A fully automated intelligent robotic workstation integrating nucleic acid sampling, detection and analysis

Abstract

This invention discloses a fully automated intelligent robotic workstation for nucleic acid sampling, detection, and analysis. It comprises a movable prefabricated building with multiple unit compartments arranged sequentially from left to right. These unit compartments, from left to right, are a sampling room, a sample tube loading room, a specimen preparation room, and a detection and specimen processing room. A transfer mechanism is provided between adjacent unit compartments. The sample tube loading room, specimen preparation room, and detection and specimen processing room are all enclosed spaces. Each unit compartment has a corresponding transfer hole on its wall for the transfer mechanism. The transfer mechanism includes a conveyor belt located at the bottom of the transfer hole and extending laterally, and an airtight door located within the transfer hole that divides the conveyor belt into two sections. This system can automatically collect nucleic acid samples and directly perform nucleic acid detection and analysis without needing to transport them to other locations, thus eliminating the risk of infection during transport. It can also receive nucleic acid samples collected from other locations for testing.

Description

Technical Field

[0001] This invention relates to the field of medical testing equipment technology, specifically to a fully automated intelligent robot workstation integrating nucleic acid sampling, testing and analysis. Background Technology

[0002] Conducting nucleic acid testing is of great practical and far-reaching historical significance for early detection, identifying targets, determining directions, responding effectively, ensuring safety, resuming work and production, restoring the economy, and promoting economic and social development.

[0003] However, the existing sample collection and testing process has many problems, such as dense crowds, cumbersome procedures, inadequate protection, separation of links, and time delays. In particular, the most commonly used methods, such as throat swab sampling, have problems such as high risk of cross-infection during the collection process, inconsistent collection methods due to human factors, and problems with the delivery and storage of collected samples, which greatly affect the safety and efficiency of virus testing. Summary of the Invention

[0004] This invention aims to solve the technical problems existing in the prior art, and innovatively proposes a fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation, which can collect nucleic acid on its own and directly perform nucleic acid detection and analysis without the need to transport it to other places, thus avoiding the infection situation during the transportation process. At the same time, it can also receive nucleic acid samples collected from other places for testing.

[0005] To achieve the above-mentioned objectives of the present invention, the present invention provides a fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation, comprising a movable prefabricated house with multiple unit compartments arranged sequentially from left to right. The unit compartments, from left to right, are a sampling room, a sampling tube loading room, a specimen preparation room, and a detection and specimen processing room. A transfer mechanism is provided between two adjacent unit compartments. The sampling tube loading room, specimen preparation room, and detection and specimen processing room are all sealed spaces. Each unit compartment has a partition wall transfer hole on the wall corresponding to the transfer mechanism. The transfer mechanism includes a conveyor belt located at the bottom of the partition wall transfer hole and extending to the left and right, and an airtight door set in the partition wall transfer hole and dividing the conveyor belt into two sections.

[0006] Photoelectric sensor 1 and photoelectric sensor 2 are fixed by brackets next to the conveyor belt. Photoelectric sensor 1 and photoelectric sensor 2 are respectively set on both sides of the wall and are used to automatically identify whether there is material at the material start position and material end position of the two-section conveyor belt, and are linked with the two-section conveyor belt action mechanism.

[0007] The sampling room is equipped with an automatic coding machine for affixing information of the person to be tested onto the sampling tubes and a nucleic acid sampling robot with facial recognition function. The nucleic acid sampling robot is enclosed by a partition wall in the right corner of the sampling room, thus separating it from other areas. This area is the sampling area, and the other areas are the sampling tube preparation areas. The sampling tube preparation area has an entrance door on the wall of the prefabricated building. A sampling tube rack feeding module for transporting sampling tubes is connected between the sampling tube preparation area and the sampling area. A sampling window is opened on the wall of the prefabricated building in the sampling area. The sampling window is also equipped with an intelligent all-in-one machine for human-computer interaction. The intelligent all-in-one machine is electrically connected to an intelligent server. The data transmission terminal of the automatic coding machine for the sampling tubes is connected to the data transmission terminal of the intelligent all-in-one machine.

[0008] The sampling tube loading chamber is equipped with a transmission mechanism for connecting the transfer mechanisms on the left and right sides. The sample transfer window, which is normally closed, is opened on the prefabricated wall of the sampling tube loading chamber. Nucleic acid samples collected elsewhere are placed on the transmission mechanism through the sample transfer window.

[0009] In the above scheme: the specimen preparation chamber is equipped with a nucleic acid extractor loading / unloading arm and a nucleic acid extractor and a pipetting / sealing device arranged around the nucleic acid extractor loading / unloading arm. The specimen extracted by the nucleic acid extractor, the separated waste liquid and the waste from the sampling tube are moved to the transfer mechanism between the specimen preparation chamber and the detection and specimen processing chamber through the nucleic acid extractor loading / unloading arm.

