A skin care composition for relieving infantile eczema and its preparation method

Abstract

The application provides a skin care composition for relieving infantile eczema and a preparation method thereof, and the composition comprises the following raw materials in parts by weight: aloe extract 30-50 parts, Dunaliella salina cell extract 22-38 parts, Sophora flavescens 25-35 parts, paeonol 12-18 parts, trehalose 12-18 parts and glycerol 8-16 parts; the Dunaliella salina cell extract is obtained by fermenting and culturing Dunaliella salina cells by lytic bacteria, and then performing biological crushing treatment on the obtained fermentation culture solution, adding an extraction solvent, centrifuging, collecting supernatant, concentrating and drying. The aloe extract, Dunaliella salina cell extract, Sophora flavescens, paeonol, trehalose and glycerol are scientifically proportioned to prepare the skin care composition, and the skin care composition has good anti-inflammatory effect, can improve tissue vitality, reduce the release amount of inflammatory factor IL-1alpha, and reduce the TSLP content of an epidermis model eczema caused by LPS+Poly I:C induction, and has good repair effect on the skin epidermis and certain positive curative effect on eczema treatment.

Description

Technical Field

[0001] This invention relates to the field of skin care technology, and in particular to a skin care composition for relieving eczema in infants and young children and its preparation method. Background Technology

[0002] Eczema is a common allergic and inflammatory skin disease caused by the interaction of various internal and external factors, resulting in an inflammatory reaction of the epidermis and superficial dermis. Based on the appearance of skin lesions, eczema can be divided into three stages: acute, subacute, and chronic. The chronic stage is characterized by intense itching, exudation, and frequent recurrence. With improved living standards and dietary changes, the incidence of eczema in infants and young children is rapidly increasing. Children with eczema often also have symptoms such as atopic dermatitis and allergic rhinitis, seriously affecting their health and growth. Medications used to treat eczema are mainly divided into three categories: topical corticosteroids, antihistamines, and immunomodulators. These usually have rapid effects but also have side effects. Therefore, more researchers are focusing on finding drugs with fewer side effects and better efficacy to replace these three categories. During the development of eczema, external factors can easily cause T cells to produce excessive inflammatory mediators, leading to skin inflammation and damage to the skin barrier, further aggravating the eczema reaction. Therefore, it is necessary to find a drug that can effectively improve the skin barrier function and enhance the body's protective ability to treat eczema, so as to quickly stop the progression and recurrence of eczema. Summary of the Invention

[0003] Therefore, the purpose of this invention is to provide a skin care composition for relieving infantile eczema and its preparation method.

[0004] The technical solution of this invention is implemented as follows:

[0005] A skin care composition for relieving infantile eczema, comprising, by weight, the following ingredients: 30-50 parts aloe vera extract, 22-38 parts Dunaliella salina cell extract, 25-35 parts Sophora flavescens, 12-18 parts paeoniflorin, 12-18 parts trehalose, and 8-16 parts glycerin.

[0006] Sophora flavescens Alt. is a herbaceous or subshrub belonging to the Fabaceae family and the Sophora genus. It has a bitter and cold nature, and contains non-toxic matrine and cytisine, among other substances. It possesses heat-clearing, dampness-removing, antibacterial, and anti-inflammatory properties. It can inhibit mast cell degranulation, thus exerting anti-allergic, anti-inflammatory, and antipruritic effects. Paeoniflorin, derived from the roots of Paeonia lactiflora, Paeonia suffruticosa, and Paeonia suffruticosa (Ranunculaceae family), has vasodilatory, analgesic, sedative, anti-inflammatory, anti-ulcer, antipyretic, and antispasmodic effects, and also inhibits pathogenic bacteria and fungi. Trehalose can act as a stabilizer for bioactive substances such as cell tissues, helping to improve and stabilize the bioactivity of Dunaliella salina cell extracts. Simultaneously, trehalose can also act as a skin moisturizer, nourishing skin cells and reducing the degree of skin barrier damage.

[0007] The Dunaliella salina cell extract is obtained by fermenting Dunaliella salina cells with algicidal bacteria, followed by biological disruption, adding an extraction solvent to the resulting fermentation culture broth, centrifuging, collecting the supernatant, concentrating, and drying.

