An essential oil for weight loss, its preparation method and application

Abstract

The present invention relates to the field of pharmaceutical technology, and specifically discloses an essential oil for weight loss, its preparation method and application. The essential oil comprises the following components in volume parts: 9-20 parts of Fructus Aurantii Immaturus essential oil, 12-27 parts of Atractylodes Macrocephala essential oil and a carrier essential oil; the volume ratio of the carrier essential oil to the sum of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil is 3:11-17. Through the synergistic effect of specific volume parts of Fructus Aurantii Immaturus and Atractylodes Macrocephala, the essential oil can be used for obese people and has a significant weight loss effect; the essential oil has a significant moisture-lowering effect and has no irritation and sensitivity to the skin; the essential oil can be taken orally and externally, does not produce side effects on the human body, and has high safety; moreover, the preparation method of the essential oil is simple and the price is low, and it has broad market application prospects.

Description

Technical Field

[0001] The present invention belongs to the field of pharmaceutical technology, and particularly relates to an essential oil for weight loss, a preparation method thereof and an application thereof. Background Art

[0002] Obesity is one of the most common metabolic diseases. Generally, when energy intake is higher than energy consumption, the human body will gain weight. When energy intake and consumption are unbalanced for a long time, it will stimulate energy to be stored in fat cells in the form of triglycerides. When the fat storage exceeds the physiological normal value, obesity can occur. Long-term obesity can induce chronic non-communicable diseases such as hypertension, cardiovascular and cerebrovascular diseases, diabetes and tumors.

[0003] There are many existing weight loss methods. Although diet-induced weight loss can effectively reduce weight, the sharp reduction in intake leads to nutritional deficiencies, a decrease in resting metabolic rate, and weight regain after stopping diet intervention. Exercise-induced weight loss can significantly reduce abdominal obesity, but it also affects cardiopulmonary health, and improper exercise will increase the risk of musculoskeletal injuries. Surgical operation is the most effective method, which has an impact on obesity complications, but its standards are not unified, and there are individual differences, and the reaction to the operation cannot be predicted. Currently marketed weight loss drugs mainly include liraglutide, orlistat, phentermine, cloxacillin, phentermine-topiramate, but these synthetic drugs often have strong toxic and side effects, and long-term use will make patients develop dependence and tolerance. Therefore, exploring obesity therapies from the perspective of traditional Chinese medicine combined with Western medicine mechanisms can enrich the treatment ideas for obesity and make up for the deficiencies in modern Western medicine treatment.

[0004] Chinese Patent Application CN105853877A discloses a pharmaceutical composition for weight loss, its preparation and its preparation method, belonging to the field of pharmaceutical technology. The active ingredients of the pharmaceutical composition for weight loss include Poria cocos, Atractylodes macrocephala, Pinellia ternata, Immature Bitter Orange, Eupatorium fortunei, Atractylodes lancea, Magnolia officinalis, Rheum officinale, Bupleurum chinense, Amomum tsao-ko, Lotus Leaf, Lindera aggregata, Cinnamon, Safflower, Angelica sinensis, White Peony Root, Chuanxiong Rhizome, Sichuan Pepper, Astragalus membranaceus, Alisma orientale, Amomum villosum, Capsaicin. This invention provides a new way for the treatment of obesity, especially abdominal obesity. The pharmaceutical composition for weight loss and its preparation disclosed in this invention can remove turbidity and fullness, and prevent and treat obesity, especially the complications of abdominal obesity by improving fat metabolism, and has an obvious improvement effect on abdominal fat accumulation. Another example is Chinese Patent Application CN112717012A, which provides a weight loss compound essential oil, its preparation method and application. The weight loss compound essential oil is composed of Immature Bitter Orange essential oil, Atractylodes macrocephala essential oil, Magnolia officinalis essential oil and base oil. The formula is reasonable, has good weight loss efficacy, uses natural products, can give full play to the synergistic effect between effective ingredients, fundamentally improves human body functions, accelerates metabolism, accelerates fat burning, and achieves a good weight loss effect. At the same time, the usage method is safe and comfortable, with small side effects, greatly reducing the pain of obese people during the weight loss process.