[0010] The testing and specimen processing room is equipped with a PCR loading / unloading arm and a PCR detector, specimen storage rack, and autoclave arranged around the PCR loading / unloading arm. The PCR detector's test result data sending end is connected to the intelligent all-in-one machine's test result data receiving end. The right side wall of the testing and specimen processing room has an installation port for embedding the autoclave, and the autoclave's outlet is located outside the movable prefabricated room. A sterilization item transfer module is connected between the specimen storage rack and the autoclave.

[0011] The sampling tube preparation area is equipped with a sampling tube rack support and a sampling tube rack loading arm. The sampling tube rack is placed on the sampling tube rack feeding module through the sampling tube rack loading arm. The transfer mechanism between the sampling area and the sampling tube loading chamber is the sampling tube rack discharging module. A sampling conveyor belt connects the sampling tube rack discharging module and the sampling tube rack feeding module.

[0012] The transfer mechanism between the sampling tube loading chamber and the specimen preparation chamber is a sample tube rack dispensing module. The sampling tube loading chamber is equipped with an external sampling tube rack corresponding to the external sample transfer window. An external sampling tube rack conveyor belt connects the external sampling tube rack and the sample tube rack dispensing module.

[0013] In the above scheme: the specimen preparation room is also connected to the consumables and equipment preparation room, the consumables and equipment preparation room is equipped with a consumables bracket and a consumables picking arm, and the consumables and equipment preparation room is connected to the specimen preparation room by a consumables feeding module, and the consumables on the consumables bracket are placed on the consumables feeding module by the consumables picking arm;

[0014] The specimen preparation chamber is equipped with a feeding conveyor belt for both the consumable feeding module and the sample tube rack dispensing module. The transfer mechanism between the specimen preparation chamber and the testing and specimen processing chamber includes a PCR feeding module, a waste transfer module, and a sampling tube waste transfer module. The dispensing end of the pipetting / sealing device is connected to the PCR feeding module. The ends of the PCR feeding module and the sampling tube waste transfer module extend into the testing and specimen processing chamber. The sealed samples and sampling tube waste are respectively sent to the testing and specimen processing chamber for processing through the PCR feeding module and the sampling tube waste transfer module.

[0015] In the above scheme: the sampling tube loading room and the consumables and equipment preparation room are located in the same unit compartment and are adjacent to each other. The sampling area is also located in front of the sampling room, making reasonable use of space.

[0016] In the above scheme, the walls of the prefabricated houses for the sampling area, the sampling tube loading room, the specimen preparation room, and the testing and specimen processing room are all equipped with inspection ports to facilitate staff to enter the rooms for maintenance.

[0017] In the above scheme: an active sunshade device is provided outside the sampling window to facilitate people undergoing nucleic acid testing to avoid rain and sun.

[0018] In the above scheme: an equipment room is also provided on the left side of the sampling room, the intelligent server is set in the equipment room, each unit compartment is equipped with monitoring equipment, and the monitoring data of all monitoring equipment is transmitted to the equipment room, which is equipped with an entrance door.

[0019] In the above scheme: ultraviolet sterilization lamps are installed on the upper, left and right sides of both ends of the partition wall transmission hole, and an air curtain machine is installed above the high-pressure end of the partition wall transmission hole, i.e., the side where the material starts; the air curtain machine is used for air isolation, and plays a double isolation role on the basis of the isolation between the high-pressure room and the low-pressure room.

[0020] In the above scheme: the conveyor belt is a two-section conveyor belt, which is respectively set on both sides of the wall;

[0021] The conveyor belt includes: a two-section conveyor belt actuation mechanism and a two-section conveyor belt support, which are disposed below the two-section conveyor belt.

[0022] In the above scheme: the airtight door is equipped with a limit switch to protect the safety of the airtight door motor; both the airtight door and the two-section conveyor belt are equipped with control buttons that can force changes to the working state.

[0023] In summary, due to the adoption of the above technical solutions, the beneficial effects of this invention are as follows: the nucleic acid sampling robot and intelligent all-in-one machine can be used for self-service sampling, and the nucleic acid sampling robot with facial recognition function avoids false detection; the normally closed transfer door and the sealed sampling area, sampling tube loading room, specimen preparation room and testing and specimen processing room can ensure that the nucleic acid collection, extraction and testing system adopts a fully automated and fully enclosed "sample in, result out" form as much as possible, minimizing operational errors and reducing the risk of personnel infection; there is no need to transfer to other testing institutions, and testing can be carried out immediately after collection, improving testing efficiency; after testing, sterilization and disinfection are carried out immediately, and the safety performance is no less than that of routine clinical nucleic acid testing and POCT closed testing, preventing contamination and having low or no biosafety hazards. Attached Figure Description

[0024] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0025] Figure 1 This is a schematic diagram of the prefabricated house of the present invention;

[0026] Figure 2 This is a schematic diagram of the structure of the present invention;

[0027] Figure 3 This is a schematic diagram of the transmission mechanism of the present invention.

