Stevia rebaudiana plants with enhanced nutritional content

Abstract

The present invention provides a stevia plant having a genotype of C / C at a position corresponding to position 37 of SEQ ID NO: 1. The present invention also provides methods of making the stevia plant, and extracts derived from the plant.

Description

TECHNICAL FIELD

[0001] The present application relates to a stevia plant having a genotype of C / C at a position corresponding to position 37 of SEQ ID NO: 1, a production method thereof, a screening method, and the like. BACKGROUND

[0002] Stevia is a perennial plant of the Asteraceae family, which is native to Paraguay in South America. Stevia contains various sweet components having several tens to several hundreds of times the sweetness of sugar, and these sweet components are used as a natural sweetener by being extracted (Patent Document 1). However, there are still many unknown parts regarding the genetic information of stevia or the control of the in vivo phenomenon and what genes are involved.

[0003] PATENT DOCUMENT

[0004] Patent Document 1: WO2018 / 124142 SUMMARY

[0005] The genetic information of stevia is further pursued to be elucidated.

[0006] The present application provides a stevia plant body rich in nutritional components, a production method thereof, a screening method, and the like.

[0007] In one aspect, the present application provides the following.

[0008] [1] A stevia plant body, characterized by having a genotype of C / C at a position corresponding to position 37 of SEQ ID NO: 1.

[0009] [2] The plant body according to [1], characterized by containing more at least one kind of nutritional components selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, rebaudioside D, and rebaudioside M, and / or having a lower expression amount of FRO2 than a control stevia plant body having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1.

[0010] [3] The plant body according to [1] or [2], characterized by being a non-transgenic plant body.

[0011] [4] A tissue, tissue culture, or cell, characterized by being a tissue, tissue culture, or cell of the plant body according to any one of [1] to [3].

[0012] [5] The tissue, tissue culture, or cell according to [4], characterized by being selected from seeds, embryos, meristem cells, pollen, leaves, roots, root tips, petals, protoplasts, sections of leaves, and calli.

[0013] [6] A method for producing a stevia plant body, which is a stevia plant body having more of at least one nutrient selected from the group consisting of iron, zinc, phosphorus, copper, molybdenum, an amino acid, rebaudioside D, and rebaudioside M, and / or a lower expression amount of FRO2 than a control stevia plant body having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1, characterized by comprising a step of crossing the plant body according to any one of [1] to [3] with a second stevia plant body.

[0014] [7] The method according to [6], characterized in that the second plant body is the plant body according to any one of [1] to [3].

[0015] [8] An extract of the plant body according to any one of [1] to [3], the tissue according to [4] or [5], or the tissue culture or cells, characterized by containing at least one nutrient selected from the group consisting of iron, zinc, phosphorus, copper, molybdenum, an amino acid, rebaudioside D, and rebaudioside M.

[0016] [9] A method for producing an extract containing at least one nutrient selected from the group consisting of iron, zinc, phosphorus, copper, molybdenum, an amino acid, rebaudioside D, and rebaudioside M, characterized by comprising a step of obtaining the extract from the plant body according to any one of [1] to [3], the tissue according to [4] or [5], or the tissue culture or cells.

[0017]

[10] A method for producing a food, a sweetener composition, a flavor, or a pharmaceutical product, characterized by comprising a step of providing the extract of the plant body according to any one of [1] to [3], the extract of the tissue, the tissue culture, or the cells according to [4] or [5], or the extract according to [8], and,

[0018] a step of adding the extract to a raw material of the food, the sweetener composition, the flavor, or the pharmaceutical product.

[0019]

[11] A herbal tea product, characterized by containing a dried product of the plant body according to any one of [1] to [3].

[0020]

[12] A method for screening the stevia plant body according to any one of [1] to [3], characterized by comprising a step of detecting whether the genotype at a position corresponding to position 37 of SEQ ID NO: 1 is C / C from a stevia plant body to be tested.

[0021]

[13] The method according to

[12] , characterized in that the step of detecting the genetic trait is performed using sequencing, the dCAPS method, or the TaqMan PCR method.

[0022]

[14] The method according to

[12] or

[13] , characterized by further comprising a step of measuring the content of at least one of the nutrient components selected from the group consisting of iron, zinc, phosphorus, copper, molybdenum, amino acids, rebaudioside D, and rebaudioside M in the stevia plant tissue to be examined.

[0023]

[15] A screening kit for the stevia plant body according to any one of [1] to [3], characterized by containing a reagent for detecting whether the genotype at the position corresponding to position 37 of SEQ ID NO: 1 is C / C.

[0024]

[16] The kit according to

[15] , characterized in that the reagent contains primers and / or probes for dCAPS method or TaqMan PCR method.

[0025]

[17] A screening method for a plant body having a high content of at least one of the nutrient components selected from the group consisting of iron, zinc, phosphorus, copper, molybdenum, amino acids, rebaudioside D, and rebaudioside M, characterized by comprising a step of measuring the expression amount of FRO2.

[0026] According to the present application, a stevia plant body rich in nutrient components can be obtained, and a method for producing such a plant body, leaves obtained from such a plant body, and foods or beverages and the like containing an extract obtained from such leaves can be provided. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 A graph showing the variation site (position 37) in the base sequence of SEQ ID NO: 1. The boxed base is the base (C or T) of the variation site.

[0028] Figure 2 A graph showing the contents of RebD and RebM in each individual of genotypes C / C or C / T.

[0029] Figure 3 A graph showing the expression amount of the FRO2 gene in individuals of genotypes C / C or C / T.

[0030] Figure 4 A graph showing the ratio of the average content of each mineral component in individuals of genotype C / C to the average content of each mineral component in individuals of genotype C / T, when the average content of each mineral component in individuals of genotype C / T is taken as 1.

[0031] Figure 5 A graph showing the ratio of the average content of each amino acid in individuals of genotype C / C to the average content of each amino acid in individuals of genotype C / T, when the average content of each amino acid in individuals of genotype C / T is taken as 1.

[0032] Figure 6 A graph showing the correlation between the total content of RebD and RebM and the expression amount of FRO2.

[0033] Figure 7 A graph for comparing the total content of RebD and RebM in the individual group ranked in the lower 15% of FRO2 expression amount and the individual group ranked in the upper 15% of FRO2 expression amount.

[0034] Figure 8 A graph for showing the correlation of the ratio of the total content of RebD and RebM to the TSG content (RebDM / TSG) to the FRO2 expression amount. DETAILED DESCRIPTION

[0035] The present application is described in detail below. The following embodiments are examples for illustrating the present application, and the present application is not limited only to these embodiments. The present application can be implemented in various ways without departing from the gist thereof.

[0036] In addition, all the documents and publications, patent publications, and other patent documents cited in this specification are incorporated by reference into this specification. Furthermore, this specification contains the contents described in the specification and drawings of Japanese Patent Application (Japanese Patent Application No. 2021-069151) on which the priority of this application is based, filed on April 15, 2021.

[0037] 1. A stevia plant in which the genotype of the position corresponding to position 37 of SEQ ID NO: 1 is C / C

[0038] The present application provides a stevia plant (hereinafter, sometimes referred to as “the plant of the present application” or “the stevia plant of the present application”), characterized in that the genotype of the position corresponding to position 37 of SEQ ID NO: 1 is C / C (i.e., homozygous for the allele in which the base at the position corresponding to position 37 of SEQ ID NO: 1 is C). Hereinafter, the case where the genotype of the position corresponding to position 37 of SEQ ID NO: 1 is C / C is sometimes referred to as “the genetic trait of the present application”.

[0039] Stevia is a plant having the scientific name of Stevia Rebaudiana Bertoni.

[0040] The "position corresponding to the 37th position of SEQ ID NO: 1" means the 37th position from the 5' side of the portion of the genome having the same base sequence as SEQ ID NO: 1 when the genome has the portion having the same base sequence as SEQ ID NO: 1. On the other hand, when the genome of the stevia plant has a portion having a base sequence different from but corresponding to SEQ ID NO: 1, the "position corresponding to the 37th position of SEQ ID NO: 1" does not necessarily coincide with the 37th position from the 5' side of the portion corresponding to SEQ ID NO: 1, but can be determined by taking into account the base sequence around the 37th position of SEQ ID NO: 1, etc. For example, the "position corresponding to the 37th position of SEQ ID NO: 1" in the genome of the stevia plant can be determined by alignment analysis of the base sequence of the portion of the genome of the stevia plant corresponding to SEQ ID NO: 1 and the base sequence of SEQ ID NO: 1.

[0041] For example, when the genome of the stevia plant has a portion having the same base sequence as SEQ ID NO: 1, the "position corresponding to the 37th position of SEQ ID NO: 1" is the 37th position from the 5' side of the portion of the genome having the same base sequence as SEQ ID NO: 1. On the other hand, when the genome of the stevia plant has a portion having a base sequence different from but corresponding to SEQ ID NO: 1, since the genome does not have a portion having the same base sequence as SEQ ID NO: 1, the "position corresponding to the 37th position of SEQ ID NO: 1" does not necessarily coincide with the 37th position from the 5' side of the portion corresponding to SEQ ID NO: 1, but can be determined by taking into account the base sequence around the 37th position of SEQ ID NO: 1, etc. For example, the "position corresponding to the 37th position of SEQ ID NO: 1" in the genome of the stevia plant can be determined by alignment analysis of the base sequence of the portion of the genome of the stevia plant corresponding to SEQ ID NO: 1 and the base sequence of SEQ ID NO: 1.

[0042] The "portion consisting of a base sequence equivalent to SEQ ID NO: 1" means, for example, a portion consisting of a base sequence having 60% or more, 70% or more, 75% or more, 80% or more, 81% or more, 82% or more, 83% or more, 84% or more, 85% or more, 86% or more, 87% or more, 88% or more, 89% or more, 90% or more, 91% or more, 92% or more, 93% or more, 94% or more, 95% or more, 96% or more, 97% or more, 98% or more, 98.1% or more, 98.4% or more, 98.7% or more, 99% or more, 99.2% or more, 99.5% or more, or 99.8% or more of sequence identity to the base sequence of SEQ ID NO: 1.

[0043] In one embodiment, the "portion consisting of a base sequence equivalent to SEQ ID NO: 1" includes a portion of the genome of a stevia plant that can be amplified by PCR using a forward primer that hybridizes to a portion of 15 to 25 bases in length from the 5' end of SEQ ID NO: 1 and a reverse primer that hybridizes to a portion of 15 to 25 bases in length from the 3' end of SEQ ID NO: 1.

[0044] In one embodiment, the "portion consisting of a base sequence equivalent to SEQ ID NO: 1" includes a portion of the genome of a stevia plant that can be amplified by PCR using a forward primer that hybridizes to a portion of 15 to 25 bases in length from the 5' end of SEQ ID NO: 1 and a reverse primer that hybridizes to a portion of 15 to 25 bases in length from the 3' end of SEQ ID NO: 1.

[0045] In one embodiment, the "allele in which the base at the position equivalent to position 37 of SEQ ID NO: 1 is C" includes the base sequence of SEQ ID NO: 4, 5, 6, or 20.

[0046] Here, the position equivalent to position 37 of SEQ ID NO: 1 is sometimes referred to as the "polymorphic site of the present invention" or the "variant site of the present invention". Furthermore, the mutation from T to C at the position equivalent to position 37 of SEQ ID NO: 1 is sometimes referred to as the "polymorphism of the present invention" or the "variant of the present invention". Figure 1 Position 37 of SEQ ID NO: 1 is shown below.

[0047] The above genetic trait can be detected by PCR, TaqMan PCR, sequencing, microarray, Invader, TILLING, RAD (random amplified polymorphic DNA), RFLP (restriction fragment length polymorphism), PCR-SSCP, AFLP (amplified fragment length polymorphism), SSLP (simple sequence length polymorphism), CAPS (cleaved amplified polymorphic sequence), dCAPS (derived cleaved amplified polymorphic sequence), ASO (allele specific oligonucleotide), ARMS, DGGE (denaturing gradient gel electrophoresis), CCM (chemical cleavage of mismatch), DOL, MALDI-TOF / MS, TDI, Locus (labeled probe excess), Molecular Beacon, DASH (dynamicallele specific hybridization), UCAN, ECA, PINPOINT, PROBE (primer oligobase extension), VSET (very short extension), Survivor assay, Sniper assay, Luminex assay, GOOD, LCx, SNaPshot, Mass ARRAY, Pyrosequences, SNP-IT, melting curve analysis, etc., but the detection method is not limited to these.

[0048] In a specific manner, the genetic trait of the present application can be detected based on the following combination of primer sets and restriction enzymes. When a candidate plant has the genetic trait of the present application, for example, PCR amplification is performed using a forward primer having the base sequence shown in SEQ ID NO: 8 and a reverse primer having the base sequence shown in SEQ ID NO: 9 with respect to the genomic DNA of the candidate plant, and even if treatment based on the restriction enzyme Afl III is performed with respect to the resulting PCR product (about 220 bp long, for example, SEQ ID NO: 9), only a band (for example, SEQ ID NO: 9) of about 220 bp long is obtained. On the other hand, when PCR amplification is performed, for example, a PCR product (about 220 bp long) of SEQ ID NO: 10 is obtained, and by the restriction enzyme Afl III, a restriction enzyme-treated product (for example, SEQ ID NO: 11) of about 34 bp and a restriction enzyme-treated product (for example, SEQ ID NO: 12) of about 186 bp are produced, the candidate plant does not have the genetic trait of the present application.

[0049] With respect to the bp length described above, the so-called "about" means ± 5 bp. The restriction enzyme treatment can be performed in accordance with the conditions recommended by the seller of each restriction enzyme used.

[0050] In one aspect, with respect to the plant body of the present application, the plant body has more of at least one nutrient selected from the group consisting of minerals (e.g., iron, zinc, phosphorus, copper, molybdenum, etc.), amino acids (e.g., alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, etc.), Rebaudioside D, and Rebaudioside M (hereinafter, sometimes referred to as "Chemical Feature A of the present application") as compared to a control stevia plant body having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1. By "has more" is meant, for example, that the content of the above-mentioned component (e.g., content in dried leaves) is more than that of the control stevia plant body when cultivated under the same cultivation conditions. More specifically, for example, the content of the above-mentioned component in dried leaves can be more than 1-fold, 1.01-fold or more, 1.02-fold or more, 1.03-fold or more, 1.04-fold or more, 1.05-fold or more, 1.06-fold or more, 1.07-fold or more, 1.08-fold or more, 1.09-fold or more, 1.1-fold or more, 1.2-fold or more, 1.3-fold or more, 1.4-fold or more, 1.5-fold or more, 1.6-fold or more, 1.7-fold or more, 1.8-fold or more, 1.9-fold or more, 2.0-fold or more, 2.1-fold or more, 2.2-fold or more, 2.3-fold or more, 2.4-fold or more, 2.5-fold or more, 2.6-fold or more, 2.7-fold or more, 2.8-fold or more, 2.9-fold or more, 3.0-fold or more, 3.1-fold or more, 3.2-fold or more, 3.3-fold or more, 3.4-fold or more, 3.5-fold or more, 3.6-fold or more, 3.7-fold or more, 3.8-fold or more, 3.9-fold or more, 4.0-fold or more, 4.1-fold or more, 4.2-fold or more, 4.3-fold or more, 4.4-fold or more, 4.5-fold or more, 4.6-fold or more, 4.7-fold or more, 4.8-fold or more, 4.9-fold or more, 5.0-fold or more, 10-fold or more, 15-fold or more, 20-fold or more, 25-fold or more, 30-fold or more, 35-fold or more, 40-fold or more, 45-fold or more, 50-fold or more, 55-fold or more, 60-fold or more, 65-fold or more, 70-fold or more, 75-fold or more, 80-fold or more, 85-fold or more, 90-fold or more, 95-fold or more, or 100-fold or more than that of the control stevia plant body when cultivated under the same cultivation conditions. The content to be compared is the average value of a plurality of individuals. In addition, dried leaves refer to leaves in which the water content is reduced to 3 to 4% by weight by drying fresh leaves of the stevia plant body of the present application. In addition, Rebaudioside is sometimes abbreviated as "Reb." or "Reb." in the present specification. For example, Rebaudioside D is sometimes abbreviated as "Reb. D" or "RebD".

[0051] In some aspects, the plant body of the present application has a higher content of minerals in dried leaves than a control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature Al-a of the present application"). For example, the plant body of the present application can have a content of minerals in dried leaves that is 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.1 times or more, 4.2 times or more, 4.3 times or more, 4.4 times or more, 4.5 times or more, 4.6 times or more, 4.7 times or more, 4.8 times or more, 4.9 times or more, 5.0 times or more, 5.1 times or more, 5.2 times or more, 5.3 times or more, 5.4 times or more, 5.5 times or more, 5.6 times or more, 5.7 times or more, 5.8 times or more, 5.9 times or more, 6.0 times or more, 6.1 times or more, 6.2 times or more, 6.3 times or more, 6.4 times or more, 6.5 times or more, 6.6 times or more, 6.7 times or more, 6.8 times or more, 6.9 times or more, or 7.0 times or more than a control stevia plant body when cultivated under the same cultivation conditions.

[0052] In one embodiment, the content of amino acids (free amino acids) in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-b of the present application"). For example, the content of amino acids in the dried leaves of the plant body of the present application can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, more than 2.6 times, more than 2.7 times, more than 2.8 times, more than 2.9 times, more than 3.0 times, more than 3.1 times, more than 3.2 times, more than 3.3 times, more than 3.4 times, more than 3.5 times, more than 3.6 times, more than 3.7 times, more than 3.8 times, more than 3.9 times, more than 4.0 times, more than 4.1 times, more than 4.2 times, more than 4.3 times, more than 4.4 times, more than 4.5 times, more than 5.0 times, more than 10 times, more than 15 times, more than 20 times, more than 25 times, more than 30 times, more than 35 times, more than 40 times, more than 45 times, more than 50 times, more than 55 times, more than 60 times, more than 65 times, more than 70 times, more than 75 times, more than 80 times, more than 85 times, more than 90 times, more than 95 times, or more than 100 times that in the control stevia plant body when cultivated under the same cultivation conditions.

[0053] In one embodiment, the content of iron in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-1 of the present application"). For example, the content of iron in the dried leaves of the plant body of the present application can be more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, or more than 2.5 times that in the control stevia plant body when cultivated under the same cultivation conditions.

[0054] In a particular mode, in terms of the plant body of the present application, the content of zinc in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-2 of the present application"). For example, in terms of the plant body of the present application, the content of zinc in the dried leaf can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, or more than 2.0 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0055] In a particular mode, in terms of the plant body of the present application, the content of phosphorus (inorganic phosphorus) in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-3 of the present application"). For example, in terms of the plant body of the present application, the content of phosphorus in the dried leaf can be more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, or more than 2.6 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0056] In a particular mode, in terms of the plant body of the present application, the content of copper in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-4 of the present application"). For example, in terms of the plant body of the present application, the content of copper in the dried leaf can be more than 3.0 times, more than 3.1 times, more than 3.2 times, more than 3.3 times, more than 3.4 times, more than 3.5 times, more than 3.6 times, more than 3.7 times, more than 3.8 times, more than 3.9 times, more than 4.0 times, more than 4.1 times, more than 4.2 times, more than 4.3 times, more than 4.4 times, more than 4.5 times, more than 4.6 times, more than 4.7 times, more than 4.8 times, more than 4.9 times, or more than 5.0 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0057] In a particular manner, in terms of the plant body of the present application, the content of molybdenum in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-5 of the present application"). For example, in terms of the plant body of the present application, the content of molybdenum in the dried leaf can be 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.1 times or more, 4.2 times or more, 4.3 times or more, 4.4 times or more, 4.5 times or more, 4.6 times or more, 4.7 times or more, 4.8 times or more, 4.9 times or more, 5.0 times or more, 5.1 times or more, 5.2 times or more, 5.3 times or more, 5.4 times or more, 5.5 times or more, 5.6 times or more, 5.7 times or more, 5.8 times or more, 5.9 times or more, 6.0 times or more, 6.1 times or more, 6.2 times or more, 6.3 times or more, 6.4 times or more, 6.5 times or more, 6.6 times or more, 6.7 times or more, 6.8 times or more, 6.9 times or more, or 7.0 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0058] In a particular manner, in terms of the plant body of the present application, the content of molybdenum in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-5 of the present application"). For example, in terms of the plant body of the present application, the content of molybdenum in the dried leaf can be 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.1 times or more, 4.2 times or more, 4.3 times or more, 4.4 times or more, 4.5 times or more, 4.6 times or more, 4.7 times or more, 4.8 times or more, 4.9 times or more, 5.0 times or more, 5.1 times or more, 5.2 times or more, 5.3 times or more, 5.4 times or more, 5.5 times or more, 5.6 times or more, 5.7 times or more, 5.8 times or more, 5.9 times or more, 6.0 times or more, 6.1 times or more, 6.2 times or more, 6.3 times or more, 6.4 times or more, 6.5 times or more, 6.6 times or more, 6.7 times or more, 6.8 times or more, 6.9 times or more, or 7.0 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0059] In a particular aspect, the content of arginine in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-7 of the present application"). For example, the content of arginine in the dried leaves of the plant body of the present application can be 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 5.0 times or more, 10 times or more, 15 times or more, 20 times or more, 25 times or more, 30 times or more, 35 times or more, 40 times or more, 45 times or more, 50 times or more, 55 times or more, 60 times or more, 65 times or more, 70 times or more, 75 times or more, 80 times or more, 85 times or more, 90 times or more, 95 times or more, or 100 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0060] In a particular aspect, the content of asparagine in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-8 of the present application"). For example, the content of asparagine in the dried leaves of the plant body of the present application can be 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.8 times or more, 4.0 times or more, 4.3 times or more, 4.5 times or more, 4.8 times or more, 5.0 times or more, 5.3 times or more, 5.5 times or more, 5.8 times or more, 6.0 times or more, 6.3 times or more, 6.5 times or more, 6.8 times or more, 7.0 times or more, 7.3 times or more, 7.5 times or more, 7.8 times or more, or 8.0 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0061] In a particular manner, in the case of the plant body of the present application, the content of aspartic acid in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-9 of the present application"). For example, in the case of the plant body of the present application, the content of aspartic acid in the dried leaf can be 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 4.0 times or more, 5.0 times or more, 10 times or more, 15 times or more, 20 times or more, 25 times or more, 30 times or more, 35 times or more, 40 times or more, 45 times or more, 50 times or more, 55 times or more, 60 times or more, 65 times or more, 70 times or more, 75 times or more, 80 times or more, 85 times or more, 90 times or more, 95 times or more, or 100 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0062] In a particular manner, in the case of the plant body of the present application, the content of aspartic acid in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-9 of the present application"). For example, in the case of the plant body of the present application, the content of aspartic acid in the dried leaf can be 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 4.0 times or more, 5.0 times or more, 10 times or more, 15 times or more, 20 times or more, 25 times or more, 30 times or more, 35 times or more, 40 times or more, 45 times or more, 50 times or more, 55 times or more, 60 times or more, 65 times or more, 70 times or more, 75 times or more, 80 times or more, 85 times or more, 90 times or more, 95 times or more, or 100 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0063] In a particular aspect, the content of glycine in the dried leaf of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-11 of the present application"). For example, the content of glycine in the dried leaf of the plant body of the present application can be 1.0 times or more, 1.01 times or more, 1.02 times or more, 1.03 times or more, 1.04 times or more, 1.05 times or more, 1.06 times or more, 1.07 times or more, 1.08 times or more, 1.09 times or more, 1.1 times or more, 1.2 times or more, 1.3 times or more, 1.4 times or more, 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 3.0 times or more, 4.0 times or more, 5.0 times or more, 10 times or more, 15 times or more, 20 times or more, 25 times or more, 30 times or more, 35 times or more, 40 times or more, 45 times or more, 50 times or more, 55 times or more, 60 times or more, 65 times or more, 70 times or more, 75 times or more, 80 times or more, 85 times or more, 90 times or more, 95 times or more, or 100 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0064] In a particular aspect, the content of histidine in the dried leaf of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-12 of the present application"). For example, the content of histidine in the dried leaf of the plant body of the present application can be more than 1.0 times, 1.01 times or more, 1.02 times or more, 1.03 times or more, 1.04 times or more, 1.05 times or more, 1.06 times or more, 1.07 times or more, 1.08 times or more, 1.09 times or more, 1.1 times or more, 1.2 times or more, 1.3 times or more, 1.4 times or more, 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, or 3.0 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0065] In a particular mode, in the case of the plant body of the present application, the content of isoleucine in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-13 of the present application"). For example, in the case of the plant body of the present application, the content of isoleucine in the dried leaf can be more than 1.0 times, 1.01 times or more, 1.02 times or more, 1.03 times or more, 1.04 times or more, 1.05 times or more, 1.06 times or more, 1.07 times or more, 1.08 times or more, 1.09 times or more, 1.1 times or more, 1.2 times or more, 1.3 times or more, 1.4 times or more, 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, or 3.0 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0066] In a particular mode, in the case of the plant body of the present application, the content of leucine in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-14 of the present application"). For example, in the case of the plant body of the present application, the content of leucine in the dried leaf can be more than 1.0 times, 1.01 times or more, 1.02 times or more, 1.03 times or more, 1.04 times or more, 1.05 times or more, 1.06 times or more, 1.07 times or more, 1.08 times or more, 1.09 times or more, 1.1 times or more, 1.2 times or more, 1.3 times or more, 1.4 times or more, 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, or 2.0 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0067] In a particular aspect, the content of lysine in the dried leaf of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-15 of the present application"). For example, the content of lysine in the dried leaf of the plant body of the present application can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, or more than 2.6 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0068] In a particular aspect, the content of lysine in the dried leaf of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-15 of the present application"). For example, the content of lysine in the dried leaf of the plant body of the present application can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, or more than 2.6 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0069] In a particular manner, in the case of the plant body of the present application, the content of proline in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-17 of the present application"). For example, in the case of the plant body of the present application, the content of proline in the dried leaf can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, more than 2.6 times, or more than 2.7 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0070] In a particular manner, in the case of the plant body of the present application, the content of serine in the dried leaf is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-18 of the present application"). For example, in the case of the plant body of the present application, the content of serine in the dried leaf can be more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, more than 2.6 times, more than 2.7 times, more than 2.8 times, more than 2.9 times, more than 3.0 times, more than 3.1 times, more than 3.2 times, more than 3.3 times, more than 3.4 times, more than 3.5 times, more than 3.6 times, more than 3.7 times, more than 3.8 times, more than 3.9 times, more than 4.0 times, more than 5.0 times, more than 6.0 times, more than 7.0 times, more than 8.0 times, more than 9.0 times, more than 10 times, more than 11 times, more than 12 times, more than 13 times, more than 14 times, more than 15 times, more than 16 times, more than 17 times, more than 18 times, more than 19 times, more than 20 times, more than 21 times, more than 22 times, more than 23 times, more than 24 times, more than 25 times, more than 26 times, or more than 27 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0071] In a particular aspect, the content of threonine in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-19 of the present application"). For example, the content of threonine in the dried leaves of the plant body of the present application can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, or more than 2.0 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0072] In a particular aspect, the content of tyrosine in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-20 of the present application"). For example, the content of tyrosine in the dried leaves of the plant body of the present application can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, more than 2.2 times, more than 2.3 times, more than 2.4 times, more than 2.5 times, more than 2.6 times, or more than 2.7 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0073] In a particular aspect, the content of valine in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical characteristic A1-21 of the present application"). For example, the content of valine in the dried leaves of the plant body of the present application can be more than 1.0 times, more than 1.01 times, more than 1.02 times, more than 1.03 times, more than 1.04 times, more than 1.05 times, more than 1.06 times, more than 1.07 times, more than 1.08 times, more than 1.09 times, more than 1.1 times, more than 1.2 times, more than 1.3 times, more than 1.4 times, more than 1.5 times, more than 1.6 times, more than 1.7 times, more than 1.8 times, more than 1.9 times, more than 2.0 times, more than 2.1 times, or more than 2.2 times the content in the control stevia plant body when cultivated under the same cultivation conditions.

