Bod5 value detection device and method

Abstract

The application provides a BOD5 value detection device, comprising a culture chip module, a detection chip module and a power module; the culture chip module comprises a closed space structure formed by a heat preservation block, a mixing module and a temperature control module arranged in the closed space structure; the detection chip module comprises a cavity structure and a fluorescence sheet arranged at the bottom of the cavity structure; the cavity structure is arranged in the closed space structure and is provided with a detection inlet, a detection outlet and a fiber optic socket corresponding to the fluorescence sheet; the mixing module is connected with the power module through a capillary tube to mix the water sample to be tested; the other end of the mixing module is communicated with the detection inlet to input the mixed water sample to be tested into the detection chip module; the temperature control module is attached to the mixing module and the detection chip module to heat and control the water sample to be tested; when a fiber is inserted into the fiber optic socket, the fiber optic beam of the fiber irradiates the fluorescence sheet from the fiber optic socket to obtain a reflected fluorescence signal, and the BOD value is calculated through a light data converter; the BOD5 value is the difference between the BOD values before and after the water sample to be tested is heated, and rapid detection can be realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection equipment, in particular to a BOD5 value detection device and method. BACKGROUND

[0002] For the existing method of BOD5 detection, the current method is mainly based on the change of dissolved oxygen concentration in water sample before and after a period of time. The commonly used methods are dissolved oxygen electrode method, differential pressure method and microbial membrane method.

[0003] The dissolved oxygen electrode method is the current national standard method, and its principle is to fill the water sample into the culture bottle, and then seal it. The bottle should not be air permeable, and it should be placed in a constant temperature condition for 5 days. Before and after the culture, the dissolved oxygen concentration is measured by the dissolved oxygen electrode. The difference between the two can be used to calculate the mass of oxygen consumed per liter of water, that is, the BOD5 value. However, the dissolved oxygen electrode method requires five days of constant temperature standing time, and the detection time is relatively long. The BOD5 result can be obtained after five days. During the entire detection process, the dissolved oxygen content needs to be detected twice by human, and it needs to be done quickly to prevent changes in dissolved oxygen during the open detection process, which increases many manual operation errors and the influence of indoor environment. The dissolved oxygen electrode is unstable, and the diffusion of dissolved oxygen concentration during the detection process leads to a continuous decrease in the displayed concentration. However, the accurate result needs to be stable, so in general operation, the experimenter selects the relatively stable value, which also increases the personal preference. The detection process requires a professional constant temperature refrigerator to maintain constant temperature for five days, which also hinders the possibility of on-site rapid detection of BOD5;

[0004] Differential pressure method: in a closed culture bottle, the dissolved oxygen in the water sample is consumed by microorganisms, and the microorganisms produce CO2 equivalent to the oxygen consumption due to respiration. When CO2 is absorbed, the pressure of the closed system decreases, and the BOD5 value of the water sample can be calculated according to the pressure drop. However, the differential pressure method also needs five days to see obvious pressure difference; the consistency of the detection result is poor, and there are many factors affecting the result difference, such as water temperature and reaction of other non-oxidizing bacteria in water;

[0005] Microbial membrane method: its principle is that water samples and air enter the flow tank and contact the microbial sensor, and the biodegradable organic pollutants in the water sample are decomposed by the microorganism bacteria fixed on the membrane. When the diffusion rate reaches a constant, the oxygen mass diffused to the surface of the oxygen electrode also reaches a constant and generates a constant current. Since the difference between the current and the biodegradable organic matter in the water sample and the reduction of oxygen are quantitatively related, the five-day biochemical oxygen demand of the water sample can be inferred. However, the microorganisms used in the microbial membrane method are limited, while the microorganisms in nature are diverse, so there will be differences between different water samples and the national standard BOD5 method. The microorganisms are fixed on the membrane, and the storage conditions are relatively strict. The consistency between batches is also a severe test. Different batches and different storage times will affect the results. SUMMARY

[0006] An object of the present application is to provide a BOD5 value detection device and method, aiming to realize rapid detection of BOD5, solve the problems of long detection time, poor detection sensitivity, more human factors interference, and difficult on-site detection.