[0028] Figure 4 This is a schematic diagram of the front view structure of the transmission mechanism of the present invention.

[0029] Figure 5 This is a flowchart illustrating the operation of the transfer mechanism of the present invention.

[0030] Figure 6 This is a schematic diagram of the control principle of the two-section conveyor belt section of the present invention.

[0031] Figure 7 This is a schematic diagram of the airtight door control principle of the present invention.

[0032] Figure 8 This is a schematic diagram of the control principle of the air curtain machine of the present invention. Detailed Implementation

[0033] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0034] like Figures 1-2 As shown, a fully automated intelligent robotic nucleic acid sampling and analysis integrated workstation includes a prefabricated building with multiple unit compartments arranged from left to right. The unit compartments, from left to right, are a sampling room, a sampling tube loading room S7, a specimen preparation room S4, and a detection and specimen processing room S5. Each adjacent unit compartment is equipped with a transfer mechanism that allows nucleic acid samples to be transferred only from the left unit compartment to the right unit compartment. The sampling tube loading room S7, specimen preparation room S4, and detection and specimen processing room S5 are all enclosed spaces. To ensure stable transfer of nucleic acid samples, the walls shared by adjacent unit compartments are equipped with partition wall transfer holes A10 corresponding to the transfer mechanisms. The transfer mechanism includes a conveyor belt located at the bottom of the partition wall transfer hole A10 and extending laterally, and an airtight door A1 located within the partition wall transfer hole A10, dividing the conveyor belt into two sections A5; this door is only opened during nucleic acid sample transfer to reduce airflow between unit compartments and prevent cross-infection. To further prevent cross-infection, ultraviolet sterilization lamps A4 are installed on the upper, left, and right sides of both ends of the partition wall transfer hole A10, and an air curtain machine A3 is installed above the high-pressure end of the partition wall transfer hole A10. The airtight door A1 is equipped with a limit switch to protect the safety of the airtight door A1 motor, and the connection between the airtight door A1 and the partition wall transfer hole A10 is made of polyurethane rubber material.

[0035] Photoelectric sensor A1A8 and photoelectric sensor A2A11 are fixed to the side of the conveyor belt via bracket A9. Photoelectric sensor A1A8 and photoelectric sensor A2A11 are respectively set on both sides of the wall and are used to automatically identify whether there is material on the material start position A6 and material end position A12 of the two-section conveyor belt A5, and are linked with the two-section conveyor belt action mechanism A7.

[0036] The conveyor belt is a two-section conveyor belt A5, which is set on both sides of the wall. The conveyor belt includes: a two-section conveyor belt actuation mechanism A7 and a two-section conveyor belt support A13 set below the two-section conveyor belt A5.

[0037] The sampling room is equipped with a nucleic acid sampling robot 4 with facial recognition capabilities. The robot 4 is enclosed by a partition in the front right corner of the sampling room, separating it from other areas. This area is designated as sampling area S6, while the other area is the sampling tube preparation area S2. An entrance door J1 is located on the wall of the sampling tube preparation area S2. A sampling tube rack feeding module 24 connects sampling tube preparation area S2 and sampling area S6, allowing medical staff to manually prepare sampling tubes in sampling tube preparation area S2 and then pass them to sampling area S6 to replenish collection consumables. Correspondingly, both ends of the sampling tube rack feeding module 24 are also equipped with transfer doors.

[0038] Ideally, the sampling tube preparation area S2 is also equipped with a sampling tube rack support 23 and a sampling tube rack loading arm 25. The sampling tube rack 26 is automatically placed on the sampling tube rack feeding module 24 by the sampling tube rack loading arm 25, which improves the degree of automation, further reduces manual input, and improves the protection effect.

[0039] A sampling window is located at the front of sampling area S6, and a smart all-in-one machine 3 for human-computer interaction is installed on the sampling window. The smart all-in-one machine 3 is electrically connected to a smart server 1. Sampling area S6 also contains an automatic sampling tube coding machine 2. The data transmission end of the automatic sampling tube coding machine 2 is connected to the data transmission end of the smart all-in-one machine 3. The automatic sampling tube coding machine 2 is used to affix the information of the person to be inspected obtained from the smart all-in-one machine 3 to the sampling tube. The automatic sampling tube coding machine 2 is equipped with a storage box for storing the sampling tubes.

[0040] The transfer mechanism between the sampling area S6 and the sampling tube loading chamber S7 is the sampling tube rack discharge module 5, and a sampling conveyor belt connects the sampling tube rack discharge module 5 and the sampling tube rack feeding module 24.

[0041] During nucleic acid collection, the nucleic acid sampling robot 4 removes the sampling tube with the information pasted on the automatic coding machine 2 before collecting the nucleic acid. After the nucleic acid collection is completed, the sampling tube is placed on the sampling tube rack 26 and conveyed to the sampling tube loading chamber S7 via the sampling tube rack discharge module 5.