[0074] In a particular aspect, the content of RebD in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-22 of the present application"). For example, the content of RebD in the dried leaves of the plant body of the present application can be 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.1 times or more, 4.2 times or more, 4.3 times or more, 4.4 times or more, 4.5 times or more, 5.0 times or more, 6.0 times or more, 7.0 times or more, 8.0 times or more, 9.0 times or more, 10 times or more, 11 times or more, 12 times or more, 13 times or more, 14 times or more, 15 times or more, 16 times or more, 17 times or more, 18 times or more, 19 times or more, 20 times or more, 21 times or more, 22 times or more, 23 times or more, 24 times or more, 25 times or more, 26 times or more, 27 times or more, or 28 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0075] In a particular aspect, the content of RebM in the dried leaves of the plant body of the present application is more than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-23 of the present application"). For example, the content of RebM in the dried leaves of the plant body of the present application can be 1.2 times or more, 1.3 times or more, 1.4 times or more, 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 5.0 times or more, 7.0 times or more, 9.0 times or more, 11 times or more, 13 times or more, 15 times or more, 17 times or more, 19 times or more, 21 times or more, 23 times or more, 25 times or more, 27 times or more, 29 times or more, 31 times or more, 33 times or more, 35 times or more, 37 times or more, 39 times or more, 41 times or more, 43 times or more, 45 times or more, 47 times or more, 49 times or more, 51 times or more, or 53 times or more than that in the control stevia plant body when cultivated under the same cultivation conditions.

[0076] In a particular aspect, the plant body of the present application has a higher total content of RebD and RebM in dried leaves than a control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-24 of the present application"). For example, the plant body of the present application can have a total content of RebD and RebM in dried leaves that is 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.5 times or more, 5.0 times or more, 6.0 times or more, 7.0 times or more, 8.0 times or more, 9.0 times or more, 10 times or more, 11 times or more, 12 times or more, 13 times or more, 14 times or more, 15 times or more, 16 times or more, 17 times or more, 18 times or more, 19 times or more, 20 times or more, 21 times or more, 22 times or more, 23 times or more, 24 times or more, 25 times or more, 26 times or more, or 27 times or more than the content in a control stevia plant body when cultivated under the same cultivation conditions.

[0077] In a particular aspect, the plant body of the present application has a higher weight ratio of RebD to total steviol glycoside (RebD / TSG) in dried leaves than a control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature A1-25 of the present application"). For example, the plant body of the present application can have a RebD / TSG in dried leaves that is 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.1 times or more, 4.2 times or more, 4.3 times or more, 4.4 times or more, 4.5 times or more, 4.6 times or more, 4.7 times or more, 4.8 times or more, 4.9 times or more, 5.0 times or more, 7.0 times or more, 9.0 times or more, 11 times or more, 13 times or more, 15 times or more, 17 times or more, 19 times or more, 21 times or more, 23 times or more, 25 times or more, 27 times or more, 29 times or more, 31 times or more, 33 times or more, 35 times or more, 37 times or more, 39 times or more, 41 times or more, 43 times or more, or 45 times or more than the RebD / TSG in a control stevia plant body when cultivated under the same cultivation conditions.

[0078] The so-called TSG is a total of the determinable steviol glycosides, excluding unknown steviol glycosides or steviol glycosides present in an amount less than the detection limit. The TSG is preferably an arbitrary combination of two or more selected from the group consisting of RebA, RebB, RebC, RebD, RebE, RebF, RebG, RebI, RebJ, RebK, RebM, RebN, RebO, RebQ, RebR, ducoside A, rubusoside, steviol monoside, steviol diside, and stevioside. In a particular manner, the TSG consists of RebA, RebB, RebC, RebD, RebE, RebF, RebG, RebM, RebN, and stevioside.

[0079] In a particular manner, in the case of the plant body of the present application, the weight ratio of RebM to TSG (RebM / TSG) in the dried leaf is larger than that in the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature A1-26 of the present application"). For example, in the case of the plant body of the present application, the RebM / TSG in the dried leaf when cultivated under the same cultivation conditions can be 1.5 times or more, 1.6 times or more, 1.7 times or more, 1.8 times or more, 1.9 times or more, 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 4.0 times or more, 4.5 times or more, 5.0 times or more, 10 times or more, 15 times or more, 20 times or more, 25 times or more, 30 times or more, 35 times or more, 40 times or more, 45 times or more, 50 times or more, 55 times or more, 60 times or more, 65 times or more, 70 times or more, 75 times or more, 80 times or more, 85 times or more, 90 times or more, 95 times or more, or 100 times or more than that in the control stevia plant body.

[0080] In a specific aspect, the plant body of the present application has a larger ratio of the total of RebD and RebM in the dried leaves to the weight of TSG (RebDM / TSG) than that of the control stevia plant body when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "Chemical feature Al-27 of the present application"). For example, the plant body of the present application can have RebDM / TSG in the dried leaves that is 2.0 times or more, 2.1 times or more, 2.2 times or more, 2.3 times or more, 2.4 times or more, 2.5 times or more, 2.6 times or more, 2.7 times or more, 2.8 times or more, 2.9 times or more, 3.0 times or more, 3.1 times or more, 3.2 times or more, 3.3 times or more, 3.4 times or more, 3.5 times or more, 3.6 times or more, 3.7 times or more, 3.8 times or more, 3.9 times or more, 4.0 times or more, 4.1 times or more, 4.2 times or more, 4.3 times or more, 4.4 times or more, 4.5 times or more, 4.6 times or more, 4.7 times or more, 4.8 times or more, 4.9 times or more, 5.0 times or more, 6.0 times or more, 8.0 times or more, 10 times or more, 12 times or more, 14 times or more, 16 times or more, 18 times or more, 20 times or more, 22 times or more, 24 times or more, 26 times or more, 28 times or more, 30 times or more, 32 times or more, 34 times or more, 36 times or more, 38 times or more, or 40 times or more than that of the control stevia plant body when cultivated under the same cultivation conditions.

[0081] The plant body of the present application can also have two or more of the above chemical features in combination. For example, the plant body of the present application can have chemical features Al-a and Al-b of the present application, and can also have at least two of chemical features Al-1 to Al-27 (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty, twenty-one, twenty-two, twenty-three, twenty-four, twenty-five, twenty-six, or twenty-seven of chemical features Al-1 to Al-27), for example, the content of minerals in the dried leaves can be 2.2 to 4.4 times or more, the content of free amino acids can be 1.1 to 3.8 times or more, and the total content of RebD and RebM can be 2.4 times or more than that of the control stevia plant body when cultivated under the same cultivation conditions. In some aspects, the plant body of the present application has at least two or more of features (Al-1') to (Al-27') (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty, twenty-one, twenty-two, twenty-three, twenty-four, twenty-five, twenty-six, or twenty-seven of features (Al-1') to (Al-27')):

[0082] (A1-1') when cultivated under the same cultivation conditions, the content of iron in the dried leaves is 2.2 times or more compared to a control stevia plant,

[0083] (A1-2') when cultivated under the same cultivation conditions, the content of zinc in the dried leaves is 1.7 times or more compared to a control stevia plant,

[0084] (A1-3') when cultivated under the same cultivation conditions, the content of phosphorus in the dried leaves is 2.2 times or more compared to a control stevia plant,

[0085] (A1-4') when cultivated under the same cultivation conditions, the content of copper in the dried leaves is 4.2 times or more compared to a control stevia plant,

[0086] (A1-5') when cultivated under the same cultivation conditions, the content of molybdenum in the dried leaves is 4.4 times or more compared to a control stevia plant,

[0087] (A1-6') when cultivated under the same cultivation conditions, the content of alanine in the dried leaves is 1.2 times or more compared to a control stevia plant,

[0088] (A1-7') when cultivated under the same cultivation conditions, the content of arginine in the dried leaves is 3.8 times or more compared to a control stevia plant,

[0089] (A1-8') when cultivated under the same cultivation conditions, the content of asparagine in the dried leaves is 3.1 times or more compared to a control stevia plant,

[0090] (A1-9') when cultivated under the same cultivation conditions, the content of aspartic acid in the dried leaves is 2.2 times or more compared to a control stevia plant,

[0091] (A1-10') when cultivated under the same cultivation conditions, the content of glutamic acid in the dried leaves is 1.5 times or more compared to a control stevia plant,

[0092] (A1-11') when cultivated under the same cultivation conditions, the content of glycine in the dried leaves is 1.5 times or more compared to a control stevia plant,

[0093] (A1-12') when cultivated under the same cultivation conditions, the content of histidine in the dried leaves is 1.6 times or more compared to a control stevia plant,

[0094] (A1-13') when cultivated under the same cultivation conditions, the content of isoleucine in the dried leaves is 1.5 times or more compared to a control stevia plant,

[0095] (A1-14') when cultivated under the same cultivation conditions, the content of leucine in the dried leaves is 1.2 times or more compared to a control stevia plant,

[0096] (A1-15') when cultivated under the same cultivation conditions, the content of lysine in the dried leaves is 1.4 times or more compared to a control stevia plant,

[0097] (A1-16') when cultivated under the same cultivation conditions, the content of phenylalanine in the dried leaves is more than 1.0 times compared to a control stevia plant,

[0098] (A1-17') when cultivated under the same cultivation conditions, the content of proline in the dried leaves is 1.8 times or more compared to a control stevia plant,

[0099] (A1-18') when cultivated under the same cultivation conditions, the content of serine in the dried leaves is 3.7 times or more compared to a control stevia plant,

[0100] (A1-19') when cultivated under the same cultivation conditions, the content of threonine in the dried leaves is 1.5 times or more compared to a control stevia plant,

[0101] (A1-20') when cultivated under the same cultivation conditions, the content of tyrosine in the dried leaves is 1.7 times or more compared to a control stevia plant,

[0102] (A1-21') when cultivated under the same cultivation conditions, the content of valine in the dried leaves is 1.5 times or more compared to a control stevia plant,

[0103] (A1-22') when cultivated under the same cultivation conditions, the content of RebD in the dried leaves is 2.9 times or more compared to a control stevia plant,

[0104] (A1-23') when cultivated under the same cultivation conditions, the content of RebM in the dried leaves is 1.7 times or more compared to a control stevia plant,

[0105] (A1-24') when cultivated under the same cultivation conditions, the total content of RebD and RebM in the dried leaves is 2.4 times or more compared to a control stevia plant,

[0106] (A1-25') when cultivated under the same cultivation conditions, the weight ratio of RebD to TSG in the dried leaves is 3.8 times or more compared to a control stevia plant,

[0107] (A1-26') when cultivated under the same cultivation conditions, the weight ratio of RebM relative to TSG in the dried leaves is 2.1 times or more compared to the control stevia plant body,

[0108] (A1-27') when cultivated under the same cultivation conditions, the weight ratio of the total of RebD and RebM relative to TSG in the dried leaves is 3.1 times or more compared to the control stevia plant body.

[0109] In a particular manner, the content of iron in the dried leaves can be 3.0 ppb or more, 3.1 ppb or more, 3.2 ppb or more, 3.3 ppb or more, 3.4 ppb or more, 3.5 ppb or more, 3.6 ppb or more, 3.7 ppb or more, 3.8 ppb or more, 3.9 ppb or more, 4.0 ppb or more, 4.1 ppb or more, 4.2 ppb or more, 4.3 ppb or more, 4.4 ppb or more, 4.5 ppb or more, 4.6 ppb or more, 4.7 ppb or more, 4.8 ppb or more, 4.9 ppb or more, 5.0 ppb or more, 5.1 ppb or more, 5.2 ppb or more, 5.3 ppb or more, 5.4 ppb or more, 5.5 ppb or more, 5.6 ppb or more, 5.7 ppb or more, 5.8 ppb or more, 5.9 ppb or more, 6.0 ppb or more, 6.1 ppb or more, 6.2 ppb or more, 6.3 ppb or more, 6.4 ppb or more, 6.5 ppb or more, 6.6 ppb or more, 6.7 ppb or more, 6.8 ppb or more, 6.9 ppb or more, or 7.0 ppb or more (hereinafter, sometimes referred to as "chemical feature A2-1 of the present invention").

[0110] In a particular manner, the content of zinc in the dried leaves can be 55 ppb or more, 57 ppb or more, 60 ppb or more, 62 ppb or more, 65 ppb or more, 67 ppb or more, 70 ppb or more, 72 ppb or more, 75 ppb or more, 77 ppb or more, 80 ppb or more, 82 ppb or more, 85 ppb or more, 87 ppb or more, 90 ppb or more, 92 ppb or more, 95 ppb or more, 97 ppb or more, or 100 ppb or more (hereinafter, sometimes referred to as "chemical feature A2-2 of the present invention").

[0111] In a particular aspect, the content of phosphorus (inorganic phosphorus) in the dried leaf can be 13000 ppb or more, 13500 ppb or more, 14000 ppb or more, 14500 ppb or more, 15000 ppb or more, 15500 ppb or more, 16000 ppb or more, 16500 ppb or more, 17000 ppb or more, 17500 ppb or more, 18000 ppb or more, 18500 ppb or more, 19000 ppb or more, 19500 ppb or more, 20000 ppb or more, 20500 ppb or more, 21000 ppb or more, 21500 ppb or more, 22000 ppb or more, 22500 ppb or more, 23000 ppb or more, 23500 ppb or more, 24000 ppb or more, 24500 ppb or more, 25000 ppb or more, 25500 ppb or more, 26000 ppb or more, 26500 ppb or more, 27000 ppb or more, 27500 ppb or more, 28000 ppb or more, 28500 ppb or more, 29000 ppb or more, 29500 ppb or more, 30000 ppb or more, 30500 ppb or more, 31000 ppb or more, 31500 ppb or more, 32000 ppb or more, 32500 ppb or more, 33000 ppb or more, 33500 ppb or more, 34000 ppb or more, 34500 ppb or more, or 35000 ppb or more (hereinafter, sometimes referred to as "chemical feature A2-3 of the present application").

[0112] In a particular aspect, the content of copper in the dried leaf can be 35 ppb or more, 40 ppb or more, 45 ppb or more, 50 ppb or more, 55 ppb or more, 60 ppb or more, 65 ppb or more, 70 ppb or more, 75 ppb or more, 80 ppb or more, 85 ppb or more, 90 ppb or more, 95 ppb or more, 100 ppb or more, 105 ppb or more, 110 ppb or more, 115 ppb or more, 120 ppb or more, 125 ppb or more, 130 ppb or more, 135 ppb or more, 140 ppb or more, 145 ppb or more, or 150 ppb or more (hereinafter, sometimes referred to as "chemical feature A2-4 of the present application").

[0113] In a particular aspect, the content of methionine in the dried leaf of the plant body of the present application can be 0.30 ppm or more, 0.35 ppm or more, 0.40 ppm or more, 0.45 ppm or more, 0.50 ppm or more, 0.55 ppm or more, 0.60 ppm or more, 0.65 ppm or more, 0.70 ppm or more, 0.75 ppm or more, 0.80 ppm or more, 0.85 ppm or more, 0.90 ppm or more, 0.95 ppm or more, 1.00 ppm or more, 1.05 ppm or more, 1.10 ppm or more, 1.15 ppm or more, 1.20 ppm or more, 1.25 ppm or more, 1.30 ppm or more, 1.35 ppm or more, 1.40 ppm or more, 1.45 ppm or more, 1.50 ppm or more, 1.55 ppm or more, 1.60 ppm or more, 1.65 ppm or more, 1.70 ppm or more, 1.75 ppm or more, 1.80 ppm or more, 1.85 ppm or more, 1.90 ppm or more, 1.95 ppm or more, or 2.00 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-5 of the present application").

[0114] In a particular aspect, the content of methionine in the dried leaf of the plant body of the present application can be 0.30 ppm or more, 0.35 ppm or more, 0.40 ppm or more, 0.45 ppm or more, 0.50 ppm or more, 0.55 ppm or more, 0.60 ppm or more, 0.65 ppm or more, 0.70 ppm or more, 0.75 ppm or more, 0.80 ppm or more, 0.85 ppm or more, 0.90 ppm or more, 0.95 ppm or more, 1.00 ppm or more, 1.05 ppm or more, 1.10 ppm or more, 1.15 ppm or more, 1.20 ppm or more, 1.25 ppm or more, 1.30 ppm or more, 1.35 ppm or more, 1.40 ppm or more, 1.45 ppm or more, 1.50 ppm or more, 1.55 ppm or more, 1.60 ppm or more, 1.65 ppm or more, 1.70 ppm or more, 1.75 ppm or more, 1.80 ppm or more, 1.85 ppm or more, 1.90 ppm or more, 1.95 ppm or more, or 2.00 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-5 of the present application").

[0115] In a particular aspect, the content of arginine in the dried leaf of the plant body of the present application can be 1.0 ppm or more, 1.1 ppm or more, 1.2 ppm or more, 1.3 ppm or more, 1.4 ppm or more, 1.5 ppm or more, 1.6 ppm or more, 1.7 ppm or more, 1.8 ppm or more, 1.9 ppm or more, 2.0 ppm or more, 2.1 ppm or more, 2.2 ppm or more, 2.3 ppm or more, 2.4 ppm or more, 2.5 ppm or more, 2.6 ppm or more, 2.7 ppm or more, 2.8 ppm or more, 2.9 ppm or more, 3.0 ppm or more, 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, or 4.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-7 of the present application").

[0116] In a particular aspect, the content of arginine in the dried leaf of the plant body of the present application can be 1.0 ppm or more, 1.1 ppm or more, 1.2 ppm or more, 1.3 ppm or more, 1.4 ppm or more, 1.5 ppm or more, 1.6 ppm or more, 1.7 ppm or more, 1.8 ppm or more, 1.9 ppm or more, 2.0 ppm or more, 2.1 ppm or more, 2.2 ppm or more, 2.3 ppm or more, 2.4 ppm or more, 2.5 ppm or more, 2.6 ppm or more, 2.7 ppm or more, 2.8 ppm or more, 2.9 ppm or more, 3.0 ppm or more, 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, or 4.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-7 of the present application").

[0117] In a particular aspect, the content of aspartate in the dried leaf can be 0.5 ppm or more, 0.6 ppm or more, 0.7 ppm or more, 0.8 ppm or more, 0.9 ppm or more, 1.0 ppm or more, 1.1 ppm or more, 1.2 ppm or more, 1.3 ppm or more, 1.4 ppm or more, or 1.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-9 of the present application") in the case of the plant body of the present application.

[0118] In a particular aspect, the content of glutamate in the dried leaf can be 1.5 ppm or more, 1.6 ppm or more, 1.7 ppm or more, 1.8 ppm or more, 1.9 ppm or more, 2.0 ppm or more, 2.1 ppm or more, 2.2 ppm or more, 2.3 ppm or more, 2.4 ppm or more, 2.5 ppm or more, 2.6 ppm or more, 2.7 ppm or more, 2.8 ppm or more, 2.9 ppm or more, or 3.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-10 of the present application") in the case of the plant body of the present application.

[0119] In a particular aspect, the content of glycine in the dried leaf can be 0.4 ppm or more, 0.5 ppm or more, 0.6 ppm or more, 0.7 ppm or more, 0.8 ppm or more, 0.9 ppm or more, or 1.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-11 of the present application") in the case of the plant body of the present application.

[0120] In a particular aspect, the content of histidine in the dried leaf can be 0.9 ppm or more, 1.0 ppm or more, 1.1 ppm or more, 1.2 ppm or more, 1.3 ppm or more, 1.4 ppm or more, or 1.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-12 of the present application") in the case of the plant body of the present application.

[0121] In a particular aspect, the content of isoleucine in the dried leaf can be 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, 5.0 ppm or more, 5.1 ppm or more, 5.2 ppm or more, 5.3 ppm or more, 5.4 ppm or more, or 5.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-13 of the present application") in the case of the plant body of the present application. In a particular aspect, the content of isoleucine in the dried leaf can be 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, 5.0 ppm or more, 5.1 ppm or more, 5.2 ppm or more, 5.3 ppm or more, 5.4 ppm or more, or 5.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-13 of the present application") in the case of the plant body of the present application.

[0122] In a particular aspect, the content of leucine in the dried leaf of the plant body of the present application can be 7.6 ppm or more, 7.7 ppm or more, 7.8 ppm or more, 7.9 ppm or more, 8.0 ppm or more, 8.1 ppm or more, 8.2 ppm or more, 8.3 ppm or more, 8.4 ppm or more, 8.5 ppm or more, 8.6 ppm or more, 8.7 ppm or more, 8.8 ppm or more, 8.9 ppm or more, 9.0 ppm or more, 9.1 ppm or more, 9.2 ppm or more, 9.3 ppm or more, 9.4 ppm or more, 9.5 ppm or more, 9.6 ppm or more, 9.7 ppm or more, 9.8 ppm or more, 9.9 ppm or more, 10.0 ppm or more, 10.1 ppm or more, 10.2 ppm or more, 10.3 ppm or more, 10.4 ppm or more, 10.5 ppm or more, 10.6 ppm or more, 10.7 ppm or more, 10.8 ppm or more, 10.9 ppm or more, 11.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-14 of the present application").

[0123] In a particular aspect, the content of lysine in the dried leaf of the plant body of the present application can be 2.9 ppm or more, 3.0 ppm or more, 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, or 5.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-15 of the present application").

[0124] In a particular aspect, the content of phenylalanine in the dried leaf of the plant body of the present application can be 11.4 ppm or more, 11.6 ppm or more, 11.8 ppm or more, 12.0 ppm or more, 12.2 ppm or more, 12.4 ppm or more, 12.6 ppm or more, 12.8 ppm or more, 13.0 ppm or more, 13.2 ppm or more, 13.4 ppm or more, 13.6 ppm or more, 13.8 ppm or more, 14.0 ppm or more, 14.2 ppm or more, 14.4 ppm or more, 14.6 ppm or more, 14.8 ppm or more, 15.0 ppm or more, 15.2 ppm or more, 15.4 ppm or more, 15.6 ppm or more, 15.8 ppm or more, or 16.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-16 of the present application").

[0125] In a particular aspect, the content of proline in the dried leaf of the plant body of the present application can be 2.8 ppm or more, 2.9 ppm or more, 3.0 ppm or more, 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, 5.0 ppm or more, 5.1 ppm or more, 5.2 ppm or more, 5.3 ppm or more, 5.4 ppm or more, 5.5 ppm or more, 5.6 ppm or more, 5.7 ppm or more, 5.8 ppm or more, 5.9 ppm or more, or 6.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-17 of the present application").

[0126] In a particular aspect, the content of proline in the dried leaf of the plant body of the present application can be 2.8 ppm or more, 2.9 ppm or more, 3.0 ppm or more, 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, 3.5 ppm or more, 3.6 ppm or more, 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, 5.0 ppm or more, 5.1 ppm or more, 5.2 ppm or more, 5.3 ppm or more, 5.4 ppm or more, 5.5 ppm or more, 5.6 ppm or more, 5.7 ppm or more, 5.8 ppm or more, 5.9 ppm or more, or 6.0 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-17 of the present application").

