Preparation method of DNA-osmium-silver composite nanoparticles

Abstract

The present invention relates to the field of material preparation, and particularly to a method for preparing DNA-osmium-silver composite nanoparticles. It mainly includes the preparation of osmium nanoparticles, the obtaining of mixture 1, and the generation of DNA-osmium-silver composite nanoparticles. The DNA-osmium-silver composite nanoparticles have a high yield, strong peroxidase-like activity, and are cheaper than the individual osmium nanomaterials.

Description

Technical Field

[0001] The present invention relates to the field of material preparation, and more particularly to a method for preparing DNA-osmium-silver composite nanoparticles. Background Art

[0002] Exploring the enzyme-like properties of osmium nanoparticles is of great significance for expanding the applications of noble metal nanoparticles. Recent studies have shown that compared with other noble metal-based nanozymes, osmium nanomaterials exhibit higher peroxidase-like activity and negligible oxidase-like activity. This makes osmium nanomaterials a specific nanozyme with high peroxidase-like activity. However, the high cost limits the research and application of osmium nanoparticles. Preparing osmium-based composite nanomaterials has become a good choice to reduce costs. Summary of the Invention

[0003] The purpose of the present invention is to provide a method for preparing DNA-osmium-silver composite nanoparticles. The DNA-osmium-silver composite nanoparticles prepared by this method have silver nanoparticles more likely to be generated on the surface of osmium nanoparticles due to the adsorption of DNA on the surface of osmium nanoparticles, thereby obtaining osmium-silver composite nanoparticles with a high yield. In the market, the price of potassium osmate (K 2 OsCl 6 ) of the same manufacturer and the same quality is 8.3 times that of silver nitrate (AgNO 3 ). Therefore, the cost of the DNA-osmium-silver composite nanoparticles is significantly lower than that of the individual osmium nanoparticles. In summary, the DNA-osmium-silver composite nanoparticles combine the high peroxidase-like activity of osmium nanoparticles and the low price of silver nanoparticles, thereby reducing costs and the synergistic effect of the composite material greatly improves the peroxidase-like activity.

[0004] To achieve the above object, the technical solution adopted in the embodiments of the present invention is as follows:

[0005] A method for preparing DNA-osmium-silver composite nanoparticles,

[0006] First step: Prepare osmium nanoparticles.

[0007] In a dark environment, osmium nanoparticles are prepared from a potassium osmate (K 2 OsCl 6 ) solution and a sodium borohydride (NaBH 4 ) solution.

[0008] Second step: Add a deoxyribonucleic acid solution to obtain mixture 1.

[0009] Add a deoxyribonucleic acid solution to the osmium nanoparticle solution and stir well to obtain mixture 1.

[0010] Step 3: Generation of DNA-Osmium-Silver Composite Nanoparticles.

[0011] Add silver nitrate (AgNO 3 ) solution and sodium borohydride (NaBH 4 ) solution to the mixture 1, and stir to obtain DNA-osmium-silver composite nanoparticles.

[0012] In a preferred embodiment of the present invention,

[0013] During the preparation of the mixture 1 and osmium-silver composite nanoparticles, the reaction temperature is 20°C - 80°C.

[0014] In a preferred embodiment of the present invention,

[0015] During the preparation of the mixture 1, the concentration of potassium hexachloroosmate (K 2 OsCl 6 ) solution is 0.1 - 0.3 μmol / L, and the volume ratio of potassium hexachloroosmate (K 2 OsCl 6 ) to sodium borohydride (NaBH 4 ) solution is 100∶150.

[0016] In a preferred embodiment of the present invention,

[0017] During the preparation of the mixture 1 and osmium-silver composite nanoparticles, the concentration of sodium borohydride (NaBH 4 ) solution is 0.01 - 1 mol / L.

[0018] In a preferred embodiment of the present invention,

[0019] The concentration of the added deoxyribonucleic acid solution is 2 - 8 μmol / L.