[0042] The sampling tube loading chamber S7 is equipped with a transmission mechanism connecting the transfer mechanisms on the left and right sides. A normally closed external sample transfer window J5 is located on the prefabricated wall of the sampling tube loading chamber S7. Nucleic acid samples collected elsewhere are placed onto the transmission mechanism through the external sample transfer window J5. The transfer mechanism between the sampling tube loading chamber S7 and the specimen preparation chamber S4 is the sample tray discharge module 8. An external sampling tray 6 is located in the sampling tube loading chamber S7 corresponding to the external sample transfer window J5. An external sampling tray conveyor belt 7 connects the external sampling tray 6 and the sample tray discharge module 8.

[0043] The specimen preparation room S4 is also connected to the consumables and equipment preparation room S3. The consumables and equipment preparation room S3 is equipped with a consumables support 21 and a consumables picking arm 22. The consumables and equipment preparation room S3 and the specimen preparation room S4 are connected by a consumables feeding module 20. The consumables on the consumables support 21 are automatically placed on the consumables feeding module 20 by the consumables picking arm 22, so as to replenish the consumables to the specimen preparation room S4 in a timely manner.

[0044] The sampling tube loading chamber S7 and the consumables and equipment preparation chamber S3 are located in the same unit compartment and are adjacent to each other. The sampling area S6 is also located in front of the sampling chamber, making reasonable use of the space.

[0045] Both the consumable feeding module 20 and the sample tube rack dispensing module 8 in the specimen preparation chamber S4 are equipped with feeding conveyor belts. A nucleic acid extractor upper / lower arm 10 is centrally located in the specimen preparation chamber S4, and a nucleic acid extractor 9 is located to the left of the upper / lower arm 10. Consumables and sample tubes from the feeding conveyor belt are placed into the nucleic acid extractor 9 via the upper / lower arm 10 to extract nucleic acid samples.

[0046] A pipetting / sealing device 19 is located on the right side of the nucleic acid extractor's loading / unloading arm 10. The transfer mechanism between the sample preparation chamber S4 and the detection and sample processing chamber S5 includes a PCR feeding module 18, a waste transfer module 11, and a sampling tube waste transfer module 12. The discharge end of the pipetting / sealing device 19 is connected to the PCR feeding module 18. The ends of the PCR feeding module 18 and the sampling tube waste transfer module 12 both extend into the detection and sample processing chamber S5. After the nucleic acid extractor 9 extracts the sample, the sample, the separated waste liquid, and the sampling tube waste are moved to the pipetting / sealing device 19, the waste transfer module 11, and the sampling tube waste transfer module 12 respectively via the nucleic acid extractor's loading / unloading arm 10. After the sample is sealed, the sealed sample and the sampling tube waste are sent to the detection and sample processing chamber S5 for processing via the PCR feeding module 18 and the sampling tube waste transfer module 12 respectively.

[0047] The testing and specimen processing room S5 is equipped with a PCR loading / unloading arm 14 and a PCR detector 13, a specimen storage rack 17, and an autoclave 16 arranged around the PCR loading / unloading arm 14. The PCR detector 13's test result data sending end is connected to the intelligent all-in-one machine 3's test result data receiving end. The right side of the prefabricated wall of the testing and specimen processing room S5 has an installation port for the autoclave 16 to be embedded and installed, and the exhaust port of the autoclave 16 is located outside the prefabricated room. A sterilization item transfer module 15 is connected between the specimen storage rack 17 and the autoclave 16.

[0048] To ensure stable equipment operation, it is best to have inspection ports J4 on the walls of the prefabricated houses in the sampling area S6, the sampling tube loading room S7, the specimen preparation room S4, and the testing and specimen processing room S5, so that staff can enter the rooms regularly for maintenance.

[0049] Ideally, a movable sunshade device J2 should be installed outside the sampling window to provide shade from rain and sun for people undergoing nucleic acid testing.

[0050] The sampling room is located on the left side of the equipment room S1. The intelligent server is located in the equipment room S1. Each unit compartment is equipped with a monitoring device 27. All monitoring data from the monitoring devices 27 are transmitted to the equipment room S1. The equipment room S1 is equipped with an entrance door.

[0051] Before use, the sampling tube rack 26 is placed in the designated position on the sampling tube rack support 23 by hand, and the empty sampling tubes are placed in the storage box in the automatic sampling tube coding machine 2. At the same time, the consumables are placed in the designated position on the consumables support 21.

