A pesticidal composition and its application

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition and its application. It is characterized in that: the pesticide composition is composed of Isocycloseram, Flometoquin, an auxiliary agent and a dispersion medium, wherein the mass ratio of Isocycloseram to Flometoquin is 1:8 to 4:1. The said composition is used for controlling pests such as thrips, Plutella xylostella, Bemisia tabaci, Laodelphax striatellus, Tetranychus cinnabarinus, aphids, Spodoptera litura, etc. on vegetables and fruit trees. Compared with single agents, the composition shows a significant synergistic effect on pests and significantly improves the control effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition and its application. Background Art

[0002] At present, people are facing two major problems. One is the increasing resistance of crop pests and diseases year by year, and the other is the increasingly prominent problem of the safety of agricultural products. Due to the frequent or unreasonable use of pesticides, the resistance of crop pests and diseases has increased year by year, and the control effect is not good; some agricultural products have serious pesticide residues exceeding the standard, which endangers people's physical health. In response, scientific researchers and relevant personnel are actively looking for solutions. On the one hand, new agents with high activity and safety are developed, and on the other hand, reasonable use methods of pesticides are developed. The research and development of new agents include the development of new compounds and the development of compound agents. The development cycle of new compounds is relatively long and requires huge R & D costs, while the development of compound agents has a relatively short time cycle and lower costs. Two or more active ingredients in compound agents often exhibit three interactions, namely synergism, additivity, and antagonism. The applicant has found that the compounding of Isocycloseram and Flometoquin has a synergistic effect in the development of agents.

[0003] Isocycloseram is a new type of isoxazoline insecticide developed by Syngenta, which can control pests and mites such as Helicoverpa assulta, Plutella xylostella, Diabrotica virgifera virgifera, Thrips tabaci, Tetranychus urticae, etc.

[0004] Flometoquin is a new type of quinoline insecticide and acaricide jointly developed by Meiji Seika Pharma Co., Ltd. and Nippon Kayaku Co., Ltd. in Japan. It can be used for crops such as vegetables, fruits, and tea trees to control various pests with small individuals, strong reproductive ability, and high control difficulty, including thrips, aphids, leaf miners, whiteflies, rust mites, silverleaf whiteflies, etc. It acts on the quinone reductase Qi site of the inner mitochondrial membrane, blocks the energy conversion of Flometoquin by inhibiting electron transfer and respiration, and thus kills pests.

[0005] At present, no composition of the compounding of Isocycloseram and Flometoquin has been publicly reported. Summary of the Invention

[0006] The object of the present invention is to provide a pesticide composition with high efficiency, safety, and low residue.

[0007] The technical solution of the present invention is: a pesticide composition and its application, characterized in that: the composition is composed of Isocycloseram, Flometoquin, an adjuvant, and a dispersion medium, wherein the mass ratio of Isocycloseram to Flometoquin is 1:8 to 4:1, and the preferred mass ratio is 1:4.

[0008] The composition can be formulated into a dosage form of suspension concentrate, soluble concentrate, wettable powder, dispersible granule, dispersible oil suspension concentrate, preferably suspension concentrate.

[0009] Furthermore, when the composition is formulated into a suspension concentrate, the auxiliaries contained are selected from one or more of dispersants, wetting agents, antifreezing agents, preservatives, defoaming agents, and thickening agents.

[0010] The composition is used for controlling pests such as thrips, diamondback moths, silverleaf whiteflies, small brown planthoppers, two-spotted spider mites, aphids, and prodenia litura on vegetables and fruit trees.

[0011] The usage method of the composition is spraying.

[0012] The dosage of the active ingredient of the composition is 45 - 90 g / ha.

[0013] The amount of water added to the composition is 450 - 900 kg / ha.

[0014] The composition is sprayed once at the early peak stage of pests, and is used at most once per crop season.

[0015] Beneficial effects of the composition of the present invention: (1) Compared with single agents, the composition shows a significant synergistic effect on pests, significantly improving the control effect; (2) Reducing the dosage of pesticides, reducing the residue of pesticides in agricultural products and the environment, being safe and environmentally friendly; (3) Delaying the resistance of pests. Specific embodiments

[0016] The following further illustrates the present invention in conjunction with examples. The percentages in the examples are all percentage content ratios, but the present invention is not limited thereto.