[0005] However, the raw materials of the above compositions are numerous, the preparation is cumbersome, and the price is relatively expensive. Therefore, there is an urgent need for a weight-loss essential oil that is simple to prepare, low in price, has significant weight-loss and dampness-reducing effects, and can be taken orally and topically. Summary of the Invention

[0006] Unless the context clearly indicates otherwise, as used herein, the singular forms "a", "an", and "the" include both singular and plural referents. A numerical range expressed by endpoints includes all the values and fractions within the corresponding range, as well as the expressed endpoints.

[0007] In view of the problems existing in the prior art, the present invention provides an essential oil for weight loss, its preparation method and application. Through the synergistic effect of the medicine and food homologous herbs, Fructus Aurantii Immaturus and Atractylodes Macrocephala, this essential oil can be used for obese people, has a significant dampness-reducing effect, and can be taken orally and topically.

[0008] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] On the one hand, the present invention provides an essential oil for weight loss, comprising the following components in volume parts: 9-20 parts of Fructus Aurantii Immaturus essential oil, 12-27 parts of Atractylodes Macrocephala essential oil, and a carrier essential oil; the volume ratio of the carrier essential oil to the sum of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil is 3:11-17.

[0010] Preferably, the volume ratio of the Fructus Aurantii Immaturus essential oil to the Atractylodes Macrocephala essential oil is 3-6:8-9.

[0011] Preferably, the carrier essential oil includes at least one of Amomum villosum essential oil, Aucklandia lappa essential oil, Cinnamomum cassia essential oil, Citrus reticulata Blanco essential oil, Citrus aurantium L. var. amara Engl. essential oil, Citrus medica L. var. sarcodactylis Swingle essential oil, Eugenia caryophyllata Thunb. essential oil, Pogostemon cablin essential oil, Zanthoxylum bungeanum Maxim. essential oil, Citrus reticulata Blanco cv. Qingpi essential oil, Myristica fragrans Houtt. essential oil, Perilla frutescens essential oil, Citrus reticulata Blanco essential oil, Foeniculum vulgare Mill. essential oil, Atractylodes lancea essential oil, Artemisia capillaris Thunb. essential oil, Boswellia carterii Birdw. essential oil, Zingiber officinale Roscoe essential oil, and Citrus aurantium L. essential oil.

[0012] More preferably, the carrier essential oil includes Amomum villosum essential oil, Perilla frutescens essential oil, Cinnamomum cassia essential oil, Citrus reticulata Blanco essential oil, and Citrus aurantium L. essential oil.

[0013] Preferably, the volume ratio of the carrier essential oil to the sum of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil is 3:11-15.

[0014] More preferably, the volume ratio of the carrier essential oil to the sum of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil is 3:15.

[0015] The present invention also provides a preparation method of the above essential oil, which is characterized by comprising the following steps:

[0016] S1. Mix the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil evenly according to the required ratio;

[0017] S2. Add the remaining carrier essential oil to the mixed essential oil obtained in step S1, mix evenly, and then divide and package to obtain the product.

[0018] Preferably, the preparation methods of the Fructus Aurantii Immaturus essential oil and the Atractylodes macrocephala essential oil include the following steps:

[0019] S1. Pretreatment: Air-dry and crush Fructus Aurantii Immaturus and Atractylodes macrocephala respectively, and sieve through an 80-100 mesh sieve;

[0020] S2. Soaking: Soak the crushed Fructus Aurantii Immaturus and Atractylodes macrocephala in step S1 with double-distilled water to obtain a Fructus Aurantii Immaturus mixture and an Atractylodes macrocephala mixture;

[0021] S3. Distillation: Perform distillation treatment on the Fructus Aurantii Immaturus mixture and the Atractylodes macrocephala mixture in step S2 respectively;

[0022] S4. Drying: Use anhydrous sodium sulfate to perform drying treatment on the distilled Fructus Aurantii Immaturus fraction and Atractylodes macrocephala fraction respectively to obtain Fructus Aurantii Immaturus essential oil and Atractylodes macrocephala essential oil.

[0023] Preferably, in the soaking in step S2, the material-liquid ratio of Fructus Aurantii Immaturus / Atractylodes macrocephala to double-distilled water is 1 g: 5-7 ml; the conditions for the soaking in step S2 are: temperature 15-30 °C, time 40-80 min; the conditions for the distillation in step S3 are: temperature 80-90 °C, pressure 300-350 mbar, time 5-6 h.

