Embryo activity preservation device for bovine embryo transfer

By introducing a placement platform and clamping components into the bovine embryo viability preservation device, the problem of rope entanglement in the frozen semen bag was solved, enabling safe and convenient removal of bovine embryos and avoiding frostbite and contamination.

CN117136944BActive Publication Date: 2025-11-04XINJIANG HAOZI ANIMAL HUSBANDRY CO LTD
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Patent Information

Application Number
CN202311130160.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-09-04
Publication Date
2025-11-04
Estimated Expiration
2043-09-04

AI Technical Summary

Technical Problem

In existing bovine embryo viability preservation devices, the ropes of the frozen semen bags are prone to tangling, making removal inconvenient, and manual operation can easily cause frostbite and contamination of the test tubes.

Method used

The design incorporates a placement platform, adjustment components, and clamping components. The clamping components hold the bucket in place for easy loading and unloading, replacing the storage method of frozen semen bags and ropes, thus avoiding rope tangling and manual operation.

Benefits of technology

This effectively avoids the tangling of multiple frozen sperm bags, prevents frostbite on hands and contamination of test tubes, and improves the convenience and safety of the operation.

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Abstract

The application discloses a kind of embryo active storage devices for bovine embryo transplantation, the liquid nitrogen tank includes shell, inner container and neck pipe, the inner container is located inside shell, the gap between shell and inside is interlayer, the neck pipe is installed at the top of inner container, the top of neck pipe penetrates and extends to the top of shell and is provided with a cap; The placing table is provided with several and circularly arrayed in the inside of inner container, and the adjusting assembly for driving the placing table to move is installed between the side of placing table and the inner wall of inner container. The adjusting assembly moves the placing table to the position directly below the neck pipe, and the bucket is clamped and placed by clamping assembly. The bucket on the placing table can also be taken out by clamping assembly, instead of using frozen semen cloth bag storage and fixed by rope, so that the situation that multiple ropes are wound together and are inconvenient to take out does not occur, effectively avoiding the hand frostbite of staff and the situation of test tube pollution.
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Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bovine embryo transfer, in particular to an embryo active preservation device for bovine embryo transfer. BACKGROUND

[0002] Embryo transfer, also known as zygote transfer, is a technology that early embryos of female animals or embryos obtained by in vitro fertilization and other means are transplanted into female animals to develop into new individuals. Among them, embryo transfer is widely used in animal breeding field, mainly to improve the breeding speed of animals and shorten the generation interval. Bovine embryo transfer is an effective means to rapidly increase the number of high-yield and high-quality dairy cows and promote the development of the dairy industry, and is a high-tech currently applied in dairy production.

[0003] At present, the common active preservation device on the market basically uses a liquid nitrogen tank to freeze bovine embryos, and when needed, the frozen embryos are taken out for thawing and transplantation. In actual use, the test tube containing bovine embryos is placed in a frozen semen cloth bag, the frozen semen cloth bag is placed in the liquid nitrogen tank, and the rope on the frozen semen cloth bag is fixed outside the liquid nitrogen tank.

[0004] When needed, the frozen semen cloth bag is taken out by pulling the rope. In this way, since a large number of frozen semen cloth bags are stored, the ropes on the frozen semen cloth bags may be entangled together for a long time. At this time, pulling one of the ropes may pull out multiple frozen semen cloth bags, and the multiple frozen semen cloth bags converge at the neck pipe to block the neck pipe and cannot be taken out. In addition, the rope needs to be pulled manually. Since part of the rope is placed in the liquid nitrogen tank, when the rope is pulled manually, the hands of the workers are easily frozen and the embryos in the test tube are easily contaminated. SUMMARY

[0005] In order to overcome the shortcomings of the prior art, the present application provides an embryo active preservation device for bovine embryo transfer. The device is internally provided with a placement table, a bucket, an adjusting assembly and a clamping assembly. The adjusting assembly moves the placement table directly below the neck pipe, and the clamping assembly clamps the bucket for placement. The bucket on the placement table can also be taken out through the clamping assembly, replacing the storage by frozen semen cloth bags and the fixed way by ropes. This avoids the situation that multiple ropes are entangled together and are difficult to take out, and effectively avoids the situation that the hands of the workers are frozen and the test tubes are contaminated.

