Broussonetia papyrifera type fermented feed and preparation method thereof

Through the synergistic effect of compound microbial agents and absorption promoters, the problems of poor fermentation effect of paper mulberry and unsatisfactory feed absorption have been solved, thereby improving the nutritional value of paper mulberry fermented feed and the immune level of livestock and poultry.

CN117016674BActive Publication Date: 2025-11-11QINGYUAN POLYTECHNIC
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Patent Information

Application Number
CN202310900523.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-07-21
Publication Date
2025-11-11
Estimated Expiration
2043-07-21

AI Technical Summary

Technical Problem

The existing technology suffers from poor fermentation effect of paper mulberry and unsatisfactory feed absorption.

Method used

A compound microbial agent was used to ferment mulberry trees by mixing Bacillus subtilis, Lactobacillus and Lysimachia christinae, and adding microcapsule-encapsulated microcrystalline cellulose, hawthorn powder and chitosan to promote absorption. Combined with specific fermentation conditions and static treatment, mulberry-type fermented feed was prepared.

Benefits of technology

It improves the fermentation effect of paper mulberry, enhances the digestibility and absorption rate of feed nutrients, improves the taste and meat quality of feed, and enhances the immunity of livestock and poultry.

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Abstract

The application discloses a broussonetia papyrifera type fermented feed and a preparation method thereof, and belongs to the field of feed preparation, and comprises fermented broussonetia papyrifera, corn starch, soybean meal, puffed soybean powder, sodium chloride, calcium hydrogen phosphate, an absorption promoting agent and a premix ingredient, wherein the fermented broussonetia papyrifera is obtained by fermentation treatment of the broussonetia papyrifera by a composite microbial agent. The broussonetia papyrifera is high in crude protein content after the fermentation treatment, and is also rich in nutritional ingredients such as vitamins, carbohydrates and trace elements; the use of the composite microbial agent can help improve the fermentation effect of the broussonetia papyrifera, effectively degrade biological macromolecules such as crude fiber and improve the digestion and absorption rate of nutritional substances; in the preparation process of the feed, after the static treatment, secondary fermentation is equivalent to be carried out, the active peptides and the active polysaccharides in the feed can be improved, and a large number of beneficial active bacteria are retained, so that the feed is not only ensured to be balanced in nutrition, but also is ensured to have a unique flavor, and the feed taste is helped to be improved.
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Description

Technical Field

[0001] This invention relates to the field of feed preparation, and more specifically, to a paper mulberry-based fermented feed and its preparation method. Background Technology

[0002] The tender branches and leaves of the paper mulberry tree are high in protein, making them a promising source of protein feed and alleviating my country's long-standing reliance on imported high-protein feed. Furthermore, paper mulberry can be planted on barren hillsides and slopes, achieving a virtuous cycle of ecological and economic benefits. In addition, existing hybrid paper mulberry varieties are new types developed using modern aerospace technology, including space-borne breeding and hybridization, after nearly 10 years of screening and experimental planting. Compared to traditional paper mulberry, hybrid paper mulberry varieties for forage exhibit rapid growth, exceptional adaptability and resilience, tolerance to poor soil and drought, and resistance to pollution and pests. They are highly productive and possess significant economic value. Paper mulberry leaves are rich in plant crude protein, and the leaves, stems, and bark are rich in natural flavonoids, with a protein content as high as 26.1%, 8% higher than alfalfa meal. Its total energy, digestible energy, metabolizable energy, net energy, and digestible crude protein content are all quite high. It also boasts high levels of crude fat, calcium, amino acids, vitamins, carbohydrates, and trace elements. This is a newly bred woody plant forage resource that can provide higher protein and energy levels, comprehensively improving the immune system of livestock and poultry and reducing mortality. While existing technologies use paper mulberry as a raw material to prepare feed, these generally suffer from poor fermentation and unsatisfactory feed absorption. Summary of the Invention

[0003] Based on this, in order to solve the problems of poor fermentation effect and unsatisfactory feed absorption in the existing technology, the present invention provides a paper mulberry-based fermented feed and its preparation method, the specific technical solution of which is as follows:

[0004] A fermented feed based on paper mulberry, comprising the following components in parts by weight: 30-35 parts fermented paper mulberry, 5-9 parts corn starch, 3-5 parts soybean meal, 5-7 parts extruded soybean flour, 0.2-0.5 parts sodium chloride, 1-4 parts dicalcium phosphate, 1-15 parts absorption promoter, and 3-7 parts premix.