[0008] The preferred formulation includes the following raw materials: 35-40 parts of aloe vera extract, 28-33 parts of Dunaliella salina cell extract, 25-30 parts of Sophora flavescens, 12-18 parts of paeoniflorin, 12-18 parts of trehalose, and 8-16 parts of glycerin.

[0009] A more preferred formulation includes the following ingredients: 38 parts aloe vera extract, 30 parts Dunaliella salina cell extract, 27 parts Sophora flavescens, 16 parts paeoniflorin, 16 parts trehalose, and 12 parts glycerin.

[0010] A more preferred embodiment is that the preparation method of the Dunaliella salina cell extract is as follows: Dunaliella salina is cultured in BG-11 medium until it reaches a stable phase. At a volume ratio of Dunaliella salina culture medium to algicidal bacteria solution of 10-12:1, algicidal bacteria solution is added. The mixture is cultured at 25-28℃ with shaking for 2-3 days. The fermentation culture medium is collected, petroleum ether is added and mixed, and the mixture is extracted with shaking for 4-8 hours. The mixture is centrifuged, the supernatant is collected, concentrated, and dried to obtain the Dunaliella salina cell extract.

[0011] A more preferred embodiment is that the viable bacteria count of the algaecide solution is ≥(1~2)×10⁻⁶. 9 per mL.

[0012] A more preferred embodiment is that the oscillation frequency is 200-250 r / min, and the volume ratio of fermentation culture medium to petroleum ether is 1-2:0.8-1.

[0013] A more preferred embodiment is Dunaliella salina, which contains active ingredients such as sporophytin and carotene, is rich in oils, and contains a variety of essential amino acids. It has excellent antioxidant properties and is also effective in repairing damaged skin.

[0014] The present invention also provides a method for preparing the above-mentioned skin care composition for relieving infantile eczema, comprising the following steps:

[0015] S1 Take Sophora flavescens powder, add water and decoct, filter to obtain Sophora flavescens liquid;

[0016] S2 Take aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract, add water, and sonicate to stir to obtain active ingredient solution;

[0017] S3 adds paeoniflorin, trehalose, and glycerin to the active ingredient solution to obtain the skin care composition.

[0018] The preferred embodiment is that, in step S1, the mass ratio of the Sophora flavescens to water is 1:6 to 8; and in step S2, the amount of water added is 2 to 5 times the weight of the material.

[0019] The preferred embodiment is that the power of the ultrasound is 600-800W and the duration of the ultrasound is 30-40 minutes.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] This invention utilizes aloe vera extract, Dunaliella salina cell extract, Sophora flavescens, paeoniflorin, trehalose, and glycerin in a scientifically formulated ratio to create a skincare composition with good anti-inflammatory effects. This composition enhances tissue vitality, reduces the release of the inflammatory factor IL-1α, and decreases LPS+Poly I:C-induced inflammation. The TSLP content in the epidermal model of eczema has a good repair effect on the skin epidermis and has a certain positive therapeutic effect on eczema.

[0022] The skin care composition for relieving infantile eczema provided by this invention is mild in nature and can be used for daily skin care. It helps to improve the skin barrier function, enhance the body's protective ability, improve the skin's resistance to external substances and pathogens, further reduce and prevent skin inflammation and barrier damage, and avoid recurrent eczema. Detailed Implementation

[0023] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0024] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0025] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0026] Aloe vera extract was purchased from Shanghai Yuanye Biotechnology Co., Ltd., Dunaliella salina was purchased from Tianjin Changlu Hangu Saltworks Co., Ltd., Dunaliella salina extract (also known as Dunaliella salina powder) was purchased from Xi'an Wenzhu Biotechnology Co., Ltd., Brevibacillus laterosporus was purchased from Jiangsu Lvke Biotechnology Co., Ltd., and the 3D epidermal skin model Epikutis was purchased from Guangdong Boxi Biotechnology Co., Ltd.

[0027] BG-11 culture medium formula: NaNO3 1.5g / L, K2HPO4 0.04g / L, MgSO4·7H2O 0.075g / L, CaCl2·7H2O 0.36g / L, Na2CO3 0.02g / L, sodium citrate 0.006g / L, H3BO4 2.86g / L, MnCl2·4H2O 1.81g / L, ZnSO4 0.222g / L, Na2MnO4 0.39g / L, CuSO4·5H2O 0.079g / L, Co(NO3)2·6H2O 0.494g / L.