[0006] To solve the above technical problems, the present application provides the following technical scheme: an embryo active preservation device for bovine embryo transfer, comprising:

[0007] A liquid nitrogen tank, the liquid nitrogen tank comprises an outer shell, an inner container and a neck pipe, the inner container is located in the inside of the outer shell, the gap between the outer shell and the inner side is a sandwich, the neck pipe is installed at the top of the inner container, the top end of the neck pipe penetrates and extends to the top of the outer shell and is provided with a plug;

[0008] The placing table is provided with a plurality of placing tables which are arranged in a circumferential array inside the inner container, and an adjusting assembly for driving the placing table to move is arranged between one side of the placing table and the inner wall of the inner container.

[0009] The bucket is placed on the placing table, and the bottom of the cover is provided with a clamping assembly for clamping the bucket.

[0010] As a preferred technical scheme of the present application, a plurality of through holes are formed in the surface of the placing table, a plurality of magnetic strips are fixedly arranged on the upper surface of the placing table, a fence is fixedly arranged between the magnetic strips, the bucket is made of a stainless steel mesh material, and the bucket can be clamped in the fence and is attracted by the magnetic strips.

[0011] As a preferred technical scheme of the present application, each adjusting assembly comprises a connecting frame fixed to the inner wall of the inner container, a support frame fixed to the lower side of one end of the connecting frame, a rotating shaft A rotatably connected to the inner wall of the inner container, a worm gear rotatably connected to the inner side of the connecting frame, a worm fixed to one end of the rotating shaft, and a toothed plate slidably connected to the support frame.

[0012] As a preferred technical scheme of the present application, the worm and the worm gear are in mesh with each other, the toothed plate and the gear A are in mesh with each other, the placing table is fixed to one end of the toothed plate, the support frame is provided with a guide rod A slidably connected thereto inside the support frame, and both ends of the guide rod A are fixed to the bottom of the toothed plate.

[0013] As a preferred technical scheme of the present application, one end of the rotating shaft extends to the outside of the shell, a sealing bearing is arranged between the rotating shaft and the shell and the inner container, a knob is fixedly connected to one end of the rotating shaft, and a label is arranged on the end surface of the knob.

[0014] As a preferred technical scheme of the present application, a circular hole and a groove are formed in the top and the bottom of the cover, respectively, the clamping assembly comprises a rotating shaft B rotatably arranged in the groove, a gear B and a rotating disc fixedly connected to the outer wall of the rotating shaft B, a guide rod B fixedly arranged in the groove, a clamping rod slidably arranged at both ends of the guide rod B, a connecting rod hingedly connected between the clamping rod and the rotating disc, a pressing rod inserted into the circular hole, a rack fixed to the bottom end of the pressing rod and in mesh with the gear B, and a spring sleeved outside the pressing rod.

[0015] As a preferred technical scheme of the present application, a piston is fixedly sleeved on the outer wall of the pressing rod, the piston slides along the inner wall of the circular hole and seals the circular hole, and the spring is fixed between the inner bottom wall of the circular hole and the bottom of the piston.

[0016] As a preferred technical scheme of the present application, an annular groove is formed in the upper wall of the bucket, and the bottom end of the clamping rod can be inserted into the annular groove.

[0017] As a preferred technical scheme of the present application, the top end of the outer wall of the neck pipe is provided with a thread line, the top end of the neck pipe is threadedly connected with a cover through the thread line, and the inner top wall of the cover does not contact the top end of the pressure rod.

[0018] As a preferred technical scheme of the present application, the shell, the inner container and the neck pipe are all made of hard alloy material, the inside of the inner container stores liquid nitrogen, and the interlayer is processed by vacuumizing and filled with heat insulation material and adsorbent.

[0019] Compared with the prior art, the present application has the following beneficial effects:

[0020] 1. The method of storing and fixing by using frozen semen cloth bag and rope is replaced: the placing table is moved to the position directly below the neck pipe through the adjusting assembly, and the bucket is taken out from the liquid nitrogen tank by the clamping assembly, so that the method of storing and fixing by using frozen semen cloth bag and rope in the existing bovine embryo active preservation device is replaced, and the situation that multiple ropes are wound together and the taking out is inconvenient does not occur.

[0021] 2. The hands of the staff are effectively prevented from being frozen: the clamping assembly is used to insert the clamping rod in the clamping assembly into the annular groove above the outer wall of the bucket by pressing the pressure rod, so that the bucket is clamped, and the taking and placing of the bucket are performed, so that the staff does not need to manually take out the test tube containing bovine embryos from the liquid nitrogen tank, and the hands are prevented from being frozen.