[0052] Upon startup, the sampling tube rack loading arm 25 automatically delivers the sampling tube rack 26 to the sampling tube rack feeding module 24 according to the set program. The sampling tube rack feeding module 24 then automatically delivers the sampling tube rack 26 to the designated position at the nucleic acid sampling robot 4. After registering their information on the intelligent all-in-one machine 3, the person being tested moves to the nucleic acid sampling window. The automatic sampling tube coding machine 2 automatically affixes an identification code to the empty sampling tube based on the person's registration information and then automatically delivers it to the nucleic acid sampling robot 4 for later use. The nucleic acid sampling robot 4 automatically and accurately collects the nucleic acid sample from the person being tested using the facial recognition function on its arm and stores it in the empty sampling tube delivered by the automatic sampling tube coding machine 2, which is then placed sequentially onto the sampling tube rack 26.

[0053] The nucleic acid sampling robot 4 is equipped with both single and mixed testing functions. After the sampling tube rack 26 at the nucleic acid sampling robot 4 is full, the sampling tube rack feeding module 24 sends the sampling tube rack 26 to the sampling tube rack discharging module 5.

[0054] If there is an external sampling tube rack 6, it is placed sequentially on the sampling tube rack transfer device 7 through the external sample transfer window J5. The sampling tube rack discharge module 5 is linked with the sampling tube rack transfer device 7. The sampling tube rack transfer device 7 automatically sends the sampling tube rack 26 and the external sampling tube rack 6 sequentially to the sampling tube rack discharge module 8 to be tested, and then they are transferred to the side of the nucleic acid extractor 9 for testing.

[0055] The consumables picking arm 22 automatically places the consumables in the consumables holder 21 into the consumables feeding module 20 and then transfers them to the side of the nucleic acid extractor 9 for later use. The nucleic acid extractor loading / unloading arm 10 sequentially puts the sampling tube rack 26 / external sampling tube rack 6 stored on the sampling tube rack dispensing module 8 and the consumables on the consumables feeding module 20 into the nucleic acid extractor 9 for nucleic acid extraction.

[0056] After the nucleic acid extractor 9 completes its operation, the nucleic acid extractor loading / unloading arm 10 places the nucleic acid extractant from the extractor 9 onto the pipetting / sealing device 19, transfers the sampling tube waste to the sampling tube waste transfer module 12, and transfers the remaining materials to the waste transfer module 11. The sampling tube waste is then transferred by the sampling tube waste transfer module 12 to the testing and specimen processing room S5 and transferred by the PCR loading / unloading arm 14 to the specimen storage rack 17 for temporary storage. After the temporary storage time for the sampling tube waste is over, the PCR loading / unloading arm 14 places it on the sterilization item transfer module 15 and sends it to the autoclave 16 for sterilization. The waste transfer module 11 sends the materials used by the nucleic acid extractor 9 to the testing and specimen processing room S5, where they are packaged and then transferred by the PCR loading / unloading arm 14 to the sterilization item transfer module 15 and sent to the autoclave 16 for sterilization.

[0057] The pipetting / sealing device 19 processes the delivered nucleic acid extract into PCR plates, which are then delivered to the PCR detector 13 via the PCR feeding module 18 for testing. The PCR loading / unloading arm 14 automatically delivers the PCR plates from the PCR feeding module 18 into the PCR detector 13 for nucleic acid detection. After testing, the PCR loading / unloading arm 14 automatically transfers the PCR plates from the PCR detector 13 to the specimen storage rack 17 for temporary storage. After the temporary storage time is up, the PCR loading / unloading arm 14 places the PCR plates onto the sterilization item transfer module 15 and delivers them to the autoclave 16 for sterilization.

[0058] After sterilization, the materials are automatically discharged outdoors by the autoclave 16 and promptly sorted and disposed of by staff. The test results of the PCR testing instrument 13 are uploaded to professionals for analysis, and the analysis results are posted online and can be printed by the person being tested on the intelligent all-in-one machine 3.

[0059] Staff members maintain and repair the equipment in the sampling area S6, specimen preparation room S4, and testing and specimen processing room S5 through the glass windows of the sampling room and inspection ports J3 and J4, respectively, to ensure the stable operation of the equipment.

[0060] This invention also includes an operation control method for an automated material transfer system in a biosafety laboratory, comprising the following steps:

[0061] S1, system in operation;

[0062] S2, UV sterilization lamp; A4 normally open air curtain machine; A3 closed airtight door; A1 closed two-section conveyor belt; A5 closed.

[0063] S3: Determine if there is material at the material start position A6. If there is material, proceed to the next step; if there is no material, proceed to step S3 again.

[0064] S4, the two-section conveyor belt A5 is activated;

[0065] S5, Air curtain machine A3 is turned on;

[0066] S6, airtight door A1 opens;

[0067] S7, determine whether there is material at the material endpoint A12. If there is material, proceed to the next step; if there is no material, proceed to step S7 again.

[0068] S8, two-section conveyor belt A5 closed.

[0069] S9, airtight door A1 closed.

[0070] S10, Air curtain machine A3 closed.

[0071] S11, a single material transfer is completed, proceed to step S3.

