A biochemical sample sectioning apparatus and method

Abstract

The present application relates to the field of biochemistry, more particularly to a biochemical sample slicing device and method. The slicing device comprises a round seat, both sides of the round seat are fixed with middle frames, each middle frame is hinged with a rotating rod, and the lower part of each rotating rod is fixed with a fork. The slicing method comprises the following steps: S1: clamping the biological sample between the two forks; S2: immersing the biological sample in wax through the two forks; S3: driving the two clamps to clamp the biological sample and releasing the two forks; S4: alternately clamping the biological sample with the two forks and the two clamps, and wrapping the biological sample with wax layer by layer; S5: placing the biological sample wrapped with wax on the disc, moving the cutter left and right to cut the biological sample wrapped with wax, and slicing the biological sample layer by layer. The biological sample can be wrapped with wax layer by layer, and then sliced layer by layer.

Description

Technical Field

[0001] This invention relates to the field of biochemistry, and more specifically to a biochemical sample slicing device and slicing method. Background Technology

[0002] Biochemical sample sectioning is a common experimental technique typically used to study the interactions and structures between biomolecules. This process involves cutting biological tissues or cells into very thin sections, which are then stained and observed or analyzed under a microscope. Sample fixation is necessary to preserve the sample's morphology and structure; paraffin is used for fixation before sectioning. However, current techniques cannot effectively coat biological samples layer by layer with paraffin for sectional preparation. Summary of the Invention

[0003] To overcome the shortcomings of the prior art, the present invention provides a biochemical sample slicing device and slicing method, which has the advantage of being able to coat the biological sample layer by layer with wax, and then slice the biological sample layer by layer.

[0004] A biochemical sample slicing device includes a circular base, with a central frame fixed on both sides of the circular base. A rotating rod is hinged to each central frame, and a fork is fixed to the lower part of each rotating rod.

[0005] Each of the aforementioned frames is equipped with a motor, which drives the corresponding rotating rod to rotate.

[0006] The circular base is fixed in the middle of the horizontal column, and the upper parts of the two vertical rods are slidably connected to the left and right ends of the horizontal column, respectively. The vertical rods slide on the horizontal column through a telescopic rod.

[0007] The lower part of the vertical rod is rotatably connected to a rotating shaft, and clamps are fixed at the opposite ends of the two rotating shafts. Each clamp is provided with multiple protrusions.

[0008] A biochemical sample slicing method includes the following steps:

[0009] S1: Hold the biological sample between the two forks;

[0010] S2: Immerse the biological sample in wax using two forks;

[0011] S3: Drive the two clamps to grip the biological sample and release the two forks;

[0012] S4: Alternately clamp the two forks and two clamps onto the biological sample, coating the biological sample with wax layer by layer;

[0013] S5: Place the wax-coated biological sample on a disc, and use a cutter to slice the sample layer by layer by moving it left and right. Attached Figure Description

[0014] The present invention will now be described in further detail with reference to the accompanying drawings and specific implementation methods.

[0015] Figure 1 A flowchart of a biochemical sample slicing method;

[0016] Figure 2 A schematic diagram of the structure of a biochemical sample slicing device Figure 1 ;

[0017] Figure 3 A schematic diagram of the structure of a biochemical sample slicing device Figure 2 ;

[0018] Figure 4 A schematic diagram of the structure of a biochemical sample slicing device Figure 3 ;

[0019] Figure 5 Schematic diagram of the horizontal column structure Figure 1 ;

[0020] Figure 6 Schematic diagram of the horizontal column structure Figure 2 ;

[0021] Figure 7 Schematic diagram of the vertical rod Figure 1 ;

[0022] Figure 8 Schematic diagram of the vertical rod Figure 2 ;

[0023] Figure 9 This is a schematic diagram of the L-shaped rod.

[0024] Figure 10 Schematic diagram of the structure of the square box and the disc Figure 1 ;

[0025] Figure 11 Schematic diagram of the structure of the square box and the disc Figure 2 .

[0026] In the diagram: Horizontal column 101; Telescopic rod 102; Round seat 103; Middle frame 104; Motor 105; Rotating rod 106; Fork 107; Vertical column 108;

[0027] Vertical rod 201; Lifting column 202; Telescopic rod 203; convex seat 204; Rack 205; Gear 206; Rotating shaft 207; Clamp 208; Protrusion 209;

[0028] L-shaped bar 301; inclined plate 302;

[0029] Square box 401; heating wire 402; round base 403; arc-shaped plate 404; telescopic rod 405;

[0030] 501. Disc; 502. L-shaped part; 503. Vertical rail; 504. Telescopic rod; 505. Horizontal frame; 506. Support; 507. Vertical piece; 508. Cutter. Detailed Implementation

[0031] like Figure 5-6 As shown, this example demonstrates how wax can be wrapped around the outside of a biological sample.