[0024] On the other hand, the present invention provides an application of the above essential oil or the essential oil prepared by the above preparation method in the preparation of a preparation for treating human obesity.

[0025] Preferably, the preparation includes an oral preparation and a non-oral administration preparation; the oral preparation includes microcapsules, suspensions, dripping pills, and oral liquids; the non-oral administration preparation includes inhalants, aerosols, and transdermal medicaments.

[0026] The present invention also provides an oral preparation prepared by the above essential oil.

[0027] The present invention also provides a non-oral preparation prepared by the above essential oil.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) Based on the synergistic effect between Fructus Aurantii Immaturus and Atractylodes macrocephala which are both medicine and food, the essential oil prepared has a significant weight loss effect on obese people. Because the essential oil has a small molecular weight and is easily absorbed by the human body, it can accelerate blood circulation, promote body metabolism, enhance lymphocyte detoxification, and promote fat consumption;

[0030] (2) Through the synergistic effect of a specific volume fraction of Fructus Aurantii Immaturus and Atractylodes macrocephala Koidz. and the carrier essential oil, the essential oil of the present invention can significantly reduce the dampness in the human body, and has no irritation and sensitivity to the skin after use; the essential oil can be taken orally and externally, will not cause side effects to the human body, and has high safety;

[0031] (3) The essential oil of the present invention is only prepared from common Chinese herbs with both medicinal and edible uses, the raw materials are simple and easy to obtain, the price is low, and the preparation process is simple. Detailed implementation manners

[0032] The present invention will be described below through specific examples to make the technical solutions of the present invention easier to understand and master, but the present invention is not limited thereto. The described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0033] In the ranges disclosed herein, the endpoints and any values are not limited to the exact ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, between the endpoint values of each range, between the endpoint values of each range and a single point value, and between single point values, they can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed herein.

[0034] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. The experimental methods described in the following embodiments are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, can all be obtained from commercial channels.

[0035] Example 1

[0036] An essential oil for weight loss, and its preparation method includes the following steps:

[0037] Take 12 ml of Fructus Aurantii Immaturus essential oil and 18 ml of Atractylodes macrocephala Koidz. essential oil and mix them evenly; then add 6 ml of carrier essential oil (the volume ratio of Amomum villosum Lour. essential oil, Perilla frutescens (L.) Britt. essential oil, Cinnamomum cassia Presl essential oil, Citrus reticulata Blanco essential oil, Fructus Aurantii essential oil is 1:2:2:0.5:0.5), and after mixing evenly, subpackage to obtain.

[0038] Among them, the preparation method of Fructus Aurantii Immaturus essential oil includes the following steps:

[0039] S1. Pretreatment: Dry the Fructus Aurantii Immaturus and then crush it, and pass through a 90-mesh sieve;

[0040] S2. Soaking: Soak the crushed Fructus Aurantii Immaturus in double-distilled water at 25 °C for 50 min to obtain a Fructus Aurantii Immaturus mixture, where the solid-liquid ratio is 1 g:6 ml;

[0041] S3. Distillation: Distill the soaked Fructus Aurantii Immaturus mixture in step S2. The distillation conditions are as follows: temperature 85°C, pressure 300 mbar, and time 5.5 h;

[0042] S4. Drying: Use anhydrous sodium sulfate to dehydrate and dry the distilled Fructus Aurantii Immaturus fraction to obtain Fructus Aurantii Immaturus essential oil.

[0043] The preparation methods of Atractylodes macrocephala Koidz., Amomum villosum Lour., Perilla frutescens (L.) Britt., Cinnamomum cassia Presl, Citrus reticulata Blanco and Fructus Aurantii essential oils are the same as that of Fructus Aurantii Immaturus essential oil.

[0044] Example 2

[0045] An essential oil for weight loss, the preparation method of which comprises the following steps: Take 9 ml of Fructus Aurantii Immaturus essential oil and 24 ml of Atractylodes macrocephala Koidz. essential oil and mix them evenly; then add 9 ml of carrier essential oil (the volume ratio of Citrus reticulata Blanco var. tangerina essential oil, Pogostemon cablin essential oil, Myristica fragrans Houtt. essential oil, Aucklandia lappa Decne. essential oil, Citrus medica L. var. sarcodactylis essential oil is 1:2:2:0.5:0.5), and after mixing evenly, divide and pack to obtain.