[0072] like Figures 4-6 As shown, the operating control principle of an automated material transfer system for a biosafety laboratory is as follows:

[0073] Figure 4 The control principle diagram for the two-section conveyor belt of this invention is as follows: When photoelectric sensor A8 detects material information, the normally open contact 1k automatically closes, contactor KM1 is energized, and the normally open contact of KM1 automatically closes. When photoelectric sensor A8 detects material information, the two-section conveyor belt operating mechanism A7 operates, and the two-section conveyor belt A5 begins to transport material; simultaneously, contactors KM2 and KM3 are energized, the normally open contact of KM2 automatically closes, the normally open contact of KM3 automatically closes, and the normally closed contact automatically opens.

[0074] When photoelectric sensor A11 detects material information, normally closed contact 1k1 automatically opens, contactor KM1 is de-energized, and KM1 contacts return to their original state. When photoelectric sensor A11 detects material information, the two-section conveyor belt operating mechanism A7 stops operating, and the two-section conveyor belt A5 stops; simultaneously, contactors KM2 and KM3 are de-energized, and KM2 and KM3 contacts return to their original state.

[0075] ZK is the main switch, and SB1 is the start switch for the two-stage conveyor belt in manual mode.

[0076] Figure 7This is a schematic diagram of the airtight door control principle of the present invention: When there is no external signal, i.e., when the photoelectric sensor does not detect any material, contactor KM5 is energized, normally closed double-pole switch KM5 is in the closed state, the airtight door A1 motor reverses operation, and airtight door A1 begins to move downwards to close. When airtight door A1 reaches the position of limit switch contact SQ2, limit switch contact SQ2 opens, contactor KM5 is de-energized, normally open contact of KM5 automatically closes, normally closed contact automatically opens; normally closed double-pole switch KM5 becomes open, and airtight door A1 motor stops moving.

[0077] When the photoelectric sensor A8 detects material information Figure 4 When contactor KM3 is energized, its normally open contact automatically closes and its normally closed contact automatically opens. When contactor KM4 is energized, the normally open double-pole switch KM4 closes, the airtight door A1 motor rotates forward, and the airtight door A1 begins to move upward and open. When the airtight door A1 reaches the position of the limit switch contact SQ1, the limit switch contact SQ1 opens, contactor KM4 is de-energized, its normally open contact automatically closes and its normally closed contact automatically opens, and the airtight door A1 motor stops moving.

[0078] In addition, control buttons SBS3 and SBS4 are provided to forcefully control the opening and closing of the airtight door A1. When control button SBS3 is pressed, the airtight door A1 changes from forward to reverse; when control button SBS4 is pressed, the airtight door A1 changes from reverse to forward. SBS2 is an emergency stop switch.

[0079] When photoelectric sensor 2A11 detects material information Figure 3 When contactor KM3 is de-energized, its normally open contact is open and its normally closed contact is closed; when contactor KM5 is energized, the normally closed double-pole switch KM5 closes, the airtight door A1 motor reverses, and the airtight door A1 closes. When the airtight door A1 reaches the limit switch contact SQ2, the limit switch contact SQ2 opens, contactor KM5 is de-energized, its normally open contact automatically closes, and its normally closed contact automatically opens; the normally closed double-pole switch KM5 becomes open, and the airtight door A1 motor stops moving.

[0080] Figure 8 This is a schematic diagram of the air curtain machine control principle of the present invention: When Figure 3 When contactor KM2 is energized, its normally open contact automatically closes, energizing the power supply circuit for air curtain machine A3 and starting air curtain machine A3. When contactor KM2 is de-energized, its contacts return to their original state, de-energizing the power supply circuit for air curtain machine A3 and shutting down air curtain machine A3. Circuit breakers are always connected first.