[0032] The biochemical sample slicing device includes a circular base 103, with a central frame 104 welded to both sides of the circular base 103. Each central frame 104 is hinged with a rotating rod 106, and a fork 107 is welded to the lower part of each rotating rod 106. The two rotating rods 106 can rotate on the two central frames 104 respectively, thereby driving the two forks 107 to separate or close. Thus, the biological sample can be clamped by the two forks 107 while reducing the contact area with the biological sample. Then, the biological sample is immersed in wax, and the wax is wrapped around the outside of the biological sample.

[0033] like Figure 5-6 As shown, this example can achieve the effect of driving the two forks 107 to separate or close.

[0034] Since each of the middle frames 104 is connected to a motor 105 by screws, the motor 105 drives the corresponding rotating rod 106 to rotate. The motor 105 can drive the rotating rod 106 to rotate, thereby driving the two forks 107 to separate or close.

[0035] like Figure 5-8 As shown, this example demonstrates how to wrap a biological sample with multiple layers of wax.

[0036] Since the circular base 103 is welded to the middle of the horizontal column 101, the upper parts of the two vertical rods 201 are slidably connected to the left and right ends of the horizontal column 101, respectively. The vertical rods 201 are driven to slide on the horizontal column 101 by the telescopic rod 102. When the two forks 107 clamp the biological sample, they wrap the outside of the biological sample with wax. Then, the two vertical rods 201 are driven to move closer to each other, clamping the biological sample with the two vertical rods 201. Then, the two forks 107 are released, and the outside of the biological sample is wrapped with another layer of wax by the clamping of the two vertical rods 201, preventing the two forks 107 from sinking into the wax on the outside of the biological sample. Through the alternating clamping of the two vertical rods 201 and the two forks 107, the outside of the biological sample is wrapped with multiple layers of wax.

[0037] like Figure 7-8As shown, this example can achieve the effect of preventing slippage when holding a sample by means of the clamp 208 and the multiple protrusions 209 on the clamp 208.

[0038] The lower part of the vertical rod 201 is rotatably connected to the rotating shaft 207 via a bearing. The opposite ends of the two rotating shafts 207 are welded with clamps 208. Each clamp 208 is provided with multiple protrusions 209. The clamps 208 and the multiple protrusions 209 on the clamps 208 facilitate the clamping of the sample without slipping.

[0039] like Figure 7-8 As shown, this example demonstrates how to achieve the effect of easily and evenly adhering wax to the outside of a biological sample.

[0040] The driving lifting column 202 slides vertically on the boss 204, thereby driving the rack 205 to slide up and down, which in turn drives the gear 206, the rotating shaft 207 and the clamp 208 to rotate. When the two clamps 208 hold the biological sample, they are driven to rotate around the axis of the rotating shaft 207, thereby driving the biological sample to rotate in the wax, so that the wax can be evenly adhered to the outside of the biological sample.

[0041] like Figure 5-11 As shown, this example demonstrates how to control the immersion of biological samples in or removal from wax.

[0042] Since vertical columns 108 are welded to both ends of the horizontal column 101, the two vertical columns 108 are vertically slidably connected to both sides of the square box 401. The vertical columns 108 are driven to slide through the telescopic rod 405. The square box 401 is used to put molten wax. The two vertical columns 108 can slide vertically on the square box 401, thereby driving the horizontal column 101, the two forks 107 and the two clamps 208 to rise and fall, thereby controlling the biological sample to be immersed in the wax or to leave the wax. This continuous repetition can coat the outside of the biological sample with wax layer by layer.

[0043] like Figure 10-11 As shown, this example can achieve the effect of melting the wax placed inside the box 401.

[0044] Since the four corners of the square box 401 are connected to the round base 403 by screws, and the upper side of the round base 403 is welded with the arc-shaped piece 404, and the inner side of the arc-shaped piece 404 is fixed with the heating wire 402, the wax placed inside the square box 401 can be melted by the heating of the heating wire 402.

[0045] like Figure 5-9 As shown, this example can achieve the effect of wax flowing inside the square box 401 through four curved plates 404.

[0046] Because four L-shaped rods 301 are fixed in a ring on the circular seat 103, and inclined plates 302 are welded to the lower part of each of the four L-shaped rods 301, the four inclined plates 302 press on the upper part of the four arc-shaped pieces 404 respectively. When the horizontal column 101 and the circular seat 103 rise and fall, the biological sample is driven to rise and fall in the wax, and at the same time, the four L-shaped rods 301 and the four inclined plates 302 are driven to rise and fall. When the four inclined plates 302 rise and fall, they press the arc-shaped pieces 404, so that the four arc-shaped pieces 404 swing in the wax through elasticity, and then the wax flows in the square box 401 through the four arc-shaped pieces 404, making it easier for the wax to wrap around the outside of the biological sample.