[0020] In a preferred embodiment of the present invention,

[0021] The deoxyribonucleic acid base sequence is: CCC TTA ATC CCC TAT AAT AAA TTT TAA ATA TTATTT ATT AAT.

[0022] In a preferred embodiment of the present invention,

[0023] After adding the deoxyribonucleic acid solution, the stirring speed is 300 - 800 revolutions / min, and the stirring time is 5 - 15 min.

[0024] In a preferred embodiment of the present invention,

[0025] The volume ratio of the added deoxyribonucleic acid solution to the osmium nanoparticle solution is 1∶100 - 20∶100.

[0026] In a preferred embodiment of the present invention,

[0027] The concentration of silver nitrate (AgNO 3 ) solution is 0.1 - 0.3 μmol / L, and the volume ratio of silver nitrate (AgNO 3 ) solution to sodium borohydride (NaBH 4 ) solution is 100∶150.

[0028] The beneficial effects of the present invention are as follows:

[0029] The purpose of the present invention is to provide a method for preparing DNA - osmium - silver composite nanoparticles. The DNA - osmium - silver composite nanoparticles prepared by this method have silver nanoparticles more likely to be generated on the surface of osmium nanoparticles because of DNA adsorbed on the surface of osmium nanoparticles, thus obtaining osmium - silver composite nanoparticles with high yield. In the market, the price of potassium hexachloroosmate (K 2 OsCl 6 ) of the same manufacturer and the same quality is 8.3 times the price of silver nitrate (AgNO 3 ). Therefore, the cost of the DNA - osmium - silver composite nanoparticles is significantly lower than that of the individual osmium nanoparticles. In summary, the DNA - osmium - silver composite nanoparticles combine the high peroxidase - like activity of osmium nanoparticles and the low price of silver nanoparticles, thereby reducing the cost and the synergistic effect of the composite material greatly improves the peroxidase - like activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the embodiments.

[0031] Figure 1 FIG. is a transmission electron microscope photograph of the DNA - osmium - silver composite nanoparticles prepared in Example 1 of the present invention.

[0032] Figure 2 FIG. is a bar chart comparing the peroxidase - like activities of individual osmium nanoparticles, individual silver nanoparticles, and the DNA - osmium - silver composite nanoparticles prepared in Example 2 of the present invention.

[0033] Figure 3 FIG. shows a bar chart comparing the peroxidase - like activities of individual osmium nanoparticles, individual silver nanoparticles, and the DNA - osmium - silver composite nanoparticles prepared in Example 3 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0034] The embodiments of the present invention will be described in detail below in conjunction with examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. For those not specified in the examples, they are carried out under conventional conditions or conditions recommended by the manufacturer. Reagents or instruments not indicated by the manufacturer can be obtained as conventional products through commercial purchase.

[0035] In the description of the present invention, it should be noted that the terms "first", "second", etc. are only used for descriptive distinction and should not be construed as indicating or implying relative importance.

[0036] The preparation method of a DNA-osmium-silver composite nanoparticle according to an embodiment of the present invention will be specifically described below.

[0037] A preparation method of a DNA-osmium-silver composite nanoparticle provided by an embodiment of the present invention includes the following steps:

[0038] In a dark environment, osmium nanoparticles are prepared from a potassium hexachloroosmate (K 2 OsCl 6 ) solution and a sodium borohydride (NaBH 4 ) solution. Then, a deoxyribonucleic acid solution is added to the osmium nanoparticle solution and stirred well to obtain a mixture 1. A silver nitrate (AgNO 3 ) solution and a sodium borohydride (NaBH 4 ) solution are added to the mixture 1 and stirred to obtain the DNA-osmium-silver composite nanoparticle.

[0039] Furthermore, during the preparation of the mixture 1 and the osmium-silver composite nanoparticle, the reaction temperature is 20°C - 80°C.