[0081] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A fully automated intelligent robotic workstation integrating nucleic acid sampling, detection, and analysis, characterized in that: The prefabricated house includes multiple unit compartments arranged from left to right. The unit compartments are, from left to right, a sampling room, a sampling tube loading room (S7), a specimen preparation room (S4), and a testing and specimen processing room (S5). A transfer mechanism is provided between two adjacent unit compartments. The sampling tube loading room (S7), the specimen preparation room (S4), and the testing and specimen processing room (S5) are all enclosed spaces. Each unit compartment has a partition wall transfer hole (A10) on the wall corresponding to the transfer mechanism. The transfer mechanism includes a conveyor belt located at the bottom of the partition wall transfer hole (A10) and extending to the left and right, and an airtight door (A1) set in the partition wall transfer hole (A10) that divides the conveyor belt into two sections (A5). Photoelectric sensor 1 (A8) and photoelectric sensor 2 (A11) are fixed by bracket (A9) next to the conveyor belt. Photoelectric sensor 1 (A8) and photoelectric sensor 2 (A11) are respectively set on both sides of the wall and are used to automatically identify whether there is material at the material start position (A6) and material end position (A12) of the two-section conveyor belt (A5) and are linked with the two-section conveyor belt action mechanism (A7). The sampling room is equipped with an automatic coding machine (2) for attaching information of the person to be tested to the sampling tube and a nucleic acid sampling robot (4) with face recognition function. The nucleic acid sampling robot (4) is enclosed in the right corner of the sampling room by an isolation partition, so as to separate it from other areas. This area is the sampling area (S6), and the other areas are the sampling tube preparation area (S2). The sampling tube preparation area (S2) has an entrance door (J1) on the wall of the prefabricated house. The sampling tube preparation area (S2) and the sampling area (S6) are connected by a sampling tube rack feeding module (24) for transmitting sampling tubes. The sampling area (S6) has a sampling window on the wall of the prefabricated house. The sampling window is also equipped with an intelligent all-in-one machine (3) for human-computer interaction. The intelligent all-in-one machine (3) is electrically connected to an intelligent server (1). In the sampling area (S6), the data transmission end of the automatic coding machine (2) is connected to the data transmission end of the intelligent all-in-one machine (3). The sampling tube loading chamber (S7) is equipped with a transmission mechanism for connecting the transfer mechanisms on the left and right sides. The sample transfer window (J5) in the prefabricated wall of the sampling tube loading chamber (S7) is normally closed. Nucleic acid samples collected elsewhere are placed on the transmission mechanism through the sample transfer window (J5). Ultraviolet sterilization lamps (A4) are installed on the upper, left and right sides of both ends of the partition wall transmission hole (A10), and an air curtain machine (A3) is installed above the high air pressure end of the partition wall transmission hole (A10). The conveyor belt is a two-section conveyor belt (A5), which is respectively installed on both sides of the wall; The conveyor belt includes: a two-section conveyor belt actuation mechanism (A7) and a two-section conveyor belt support (A13) disposed below the two-section conveyor belt (A5); The airtight door (A1) is equipped with a limit switch to protect the safety of the airtight door (A1) motor; The two-section conveyor belt (A5) includes the conveyor belt control circuitry: The first end of switch ZK is connected to the live wire L. The second end of switch ZK is connected to the first end of normally open push button switch SB1, the first end of normally open contact 1k of photoelectric sensor 1 (A8), and the first end of normally open contact of contactor KM1. The second end of push button switch SB1, the second end of normally open contact 1k of photoelectric sensor 1 (A8), and the second end of normally open contact of contactor KM1 are connected to the first end of normally closed contact 1k1 of photoelectric sensor 2 (A11). The second end of the normally closed contact 1k1 of photoelectric sensor 2 (A11) is connected to the first end of the normally closed push-button switch SBS1. The second end of the normally closed push-button switch SBS1 is connected to the first end of the winding of contactor KM1 and the first end of the normally open contact of contactor KM1. The second end of the normally open contact of contactor KM1 is connected to the first end of the winding of contactor KM2 and the first end of the winding of contactor KM3. The second ends of the windings of contactor KM1, KM2, and KM3 are connected to the neutral line N. The airtight door (A1) includes an airtight door control circuit: The first terminal of the main live wire switch LK is connected to the live wire L. The second terminal of the main live wire switch LK is connected to the first terminal of the emergency stop switch SBS2. The second terminal of the emergency stop switch SBS2 is connected to the first terminal of the normally open contact of contactor KM4, the first terminal of the normally open contact of contactor KM3, the first terminal of the normally open contact SBS41 of control button SBS4, the first terminal of the normally open contact SBS31 of control button SBS3, the first terminal of the normally closed contact of contactor KM3, and the first terminal of the normally open contact of contactor KM5. The second terminal of the normally open contact of contactor KM3 is connected to the first terminal of the normally open contact of contactor KM5. The second end of the normally open contact of contactor KM4, the second end of the normally open contact of contactor KM5, and the second end of the normally open contact SBS41 of control button SBS4 are connected to the first end of control button SBS3. The second end of control button SBS3 is connected to the first end of normally closed limit switch SQ1, and the second end of normally closed limit switch SQ1 is connected to the first end of the winding of contactor KM4. The second terminal of the normally open contact of control button SBS31, the second terminal of the normally closed contact of contactor KM3, and the second terminal of the normally open contact of contactor KM5 are connected to the first terminal of control button SBS4. The second terminal of control button SBS4 is connected to the first terminal of normally closed limit switch contact SQ2. The second terminal of normally closed limit switch contact SQ2 is connected to the first terminal of normally closed contact of contactor KM4. The second terminal of normally closed contact of contactor KM4 is connected to the first terminal of winding of contactor KM5. The second end of the contactor KM4 winding and the second end of the contactor KM5 winding are connected to the second end of the neutral line main switch NK, and the first end of the neutral line main switch NK is connected to the neutral line N. The first terminal of the L pole of the normally open double-pole contactor KM4 is connected to the second terminal of the main live wire switch LK. The first terminal of the N pole of the normally open double-pole contactor KM4 is connected to the second terminal of the main neutral wire switch NK. The second terminal of the L pole of the normally open double-pole contactor KM4 is connected to the first terminal of the capacitor and the first terminal of the airtight door (A1) motor. The second terminal of the capacitor is connected to the second terminal of the airtight door (A1) motor and the first terminal of the L pole of the normally closed double-pole contactor KM5. The third terminal of the airtight door (A1) motor is connected to the second terminal of the N pole of the normally open double-pole contactor KM4 and the first terminal of the N pole of the normally closed double-pole contactor KM5. The second terminal of the L pole of the normally closed double-pole contactor KM5 is connected to the second terminal of the main live wire switch LK. The second terminal of the N pole of the normally closed double-pole contactor KM5 is connected to the second terminal of the main neutral wire switch NK.

