Micro-volume automated pipetting workstation

Abstract

The present invention relates to the technical field of pipetting workstations, and discloses a micro-automated pipetting workstation, comprising a pipetting assembly, a workbench, and a tray member, wherein the workbench is used to carry the tray member or drive the tray member to move, the tray member is used to place a feed tray, a mother tray, and a daughter tray, the feed tray is used to place a pipette, and the mother tray and the daughter tray are respectively used to receive liquid; the pipetting assembly comprises a transfer structure, a tube plate, and a lifting structure, the transfer structure is used to drive the tube plate to move, the tube plate is used to take a plurality of pipettes, the pipette comprises a tube body and a piston needle, the lifting structure is used to drive the piston needle to move relative to the tube body, the piston needle is used to absorb or distribute liquid, the lower end of the piston needle forms a lower needle portion, and when distributing liquid, the lower needle portion moves to the outside of the tube body for arrangement. The volume of the pipetting workstation is greatly reduced, and at the same time, the absorption and distribution of trace liquids are achieved, meeting the needs of micro-pipetting, and reducing liquid residue, thereby greatly ensuring the accuracy of the data used in micro-experiments.

Description

Technical Field

[0001] The present invention relates to the technical field of pipetting workstations, and more specifically, to micro-automated pipetting workstations. Background Art

[0002] A pipetting workstation is an analytical instrument used in the field of biology, mainly used to transfer liquid samples. With the development of society, pipetting workstations tend to be automated, thereby reducing manpower, material and time costs, while also reducing pipetting errors.

[0003] For example, the prior patent with publication number CN211896907U discloses a multifunctional liquid workstation, including a support frame and a base plate arranged in the support frame, on which a gun tip storage box area, an oscillation module, a consumable well plate placement area, a centrifuge, a reagent storage area, a temperature control module and a nucleic acid extraction module are provided; the top of the support frame is provided with a pipetting robot arm for pipetting and a plate moving robot arm for transferring well plates, which systematically integrates functions such as pipetting, plate moving, centrifugation, oscillation, heating, cooling and magnetic rod nucleic acid extraction. The functional modules of the liquid workstation can be combined and disassembled, and can be applied to different scenarios and costs. It not only realizes the high-throughput micro-nucleic acid extraction function, but also can be used as a universal liquid workstation for pipetting and other biological experiments. Automation operation.

[0004] In the existing technology, the pipetting workstation is large in size and occupies a large space, which has limitations in usage scenarios. At the same time, the existing pipetting workstation cannot meet the needs of micro-amount suction and distribution of liquids, and cannot meet the needs of micro-pipetting. Moreover, the existing technology uses a pipetting head to perform pipetting, which easily leads to liquid residue and affects the accuracy of the experiment. Summary of the Invention

[0005] The purpose of the present invention is to provide a micro-automatic pipetting workstation, aiming to solve the problem of poor experimental accuracy of the pipetting workstation in the prior art.

[0006] The present invention is achieved as follows: a micro-automated pipetting workstation includes a pipetting assembly, a workbench and a tray component, the workbench is used to carry the tray component or drive the tray component to be movable, the tray component is used to place a feed tray, a mother tray and a child tray, the feed tray is used to place a pipette, and the mother tray and the child tray are used to receive liquid respectively; the pipetting assembly includes a transfer structure, a tube disc plate and a lifting structure, the tube disc plate and the transfer structure are assembled, the transfer structure is used to drive the tube disc plate to be movable, the tube disc plate is used to take multiple pipettes in the same batch, the pipette includes a tube body and a piston needle, the piston needle is embedded in the tube body, the lifting structure is arranged above each piston needle, the lifting structure is used to synchronously dock each piston needle, and the lifting structure is used to drive the piston needle to be movable relative to the tube body, the piston needle is used to absorb or dispense liquid when it moves, the lower end of the piston needle forms a lower needle part, and when dispensing liquid, the lower needle part moves to the outside of the tube body.