[0046] Among them, the preparation method of Fructus Aurantii Immaturus essential oil comprises the following steps:

[0047] S1. Pretreatment: Air-dry the Fructus Aurantii Immaturus and then crush it, and pass through a 100-mesh sieve;

[0048] S2. Soaking: Soak the crushed Fructus Aurantii Immaturus in double-distilled water at 15°C for 80 min to obtain a Fructus Aurantii Immaturus mixture, where the solid-liquid ratio is 1 g:7 ml;

[0049] S3. Distillation: Distill the soaked Fructus Aurantii Immaturus mixture in step S2. The distillation conditions are as follows: temperature 80°C, pressure 350 mbar, and time 6 h;

[0050] S4. Drying: Use anhydrous sodium sulfate to dehydrate and dry the distilled Fructus Aurantii Immaturus fraction to obtain Fructus Aurantii Immaturus essential oil.

[0051] The preparation methods of Atractylodes macrocephala Koidz., Citrus reticulata Blanco var. tangerina, Pogostemon cablin, Myristica fragrans Houtt., Aucklandia lappa Decne. and Citrus medica L. var. sarcodactylis essential oils are the same as that of Fructus Aurantii Immaturus essential oil.

[0052] Example 3

[0053] An essential oil for weight loss, the preparation method of which comprises the following steps: Take 9 ml of Fructus Aurantii Immaturus essential oil and 27 ml of Atractylodes macrocephala Koidz. essential oil and mix them evenly; then add 9 ml of carrier essential oil (the volume ratio of Citrus aurantium L. var. amara Engl. essential oil, Eugenia caryophyllata Thunb. essential oil, Citrus reticulata Blanco essential oil, Foeniculum vulgare Mill. essential oil, Zanthoxylum bungeanum Maxim. essential oil is 1:2:2:0.5:0.5), and after mixing evenly, divide and pack to obtain.

[0054] Among them, the preparation method of Fructus Aurantii Immaturus essential oil comprises the following steps:

[0055] S1. Pretreatment: Air-dry the Fructus Aurantii Immaturus and then crush it, and pass through an 80-mesh sieve;

[0056] S2. Soaking: Soak the crushed Fructus Aurantii Immaturus in double-distilled water at 30 °C for 40 min to obtain a Fructus Aurantii Immaturus mixture, where the solid-liquid ratio is 1 g: 5 ml;

[0057] S3. Distillation: Distill the soaked Fructus Aurantii Immaturus mixture in step S2, where the distillation conditions are: temperature 90 °C, pressure 330 mbar, and time 5 h;

[0058] S4. Drying: Use anhydrous sodium sulfate to dehydrate and dry the distilled Fructus Aurantii Immaturus fraction to obtain Fructus Aurantii Immaturus essential oil.

[0059] The preparation methods of Atractylodes macrocephala Koidz., Citrus aurantium L. var. amara Engl., Eugenia caryophyllata Thunb., Pericarpium Citri Reticulatae, Foeniculum vulgare Mill., and Zanthoxylum bungeanum Maxim. essential oils are the same as that of Fructus Aurantii Immaturus essential oil.

[0060] Example 4

[0061] An essential oil for weight loss, and its preparation method includes the following steps:

[0062] Take 12 ml of Fructus Aurantii Immaturus essential oil and 18 ml of Atractylodes macrocephala Koidz. essential oil and mix them evenly; then add 6 ml of carrier essential oil (the volume ratio of Atractylodes lancea (Thunb.) DC., Perilla frutescens (L.) Britt., Artemisia capillaris Thunb., Boswellia carterii Birdw., and Zingiber officinale Roscoe essential oils is 1:1:1:1:1), and after mixing evenly, divide and package to obtain.