[0028] Example 1

[0029] A skin care composition for relieving infantile eczema, with the following formula: 38 parts aloe vera extract, 30 parts Dunaliella salina cell extract, 27 parts sophora flavescens, 16 parts paeoniflorin, 16 parts trehalose, and 12 parts glycerin.

[0030] The preparation method of Dunaliella salina cell extract is as follows: Dunaliella salina is first inoculated into BG-11 solid medium and streaked for 2-3 generations to obtain purified Dunaliella salina. Single colonies with neat edges are then inoculated into BG-11 liquid medium and cultured at 25-28℃ with a light intensity of 12000 lx and a photoperiod of 12 h / d. When Dunaliella salina reaches the cell stationary phase, 1×10⁶ viable bacteria are added at a volume ratio of 12:1. 10 The algae-lysing bacterial solution was cultured at 25–28°C and 200–250 rpm for 3 days. The fermentation broth was collected and mixed with petroleum ether at a volume ratio of 1:0.8. After extraction by shaking for 5 hours, the mixture was centrifuged, the supernatant was collected, concentrated, and freeze-dried to obtain Dunaliella salina cell extract.

[0031] The preparation steps are as follows:

[0032] S1 According to the formula, take Sophora flavescens powder, crush it, add water with 7 times the weight of Sophora flavescens powder, decoct it, filter it, and obtain Sophora flavescens liquid.

[0033] S2 Take aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract, add water with 2 times the weight of the materials (aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract), and ultrasonically stir at 700W for 40 minutes to obtain active ingredient solution.

[0034] S3 adds paeoniflorin, trehalose, and glycerin to the active ingredient solution to obtain the target skin care composition.

[0035] Example 2

[0036] A skin care composition for relieving infantile eczema, with the following formula: 50 parts aloe vera extract, 38 parts Dunaliella salina cell extract, 35 parts sophora flavescens, 18 parts paeoniflorin, 16 parts trehalose and 12 parts glycerin.

[0037] The preparation method of Dunaliella salina cell extract is as follows: Dunaliella salina is first inoculated into BG-11 solid medium and streaked for 2-3 generations to obtain purified Dunaliella salina. Single colonies with neat edges are then inoculated into BG-11 liquid medium and cultured at 25-28℃ with a light intensity of 12000 lx and a photoperiod of 12 h / d. When Dunaliella salina reaches the cell stationary phase, 1×10⁶ viable bacteria are added at a volume ratio of 12:1. 10 The algae-lysing bacterial solution was cultured at 25–28°C and 200–250 rpm for 3 days. The fermentation broth was collected and mixed with petroleum ether at a volume ratio of 1:0.8. After extraction by shaking for 5 hours, the mixture was centrifuged, the supernatant was collected, concentrated, and freeze-dried to obtain Dunaliella salina cell extract.

[0038] The preparation steps are as follows:

[0039] S1 According to the formula, take Sophora flavescens powder, crush it, add water with 7 times the weight of Sophora flavescens powder, decoct it, filter it, and obtain Sophora flavescens liquid.

[0040] S2 Take aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract, add 5 times the weight of water to the materials (aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract), and ultrasonically stir at 700W for 40 minutes to obtain the active ingredient solution.

[0041] S3 adds paeoniflorin, trehalose, and glycerin to the active ingredient solution to obtain the target skin care composition.

[0042] Example 3

[0043] A skin care composition for relieving infantile eczema, with the following formula: 40 parts aloe vera extract, 33 parts Dunaliella salina cell extract, 30 parts sophora flavescens, 18 parts paeoniflorin, 16 parts trehalose, and 12 parts glycerin.

[0044] The preparation method of Dunaliella salina cell extract is as follows: Dunaliella salina is first inoculated into BG-11 solid medium and streaked for 2-3 generations to obtain purified Dunaliella salina. Single colonies with neat edges are then inoculated into BG-11 liquid medium and cultured at 25-28℃ with a light intensity of 12000 lx and a photoperiod of 12 h / d. When Dunaliella salina reaches the cell stationary phase, 1×10⁶ viable bacteria are added at a volume ratio of 12:1. 10 The algae-lysing bacterial solution was cultured at 25–28°C and 200–250 rpm for 3 days. The fermentation broth was collected and mixed with petroleum ether at a volume ratio of 1:0.8. After extraction by shaking for 5 hours, the mixture was centrifuged, the supernatant was collected, concentrated, and freeze-dried to obtain Dunaliella salina cell extract.