[0007] Furthermore, the lifting structure includes a lifting plate and multiple fixed pipe heads, each of which is respectively installed on the tube disc plate. The lifting plate is arranged to rise or fall under the driving force, and the lifting plate is connected to the tube disc plate. The fixed pipe head is used to be assembled with the piston needle, and the fixed pipe head is used to drive the piston needle to move relative to the tube body.

[0008] Furthermore, the fixed tube head includes a fixed tube shell and an opener and closer, the opener and closer is arranged inside the fixed tube shell, and the opener and closer is arranged in an open or clamping manner; the upper end portion of the piston needle forms an upper needle portion, and the upper needle portion has a needle groove, the needle groove is arranged in an annular shape, and the needle groove is arranged toward a recessed position, and the opener and closer is embedded in the needle groove in a clamping manner.

[0009] Furthermore, the lifting structure includes a tube removal plate, which is arranged horizontally and below the lifting plate. The tube removal plate is arranged to rise or fall under the driving force; each of the fixed pipe heads synchronously passes through the tube removal plate, and the tube removal plate is arranged above the pipe body. The tube removal plate is used to synchronously drive each of the pipe bodies to separate from the fixed pipe heads.

[0010] Furthermore, the transfer structure includes a transfer plate and a transfer motor, the transfer plate and the tube plate are arranged in an upper and lower overlapping and docking manner, the transfer motor and the transfer plate are connected, the material tray, the mother tray and the sub-tray are arranged in an interval arrangement along the Y direction, and the transfer motor is used to drive the transfer plate to move along the Y direction.

[0011] Furthermore, the material tray is provided with a plurality of tube modules, the tube module includes four pipettes, the tube disk plate is used to take four pipettes in the same batch, and the four pipettes are arranged in a straight line at intervals; or, the tube module includes eight pipettes, the tube disk plate is used to take eight pipettes in the same batch, and the eight pipettes are arranged in a straight line at intervals; or, the tube module includes sixteen pipettes, the tube disk plate is used to take sixteen pipettes in the same batch, and the sixteen pipettes are arranged in an array at intervals; the tube module includes forty-eight pipettes, the tube disk plate is used to take forty-eight pipettes in the same batch, and the forty-eight pipettes are arranged in an array at intervals; the tube module includes ninety-six pipettes, the tube disk plate is used to take ninety-six pipettes in the same batch, and the ninety-six pipettes are arranged in an array at intervals.

[0012] Furthermore, the workbench includes two table plates, a disk drive and two driving belts, the two table plates are arranged in a corresponding manner at intervals, the table plates have guide rails, the two guide rails are arranged in alignment at the same level and are used to carry the pallet parts; the pallet parts include a main pallet and two disk moving blocks, the main pallet is synchronously arranged with the two disk moving blocks in an upper and lower docking arrangement, and the main pallet is used for placing the material tray, the mother tray and the sub-tray; the two driving belts are arranged in a one-to-one correspondence with the two disk moving blocks and are connected, the disk drive is used to synchronously drive the two driving belts to be in a conveying state, and the driving belt is used to drive the disk moving blocks to be in a moving arrangement; the main pallet is provided with a plurality of stabilizing columns, the stabilizing columns are arranged longitudinally, and the stabilizing column sleeves are provided with stabilizing rings, the stabilizing rings are elastically arranged, and the stabilizing rings are arranged in conflict with the table plates.

[0013] Furthermore, the main tray includes two sub-tray parts, and the two sub-tray parts are arranged in sequence along the conveying direction of the workbench. The two sub-tray parts are respectively used to place the material tray, the mother tray and the sub-tray. A pipetting area is formed below the pipetting assembly, and the workbench is used to switch the two sub-tray parts to be in the pipetting area respectively.