[0017] Example of indoor combined toxicity determination test

[0018] Test crop: Eggplant, variety: Purple round eggplant.

[0019] Test target: 2nd instar thrips nymphs, variety: Black thrips.

[0020] Pharmaceutical preparation: Using acetone as a solvent, the original drugs of the test agents Isocycloseram and Flometoquin are respectively formulated into original drug mother liquors. On the basis of preliminary tests, the ratios of the two active ingredients are set as 1:16, 1:8, 1:4, 1:1, 4:1, 8:1, 16:1, and the composition mother liquors are respectively prepared for standby. Dilute the above mother liquors with an aqueous solution of 0.1% Tween 80, and design 5 concentrations for each mother liquor.

[0021] Agent treatment: Eggplant seedlings with thrips eggs were cultured in a light incubator (25°C, 16 hours of darkness, 8 hours of light). When the thrips eggs developed into 2nd instar nymphs, the number of nymphs on the leaves was checked to make the number of nymphs on each leaf 25. The marked eggplant leaves were immersed in the above-mentioned liquid medicine for 10 s, taken out and the excess liquid medicine was blotted dry with filter paper. Each treatment was repeated 4 times. Finally, each treatment was placed in a constant temperature light incubator at 25°C for rearing. After 2 days, the survival or death of the larvae was observed. According to the observation results, the mortality rate of each treatment was calculated. Regression analysis was carried out based on the logarithm values of each agent concentration and the corresponding mortality probability values to calculate the LC 50 . According to the Wadley method, the interaction of the mixture was evaluated. Among them, when SR = 0.5 - 1.5, the two agents in the mixture had an additive effect; when SR ≤ 0.5, it was an antagonistic effect; when SR ≥ 1.5, it was a synergistic effect.

[0022] LC 50 (theoretical value) = (a + b) / (a / LC 50 a + b / LC 50 b)

[0023] SR = LC 50 (theoretical value) / LC 50 (actual value)

[0024] The test results are shown in Table 1.

[0025] Table 1 Joint toxicity results of the mixture of Isocycloseram and Flometoquin against thrips nymphs on eggplant

[0026]

[0027] As can be seen from Table 1, after Isocycloseram and Flometoquin were mixed in the test ratio, they showed two different joint effects of addition and synergism against thrips nymphs. When the mass ratio of Isocycloseram and Flometoquin was 1:16, 8:1 - 16:1, the mixed agent showed an additive effect against thrips nymphs. When the mass ratio of Isocycloseram and Flometoquin was 1:8 - 4:1, it showed a synergistic effect against thrips nymphs. Especially when the mass ratio of Isocycloseram and Flometoquin was 1:4, the SR value was 3.96, showing a significant synergistic effect.

[0028] Formulation examples

[0029] Example 1 20% Isocycloseram·Flometoquin suspension concentrate

[0030] Isocycloseram 4%, Flometoquin 16%, polycarboxylate 7.0%, castor oil polyoxyethylene ether 3.0%, polyvinyl alcohol 0.2%, sodium benzoate 0.3%, urea 2.0%, silicone 0.2%, deionized water to make up 100%.

[0031] Example 2 15% Isocycloseram·Flometoquin Suspension Concentrate

[0032] Isocycloseram 12%, Flometoquin 3%, lignosulfonate 6.0%, fatty alcohol polyoxyethylene ether 3.0%, guar gum 0.3%, sodium benzoate 0.3%, glycerol 3.0%, silicone 0.2%, deionized water to make up 100%.

[0033] Examples of Field Efficacy Trials

[0034] Basis for the test: "Pesticide Field Efficacy Test Guidelines Part 6: Insecticides for Controlling Vegetable Thrips" (NY / T 1464.6 - 2007).

[0035] 1. Test Site

[0036] This test was arranged in Yongfa Town, Chengmai County, Hainan Province. The pests in this test plot were severely damaged and the degree of damage was the same. The cultivation conditions in each plot were uniform.