[0022] 3. The test tube is effectively prevented from being contaminated: the taking and placing of the bucket are performed by the clamping assembly, so that the hands of the staff do not contact the bucket, and the hands of the staff are effectively prevented from contacting the bucket to contaminate the test tube in the bucket. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a structure perspective state schematic view of the present application;

[0024] Figure 2 It is a sectional structure perspective state schematic view of the present application

[0025] Figure 3 It is a sectional structure perspective state schematic view of the present application Figure 2 It is an enlarged state schematic view of A in the present application;

[0026] Figure 4 It is a front perspective state schematic view of the clamping assembly of the present application;

[0027] Figure 5 It is a rear perspective state schematic view of the clamping assembly of the present application;

[0028] Figure 6 It is a perspective state schematic view of the adjusting assembly of the present application;

[0029] Figure 7This is a three-dimensional view of the adjustment component of the present invention from a low angle;

[0030] Figure 8 This is a three-dimensional schematic diagram of the placement platform and toothed plate of the present invention;

[0031] Figure 9 This is a three-dimensional schematic diagram of the bucket of the present invention.

[0032] The components are as follows: 1. Liquid nitrogen tank; 11. Outer shell; 12. Inner liner; 13. Neck tube; 14. Interlayer; 15. Plug; 2. Placement platform; 21. Through hole; 22. Magnetic strip; 23. Fence; 3. Adjustment assembly; 31. Connecting frame; 32. Support frame; 33. Rotating rod; 34. Rotating shaft A; 35. Worm gear; 36. Gear A; 37. Worm; 38. Tooth plate; 39. Guide rod A; 310. Knob; 4. Lifting bucket; 41. Annular groove; 5. Clamping assembly; 51. Rotating shaft B; 52. Gear B; 53. Turntable; 54. Guide rod B; 55. Clamping rod; 56. Connecting rod; 57. Pressure rod; 58. Rack; 59. Spring; 510. Piston; 6. Round hole; 7. Groove; 8. Cover. Detailed Implementation

[0033] To make the technical means, creative features, and achieved objectives and effects of this invention easier to understand, the invention is further described below with reference to specific embodiments. However, the following embodiments are merely preferred embodiments of this invention and not all of them. Other embodiments obtained by those skilled in the art based on the embodiments described herein without creative effort are all within the protection scope of this invention. Unless otherwise specified, the experimental methods in the following embodiments are conventional methods, and the materials and reagents used in the following embodiments are commercially available unless otherwise specified.

[0034] Example:

[0035] like Figure 1 - Figure 9 As shown in the figure, this embodiment proposes an embryo viability preservation device for bovine embryo transfer, including a liquid nitrogen tank 1, a placement platform 2, and a lifting bucket 4.

[0036] The liquid nitrogen tank 1 includes an outer shell 11, an inner liner 12, and a neck tube 13. The inner liner 12 is located inside the outer shell 11, and the gap between the outer shell 11 and the inner side is a sandwich 14. The neck tube 13 is installed on the top of the inner liner 12, and the top end of the neck tube 13 penetrates through and extends to the top of the outer shell 11 and is provided with a cap 15. Several placement platforms 2 are arranged in a circumferential array inside the inner liner 12. An adjustment component 3 for driving the placement platform 2 to move is installed between one side of the placement platform 2 and the inner wall of the inner liner 12. A bucket 4 is placed on the placement platform 2, and a clamping component 5 for clamping the bucket 4 is installed at the bottom of the cap 15.

[0037] likeFigure 2 and Figure 8 As shown, the surface of the placement platform 2 is provided with several through holes 21, and several magnetic strips 22 are fixedly installed on the upper surface of the placement platform 2. A fence 23 is fixedly installed between the magnetic strips 22. The bucket 4 is a stainless steel metal mesh component. The bucket 4 can be locked in the fence 23 and attracted by the magnetic strips 22.

[0038] When in use, the bucket 4 is inserted into the fence 23, which initially fixes the bucket 4. At the same time, the magnetic strip 22 attracts the bucket 4, which is made of stainless steel metal mesh, and fixes the bucket 4 between the placement platform 2 and the fence 23. It is not easy to fall off, which is conducive to the placement of the bucket 4.