[0063] Among them, the preparation method of Fructus Aurantii Immaturus essential oil includes the following steps:

[0064] S1. Pretreatment: Air-dry and crush Fructus Aurantii Immaturus, and pass through a 90-mesh sieve;

[0065] S2. Soaking: Soak the crushed Fructus Aurantii Immaturus in step S1 in double-distilled water at 25 °C for 50 min to obtain a Fructus Aurantii Immaturus mixture, where the solid-liquid ratio is 1 g: 6 ml;

[0066] S3. Distillation: Distill the soaked Fructus Aurantii Immaturus mixture in step S2, where the distillation conditions are: temperature 85 °C, pressure 300 mbar, and time 5.5 h;

[0067] S4. Drying: Use anhydrous sodium sulfate to dehydrate and dry the distilled Fructus Aurantii Immaturus fraction to obtain Fructus Aurantii Immaturus essential oil.

[0068] The preparation methods of Atractylodes macrocephala Koidz., Atractylodes lancea (Thunb.) DC., Perilla frutescens (L.) Britt., Artemisia capillaris Thunb., Boswellia carterii Birdw., and Zingiber officinale Roscoe essential oils are the same as that of Fructus Aurantii Immaturus essential oil.

[0069] Comparative Example 1

[0070] An essential oil for weight loss. Its preparation method is different from that of Example 1 only in that: take 15 ml of Fructus Aurantii Immaturus essential oil and 50 ml of Atractylodes Macrocephala essential oil, mix them evenly; then add 13 ml of carrier essential oil (the volume ratio of Amomum Villosum essential oil, Perilla Frutescens essential oil, Cinnamomum Cassia essential oil, Citrus Reticulata Blanco essential oil, and Fructus Aurantii essential oil is 1:2:2:0.5:0.5), and after mixing evenly, divide and package to obtain it.

[0071] The preparation methods of Fructus Aurantii Immaturus, Atractylodes Macrocephala, Amomum Villosum, Perilla Frutescens, Cinnamomum Cassia, Citrus Reticulata Blanco, and Fructus Aurantii essential oils are the same as those in Example 1.

[0072] Comparative Example 2

[0073] A medicinal liquid for weight loss, including the following steps: weigh 15 g of Fructus Aurantii Immaturus and 6 g of Atractylodes Macrocephala, place them in a casserole, add 500 ml of water and boil for 1 hour, and filter out the medicinal residues to obtain the medicinal liquid.

[0074] Comparative Example 3

[0075] An essential oil for weight loss. Its preparation method is different from that of Example 1 only in that: take 12 ml of Fructus Aurantii Immaturus essential oil and 18 ml of Atractylodes Macrocephala essential oil, mix them evenly and divide and package to obtain it. The preparation methods of Fructus Aurantii Immaturus and Atractylodes Macrocephala essential oils are the same as those in Example 1.

[0076] Comparative Example 4

[0077] An essential oil for weight loss. Its preparation method is different from that of Example 1 only in that: take 36 ml of Fructus Aurantii Immaturus essential oil, 36 ml of Atractylodes Macrocephala essential oil, and 18 ml of Magnolia Officinalis essential oil, mix them evenly; then add 18 ml of carrier essential oil (the volume ratio of Amomum Villosum essential oil, Perilla Frutescens essential oil, Cinnamomum Cassia essential oil, Citrus Reticulata Blanco essential oil, and Fructus Aurantii essential oil is 1:2:2:0.5:0.5), and after mixing evenly, divide and package to obtain it.

[0078] The preparation method of Magnolia Officinalis essential oil includes the following steps:

[0079] S1. Pretreatment: Dry and crush Magnolia Officinalis, and pass through a 100-mesh sieve;

[0080] S2. Soaking: Soak the crushed Magnolia Officinalis in double-distilled water for 1 h in step S1, with a material-liquid ratio of 1 g:10 ml;

[0081] S3. Steam distillation: Distill the mixture obtained in step S2, with the distillation temperature set at 100 °C, the pressure at 300 mbar, and the time at 8 h;

[0082] S4. Dehydration: Dehydrate the distillate obtained in step S3 with anhydrous sodium sulfate to obtain Magnolia Officinalis essential oil;

[0083] The preparation methods of Fructus Aurantii Immaturus, Atractylodes Macrocephala, Amomum Villosum, Perilla Frutescens, Cinnamomum Cassia, Citrus Reticulata Blanco, and Fructus Aurantii essential oils are the same as those in Example 1.