[0045] The preparation steps are as follows:

[0046] S1 According to the formula, take Sophora flavescens powder, crush it, add water with 7 times the weight of Sophora flavescens powder, decoct it, filter it, and obtain Sophora flavescens liquid.

[0047] S2 Take aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract, add water with 3 times the weight of the materials (aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract), and ultrasonically stir at 700W for 40 minutes to obtain active ingredient solution.

[0048] S3 adds paeoniflorin, trehalose, and glycerin to the active ingredient solution to obtain the target skin care composition.

[0049] Comparative Example 1

[0050] The difference between this comparative example and Example 1 is that the amount of Dunaliella salina cell extract and paeoniflorin in the formula is adjusted. The formula is as follows: 38 parts of aloe vera extract, 10 parts of Dunaliella salina cell extract, 27 parts of Sophora flavescens, 25 parts of paeoniflorin, 16 parts of trehalose and 12 parts of glycerin.

[0051] The preparation method of Dunaliella salina cell extract is as follows: Dunaliella salina is first inoculated into BG-11 solid medium and streaked for 2-3 generations to obtain purified Dunaliella salina. Single colonies with neat edges are then inoculated into BG-11 liquid medium and cultured at 25-28℃ with a light intensity of 12000 lx and a photoperiod of 12 h / d. When Dunaliella salina reaches the cell stationary phase, 1×10⁶ viable bacteria are added at a volume ratio of 12:1. 10 The algae-lysing bacterial solution was cultured at 25–28°C and 200–250 rpm for 3 days. The fermentation broth was collected and mixed with petroleum ether at a volume ratio of 1:0.8. After extraction by shaking for 5 hours, the mixture was centrifuged, the supernatant was collected, concentrated, and freeze-dried to obtain Dunaliella salina cell extract.

[0052] The preparation steps are as follows:

[0053] S1 According to the formula, take Sophora flavescens powder, crush it, add water with 7 times the weight of Sophora flavescens powder, decoct it, filter it, and obtain Sophora flavescens liquid.

[0054] S2 Take aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract, add water with 2 times the weight of the materials (aloe vera extract, Dunaliella salina cell extract and Sophora flavescens extract), and ultrasonically stir at 700W for 40 minutes to obtain active ingredient solution.

[0055] S3 adds paeoniflorin, trehalose, and glycerin to the active ingredient solution to obtain the target skin care composition.

[0056] Comparative Example 2

[0057] The difference between this comparative example and Example 1 is that commercially available Dunaliella salina extract is used to replace Dunaliella salina cell extract, and the ingredients in the formula are adjusted. The formula of this skin care composition is as follows: 38 parts aloe vera extract, 30 parts Dunaliella salina extract, 27 parts Sophora flavescens, 16 parts camellia oil, 16 parts β-glucan and 12 parts glycerin.

[0058] The preparation steps are as follows:

[0059] S1 According to the formula, take Sophora flavescens powder, crush it, add water with 7 times the weight of Sophora flavescens powder, decoct it, filter it, and obtain Sophora flavescens liquid.

[0060] S2 Take aloe vera extract, Dunaliella salina extract and Sophora flavescens extract, add water with 2 times the weight of the materials (aloe vera extract, Dunaliella salina extract and Sophora flavescens extract), and ultrasonically stir at 700W for 40 minutes to obtain active ingredient solution.

[0061] S3 adds camellia oil, β-glucan, glycerin and an appropriate amount of emulsifier to the active ingredient solution, and then shears to obtain the target skin care composition.

[0062] Comparative Example 3

[0063] The difference between this comparative example and Example 1 is that hydrolyzed Euglena extract is used instead of Dunaliella salina cell extract. The formula of this skin care composition is as follows: 38 parts aloe vera extract, 30 parts hydrolyzed Euglena extract, 27 parts sophora flavescens, 16 parts paeoniflorin, 16 parts trehalose and 12 parts glycerin.

[0064] The preparation method of hydrolyzed Euglena extract is as follows: Euglena is taken, crushed, and 10 times its weight of water is added. After soaking for 2 hours, it is ultrasonically broken for 20 minutes, centrifuged, and the supernatant is collected. Ammonium sulfate is added and stirred for 1 hour. The mixture is allowed to stand overnight at 4°C, centrifuged, and the precipitate is collected. The precipitate is dissolved and dialyzed. The mixture is then freeze-dried to obtain hydrolyzed Euglena extract.