[0127] In a particular aspect, the content of threonine in the dried leaf of the plant body of the present application can be 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, 5.0 ppm or more, 5.1 ppm or more, 5.2 ppm or more, 5.3 ppm or more, 5.4 ppm or more, or 5.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-19 of the present application").

[0128] In a particular aspect, the content of tyrosine in the dried leaf of the plant body of the present application can be 2.2 ppm or more, 2.3 ppm or more, 2.4 ppm or more, 2.5 ppm or more, 2.6 ppm or more, 2.7 ppm or more, 2.8 ppm or more, 2.9 ppm or more, 3.0 ppm or more, 3.1 ppm or more, 3.2 ppm or more, 3.3 ppm or more, 3.4 ppm or more, or 3.5 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-20 of the present application").

[0129] In a particular aspect, the content of valine in the dried leaf of the plant body of the present application can be 3.7 ppm or more, 3.8 ppm or more, 3.9 ppm or more, 4.0 ppm or more, 4.1 ppm or more, 4.2 ppm or more, 4.3 ppm or more, 4.4 ppm or more, 4.5 ppm or more, 4.6 ppm or more, 4.7 ppm or more, 4.8 ppm or more, 4.9 ppm or more, 5.0 ppm or more, 5.1 ppm or more, 5.2 ppm or more, 5.3 ppm or more, 5.4 ppm or more, 5.5 ppm or more, 5.6 ppm or more, 5.7 ppm or more, or 5.8 ppm or more (hereinafter, sometimes referred to as "chemical feature A2-21 of the present application").

[0130] In a particular manner, the content of RebD in the dried leaves can be 0.5% by weight or more, 0.6% by weight or more, 0.7% by weight or more, 0.8% by weight or more, 0.9% by weight or more, 1.0% by weight or more, 1.1% by weight or more, 1.2% by weight or more, 1.3% by weight or more, 1.4% by weight or more, 1.5% by weight or more, 1.6% by weight or more, 1.7% by weight or more, 1.8% by weight or more, 1.9% by weight or more, 2.0% by weight or more, 2.1% by weight or more, 2.2% by weight or more, 2.3% by weight or more, 2.4% by weight or more, 2.5% by weight or more, 2.6% by weight or more, 2.7% by weight or more, 2.8% by weight or more, 2.9% by weight or more, 3.0% by weight or more, 3.1% by weight or more, 3.2% by weight or more, 3.3% by weight or more, 3.4% by weight or more, or 3.5% by weight or more, in terms of the plant body of the present application (hereinafter, sometimes referred to as "Chemical feature A2-22 of the present application").

[0131] In a particular manner, the content of RebM in the dried leaves can be 0.27% by weight or more, 0.30% by weight or more, 0.35% by weight or more, 0.40% by weight or more, 0.45% by weight or more, 0.50% by weight or more, 0.55% by weight or more, 0.60% by weight or more, 0.65% by weight or more, 0.70% by weight or more, 0.75% by weight or more, 0.80% by weight or more, 0.85% by weight or more, 0.90% by weight or more, 0.95% by weight or more, 1.00% by weight or more, 1.05% by weight or more, 1.10% by weight or more, 1.15% by weight or more, 1.20% by weight or more, 1.25% by weight or more, 1.30% by weight or more, 1.35% by weight or more, 1.40% by weight or more, 1.45% by weight or more, or 1.50% by weight or more, in terms of the plant body of the present application (hereinafter, sometimes referred to as "Chemical feature A2-23 of the present application").

[0132] In a particular aspect, the total content of RebD and RebM in the dried leaves of the plant body of the present application can be 0.8% by weight or more, 0.9% by weight or more, 1.0% by weight or more, 1.1% by weight or more, 1.2% by weight or more, 1.3% by weight or more, 1.4% by weight or more, 1.5% by weight or more, 1.6% by weight or more, 1.7% by weight or more, 1.8% by weight or more, 1.9% by weight or more, 2.0% by weight or more, 2.1% by weight or more, 2.2% by weight or more, 2.3% by weight or more, 2.4% by weight or more, 2.5% by weight or more, 2.6% by weight or more, 2.7% by weight or more, 2.8% by weight or more, 2.9% by weight or more, 3.0% by weight or more, 3.1% by weight or more, 3.2% by weight or more, 3.3% by weight or more, 3.4% by weight or more, 3.5% by weight or more, 3.6% by weight or more, 3.7% by weight or more, 3.8% by weight or more, 3.9% by weight or more, or 4.0% by weight or more (hereinafter, sometimes referred to as "Chemical feature A2-24 of the present application").

[0133] In a particular aspect, the weight ratio of RebD to TSG (RebD / TSG) in the dried leaves of the plant body of the present application can be 5% or more, 6% or more, 7% or more, 8% or more, 9% or more, 10% or more, 11% or more, 12% or more, 13% or more, 14% or more, 15% or more, 16% or more, 17% or more, 18% or more, 19% or more, 20% or more, 21% or more, 22% or more, 23% or more, 24% or more, 25% or more, 26% or more, 27% or more, 28% or more, 29% or more, or 30% or more (hereinafter, sometimes referred to as "Chemical feature A2-25 of the present application").

[0134] In a particular aspect, the weight ratio of RebM to TSG (RebM / TSG) in the dried leaves of the plant body of the present application can be 1.0% or more, 1.5% or more, 2.0% or more, 2.5% or more, 3.0% or more, 3.5% or more, 4.0% or more, 4.5% or more, 5.0% or more, 5.5% or more, 6.0% or more, 6.5% or more, 7.0% or more, 7.5% or more, 8.0% or more, 8.5% or more, 9.0% or more, 9.5% or more, 10.0% or more, 10.5% or more, 11.0% or more, 11.5% or more, 12.0% or more, 12.5% or more, 13.0% or more, 13.5% or more, 14.0% or more, 14.5% or more, 15.0% or more, 15.5% or more, 16.0% or more, 16.5% or more, 17.0% or more, 17.5% or more, or 18.0% or more (hereinafter, sometimes referred to as "Chemical feature A2-26 of the present application").

[0135] In a specific manner, the plant body of the present application can have a weight ratio of the total of RebD and RebM in the dried leaves to TSG (RebDM / TSG) of 5.5% or more, 7.0% or more, 8.5% or more, 10.0% or more, 11.5% or more, 13.0% or more, 14.5% or more, 16.0% or more, 17.5% or more, 19.0% or more, 20.5% or more, 22.0% or more, 23.5% or more, 25.0% or more, 26.5% or more, 28.0% or more, 29.5% or more, 31.0% or more, 32.5% or more, 34.0% or more, 35.5% or more, 37.0% or more, 38.5% or more, or 40.0% or more (hereinafter, sometimes referred to as "Chemical feature A2-27 of the present application").

[0136] The plant body of the present application can also have two or more of the above-described features in combination. For example, the plant body of the present application can have at least two of the chemical features A2-1 to A2-27 of the present application (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty, twenty-one, twenty-two, twenty-three, twenty-four, twenty-five, twenty-six, or twenty-seven of the chemical features A2-1 to A2-27). In some aspects, the plant body of the present application has at least two of the following features (A2-1') to (A2-27') (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty, twenty-one, twenty-two, twenty-three, twenty-four, twenty-five, twenty-six, or twenty-seven of the features (A2-1') to (A2-27')):

[0137] (A2-1') the content of iron in the dried leaves is 3.0 ppb or more,

[0138] (A2-2') the content of zinc in the dried leaves is 55 ppb or more,

[0139] (A2-3') the content of phosphorus in the dried leaves is 13,000 ppb or more,

[0140] (A2-4') the content of copper in the dried leaves is 35 ppb or more,

[0141] (A2-5') the content of molybdenum in the dried leaves is 0.30 ppb or more,

[0142] (A2-6') the content of alanine in the dried leaves is 21.0 ppm or more,

[0143] (A2-7) the content of arginine in the dried leaves is 1.0 ppm or more,

[0144] (A2-8) the content of asparagine in the dried leaves is 2.5 ppm or more,

[0145] (A2-9) the content of aspartic acid in the dried leaves is 0.5 ppm or more,

[0146] (A2-10) the content of glutamic acid in the dried leaves is 1.5 ppm or more,

[0147] (A2-11) the content of glycine in the dried leaves is 0.4 ppm or more,

[0148] (A2-12) the content of histidine in the dried leaves is 0.9 ppm or more,

[0149] (A2-13) the content of isoleucine in the dried leaves is 3.1 ppm or more,

[0150] (A2-14) the content of leucine in the dried leaves is 7.6 ppm or more,

[0151] (A2-15) the content of lysine in the dried leaves is 2.9 ppm or more,

[0152] (A2-16) the content of phenylalanine in the dried leaves is 11.4 ppm or more,

[0153] (A2-17) the content of proline in the dried leaves is 2.8 ppm or more,

[0154] (A2-18) the content of serine in the dried leaves is 27 ppm or more,

[0155] (A2-19) the content of threonine in the dried leaves is 3.8 ppm or more,

[0156] (A2-20) the content of tyrosine in the dried leaves is 2.2 ppm or more,

[0157] (A2-21) the content of valine in the dried leaves is 3.7 ppm or more,

[0158] (A2-22) the content of RebD in the dried leaves is 1.3% by weight or more,

[0159] (A2-23) the content of RebM in the dried leaves is 0.43% by weight or more,

[0160] (A2-24) the combined content of RebD and RebM in the dried leaves is 0.8% by weight or more,

[0161] (A2-25') The weight ratio of RebD in the dried leaves to TSG is 18.8% or more,

[0162] (A2-26') The weight ratio of RebM in the dried leaves to TSG is 6.6% or more,

[0163] (A2-27') The weight ratio of the total of RebD and RebM in the dried leaves to TSG is 25.4% or more.

[0164] The content of the mineral can be determined by any known method such as ICP mass spectrometry, atomic absorption spectrometry, ICP emission spectrometry, etc. described in the Examples described later. The content of the amino acid can be determined by any known method such as HPLC, or pre-column derivatization method, post-column derivatization method, etc. described in the Examples described later. The content of the steviol glycoside such as RebD, RebM, etc. can be determined by the method described in Ohta et al., J. Appl. Glycosci., Vol. 57, No. 3, 199-209 (2010) or WO2010 / 038911, or the method described in the Examples described later. More specifically, the content of the steviol glycoside can be determined by, for example, sampling fresh leaves from the stevia plant body, and performing LC / MS-MS, etc.

[0165] In other aspects, the plant body of the present application has a low expression level of FRO2 compared to a control stevia plant body having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1 (hereinafter, sometimes referred to as "chemical feature B of the present application"). By "a low expression level of FRO2 compared to a control stevia plant body", it is meant, for example, that when cultivated under the same cultivation conditions, the expression level of FRO2 is lower than that of the control stevia plant body. More specifically, for example, when cultivated under the same cultivation conditions, the expression level (e.g., FPKM value) of the FRO2 gene in fresh leaves is 5.0% or more, 5.2% or more, 5.4% or more, 5.6% or more, 5.8% or more, 6.0% or more, 6.2% or more, 6.4% or more, 6.6% or more, 6.8% or more, 7.0% or more, 7.2% or more, 7.4% or more, 7.6% or more, 7.8% or more, 8.0% or more, 8.2% or more, 8.4% or more, 8.6% or more, 8.8% or more, 9.0% or more, 9.2% or more, 9.4% or more, 9.6% or more, 9.8% or more, or 10.0% or more lower than the expression level of the FRO2 gene in fresh leaves of the control stevia plant body. The expression level to be compared can be the median value of a plurality of individuals. In some aspects, for the plant body of the present application, when cultivated under the same cultivation conditions, the FPKM value of the expression level of the FRO2 gene in fresh leaves is 6.8% or more lower than the expression level of the FRO2 gene in fresh leaves of the control stevia plant body.

[0166] The amount of expression of FRO2 can be determined by the sequencing based on NGS and the like described in the Examples below or any known method for determining the amount of expression of a gene, for example, various hybridization methods using a nucleic acid molecule that specifically hybridizes to a nucleic acid molecule encoding FRO2 or a unique fragment thereof (for example, in situ hybridization), Northern blotting, Southern blotting, various PCR methods, immunoprecipitation using a substance that can specifically recognize FRO2 such as an antibody, EIA (enzyme immunoassay) (for example, ELISA (emzyme-linked immunosorbent assay) and the like), RIA (radio immuno assay) (for example, IRMA (immunoradiometric assay), RAST (radioallergosorbent test), RIST (radioimmunosorbent test) and the like), Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, MRI and the like. The genomic sequence, CDS sequence and amino acid sequence of steviol FRO2 are shown in SEQ ID NOs: 13, 14 and 15, respectively.

[0167] On the other hand, with respect to the plant body of the present application, the control stevia plant body having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1 contains less RebA (hereinafter, sometimes referred to as "chemical feature C1 of the present application"). "Contains less than the control stevia plant body" means, for example, that the content of RebA (for example, the content in dried leaves) is less than that of the control stevia plant body when cultivated under the same cultivation conditions. More specifically, for example, with respect to the plant body of the present application, the content of RebA in dried leaves can be 0.70-fold or less, 0.68-fold or less, 0.66-fold or less, 0.64-fold or less, 0.62-fold or less, 0.60-fold or less, 0.58-fold or less, 0.56-fold or less, 0.54-fold or less, 0.52-fold or less, 0.50-fold or less, 0.48-fold or less, 0.46-fold or less, 0.44-fold or less, 0.42-fold or less, 0.40-fold or less, 0.38-fold or less, 0.36-fold or less, 0.34-fold or less, 0.32-fold or less, 0.30-fold or less, 0.28-fold or less, 0.26-fold or less, 0.24-fold or less, 0.22-fold or less, 0.20-fold or less, 0.18-fold or less, 0.16-fold or less, 0.14-fold or less, 0.12-fold or less, or 0.10-fold or less, particularly 0.48-fold or less, than that in the control stevia plant body when cultivated under the same cultivation conditions. The contents compared can be the average of a plurality of individuals.

[0168] In a specific manner, the content of RebA in the dried leaves of the plant body of the present application can be 10.0% by weight or less, 9.6% by weight or less, 9.2% by weight or less, 8.8% by weight or less, 8.4% by weight or less, 8.0% by weight or less, 7.6% by weight or less, 7.2% by weight or less, 6.8% by weight or less, 6.4% by weight or less, 6.0% by weight or less, 5.6% by weight or less, 5.2% by weight or less, 4.8% by weight or less, 4.4% by weight or less, 4.0% by weight or less, 3.6% by weight or less, 3.2% by weight or less, 2.8% by weight or less, 2.4% by weight or less, or 2.0% by weight or less when cultivated under the same cultivation conditions (hereinafter, sometimes referred to as "chemical feature C2 of the present application").

[0169] The plant body of the present application can also have two or more of the above-described chemical features in combination. For example, the plant body of the present application can have two or three of the chemical features A to C of the present application. In a more specific manner, the plant body of the present application can also have at least two of the chemical features Al-1 to Al-27, A2-1 to A2-27, B, Cl, and C2 of the present application, for example, two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, fifteen, sixteen, seventeen, eighteen, nineteen, twenty, twenty-one, twenty-two, twenty-three, twenty-four, twenty-five, twenty-six, twenty-seven, twenty-eight, twenty-nine, thirty, thirty-one, thirty-two, thirty-three, thirty-four, thirty-five, thirty-six, thirty-seven, thirty-eight, thirty-nine, forty, forty-one, forty-two, forty-three, forty-four, forty-five, forty-six, forty-seven, forty-eight, forty-nine, fifty, fifty-one, fifty-two, fifty-three, fifty-four, fifty-five, fifty-six, fifty-seven, fifty-eight, or fifty-nine of these chemical features.

[0170] The plant body of the present application can include not only the whole plant body, but also a plant organ (for example, a leaf, a petal, a stem, a root, a seed, and the like), a plant tissue (for example, an epidermis, a phloem, a parenchyma, a xylem, a vascular bundle, a palisade tissue, a spongy tissue, and the like), or a plant cell in various forms (for example, a suspension culture cell), a protoplast, a section of a leaf, a callus, and the like. In addition, the leaf can be the dried leaf described above.

[0171] In addition, the plant body of the present application can also include a tissue culture or a plant culture cell. This is because the plant body can be regenerated by culturing such a tissue culture or a plant culture cell. As examples of the tissue culture or the plant culture cell of the plant body of the present application, an embryo, a meristem cell, pollen, a leaf, a root, a root tip, a petal, a protoplast, a section of a leaf, a callus, and the like can be listed, but are not limited thereto.

[0172] 2. Method for producing the plant body of the present application

[0173] The present application provides, on the other hand, a method for producing a stevia plant (hereinafter, sometimes referred to as "the production method of the present application"), which is a stevia plant having more of at least one nutrient selected from the group consisting of iron, zinc, phosphorus, copper, molybdenum, amino acids, RebD and RebM, and / or a lower expression amount of FRO2 than a control stevia plant having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1, characterized by comprising a step of crossing the stevia plant of the present application with a second stevia plant.

[0174] The stevia plant produced by the method can have the same genetic traits as the stevia plant of the present application.

[0175] The content of minerals, amino acids, RebD and RebM, or the range of the weight ratio of RebD and RebM to TSG, the range of the fold of the content or the weight ratio relative to that in the control stevia plant, the range of the fold of the expression amount of FRO2 relative to that in the control stevia plant, and the like, in the stevia plant obtained by the production method of the present application are as described above with respect to the stevia plant of the present application.

[0176] In the production method of the present application, the "crossing" means obtaining a child plant (a stevia plant produced by the production method of the present application (second generation (S2)) by mating the stevia plant of the present application (first generation (S1)) and the second stevia plant (S1). As the method of crossing, backcrossing is preferred. The "backcrossing" means a method of further crossing the child plant (S2) born between the stevia plant of the present application and the second stevia plant with the stevia plant of the present application (i.e., a stevia plant having the genetic traits of the present application) (S1) to produce a stevia plant having the genetic traits of the present application. The second stevia plant (S1) used in the production method of the present application is substantially backcrossed when it has the same genetic traits as the stevia plant of the present application.

[0177] Alternatively, the stevia plant of the present application can be produced by self-fertilization. The self-fertilization can be performed by self-pollination of the stevia plant of the present application by allowing pollen of the stamen of the stevia plant of the present application to reach the pistil of the stevia plant of the present application.

[0178] The stevia plant produced by the production method of the present application has the same genetic traits as the stevia plant of the present application, and thus a stevia plant having the same phenotype as the stevia plant of the present application can be produced by further crossing the stevia plant produced by the production method of the present application with a third stevia plant.

[0179] As other means, the plant body of the present application can also be produced by culturing the above-mentioned tissue culture or plant culture cell in a manner to regenerate a plant body. As for the culture conditions, the same as those of the tissue culture or plant culture cell of the wild-type stevia plant, are well known (Protocols for In Vitro cultures and secondary metabolite analysis of aromatic and medicinal plants, Method in molecular biology, vo. 1391, pp 113-123).

[0180] Further as other means, the plant body of the present application can be produced by introducing the variation of the present application into the genome of the stevia plant body. The introduction of the variation can be performed by a transgenic method, or by a non-transgenic method. As examples of the "non-transgenic method", there can be cited methods of inducing a genetic variation of a host cell (or host plant body) without introducing a foreign gene. As such methods, there can be cited methods of allowing a mutagen to act on a plant cell. As such mutagens, there can be cited ethyl methanesulfonate (EMS) and sodium azide, etc. For example, EMS can be treated on a plant cell at a concentration of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, etc. The treatment time is 1 to 48 hours, 2 to 36 hours, 3 to 30 hours, 4 to 28 hours, 5 to 26 hours, 6 to 24 hours. The treatment procedure itself is well known, and can be performed by immersing a water-absorbing seed subjected to a water-absorbing process in a treatment liquid containing a mutagen at the above-mentioned concentration for the above-mentioned treatment time.

[0181] Further, as an example of a non-transgenic method, a method of irradiating a plant cell with a radioactive ray such as X-ray, γ-ray, ultraviolet ray, or light ray can be exemplified. At this time, the cell after irradiation with an appropriate amount of ultraviolet ray (ultraviolet lamp intensity, distance, time) is cultured in a selection medium or the like, and a cell, callus, or plant body having a target trait can be selected. The irradiation intensity at this time is 0.01 to 100 Gr, 0.03 to 75 Gr, 0.05 to 50 Gr, 0.07 to 25 Gr, 0.09 to 20 Gr, 0.1 to 15 Gr, 0.1 to 10 Gr, 0.5 to 10 Gr, or 1 to 10 Gr, the irradiation distance is 1 cm to 200 m, 5 cm to 100 m, 7 cm to 75 m, 9 cm to 50 m, 10 cm to 30 m, 10 cm to 20 m, or 10 cm to 10 m, and the irradiation time is 1 minute to 2 years, 2 minutes to 1 year, 3 minutes to 0.5 years, 4 minutes to 1 month, 5 minutes to 2 weeks, or 10 minutes to 1 week. The intensity, distance, and time of irradiation differ depending on the kind of radioactive ray or the state of the object of irradiation (cell, callus, or plant body), and can be appropriately adjusted by those skilled in the art.

[0182] Further, methods such as cell fusion, anther culture (haploid culture), and distant hybridization (haploid culture) are also known.

[0183] Generally, a plant cell is sometimes accompanied by mutation during culture, and thus it is preferable to restore it to a plant individual in order to maintain a more stable trait.

[0184] A plant body obtained by performing transgenesis (for example, by genome editing or the like) after using the plant body of the present application as a host (for example, a plant body in which transgenesis is performed using the plant body of the present application as a host, and further, other traits are added) is also not excluded from the scope of the present application.

[0185] The plant body of the present application can be a plant body obtained by a transgenic method or its offspring (hereinafter, sometimes referred to as "transgenic plant body"), or a plant body obtained by a non-transgenic method or its offspring (hereinafter, sometimes referred to as "non-transgenic plant body").

[0186] 3. Screening method for plant body of the present application

[0187] The plant body of the present application and a plant body having the same genetic trait as the plant body of the present application can be screened by detecting the genetic trait of the present application from the tissue of a plant body to be examined. Here, the "screening" means to identify the plant body of the present application and a plant body other than the plant body of the present application, and to select the plant body of the present application.

[0188] Thus, the present application provides, on the other hand, a screening method for the stevia plant of the present application (hereinafter, sometimes referred to as "screening method A of the present application"), characterized by comprising a step of detecting whether or not the genotype at the position corresponding to position 37 of SEQ ID NO: 1 in the stevia plant to be tested is C / C (e.g., the presence and / or absence of the genetic trait of the present application).

[0189] The presence of the genetic trait of the present application can be determined by detecting the absence of an allele (e.g., an allele comprising the base sequence of SEQ ID NO: 16, 17, or 18) in which the base at the position corresponding to position 37 of SEQ ID NO: 1 is other than C (e.g., T), and optionally detecting the presence of an allele (e.g., an allele comprising the base sequence of SEQ ID NO: 4, 5, or 6) in which the base at the position corresponding to position 37 of SEQ ID NO: 1 is C.

[0190] The absence of the genetic trait of the present application can be determined by detecting the absence of an allele (e.g., an allele comprising the base sequence of SEQ ID NO: 5, 6, or 7) in which the base at the position corresponding to position 37 of SEQ ID NO: 1 is C, and / or detecting the presence of an allele (e.g., an allele comprising the base sequence of SEQ ID NO: 16, 17, or 18) in which the base at the position corresponding to position 37 of SEQ ID NO: 1 is other than C (e.g., T).

[0191] As a specific example of the method for detecting the genetic trait of the present application, there can be mentioned PCR, TaqMan PCR, sequencing, microarray, Invader, TILLING, RAD, RFLP, PCR-SSCP, AFLP, SSLP, CAPS, dCAPS, ASO, ARMS, DGGE, CCM, DOL, MALDI-TOF / MS, TDI, padlock probe, molecular beacon, DASH, UCAN, ECA, PINPOINT, PROBE, VSET, Survivor assay, Sniper assay, Luminex assay, GOOD, LCx, SNaPshot, Mass ARRAY, Pyrosequences, SNP-IT, melting curve analysis, and the like, but the present application is not limited thereto.

[0192] In one mode, the genetic trait of the present application can be detected by dCAPS using the following primer set and restriction enzyme.

[0193] • Primer set:

[0194] A primer set comprising a forward primer comprising the sequence of SEQ ID NO: 19 at the 3' end and any continuous sequence (e.g., a continuous sequence of any length) that continues to the 5' side from position 304 of SEQ ID NO: 13 optionally added to the 5' end of the sequence, and a reverse primer comprising a sequence complementary to any continuous sequence of 20 bases or more to the 3' side of position 39 of SEQ ID NO: 9 or 10 (e.g., SEQ ID NO: 8 or 20). The sequences of the primers can be optimized within the range that satisfies the above conditions. For optimization of primer design, refer to, for example, Sambrook and Russell et al. described above. In addition, each of the above primers can be 15 to 50 bases long, 18 to 48 bases long, 20 to 45 bases long, 30 to 65 bases long, etc.

[0195] • Restriction enzyme: Afl III

[0196] In a particular mode, the genetic trait of the present application can be detected by the dCAPS method using a forward primer composed of the sequence of SEQ ID NO: 7, a reverse primer composed of the sequence of SEQ ID NO: 8, and the restriction enzyme Afl III.

[0197] The screening method A of the present application can further comprise a step of evaluating the content of a nutritional component and / or the expression amount of FRO2 in the test stevia plant in which the genetic trait of the present application is detected. The evaluation of the content of a nutritional component and the expression amount of FRO2 is as described in the item of the plant body of the present application. In addition, in this mode, from the test stevia plant bodies in which the genetic trait of the present application is detected, an individual having a high content of a nutritional component and / or an individual having a low expression amount of FRO2 is selected, and is mated with other stevia plant bodies, and a child plant body is obtained, and the screening method A of the present application is also applicable to the child plant body. Thus, the screening method A of the present application can comprise one or more of the following steps.