[0084] Comparative Example 5

[0085] An essential oil for weight loss, its preparation method is only different from that of Example 1 in that: 36 ml of carrier essential oil (the volume ratio of amomum villosum essential oil, perilla essential oil, cinnamon essential oil, citrus reticulata blanco essential oil, and aurantium fructus essential oil is 1:2:2:0.5:0.5) is taken, and after mixing evenly, it is sub-packed to obtain. The preparation methods of amomum villosum, perilla, cinnamon, citrus reticulata blanco, and aurantium fructus essential oils are the same as those in Example 1.

[0086] Study on the external use effect of the essential oil in Effect Example 1

[0087] 1. Study on the weight loss effect of rats

[0088] (1) Establishment of the rat obesity model

[0089] Adult healthy male SD rats (purchased from the Animal Experiment Research Center of Jiangxi University of Traditional Chinese Medicine), with similar body weight and body fat, were adaptively fed for 1 week and randomly divided into a model group, a normal group, Example 1-4 groups, and Comparative Example 1-5 groups, with 8 rats in each group. The model group was fed a high-fat diet, and the normal group, Example 1-4 groups, and Comparative Example 1-5 groups were fed a normal diet. The feed amount for each rat was the same. After feeding for 30 days, the breeding temperature was (24±2)°C, the relative humidity was 50%-70%, and the light was controlled at 12 h light / 12 h dark. The body weight of each rat was recorded twice a week. When the body weight of the rats in the model group differed by 20% compared with that of the rats in the normal group, it indicated that the modeling was successful.

[0090] (2) Grouping and administration

[0091] After the modeling was successful, the rats in Example 1-4 groups and Comparative Example 1-5 groups were respectively smeared with the essential oils obtained in Example 1-4 and Comparative Example 1-5 once a day. The essential oil was diluted to 5% with jojoba oil, and the smearing site was the Shenque acupoint, with a smearing dose of 1 ml / d, and the administration was continued for 35 days.

[0092] During the administration period, the body weight change of the rats was recorded daily, and the change in the salivary flow increase rate of the rats was measured once a week. The weight loss effect of the essential oil on the rats was evaluated using the body weight; the dehumidifying effect of the essential oil on the rats was evaluated by measuring the salivary flow increase rate of the rats using the cotton ball method. Each dry cotton ball was placed under the tongue of the rat for 30 minutes, and the salivary secretion volume was calculated by weighing the weight difference of the dry cotton balls before and after, and the salivary flow increase rate after 35 days of administration was calculated compared with the initial salivary flow volume. The specific results are shown in Table 1.

[0093] Table 1 Influence of essential oils in each group on the weight loss effect of rats

[0094]

[0095]

[0096] Note: Compared with the normal group, ** indicates P<0.01; compared with the model group,# Indicates P < 0.05, ## Indicates P < 0.01, ### Indicates P < 0.001.

[0097] According to the data in the above table, it can be seen that the essential oil of the embodiment of the present invention can significantly reduce the body weight of rats, indicating that the Fructus Aurantii Immaturus essential oil and Atractylodes macrocephala essential oil in the embodiment of the present invention adopt the specific dosages of the present invention, and the carrier essential oil and the sum of the Fructus Aurantii Immaturus essential oil and Atractylodes macrocephala essential oil adopt a specific volume ratio, which can exert excellent weight loss effects. According to the salivation rate data, it can be seen that the embodiment of the present invention can significantly reduce the salivation volume of rats and has excellent dehumidification effects.

[0098] 2. Study on the skin irritation of essential oil to rats

[0099] Adult healthy male SD rats (purchased from the Animal Experiment Research Center of Jiangxi University of Traditional Chinese Medicine), with similar body weight and body fat, were adaptively fed for 1 week and randomly divided into a control group, Example 1-4 groups, and Comparative Example 1-5 groups, with 8 rats in each group. The room temperature was (24 ± 2) °C and the humidity was 50%-70%. During the experiment, the animals were fed separately in cages, with a regular diet and free access to clean tap water. The hair on the abdomen of each rat was removed, and the area was 2.5 cm × 2.5 cm.

[0100] Among them, in the Example 1-4 groups and the Comparative Example 1-5 groups, the essential oils corresponding to Example 1-4 and Comparative Example 1-5 were respectively applied to the depilated parts of the rats. The essential oil was diluted to 5% with jojoba oil, and the application site was the Shenque acupoint. The application dose was 1 ml / d, and the administration was continued for 7 days. The conditions such as erythema and edema at the administration site were observed and recorded every day.