[0005] The fermented paper mulberry is obtained through fermentation with a compound microbial agent, which is a mixture of Bacillus subtilis, Lactobacillus, and Lysimachia christinae, with a concentration of Bacillus subtilis of 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL. 8 The concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1 × 10⁻⁶ CFU / mL. 8 CFU / mL.

[0006] Furthermore, the fermentation process includes the following steps:

[0007] Pre-fermentation treatment of paper mulberry;

[0008] The compound microbial agent was inoculated into the pretreated paper mulberry and fermentation medium was added. Under room temperature conditions, the humidity was maintained at 45% to 48%, and the pH was adjusted to 5 to 6. Fermentation was carried out for 10 to 15 days to obtain fermented paper mulberry.

[0009] Furthermore, the paper mulberry tree includes one or both of hybrid paper mulberry and wild paper mulberry.

[0010] Furthermore, the pre-fermentation treatment involves feeding paper mulberry with a moisture content of 50% to 55% into a pulverizer and pulverizing it to 2cm to 3cm.

[0011] Furthermore, the fermentation medium comprises the following components in parts by weight: 12 to 15 parts modified agar, 3 to 5 parts honey, 9 to 11 parts yeast extract, and 0.1 to 0.5 parts magnesium sulfate heptahydrate.

[0012] Furthermore, the absorption enhancer is composed of microcrystalline cellulose, hawthorn powder, chitosan and sodium alginate in a mass ratio of (1-3):(2-5):(1-2):(5-9).

[0013] In addition, this application also provides a method for preparing paper mulberry-based fermented feed, the preparation method comprising the following steps:

[0014] Fermented mulberry, corn starch, soybean meal, puffed soybean flour, sodium chloride, dicalcium phosphate, and premixed materials are fed into a mixer and stirred for 30 to 40 minutes to obtain a mixture.

[0015] An absorption enhancer was prepared and then added to the mixture. After stirring for 15 to 20 minutes, the mixture was allowed to stand, decomposed, and granulated to obtain mulberry-type fermented feed.

[0016] Furthermore, the method for preparing the absorption enhancer is as follows: microcrystalline cellulose, hawthorn powder and chitosan are mixed, ball-milled until the average particle size is ≤50μm, sodium alginate is added, and the mixture is spray-dried to obtain a microcapsule-encapsulated absorption enhancer.

[0017] Furthermore, the settling time is 3 to 5 days.

[0018] The above-mentioned scheme, through the fermentation treatment of paper mulberry, results in a high crude protein content, as well as rich amino acids, vitamins, carbohydrates, and trace elements. The use of compound microbial agents helps improve the fermentation effect of paper mulberry and effectively degrades crude fiber and other biomolecules, increasing the digestibility and absorption rate of nutrients. In the feed preparation process, the static treatment is equivalent to a secondary fermentation, which increases the bioactive peptides and polysaccharides in the feed and retains a large number of beneficial live bacteria. This not only ensures the balanced nutrition of the feed but also preserves its unique flavor, helping to improve its palatability. Furthermore, this application adds an absorption promoter, which is prepared in microcapsule form. Its slow-release effect, combined with the synergistic effect of the compound microbial agents, helps improve feed absorption and growth promotion, while also improving meat quality and enhancing immunity. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to its embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the scope of protection of the invention.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0021] A fermented feed of paper mulberry type according to one embodiment of the present invention comprises the following components in parts by weight: 30-35 parts fermented paper mulberry, 5-9 parts corn starch, 3-5 parts soybean meal, 5-7 parts extruded soybean flour, 0.2-0.5 parts sodium chloride, 1-4 parts dicalcium phosphate, 1-15 parts absorption promoter, and 3-7 parts premix.

[0022] The fermented paper mulberry is obtained through fermentation with a compound microbial agent, which is a mixture of Bacillus subtilis, Lactobacillus, and Lysimachia christinae, with a concentration of Bacillus subtilis of 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL. 8 The concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1 × 10⁻⁶ CFU / mL. 8 CFU / mL.