[0037] 2. Test Objects, Crops

[0038] Test object: Thrips, species: Yellow Thrips.

[0039] Test crop: Eggplant, species: Green Long Eggplant.

[0040] 3. Test Design and Arrangement

[0041] 3.1 Test Agents

[0042] The test agent was the 20% Isocycloseram·Flometoquin Suspension Concentrate of Example 1. There were 3 treatment doses, a control agent and a blank control were set. Each single agent in the mixture and the local conventional agent were used as the control agents, and the blank control was the treatment without any agent. Each treatment was set with 4 replicates.

[0043] 3.2 Plot Arrangement

[0044] The plot area was 30 square meters, and the plots were arranged in a randomized block design.

[0045] 4. Application Time, Frequency, Dosage and Water Consumption

[0046] Apply the pesticide at the beginning of the peak occurrence period of thrips, spray once. The application rate of the active ingredient of the test pesticide is 45, 65, 90 g / ha, and the water consumption is 600 kg / ha.

[0047] 5. Investigation time

[0048] Fix and select 15 leaves from the upper part of each plot to investigate the number of insects. Investigate the initial insect population before applying the pesticide, and investigate the number of live insects on the 1st, 3rd, and 7th days after applying the pesticide, and calculate the control effect. The test results are shown in Table 2.

[0049] 6. Calculation of control effect

[0050] PT=(1 - CK0*PT1 / CK1*PT0)*100

[0051] In the formula:

[0052] PT0—the number of insects before applying the pesticide in the pesticide treatment area;

[0053] PT1—the number of insects after applying the pesticide in the pesticide treatment area;

[0054] CK0—the number of insects before applying the pesticide in the blank control area;

[0055] CK1—the number of insects after applying the pesticide in the blank control area.

[0056] Table 2 Field efficacy test results of Example 1 against thrips on eggplants

[0057]

[0058] As can be seen from Table 2, when the application rate of the active ingredient of the 20% Isocycloseram·Flometoquin suspension concentrate prepared by the present invention is 45, 65, 90 g / ha, the field control effects against thrips on eggplants after the 1st, 3rd, and 7th days are all above 83%. The quick-acting property and long-lasting property are good, and they are all higher than the single-agent control pesticide and the conventional control pesticide. Especially when the dosage is 65, 90 g / ha, the control effect is significantly higher than that of the single-agent control pesticide and the conventional control, and the dosage is reduced compared with the single-agent. Through field observation, the treatment pesticide is safe for eggplant plants.

[0059] The above content is a further detailed description of the present invention in combination with specific preferred embodiments. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can still be made, which should all be regarded as belonging to the protection scope of the present invention.

Claims

1. A pesticide composition, characterized in that: The pesticide composition consists of Isocycloseram, Flometoquin, auxiliaries and a dispersion medium, wherein the mass ratio of Isocycloseram to Flometoquin is 1:8 to 4:

1.

2. The composition according to claim 1, wherein The mass ratio of Isocycloseram to Flometoquin is 1:

4.

3. The composition according to claim 1 or 2, wherein The composition can be formulated into a suspension concentrate, a soluble concentrate, a wettable powder, a dispersible granule, a dispersible oil suspension.

4. The composition according to claim 3, wherein The composition can be formulated into a suspension concentrate.

5. A suspending agent containing the composition according to claim 1, characterized in that, The auxiliaries contained are selected from one or more of a dispersant, a wetting agent, an antifreezing agent, a preservative, an antifoaming agent, a thickening agent.

6. Use of the composition according to claim 1, characterized in that, It is used to control thrips, diamondback moth, silverleaf whitefly, small brown planthopper, two-spotted spider mite, aphid, and prodenia litura on vegetables and fruit trees.

7. The application according to claim 6, wherein It is used to control thrips on eggplants.

8. The application according to claim 7, characterized in that The application rate of the active ingredient is 45 to 90 g / ha.

9. The application according to claim 7, wherein The amount of water added is 450 to 900 kg / ha.

10. The application according to claim 7, wherein Spray the medicine once at the early peak stage of thrips on eggplants, and use it at most once per crop season.

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