[0047] like Figure 10-11 As shown, this example can achieve the effect of slicing a biological sample layer by layer.

[0048] A vertical rail 503 is provided in the middle of the rear side of the square box 401. An L-shaped part 502 is slidably connected to the vertical rail 503. A disc 501 is welded to the L-shaped part 502. The L-shaped part 502 is driven to slide by the telescopic rod 504. A bracket 506 is welded to the rear side of the square box 401. A horizontal frame 505 is slidably connected to the bracket 506. A vertical piece 507 is welded to the left end of the horizontal frame 505. A cutter 508 is integrally formed at the lower part of the vertical piece 507. The cutter 508 is located above the disc 501. The biological sample wrapped in wax can be placed on the disc 501. Then, the L-shaped part 502 and the disc 501 are driven to rise and fall to adjust the height. The horizontal frame 505 is manually driven to slide on the bracket 506, so that the vertical piece 507 drives the cutter 508 to cut the biological sample wrapped in wax, thereby slicing the biological sample layer by layer.

[0049] A biochemical sample slicing method includes the following steps:

[0050] S1: Clamp the biological sample between the two forks 107;

[0051] S2: Immerse the biological sample in wax using two forks 107;

[0052] S3: Drive the two clamps 208 to clamp onto the biological sample and release the two forks 107;

[0053] S4: Alternately clamp the two forks 107 and the two clamps 208 onto the biological sample, coating the biological sample with wax layer by layer;

[0054] S5: Place the wax-coated biological sample on the disc 501, and use the cutter 508 to move left and right to cut the wax-coated biological sample layer by layer.

Claims

1. A biochemical sample slicing device, comprising a circular base (103), characterized in that: Both sides of the circular base (103) are fixed with a middle frame (104), and each middle frame (104) is hinged with a rotating rod (106), and each rotating rod (106) is fixed with a fork (107) at its lower part. Each of the aforementioned intermediate frames (104) is fixed with a motor (105), and the motor (105) drives the corresponding rotating rod (106) to rotate; The circular seat (103) is fixed in the middle of the horizontal column (101), and the upper parts of the two vertical rods (201) are slidably connected to the left and right ends of the horizontal column (101), respectively. The vertical rods (201) are driven to slide on the horizontal column (101) by the telescopic rod (102). The lower part of the vertical rod (201) is rotatably connected to a rotating shaft (207), and the opposite ends of the two rotating shafts (207) are fixed with clamps (208), and each clamp (208) is provided with multiple protrusions (209). A gear (206) is fixed to the outer end of the rotating shaft (207), a boss (204) is fixed to the vertical rod (201), a lifting column (202) is vertically slidably connected to the boss (204), the lifting column (202) is driven to slide through the telescopic rod (203), and a rack (205) is fixed to the lower end of the lifting column (202), the rack (205) meshes with the gear (206) for transmission; The horizontal column (101) is fixed with vertical columns (108) at both ends. The two vertical columns (108) are vertically slidably connected to the two sides of the square box (401). The vertical columns (108) are driven to slide by the telescopic rod three (405). A round base (403) is fixed at each of the four corners inside the square box (401). An arc-shaped piece (404) is fixed on the upper side of the round base (403), and an electric heating wire (402) is fixed on the inner side of the arc-shaped piece (404). The circular base (103) has four L-shaped rods (301) fixed in a ring. The lower part of each of the four L-shaped rods (301) is fixed with an inclined plate (302). The four inclined plates (302) press on the upper part of the four arc-shaped pieces (404) respectively. A biochemical sample slicing method includes the following steps: S1: Hold the biological sample between the two forks (107); S2: Immerse the biological sample in wax using two forks (107); S3: Drive the two clamps (208) to clamp onto the biological sample and release the two forks (107); S4: Alternately clamp the two forks (107) and the two clamps (208) onto the biological sample to coat the biological sample with wax layer by layer; S5: Place the wax-coated biological sample on the disc (501), and cut the wax-coated biological sample layer by layer by moving the cutter (508) left and right.

2. The biochemical sample slicing device according to claim 1, characterized in that: A vertical rail (503) is provided in the middle of the rear side of the square box (401). An L-shaped piece (502) is slidably connected to the vertical rail (503). A disc (501) is fixed on the L-shaped piece (502). The L-shaped piece (502) is driven to slide by the telescopic rod (504). A bracket (506) is fixed on the rear side of the square box (401). A horizontal frame (505) is slidably connected on the bracket (506). A vertical piece (507) is fixed at the left end of the horizontal frame (505). A cutter (508) is provided at the lower part of the vertical piece (507). The cutter (508) is located above the disc (501).

Images