[0014] Furthermore, the tray portion includes a material placement portion, a mother placement portion and a child placement portion, and the material placement portion, the mother placement portion and the child placement portion are arranged at intervals along the Y direction; the material placement portion includes four material placement blocks, and the four material placement blocks are arranged at four corners, and the material placement blocks have material placement surfaces, and the material placement surfaces are arranged in an arc shape, and the material placement surfaces are arranged in a low inner and high outer shape, and the four material placement surfaces are arranged flush along the horizontal plane direction, and the four material placement surfaces are used to synchronously carry the material tray; the mother placement portion includes four mother placement blocks, and the four mother placement blocks are arranged at four corners Arrangement, the mother placing block has a mother placing surface, the mother placing surface is arranged in an arc surface shape, and the mother placing surface is arranged with an inner low and an outer high shape, the four mother placing surfaces are arranged flush along the horizontal plane direction, and the four mother placing surfaces are used to synchronously carry the mother disk; the child placing part includes four child placing blocks, the four child placing blocks are arranged in four corners, the child placing blocks have child placing surfaces, the child placing surfaces are arranged in an arc surface shape, and the child placing surfaces are arranged with an inner low and an outer high shape, the four child placing surfaces are arranged flush along the horizontal plane direction, and the four child placing surfaces synchronously carry the child disk.

[0015] Furthermore, the micro-automated pipetting workstation includes a material collection box, which is arranged below the tray member, and the material collection box is arranged with a top opening. The tray member has a material removal port, and the material removal port and the material collection box are arranged in upper and lower correspondence. The removal port is used for the pipette to pass through and fall into the material collection box.

[0016] The present invention also provides a micro-automatic pipetting workstation, which is convenient for use in the field application, and a micro-automatic pipetting workstation, which is convenient for use in the field application, and a micro-automatic pipetting workstation, which is convenient for use in the field application, and a micro-automatic pipetting workstation, which is convenient for use in the field application, and a micro-automatic pipetting workstation, which is convenient for use in the field application, BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional schematic diagram of the micro-automatic pipetting workstation provided by the present invention;

[0018] Figure 2 It is a three-dimensional schematic diagram of the transfer structure of the micro-automatic pipetting workstation provided by the present invention;

[0019] Figure 3 It is a cross-sectional schematic diagram of the pipette of the micro-automatic pipetting workstation provided by the present invention in a liquid aspirating state;

[0020] Figure 4 It is a cross-sectional schematic diagram of a pipette of the micro-automatic pipetting workstation provided by the present invention in an empty state;

[0021] Figure 5 It is an enlarged schematic diagram of part A of the micro-automatic pipetting workstation provided by the present invention. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0023] The implementation of the present invention is described in detail below with reference to specific embodiments.

[0024] The same or similar numbers in the drawings of this embodiment correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", etc. indicate the orientation or position relationship, they are based on the orientation or position relationship shown in the drawings. This is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, the terms describing the position relationship in the drawings are only used for illustrative purposes and cannot be understood as limiting this patent. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0025] Reference Figure 1-5 The figure shows a preferred embodiment of the present invention.

[0026] The micro-automatic pipetting workstation includes a pipetting assembly 1, a workbench 2 and a tray 3. The workbench 2 is used to carry the tray 3 or drive the tray 3 to be mobile. The tray 3 is used to place the feed tray, the mother tray and the child tray. The feed tray is used to place the pipette 4. The mother tray and the child tray are used to receive the liquid 6 respectively. The pipetting assembly 1 includes a transfer structure, a tube plate 12 and a lifting structure 11. The tube plate 12 is assembled with the transfer structure. The transfer structure is used to drive the tube plate 12 to be mobile. The tube plate 12 is used to Take multiple pipettes 4 in batches. The pipette 4 includes a tube body 41 and a piston needle 42. The piston needle 42 is embedded in the tube body 41. The lifting structure 11 is arranged above each piston needle 42. The lifting structure 11 is used to synchronously dock each piston needle 42, and the lifting structure 11 is used to drive the piston needle 42 to move relative to the tube body 41. When the piston needle 42 moves, it is used to absorb or distribute liquid 6. The lower end of the piston needle 42 forms a lower needle part 43. When distributing liquid 6, the lower needle part 43 moves to the outside of the tube body 41.