[0023] In one embodiment, the fermentation process includes the following steps:

[0024] Pre-fermentation treatment of paper mulberry;

[0025] The compound microbial agent was inoculated into the pretreated paper mulberry and fermentation medium was added. Under room temperature conditions, the humidity was maintained at 45% to 48%, and the pH was adjusted to 5 to 6. Fermentation was carried out for 10 to 15 days to obtain fermented paper mulberry.

[0026] In one embodiment, the paper mulberry tree includes one or both of hybrid paper mulberry and wild paper mulberry.

[0027] In one embodiment, the pre-fermentation treatment is as follows: paper mulberry with a moisture content of 50% to 55% is fed into a pulverizer and pulverized to 2cm to 3cm.

[0028] In one embodiment, the fermentation medium comprises the following components in parts by weight: 12 to 15 parts modified agar, 3 to 5 parts honey, 9 to 11 parts yeast extract, and 0.1 to 0.5 parts magnesium sulfate heptahydrate.

[0029] In one embodiment, the absorption enhancer is composed of microcrystalline cellulose, hawthorn powder, chitosan and sodium alginate in a mass ratio of (1-3):(2-5):(1-2):(5-9).

[0030] In addition, this application also provides a method for preparing paper mulberry-based fermented feed, the preparation method comprising the following steps:

[0031] Fermented mulberry, corn starch, soybean meal, puffed soybean flour, sodium chloride, dicalcium phosphate, and premixed materials are fed into a mixer and stirred for 30 to 40 minutes to obtain a mixture.

[0032] An absorption enhancer was prepared and then added to the mixture. After stirring for 15 to 20 minutes, the mixture was allowed to stand, decomposed, and granulated to obtain mulberry-type fermented feed.

[0033] In one embodiment, the absorption enhancer is prepared by mixing microcrystalline cellulose, hawthorn powder and chitosan, ball milling to an average particle size ≤50μm, then adding sodium alginate, and spray drying to obtain a microcapsule-encapsulated absorption enhancer.

[0034] In one embodiment, the settling time is 3 to 5 days.

[0035] The above-mentioned scheme, through the fermentation treatment of paper mulberry, results in a high crude protein content, as well as rich amino acids, vitamins, carbohydrates, and trace elements. The use of compound microbial agents helps improve the fermentation effect of paper mulberry and effectively degrades crude fiber and other biomolecules, increasing the digestibility and absorption rate of nutrients. In the feed preparation process, the static treatment is equivalent to a secondary fermentation, which increases the bioactive peptides and polysaccharides in the feed and retains a large number of beneficial live bacteria. This not only ensures the balanced nutrition of the feed but also preserves its unique flavor, helping to improve its palatability. Furthermore, this application adds an absorption promoter, which is prepared in microcapsule form. Its slow-release effect, combined with the synergistic effect of the compound microbial agents, helps improve feed absorption and growth promotion, while also improving meat quality and enhancing immunity.

[0036] The implementation schemes of the present invention will now be described in detail with reference to specific embodiments.

[0037] Example 1:

[0038] A method for preparing a paper mulberry-based fermented feed includes the following steps:

[0039] The compound bacterial agent was prepared by mixing according to the following concentrations; the concentration of Bacillus subtilis was 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL. 8 The concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1 × 10⁻⁶ CFU / mL. 8 CFU / mL;

[0040] Hybrid paper mulberry with a moisture content of 55% was fed into a pulverizer and pulverized to 2-3 cm.

[0041] The compound microbial agent was inoculated into the pretreated paper mulberry and a fermentation medium was added. The fermentation medium consisted of the following components by weight: 12 parts modified agar, 5 parts honey, 9 parts yeast extract, and 0.1 parts magnesium sulfate heptahydrate. Fermentation was carried out at room temperature, with humidity maintained at 45% and pH adjusted to 6 for 15 days to obtain fermented paper mulberry.

[0042] 35 parts of fermented paper mulberry, 5 parts of corn starch, 3 parts of soybean meal, 5 parts of puffed soybean flour, 0.2 parts of sodium chloride, 1 part of dicalcium phosphate, and 3 parts of premix were fed into a mixer and stirred for 40 minutes to obtain a mixture.

[0043] Microcrystalline cellulose, hawthorn powder and chitosan were mixed and ball-milled until the average particle size was ≤50μm. Sodium alginate was then added and spray-dried to obtain 12 parts of microcapsule-encapsulated absorption promoter. The absorption promoter was then added to the mixture and stirred for 15 minutes. After standing for 5 days, the mixture was decomposed and granulated to obtain mulberry-type fermented feed.