[0101] Criteria for irritation scoring: No redness, ecchymosis, rash, pigmentation, or skin roughness is recorded as 10 points; no redness, ecchymosis, rash, or pigmentation, but slight skin roughness is recorded as 9 points; no redness, ecchymosis, rash, or pigmentation, but obvious skin roughness is recorded as 8 points; no redness, ecchymosis, rash, or pigmentation, but severe skin roughness is recorded as 7 points; no ecchymosis, rash, or pigmentation, but slight redness and obvious skin roughness are recorded as 6 points; no ecchymosis, rash, or pigmentation, but obvious redness and obvious skin roughness are recorded as 5 points; no ecchymosis, rash, or pigmentation, but severe redness and severe skin roughness are recorded as 4 points; slight ecchymosis, slight rash, slight pigmentation, severe redness, and severe skin roughness are recorded as 3 points; obvious ecchymosis or obvious rash or obvious pigmentation, severe redness, and severe skin roughness are recorded as 2 points; obvious ecchymosis, obvious rash, obvious pigmentation, severe redness, and severe skin roughness are recorded as 1 point; severe ecchymosis, severe rash, obvious pigmentation, severe redness, and severe skin roughness are recorded as 0 points.

[0102] The specific results are shown in Table 2.

[0103] Table 2 Effects of essential oils of each group on skin irritation of rats

[0104]

[0105] According to the results in Table 2, it can be seen that the essential oil obtained in the embodiment of the present invention has no irritation to the skin of rats and has high safety.

[0106] Study on the oral administration effect of essential oil in Effect Example 2

[0107] 1. Study on the weight loss effect of rats

[0108] The method for establishing the obese rat model is the same as that in Effect Example 1. After successful modeling, the essential oil prepared in the Example 1 group was diluted to 3%, and gavaged once a day, with the administration dose of 200 μL / kg, and continuously administered for 35 days. During the administration period, the body weight change of the rats was recorded daily, and the change of the salivary flow increase rate of the rats was measured weekly. The weight loss effect of the essential oil on the rats was evaluated by body weight; the dehumidifying effect of the essential oil on the rats was evaluated by measuring the salivary flow increase rate of the rats using the cotton ball method. Each dry cotton ball was placed under the tongue of the rats for 30 minutes, and the salivary secretion amount was calculated by weighing the weight difference of the dry cotton balls before and after, and the salivary flow increase rate after 35 days of administration was calculated compared with the initial salivary flow amount. The specific results are shown in Table 3. After the last administration, the rats were fasted overnight, the liver of the rats was dissected, and the liver index of the rats was detected. The liver index (%) = liver mass / body weight after 35 days of administration * 100. The specific results are shown in Table 4.

[0109] Table 3 Effects of Oral Essential Oil on Weight Loss in Rats

[0110]

[0111] Note: Compared with the normal group, ** indicates P < 0.01; compared with the model group, ### indicates P < 0.001.

[0112] According to the results of the above table, the essential oil of the embodiment of the present invention can be taken orally, significantly reducing the body weight and salivation volume of rats, and having excellent weight loss effects.

[0113] 2. Effects of Essential Oil on Rat Liver

[0114] Table 4 Effects on Rat Liver Index after Administration

[0115] Group Liver index / % Normal group 2.97±0.22 Model group 3.08±0.31 Example 1 3.05±0.24

[0116] The above results show that after continuous intragastric administration of the essential oil prepared in the embodiment of the present invention for 35 days, there is no obvious effect on the liver index of rats, proving that the essential oil prepared from the medicated and edible Chinese herbs of the present invention has no toxic effect on rats and is safe and reliable.