[0027] The above-mentioned micro-automatic pipetting workstation, when performing pipetting operations, the transfer structure drives the tube disc plate 12 to move to the top of the material disc, and multiple pipettes 4 are taken out in the same batch through the tube disc plate 12. The transfer structure then moves to the top of the mother disc, and then cooperates with the lifting structure 11 to drive the piston needle 42 to move upward relative to the tube body 41, thereby sucking the liquid 6 on the mother disc, and then transferring it to the top of the daughter disc, and then driving the piston needle 42 to move downward relative to the tube body 41 through the lifting structure 11 to discharge the liquid 6 to the daughter disc, so that the liquid 6 on the mother disc is mixed with the liquid 6 on the daughter disc. The liquid 6 in the sub-disc is mixed to complete automatic pipetting; in this way, the volume of the pipetting workstation is greatly reduced, which is convenient for the layout and use of the pipetting workstation. At the same time, the liquid 6 is absorbed and distributed by the micropipette 4, so as to realize the absorption and distribution of trace liquid 6 and meet the needs of micro-pipetting. In addition, during the discharge process of the pipette 4, under the action of the lower needle part 43, the piston needle 42 maximizes the scraping of the liquid 6 remaining in the tube body 41, thereby reducing the residue of the liquid 6. In the micro-experiment, the accuracy of the data used is greatly guaranteed.

[0028] At the same time, under the action of the lower needle portion 43, the micropipette 4 avoids the generation of bubbles when sucking the liquid 6, thereby improving the accuracy of data in micro experiments.

[0029] The lower part of the tube body 41 forms a bottom tube portion, which has a bottom tube wall, and the bottom tube wall is arranged in a surrounding manner; the outer surface of the lower needle portion 43 is arranged flatly and in contact with the bottom tube wall; the scraping effect of the lower needle portion 43 on the tube body 41 is ensured to reduce the residual amount of the liquid 6.

[0030] The lower portion of the lower needle portion 43 is in a frustum shape to avoid direct impact with the tube body 41 and improve the coordination effect between the tube body 41 and the piston needle 42 .

[0031] The tube body 41 includes an upper tube section and a lower tube section, which are butt-jointed and integrally formed. The diameter of the upper tube section is larger than that of the lower tube section. The piston needle 42 has a lower stop block, and the diameter of the lower stop block is larger than that of the lower tube section. When the lower stop block is blocked by the lower tube section, the piston needle 42 completes the downward discharge of the liquid 6 to the sub-disc, and at this time the lower needle part 43 is arranged outside the lower tube section.

[0032] An upper stop piece is provided inside the lower tube section, and the piston needle 42 is arranged through the upper stop piece. The middle part of the upper stop piece forms a piece opening, and the diameter of the lower needle part 43 is larger than the piece opening; in this way, the piston needle 42 is restricted from separating from the tube body 41, ensuring the assembly of the tube body 41 and the piston needle 42, which facilitates the taking and unloading of materials by the pipette 4.

[0033] The lifting structure 11 includes a lifting plate and multiple fixed pipe heads 13, each of which is respectively installed on the tube disc plate 12 to realize the installation of each fixed pipe head 13. The lifting plate is arranged to rise or fall under the driving force, and the lifting plate and the tube disc plate 12 are connected. The lifting plate drives the tube disc plate 12 to rise or fall. The fixed pipe head 13 is used to be assembled with the piston needle 42, and the fixed pipe head 13 is used to drive the piston needle 42 to move relative to the tube body 41; when the lifting plate is driven to rise or fall, it drives the tube disc plate 12 to rise or fall, and the movement of the tube disc plate 12 will synchronously drive each fixed pipe head 13 to rise or fall, so that each fixed pipe head 13 is synchronously assembled with each piston needle 42, so that the automatic picking of each pipette 4 is realized.

