ground diameter of the red cang tung tree and the thickness of the new branches are measured with an electronic vernier caliper, and the results are accurate to 0.01 mm. The total length of the new branches is measured with a steel ruler, and the results are accurate to 0.01 cm. The number of new branches, the total number of new leaves, and the total number of internodes are recorded by visual observation.

4. The method for selecting a cultivation medium for Caulis Aristolochiae Rhizoma according to claim 2, wherein: The S32 is specifically implemented as follows: S321 Root morphological structure measurement: Three Rhizoma Coptidis seedlings were randomly selected from each treatment and scanned with a root scanner. The results were analyzed using WHIZO software to measure the total root length, total root surface area, total projected area, root diameter, total volume, and number of root tips. S322 Biomass determination: Three seedlings of Rhizoma Cibotii were randomly dug out for each treatment, washed and dried with filter paper, and the roots, stems and leaves of Rhizoma Cibotii were weighed for fresh weight. After weighing, the fresh roots, stems and leaves were placed in an oven at 105℃ for 30 minutes, then dried at 75℃ and weighed for dry weight. Both fresh weight and dry weight should be accurate to 0.01g.

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