[0040] Furthermore, during the preparation of the mixture 1, the concentration of the potassium hexachloroosmate (K 2 OsCl 6 ) solution is 0.1 - 0.3 μmol / L, and the volume ratio of the potassium hexachloroosmate (K 2 OsCl 6 ) to the sodium borohydride (NaBH 4 ) solution is 100∶150.

[0041] Furthermore, during the preparation of the mixture 1 and the osmium-silver composite nanoparticle, the concentration of the sodium borohydride (NaBH 4 ) solution is 0.01 - 1 mol / L.

[0042] Furthermore, the concentration of the added deoxyribonucleic acid solution is 2 - 8 μmol / L.

[0043] Further, the deoxyribonucleic acid base sequence is: CCC TTA ATC CCC TAT AAT AAA TTT TAAATA TTA TTT ATT AAT.

[0044] Further, after adding the deoxyribonucleic acid solution, the stirring speed is 300 - 800 revolutions per minute, and the stirring time is 5 - 15 minutes.

[0045] Further, the volume ratio of the added deoxyribonucleic acid solution to the osmium nanoparticle solution is 1∶100 - 20∶100.

[0046] Further, the concentration of silver nitrate (AgNO 3 ) solution is 0.1 - 0.3 μmol / L, and the volume ratio of silver nitrate (AgNO 3 ) solution to sodium borohydride (NaBH 4 ) solution is 100∶150.

[0047] The features and properties of the present invention will be further described in detail below in conjunction with embodiments:

[0048] Example 1

[0049] The DNA - osmium - silver composite nanoparticles provided in this example are prepared by the following steps:

[0050] At 30 °C, 1 mL of 0.1 μmol / L potassium hexachloroosmate (K 2 OsCl 6 ) solution and 1.5 mL of 1 mol / L sodium borohydride (NaBH 4 ) solution are used to prepare osmium nanoparticles. 25 μL of 2 μmol / L deoxyribonucleic acid solution is added to 500 μL of the prepared osmium nanoparticle solution, and stirred thoroughly at a stirring speed of 300 revolutions per minute to obtain mixture 1. 1 mL of 0.1 μmol / L silver nitrate (AgNO 3 ) solution and 1.5 mL of 1 mol / L sodium borohydride (NaBH 4 ) solution are added to mixture 1, and stirred continuously for 10 s to obtain DNA - osmium - silver composite nanoparticles.

[0051] Example 2

[0052] The DNA - osmium - silver composite nanoparticles provided in this example are prepared by the following steps:

[0053] At 40 °C, 2 mL of 0.2 μmol / L potassium hexachloroosmate (K 2 OsCl 6 ) solution and 3 mL of 0.5 mol / L sodium borohydride (NaBH4 ) The osmium nanoparticles were prepared by solution preparation. 40 μL of 5 μmol / L deoxyribonucleic acid solution was added to 700 μL of the prepared osmium nanoparticle solution, and stirred well at a stirring speed of 500 revolutions per minute to obtain mixture 1. 2 mL of 0.2 μmol / L silver nitrate (AgNO 3 ) solution and 3 mL of 0.5 mol / L sodium borohydride (NaBH 4 ) solution were added to mixture 1, and stirred continuously for 10 s to obtain DNA-osmium-silver composite nanoparticles.

[0054] Example 3

[0055] The DNA-osmium-silver composite nanoparticles provided in this example were prepared by the following steps:

[0056] At 50 °C, osmium nanoparticles were prepared from 1.5 mL of 0.3 μmol / L potassium hexachloroosmate (K 2 OsCl 6 ) solution and 2.25 mL of 0.8 mol / L sodium borohydride (NaBH 4 ) solution. 60 μL of 8 μmol / L deoxyribonucleic acid solution was added to 800 μL of the prepared osmium nanoparticle solution, and stirred well at a stirring speed of 800 revolutions per minute to obtain mixture 1. 1.5 mL of 0.3 μmol / L silver nitrate (AgNO 3 ) solution and 2.25 mL of 0.8 mol / L sodium borohydride (NaBH 4 ) solution were added to mixture 1, and stirred continuously for 10 s to obtain DNA-osmium-silver composite nanoparticles.