[0034] The fixed pipe head 13 includes a fixed pipe shell and an opener and closer. The opener and closer is arranged inside the fixed pipe shell. The opener and closer is arranged in an open or clamping manner. The upper end of the piston needle 42 forms an upper needle portion. The upper needle portion has a needle groove. The needle groove is arranged in an annular shape and the needle groove is arranged toward the recess. The opener and closer is embedded in the needle groove in a clamping manner.

[0035] In this way, after the piston needle 42 and the fixing pipe head 13 are assembled, the upper needle portion is clamped by the opener and closeer to enhance the assembly stability of the piston needle 42 and ensure the subsequent driving of the piston needle 42.

[0036] The opener can clamp the upper needle portion by utilizing centrifugal force through rotation.

[0037] Alternatively, the opener can be driven by a pneumatic cylinder to switch between clamping and opening.

[0038] The lifting structure 11 includes a tube removal plate 14, which is arranged horizontally and below the lifting plate. The tube removal plate 14 is arranged to rise or fall under the driving force; each fixed pipe head 13 is arranged to pass through the tube removal plate 14 synchronously, and the tube removal plate 14 is arranged above the pipe body 41. The tube removal plate 14 is used to synchronously drive each pipe body 41 to separate from the fixed pipe head 13.

[0039] In this way, after the pipette 4 discharges liquid into the sub-disc, the transfer structure drives the tube disc plate 12 to move to the specified position. At this time, the tube removal plate 14 receives the driving force and moves downward, prompting the tube removal plate 14 to synchronously resist the application of each tube body 41, driving each pipette 4 to detach from the fixed tube head 13, realizing automatic unloading and circulation. At the same time, each pipette 4 is disposable, avoiding cross-contamination of samples, and avoiding time-consuming, expensive and unreliable solvent cleaning links, and completely eliminating many reliability problems caused by blockage, bending or leakage.

[0040] The transfer structure includes a transfer plate and a transfer motor. The transfer plate and the tube disc plate 12 are arranged in an upper and lower overlapping and docking manner. The transfer motor and the transfer plate are connected. The material disc, mother disc and child disc are arranged in an interval arrangement along the Y direction. The transfer motor is used to drive the transfer plate to move along the Y direction to realize the driving of the tube disc plate 12.

[0041] The pipetting assembly 1 can be positioned accurately to 0.1 mm through the X, Y, and Z axes, so that the pipette 4 can accurately enter the mother plate to absorb the liquid 6. At the same time, it ensures that the liquid 6 is transferred to the sub-plate, facilitating the perfect overlap or mixing of the liquid 6 in the sub-plate.

[0042] Pipette 4 can handle different solvents, solutions and suspensions with a wide range of viscosities; therefore, there is no need to change the specifications of pipette 4 for different applications, and the volume increment can be programmed to 1nL. Pipette 4 also has the functions of single aspiration followed by multiple additions, multiple aspirations followed by single addition, and sample mixing.

[0043] The material tray is provided with a plurality of tube modules, each of which includes four pipettes 4. The tube tray plate 12 is used to take four pipettes 4 in the same batch, and the four pipettes 4 are arranged correspondingly along a straight line.

[0044] Among the four pipettes 4 , the interval between adjacent pipettes 4 is 4.5 mm, or the interval between adjacent pipettes 4 is 9 mm.

[0045] Alternatively, the tube module includes eight pipettes 4 , and the tube plate 12 is used to take eight pipettes 4 in the same batch, and the eight pipettes 4 are arranged in a linear manner at intervals.

[0046] Among the eight pipettes 4 , the interval between adjacent pipettes 4 is 4.5 mm, or the interval between adjacent pipettes 4 is 9 mm.

[0047] Alternatively, the tube module includes sixteen pipettes 4, and the tube plate 12 is used to take sixteen pipettes 4 in the same batch, and the sixteen pipettes 4 are arranged in an array at intervals.

[0048] Among the sixteen pipettes 4 , the interval between adjacent pipettes 4 is 4.5 mm.

[0049] The tube module includes forty-eight pipettes 4. The tube plate 12 is used to take the forty-eight pipettes 4 in the same batch. The forty-eight pipettes 4 are arranged in an array at intervals.