[0117] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than limiting the protection scope of the present invention. Any simple modification or equivalent replacement of the technical solution of the present invention by those of ordinary skill in the art shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. An essential oil for weight loss, characterized in that: It is composed of the following components in parts by volume: 9 - 20 parts of Fructus Aurantii Immaturus essential oil, 12 - 27 parts of Atractylodes Macrocephala essential oil, and carrier essential oil; the volume ratio of the carrier essential oil to the sum of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil is 3:11 - 17; The carrier essential oil is Amomum villosum essential oil, Perilla frutescens essential oil, Cinnamomum cassia essential oil, Citrus reticulata Blanco essential oil, and Poncirus trifoliata essential oil; or Citrus reticulata Blanco var. austera essential oil, Pogostemon cablin essential oil, Myristica fragrans essential oil, Aucklandia lappa essential oil, and Citrus medica L. var. sarcodactylis essential oil; or Citrus aurantium L. var. amara Engl. essential oil, Eugenia caryophyllata essential oil, Citrus reticulata Blanco essential oil, Foeniculum vulgare Mill. essential oil, and Zanthoxylum bungeanum Maxim. essential oil; or Atractylodes lancea essential oil, Perilla frutescens essential oil, Artemisia capillaris Thunb. essential oil, Boswellia carterii Birdw. essential oil, and Zingiber officinale Roscoe essential oil; The preparation method of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil includes the following steps: S1. Pretreatment: Respectively dry and crush Fructus Aurantii Immaturus and Atractylodes Macrocephala, and sieve through a 80 - 100 mesh sieve; S2. Soaking: Respectively soak the crushed Fructus Aurantii Immaturus and Atractylodes Macrocephala in step S1 with double - distilled water to obtain a Fructus Aurantii Immaturus mixture and an Atractylodes Macrocephala mixture; S3. Distillation: Respectively carry out distillation treatment on the Fructus Aurantii Immaturus mixture and the Atractylodes Macrocephala mixture in step S2; S4. Drying: Use anhydrous sodium sulfate to respectively carry out drying treatment on the distilled Fructus Aurantii Immaturus fraction and Atractylodes Macrocephala fraction to obtain Fructus Aurantii Immaturus essential oil and Atractylodes Macrocephala essential oil.

2. The essential oil according to claim 1, wherein: The carrier essential oil is Amomum villosum essential oil, Perilla frutescens essential oil, Cinnamomum cassia essential oil, Citrus reticulata Blanco essential oil, and Poncirus trifoliata essential oil.

3. The preparation method of the essential oil according to any one of claims 1-2, characterized in that: It includes the following steps: S1. Mix the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil evenly according to the required ratio; S2. Add the formulated amount of carrier essential oil to the mixed essential oil obtained in step S1, mix evenly and then sub - package to obtain.

4. The preparation method according to claim 3, characterized in that: The preparation method of the Fructus Aurantii Immaturus essential oil and the Atractylodes Macrocephala essential oil includes the following steps: S1. Pretreatment: Respectively dry and crush Fructus Aurantii Immaturus and Atractylodes Macrocephala, and sieve through a 80 - 100 mesh sieve; S2. Soaking: Respectively soak the crushed Fructus Aurantii Immaturus and Atractylodes Macrocephala in step S1 with double - distilled water to obtain a Fructus Aurantii Immaturus mixture and an Atractylodes Macrocephala mixture; S3. Distillation: Respectively carry out distillation treatment on the Fructus Aurantii Immaturus mixture and the Atractylodes Macrocephala mixture in step S2; S4. Drying: Use anhydrous sodium sulfate to respectively carry out drying treatment on the distilled Fructus Aurantii Immaturus fraction and Atractylodes Macrocephala fraction to obtain Fructus Aurantii Immaturus essential oil and Atractylodes Macrocephala essential oil.

5. The preparation method according to claim 4, characterized in that: In the double - distilled water soaking in step S2, the material - liquid ratio of Fructus Aurantii Immaturus / Atractylodes Macrocephala to double - distilled water is 1 g:5 - 7 ml; the soaking conditions in step S2 are: temperature 15 - 30 °C, time 40 - 80 min; the distillation conditions in step S3 are: temperature 80 - 90 °C, pressure 300 - 350 mbar, time 5 - 6 h.

6. Use of the essential oil according to any one of claims 1 - 2 or the essential oil prepared by the preparation method according to any one of claims 3 - 5 in the preparation of a preparation for treating human obesity.

7. The application according to claim 6, wherein: The preparation includes an oral preparation and a non - oral administration preparation; the oral preparation includes microcapsules, dripping pills, and oral liquids; the non - oral administration preparation includes inhalants and transdermal medicaments.

8. An oral preparation, characterized in that: Prepared from the essential oil according to any one of claims 1 - 2.

9. A non-oral preparation, characterized in that: Prepared from the essential oil according to any one of claims 1 - 2.

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