[0057] Experimental Example 1:

[0058] The particle size of the DNA-osmium-silver composite nanoparticles prepared in Example 1 was observed by transmission electron microscopy. It was observed that the nanoparticle size of the DNA-osmium-silver composite nanoparticles prepared in Example 1 was about 10 nm.

[0059] Figure 1 The transmission electron microscopy photograph of the DNA-osmium-silver composite nanoparticles prepared in Example 1 is shown.

[0060] Experimental Example 2:

[0061] The peroxidase-like activity of the DNA-osmium-silver composite nanoparticles prepared in Example 2 was tested. The test results showed that the enzyme-like activity ratios of the individual osmium nanoparticles, individual silver nanoparticles, and DNA-osmium-silver composite nanoparticles were 55∶40∶100.

[0062] Figure 2The bar chart showing the comparison of the peroxidase-like activities of individual osmium nanoparticles, individual silver nanoparticles, and the DNA-osmium-silver composite nanoparticles prepared in Example 2 of the present invention is presented.

[0063] Experimental Example III:

[0064] The peroxidase-like activity of the DNA-osmium-silver composite nanoparticles prepared in Example 3 was tested. The test results showed that the enzyme-like activity ratios of individual osmium nanoparticles, individual silver nanoparticles, and the DNA-osmium-silver composite nanoparticles were 60∶35∶110.

[0065] Figure 3 The bar chart showing the comparison of the peroxidase-like activities of individual osmium nanoparticles, individual silver nanoparticles, and the DNA-osmium-silver composite nanoparticles prepared in Example 3 of the present invention is presented.

[0066] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, various modifications and variations can be made to the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for preparing DNA-osmium-silver composite nanoparticles, characterized in that, In a dark environment, osmium nanoparticles are prepared from a potassium hexachloroosmate (K 2 OsCl 6 ) solution and a sodium borohydride (NaBH 4 ) solution. Then, deoxyribonucleic acid (DNA) is added to the osmium nanoparticle solution and stirred well to obtain mixture 1. Silver nitrate (AgNO 3 ) solution and sodium borohydride (NaBH 4 ) solution are added to mixture 1 and stirred to obtain DNA-osmium-silver composite nanoparticles.

2. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, During the preparation of the mixed solution 1 and the osmium-silver composite nanoparticles, the reaction temperature is 20°C - 80°C.

3. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, During the preparation of the mixed solution 1, the concentration of potassium hexachloroosmate (K 2 OsCl 6 ) solution is 0.1 - 0.3 μmol / L, and the volume ratio of the potassium hexachloroosmate (K 2 OsCl 6 ) solution to the sodium borohydride (NaBH 4 ) solution is 100∶150.

4. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, During the preparation of the mixed solution 1 and the osmium-silver composite nanoparticles, the concentration of the sodium borohydride (NaBH 4 ) solution is 0.01 - 1 mol / L for both.

5. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, The concentration of the added deoxyribonucleic acid solution is 2 - 8 μmol / L.

6. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, The deoxyribonucleic acid base sequence is: CCC TTA ATC CCC TAT AAT AAA TTT TAA ATA TTA TTTATT AAT.

7. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, After adding the deoxyribonucleic acid solution, the stirring speed is 300 - 800 revolutions / min, and the stirring time is 5 - 15 min.

8. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, The volume ratio of the added deoxyribonucleic acid solution to the osmium nanoparticle solution is 1∶100 - 20∶100.

9. The method for preparing DNA-osmium-silver composite nanoparticles according to claim 1, characterized in that, The concentration of silver nitrate (AgNO 3 ) solution is 0.1 - 0.3 μmol / L, and the volume ratio of silver nitrate (AgNO 3 ) solution to sodium borohydride (NaBH 4 ) solution is 100∶150.

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