[0007] To achieve the above object, according to one aspect of the present application, some embodiments of the present application provide a BOD5 value detection device, comprising: a culture chip module, comprising a closed space structure formed by a heat preservation block and a mixing module and a temperature control module arranged in the closed space structure; a detection chip module, comprising a cavity structure and a fluorescence sheet arranged at the bottom of the cavity structure, the cavity structure is arranged in the closed space structure, and is provided with a detection inlet, a detection outlet and a fiber optic socket corresponding to the fluorescence sheet; the mixing module is connected to a power module through a capillary tube to obtain the mixed water sample to be tested, and the other end is communicated with the detection inlet to input the mixed water sample to be tested into the detection chip module; the temperature control module is attached to the mixing module and the detection chip module to heat and control the water sample to be tested; when the optical fiber is inserted into the fiber optic socket, the optical fiber beam of the optical fiber irradiates the fluorescence sheet from the fiber optic socket to obtain a reflected fluorescence signal, and the BOD value is obtained by calculating through a preset light data converter, wherein the BOD5 value is the difference between the BOD value before heating and the BOD value after heating of the water sample to be tested.

[0008] Optionally, the heat preservation block comprises: a first heat preservation block and a second heat preservation block; the first heat preservation block and the second heat preservation block are mutually buckled to form the closed space structure.

[0009] Optionally, the temperature control module comprises: a heating block and a heat transfer block; the heating block is tightly attached to and clamped in the heat transfer block; the heat transfer block is tightly attached to the mixing module and the detection chip module.

[0010] Optionally, the mixing module is a microfluidic chip, and the mixing module is formed into a groove structure with an arcuate curvature, and the water sample to be tested is uniformly mixed by the groove structure and then input into the detection chip module.

[0011] Optionally, the culture chip module further comprises a temperature sensing block, the temperature sensing block is in close contact with the detection chip module and is connected with the heating block to control the start and stop of the heating block.

[0012] Optionally, the detection inlet is arranged at the bottom end of the cavity structure, and the detection outlet and the optical fiber socket are arranged at the top end of the cavity structure, and the center hole line of the optical fiber socket is perpendicular to the fluorescent sheet and located directly above the fluorescent sheet.

[0013] Optionally, the power module comprises a sampling valve and a peristaltic pump, the sampling valve is connected to a container containing the water sample to be tested through a capillary tube, the sampling valve is arranged between the mixing module and the peristaltic pump and is connected through a capillary tube, the peristaltic pump is arranged between the sampling valve and the detection outlet and is connected through a capillary tube, when the sampling valve is in a first state, the peristaltic pump rotates, and the water sample to be tested is pumped into the mixing module through the sampling valve, when the sampling valve is in a second state, the peristaltic pump rotates, and the water sample to be tested is sequentially pumped out through the detection outlet, the peristaltic pump and the sampling valve.

[0014] Optionally, the inner diameter of the groove of the groove structure is 0.1mm to 7.5mm, and the groove curvature is from 10° to 170°.

[0015] Optionally, the heat transfer block comprises a first heat transfer block and a second heat transfer block, the first heat transfer block is attached to the bottom of the closed space structure, the bottom surface of the heating block is in close contact with the first heat transfer block, the top surface of the heating block is in close contact with the second heat transfer block, and the mixing module and the cavity structure of the detection chip module are located above the second heat transfer block and are attached to the second heat transfer block.

[0016] According to another aspect of the present application, the present application also provides a method for detecting the BOD5 value, the method comprising:

[0017] Sampling process: the water sample to be tested is pumped into the mixing module of the culture chip module by the power module for uniform mixing;

[0018] The uniformly mixed water sample to be tested is input into the cavity structure of the detection chip module through the detection inlet, and after the water sample to be tested wets the fluorescent sheet, it gradually overflows upwards until it is discharged from the detection outlet;

[0019] Detection process: the fluorescent sheet is irradiated by the optical fiber beam from the optical fiber socket, and the reflected fluorescent signal is calculated and recorded by the light data converter to obtain the initial dissolved oxygen value BOD.

[0020] Turning on the heating program, heating the water sample to be tested in the cavity structure of the mixing module and the detection chip module and keeping it at a preset constant temperature, after keeping for a predetermined time, again irradiating the fluorescence sheet with the optical fiber beam from the optical fiber socket, and transferring the reflected fluorescence signal to the light data converter for calculation and recording to obtain the final state dissolved oxygen value BOD;

[0021] Calculating the difference between the initial dissolved oxygen value BOD and the final state dissolved oxygen value BOD to obtain the BOD5 value of the water sample to be tested.