[0039] like Figure 2 , Figure 6 and Figure 7 As shown, each adjustment component 3 includes a connecting frame 31 fixed to the inner wall of the inner liner 12, a support frame 32 fixed below one end of the connecting frame 31, a rotating rod 33 rotatably connected to the inner wall of the inner liner 12, a rotating shaft A34 rotatably connected to the inner side of the connecting frame 31, a worm gear 35 and a gear A36 fixedly sleeved on the outer wall of the rotating shaft A34, a worm 37 fixed to one end of the rotating rod 33, and a toothed plate 38 that slides with the support frame 32; the worm 37 and the worm gear 35 mesh with each other, the toothed plate 38 and the gear A36 mesh with each other, the placement platform 2 is fixed to one end of the toothed plate 38, and a guide rod A39 that slides with the support frame 32 is inserted inside the support frame 32, with both ends of the guide rod A39 fixed to the bottom of the toothed plate 38;

[0040] In use, the worm 37 is driven to rotate by the drive rod 33. The worm 37 meshes with the worm wheel 35, which drives the rotating shaft A34 to rotate. The gear A36 rotates with the rotating shaft A34. Since the gear A36 meshes with the toothed plate 38, the toothed plate 38 can be driven to push the placement platform 2 to move directly below the neck tube 13, so as to facilitate the picking and placing of the bucket 4.

[0041] like Figure 1 and Figure 7 As shown, one end of the rotating rod 33 passes through and extends to the outside of the outer shell 11. A sealed bearing is installed between the rotating rod 33, the outer shell 11, and the inner liner 12. A knob 310 is fixedly connected to one end of the rotating rod 33. A label is provided on the end face of the knob 310.

[0042] During use, the rotating rod 33 is sealed with the outer shell 11 and the inner liner 12 by a sealed bearing to prevent liquid nitrogen leakage. The knob 310 can easily control the rotation of the rotating rod 33, and the markings on the end face of the knob 310 can provide prompts for precise picking and putting as needed.

[0043] like Figure 2 , Figure 3 ,Figure 4 , Figure 5 and Figure 9 As shown, the top and bottom of the cap 15 are respectively provided with a round hole 6 and a groove 7. The clamping assembly 5 includes a rotating shaft B51 that is longitudinally rotatably disposed inside the groove 7, a gear B52 and a turntable 53 that are fixedly sleeved on the outer wall of the rotating shaft B51, a guide rod B54 that is laterally fixed in the groove 7, a locking rod 55 that is slidably disposed at both ends of the guide rod B54, a connecting rod 56 that is hinged between the locking rod 55 and the turntable 53, a pressure rod 57 that is inserted into the round hole 6, a rack 58 that is fixed at the bottom end of the pressure rod 57 and meshes with the gear B52, and a spring 59 that is sleeved on the outside of the pressure rod 57. An annular groove 41 is provided above the outer wall of the bucket 4, and the bottom end of the locking rod 55 can be inserted into the annular groove 41.

[0044] In use, by pressing down on the lever 57, the rack 58 moves downward with the lever 57 and meshes with the gear B52, which in turn causes the gear B52 to drive the rotating shaft B51 to rotate. The turntable 53 rotates with the rotating shaft B51. When the turntable 53 rotates, it can pull the connecting rod 56. The connecting rod 56 pulls the locking rod 55 to move along the guide rod B54. The locking rod 55 can be inserted into the annular groove 41 above the outer wall of the bucket 4 to clamp the bucket 4, thereby enabling the bucket 4 to be picked up and put down.

[0045] like Figure 3 , Figure 4 and Figure 5 As shown, a piston 510 is fixedly sleeved on the outer wall of the pressure rod 57. The piston 510 slides along the inner wall of the circular hole 6 and seals the circular hole 6. The spring 59 is fixed between the inner bottom wall of the circular hole 6 and the bottom of the piston 510.

[0046] When in use, the movement of the pressure rod 57 will drive the piston 510 to move. The piston 510 can squeeze the spring 59. After the pressure rod 57 is released, the spring 59 can push the piston 510 to reset the pressure rod 57. The piston 510 plays a sealing role while moving.

[0047] As Figure 1 and Figure 2 As shown, the top of the outer wall of the neck tube 13 is provided with a threaded line, and the top of the neck tube 13 is threadedly connected to the cap 8 through the threaded line. The inner top wall of the cap 8 does not contact the top of the pressure rod 57. The outer shell 11, the inner liner 12 and the neck tube 13 are all made of hard alloy material. The inner liner 12 stores liquid nitrogen inside. The interlayer 14 is vacuumed and filled with heat insulation material and adsorbent.