[0198] (i) a step of detecting the genetic trait of the present application in the genome of a test stevia plant,

[0199] (ii) a step of evaluating the content of a nutritional component and / or the expression amount of FRO2 in the tissue of a test stevia plant in which the genetic trait of the present application is detected,

[0200] (iii) a step of selecting an individual having a high content of a nutritional component and / or an individual having a low expression amount of FRO2 from the test stevia plant bodies in which the genetic trait of the present application is detected,

[0201] (iv) a step of mating the selected individual with other stevia plant bodies,

[0202] (v) a step of detecting the genetic trait of the present application in the genome of a child plant body obtained by mating,

[0203] (vi) a step of evaluating the content of the nutrient component and / or the expression amount of FRO2 in the plant tissue of the progeny plant in which the genetic trait of the present application is detected,

[0204] (vii) a step of selecting a plant in which the content of the nutrient component is high and / or the expression amount of FRO2 is low from among the progeny plants of the plant in which the genetic trait of the present application is detected.

[0205] The plant in which the content of the nutrient component is high, for example, can be a plant in which the content of the nutrient component is as high as the top 50%, the top 40%, the top 30%, the top 25%, the top 20%, the top 15%, the top 10%, the top 5%, the top 4%, the top 3%, the top 2%, or the top 1% among the plants of the sweet gum in which the genetic trait of the present application is detected. The plant in which the expression amount of FRO2 is low, for example, can be a plant in which the expression amount of FRO2 is as low as the top 50%, the top 40%, the top 30%, the top 25%, the top 20%, the top 15%, the top 10%, the top 5%, the top 4%, the top 3%, the top 2%, or the top 1% among the plants of the sweet gum in which the genetic trait of the present application is detected. In addition, the other sweet gum plants to be crossed can or can not contain the genetic trait of the present application. In the above-described manner, the steps (iv) to (vii) can be repeated multiple times. In this way, a sweet gum plant in which the content of the nutrient component is higher and / or the expression amount of FRO2 is lower can be selected.

[0206] In the screening method A of the present application, the plant of the sweet gum to be detected can be a natural plant, a non-transgenic plant, or a transgenic plant. As for the non-transgenic plant, the method for producing the plant of the present application is described in the item.

[0207] In the screening method A of the present application, the plant of the sweet gum to be detected can also contain a sweet gum plant subjected to a sudden mutation induction treatment and its progeny plant. As for the sudden mutation induction treatment, the treatment based on a mutation inducer, the treatment based on irradiation of a radioactive ray or a light ray, and the like are described in the item of the plant of the present application.

[0208] In addition, the present application provides the primer set described above, for example, a primer set characterized by containing a forward primer containing the base sequence of SEQ ID NO: 7 or 19 and a reverse primer containing the base sequence of SEQ ID NO: 8 or 20. The present application further provides a primer set that can amplify the region having the base sequence of SEQ ID NO: 1 by PCR, for example, a primer set characterized by being a primer set of a forward primer containing the base sequence of SEQ ID NO: 2 and a reverse primer containing the base sequence of SEQ ID NO: 3.

[0209] Further, the present application provides a probe (hereinafter, sometimes referred to as "the probe of the present application") which can detect the presence and / or absence of the genetic trait of the present application. The probe of the present application can have a structure suitable for various methods of detecting the presence and / or absence of the genetic trait of the present application. For example, the probe of the present application can comprise a base sequence having complementarity to a portion of the genome comprising the variant site of the present application. As non-limiting examples of the probe, a probe comprising a base sequence selected from the group consisting of SEQ ID NOs: 4 to 6, 16 to 18 can be listed. Among these sequences, SEQ ID NOs: 4 to 6 are specific to the allele containing the variant of the present application, and SEQ ID NOs: 16 to 18 are specific to the allele not containing the variant of the present application. The presence of the genetic trait of the present application can be detected by detecting the allele containing the variant of the present application, and / or not detecting the allele not containing the variant of the present application, and the absence of the genetic trait of the present application can be detected by not detecting the allele containing the variant of the present application, and / or detecting the allele not containing the variant of the present application. The probe of the present application preferably has a label. As non-limiting examples of the label involved, a fluorescent label, a luminescent label, a radioactive label, a pigment, an enzyme, a quencher, a moiety which can bind to a detectable label, and the like can be listed. In a particular mode, the probe of the present application has a base sequence having complementarity to a base sequence selected from the group consisting of SEQ ID NOs: 4 to 6, 16 to 18, and a label.

[0210] The present application further provides a kit comprising the primer set described above and the restriction enzyme Afl III, for example, a screening kit, wherein the primer set comprises a forward primer comprising the sequence of SEQ ID NO: 19 at the 3' terminus and any continuous sequence (e.g., a continuous sequence of any length) continuing to the 5' side from the position 304 of SEQ ID NO: 13 optionally added to the 5' terminus of the sequence, and a reverse primer comprising a sequence complementary to any continuous sequence of 20 bases or more to the 3' side of the position 39 of SEQ ID NO: 9 or 10 (e.g., SEQ ID NO: 8 or 20), for example, a primer set comprising a forward primer consisting of the sequence of SEQ ID NO: 7 and a reverse primer consisting of the sequence of SEQ ID NO: 8.

[0211] Further, the present application provides a screening kit comprising a primer set which can amplify a region having a base sequence selected from the group consisting of SEQ ID NOs: 4 to 6, 16 to 18 by PCR, and the probe of the present application.

[0212] These primer sets, probes, and kits can be used for detecting the genetic trait of the present application, for the screening method A of the present application, and the like. In addition, in these primer sets and kits, an instruction in which an explanation related to the detection of the genetic trait of the present application or the screening method A of the present application is recorded, for example, a website information (e.g., URL, two-dimensional code) including information related to the instruction for use or the method for use, a medium including information related to the method for use, for example, a floppy disk, a CD, a DVD, a Blu-ray disc, a memory card, a USB memory, and the like can be included.

[0213] In one aspect, the present application provides a screening kit for the plant body of the present application, characterized by comprising a reagent for detecting whether the genotype at a position corresponding to position 37 of SEQ ID NO: 1 is C / C (e.g., the presence and / or absence of the genetic trait of the present application). The reagent can include a primer and / or a probe used in the CAPS method, the dCAPS method, or the TaqMan PCR method. In a specific aspect, the reagent for detecting the presence and / or absence of the genetic trait of the present application includes: a combination of a primer set and a restriction enzyme for detecting the genetic trait of the present application by the dCAPS method, such as a combination of a primer set including a forward primer consisting of the sequence of SEQ ID NO: 7 and a reverse primer consisting of the sequence of SEQ ID NO: 8 and the restriction enzyme AflIII, or a combination of a primer set for amplifying a site related to the genetic trait of the present application (e.g., a site including a sequence selected from the group consisting of SEQ ID NOs: 1, 4 to 6, and 16 to 18) and a probe having a base sequence complementary to a site related to the genetic trait of the present application (e.g., a site including a sequence selected from the group consisting of SEQ ID NOs: 4 to 6 and 16 to 18), which can be used in the TaqMan PCR method, and the like.

[0214] 4. Method for producing extract from plant body and product using the extract

[0215] In a further aspect of the present application, there is provided a method for producing an extract containing at least one kind of nutritional ingredient selected from the group consisting of a mineral (e.g., iron, zinc, phosphorus, copper, molybdenum, and the like), an amino acid (e.g., alanine, arginine, asparagine, aspartic acid, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, and the like), RebD, and RebM (hereinafter, sometimes referred to as "the method for producing an extract of the present application"), characterized by a step of obtaining an extract from the plant body of the present application or a seed or a leaf (e.g., dried leaf or fresh leaf) of the plant body. Further, there is provided a method for producing a nutritional ingredient preparation (hereinafter, sometimes referred to as "the method for producing a nutritional ingredient preparation of the present application"), characterized by a step of refining at least one kind of the above-described nutritional ingredient from the extract obtained by the method for producing an extract of the present application.

[0216] Specifically, provided is a method for producing a nutrient component concentrate, characterized by comprising a step of obtaining an extract containing the above-mentioned nutrient components from the plant body of the present application, the plant body of the present application selected by the screening method A of the present application, or the plant body of the present application produced by the method of the present application, and a step of concentrating the above-mentioned nutrient components from the obtained extract.

[0217] The extract containing the above-mentioned nutrient components can be obtained, for example, by reacting fresh leaves or dried leaves of the plant body of the present application with a suitable solvent (e.g., an aqueous solvent such as water or an organic solvent such as alcohol, ether, and acetone). For example, the extraction conditions of RebD or RebM, etc. can be referred to the methods described in Ohta et al. or WO2010 / 038911 described above.

[0218] Further, each of the nutrient components contained in the extract containing the above-mentioned nutrient components, such as RebD or RebM, can be concentrated by known methods such as a gradient of ethyl acetate and other organic solvents: water, HPLC, gas chromatography, TOF-MS, UPLC, etc.

[0219] One mode of the extract (hereinafter, referred to as "the extract of the present application") obtained by the method for producing an extract of the present application contains at least one of the above-mentioned nutrient components in a higher amount than a control stevia plant body having a genotype of C / T at a position corresponding to position 37 of SEQ ID NO: 1. The degree of the higher amount compared to the control stevia plant body is as described in the item of the plant body of the present application.

[0220] By mixing the extract of the present application thus obtained and / or the nutrient component concentrate obtained by the method for producing a nutrient component concentrate of the present application (e.g., minerals (e.g., iron, zinc, phosphorus, copper, molybdenum, etc.), amino acids (e.g., alanine, arginine, asparagine, aspartic acid, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, etc.), RebD and / or RebM) with other components, a pharmaceutical product, a flavor, or a food product containing the above-mentioned nutrient components can be produced. Thus, as another embodiment, the present application provides a method for producing a pharmaceutical product, a flavor, or a food product, characterized by comprising a step of mixing the extract of the present application and / or the nutrient component concentrate obtained by the method for producing a nutrient component concentrate of the present application with other components. Further, the present application provides a pharmaceutical product, a flavor, or a food product containing the above-mentioned nutrient components obtained by the production method. Here, the food product refers to a beverage and a food. Thus, in one embodiment, the present application provides a pharmaceutical product, a flavor, a beverage, or a food, and a method for producing the same.

[0221] Further, the present application provides a herbal tea product containing the dried product of the plant body of the present application. The part of the plant body contained in the herbal tea product of the present application is not particularly limited, and can be any part such as a leaf, a stem, a root, etc., and the dried product of a leaf, which contains more nutritional components, is preferable. The herbal tea product can be a pulverized product, a powder, or a granule of the dried product of the plant body (e.g., dried leaf), or a form in which the pulverized product, the powder, or the granule is filled into a tea bag or the like in a water-permeable package.

[0222] 5. Base sequence involved in the plant body of the present application

[0223] In another embodiment, the present application provides a base sequence involved in the plant body of the stevia plant of the present application. The base sequence involved in the plant body of the stevia plant of the present application contains or consists of a base sequence selected from the group consisting of SEQ ID NOs: 4 to 6, 21, and 22.

[0224] 6. Screening method for plant body rich in nutritional components

[0225] In another aspect, the present application provides a screening method for a plant body high in content of nutritional components (hereinafter, sometimes referred to as "screening method B of the present application"), characterized by comprising a step of measuring the expression amount of FRO2. According to the present application, it is clarified that the expression amount of FRO2 is inversely correlated with the content of nutritional components, and thus it is possible to screen a plant body high in content of nutritional components using the expression amount of FRO2 as an index.

[0226] In the screening method B of the present application, an individual low in expression amount of FRO2 can be selected. The expression amount of FRO2 low means, for example, that the expression amount of FRO2 is lower than the median value of the plant group to be examined. In another aspect, the expression amount of FRO2 low sometimes means that the expression amount of FRO2 is contained in the top 50%, 40%, 30%, 25%, 20%, 15%, 10%, 5%, 4%, 3%, 2%, or 1% of the plant group to be examined. In a particular aspect, the expression amount of FRO2 low sometimes means, for example, that the FPKM value of FRO2 analyzed in NGS-based sequencing is 3.2 or less, 3.1 or less, 3.0 or less, 2.9 or less, 2.8 or less, 2.7 or less, 2.6 or less, 2.5 or less, 2.4 or less, 2.3 or less, 2.2 or less, 2.1 or less, 2.0 or less, 1.9 or less, 1.8 or less, 1.7 or less, 1.6 or less, 1.5 or less, 1.4 or less, 1.3 or less, 1.2 or less, 1.1 or less, 1.0 or less, 0.9 or less, 0.8 or less, 0.7 or less, 0.6 or less, or 0.5 or less.

[0227] The determination of the expression level of FRO2 can be performed by any known method for determining the expression level of a gene, such as sequencing based on NGS or the like, various hybridization methods using a nucleic acid molecule that specifically hybridizes to a nucleic acid molecule encoding FRO2 or a unique fragment thereof (e.g., in situ hybridization), Northern blotting, Southern blotting, various PCR methods, immunoprecipitation using a substance that specifically recognizes FRO2 such as an antibody, EIA (e.g., ELISA or the like), RIA (e.g., IRMA, RAST, RIST or the like), Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, MRI or the like. The genomic sequence, CDS sequence and amino acid sequence of steviol FRO2 are shown in SEQ ID NOs: 13, 14 and 15, respectively. In addition, the sequences of FRO2 genes of other plants are known (e.g., Arabidopsis thaliana: NM_001331284 (base sequence: SEQ ID NO: 23, amino acid sequence: SEQ ID NO: 24), Phlegmariurus phlegmaria: XM_013589350 (base sequence: SEQ ID NO: 25, amino acid sequence: SEQ ID NO: 26), Helianthus annuus: XM_022180093 (base sequence: SEQ ID NO: 27, amino acid sequence: SEQ ID NO: 28), Manihot esculenta: XM_021755804 (base sequence: SEQ ID NO: 29, amino acid sequence: SEQ ID NO: 30)), and the sequences of FRO2 genes of plants other than these can be obtained by homology analysis of sequences obtained by sequencing or the like. In the determination of the expression level of FRO2, any plant tissue in which FRO2 can be expressed can be used, such as leaves, stems, roots, flowers or the like.

[0228] As the nutritional component, there is no particular limitation, and examples include minerals (e.g., iron, zinc, phosphorus, copper, molybdenum or the like), amino acids (e.g., alanine, arginine, asparagine, aspartic acid, cystine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine or the like), sweet components such as RebD and RebM, and the like.

[0229] The screening method B of the present application can further include a step of evaluating the content of a nutritional component in a test plant in which the expression level of FRO2 is low. The evaluation of the content of a nutritional component is as described in the item of the plant body of the present application. In addition, in this mode, from the test steviol plant body in which the genetic trait of the present application is detected, an individual having a high content of a nutritional component is selected, and is mated with another plant body, and a child plant body is obtained, and the screening method B of the present application is also applicable to this child plant body. Thus, the screening method B of the present application can include one or more of the following steps.

[0230] (i) a step of determining the expression level of FRO2 in a test plant,

[0231] (ii) a step of evaluating the content of the nutrient component in the plant tissue in which the expression amount of FRO2 is low,

[0232] (iii) a step of selecting an individual in which the content of the nutrient component is high from among the plant individuals in which the expression amount of FRO2 is low,

[0233] (iv) a step of crossing the selected individual with another plant individual,

[0234] (v) a step of measuring the expression amount of FRO2 in the child plant individual obtained by the crossing,

[0235] (vi) a step of evaluating the content of the nutrient component in the plant tissue in which the expression amount of FRO2 is low,

[0236] (vii) a step of selecting an individual in which the content of the nutrient component is high from among the child plant individuals in which the expression amount of FRO2 is low.

[0237] The individual in which the content of the nutrient component is high, which is selected, can be, for example, an individual in which the content of the nutrient component is as high as the top 50%, the top 40%, the top 30%, the top 25%, the top 20%, the top 15%, the top 10%, the top 5%, the top 4%, the top 3%, the top 2%, or the top 1% of the plant individuals in which the genetic trait of the present application is detected, and the like. Further, the expression amount of FRO2 in the other plant individual with which the crossing is performed can or can not be low. In the above-described manner, the steps (iv) to (vii) can be repeated a plurality of times. In this way, a plant individual in which the content of the nutrient component is higher can be screened.

[0238] Example

[0239] The following describes the examples and the like to which the present application relates, but the present application is not limited to these specific modes.

[0240] [Example 1] Correlation between RebD and RebM contents and genotype C / C

[0241] Based on commercially available stevia seeds, individual selection was performed based on growth condition, leaf shape, RebA, RebD, RebM content, and the like to obtain an individual group I. In the individual group I, genomic DNA was extracted from fresh leaves of each individual, and sequencing based on NGS (HiSeq 2500, Illumina) was performed. In addition, the content of steviol glycoside contained in the fresh leaves of each individual was quantified by LC-MS / MS (Shimadzu LCMS8050). Specifically, 0.25 g of fresh leaves was dried by freeze-drying, and 0.05 g of the dried and pulverized product was put into pure water. Extraction was performed by 20-minute ultrasonic treatment, and after centrifugation and filtration, 0.33 mL of an extract was obtained. LC-MS / MS analysis of the extract was performed by LCMS8050 ion mode (Shimadzu LCMS8050), and the concentration of steviol glycoside (RebA, RebB, RebC, RebD, RebE, RebF, RebG, RebM, RebN, and stevioside) was quantified. The average content of RebD, RebM, RebA, and TSG in the dried leaves of the individual of each genotype (% DW) and the proportion of RebD, RebM in the TSG content (%) is shown in Table 1, and the ratio of the average content of each steviol glycoside in the individual of genotype C / T or the proportion of the RebD, RebM content in the TSG content in the individual of genotype C / C is 1 is shown in Table 2, and the distribution of the RebD, RebM content in the dried leaves of the individual of each genotype (% DW) is shown in Figure 2 As shown in these results, the individual with high content of RebD and RebM is often genotype C / C. For example, the individual with a total content of RebD and RebM in the dried leaves of 1.3% by weight or more all had genotype C / C, and in 75% of the individuals with genotype C / C, the total content of RebD and RebM in the dried leaves was 1.3% by weight or more. On the other hand, in the individual with genotype C / T, no individual with a total content of RebD and RebM in the dried leaves of 1.3% by weight or more was confirmed. In addition, with respect to the RebA content and the TSG content, one of the individuals with genotype C / C had a tendency to be lower compared to the individual with genotype C / T. In addition, the TSG content means the total content of RebA, RebB, RebC, RebD, RebE, RebF, RebG, RebM, RebN, and stevioside, and RebDM means the total of RebD and RebM.

[0242] [Table 1]

[0243] Table 1 Average content of steviol glycoside in dried leaves of individual of each genotype

[0244]

[0245] The range in parentheses indicates the minimum value to the maximum value.

[0246] [Table 2]

[0247] Table 2 Fold of the average content of steviol glycosides in individuals of genotype C / C

[0248] RebD RebM RebDM RebA TSG RebD / TSG RebM / TSG RebDM / TSG 2.85 1.67 2.41 0.48 0.63 3.81 2.06 3.12

[0249] [Example 2] Correlation between FRO2 expression amount and genotype C / C

[0250] The SNP involved in the genotype C / C is located within the FRO2 gene. Total RNA was extracted from fresh leaves of each individual of the individual group I, and sequencing based on NGS (HiSeq 2500, Illumina) was performed. From the obtained data, the FPKM value of the FRO2 gene in each individual was calculated. As a result, the genotype C / C was more often seen in individuals in which the FRO2 gene expression amount was low (FPKM value) in dried leaves. Figure 3 For example, about 69% of individuals in which the FRO2 gene expression amount (FPKM value) in dried leaves was 2.5 or less had the genotype C / C, and in addition, 31% of individuals having the genotype C / C had the FRO2 gene expression amount (FPKM value) in dried leaves of 2.5 or less. In contrast, in individuals having the genotype C / T, individuals in which the FRO2 gene expression amount (FPKM value) in dried leaves was 2.5 or less were about 21%. From the above results, it was suggested that there was a tendency for the expression amount of the FRO2 gene to decrease by having the genotype C / C, as compared to individuals having the genotype C / T.

[0251] [Example 3] Correlation between mineral content and genotype C / C

[0252] With respect to representative 2 individuals of each genotype in the individual group I, the mineral components contained in the dried leaf extract were quantified. Fresh leaves were collected from each individual, and heat-dried at 65°C for about 17 hours. To each 100 mg of the obtained dried leaves, 1 mL of hot water (95°C or more) was added, and after mixing with a stirrer for 5 minutes, 10 μL of nitric acid was added to each 1 mL, and left overnight. The obtained sample was provided to ICP-MS (Agilent 7500cx, Agilent Technologies), and the concentration of each element was measured (using the attached MassHunter Workstation software). The average content of each mineral component in the dried leaves of individuals of each genotype is shown in Table 3, and the fold of the average content of each mineral component in individuals of the genotype C / C when the average content of each mineral component in individuals of the genotype C / T is 1 is shown in Tables 4 and 5, respectively. Figure 4Any one of the mineral components showed a content in the individuals of genotype C / C that was higher than that in the individuals of genotype C / T.

[0253] [Table 3]

[0254] Table 3 Average content of each mineral component in the dried leaves of individuals of each genotype (ppb)

[0255]

[0256] * The range in parentheses indicates the minimum to maximum value.

[0257] [Table 4]

[0258] Table 4 Ratio of average content of each mineral component in the individuals of genotype C / C

[0259] Fe P Cu Zn Mo 2.2 2.2 4.2 1.7 4.4

[0260] [Example 4] Correlation between amino acid content and genotype C / C

[0261] With respect to two representative individuals of each genotype in the individual group I, the free amino acids contained in the dried leaf extract were quantified. Fresh leaves were collected from each individual and heat-dried at 65°C for about 17 hours. To each 100 mg of the resulting dried leaves, 5 mL of hot water (95°C or higher) was added, and after mixing with a stirrer for 5 minutes, 10 minutes of ultrasonic treatment and 10 minutes of centrifugal treatment at 12,000 rpm were performed, and the supernatant was collected. The resulting supernatant sample was subjected to HPLC (Chromaster 5210 / 5260, Hitachi High-Tech) to measure the concentration of each amino acid (using Empower 3, Waters). The average content of each amino acid in the dried leaves of the individuals of each genotype is shown in Tables 5 to 6, and the ratio of the average content of each amino acid in the individuals of genotype C / C when the average content of each amino acid in the individuals of genotype C / T is 1 is shown in Tables 7 and 8, respectively. Figure 5 Any one of the amino acids showed a content in the individuals of genotype C / C that was higher than that in the individuals of genotype C / T.

[0262] [Table 5]

[0263] Table 5 Average content of each amino acid in the dried leaves of individuals of each genotype (ppm)

[0264]

[0265] * The range in parentheses indicates the minimum to maximum value.

[0266] [Table 6]

[0267] Table 6 Average content of each amino acid in dried leaves of individuals of each genotype 2 (ppm)

[0268]

[0269] The range in parentheses indicates the minimum to maximum value.

[0270] [Table 7]

[0271] Table 7 Ratio of average content of each amino acid in individuals of genotype C / C

[0272] Ser Ala Asn Arg Asp Glu Thr His Pro Lys Tyr Val Ile Leu Phe Gly 3.7 1.2 3.1 3.8 2.2 1.5 1.5 1.6 1.8 1.4 1.7 1.5 1.5 1.2 1.0 1.5

[0273] [Example 5] Correlation between RebD and RebM content and FRO2 expression amount

[0274] When the total content of RebD and RebM of each individual of population I obtained in Examples 1-2 was plotted against the FRO2 expression amount, it was confirmed that the two were inversely correlated Figure 6 ). In addition, the average values of the contents of RebD and RebM in the top / bottom 50%, 25%, 15%, and 10% of the individuals in terms of FRO2 expression amount are shown in Table 8.