[0044] Example 2:

[0045] A method for preparing a paper mulberry-based fermented feed includes the following steps:

[0046] The compound bacterial agent was prepared by mixing according to the following concentrations; the concentration of Bacillus subtilis was 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL. 8 The concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1 × 10⁻⁶ CFU / mL. 8 CFU / mL;

[0047] Hybrid paper mulberry with a moisture content of 52% was fed into a pulverizer and pulverized to 2-3 cm.

[0048] The compound microbial agent was inoculated into the pretreated paper mulberry and a fermentation medium was added. The fermentation medium consisted of the following components by weight: 14 parts modified agar, 4 parts honey, 10 parts yeast extract, and 0.3 parts magnesium sulfate heptahydrate. Fermentation was carried out at room temperature, with humidity maintained at 45% and pH adjusted to 5-6 for 12 days to obtain fermented paper mulberry.

[0049] 32 parts of fermented paper mulberry, 7 parts of corn starch, 4 parts of soybean meal, 5 parts of puffed soybean flour, 0.4 parts of sodium chloride, 3 parts of dicalcium phosphate, and 6 parts of premix were fed into a mixer and stirred for 35 minutes to obtain a mixture.

[0050] Microcrystalline cellulose, hawthorn powder and chitosan were mixed and ball-milled until the average particle size was ≤50μm. Sodium alginate was then added and spray-dried to obtain 12 parts of microcapsule-encapsulated absorption promoter. The absorption promoter was then added to the mixture and stirred for 18 minutes. After standing for 4 days, the mixture was decomposed and granulated to obtain mulberry-type fermented feed.

[0051] Example 3:

[0052] A method for preparing a paper mulberry-based fermented feed includes the following steps:

[0053] The compound bacterial agent was prepared by mixing according to the following concentrations; the concentration of Bacillus subtilis was 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL. 8 The concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1 × 10⁻⁶ CFU / mL. 8 CFU / mL;

[0054] Hybrid paper mulberry with a moisture content of 54% was fed into a pulverizer and pulverized to 2-3 cm.

[0055] The compound microbial agent was inoculated into the pretreated paper mulberry and a fermentation medium was added. The fermentation medium consisted of the following components by weight: 13 parts modified agar, 5 parts honey, 10 parts yeast extract, and 0.4 parts magnesium sulfate heptahydrate. Fermentation was carried out at room temperature, with humidity maintained at 47% and pH adjusted to 5-6 for 14 days to obtain fermented paper mulberry.

[0056] 33 parts of fermented paper mulberry, 7 parts of corn starch, 5 parts of soybean meal, 5 parts of puffed soybean flour, 0.4 parts of sodium chloride, 3 parts of dicalcium phosphate, and 5 parts of premix were fed into a mixer and stirred for 37 minutes to obtain a mixture.

[0057] Microcrystalline cellulose, hawthorn powder and chitosan were mixed and ball-milled until the average particle size was ≤50μm. Sodium alginate was then added and spray-dried to obtain 12 parts of microcapsule-encapsulated absorption promoter. The absorption promoter was then added to the mixture and stirred for 18 minutes. After standing for 4 days, the mixture was decomposed and granulated to obtain mulberry-type fermented feed.

[0058] The composition and ratio of the absorption enhancers in Examples 1-3 are shown in Table 1 below.

[0059] Comparative Examples 1-4:

[0060] The difference between Comparative Examples 1-4 and Example 3 is that the components and proportions of the absorption promoter are different, as shown in Table 1. Everything else is the same as Example 3.

[0061] Comparative Example 5:

[0062] The difference between Comparative Example 5 and Example 3 is that no absorption enhancer was added in Comparative Example 5, while the rest is the same as in Example 3.

[0063] Comparative Examples 6–9:

[0064] The difference between Comparative Examples 6-9 and Example 3 is that the compound microbial agent added in Comparative Examples 6-9 is different from that in Example 3, as shown in Table 2.

[0065] Comparative Example 10:

[0066] The difference between Comparative Example 10 and Example 3 is that the absorption enhancer in Comparative Example 10 was not microencapsulated. The specific preparation method is as follows:

[0067] A method for preparing a paper mulberry-based fermented feed includes the following steps:

[0068] The compound bacterial agent was prepared by mixing according to the following concentrations; the concentration of Bacillus subtilis was 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL, and the concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1×10 CFU / mL.8 CFU / mL;

[0069] Hybrid paper mulberry with a moisture content of 54% was fed into a pulverizer and pulverized to 2-3 cm.