[0048] Working principle and usage process of this invention:

[0049] Firstly, the test tube containing the bovine embryo is placed in the bucket 4, and by rotating one of the rotating rods 33, the worm gear 37 is rotated, the worm gear 37 is engaged with the worm wheel 35 to make the worm wheel 35 drive the rotating shaft A 34 to rotate, the gear A 36 rotates with the rotating shaft A 34, and since the gear A 36 and the toothed plate 38 are engaged, the toothed plate 38 can be driven to move the corresponding placement table 2 to the directly below the neck pipe 13;

[0050] Subsequently, by pressing the pressing rod 57 on the cover plug 15 downward, the rack 58 moves downward with the pressing rod 57 and is engaged with the gear B 52, thereby making the gear B 52 drive the rotating shaft B 51 to rotate, the rotating disc 53 rotates with the rotating shaft B 51, and the rotating disc 53 can pull the connecting rod 56 when rotating, the connecting rod 56 pulls the clamping rod 55 to move along the guide rod B 54, and the clamping rod 55 can be inserted into the annular groove 41 above the outer wall of the bucket 4 to clamp the bucket 4;

[0051] Then, the cover plug 15 with the clamped bucket 4 below is placed in the neck pipe 13, and the bucket 4 is put into the inner container 12 through the neck pipe 13, at this time, there is a placement table 2 directly below the neck pipe 13, the bucket 4 can be clamped into the fence 23 on the placement table 2, the fence 23 preliminarily fixes the bucket 4, and at the same time, the magnetic stripe 22 adsorbs the bucket 4 made of stainless steel mesh material to fix the bucket 4 between the placement table 2 and the fence 23;

[0052] Finally, the pressing rod 57 is loosened, the pressing rod 57 is pushed back to the original position under the action of the spring 59, the clamping rod 55 is moved out of the annular groove 41, and the cover plug 15 is taken out, the rotating rod 33 is reversed to reset the placement table 2, and the bucket 4 is moved to the liquid nitrogen and is not located directly below the neck pipe 13;

[0053] The above steps are repeatedly operated, and according to the process of “adjusting the assembly 3 to move the placement table 2 to below the neck pipe 13 → clamping the assembly 5 to clamp the bucket 4 → placing the bucket 4 on the placement table 2 → loosening and taking out the clamping assembly 5 → resetting the assembly 3”, a plurality of buckets 4 containing test tubes of bovine embryos can be placed in the liquid nitrogen tank 1 for storage;

[0054] When taking out, first control the specified adjusting assembly 3 to move the corresponding placement table 2 to the directly below the neck pipe 13, control the clamping assembly 5 to clamp the bucket 4 on the placement table 2, and take out the bucket 4 from the liquid nitrogen tank 1, instead of the existing bovine embryo active preservation device using the frozen semen cloth bag storage and the rope fixing method, the situation that a plurality of ropes are wound together to cause inconvenience in taking out does not occur, and when taking out the test tube containing the bovine embryo, the staff does not need to manually take out the test tube containing the bovine embryo from the liquid nitrogen tank 1, effectively avoiding the hand frostbite of the staff and the pollution of the test tube.

[0055] It should be noted that the bucket 4 is a cylindrical structure, and has a cover 8 (not shown) on it. The cover 8 and the bucket 4 are both made of stainless steel mesh material, and are matched with the through holes 21 on the surface of the placing table 2, so that the liquid nitrogen can permeate into the bucket 4 and act on the test tubes containing the bovine embryos.

[0056] In the present application, unless otherwise explicitly specified and limited, "on" or "under" of a first feature to a second feature can include that the first and second features are in direct contact, or can include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, "on", "above" and "over" of a first feature to a second feature include that the first feature is directly above and obliquely above the second feature, or only means that the horizontal height of the first feature is higher than that of the second feature. "Under", "below" and "under" of a first feature to a second feature include that the first feature is directly below and obliquely below the second feature, or only means that the horizontal height of the first feature is less than that of the second feature.