[0275] [Table 8]

[0276] Table 8 Relationship between FRO2 expression amount and RebD and RebM content

[0277] FRO2 expression RebD (% DW) RebM (% DW) RebDM (% DW) Top 50% 0.71 0.30 1.01 Bottom 50% 1.15 0.43 1.58 Top 25% 0.69 0.32 1.01 Bottom 25% 1.41 0.51 1.92 Top 15% 0.49 0.29 0.79 Bottom 15% 1.41 0.60 2.00 Top 10% 0.49 0.24 0.74 Bottom 10% 1.42 0.64 2.07

[0278] As shown by these results, the average values of the contents of RebD and RebM were significantly lower in the group of individuals with low FRO2 expression amount than in the group of individuals with high FRO2 expression amount. The results of comparison of the total contents of RebD and RebM in the population of individuals ranked bottom 15% in terms of FRO2 expression amount and the population of individuals ranked top 15% in terms of FRO2 expression amount are shown in Figure 7 . Further, it was clarified that there was a stronger correlation between the ratio of the total content of RebD and RebM to the TSG content (RebDM / TSG) and the FRO2 expression amount Figure 8 ). SEQUENCE LISTING <110> MORI HOLDINGS CO., LTD. <120> Nutrient-rich stevia plant <130> G2738WO <150> JP 2021-069151 <151> 2021-04-15 <160> 30 <170> PatentIn version 3.5 <210> 1 <211> 70 <212> DNA <213> Stevia <400> 1 ataaacttta ggcccgacga tgttactcta cacattyccc gtcttgttca tggccgtagt 60 gggaagtgta 70 <210> 2 <211> 23 <212> DNA <213> Stevia <400> 2 ataaacttta ggcccgacga tgt 23 <210> 3 <211> 20 <212> DNA <213> Stevia <400> 3 tacacttccc actacggcca 20 <210> 4 <211> 21 <212> DNA <213> Stevia <400> 4 tctacacatt ccccgtcttg t 21 <210> 5 <211> 41 <212> DNA <213> Stevia <400> 5 acgatgttac tctacacatt ccccgtcttg ttcatggccg t 41 <210> 6 <211> 61 <212> DNA <213> Stevia <400> 6 tttaggcccg acgatgttac tctacacatt ccccgtcttg ttcatggccg tagtgggaag 60 t 61 <210> 7 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 7 gtataaactt taggcccgac gatgttactc tacacatg 38 <210> 8 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 8 gagaaaggaa agatatattt aaccatcatg 30 <210> 9 <211> 220 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 9 gtataaactt taggcccgac gatgttactc tacacatgcc ccgtcttgtt catggccgta 60 gtgggaagtg tatatctcca tttaagaaaa aaggcgaatc aaaaccgaga tgaagggtat 120 atatatatat aatagttttt tacttatttc agtaaattaa ataactgttt atttttactt 180 tatactattt catgatggtt aaatatatct ttcctttctc 220 <210> 10 <211> 220 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 10 gtataaactt taggcccgac gatgttactc tacacatgtc ccgtcttgtt catggccgta 60 gtgggaagtg tatatctcca tttaagaaaa aaggcgaatc aaaaccgaga tgaagggtat 120 atatatatat aatagttttt tacttatttc agtaaattaa ataactgttt atttttactt 180 tatactattt catgatggtt aaatatatct ttcctttctc 220 <210> 11 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 11 gtataaactt taggcccgac gatgttactc taca 34 <210> 12 <211> 186 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 12 catgtcccgt cttgttcatg gccgtagtgg gaagtgtata tctccattta agaaaaaagg 60 cgaatcaaaa ccgagatgaa gggtatatat atatataata gttttttact tatttcagta 120 aattaaataa ctgtttattt ttactttata ctatttcatg atggttaaat atatctttcc 180 tttctc 186 <210> 13 <211> 3232 <212> DNA <213> stevia <400> 13 atgattatga agatgatcaa agggtttgca attttgttgt ttataggcta ccttttccta 60 tgggtgatca caccaaccta tgtattcaag cagaagtggc tcgtgacggt tcgagcccac 120 accacttcta cttactttgg aactcaaggt ttgttccacc tctccaatca acatttttta 180 aatcactctg atttactagt ctaattacat cataatatat acaaaacata aaataacaga 240 tttaatagtt ttgttttcta actattttac gttttctttt aaatgtgtat aaactttagg 300 cccgacgatg ttactctaca cattycccgt cttgttcatg gccgtagtgg gaagtgtata 360 tctccattta agaaaaaagg cgaatcaaaa ccgagatgaa gggtatatat atatataata 420 gttttttact tatttcagta aattaaataa ctgtttattt ttactttata ctatttcatg 480 atggttaaat atatctttcc tttctcaaat tttagagaaa aagaagctac acccgataac 540 ttgagtatat ggagaagacc aatgatcatc aaaggtctcg ggatcgtctc gaaaatcgag 600 ctcgctttct ttgttatgtt tatcgctctt atcgtctggt ctttcacgac atacttacac 660 gtcggttttg ccaaaatcaa tcataaaaca gcggcaaaaa agggagagga agtgtaagtg 720 aatcaatatt ttaaatattt tgcaaatact acattcgtat attattatta acccgcgatc 780 gattctttg gttgatagtt gggaatcaaa gcttgattcg gtggctctac ggttcggatt 840 aattggcaac ttgtgtctag catttctgtt cttcccggtg actcgaggat cgtctatatt 900 gtcgttgttc gggcttactt ctgaggcgag tatcaagtat cacatatggc tcggtcatat 960 tgtatgact ctattcactt ctcatggtgt ttgttacatt atttattgga tcgttaccaa 1020 acagacacat gaggtacctt aatttttata ttattattta tgaattataa attagttaat 1080 attatggct cacaattcct gaaaatattc tgccagtgaa tttggaaaac tgaagataaa 1140 gtccataaat gttagaagat tttccacttt gaattaaagt caacttatat aatggtccca 1200 aatcatcgaa cattgtaaaa attaactcac caatgtgaaa aaccgaagat aggatcatat 1260 cccataactt tattctttgg tgatgaaatg acgaagctaa cataatttat ttaacagtcc 1320 ttttaaaaa tttctgatag atgttagacg taattaatta gttaattaat tattactcac 1380 cgaacaatga caaatgctta taaaagttgt ttttgaacta tcgttgttga tccgttgatg 1440 tcttttttca tatatgatat ccaattttcc tagtagtgta ttaaataatg ctatatgttt 1500 ttttagatgt tgaaatggga aaaaatcggc atatcaaatg tagccggaga gttggctttg 1560 gtagccggat tgattatgtg ggcaacaacg tttccaagga ttagacgaaa gatgtttgaa 1620 gttttcttct acgcacatca cttatacatc caatttatcg tgttcttcgt gttccatgtt 1680 ggcatagcat atgcatccat catgctaccg ggattctacc tcttcatgat tgatcgaatc 1740 ttgagatttt tacaatcaaa gggaaacgtt cgtgtggttt ctgctcgtgt tcttccgtgt 1800 gaatctctcg agctcaactt ctccaaaagc caaggtacgt aaaatagagc acaa a aagta 1860 tagaaaatat aaaggatcaa tttcttttta agtgataatg gtcgttaaca tttgtctttt 1920 aattttaagt cttttagatg tcacaatttt cattttttta tataggtcta caatatacac 1980 caacaagcat catattcatc aatgtcccaa gcatttcaaa ggctcaatgg catccattca 2040 ccattacttc tagtagcaat ttggagcccg aaaagctaag tgttatgatc aaaagtgaag 2100 gaagttggtc taggaaactt taccaaatgt tatcatcccc aaattctatt gaccggcttg 2160 atgtatgcat tgaaggacct tatgggccta tttcaaccaa ctttctaagg tattatgttc 2220 attacaatga cttgtgttag gttcatttca ttttattata cttaaaatga tcaaattatg 2280 gtttcaggca cgatatgcta gtgatggtga gcggaggaag tgggataaca ccatttatct 2340 caatatttag ggagcttgtt tacactaccg aaaccctaaa acgtaaagct ccaaaaattc 2400 tcttgataag ttcgtttaaa gactcttctg atctcaccat gctagaattg ttattaccat 2460 catctggtgc acctatagag ttttcaaaac tagagttaca aatcgaggca tatgtgacaa 2520 gagaaaacca gcccgttaat gataagaaac tagtgactgc cttatggttt aaaccaaatc 2580 cctctgacgc acccatcact cctattttag gacaaaatgg ttggttatgg cttggtgcga 2640 ttattgcatg ttctttgtg attttccttg tatctatggg gcttgtaaca cggttttaca 2700 tctatcctat tgataataac accaacaagg tgtattcata tggatcgagg gccgtgttaa 2760 acatgttgct catttgtgtg tctataatgg taacatgttg tttcggattt tggtggaata 2820 aaaagagaaa cgctatggac tcaaagcaaa ttcaaagcat ggagggtgtg acacccgtta 2880 gctcacccaa ctcatggttt tataacgcgg atagagagct tgaaagtgtt ccccaacaat 2940 cgctctttca gtctacaaat gttcattttg gcaaaagacc ggatcttaag agtaagacca 3000 gattctatgt ctaatgctac aaaagttaca cacttttgaa tatatgttaa tacaaaccct 3060 aacactttct attttgttta tatttttgtt aggaattttg tttgagcaaa aagaatcaag 3120 tgttggagtt cttgtttgtg ggccaaaaaa gatgaggcat gaggctgcaa atatatgttc 3180 atccggattg gcttcaaact tgcattttga gtccataagt tttagttggt ga 3232 <210> 14 <211> 2097 <212> DNA <213> Stevia rebaudiana <220> <221> CDS <222> (1)..(2097) <400> 14 atg aag atg atc aaa ggg ttt gca att ttg ttg ttt ata ggc tac ctt 48 Met Lys Met Ile Lys Gly Phe Ala Ile Leu Leu Phe Ile Gly Tyr Leu 1 5 10 15 ttc cta tgg gtg atc aca cca acc tat gta ttc aag cag aag tgg ctc 96 Phe Leu Trp Val Ile Thr Pro Thr Tyr Val Phe Lys Gln Lys Trp Leu 20 25 30 gtg acg gtt cga gcc cac acc act tct act tac ttt gga act caa ggt 144 Val Thr Val Arg Ala His Thr Thr Ser Thr Tyr Phe Gly Thr Gln Gly 35 40 45 ggc ccg acg atg tta ctc tac aca tty ccc gtc ttg ttc atg gcc gta 192 Gly Pro Thr Met Leu Leu Tyr Thr Phe Pro Val Leu Phe Met Ala Val 50 55 60 gtg gga agt gta tat ctc cat tta aga aaa aag gcg aat caa aac cga 240 Val Gly Ser Val Tyr Leu His Leu Arg Lys Lys Ala Asn Gln Asn Arg 65 70 75 80 gat gaa ggg aat ttt aga gaa aaa gaa gct aca ccc gat aac ttg agt 288 Asp Glu Gly Asn Phe Arg Glu Lys Glu Ala Thr Pro Asp Asn Leu Ser 85 90 95 ata tgg aga aga cca atg atc atc aaa ggt ctc ggg atc gtc tcg aaa 336 Ile Trp Arg Arg Pro Met Ile Ile Lys Gly Leu Gly Ile Val Ser Lys 100 105 110 atc gag ctc gct ttc ttt gtt atg ttt atc gct ctt atc gtc tgg tct 384 Ile Glu Leu Ala Phe Phe Val Met Phe Ile Ala Leu Ile Val Trp Ser 115 120 125 ttc acg aca tac tta cac gtc ggt ttt gcc aaa atc aat cat aaa aca 432 Phe Thr Thr Tyr Leu His Val Gly Phe Ala Lys Ile Asn His Lys Thr 130 135 140 gcg gca aaa aag gga gag gaa gtg tgg gaa tca aag ctt gat tcg gtg 480 Ala Ala Lys Lys Gly Glu Glu Val Trp Glu Ser Lys Leu Asp Ser Val 145 150 155 160 gct cta cgg ttc gga tta att ggc aac ttg tgt cta gca ttt ctg ttc 528 Ala Leu Arg Phe Gly Leu Ile Gly Asn Leu Cys Leu Ala Phe Leu Phe 165 170 175 ttc ccg gtg act cga gga tcg tct ata ttg tcg ttg ttc ggg ctt act 576 Phe Pro Val Thr Arg Gly Ser Ser Ile Leu Ser Leu Phe Gly Leu Thr 180 185 190 tct gag gcg agt atc aag tat cac ata tgg ctc ggt cat att gtt atg 624 Ser Glu Ala Ser Ile Lys Tyr His Ile Trp Leu Gly His Ile Val Met 195 200 205 act cta ttc act tct cat ggt gtt tgt tac att att tat tgg atc gtt 672 Thr Leu Phe Thr Ser His Gly Val Cys Tyr Ile Ile Tyr Trp Ile Val 210 215 220 acc aaa cag aca cat gag gta atg ttg aaa tgg gaa aaa atc ggc ata 720 Thr Lys Gln Thr His Glu Val Met Leu Lys Trp Glu Lys Ile Gly Ile 225 230 235 240 tca aat gta gcc gga gag ttg gct ttg gta gcc gga ttg att atg tgg 768 Ser Asn Val Ala Gly Glu Leu Ala Leu Val Ala Gly Leu Ile Met Trp 245 250 255 gca aca acg ttt cca agg att aga cga aag atg ttt gaa gtt ttc ttc 816 Ala Thr Thr Phe Pro Arg Ile Arg Arg Lys Met Phe Glu Val Phe Phe 260 265 270 tac gca cat cac tta tac atc caa ttt atc gtg ttc ttc gtg ttc cat 864 Tyr Ala His His Leu Tyr Ile Gin Phe Ile Val Phe Phe Val Phe His 275 280 285 gtt ggc ata gca tat gca tcc atc atg cta ccg gga ttc tac ctc ttc 912 Val Gly Ile Ala Tyr Ala Ser Ile Met Leu Pro Gly Phe Tyr Leu Phe 290 295 300 atg att gat cga atc ttg aga ttt tta caa tca aag gga aac gtt cgt 960 Met Ile Asp Arg Ile Leu Arg Phe Leu Gin Ser Lys Gly Asn Val Arg 305 310 315 320 gtg gtt tct gct cgt gtt ctt ccg tgt gaa tct ctc gag ctc aac ttc 1008 Val Val Ser Ala Arg Val Leu Pro Cys Glu Ser Leu Glu Leu Asn Phe 325 330 335 tcc aaa agc caa ggt ggt cta caa tat aca cca aca agc atc ata ttc 1056 Ser Lys Ser Gin Gly Gly Leu Gin Tyr Thr Pro Thr Ser Ile Ile Phe 340 345 350 atc aat gtc cca agc att tca aag gct caa tgg cat cca ttc acc att 1104 Ile Asn Val Pro Ser Ile Ser Lys Ala Gin Trp His Pro Phe Thr Ile 355 360 365 act tct agt agc aat ttg gag ccc gaa aag cta agt gtt atg atc aaa 1152 Thr Ser Ser Ser Asn Leu Glu Pro Glu Lys Leu Ser Val Met Ile Lys 370 375 380 agt gaa gga agt tgg tct agg aaa ctt tac caa atg tta tca tcc cca 1200 Ser Glu Gly Ser Trp Ser Arg Lys Leu Tyr Gln Met Leu Ser Ser Pro 385 390 395 400 aat tct att gac cgg ctt gat gta tgc att gaa gga cct tat ggg cct 1248 Asn Ser Ile Asp Arg Leu Asp Val Cys Ile Glu Gly Pro Tyr Gly Pro 405 410 415 att tca acc aac ttt cta agg cac gat atg cta gtg atg gtg agc gga 1296 Ile Ser Thr Asn Phe Leu Arg His Asp Met Leu Val Met Val Ser Gly 420 425 430 gga agt ggg ata aca cca ttt atc tca ata ttt agg gag ctt gtt tac 1344 Gly Ser Gly Ile Thr Pro Phe Ile Ser Ile Phe Arg Glu Leu Val Tyr 435 440 445 act acc gaa acc cta aaa cgt aaa gct cca aaa att ctc ttg ata agt 1392 Thr Thr Glu Thr Leu Lys Arg Lys Ala Pro Lys Ile Leu Leu Ile Ser 450 455 460 tcg ttt aaa gac tct tct gat ctc acc atg cta gaa ttg tta tta cca 1440 Ser Phe Lys Asp Ser Ser Asp Leu Thr Met Leu Glu Leu Leu Leu Pro 465 470 475 480 tca tct ggt gca cct ata gag ttt tca aaa cta gag tta caa atc gag 1488 Ser Ser Gly Ala Pro Ile Glu Phe Ser Lys Leu Glu Leu Gln Ile Glu 485 490 495 gca tat gtg aca aga gaa aac cag ccc gtt aat gat aag aaa cta gtg 1536 Ala Tyr Val Thr Arg Glu Asn Gln Pro Val Asn Asp Lys Lys Leu Val 500 505 510 act gcc tta tgg ttt aaa cca aat ccc tct gac gca ccc atc act cct 1584 Thr Ala Leu Trp Phe Lys Pro Asn Pro Ser Asp Ala Pro Ile Thr Pro 515 520 525 att tta gga caa aat ggt tgg tta tgg ctt ggt gcg att att gca tgt 1632 Ile Leu Gly Gln Asn Gly Trp Leu Trp Leu Gly Ala Ile Ile Ala Cys 530 535 540 tct ttt gtg att ttc ctt gta tct atg ggg ctt gta aca cgg ttt tac 1680 Ser Phe Val Ile Phe Leu Val Ser Met Gly Leu Val Thr Arg Phe Tyr 545 550 555 560 atc tat cct att gat aat aac acc aac aag gtg tat tca tat gga tcg 1728 Ile Tyr Pro Ile Asp Asn Asn Thr Asn Lys Val Tyr Ser Tyr Gly Ser 565 570 575 agg gcc gtg tta aac atg ttg ctc att tgt gtg tct ata atg gta aca 1776 Arg Ala Val Leu Asn Met Leu Leu Ile Cys Val Ser Ile Met Val Thr 580 585 590 tgt tgt ttc gga ttt tgg tgg aat aaa aag aga aac gct atg gac tca 1824 Cys Cys Phe Gly Phe Trp Trp Asn Lys Lys Arg Asn Ala Met Asp Ser 595 600 605 aag caa att caa agc atg gag ggt gtg aca ccc gtt agc tca ccc aac 1872 Lys Gln Ile Gln Ser Met Glu Gly Val Thr Pro Val Ser Ser Pro Asn 610 615 620 tca tgg ttt tat aac gcg gat aga gag ctt gaa agt gtt ccc caa caa 1920 Ser Trp Phe Tyr Asn Ala Asp Arg Glu Leu Glu Ser Val Pro Gln Gln 625 630 635 640 tcg ctc ttt cag tct aca aat gtt cat ttt ggc aaa aga ccg gat ctt 1968 Ser Leu Phe Gln Ser Thr Asn Val His Phe Gly Lys Arg Pro Asp Leu 645 650 655 aag gag caa aaa gaa tca agt gtt gga gtt ctt gtt tgt ggg cca aaa 2016 Lys Glu Gln Lys Glu Ser Ser Val Gly Val Leu Val Cys Gly Pro Lys 660 665 670 aag atg agg cat gag gct gca aat ata tgt tca tcc gga ttg gct tca 2064 Lys Met Arg His Glu Ala Ala Asn Ile Cys Ser Ser Gly Leu Ala Ser 675 680 685 aac ttg cat ttt gag tcc ata agt ttt agt tgg 2097[[ID=Z3]] Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 690 695 <210> 15 <211> 699 <212> PRT <213> Stevia <400> 15 Met Lys Met Ile Lys Gly Phe Ala Ile Leu Leu Phe Ile Gly Tyr Leu 1 5 10 15 Phe Leu Trp Val lie Thr Pro Thr Tyr Val Phe Lys Gin Lys Trp Leu 20 25 30 Val Thr Val Arg Ala His Thr Thr Ser Thr Tyr Phe Gly Thr Gin Gly 35 40 45 Gly Pro Thr Met Leu Leu Tyr Thr Phe Pro Val Leu Phe Met Ala Val 50 55 60 Val Gly Ser Val Tyr Leu His Leu Arg Lys Lys Ala Asn Gin Asn Arg 65 70 75 80 Asp Glu Gly Asn Phe Arg Glu Lys Glu Ala Thr Pro Asp Asn Leu Ser 85 90 95 Ile Trp Arg Arg Pro Met lie lie Lys Gly Leu Gly lie Val Ser Lys 100 105 110 Ile Glu Leu Ala Phe Phe Val Met Phe lie Ala Leu lie Val Trp Ser 115 120 125 Phe Thr Thr Tyr Leu His Val Gly Phe Ala Lys lie Asn His Lys Thr 130 135 140 Ala Ala Lys Lys Gly Glu Glu Val Trp Glu Ser Lys Leu Asp Ser Val 145 150 155 160 Ala Leu Arg Phe Gly Leu lie Gly Asn Leu Cys Leu Ala Phe Leu Phe 165 170 175 Phe Pro Val Thr Arg Gly Ser Ser lie Leu Ser Leu Phe Gly Leu Thr 180 185 190 Ser Glu Ala Ser lie Lys Tyr His lie Trp Leu Gly His lie Val Met 195 200 205 Thr Leu Phe Thr Ser His Gly Val Cys Tyr lie lie Tyr Trp lie Val 210 215 220 Thr Lys Gin Thr His Glu Val Met Leu Lys Trp Glu Lys lie Gly lie 225 230 235 240 Ser Asn Val Ala Gly Glu Leu Ala Leu Val Ala Gly Leu lie Met Trp 245 250 255 Ala Thr Thr Phe Pro Arg lie Arg Arg Lys Met Phe Glu Val Phe Phe 260 265 270 Tyr Ala His His Leu Tyr lie Gin Phe lie Val Phe Phe Val Phe His 275 280 285 Val Gly lie Ala Tyr Ala Ser lie Met Leu Pro Gly Phe Tyr Leu Phe 290 295 300 Met lie Asp Arg lie Leu Arg Phe Leu Gin Ser Lys Gly Asn Val Arg 305 310 315 320 Val Val Ser Ala Arg Val Leu Pro Cys Glu Ser Leu Glu Leu Asn Phe 325 330 335 Ser Lys Ser Gin Gly Gly Leu Gin Tyr Thr Pro Thr Ser He He Phe 340 345 350 He Asn Val Pro Ser He Ser Lys Ala Gin Trp His Pro Phe Thr He 355 360 365 Thr Ser Ser Ser Asn Leu Glu Pro Glu Lys Leu Ser Val Met He Lys 370 375 380 Ser Glu Gly Ser Trp Ser Arg Lys Leu Tyr Gin Met Leu Ser Ser Pro 385 390 395 400 Asn Ser He Asp Arg Leu Asp Val Cys He Gin Gly Pro Tyr Gly Pro 405 410 415 He Ser Thr Asn Phe Leu Arg His Asp Met Leu Val Met Val Ser Gly 420 425 430 Gly Ser Gly He Thr Pro Phe He Ser He Phe Arg Gin Leu Val Tyr 435 440 445 Thr Thr Gin Thr Leu Lys Arg Lys Ala Pro Lys He Leu Leu He Ser 450 455 460 Ser Phe Lys Asp Ser Ser Asp Leu Thr Met Leu Glu Leu Leu Leu Pro 465 470 475 480 Ser Ser Gly Ala Pro He Gin Phe Ser Lys Leu Glu Leu Gin He Gin 485 490 495 Ala Tyr Val Thr Arg Glu Asn Gln Pro Val Asn Asp Lys Lys Leu Val 500 505 510 Thr Ala Leu Trp Phe Lys Pro Asn Pro Ser Asp Ala Pro Ile Thr Pro 515 520 525 Ile Leu Gly Gln Asn Gly Trp Leu Trp Leu Gly Ala Ile Ile Ala Cys 530 535 540 Ser Phe Val Ile Phe Leu Val Ser Met Gly Leu Val Thr Arg Phe Tyr 545 550 555 560 Ile Tyr Pro Ile Asp Asn Asn Thr Asn Lys Val Tyr Ser Tyr Gly Ser 565 570 575 Arg Ala Val Leu Asn Met Leu Leu Ile Cys Val Ser Ile Met Val Thr 580 585 590 Cys Cys Phe Gly Phe Trp Trp Asn Lys Lys Arg Asn Ala Met Asp Ser 595 600 605 Lys Gln Ile Gln Ser Met Glu Gly Val Thr Pro Val Ser Ser Pro Asn 610 615 620 Ser Trp Phe Tyr Asn Ala Asp Arg Glu Leu Glu Ser Val Pro Gln Gln 625 630 635 640 Ser Leu Phe Gln Ser Thr Asn Val His Phe Gly Lys Arg Pro Asp Leu 645 650 655 Lys Glu Gln Lys Glu Ser Ser Val Gly Val Leu Val Cys Gly Pro Lys 660 665 670 Lys Met Arg His Glu Ala Ala Asn Ile Cys Ser Ser Gly Leu Ala Ser 675 680 685 Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 690 695 <210> 16 <211> 21 <212> DNA <213> Stevia rebaudiana <400> 16 tctacacatt tcccgtcttg t 21 <210> 17 <211> 41 <212> DNA <213> Stevia rebaudiana <400> 17 acgatgttac tctacacatt tcccgtcttg ttcatggccg t 41 <210> 18 <211> 61 <212> DNA <213> Stevia rebaudiana <400> 18 tttaggcccg acgatgttac tctacacatt tcccgtcttg ttcatggccg tagtgggaag 60 t 61 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 19 acgatgttac tctacacatg 20 <210> 20 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Synthetic DNA <400> 20 agatatattt aaccatcatg 20 <210> 21 <211> 70 <212> DNA <213> Stevia rebaudiana <400> 21 ataaacttta ggcccgacga tgttactcta cacattcccc gtcttgttca tggccgtagt 60 gggaagtgta 70 <210> 22 <211> 3232 <212> DNA <213> Stevia rebaudiana <400> 22 atgattatga agatgatcaa agggtttgca attttgttgt ttataggcta ccttttccta 60 tgggtgatca caccaaccta tgtattcaag cagaagtggc tcgtgacggt tcgagcccac 120 accacttcta cttactttgg aactcaaggt ttgttccacc tctccaatca acatttttta 180 aatcactctg atttactagt ctaattacat cataatatat acaaaacata aaataacaga 240 tttaatagtt ttgttttcta actattttac gttttctttt aaatgtgtat aaactttagg 300 cccgacgatg ttactctaca cattccccgt cttgttcatg gccgtagtgg gaagtgtata 360 tctccattta agaaaaaagg cgaatcaaaa ccgagatgaa gggtatatat atatataata 420 gttttttact tatttcagta aattaaataa ctgtttattt ttactttata ctatttcatg 480 atggttaaat atatctttcc tttctcaaat tttagagaaa aagaagctac acccgataac 540 ttgagtatat ggagaagacc aatgatcatc aaaggtctcg ggatcgtctc gaaaatcgag 600 ctcgctttct ttgttatgtt tatcgctctt atcgtctggt ctttcacgac atacttacac 660 gtcggttttg ccaaaatcaa tcataaaaca gcggcaaaaa agggagagga agtgtaagtg 720 aatcaatatt ttaaatattt tgcaaatact acattcgtat attattatta acccgcgatc 780 gattcttttg gttgatagtt gggaatcaaa gcttgattcg gtggctctac ggttcggatt 840 aattggcaac ttgtgtctag catttctgtt cttcccggtg actcgaggat cgtctatatt 900 gtcgttgttc gggcttactt ctgaggcgag tatcaagtat cacatatggc tcggtcatat 960 tgttatgact ctattcactt ctcatggtgt ttgttacatt atttattgga tcgttaccaa 1020 acagacacat gaggtacctt aatttttata ttattattta tgaattataa attagttaat 1080 atttatggct cacaattcct gaaaatattc tgccagtgaa tttggaaaac tgaagataaa 1140 gtccataaat gttagaagat tttccacttt gaattaaagt caacttatat aatggtccca 1200 aatcatcgaa cattgtaaaa attaactcac caatgtgaaa aaccgaagat aggatcatat 1260 cccataactt tattctttgg tgatgaaatg acgaagctaa cataatttat ttaacagtcc 1320 cttttaaaaa tttctgatag atgttagacg taattaatta gttaattaat tattactcac 1380 cgaacaatga caaatgctta taaaagttgt ttttgaacta tcgttgttga tccgttgatg 1440 tcttttttca tatatgatat ccaattttcc tagtagtgta ttaaataatg ctatatgttt 1500 ttttagatgt tgaaatggga aaaaatcggc atatcaaatg tagccggaga gttggctttg 1560 gtagccggat tgattatgtg ggcaacaacg tttccaagga ttagacgaaa gatgtttgaa 1620 gttttcttct acgcacatca cttatacatc caatttatcg tgttcttcgt gttccatgtt 1680 ggcatagcat atgcatccat catgctaccg ggattctacc tcttcatgat tgatcgaatc 1740 ttgagatttt tacaatcaaa gggaaacgtt cgtgtggttt ctgctcgtgt tcttccgtgt 1800 gatctctcg agctcaactt ctccaaagc caagtacgt aaatagagc aaaaagta 1860 Tagaaaatat aaaggatcaa ttcttttta agtgataatg gtcgttaaca ttgtctttt 1920 aattttaagt cttttagatg tcacaattt catttttta tataggtcta caatacac 1980 cacaagcat catattcattc atgtcccaa gcattcaatcaa ggctcaatgg catccattca 2040 ccattacttc tagtagcaat ttggagccg aaagctaag tgttatgatc aaagtgaag 2100 gaagttggtc taggaactt taccaatgt tatcatcccc aaattctt gaccggctg 2160 atgtatgcat tgaaggacct tatgggccta ttcaaccaa ctttctagg tattatgttc 2220 attack cttgtgttag gttcattca ttttatta cttaaaatga tcaattatg 2280 gtttcaggca cgatatgcta gtgatggtga gcggaggaag tgggataaca ccatttatct 2340 caatattag ggagcttgtt tacactaccg aaaccctaa acgtaagct ccaaaaattc 2400 tcttgataag ttcgtttaaa gactctctg atctcaccat gctagaattg ttattaccat 2460 catctggtgc acctatagag tttcaaac tagagttaca atcgaggca tatgtgacaa 2520 gagaaaacca gcccgttaat gataagaaac tagtgactgc cttatggttt aaaccaaatc 2580 cctctgacgc acccatcact cctattttag gacaaaatgg ttggttatgg cttggtgcga 2640 ttattgcatg ttcttttgtg attttcctlg tatctatggg gcttgtaaca cggttttaca 2700 tctatcctat tgataataac accaacaagg tgtattcata tggatcgagg gccgtgttaa 2760 acatgttgct catttgtgtg tctataatgg taacatgttg tttcggattt tggtggaata 2820 aaaagagaaa cgctatggac tcaaagcaaa ttcaaagcat ggagggtgtg acacccgtta 2880 gctcacccaa ctcatggttt tataacgcgg atagagagct tgaaagtgtt ccccaacaat 2940 cgctctttca gtctacaaat gttcattttg gcaaaagacc ggatcttaag agtaagacca 3000 gattctatgt ctaatgctac aaaagttaca cacttttgaa tatatgttaa tacaaaccct 3060 aacactttct attttgttta tatttttgtt aggaattttg tttgagcaaa aagaatcaag 3120 tgttggagtt cttgtttgtg ggccaaaaaa gatgaggcat gaggctgcaa atatatgttc 3180 atccggattg gcttcaaact tgcattttga gtccataagt tttagttggt ga 3232 <210> 23 <211> 2850 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (167)..