[0050] Among the forty-eight pipettes 4 , the interval between adjacent pipettes 4 is 4.5 mm.

[0051] The tube module includes ninety-six pipettes 4. The tube plate 12 is used to take ninety-six pipettes 4 in the same batch. The ninety-six pipettes 4 are arranged in an array at intervals.

[0052] Among the ninety-six pipettes 4 , the interval between adjacent pipettes 4 is 4.5 mm.

[0053] The workbench 2 includes two platform plates, a disk drive and two driving belts. The two platform plates are arranged in a corresponding manner at intervals. The platform plates have guide rails. The two guide rails are aligned at the same level and are used to carry the pallet component 3; the pallet component 3 includes a main pallet and two disk transfer blocks. The main pallet is synchronously connected to the two disk transfer blocks in an upper and lower docking arrangement. The main pallet is used for placing the feed tray, mother tray and sub-tray; the two driving belts are arranged in a one-to-one correspondence with the two disk transfer blocks and are connected. The disk drive is used to synchronously drive the two driving belts to a conveying state, and the driving belts are used to drive the disk transfer blocks to a moving arrangement; in this way, the pallet component 3 is driven, thereby realizing the change of the position of the pallet component 3, which is convenient for replenishing the pipette 4 and the liquid 6.

[0054] The main pallet is provided with multiple stabilizing columns, which are arranged longitudinally, and the stabilizing column sleeves are provided with stabilizing rings, which are elastically arranged and arranged in conflict with the platform plate; under the action of the stabilizing columns, the movement deviation of the pallet component 3 is limited to ensure the smooth movement of the pallet component 3.

[0055] The main tray includes two sub-tray parts, which are arranged in sequence along the conveying direction of the workbench 2. The two sub-tray parts are used to place the feed tray, the mother tray and the child tray respectively. A pipetting area is formed under the pipetting assembly 1, and the workbench 2 is used to switch the two sub-tray parts to the pipetting area respectively; in this way, the switching of the two sub-tray parts is realized, and feeding and pipetting are carried out at the same time, thereby improving the overall experimental efficiency.

[0056] The tray portion includes a material placement portion, a mother placement portion and a child placement portion, which are arranged at intervals along the Y direction; the material placement portion includes four material placement blocks, which are arranged at four corners, and the material placement blocks have material placement surfaces, which are arranged in an arc shape, and the material placement surfaces are arranged with the inside lower and the outside higher. The four material placement surfaces are arranged flush along the horizontal plane, and the four material placement surfaces are used to synchronously carry the material tray; in this way, when the material tray is placed, it can be more centered and stable, which is convenient for the subsequent batch taking of each pipette 4.

[0057] The master placement portion includes four master placement blocks, which are arranged at four corners. The master placement blocks have master placement surfaces, which are arranged in an arc shape, and the master placement surfaces are arranged with the inside low and the outside high. The four master placement surfaces are arranged flush along the horizontal plane, and the four master placement surfaces are used to synchronously support the master disc; in this way, when the master disc is placed, it can be more centered and stable, which is convenient for the subsequent pipette 4 to align with the various liquids 6 on the master disc, and for the pipette 4 to absorb the liquid 6.

[0058] The sub-placement portion includes four sub-placement blocks, which are arranged at four corners. The sub-placement blocks have sub-placement surfaces, which are arranged in an arc shape, and the sub-placement surfaces are arranged with the inside low and the outside high. The four sub-placement surfaces are arranged flush along the horizontal plane, and the four sub-placement surfaces synchronously support the sub-plate; in this way, when the sub-plate is placed, it can be more centered and stable, which is convenient for the subsequent alignment of the pipette 4 with the dripping position of the sub-plate, and for the pipette 4 to discharge the liquid 6 into the sub-plate.