[0065] The preparation steps are as follows:

[0066] S1 According to the formula, take Sophora flavescens powder, crush it, add water with 7 times the weight of Sophora flavescens powder, decoct it, filter it, and obtain Sophora flavescens liquid.

[0067] S2 Take aloe vera extract, hydrolyzed Euglena gracilis extract and Sophora flavescens extract, add water twice the weight of the materials (aloe vera extract, hydrolyzed Euglena gracilis extract and Sophora flavescens extract), and ultrasonically stir at 700W for 40 minutes to obtain the active ingredient solution.

[0068] S3 adds paeoniflorin, trehalose, and glycerin to the active ingredient solution to obtain the target skin care composition.

[0069] (1) Anti-inflammatory efficacy test

[0070] The in vitro anti-eczema and anti-inflammatory effects of skincare compositions were studied using the Epikutis 3D epidermal skin model. The method was as follows: 0.9 mL of TA culture medium was added to a 6-well plate. The 3D skin model was then added to the 6-well plate. Each group of skincare composition and 25 μL of sodium dodecyl sulfate (SLS) at a concentration of 0.002 g / mL were added evenly to the model. The positive group received 0.05% dexamethasone, the negative group received only SLS, and the blank group received no treatment except for an equal volume of TA culture medium. The plates were incubated at 37℃ and 5% CO2 for 24 h. After incubation, the culture medium was collected, and the IL-1α content was measured using an ELISA kit. The model was then removed, its surface was cleaned with sterile PBS solution, and the liquid inside and outside the model was absorbed with sterile cotton. The model was then transferred to a 24-well plate, and 1 mg / mL of TA culture medium was added evenly. 3-(4,5-Dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) was incubated at 37°C and 5% CO2 for 3 hours. The model was then removed, and the MTT inside and outside the model was absorbed with sterile cotton. The mixture was transferred to a 24-well plate, isopropanol was added evenly, the plate was sealed, and the plate was incubated overnight at 4°C. The model was then punctured, the culture medium was collected, and the mixture was thoroughly agitated. The absorbance was measured at 570 nm, and tissue viability was calculated. The results are as follows.

[0071] Table 1. Results of tissue viability and IL-1α levels in each group.

[0072]

[0073]

[0074] The results in the table above show that, compared with the negative group, the positive group exhibited increased tissue vitality and significantly decreased IL-1α levels, indicating that the in vitro positive detection of this model is effective. Furthermore, the tissue vitality of Examples 1-3 was superior to that of the negative group and Comparative Examples 1-3, while the IL-1α content was reduced. This demonstrates that the skincare composition prepared by scientifically proportioning aloe vera extract, Dunaliella salina cell extract, Sophora flavescens, paeoniflorin, trehalose, and glycerin has good anti-inflammatory effects, enhances tissue vitality, and reduces the release of inflammatory factors.

[0075] (2) Anti-eczema efficacy test

[0076] Add 0.9 mL of TA culture medium to a 6-well plate, add the 3D skin model to the 6-well plate, and evenly add 25 μL of each group of skin care composition to the model. Incubate at 37℃, 5% CO2, and 95% relative humidity for 24 h. After absorbing the liquid inside and outside the model with sterile cotton and discarding the culture medium, add lipopolysaccharide (LPS) + polyinosinic acid (Poly I:C) induction solution (LPS concentration 20 μg / mL + Poly I:C concentration 24 μg / mL). The positive group is treated with 0.05% dexamethasone, the negative group is treated with only LPS + Poly I:C, and the blank group is treated with no treatment except adding an equal volume of TA culture medium. Incubate for another 24 h at 37℃, 5% CO2, and 95% relative humidity. Collect the culture medium and use an ELISA kit to determine the TSLP content. The results are as follows.

[0077] Table 2 shows the results of TSLP content in each group.