(2578) <400> 23 aaatattgtt accataaatt cataaacttt cgttaaactt aaagtgattt atacacatag 60 tatattcaaa tatcgataaa tgtattttgc taatcaaatc gtatattctt ttataccagt 120 tttttaacgt catctcgcca aaacaacaac aaatagatgt aaaata atg aaa gaa 175 Met Lys Glu 1 aac aaa aaa caa gat cca tgg gga aga gtc aca tgc atg gag cat gca 223 Asn Lys Lys Gln Asp Pro Trp Gly Arg Val Thr Cys Met Glu His Ala 5 10 15 act agc tta ctt cat cca att ccc act caa aat tct ctc ttt ata tat 271 Thr Ser Leu Leu His Pro Ile Pro Thr Gln Asn Ser Leu Phe Ile Tyr 20 25 30 35 aca ctt tct cta cca cta ctt ccc tca aaa caa gct aac tcg atc ctt 319 Thr Leu Ser Leu Pro Leu Leu Pro Ser Lys Gln Ala Asn Ser Ile Leu 40 45 50 ctc act aaa gcg atc gta ccg atc tta ata tct tta gcg agc gag aga 367 Leu Thr Lys Ala Ile Val Pro Ile Leu Ile Ser Leu Ala Ser Glu Arg 55 60 65 gag aga gat aga aat cct gag agg aaa gag agg atg gag atc gaa aaa 415 Glu Arg Asp Arg Asn Pro Glu Arg Lys Glu Arg Met Glu Ile Glu Lys 70 75 80 agc aat aac ggt ggt tcg aac cca tct gca gga gaa gaa ttt aag gat 463 Ser Asn Asn Gly Gly Ser Asn Pro Ser Ala Gly Glu Glu Phe Lys Asp 85 90 95 atg att aag gga gtg act aag ttc ttg atg atg gtg att ttc ctt gga 511 Met Ile Lys Gly Val Thr Lys Phe Leu Met Met Val Ile Phe Leu Gly 100 105 110 115 acc atc atg ctt tgg atc atg atg cca aca cta acc tat cga acc aag 559 Thr Ile Met Leu Trp Ile Met Met Pro Thr Leu Thr Tyr Arg Thr Lys 120 125 130 tgg ttg cca cat ctg cgt atc aag ttt gga aca tcc act tat ttt ggt 607 Trp Leu Pro His Leu Arg Ile Lys Phe Gly Thr Ser Thr Tyr Phe Gly 135 140 145 gcc aca ggg acg acg ctt ttc atg tat atg ttc cca atg atg gtc gta 655 Ala Thr Gly Thr Thr Leu Phe Met Tyr Met Phe Pro Met Met Val Val 150 155 160 gct tgt ttg gga tgt gtc tat ctt cac ttc aag aat cga aag tcg cca 703 Ala Cys Leu Gly Cys Val Tyr Leu His Phe Lys Asn Arg Lys Ser Pro 165 170 175 cac cat atc gat agg gag acg aag gga gga gtg tgg agt aaa cta aga 751 His His Ile Asp Arg Glu Thr Lys Gly Gly Val Trp Ser Lys Leu Arg 180 185 190 195 aag cca atg tta gtg aag ggg cca tta gga ata gtc tca gtg act gag 799 Lys Pro Met Leu Val Lys Gly Pro Leu Gly Ile Val Ser Val Thr Glu 200 205 210 atc acc ttc ttg gcg atg ttt gtg gct ctt ctt ctc tgg tgc ttt atc 847 Ile Thr Phe Leu Ala Met Phe Val Ala Leu Leu Leu Trp Cys Phe Ile 215 220 225 act tat ttg cgc aat agc ttc gcc act atc act cct aaa tca gct gcc 895 Thr Tyr Leu Arg Asn Ser Phe Ala Thr Ile Thr Pro Lys Ser Ala Ala 230 235 240 gca cat gac gaa tct ttg tgg caa gcg aag ctg gaa tca gcg gcg cta 943 Ala His Asp Glu Ser Leu Trp Gln Ala Lys Leu Glu Ser Ala Ala Leu 245 250 255 agg tta gga ctg atc gga aac ata tgt ttg gcg ttc ttg ttc tta ccg 991 Arg Leu Gly Leu Ile Gly Asn Ile Cys Leu Ala Phe Leu Phe Leu Pro 260 265 270 275 gtg gct cgt ggg tca tcg ttg cta ccg gca atg gga tta acc tcg gag 1039 Val Ala Arg Gly Ser Ser Leu Leu Pro Ala Met Gly Leu Thr Ser Glu 280 285 290 tca agc atc aaa tac cat ata tgg ctc ggc cac atg gtc atg gcc ctt 1087 Ser Ser Ile Lys Tyr His Ile Trp Leu Gly His Met Val Met Ala Leu 295 300 305 ttc acc gtt cat ggt ctt tgt tac ata att tac tgg gcc tca atg cat 1135 Phe Thr Val His Gly Leu Cys Tyr Ile Ile Tyr Trp Ala Ser Met His 310 315 320 gaa atc tcc cag atg atc atg tgg gat acg aaa ggt gtc tca aat ttg 1183 Glu Ile Ser Gln Met Ile Met Trp Asp Thr Lys Gly Val Ser Asn Leu 325 330 335 gcc gga gag ata gct ctg gcg gcc gga ctt gtg atg tgg gcc acc aca 1231 Ala Gly Glu Ile Ala Leu Ala Ala Gly Leu Val Met Trp Ala Thr Thr 340 345 350 355 tat ccg aag ata agg aga aga ttc ttc gaa gtc ttc ttc tac act cac 1279 Tyr Pro Lys Ile Arg Arg Arg Phe Phe Glu Val Phe Phe Tyr Thr His 360 365 370 tat ctc tac atc gtc ttc atg ctc ttc ttt gtc ctc cac gtc ggc ata 1327 Tyr Leu Tyr Ile Val Phe Met Leu Phe Phe Val Leu His Val Gly Ile 375 380 385 agt ttc tcc ttc atc gct ctc cct ggt ttc tac atc ttc tta gtc gat 1375 Ser Phe Ser Phe Ile Ala Leu Pro Gly Phe Tyr Ile Phe Leu Val Asp 390 395 400 cgt ttc ctt cgg ttt ctt cag tca cgt gaa aat gtt cgc ttg ctc gct 1423 Arg Phe Leu Arg Phe Leu Gin Ser Arg Glu Asn Val Arg Leu Leu Ala 405 410 415 gct cgg att ctt cct tcc gac act atg gag ctc act ttc tcc aaa aac 1471 Ala Arg Ile Leu Pro Ser Asp Thr Met Glu Leu Thr Phe Ser Lys Asn 420 425 430 435 tct aag ttg gtt tat agc ccg acg agc ata atg ttt gtg aac ata ccg 1519 Ser Lys Leu Val Tyr Ser Pro Thr Ser Ile Met Phe Val Asn Ile Pro 440 445 450 agc att tca aag ctg caa tgg cat ccg ttt acg ata act tcc agt agc 1567 Ser Ile Ser Lys Leu Gln Trp His Pro Phe Thr Ile Thr Ser Ser Ser 455 460 465 aaa ctc gaa cca gag aag cta agt att gtt atc aag aaa gaa ggc aaa 1615 Lys Leu Glu Pro Glu Lys Leu Ser Ile Val Ile Lys Lys Glu Gly Lys 470 475 480 tgg tca act aag ctt cac cag agg ctt tct tct tct gat cag atc gac 1663 Trp Ser Thr Lys Leu His Gin Arg Leu Ser Ser Ser Asp Gin Ile Asp 485 490 495 cga ctt gct gtt tct gta gaa ggt cct tac ggc cct gct tcc gcc gat 1711 Arg Leu Ala Val Ser Val Glu Gly Pro Tyr Gly Pro Ala Ser Ala Asp 500 505 510 515 ttc tta agg cat gaa gct ttg gtt atg gtg tgc gga gga agc ggg ata 1759 Phe Leu Arg His Glu Ala Leu Val Met Val Cys Gly Gly Ser Gly Ile 520 525 530 act ccg ttt atc tct gtt att cgt gat ttg atc gcc aca agc caa aag 1807 Thr Pro Phe Ile Ser Val Ile Arg Asp Leu Ile Ala Thr Ser Gln Lys 535 540 545 gaa aca tgc aaa atc cca aaa ata act ctc att tgt gcg ttt aag aag 1855 Glu Thr Cys Lys Ile Pro Lys Ile Thr Leu Ile Cys Ala Phe Lys Lys 550 555 560 tct tca gag atc tcc atg ctc gat ctt gtc ttg cca tta tcc ggt ctt 1903 Ser Ser Glu Ile Ser Met Leu Asp Leu Val Leu Pro Leu Ser Gly Leu 565 570 575 gag act gaa cta tcc tcc gac att aac atc aaa atc gaa gcc ttc ata 1951 Glu Thr Glu Leu Ser Ser Asp Ile Asn Ile Lys Ile Glu Ala Phe Ile 580 585 590 595 acc cga gat aat gat gca gga gat gaa gca aaa gct ggg aaa atc aaa 1999 Thr Arg Asp Asn Asp Ala Gly Asp Glu Ala Lys Ala Gly Lys Ile Lys 600 605 610 act ctc tgg ttc aaa cca agt ctt tca gac caa tcc atc tca tca atc 2047 Thr Leu Trp Phe Lys Pro Ser Leu Ser Asp Gln Ser Ile Ser Ser Ile 615 620 625 ctc gga cca aac tca tgg ctt tgg ctc ggt gca att ctc gca tct tcg 2095 Leu Gly Pro Asn Ser Trp Leu Trp Leu Gly Ala Ile Leu Ala Ser Ser 630 635 640 ttc ttg atc ttc atg ata att att ggt atc atc act agg tat tac att 2143 Phe Leu Ile Phe Met Ile Ile Ile Gly Ile Ile Thr Arg Tyr Tyr Ile 645 650 655 tac ccg atc gac cac aac acc aac aag atc tat tct ttg act tca aaa 2191 Tyr Pro Ile Asp His Asn Thr Asn Lys Ile Tyr Ser Leu Thr Ser Lys 660 665 670 675 acc atc ata tat atc ttg gtc atc tcc gtg agc ata atg gcg act tgt 2239 Thr Ile Ile Tyr Ile Leu Val Ile Ser Val Ser Ile Met Ala Thr Cys 680 685 690 agt gcg gct atg ttg tgg aac aag aag aag tat ggt aaa gtt gaa agt 2287 Ser Ala Ala Met Leu Trp Asn Lys Lys Lys Tyr Gly Lys Val Glu Ser 695 700 705 aaa cag gtc caa aac gtc gac cgt cca tct cca aca tct tct cct acc 2335 Lys Gln Val Gln Asn Val Asp Arg Pro Ser Pro Thr Ser Ser Pro Thr 710 715 720 tca tca tgg ggt tac aat tcc ttg aga gaa atc gaa agt acg cca caa 2383 Ser Ser Trp Gly Tyr Asn Ser Leu Arg Glu Ile Glu Ser Thr Pro Gln 725 730 735 gaa tcg ctc gtg caa cgc acc aat ctg cat ttt gga gaa aga cct aat 2431 Glu Ser Leu Val Gln Arg Thr Asn Leu His Phe Gly Glu Arg Pro Asn 740 745 750 755 ctc aag aaa ctt cta ctt gat gtg gaa ggt tcg agt gtg gga gtt ctt 2479 Leu Lys Lys Leu Leu Leu Asp Val Glu Gly Ser Ser Val Gly Val Leu 760 765 770 gtt tgt ggt cct aag aag atg aga caa aag gtt gcg gaa ata tgt tcc 2527 Val Cys Gly Pro Lys Lys Met Arg Gln Lys Val Ala Glu Ile Cys Ser 775 780 785 tct ggt ttg gct gag aat ctt cat ttt gaa tct atc agt ttc agc tgg 2575 Ser Gly Leu Ala Glu Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 790 795 800 tga ttgcgtatat aactctgttt tcactatgtg ttgtgcaatt tgcttgagtt 2628 gcaagttaat aaggtggccc tgtgatttga cctatctttg tgtgctttac attgactttt 2688 cctgtttttt gtttttcttt atcctcttct aggaatattc agtgttaaaa aaaaaacttc 2748 ctttcaaaag ttccggaata tccttttttt gtttgtaaca caaggcatac agcttttgta 2808 aattgtaaat gttgaaatat aatactcatg ttgggtcata ta 2850 <210> 24 <211> 803 <212> PRT <213> Arabidopsis thaliana <400> 24 Met Lys Glu Asn Lys Lys Gin Asp Pro Trp Gly Arg Val Thr Cy s Met 1 5 10 15 Glu His Ala Thr Ser Leu Leu His Pro Ile Pro Thr Gin Asn Ser Leu 20 25 30 Phe Ile Tyr Thr Leu Ser Leu Pro Leu Leu Pro Ser Lys Gin Ala Asn 35 40 45 Ser Ile Leu Leu Thr Lys Ala Ile Val Pro Ile Leu Ile Ser Leu Ala 50 55 60 Ser Glu Arg Glu Arg Asp Arg Asn Pro Glu Arg Lys Glu Arg Met Glu 65 70 75 80 Ile Glu Lys Ser Asn Asn Gly Gly Ser Asn Pro Ser Ala Gly Glu Glu 85 90 95 Phe Lys Asp Met Ile Lys Gly Val Thr Lys Phe Leu Met Met Val Ile 100 105 110 Phe Leu Gly Thr Ile Met Leu Trp Ile Met Met Pro Thr Leu Thr Tyr 115 120 125 Arg Thr Lys Trp Leu Pro His Leu Arg Ile Lys Phe Gly Thr Ser Thr 130 135 140 Tyr Phe Gly Ala Thr Gly Thr Thr Leu Phe Met Tyr Met Phe Pro Met 145 150 155 160 Met Val Val Ala Cys Leu Gly Cys Val Tyr Leu His Phe Lys Asn Arg 165 170 175 Lys Ser Pro His His Ile Asp Arg Glu Thr Lys Gly Gly Val Trp Ser 180 185 190 Lys Leu Arg Lys Pro Met Leu Val Lys Gly Pro Leu Gly Ile Val Ser 195 200 205 Val Thr Glu Ile Thr Phe Leu Ala Met Phe Val Ala Leu Leu Leu Trp 210 215 220 Cys Phe Ile Thr Tyr Leu Arg Asn Ser Phe Ala Thr Ile Thr Pro Lys 225 230 235 240 Ser Ala Ala Ala His Asp Glu Ser Leu Trp Gln Ala Lys Leu Glu Ser 245 250 255 Ala Ala Leu Arg Leu Gly Leu Ile Gly Asn Ile Cys Leu Ala Phe Leu 260 265 270 Phe Leu Pro Val Ala Arg Gly Ser Ser Leu Leu Pro Ala Met Gly Leu 275 280 285 Thr Ser Glu Ser Ser Ile Lys Tyr His Ile Trp Leu Gly His Met Val 290 295 300 Met Ala Leu Phe Thr Val His Gly Leu Cys Tyr Ile Ile Tyr Trp Ala 305 310 315 320 Ser Met His Glu Ile Ser Gin Met lie Met Trp Asp Thr Lys Gly Val 325 330 335 Ser Asn Leu Ala Gly Glu lie Ala Leu Ala Ala Gly Leu Val Met Trp 340 345 350 Ala Thr Thr Tyr Pro Lys lie Arg Arg Arg Phe Phe Glu Val Phe Phe 355 360 365 Tyr Thr His Tyr Leu Tyr lie Val Phe Met Leu Phe Phe Val Leu His 370 375 380 Val Gly lie Ser Phe Ser Phe lie Ala Leu Pro Gly Phe Tyr lie Phe 385 390 395 400 Leu Val Asp Arg Phe Leu Arg Phe Leu Gin Ser Arg Glu Asn Val Arg 405 410 415 Leu Leu Ala Ala Arg lie Leu Pro Ser Asp Thr Met Glu Leu Thr Phe 420 425 430 Ser Lys Asn Ser Lys Leu Val Tyr Ser Pro Thr Ser lie Met Phe Val 435 440 445 Asn lie Pro Ser lie Ser Lys Leu Gin Trp His Pro Phe Thr lie Thr 450 455 460 Ser Ser Ser Lys Leu Glu Pro Glu Lys Leu Ser Ile Val Ile Lys Lys 465 470 475 480 Glu Gly Lys Trp Ser Thr Lys Leu His Gln Arg Leu Ser Ser Ser Asp 485 490 495 Gln Ile Asp Arg Leu Ala Val Ser Val Glu Gly Pro Tyr Gly Pro Ala 500 505 510 Ser Ala Asp Phe Leu Arg His Glu Ala Leu Val Met Val Cys Gly Gly 515 520 525 Ser Gly Ile Thr Pro Phe Ile Ser Val Ile Arg Asp Leu Ile Ala Thr 530 535 540 Ser Gln Lys Glu Thr Cys Lys Ile Pro Lys Ile Thr Leu Ile Cys Ala 545 550 555 560 Phe Lys Lys Ser Ser Glu Ile Ser Met Leu Asp Leu Val Leu Pro Leu 565 570 575 Ser Gly Leu Glu Thr Glu Leu Ser Ser Asp Ile Asn Ile Lys Ile Glu 580 585 590 Ala Phe Ile Thr Arg Asp Asn Asp Ala Gly Asp Glu Ala Lys Ala Gly 595 600 605 Lys Ile Lys Thr Leu Trp Phe Lys Pro Ser Leu Ser Asp Gln Ser Ile 610 615 620 Ser Ser Ile Leu Gly Pro Asn Ser Trp Leu Trp Leu Gly Ala Ile Leu 625 630 635 640 Ala Ser Ser Phe Leu Ile Phe Met Ile Ile Ile Gly Ile Ile Thr Arg 645 650 655 Tyr Tyr Ile Tyr Pro Ile Asp His Asn Thr Asn Lys Ile Tyr Ser Leu 660 665 670 Thr Ser Lys Thr Ile Ile Tyr Ile Leu Val Ile Ser Val Ser Ile Met 675 680 685 Ala Thr Cys Ser Ala Ala Met Leu Trp Asn Lys Lys Lys Tyr Gly Lys 690 695 700 Val Glu Ser Lys Gln Val Gln Asn Val Asp Arg Pro Ser Pro Thr Ser 705 710 715 720 Ser Pro Thr Ser Ser Trp Gly Tyr Asn Ser Leu Arg Glu Ile Glu Ser 725 730 735 Thr Pro Gln Glu Ser Leu Val Gln Arg Thr Asn Leu His Phe Gly Glu 740 745 750 Arg Pro Asn Leu Lys Lys Leu Leu Leu Asp Val Glu Gly Ser Ser Val 755 760 765 Gly Val Leu Val Cys Gly Pro Lys Lys Met Arg Gin Lys Val Ala Glu 770 775 780 Ile Cys Ser Ser Gly Leu Ala Glu Asn Leu His Phe Glu Ser Ile Ser 785 790 795 800 Phe Ser Trp <210> 25 <211> 2374 <212> DNA <213> Medicago truncatula <220> <221> CDS <222> (64)..(2214) <400> 25 gtttcttctt ctctctaaac aattaaattt tcacacacaa ccgagaagaa acaacaacaa 60 aaa atg gct caa gat aaa gtg aaa agg tca cct act caa ata aaa tat 108 Met Ala Gln Asp Lys Val Lys Arg Ser Pro Thr Gln Ile Lys Tyr 1 5 10 15 gga tta att caa tct aca ata agg tta ttg atg gtg tta ctg ttt ctg 156 Gly Leu Ile Gln Ser Thr Ile Arg Leu Leu Met Val Leu Leu Phe Leu 20 25 30 ggt tta att ttc att ttt ata atg atg cct aca tat act ttc aaa cac 204 Gly Leu Ile Phe Ile Phe Ile Met Met Pro Thr Tyr Thr Phe Lys His 35 40 45 aca tgg aat cct caa ttc caa gca aag act aat tct aca tat ttt ggt 252 Thr Trp Asn Pro Gln Phe Gln Ala Lys Thr Asn Ser Thr Tyr Phe Gly 50 55 60 gca caa ggt aca agg att ctt att tac act ttt cca gtg ttg tta ata 300 Ala Gln Gly Thr Arg Ile Leu Ile Tyr Thr Phe Pro Val Leu Leu Ile 65 70 75 gcc act ttg gga tgt gtc ttt ctc cac ata gcc aaa aaa tca aat gag 348 Ala Thr Leu Gly Cys Val Phe Leu His Ile Ala Lys Lys Ser Asn Glu 80 85 90 95 agc aac atg gaa att ggc aat ggg aag aaa cat ggg aca acc ata tgg 396 Ser Asn Met Glu Ile Gly Asn Gly Lys Lys His Gly Thr Thr Ile Trp 100 105 110 aat cgt cca atg ctt gtg aaa gga cct ctt gga att gtt tct att aca 444 Asn Arg Pro Met Leu Val Lys Gly Pro Leu Gly Ile Val Ser Ile Thr 115 120 125 gaa ata gca ttc ttg ctt atg ttc att gca ctt cta att tgg tcc ttt 492 Glu Ile Ala Phe Leu Leu Met Phe Ile Ala Leu Leu Ile Trp Ser Phe 130 135 140 tcc att tat tta cac aac gac ttt gca aca atc ata tca aaa tca gag 540 Ser Ile Tyr Leu His Asn Asp Phe Ala Thr Ile Ile Ser Lys Ser Glu 145 150 155 gca gag aat ggc gaa aaa gtg tgg caa gag aaa ata gaa agt gtg gca 588 Ala Glu Asn Gly Glu Lys Val Trp Gln Glu Lys Ile Glu Ser Val Ala 160 165 170 175 cta agg tta ggt ctt gtt ggt aac ata tgt ttg gca ttg tta ttt ttc 636 Leu Arg Leu Gly Leu Val Gly Asn Ile Cys Leu Ala Leu Leu Phe Phe 180 185 190 cca gtg act cgt gga tca acc gtg cta cca ata ttt ggc ctc act tct 684 Pro Val Thr Arg Gly Ser Thr Val Leu Pro Ile Phe Gly Leu Thr Ser 195 200 205 gaa ggt tgc atc aaa tac cac att tgg ctt ggg cac gtg ctt atg aca 732 Glu Gly Cys Ile Lys Tyr His Ile Trp Leu Gly His Val Leu Met Thr 210 215 220 ttt ttc act act cat gga gtt tgt tac att atc tat tgg gca tct act 780 Leu Phe Thr Thr His Gly Val Cys Tyr Ile Ile Tyr Trp Ala Ser Thr 225 230 235 cat caa att tca cag atg ctg aaa tgg gac aaa gtt gga gta tca aat 828 His Gln Ile Ser Gln Met Leu Lys Trp Asp Lys Val Gly Val Ser Asn 240 245 250 255 gtg gct gga gag ata tct ttg ctt gct ggt tta gtc tta tgg gtt gca 876 Val Ala Gly Glu Ile Ser Leu Leu Ala Gly Leu Val Leu Trp Val Ala 260 265 270 acc att cct tac att agg aga aaa tac ttt gag cta ttt ttc tac act 924 Thr Ile Pro Tyr Ile Arg Arg Lys Tyr Phe Glu Leu Phe Phe Tyr Thr 275 280 285 cat tac ctc tac att atc ttc atc atc ttc ttc atc ttt cat gtt ggc 972 His Tyr Leu Tyr Ile Ile Phe Ile Ile Phe Phe Ile Phe His Val Gly 290 295 300 att tcc ttt gcc tgc att atg ctc cct ggt ttc tac ctc ttc ttg gtt 1020 Ile Ser Phe Ala Cys Ile Met Leu Pro Gly Phe Tyr Leu Phe Leu Val 305 310 315 gat cgt tac cta cgg ttc cta caa tct agg cgc gaa gtt cgt ttg gtt 1068 Asp Arg Tyr Leu Arg Phe Leu Gln Ser Arg Arg Glu Val Arg Leu Val 320 325 330 335 tca tct cgt gtt ttg ccg tgt gaa act gta gaa ctc aac ttc tct aag 1116 Ser Ser Arg Val Leu Pro Cys Glu Thr Val Glu Leu Asn Phe Ser Lys 340 345 350 gga cat gga ttg acg tat aat cca aca agt gtg atg ttc ata aat gta 1164 Gly His Gly Leu Thr Tyr Asn Pro Thr Ser Val Met Phe Ile Asn Val 355 360 365 cca agc ata tca aag ttg caa tgg cat cca ttt acc att act tct aat 1212 Pro Ser Ile Ser Lys Leu Gln Trp His Pro Phe Thr Ile Thr Ser Asn 370 375 380 agt aaa ttg gaa tct gaa aag cta agt gtt gtc att aaa agt gaa gga 1260 Ser Lys Leu Glu Ser Glu Lys Leu Ser Val Val Ile Lys Ser Glu Gly 385 390 395 act tgg aca aaa aag ctc tac cag ttg ctt tca aat cct tcg cct atc 1308 Thr Trp Thr Lys Lys Leu Tyr Gln Leu Leu Ser Asn Pro Ser Pro Ile 400 405 410 415 gat cgc ctt gga ata tct gtt gaa ggt cct tat gga cct gct tca acc 1356 Asp Arg Leu Gly Ile Ser Val Glu Gly Pro Tyr Gly Pro Ala Ser Thr 420 425 430 aat tat cta agg cat gac aca ctt gtg atg gtg agt gga gga agt ggc 1404 Asn Tyr Leu Arg His Asp Thr Leu Val Met Val Ser Gly Gly Ser Gly 435 440 445 atc aca cca ttt atc tct atc att aga gag cta ata tat ctt agc act 1452 Ile Thr Pro Phe Ile Ser Ile Ile Arg Glu Leu Ile Tyr Leu Ser Thr 450 455 460 aca ttc aaa tgc aag aca cca aac att gtc cta att tct tca ttc aag 1500 Thr Phe Lys Cys Lys Thr Pro Asn Ile Val Leu Ile Ser Ser Phe Lys 465 470 475 aat act tca tgt ttg tca atg cta gat ttg atc cta cct att tct ggc 1548 Asn Thr Ser Cys Leu Ser Met Leu Asp Leu Ile Leu Pro Ile Ser Gly 480 485 490 495 aca cca tct gac att tct aat att caa ctc caa att gag gca tac atc 1596 Thr Pro Ser Asp Ile Ser Asn Ile Gln Leu Gln Ile Glu Ala Tyr Ile 500 505 510 aca aga gac aga gag ttt aaa tca gat agc tca att cat cct caa act 1644 Thr Arg Asp Arg Glu Phe Lys Ser Asp Ser Ser Ile His Pro Gln Thr 515 520 525 tta tgg ttt aag cca aat cca aca gat gca cca ata cat tct atg tta 1692 Leu Trp Phe Lys Pro Asn Pro Thr Asp Ala Pro Ile His Ser Met Leu 530 535 540 ggt cca aac act tgg ctt tgg ctt ggt gct ata atc tca tct tct ttc 1740 Gly Pro Asn Thr Trp Leu Trp Leu Gly Ala Ile Ile Ser Ser Ser Phe 545 550 555 att atc ttc ctt atc ata atc ggg atc att act cga tac tac att ttc 1788 Ile Ile Phe Leu Ile Ile Ile Gly Ile Ile Thr Arg Tyr Tyr Ile Phe 560 565 570 575 ccg att gat cat aac acg aat aaa ata ttc tcg tat cct tta agg gtg 1836 Pro Ile Asp His Asn Thr Asn Lys Ile Phe Ser Tyr Pro Leu Arg Val 580 585 590 ttc ctt aac gtg cta gta ata tgt gtg tcg gta gtt gtt gtt gct agt 1884 Phe Leu Asn Val Leu Val Ile Cys Val Ser Val Val Val Val Ala Ser 595 600 605 gta gca gtt att tgg aat aaa aaa caa aat gct aaa gaa gca aaa cag 1932 Val Ala Val Ile Trp Asn Lys Lys Gln Asn Ala Lys Glu Ala Lys Gln 610 615 620 att cag aac ttg gaa ggg tca tca cca aca gtg tca cca agt tca atg 1980 Ile Gln Asn Leu Glu Gly Ser Ser Pro Thr Val Ser Pro Ser Ser Met 625 630 635 att tac aat gtt gat aga gaa tta gaa agc ctt cca tat cag tcc cta 2028 Ile Tyr Asn Val Asp Arg Glu Leu Glu Ser Leu Pro Tyr Gln Ser Leu 640 645 650 655 gtt caa gct acc aat gtg cat tat gga aca aga cct gat ctt aaa aga 2076 Val Gin Ala Thr Asn Val His Tyr Gly Thr Arg Pro Asp Leu Lys Arg 660 665 670 ttt gaa atg aaa ggg tca agt gtg gga gtt ctt gtt tca gga 2124 Leu Leu Phe Glu Met Lys Gly Ser Ser Val Gly Val Leu Val Ser Gly 675 680 685 cct aaa caa atg agg cag gag gtt gca tcc att tgt tca tct ggt tta 2172 Pro Lys Gln Met Arg Gln Glu Val Ala Ser Ile Cys Ser Ser Gly Leu 690 695 700 gtt gaa aat ctg cat ttt gag tcc ata agt ttt acc tgg tga 2214 Val Glu Asn Leu His Phe Glu Ser Ile Ser Phe Thr Trp 705 710 715 ttttattcat tctaattcaa agggtgcaaa ccatccatga tcaatctcta tagtaggact 2274 tcattttaat tgccttgatt aaaaaatctg taaaatttgt ttttcaacaa ataactaggc 2334 tttcttgtat accatatatg acatgaatga agtttaatga 2374 <210> 26 <211> 716 <212> PRT <213> Medicago truncatula <400> 26 Met Ala Gin Asp Lys Val Lys Arg Ser Pro Thr Gin He Lys Tyr Gly 1 5 10 15 Leu He Gin Ser Thr He Arg Leu Leu Met Val Leu Leu Phe Leu Gly 20 25 30 Leu He Phe He Phe He Met Met Pro Thr Tyr Thr Phe Lys His Thr 35 40 45 Trp Asn Pro Gin Phe Gin Ala Lys Thr Asn Ser Thr Tyr Phe Gly Ala 50 55 60 Gin Gly Thr Arg He Leu He Tyr Thr Phe Pro Val Leu Leu He Ala 65 70 75 80 Thr Leu Gly Cys Val Phe Leu His He Ala Lys Lys Ser Asn Gin Ser 85 90 95 Asn Met Glu He Gly Asn Gly Lys Lys His Gly Thr Thr He Trp Asn 100 105 110 Arg Pro Met Leu Val Lys Gly Pro Leu Gly He Val Ser He Thr Gin 115 120 125 He Ala Phe Leu Leu Met Phe He Ala Leu Leu He Trp Ser Phe Ser 130 135 140 He Tyr Leu His Asn Asp Phe Ala Thr He He Ser Lys Ser Gin Ala 145 150 155 160 Glu Asn Gly Glu Lys Val Trp Gin Glu Lys He Glu Ser Val Ala Leu 165 170 175 Arg Leu Gly Leu Val Gly Asn He Cys Leu Ala Leu Leu Phe Phe Pro 180 185 190 Val Thr Arg Gly Ser Thr Val Leu Pro He Phe Gly Leu Thr Ser Glu 195 200 205 Gly Cys He Lys Tyr His He Trp Leu Gly His Val Leu Met Thr Leu 210 215 220 Phe Thr Thr His Gly Val Cys Tyr He He Tyr Trp Ala Ser Thr His 225 230 235 240 Gln He Ser Gin Met Leu Lys Trp Asp Lys Val Gly Val Ser Asn Val 245 250 255 Ala Gly Glu He Ser Leu Leu Ala Gly Leu Val Leu Trp Val Ala Thr 260 265 270 He Pro Tyr He Arg Arg Lys Tyr Phe Glu Leu Phe Phe Tyr Thr His 275 280 285 Tyr Leu Tyr He He Phe He He Phe Phe He Phe His Val Gly He 290 295 300 Ser Phe Ala Cys He Met Leu Pro Gly Phe Tyr Leu Phe Leu Val Asp 305 310 315 320 Arg Tyr Leu Arg Phe Leu Gln Ser Arg Arg Glu Val Arg Leu Val Ser 325 330 335 Ser Arg Val Leu Pro Cys Glu Thr Val Glu Leu Asn Phe Ser Lys Gly 340 345 350 His Gly Leu Thr Tyr Asn Pro Thr Ser Val Met Phe Ile Asn Val Pro 355 360 365 Ser Ile Ser Lys Leu Gln Trp His Pro Phe Thr Ile Thr Ser Asn Ser 370 375 380 Lys Leu Glu Ser Glu Lys Leu Ser Val Val Ile Lys Ser Glu Gly Thr 385 390 395 400 Trp Thr Lys Lys Leu Tyr Gln Leu Leu Ser Asn Pro Ser Pro Ile Asp 405 410 415 Arg Leu Gly Ile Ser Val Glu Gly Pro Tyr Gly Pro Ala Ser Thr Asn 420 425 430 Tyr Leu Arg His Asp Thr Leu Val Met Val Ser Gly Gly Ser Gly Ile 435 440 445 Thr Pro Phe Ile Ser Ile Ile Arg Glu Leu Ile Tyr Leu Ser Thr Thr 450 455 460 Phe Lys Cys Lys Thr Pro Asn Ile Val Leu Ile Ser Ser Phe Lys Asn 465 470 475 480 Thr Ser Cys Leu Ser Met Leu Asp Leu Ile Leu Pro Ile Ser Gly Thr 485 490 495 Pro Ser Asp Ile Ser Asn Ile Gln Leu Gln Ile Glu Ala Tyr Ile Thr 500 505 510 Arg Asp Arg Glu Phe Lys Ser Asp Ser Ser Ile His Pro Gln Thr Leu 515 520 525 Trp Phe Lys Pro Asn Pro Thr Asp Ala Pro Ile His Ser Met Leu Gly 530 535 540 Pro Asn Thr Trp Leu Trp Leu Gly