2. The fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation according to claim 1, characterized in that: The specimen preparation chamber (S4) is equipped with a nucleic acid extractor loading / unloading arm (10) and a nucleic acid extractor (9) and a pipetting / sealing device (19) arranged around the nucleic acid extractor loading / unloading arm (10). The specimen extracted by the nucleic acid extractor (9), the separated waste liquid and the waste from the sampling tube are moved to the transfer mechanism between the specimen preparation chamber (S4) and the detection and specimen processing chamber (S5) through the nucleic acid extractor loading / unloading arm (10). The testing and specimen processing room (S5) is equipped with a PCR loading / unloading arm (14) and a PCR detector (13), a specimen storage rack (17), and an autoclave (16) arranged around the PCR loading / unloading arm (14). The PCR detector (13) is connected to the intelligent all-in-one machine (3) for receiving test results. An installation port for the autoclave (16) is provided on the right side wall of the testing and specimen processing room (S5), and the outlet of the autoclave (16) is located outside the mobile prefabricated house. A sterilization item transfer module (15) is connected between the specimen storage rack (17) and the autoclave (16). The sampling tube preparation area (S2) is equipped with a sampling tube rack support (23) and a sampling tube rack loading arm (25). The sampling tube rack (26) is placed on the sampling tube rack feeding module (24) through the sampling tube rack loading arm (25). The transfer mechanism between the sampling area (S6) and the sampling tube loading chamber (S7) is the sampling tube rack discharge module (5). A sampling conveyor belt is connected between the sampling tube rack discharge module (5) and the sampling tube rack feeding module (24). The transfer mechanism between the sampling tube loading chamber (S7) and the specimen preparation chamber (S4) is the sample tube rack dispensing module (8). The sampling tube loading chamber (S7) is equipped with an external sample rack (6) corresponding to the external sample transfer window (J5). An external sample rack conveyor belt (7) connects the external sample rack (6) and the sample tube rack dispensing module (8).

3. The fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation according to claim 1, characterized in that: The specimen preparation chamber (S4) is also connected to the consumables and equipment preparation chamber (S3). The consumables and equipment preparation chamber (S3) is equipped with a consumables support (21) and a consumables picking arm (22). The consumables and equipment preparation chamber (S3) and the specimen preparation chamber (S4) are connected by a consumables feeding module (20). The consumables on the consumables support (21) are placed on the consumables feeding module (20) by the consumables picking arm (22). The specimen preparation chamber (S4) is equipped with a feeding conveyor belt for the corresponding consumable feeding module (20) and the sample tube rack dispensing module (8). The transfer mechanism between the specimen preparation chamber (S4) and the detection and specimen processing chamber (S5) includes a PCR feeding module (18), a waste transfer module (11), and a sampling tube waste transfer module (12). The dispensing end of the pipetting / sealing device (19) is connected to the PCR feeding module (18). The ends of the PCR feeding module (18) and the sampling tube waste transfer module (12) extend into the detection and specimen processing chamber (S5). The sealed samples and sampling tube waste are sent to the detection and specimen processing chamber (S5) for processing through the PCR feeding module (18) and the sampling tube waste transfer module (12).

4. The fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation according to claim 3, characterized in that: The sampling tube loading chamber (S7) and the consumables and equipment preparation chamber (S3) are located in the same unit compartment and are adjacent to each other. The sampling area (S6) is also located in front of the sampling chamber.

5. The fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation according to claim 1, characterized in that: Inspection ports (J4) are provided on the walls of the prefabricated houses of the sampling area (S6), the sampling tube loading room (S7), the specimen preparation room (S4), and the testing and specimen processing room (S5).

6. The fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation according to claim 1, characterized in that: An active sunshade device (J2) is provided outside the sampling window.

7. The fully automated intelligent robot nucleic acid sampling, detection and analysis integrated workstation according to claim 1, characterized in that: The sampling room is also equipped with an equipment room (S1) on the left side. The intelligent server is located in the equipment room (S1). Each unit compartment is equipped with a monitoring device (27). The monitoring data of all monitoring devices (27) are transmitted to the equipment room (S1). The equipment room (S1) is equipped with an entrance door (J1).

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