[0078] Group <![CDATA[TSLP / pg·mL -1 ]]> Example 1 36.85 Example 2 34.27 Example 3 36.22 Comparative Example 1 45.35 Comparative Example 2 48.76 Comparative Example 3 53.66 positive group 45.12 negative group 72.43 Blank group 22.16

[0079] The results in the table above show that the TSLP content in the positive group was significantly lower than that in the negative group, indicating that the in vitro positive detection of this model is effective. Furthermore, the degree of TSLP content reduction in Examples 1-3 was superior to that in the positive group and Comparative Examples 1-3, demonstrating that the skincare composition prepared by scientifically proportioning aloe vera extract, Dunaliella salina cell extract, Sophora flavescens, paeoniflorin, trehalose, and glycerin can reduce LPS+Poly I:C-induced TSLP levels. The TSLP content in the epidermal model of eczema has a good repair effect on the skin epidermis and has a certain positive therapeutic effect on eczema. Paeoniflorin, derived from the roots of Paeonia lactiflora, Paeonia suffruticosa, and Paeonia suffruticosa, has the effects of vasodilating, analgesic, sedative, anti-inflammatory, anti-ulcer, antipyretic, and antispasmodic, and has an inhibitory effect on pathogenic bacteria and fungi. Trehalose can act as a stabilizer for bioactive substances such as cell tissues, helping to improve and stabilize the bioactivity of Dunaliella salina cell extract. At the same time, trehalose can also act as a skin epidermal moisturizer, moisturizing skin cells and reducing the degree of skin barrier damage. The addition of Dunaliella salina cell extract, Sophora flavescens, paeoniflorin, and trehalose can have a synergistic effect with aloe vera extract to quickly repair the damaged skin barrier and shorten the course of treatment.

[0080] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skin care composition for relieving infantile eczema, characterized by, The skin care composition comprises the following raw materials by weight: aloe extract 30-50 parts, Dunaliella salina cell extract 22-38 parts, Sophora flavescens 25-35 parts, paeoniflorin 12-18 parts, trehalose 12-18 parts, and glycerol 8-16 parts. The Dunaliella salina cell extract is prepared by the following method: Dunaliella salina is cultured in BG-11 medium until it is in the stable phase, Dunaliella salina culture solution and algae-lysing bacteria solution are mixed at a volume ratio of 10-12:1, the mixture is cultured at 25-28℃ for 2-3 days, the fermentation culture solution is collected, petroleum ether is added, the mixture is extracted by oscillation for 4-8 hours, the supernatant is collected by centrifugation, the supernatant is concentrated and dried to obtain the Dunaliella salina cell extract. The saltwort is Duval saltwort (Dunaliella salina) Dunaliella salina ).

2. The skin care composition for alleviating eczema of infant's skin according to claim 1, wherein The skin care composition comprises the following raw materials: aloe extract 35-40 parts, Dunaliella salina cell extract 28-33 parts, Sophora flavescens 25-30 parts, paeoniflorin 12-18 parts, trehalose 12-18 parts, and glycerol 8-16 parts.

3. The skin care composition for alleviating eczema of infant's skin according to claim 2, characterized by, The skin care composition comprises the following raw materials: aloe extract 38 parts, Dunaliella salina cell extract 30 parts, Sophora flavescens 27 parts, paeoniflorin 16 parts, trehalose 16 parts, and glycerol 12 parts.

4. The skin care composition for alleviating eczema of infant's skin according to claim 1, wherein The viable cell count of the algicidal bacteria liquid is ≥1×10 9 / mL.

5. The skin care composition for alleviating eczema of infant's skin according to claim 1, wherein The frequency of oscillation is 200-250 r / min, and the volume ratio of fermentation culture solution to petroleum ether is 1-2:0.8-1.

6. The method for preparing the skin care composition for relieving infantile eczema according to any one of claims 1 to 5, characterized in that, The method comprises the following steps: S1: Sophora flavescens is ground and boiled in water, and the mixture is filtered to obtain Sophora flavescens liquid; S2: aloe extract, Dunaliella salina cell extract, and Sophora flavescens liquid are added to water, and the mixture is stirred by ultrasonic to obtain active ingredient liquid; S3: paeoniflorin, trehalose, and glycerol are added to the active ingredient liquid to obtain the skin care composition.

7. The method of preparing a skin care composition for alleviating eczema in the skin of an infant according to claim 6, characterized in that, In step S1, the mass ratio of Sophora flavescens to water is 1:6-8; in step S2, the amount of water added is 2-5 times the weight of the materials.

8. The method of preparing a skin care composition for alleviating eczema in the skin of an infant according to claim 6, characterized in that, The power of ultrasonic is 600-800 W, and the time of ultrasonic is 30-40 min.

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