Ala Ile Ile Ser Ser Ser Phe Ile 545 550 555 560 Ile Phe Leu Ile Ile Ile Gly Ile Ile Thr Arg Tyr Tyr Ile Phe Pro 565 570 575 Ile Asp His Asn Thr Asn Lys Ile Phe Ser Tyr Pro Leu Arg Val Phe 580 585 590 Leu Asn Val Leu Val Ile Cys Val Ser Val Val Val Val Ala Ser Val 595 600 605 Ala Val Ile Trp Asn Lys Lys Gln Asn Ala Lys Glu Ala Lys Gln Ile 610 615 620 Gln Asn Leu Glu Gly Ser Ser Pro Thr Val Ser Pro Ser Ser Met Ile 625 630 635 640 Tyr Asn Val Asp Arg Glu Leu Glu Ser Leu Pro Tyr Gln Ser Leu Val 645 650 655 Gln Ala Thr Asn Val His Tyr Gly Thr Arg Pro Asp Leu Lys Arg Leu 660 665 670 Leu Phe Glu Met Lys Gly Ser Ser Val Gly Val Leu Val Ser Gly Pro 675 680 685 Lys Gln Met Arg Gln Glu Val Ala Ser Ile Cys Ser Ser Gly Leu Val 690 695 700 Glu Asn Leu His Phe Glu Ser Ile Ser Phe Thr Trp 705 710 715 <210> 27 <211> 2406 <212> DNA <213> Sunflower[[ID=3​​​​​​​​​​​​​​​​​​​​ggc tat ctt ttc cta tgg gtg atc aca cca acc tat gtt ttt aag aaa 152 Gly Tyr Leu Phe Leu Trp Val Ile Thr Pro Thr Tyr Val Phe Lys Lys 20 25 30 aag tgg atc ctt agg gtt cga gcc cac acc act tct act tac ttt gga 200 Lys Trp Ile Leu Arg Val Arg Ala His Thr Thr Ser Thr Tyr Phe Gly 35 40 45 act caa ggt cca acg atg ttg ctc tac aca ttg ccc gtt ttg atc atg 248 Thr Gln Gly Pro Thr Met Leu Leu Tyr Thr Leu Pro Val Leu Ile Met 50 55 60 65 gcc gtc ttg gga agt gtg tat ctc cat tta aga aaa aag gcg aac caa 296 Ala Val Leu Gly Ser Val Tyr Leu His Leu Arg Lys Lys Ala Asn Gln 70 75 80 aac cgc gac gaa gga gat aaa gaa gct act tcc gat aac ttg agt ata 344 Asn Arg Asp Glu Gly Asp Lys Glu Ala Thr Ser Asp Asn Leu Ser Ile 85 90 95 tgg aaa agg cca atg atc atc aaa ggt ctc gga atc gtc tcg ata atc 392 Trp Lys Arg Pro Met Ile Ile Lys Gly Leu Gly Ile Val Ser Ile Ile 100 105 110 gag ctc gct ttc ttc gtt atg ttt atc gct ctc att gtc tgg tct ttc 440 Glu Leu Ala Phe Phe Val Met Phe Ile Ala Leu Ile Val Trp Ser Phe 115 120 125 aca aca tac tta cat gtc agt ttt gcc ggt att aac cat aaa aca gca 488 Thr Thr Tyr Leu His Val Ser Phe Ala Gly Ile Asn His Lys Thr Ala 130 135 140 145 gca aaa aat gga gaa gaa gtt tgg caa tca aag ttg agt tca gtg gct 536 Ala Lys Asn Gly Glu Glu Val Trp Gln Ser Lys Leu Ser Ser Val Ala 150 155 160 cta cgg ttc ggg tta att ggt aac ttg tgt cta gta ttt ttg ttc ttc 584 Leu Arg Phe Gly Leu Ile Gly Asn Leu Cys Leu Val Phe Leu Phe Phe 165 170 175 cca gtg act cga gga tcg tct ata ttg tcg ctg ttc ggg cta act tct 632 Pro Val Thr Arg Gly Ser Ser Ile Leu Ser Leu Phe Gly Leu Thr Ser 180 185 190 gag gcc agc atc aag tat cac ata tgg ctg ggc cat att gtt atg acc 680 Glu Ala Ser Ile Lys Tyr His Ile Trp Leu Gly His Ile Val Met Thr 195 200 205 ctc ttc act tct cat ggt gtt tgt tac att att tat tgg ata gta acc 728 Leu Phe Thr Ser His Gly Val Cys Tyr Ile Ile Tyr Trp Ile Val Thr 210 215 220 225 aag cag aca tca gag atg gtg aaa tgg gca aaa acc gac ata tca aat 776 Lys Gln Thr Ser Glu Met Val Lys Trp Ala Lys Thr Asp Ile Ser Asn 230 235 240 gta gcc gga gag ttg gct ttg gta gca gga ttg att atg tgg gca acg 824 Val Ala Gly Glu Leu Ala Leu Val Ala Gly Leu Ile Met Trp Ala Thr 245 250 255 acg ttt cct agg att aga cga aag atg ttt gag gtt ttc ttc tac acg 872 Thr Phe Pro Arg Ile Arg Arg Lys Met Phe Glu Val Phe Phe Tyr Thr 260 265 270 cat cac ttg tac atc cta ttt gtc gtg ttc ttc gtg ttc cat gtt ggc 920 His His Leu Tyr Ile Leu Phe Val Val Phe Phe Val Phe His Val Gly 275 280 285 atg atg ctt cct gga ttc tac ctt ttc atg att 968 Ile Ala Tyr Ala Ser Met Met Leu Pro Gly Phe Tyr Leu Phe Met Ile 290 295 300 305 gat cgg tac ttg aga ttt tta caa tca aaa ggg aag gtt cgt ttg gtt 1016 Asp Arg Tyr Leu Arg Phe Leu Gln Ser Lys Gly Lys Val Arg Leu Val 310 315 320 tct tcg cgt gtt ctt cca tgt gaa act ctc gag ctc aac ttc tcc aag 1064 Ser Ser Arg Val Leu Pro Cys Glu Thr Leu Glu Leu Asn Phe Ser Lys 325 330 335 agc cga ggt ttg aat tat aca cca aca agc atc ata ttc atc aat atc 1112 Ser Arg Gly Leu Asn Tyr Thr Pro Thr Ser Ile Ile Phe Ile Asn Ile 340 345 350 cca agc att tca aag gct caa tgg cat cca ttc acc att act tca agt 1160 Pro Ser Ile Ser Lys Ala Gln Trp His Pro Phe Thr Ile Thr Ser Ser 355 360 365 agc aat ttg gag cct gaa aag cta agt gtt atg atc aaa agt gaa ggg 1208 Ser Asn Leu Glu Pro Glu Lys Leu Ser Val Met Ile Lys Ser Glu Gly 370 375 380 385 agt tgg tct agg aaa ctc tac caa atg tta tca tcc ccg aat tct ata 1256 Ser Trp Ser Arg Lys Leu Tyr Gln Met Leu Ser Ser Pro Asn Ser Ile 390 395 400 gat cgg ctt gat gtg tgc gtt gaa gga cct tat gga ccc gtt gca acc 1304 Asp Arg Leu Asp Val Cys Val Glu Gly Pro Tyr Gly Pro Val Ala Thr 405 410 415 aat ttt cta agg cac gat atg cta gtg atg gtg agc gga gga agt ggg 1352 Asn Phe Leu Arg His Asp Met Leu Val Met Val Ser Gly Gly Ser Gly 420 425 430 ata aca ccg ttt atc tcg ata ttt agg gag ctt gtt tac aca acc gaa 1400 Ile Thr Pro Phe Ile Ser Ile Phe Arg Glu Leu Val Tyr Thr Thr Glu 435 440 445 acc cta aaa tgt aaa gct cca aaa att ctc ttg ata agt gcg ttt aaa 1448 Thr Leu Lys Cys Lys Ala Pro Lys Ile Leu Leu Ile Ser Ala Phe Lys 450 455 460 465 aac tca tct gat ctt acc atg cta gaa ttg tta tta cca tca tcc ggt 1496 Asn Ser Ser Asp Leu Thr Met Leu Glu Leu Leu Leu Pro Ser Ser Gly 470 475 480 tca cct ata gag ttt tca aaa cta gag tta caa atc gag gcc tat gta 1544 Ser Pro Ile Glu Phe Ser Lys Leu Glu Leu Gln Ile Glu Ala Tyr Val 485 490 495 aca aga gag aaa caa ccc gtg acc gat gat aag aaa cta gtg aat acc 1592 Thr Arg Glu Lys Gln Pro Val Thr Asp Asp Lys Lys Leu Val Asn Thr 500 505 510 ctt tgg ctt aaa cca aac ccc tcc gat gcg cac ata act cct gtt tta 1640 Leu Trp Leu Lys Pro Asn Pro Ser Asp Ala His Ile Thr Pro Val Leu 515 520 525 gga caa aat ggt tgg tta tgg ctt ggc gca att att gta tct tct ttt 1688 Gly Gln Asn Gly Trp Leu Trp Leu Gly Ala Ile Ile Val Ser Ser Phe 530 535 540 545 gtg gtt ttc ctt gtg tct atg gga ctt gta acg cgc ttt tac atc tat 1736 Val Val Phe Leu Val Ser Met Gly Leu Val Thr Arg Phe Tyr Ile Tyr 550 555 560 cct att gat aag aac aca aac aaa gtg tac tca cta gga tcg agg gcc 1784 Pro Ile Asp Lys Asn Thr Asn Lys Val Tyr Ser Leu Gly Ser Arg Ala 565 570 575 gcg tta aat atg ttg ctc att tgt gtg tcg att atg gta aca tgt tat 1832 Ala Leu Asn Met Leu Leu Ile Cys Val Ser Ile Met Val Thr Cys Tyr 580 585 590 atg ggt ttt tgg tgg aat aag aag aga aat gct atg gat tca aag caa 1880 Met Gly Phe Trp Trp Asn Lys Lys Arg Asn Ala Met Asp Ser Lys Gln 595 600 605 att caa aac atg gag ggg gcg aca ccc gtt aac tca ccc aac tca tgg 1928 Ile Gln Asn Met Glu Gly Ala Thr Pro Val Asn Ser Pro Asn Ser Trp 610 615 620 625 ttt tat aac gcg gat aga gag ctt gaa agc atg cct caa caa tca ctt 1976 Phe Tyr Asn Ala Asp Arg Glu Leu Glu Ser Met Pro Gin Gin Ser Leu 630 635 640 tat cag tct aca aat gtg cat ttt ggc aaa agg ccg gat ctt aag aga 2024 Tyr Gin Ser Thr Asn Val His Phe Gly Lys Arg Pro Asp Leu Lys Arg 645 650 655 att atg ttt gag caa aaa gaa tca agt gtt gga gtt cta gtt tgt ggg 2072 Ile Met Phe Glu Gin Lys Glu Ser Ser Val Gly Val Leu Val Cys Gly 660 665 670 cca aag aaa atg agg cat gag gtt gca aac ata tgt tca tct gga ttg 2120 Pro Lys Lys Met Arg His Glu Val Ala Asn Ile Cys Ser Ser Gly Leu 675 680 685 gct tca aac ttg cat ttt gag tcc ata agc ttt agc tgg tga 2162 Ala Ser Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 690 695 700 tcgactggtc ggcttttgca aattgcaaaa catggtgtgc gacgagttgg tttttctttt 2222 catattcgtc acttacaagt tttgatgatt caaagttcaa ggtttcagtt ttgttccgat 2282 agagcgtcca aaatatagaa atgtgtgtaa atcggataca agggtgcctt atctttcctt 2342 ttgcttgtaa acttgtaatg cataaataaa ctgtttatat atcaaacttt ttgtatttaa 2402 atta 2406 <210> 28 <211> 702 <212> PRT <213> Helianthus annuus <400> 28 Met Met Met Leu Met Lys Met Ile Lys Gly Leu Ala Met Met Val Phe 1 5 10 15 Ile Gly Tyr Leu Phe Leu Trp Val Ile Thr Pro Thr Tyr Val Phe Lys 20 25 30 Lys Lys Trp Ile Leu Arg Val Arg Ala His Thr Thr Ser Thr Tyr Phe 35 40 45 Gly Thr Gln Gly Pro Thr Met Leu Leu Tyr Thr Leu Pro Val Leu Ile 50 55 60 Met Ala Val Leu Gly Ser Val Tyr Leu His Leu Arg Lys Lys Ala Asn 65 70 75 80 Gln Asn Arg Asp Glu Gly Asp Lys Glu Ala Thr Ser Asp Asn Leu Ser 85 90 95 Ile Trp Lys Arg Pro Met Ile Ile Lys Gly Leu Gly Ile Val Ser Ile 100 105 110 Ile Glu Leu Ala Phe Phe Val Met Phe Ile Ala Leu Ile Val Trp Ser 115 120 125 Phe Thr Thr Tyr Leu His Val Ser Phe Ala Gly Ile Asn His Lys Thr 130 135 140 Ala Ala Lys Asn Gly Glu Glu Val Trp Gin Ser Lys Leu Ser Ser Val 145 150 155 160 Ala Leu Arg Phe Gly Leu Ile Gly Asn Leu Cys Leu Val Phe Leu Phe 165 170 175 Phe Pro Val Thr Arg Gly Ser Ser Ile Leu Ser Leu Phe Gly Leu Thr 180 185 190 Ser Glu Ala Ser Ile Lys Tyr His Ile Trp Leu Gly His Ile Val Met 195 200 205 Thr Leu Phe Thr Ser His Gly Val Cys Tyr Ile Ile Tyr Trp Ile Val 210 215 220 Thr Lys Gin Thr Ser Glu Met Val Lys Trp Ala Lys Thr Asp Ile Ser 225 230 235 240 Asn Val Ala Gly Glu Leu Ala Leu Val Ala Gly Leu Ile Met Trp Ala 245 250 255 Thr Thr Phe Pro Arg Ile Arg Arg Lys Met Phe Glu Val Phe Phe Tyr 260 265 270 Thr His His Leu Tyr Ile Leu Phe Val Val Phe Phe Val Phe His Val 275 280 285 Gly Ile Ala Tyr Ala Ser Met Met Leu Pro Gly Phe Tyr Leu Phe Met 290 295 300 Ile Asp Arg Tyr Leu Arg Phe Leu Gln Ser Lys Gly Lys Val Arg Leu 305 310 315 320 Val Ser Ser Arg Val Leu Pro Cys Glu Thr Leu Glu Leu Asn Phe Ser 325 330 335 Lys Ser Arg Gly Leu Asn Tyr Thr Pro Thr Ser Ile Ile Phe Ile Asn 340 345 350 Ile Pro Ser Ile Ser Lys Ala Gln Trp His Pro Phe Thr Ile Thr Ser 355 360 365 Ser Ser Asn Leu Glu Pro Glu Lys Leu Ser Val Met Ile Lys Ser Glu 370 375 380 Gly Ser Trp Ser Arg Lys Leu Tyr Gln Met Leu Ser Ser Pro Asn Ser 385 390 395 400 Ile Asp Arg Leu Asp Val Cys Val Glu Gly Pro Tyr Gly Pro Val Ala 405 410 415 Thr Asn Phe Leu Arg His Asp Met Leu Val Met Val Ser Gly Gly Ser 420 425 430 Gly Ile Thr Pro Phe Ile Ser Ile Phe Arg Glu Leu Val Tyr Thr Thr 435 440 445 Glu Thr Leu Lys Cys Lys Ala Pro Lys Ile Leu Leu Ile Ser Ala Phe 450 455 460 Lys Asn Ser Ser Asp Leu Thr Met Leu Glu Leu Leu Leu Pro Ser Ser 465 470 475 480 Gly Ser Pro Ile Glu Phe Ser Lys Leu Glu Leu Gln Ile Glu Ala Tyr 485 490 495 Val Thr Arg Glu Lys Gln Pro Val Thr Asp Asp Lys Lys Leu Val Asn 500 505 510 Thr Leu Trp Leu Lys Pro Asn Pro Ser Asp Ala His Ile Thr Pro Val 515 520 525 Leu Gly Gln Asn Gly Trp Leu Trp Leu Gly Ala Ile Ile Val Ser Ser 530 535 540 Phe Val Val Phe Leu Val Ser Met Gly Leu Val Thr Arg Phe Tyr Ile 545 550 555 560 Tyr Pro Ile Asp Lys Asn Thr Asn Lys Val Tyr Ser Leu Gly Ser Arg 565 570 575 Ala Ala Leu Asn Met Leu Leu Ile Cys Val Ser Ile Met Val Thr Cys 580 585 590 Tyr Met Gly Phe Trp Trp Asn Lys Lys Arg Asn Ala Met Asp Ser Lys 595 600 605 Gln Ile Gln Asn Met Glu Gly Ala Thr Pro Val Asn Ser Pro Asn Ser 610 615 620 Trp Phe Tyr Asn Ala Asp Arg Glu Leu Glu Ser Met Pro Gln Gln Ser 625 630 635 640 Leu Tyr Gln Ser Thr Asn Val His Phe Gly Lys Arg Pro Asp Leu Lys 645 650 655 Arg Ile Met Phe Glu Gln Lys Glu Ser Ser Val Gly Val Leu Val Cys 660 665 670 Gly Pro Lys Lys Met Arg His Glu Val Ala Asn Ile Cys Ser Ser Gly 675 680 685 Leu Ala Ser Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 690 695 700 <210> 29 <211> 2254 <212> DNA <213> Cassava <220> <221> CDS <222> (1)..(2244) <400> 29 atg gat tca gaa tca gct tct aac aca gaa agt aac cat atg gtt cgg 48 Met Asp Ser Glu Ser Ala Ser Asn Thr Glu Ser Asn His Met Val Arg 1 5 10 15 aca ggg att aag aca aca ttg tgg gta gta ctg gga gga tat gct gtg 96 Thr Gly Ile Lys Thr Thr Leu Trp Val Val Leu Gly Gly Tyr Ala Val 20 25 30 ttt tgg gtt atg atg ccc act aat acc tac caa caa aca tgg cgt tct 144 Phe Trp Val Met Met Pro Thr Asn Thr Tyr Gln Gln Thr Trp Arg Ser 35 40 45 tat ttg aga gtt cat atc gat tcc acc tac ttt ggg act caa gct aat 192 Tyr Leu Arg Val His Ile Asp Ser Thr Tyr Phe Gly Thr Gln Ala Asn 50 55 60 atg ttt cct tgt att tct tcc ctt gct ttc gtt tca atg act ggt ttt 240 Met Phe Pro Cys Ile Ser Ser Leu Ala Phe Val Ser Met Thr Gly Phe 65 70 75 80 att tta cgg cta tgg ggg ctt tct gct tat gca tgt aaa ctt gtt tca 288 Ile Leu Arg Leu Trp Gly Leu Ser Ala Tyr Ala Cys Lys Leu Val Ser 85 90 95 ggt cca aac ctt ttg gta cat ttc gcc acc att ctt ttc att gct ttg 336 Gly Pro Asn Leu Leu Val His Phe Ala Thr Ile Leu Phe Ile Ala Leu 100 105 110 ctg ggt tgt gtt tat ctc cat gta gta aag aga tca aat cac agt aac 384 Leu Gly Cys Val Tyr Leu His Val Val Lys Arg Ser Asn His Ser Asn 115 120 125 ttc gaa agg tac aat ggc aga aaa caa ctc ttg gcc aca tgg aag aag 432 Phe Glu Arg Tyr Asn Gly Arg Lys Gln Leu Leu Ala Thr Trp Lys Lys 130 135 140 ccg atg cta gtg aaa ggc cct ctg gga atc gtt tct gga ata gag ctg 480 Pro Met Leu Val Lys Gly Pro Leu Gly Ile Val Ser Gly Ile Glu Leu 145 150 155 160 gcc ttc ttg atc atg ttt gtt gca ctg ttg gtt tgg tct tta tca acc 528 Ala Phe Leu Ile Met Phe Val Ala Leu Leu Val Trp Ser Leu Ser Thr 165 170 175 tat ttg cat gac agc ttt tct acc atc acc cca cag tcg gct gca ggg 576 Tyr Leu His Asp Ser Phe Ser Thr Ile Thr Pro Gln Ser Ala Ala Gly 180 185 190 agt ggg gag act gta tgg gaa gct aaa atg gag aac gca gcc ctt agg 624 Ser Gly Glu Thr Val Trp Glu Ala Lys Met Glu Asn Ala Ala Leu Arg 195 200 205 cta ggg ttg gtg ggg aac ata tgt ctg acg ttg ctc ttc ttc ccg gtg 672 Leu Gly Leu Val Gly Asn Ile Cys Leu Thr Leu Leu Phe Phe Pro Val 210 215 220 act cga ggg tca tcg att ctg cca ttg ttt ggg ctc acc tca gag ggt 720 Thr Arg Gly Ser Ser Ile Leu Pro Leu Phe Gly Leu Thr Ser Glu Gly 225 230 235 240 agc att aag tac cac ata tgg ctt ggt cac act gtg atg gct ttc ttc 768 Ser Ile Lys Tyr His Ile Trp Leu Gly His Thr Val Met Ala Phe Phe 245 250 255 acc gca cat gga gtt tgt tac atc atc tat tgg gct gct acc gat cag 816 Thr Ala His Gly Val Cys Tyr Ile Ile Tyr Trp Ala Ala Thr Asp Gln 260 265 270 att tcc gag atg ctg aaa tgg caa aag gtt ggg ata tca aat gtg gct 864 Ile Ser Glu Met Leu Lys Trp Ala Lys Val Gly Ile Ser Asn Val Ala 275 280 285 ggg gag att gct ttg ctt gct ggg ctg ggc ctg tgg gct aca acc ttc 912 Gly Glu Ile Ala Leu Leu Ala Gly Leu Gly Leu Trp Ala Thr Thr Phe 290 295 300 act cgc ata aga aga aaa atg ttt gag ctc ttc ttt tac act cat cat 960 Thr Arg Ile Arg Arg Lys Met Phe Glu Leu Phe Phe Tyr Thr His His 305 310 315 320 ctc tac atc ctc ttc atg att ttc tat gta tta cat gtt ccc atc tct 1008 Leu Tyr Ile Leu Phe Met Ile Phe Tyr Val Leu His Val Pro Ile Ser 325 330 335 ttt gcc tgt att aca atc cct gga ttt tac ctc ttc ttg gtc gat cgt 1056 Phe Ala Cys Ile Thr Ile Pro Gly Phe Tyr Leu Phe Leu Val Asp Arg 340 345 350 ttt ctg agg ttc tta caa tca cga caa aga gtt cgt gct gtc tca tcc 1104 Phe Leu Arg Phe Leu Gin Ser Arg Gin Arg Val Arg Ala Val Ser Ser 355 360 365 cgc att ctg cct tgc gaa act tta gaa atc aac ttc tcc aaa aac cct 1152 Arg Ile Leu Pro Cys Glu Thr Leu Glu Ile Asn Phe Ser Lys Asn Pro 370 375 380 ggt ttg agt ttc aat cca acg agc atc ctg ttt ata aat gtg cct agc 1200 Gly Leu Ser Phe Asn Pro Thr Ser Ile Leu Phe Ile Asn Val Pro Ser 385 390 395 400 att tct aag ctg caa tgg cat cct ttc aca atc act tct aat agt aac 1248 Ile Ser Lys Leu Gin Trp His Pro Phe Thr Ile Thr Ser Asn Ser Asn 405 410 415 tta gaa cct gag atg ctg agt gtt atg att aaa agt gaa gga agt tgg 1296 Leu Glu Pro Glu Met Leu Ser Val Met Ile Lys Ser Glu Gly Ser Trp 420 425 430 tcc agg aag ctg cat cag atg ctt tca tct cct tcc tca att gat cgt 1344 Ser Arg Lys Leu His Gin Met Leu Ser Ser Pro Ser Ser Ile Asp Arg 435 440 445 ctt gaa gtt tca gtt gaa gga cct tat gga cct gct tca act cac ttt 1392 Leu Glu Val Ser Val Glu Gly Pro Tyr Gly Pro Ala Ser Thr His Phe 450 455 460 cta agg cat gac aca ctt gtg atg gtg agt gga ggc agt ggc att act 1440 Leu Arg His Asp Thr Leu Val Met Val Ser Gly Gly Ser Gly Ile Thr 465 470 475 480 cct ttt ata tct ata att cga gaa ctc atc ttt gct tcc aca aca tac 1488 Pro Phe Ile Ser Ile Ile Arg Glu Leu Ile Phe Ala Ser Thr Thr Tyr 485 490 495 aaa tca aag att cca caa gta ctc cta att tgc tca ttc aag aac tct 1536 Lys Ser Lys Ile Pro Gln Val Leu Leu Ile Cys Ser Phe Lys Asn Ser 500 505 510 tca gac ctg acc atg cta gat ctc cta ctt cca ata tct ggc acc cca 1584 Ser Asp Leu Thr Met Leu Asp Leu Leu Leu Pro Ile Ser Gly Thr Pro 515 520 525 tca gaa att tcc agt ctg cag cta aaa atc gag gct tat gtt acg aga 1632 Ser Glu Ile Ser Ser Leu Gin Leu Lys Ile Glu Ala Tyr Val Thr Arg 530 535 540 gag aaa gaa ccc tca acc agc aac ggg aag ctc ctt cga act cta tgg 1680 Glu Lys Glu Pro Ser Thr Ser Asn Gly Lys Leu Leu Arg Thr Leu Trp 545 550 555 560 ttc aag ccc cac cca aca gat aaa cct gtt tct gcc att tta ggc ccc 1728 Phe Lys Pro His Pro Thr Asp Lys Pro Val Ser Ala Ile Leu Gly Pro 565 570 575 aaa agc tgg cta tgg ctt ggc gcg ata ata tca tct tcg ttt atc att 1776 Lys Ser Trp Leu Trp Leu Gly Ala Ile Ile Ser Ser Ser Phe Ile Ile 580 585 590 ttc ctc atc ata att ggc ctt att acc cgc tat tac att tat cct atc 1824 Phe Leu Ile Ile Ile Gly Leu Ile Thr Arg Tyr Tyr Ile Tyr Pro Ile 595 600 605 gat cat aac acc tcc agt att ttc tca tac tct ttc cga tct ttt ctt 1872 Asp His Asn Thr Ser Ser Ile Phe Ser Tyr Ser Phe Arg Ser Phe Leu 610 615 620 aat atg tta gta ata tgc ata tgc ata gcc ata aca gca agt gca gcc 1920 Asn Met Leu Val Ile Cys Ile Cys Ile Ala Ile Thr Ala Ser Ala Ala 625 630 635 640 ttt tta tgg aac aag aaa ata aat gga aag caa ccg aat cag att cag 1968 Phe Leu Trp Asn Lys Lys Ile Asn Gly Lys Gln Pro Asn Gln Ile Gln 645 650 655 aac att gaa ggg tcc aca cca aat aca tca cca ggc tca tgg gta tat 2016 Asn Ile Glu Gly Ser Thr Pro Asn Thr Ser Pro Gly Ser Trp Val Tyr 660 665 670 gat ggt ggg aga gaa ttg gaa agt ctt cca cat cag tct ctt gtc cag 2064 Asp Gly Gly Arg Glu Leu Glu Ser Leu Pro His Gln Ser Leu Val Gln 675 680 685 gct act aat gtg cac tat ggc gaa aga cct gat ctg aag aga aca cta 2112 Ala Thr Asn Val His Tyr Gly Glu Arg Pro Asp Leu Lys Arg Thr Leu 690 695 700 ctt gat tgc aaa gga tca agt gtg ggg gtt ctt gtt tgt ggt ccg aag 2160 Leu Asp Cys Lys Gly Ser Ser Val Gly Val Leu Val Cys Gly Pro Lys 705 710 715 720 cag atg agg cat gag att gca atg ata tgt tca tct gga ttg gca gat 2208 Gln Met Arg His Glu Ile Ala Met Ile Cys Ser Ser Gly Leu Ala Asp 725 730 735 aac ttg cac ttt gag tcc att agc ttt agc tgg tga tctagtcatc 2254 Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 740 745 <210> 30 <211> 747 <212> PRT <213> Manihot <400> 30 Met Asp Ser Glu Ser Ala Ser Asn Thr Glu Ser Asn His Met Val Arg 1 5 10 15 Thr Gly Ile Lys Thr Thr Leu Trp Val Val Leu Gly Gly Tyr Ala Val 20 25 30 Phe Trp Val Met Met Pro Thr Asn Thr Tyr Gln Gln Thr Trp Arg Ser 35 40 45 Tyr Leu Arg Val His Ile Asp Ser Thr Tyr Phe Gly Thr Gln Ala Asn 50 55 60 Met Phe Pro Cys Ile Ser Ser Leu Ala Phe Val Ser Met Thr Gly Phe 65 70 75 80 Ile Leu Arg Leu Trp Gly Leu Ser Ala Tyr Ala Cys Lys Leu Val Ser 85 90 95 Gly Pro Asn Leu Leu Val His Phe Ala Thr Ile Leu Phe Ile Ala Leu 100 105 110 Leu Gly Cys Val Tyr Leu His Val Val Lys Arg Ser Asn His Ser Asn 115 120 125 Phe Glu Arg Tyr Asn Gly Arg Lys Gln Leu Leu Ala Thr Trp Lys Lys 130 135 140 Pro Met Leu Val Lys Gly Pro Leu Gly Ile Val Ser Gly Ile Glu Leu 145 150 155 160 Ala Phe Leu Ile Met Phe Val Ala Leu Leu Val Trp Ser Leu Ser Thr 165 170 175 Tyr Leu His Asp Ser Phe Ser Thr Ile Thr Pro Gln Ser Ala Ala Gly 180 185 190 Ser Gly Glu Thr Val Trp Glu Ala Lys Met Glu Asn Ala Ala Leu Arg 195 200 205 Leu Gly Leu Val Gly Asn Ile Cys Leu Thr Leu Leu Phe Phe Pro Val 210 215 220 Thr Arg Gly Ser Ser Ile Leu Pro Leu Phe Gly Leu Thr Ser Glu Gly 225 230 235 240 Ser Ile Lys Tyr His Ile Trp Leu Gly His Thr Val Met Ala Phe Phe 245 250 255 Thr Ala His Gly Val Cys Tyr Ile Ile Tyr Trp Ala Ala Thr Asp Gln 260 265 270 Ile Ser Glu Met Leu Lys Trp Ala Lys Val Gly Ile Ser Asn Val Ala 275 280 285 Gly Glu Ile Ala Leu Leu Ala Gly Leu Gly Leu Trp Ala Thr Thr Phe 290 295 300 Thr Arg Ile Arg Arg Lys Met Phe Glu Leu Phe Phe Tyr Thr His His 305 310 315 320 Leu Tyr Ile Leu Phe Met Ile Phe Tyr Val Leu His Val Pro Ile Ser 325 330 335 Phe Ala Cys Ile Thr Ile Pro Gly Phe Tyr Leu Phe Leu Val Asp Arg 340 345 350 Phe Leu Arg Phe Leu Gln Ser Arg Gln Arg Val Arg Ala Val Ser Ser 355 360 365 Arg Ile Leu Pro Cys Glu Thr Leu Glu Ile Asn Phe Ser Lys Asn Pro 370 375 380 Gly Leu Ser Phe Asn Pro Thr Ser Ile Leu Phe Ile Asn Val Pro Ser 385 390 395 400 Ile Ser Lys Leu Gin Trp His Pro Phe Thr Ile Thr Ser Asn Ser Asn 405 410 415 Leu Glu Pro Glu Met Leu Ser Val Met Ile Lys Ser Glu Gly Ser Trp 420 425 430 Ser Arg Lys Leu His Gin Met Leu Ser Ser Pro Ser Ser Ile Asp Arg 435 440 445 Leu Glu Val Ser Val Glu Gly Pro Tyr Gly Pro Ala Ser Thr His Phe 450 455 460 Leu Arg His Asp Thr Leu Val Met Val Ser Gly Gly Ser Gly Ile Thr 465 470 475 480 Pro Phe Ile Ser Ile Ile Arg Glu Leu Ile Phe Ala Ser Thr Thr Tyr 485 490 495 Lys Ser Lys Ile Pro Gin Val Leu Leu Ile Cys Ser Phe Lys Asn Ser 500 505 510 Ser Asp Leu Thr Met Leu Asp Leu Leu Leu Pro Ile Ser Gly Thr Pro 515 520 525 Ser Glu Ile Ser Ser Leu Gin Leu Lys Ile Glu Ala Tyr Val Thr Arg 530 535 540 Glu Lys Glu Pro Ser Thr Ser Asn Gly Lys Leu Leu Arg Thr Leu Trp 545 550 555 560 Phe Lys Pro His Pro Thr Asp Lys Pro Val Ser Ala Ile Leu Gly Pro 565 570 575 Lys Ser Trp Leu Trp Leu Gly Ala Ile Ile Ser Ser Ser Phe Ile Ile 580 585 590 Phe Leu Ile Ile Ile Gly Leu Ile Thr Arg Tyr Tyr Ile Tyr Pro Ile 595 600 605 Asp His Asn Thr Ser Ser Ile Phe Ser Tyr Ser Phe Arg Ser Phe Leu 610 615 620 Asn Met Leu Val Ile Cys Ile Cys Ile Ala Ile Thr Ala Ser Ala Ala 625 630 635 640 Phe Leu Trp Asn Lys Lys Ile Asn Gly Lys Gln Pro Asn Gln Ile Gln 645 650 655 Asn Ile Glu Gly Ser Thr Pro Asn Thr Ser Pro Gly Ser Trp Val Tyr 660 665 670 Asp Gly Gly Arg Glu Leu Glu Ser Leu Pro His Gln Ser Leu Val Gln 675 680 685 Ala Thr Asn Val His Tyr Gly Glu Arg Pro Asp Leu Lys Arg Thr Leu 690 695 700 Leu Asp Cys Lys Gly Ser Ser Val Gly Val Leu Val Cys Gly Pro Lys 705 710 715 720 Gln Met Arg His Glu Ile Ala Met Ile Cys Ser Ser Gly Leu Ala Asp 725 730 735 Asn Leu His Phe Glu Ser Ile Ser Phe Ser Trp 740 745