[0070] The compound microbial agent was inoculated into the pretreated paper mulberry and a fermentation medium was added. The fermentation medium consisted of the following components by weight: 13 parts modified agar, 5 parts honey, 10 parts yeast extract, and 0.4 parts magnesium sulfate heptahydrate. Fermentation was carried out at room temperature, with humidity maintained at 47% and pH adjusted to 5-6 for 14 days to obtain fermented paper mulberry.

[0071] 33 parts of fermented paper mulberry, 7 parts of corn starch, 5 parts of soybean meal, 5 parts of puffed soybean flour, 0.4 parts of sodium chloride, 3 parts of dicalcium phosphate, and 5 parts of premix were fed into a mixer and stirred for 37 minutes to obtain a mixture.

[0072] Microcrystalline cellulose, hawthorn powder, chitosan and sodium alginate were added to the mixture, stirred for 18 minutes, and then allowed to stand for 4 days to obtain mulberry-type fermented feed.

[0073] Table 1:

[0074]

[0075]

[0076] Table 2:

[0077]

[0078] The fermented paper mulberry-type feeds obtained in Examples 1-3 and the fermented paper mulberry-type feeds prepared in Comparative Examples 1-10 were tested. The crude protein and crude fiber contents were determined according to the methods in GB / T6432-1994 and GB / T6434-2006, respectively. The results are shown in Table 3 below.

[0079] Table 3:

[0080]

[0081]

[0082] It should be noted that: crude protein increase rate = (crude protein content after fermentation - crude protein content before fermentation) / crude protein content before fermentation;

[0083] Crude fiber degradation rate = (cellulose content before fermentation - cellulose content after fermentation) / cellulose content before fermentation;

[0084] Active polypeptide proliferation rate = (active polypeptide content before fermentation - active polypeptide content after fermentation) / active polypeptide content before fermentation;

[0085] Active polysaccharide proliferation rate = (active polysaccharide content before fermentation - active polysaccharide content after fermentation) / active polysaccharide content before fermentation.

[0086] As can be seen from the data analysis in Table 3, after the compound microbial agent fermentation treatment, the fermented feed of this application has a high crude protein proliferation rate, active polypeptide proliferation rate and active polysaccharide proliferation rate, and has an excellent crude fiber degradation rate. The difference between Comparative Examples 1-5 and Example 3 lies in the different components and proportions of the absorption promoter, which have a relatively small impact on the overall content of crude protein, active peptides, and active polysaccharides in the fermented feed. The difference between Comparative Examples 6-9 and Example 3 lies in the different compound microbial agents added. However, it can be seen that the crude protein proliferation rate, active peptide proliferation rate, and active polysaccharide proliferation rate are significantly reduced in Comparative Examples 6-9, and the crude fiber degradation rate is also significantly worse than that in Example 3. This indicates that the components of the compound microbial agent in this application affect the fermentation effect, leading to changes in the nutritional composition of the fermented feed. The difference between Comparative Example 10 and Example 3 lies in the fact that the absorption promoter was not microencapsulated. The crude protein proliferation rate, active peptide proliferation rate, and active polysaccharide proliferation rate are not significantly different from those in Example 3, and the crude fiber degradation rate is also not significantly different. This indicates that the components and proportions of the compound microbial agent in this application have a significant impact on the nutritional composition of the fermented feed. Furthermore, the different strains of the compound microbial agent work synergistically to achieve a significant fermentation effect and impart higher nutritional value to the fermented feed.

[0087] Application Experiment:

[0088] Grass carp were used as the experimental subject. Thirteen experimental groups were set up, and they were fed fermented feed of proportions 1 to 3 and comparative proportions 1 to 10, respectively. A control group was set up and fed commercial feed.

[0089] After stocking grass carp fry, the daily feed amount (using fermented feed ratios 1-3 and 1-10 respectively, with the control group fed commercial feed) was 3.0% of the fish population weight. The feed amount was adjusted every 7 days, increasing by 5% each time, and minor adjustments were made based on water temperature, weather, and feeding behavior. Feeding was conducted twice daily, at 08:30 and 16:30. Ammonia nitrogen and pH were monitored twice weekly, with regular water replenishment to ensure ammonia nitrogen remained below 0.5 mg / L and pH was maintained between 7.3 and 7.9.