[0022] The present application starts with accelerating microbial consumption, and through the heating and stirring method of the temperature control module of the culture chip module to stimulate the activity of microorganisms in water, the detection time is shortened; and through the mixing module (closed groove) of the culture chip module, human factor interference in the two detection processes is eliminated; the detection method used by the system is fluorescence method, the principle of which is that the modulated green light is irradiated on the fluorescent substance to excite it and emit red light, and since oxygen molecules can carry energy (quenching effect), the time and intensity of the excited red light are inversely proportional to the concentration of oxygen molecules. The red light source synchronized with the green light is used as a reference, the phase difference between the excited red light and the reference light is measured, and compared with the internal calibration value, the concentration of oxygen molecules is calculated, and the final value is output after temperature compensation. Using the quenching principle, the maintenance amount is small, no oxygen is consumed, no flow rate and stirring are required, not affected by chemical substances such as sulfides, high resolution and measurement accuracy. Using the assembly, the detection performance is better than that of the dissolved oxygen electrode method. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The structure schematic diagram of the chip module provided for the embodiment of the present application;

[0024] Figure 2 The structure schematic diagram of the detection chip module provided for the embodiment of the present application;

[0025] Figure 3 The structure schematic diagram in the sampling state provided for the embodiment of the present application;

[0026] Figure 4 The structure schematic diagram in the detection state provided for the embodiment of the present application. DETAILED DESCRIPTION

[0027] To make the purposes, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0028] Referring to Figure 1 An embodiment of the present application provides a BOD5 value detection device, comprising:

[0029] The culture chip module 1 comprises a closed space structure formed by the heat preservation block 110 and a mixing module 111 and a temperature control module 112 arranged in the closed space structure.

[0030] As shown in the figure, Figure 2 The detection chip module 2 comprises a cavity structure and a fluorescence sheet 210 arranged at the bottom of the cavity structure, the cavity structure is arranged in the closed space structure, and the cavity structure is provided with a detection inlet 211, a detection outlet 212, and a fiber optic socket 213 corresponding to the fluorescence sheet 210.

[0031] One end of the mixing module 111 is connected to the power module 3 through the capillary tube 7 to obtain the water sample to be tested for mixing, and the other end is communicated with the detection inlet 211 to input the mixed water sample to be tested into the cavity structure of the detection chip module 2.

[0032] The temperature control module 112 is attached to the mixing module 111 and the detection chip module 2 to heat and control the water sample to be tested.

[0033] When the optical fiber is inserted into the fiber optic socket 213, the optical fiber beam of the optical fiber irradiates the fluorescence sheet 210 from the fiber optic socket 213 to obtain a reflected fluorescence signal, and the BOD value is obtained by calculating through the preset light data converter 4, wherein the BOD5 value is the difference between the BOD value before heating and the BOD value after heating of the water sample to be tested.

[0034] Specifically, the heat preservation block 110 comprises a first heat preservation block 1101 and a second heat preservation block 1102.

[0035] The first heat preservation block 1101 and the second heat preservation block 1102 are buckled to each other to form the closed space structure.

[0036] Specifically, the temperature control module 112 comprises a heating block 1121 and a heat transfer block 1122.

[0037] The heating block 1121 is tightly attached to the heat transfer block 1122 and is clamped between the heat transfer block 1122;

[0038] The heat transfer block 1121 is tightly attached to the mixing module 111 and the detection chip module 2, specifically the cavity structure of the detection chip module 2. Optionally, a layer of heat transfer block is arranged between the heat transfer block 1121 and the cavity structure of the chip module 2.

[0039] Specifically, the mixing module 111 is a microfluidic chip and is collectively formed into a groove structure with an arc bending. The water sample to be tested is uniformly mixed through the groove structure and then input into the cavity structure of the detection chip module 2.

[0040] Specifically, the culture chip module 1 further comprises a temperature sensing block 113; the temperature sensing block 113 is tightly attached to the detection chip module 2 and is connected with the heating block 1121 to control the start and stop of the heating block 1121.

[0041] Specifically, the detection inlet 211 is arranged at the bottom end of the cavity structure;

[0042] The detection outlet 212 and the optical fiber socket 213 are arranged at the top end of the cavity structure respectively, and the center hole line of the optical fiber socket 213 is perpendicular to the fluorescence sheet 210 and is located directly above the fluorescence sheet 210.