Claims

1. A dried leaf of a stevia plant, characterized in that, The genotype of the plant body at position 37 of sequence number 1 is C / C.

2. The dried leaf according to claim 1, characterized in that, The plant body, relative to the control stevia plant body with genotype C / T at position 37 of sequence number 1, contains more of at least one of the nutrients selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiin D and ribobadiin M, and / or has a lower expression level of FRO2 than the control stevia plant body.

3. The dried leaf according to claim 1 or 2, characterized in that, The plant body is a non-GMO plant body.

4. A method for preparing a stevia plant, comprising a stevia plant with a genotype of C / T at position 37 of sequence number 1, containing more of at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobandi glycoside D and ribobandi glycoside M, and / or having a lower expression level of FRO2 than the control stevia plant, characterized in that, The procedure includes crossing a stevia plant with a genotype of C / C at position 37 of sequence number 1 with a second stevia plant.

5. The method according to claim 4, characterized in that, The second plant body is a stevia plant body with the genotype C / C at position 37 of sequence number 1.

6. An extract of a stevia plant with a genotype of C / C at position 37 of sequence number 1, characterized in that, It contains at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M.

7. An extract of tissue from a stevia plant with a genotype of C / C at position 37 of sequence number 1, characterized in that, It contains at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M.

8. An extract of a tissue culture of a stevia plant with a genotype of C / C at position 37 of sequence number 1, characterized in that, It contains at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M.

9. An extract of cells from a stevia plant with a genotype of C / C at position 37 of sequence number 1, characterized in that, It contains at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M.

10. A method for manufacturing an extract containing at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M, characterized in that, The process includes obtaining an extract from a stevia plant with a genotype of C / C at position 37 of sequence number 1.

11. A method for manufacturing an extract containing at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M, characterized in that, The process includes obtaining extracts from tissues of stevia plants with genotype C / C at position 37 of sequence number 1.

12. A method for manufacturing an extract containing at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M, characterized in that, The process includes obtaining an extract from a tissue culture of a stevia plant with a genotype of C / C at position 37 of sequence number 1.

13. A method for manufacturing an extract containing at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M, characterized in that, The process includes obtaining an extract from cells of a stevia plant with a genotype of C / C at position 37 of sequence number 1.

14. A method for manufacturing a food, sweetener composition, flavoring, or pharmaceutical product, characterized in that, The process includes providing the extract according to any one of claims 6 to 9, and, The process of adding the extract to the raw materials of food, sweetener compositions, flavorings or pharmaceuticals.

15. A herbal tea product, characterized in that, The dried body of a stevia plant with a genotype of C / C at position 37 corresponding to sequence number 1.

16. A method for screening stevia plants with a genotype of C / C at position 37 of sequence number 1, characterized in that, It includes a procedure to detect whether the genotype at the position corresponding to position 37 of sequence number 1 in the tested stevia plant is C / C.

17. The method according to claim 16, characterized in that, The process of detecting genetic traits is carried out using sequencing, dCAPS method, or TaqMan PCR method.

18. The method according to claim 16 or 17, characterized in that, The method further includes a step of determining the content of at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobadiol D, and ribobadiol M in the tested stevia plant tissue.

19. A screening kit for stevia plants with a genotype of C / C at position 37 of sequence number 1, characterized in that, It contains a reagent for detecting whether the genotype at the position corresponding to position 37 of sequence number 1 is C / C.

20. The reagent kit according to claim 19, characterized in that, The reagent contains primers and / or probes for the dCAPS or TaqMan PCR method.

21. A method for screening plants containing at least one nutrient selected from iron, zinc, phosphorus, copper, molybdenum, amino acids, ribobandi glycoside D, and ribobandi glycoside M, wherein the genotype of the plant at position 37 of sequence number 1 is C / C, characterized in that, It includes a procedure for determining the expression level of FRO2.

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