[0090] After 45 days of feeding, each group was tested separately. The meat quality indicators were based on the corresponding national testing standards (GB / T9695.7-2008, GB / T9695.18-2008, GB / T9695.15-2008 and GB / T9695.11-2008). The results are shown in Table 4 below (average).

[0091] Table 4:

[0092]

[0093]

[0094] Analysis of the data in Table 4 shows that the fermented feed of this application, through the synergistic effect of the absorption promoter, can promote the growth of grass carp and result in higher meat quality. Comparative Examples 1-5 demonstrate that changes in the composition and ratio of the absorption promoter can affect the growth of grass carp, indicating that the optimized composition of this application can also achieve a synergistic effect (1+1>2). Furthermore, the optimized compounding of microbial strains not only provides the fermented feed with rich nutrients, but also, under the action of the absorption promoter, these abundant nutrients are easily absorbed by the body. In addition, the absorption promoter of this application is further encapsulated in microcapsules, which helps it exert a slow-release effect, achieving significant feeding results.

[0095] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0096] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A fermented feed based on the paper mulberry tree, characterized in that, It comprises the following components by weight: 30-35 parts fermented paper mulberry, 5-9 parts corn starch, 3-5 parts soybean meal, 5-7 parts puffed soybean flour, 0.2-0.5 parts sodium chloride, 1-4 parts dicalcium phosphate, 1-15 parts absorption promoter, and 3-7 parts premix. The fermented paper mulberry is obtained through fermentation with a compound microbial agent, which is a mixture of Bacillus subtilis, Lactobacillus, and Lysimachia christinae, with a concentration of Bacillus subtilis of 2 × 10⁻⁶. 9 The concentration of Lactobacillus in the compound bacterial agent is 1×10 CFU / mL. 8 The concentration of *Saccharomyces cerevisiae* in the compound bacterial agent is 1 × 10⁻⁶ CFU / mL. 8 CFU / mL; The absorption enhancer is composed of microcrystalline cellulose, hawthorn powder, chitosan and sodium alginate in a mass ratio of (1-3):(2-5):(1-2):(5-9); The method for preparing the absorption enhancer is as follows: microcrystalline cellulose, hawthorn powder and chitosan are mixed, ball-milled until the average particle size is ≤50μm, sodium alginate is added, and the mixture is spray-dried to obtain a microcapsule-encapsulated absorption enhancer.

2. The paper mulberry-type fermented feed according to claim 1, characterized in that, The fermentation process includes the following steps: Pre-fermentation treatment of paper mulberry; The compound microbial agent was inoculated into the pretreated paper mulberry and fermentation medium was added. Under room temperature conditions, the humidity was maintained at 45% to 48%, and the pH was adjusted to 5 to 6. Fermentation was carried out for 10 to 15 days to obtain fermented paper mulberry.

3. The paper mulberry-type fermented feed according to claim 1, characterized in that, The paper mulberry tree includes one or both of hybrid paper mulberry and wild paper mulberry.

4. The paper mulberry-type fermented feed according to claim 1, characterized in that, The pre-fermentation treatment is as follows: paper mulberry with a moisture content of 50% to 55% is fed into a pulverizer and pulverized to 2cm to 3cm.

5. The paper mulberry-type fermented feed according to claim 1, characterized in that, The fermentation medium comprises the following components by weight: 12-15 parts modified agar, 3-5 parts honey, 9-11 parts yeast extract, and 0.1-0.5 parts magnesium sulfate heptahydrate.

6. A method for preparing a paper mulberry-based fermented feed, characterized in that, The preparation method is used to prepare the paper mulberry-type fermented feed as described in any one of claims 1 to 5, and the preparation method includes the following steps: Fermented mulberry, corn starch, soybean meal, puffed soybean flour, sodium chloride, dicalcium phosphate, and premixed materials are fed into a mixer and stirred for 30 to 40 minutes to obtain a mixture. An absorption enhancer was prepared and then added to the mixture. After stirring for 15 to 20 minutes, the mixture was allowed to stand, decomposed, and granulated to obtain mulberry-type fermented feed.

7. The preparation method according to claim 6, characterized in that, The settling time is 3 to 5 days.

Citation Information

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