[0057] The above shows and describes the basic principles, main features and advantages of the present application. It should be understood by those skilled in the art that the present application is not limited to the above described embodiments, and the above described embodiments and descriptions in the specification are only preferred examples of the present application, and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and all these changes and improvements fall within the scope of the present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A device for preserving viable embryos for bovine embryo transfer, characterized in that, include: A liquid nitrogen tank (1) includes an outer shell (11), an inner liner (12), and a neck tube (13). The inner liner (12) is located inside the outer shell (11). The gap between the outer shell (11) and the inner side is a sandwich (14). The neck tube (13) is installed on the top of the inner liner (12). The top end of the neck tube (13) extends through and to the top of the outer shell (11) and is provided with a cap (15). Placement platform (2), wherein several placement platforms (2) are arranged in a circular array inside the inner liner (12), and an adjustment component (3) for driving the placement platform (2) to move is installed between one side of the placement platform (2) and the inner wall of the inner liner (12). A bucket (4) is placed on a platform (2), and a clamping assembly (5) for clamping the bucket (4) is installed at the bottom of the cap (15). Each of the adjustment components (3) includes a connecting frame (31) fixed on the inner wall of the inner liner (12), a support frame (32) fixed below one end of the connecting frame (31), a rotating rod (33) rotatably connected to the inner wall of the inner liner (12), a rotating shaft A (34) rotatably connected to the inner side of the connecting frame (31), a worm gear (35) and a gear A (36) fixedly sleeved on the outer wall of the rotating shaft A (34), a worm (37) fixed at one end of the rotating rod (33), and a toothed plate (38) that slides with the support frame (32). The worm (37) meshes with the worm wheel (35), the toothed plate (38) meshes with the gear A (36), the placement platform (2) is fixed at one end of the toothed plate (38), and a guide rod A (39) is slidably connected to the support frame (32) inside the support frame (32). The two ends of the guide rod A (39) are fixed to the bottom of the toothed plate (38).

2. The embryo viability preservation device for bovine embryo transfer according to claim 1, characterized in that: The surface of the placement platform (2) is provided with several through holes (21), and several magnetic strips (22) are fixedly provided on the upper surface of the placement platform (2). A fence (23) is fixedly provided between the several magnetic strips (22). The bucket (4) is a stainless steel metal mesh material component. The bucket (4) can be locked in the fence (23) and attracted by the magnetic strips (22).

3. The embryo viability preservation device for bovine embryo transfer according to claim 1, characterized in that: One end of the rotating rod (33) passes through and extends to the outside of the outer shell (11). A sealed bearing is installed between the rotating rod (33), the outer shell (11), and the inner liner (12). A knob (310) is fixedly connected to one end of the rotating rod (33). A label is provided on the end face of the knob (310).

4. The embryo viability preservation device for bovine embryo transfer according to claim 1, characterized in that: The top and bottom of the plug (15) are respectively provided with a round hole (6) and a groove (7). The clamping assembly (5) includes a rotating shaft B (51) that is rotatably disposed in the groove (7) in the longitudinal direction, a gear B (52) and a turntable (53) that are fixedly sleeved on the outer wall of the rotating shaft B (51), a guide rod B (54) that is fixed in the groove (7) in the transverse direction, a locking rod (55) that is slidably disposed at both ends of the guide rod B (54), a connecting rod (56) that is hinged between the locking rod (55) and the turntable (53), a pressure rod (57) that is inserted in the round hole (6), a rack (58) that is fixed at the bottom end of the pressure rod (57) and meshes with the gear B (52), and a spring (59) that is sleeved on the outside of the pressure rod (57).

5. The embryo viability preservation device for bovine embryo transfer according to claim 4, characterized in that: A piston (510) is fixedly sleeved on the outer wall of the pressure rod (57). The piston (510) slides along the inner wall of the circular hole (6) and seals the circular hole (6). The spring (59) is fixed between the inner bottom wall of the circular hole (6) and the bottom of the piston (510).

6. The embryo viability preservation device for bovine embryo transfer according to claim 4, characterized in that: An annular groove (41) is provided on the upper part of the outer wall of the bucket (4), and the bottom end of the lever (55) can be inserted into the annular groove (41).

7. The embryo viability preservation device for bovine embryo transfer according to claim 4, characterized in that: The top of the outer wall of the neck tube (13) is provided with a threaded line, and the top of the neck tube (13) is threadedly connected to a cap (8) through the threaded line. The inner top wall of the cap (8) does not contact the top of the pressure rod (57).

8. The embryo viability preservation device for bovine embryo transfer according to claim 1, characterized in that: The outer shell (11), inner liner (12) and neck tube (13) are all made of hard alloy material. The inner liner (12) stores liquid nitrogen inside. The interlayer (14) is vacuum-treated and filled with heat insulation material and adsorbent.

Citation Information

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