[0059] The micro-automatic pipetting workstation includes a collection box 5, which is arranged below the tray part 3. The collection box 5 is arranged with an open top. The tray part 3 has a discharge port, which is arranged corresponding to the collection box 5 in the upper and lower parts. The discharge port is used for the pipette 4 to pass through and fall into the collection box 5; under the action of the collection box 5, it is convenient to collect the pipette 4 after the pipetting operation.

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. Micro-automatic pipetting workstation, characterized in that, The device comprises a pipette assembly, a workbench and a tray component, wherein the workbench is used to carry the tray component or drive the tray component to be movable, the tray component is used to place a feed tray, a mother tray and a child tray, the feed tray is used to place a pipette, and the mother tray and the child tray are used to receive liquid respectively; the pipette assembly comprises a transfer structure, a tube disc plate and a lifting structure, the tube disc plate and the transfer structure are assembled, the transfer structure is used to drive the tube disc plate to be movable, the tube disc plate is used to take a plurality of the pipettes in the same batch, the pipette comprises a tube body and a piston needle, the piston needle is embedded in the tube body, the lifting structure is arranged above each of the piston needles, the lifting structure is used to synchronously dock each of the piston needles, and the lifting structure is used to drive the piston needle to be movable relative to the tube body, the piston needle is used to absorb or dispense liquid when it moves, the lower end of the piston needle forms a lower needle part, and when dispensing liquid, the lower needle part moves to the outside of the tube body; The lower part of the tube body forms a bottom tube part, and the bottom tube part has a bottom tube wall, and the bottom tube wall is arranged in a surrounding enclosure; the outer surface of the lower needle part is flatly arranged in conflict with the bottom tube wall; the lower part of the lower needle part is frustum-shaped, and the tube body includes an upper tube section and a lower tube section, and the upper tube section and the lower tube section are docked and integrally formed, and the diameter of the upper tube section is larger than the diameter of the lower tube section; the piston needle has a lower stop block, and the diameter of the lower stop block is larger than the diameter of the lower tube section; when the lower stop block is blocked by the lower tube section, the piston needle completes the downward discharge of liquid to the sub-disk, and at this time the lower needle part is arranged outside the lower tube section; an upper stop plate is provided inside the lower tube section, and the piston needle passes through the upper stop plate, and a plate opening is formed in the middle of the upper stop plate, and the diameter of the lower needle part is larger than the plate opening.

2. The micro-automatic pipetting workstation according to claim 1, wherein: The lifting structure includes a lifting plate and multiple fixed pipe heads, each of which is installed on the tube disc plate. The lifting plate is arranged to rise or fall under the driving force, and the lifting plate is connected to the tube disc plate. The fixed pipe head is used to be assembled with the piston needle, and the fixed pipe head is used to drive the piston needle to move relative to the tube body.

3. The micro-automatic liquid transfer workstation according to claim 2, wherein: The fixed pipe head includes a fixed pipe shell and an opener and closer, the opener and closer is arranged inside the fixed pipe shell, and the opener and closer is arranged in an open or clamping manner; the upper end of the piston needle forms an upper needle portion, and the upper needle portion has a needle groove, the needle groove is arranged in an annular shape, and the needle groove is arranged toward the recess, and the opener and closer is embedded in the needle groove in a clamping manner.

4. The micro-automatic pipetting workstation according to claim 3, wherein: The lifting structure includes a tube removal plate, which is arranged horizontally and below the lifting plate. The tube removal plate is arranged to rise or fall under the driving force; each of the fixing pipe heads is synchronously inserted through the tube removal plate, and the tube removal plate is arranged above the pipe body. The tube removal plate is used to synchronously drive each of the pipe bodies to separate from the fixing pipe heads.

5. The micro-automatic pipetting workstation according to any one of claims 1 to 4, characterized in that: The transfer structure includes a transfer plate and a transfer motor. The transfer plate and the tube plate are arranged to be overlapped and docked with each other. The transfer motor is connected to the transfer plate. The material tray, the mother tray and the child tray are arranged at intervals along the Y direction. The transfer motor is used to drive the transfer plate to move along the Y direction.

6. The micro-automatic liquid handling workstation according to any one of claims 1 to 4, wherein: The material tray is provided with a plurality of tube modules, the tube module includes four pipettes, the tube disk plate is used to take four pipettes in the same batch, and the four pipettes are arranged in a straight line at intervals; or, the tube module includes eight pipettes, the tube disk plate is used to take eight pipettes in the same batch, and the eight pipettes are arranged in a straight line at intervals; or, the tube module includes sixteen pipettes, the tube disk plate is used to take sixteen pipettes in the same batch, and the sixteen pipettes are arranged in an array at intervals; the tube module includes forty-eight pipettes, the tube disk plate is used to take forty-eight pipettes in the same batch, and the forty-eight pipettes are arranged in an array at intervals; the tube module includes ninety-six pipettes, the tube disk plate is used to take ninety-six pipettes in the same batch, and the ninety-six pipettes are arranged in an array at intervals.

7. The micro-automatic pipetting workstation according to any one of claims 1 to 4, wherein: The workbench includes two table plates, a disk drive and two driving belts, the two table plates are arranged in a corresponding manner at intervals, the table plates have guide rails, the two guide rails are arranged in alignment at the same level and are used to carry the pallet parts; the pallet parts include a main pallet and two disk moving blocks, the main pallet is synchronously arranged with the two disk moving blocks in an upper and lower docking arrangement, the main pallet is used for placing the material tray, the mother tray and the sub-tray; the two driving belts are in one-to-one correspondence with the two disk moving blocks and are connected, the disk drive is used to synchronously drive the two driving belts to be in a conveying state, and the driving belt is used to drive the disk moving blocks to be in a moving arrangement; the main pallet is provided with a plurality of stabilizing columns, the stabilizing columns are arranged longitudinally, and the stabilizing column sleeves are provided with stabilizing rings, the stabilizing rings are elastically arranged, and the stabilizing rings are arranged in conflict with the table plates.

8. The micro-automatic pipetting workstation according to claim 7, wherein: The main tray includes two sub-tray parts, which are arranged in sequence along the conveying direction of the workbench. The two sub-tray parts are respectively used to place the material tray, the mother tray and the child tray. A pipetting area is formed below the pipetting assembly, and the workbench is used to switch the two sub-tray parts to be in the pipetting area respectively.

9. The micro-automatic pipetting workstation according to claim 8, wherein: The tray portion includes a material placement portion, a mother placement portion and a child placement portion, and the material placement portion, the mother placement portion and the child placement portion are arranged at intervals along the Y direction; the material placement portion includes four material placement blocks, and the four material placement blocks are arranged at four corners. The material placement blocks have material placement surfaces, and the material placement surfaces are arranged in an arc shape, and the material placement surfaces are arranged in a shape of low inside and high outside. The four material placement surfaces are arranged flush along the horizontal plane, and the four material placement surfaces are used to synchronously carry the material tray; the mother placement portion includes four mother placement blocks, and the four mother placement blocks are arranged at four corners The mother placing block has a mother placing surface, the mother placing surface is arranged in an arc shape, and the mother placing surface is arranged with the inner side lower and the outer side higher, the four mother placing surfaces are arranged flush along the horizontal plane, and the four mother placing surfaces are used to synchronously carry the mother disk; the child placing part includes four child placing blocks, the four child placing blocks are arranged at four corners, the child placing blocks have child placing surfaces, the child placing surfaces are arranged in an arc shape, and the child placing surfaces are arranged with the inner side lower and the outer side higher, the four child placing surfaces are arranged flush along the horizontal plane, and the four child placing surfaces synchronously carry the child disk.

10. The micro-automatic pipetting workstation according to any one of claims 1 to 4, characterized in that: The micro-automated pipetting workstation includes a material collection box, which is arranged below the tray member. The material collection box is arranged with a top opening. The tray member has a material removal port, which is arranged in correspondence with the material collection box in the upper and lower directions. The material removal port is used for the pipette to pass through and fall into the material collection box.

Images