[0043] Specifically, the power module 3 comprises a sampling valve 310 and a peristaltic pump 311;

[0044] The sampling valve 310 is connected to a container containing the water sample to be tested through a capillary tube;

[0045] The sampling valve 310 is arranged between the mixing module 111 and the peristaltic pump 311 and is connected through a capillary tube;

[0046] The peristaltic pump 311 is arranged between the sampling valve 310 and the detection outlet 212 and is connected through a capillary tube;

[0047] As shown in Figure 3 When the sampling valve 310 is in the first state, the peristaltic pump 311 rotates, and the water sample to be tested is pumped into the mixing module 111 through the sampling valve 310;

[0048] As shown in Figure 4 When the sampling valve 310 is in the second state, the peristaltic pump 311 rotates, and the water sample to be tested is sequentially pumped out through the detection outlet 212, the peristaltic pump 311 and the sampling valve 310.

[0049] Specifically, the groove inner diameter of the groove structure is 0.1mm to 7.5mm, and the groove curvature is from 10° to 170°.

[0050] Specifically, the heat transfer block 1122 includes a first heat transfer block and a second heat transfer block.

[0051] The first heat transfer block is attached to the bottom of the closed space structure, the bottom surface of the heating block 1121 is tightly attached to the first heat block, and the top surface of the heating block 1121 is tightly attached to the second heat transfer block.

[0052] The mixing module 111 and the cavity structure of the detection chip module 2 are located above and attached to the second heat transfer block.

[0053] As a specific embodiment, the BOD 5 detection device in the application is composed of a chip device and a power module. The chip device, as shown in Figure 1 The power module 3 includes a sample injection valve 310 and a peristaltic pump 311; the peristaltic pump 311 is the power part of the detection system, and the sample injection valve 310 is responsible for switching the sample injection state and the detection operation state.

[0054] The culture chip module 1 is responsible for uniformly mixing all substances entering the chip and rapidly heating, constant temperature and sensing real-time temperature; the culture chip module 1 includes a heating block, a heat transfer block, a heat preservation block, a temperature sensing block and a mixing module; the heat preservation block is divided into an upper heat preservation block (first heat preservation block) and a lower heat preservation block (second heat preservation block), which together wrap the heating block, heat transfer block, temperature sensing block and mixing module; the heating block is tightly attached to the heat transfer block; the heating block uses metal wires or heating film to provide heat source; the heat transfer block is tightly attached to the mixing module; the heat transfer block is made of metal material to ensure uniform heat dissipation; the temperature sensing block is located directly above or below the detection chip module and is tightly attached to the detection chip module; the detection chip module is mainly responsible for signal detection of dissolved oxygen content in water sample; the detection chip module includes a detection inlet, a detection outlet, a fluorescent sheet and a fiber optic socket; the detection inlet is at the bottom end of the detection chip; the fluorescent sheet is attached to the bottom of the detection chip cavity; the detection outlet is located directly above the detection chip cavity, and the top end of the cavity is an arc surface; the hole line of the fiber optic socket is perpendicular to the fluorescent sheet and is located directly above the fluorescent sheet. The culture chip module and the detection chip module can be on one set of chip or can be two separate chips; the capillary tube 5 serves as the connecting pipeline of the sample injection valve, peristaltic pump, culture chip module and detection chip module.

[0055] The detection process is to switch the sampling valve to the sampling state (first state), the peristaltic pump rotates to draw the water sample 6, the water sample 6 enters the mixing module, and then enters the cavity of the detection chip from the detection inlet, the water sample 6 gradually fills from the bottom layer of the cavity of the detection chip to the top, and finally overflows from the detection outlet; the water sample 6 finally overflows out of the system from the sampling valve, and the sampling valve is switched to the detection running state (second state); the dissolved oxygen content is detected at this time, that is, the optical fiber beam irradiates the fluorescent sheet, and the fluorescence intensity is collected, and the initial dissolved oxygen content is calculated by the light data converter 4. Start heating, the temperature sensing block detects the temperature in real time, and controls the start and stop of the heating block; the heat preservation block avoids heat dissipation to ensure constant temperature; the peristaltic pump continues to run, and the dissolved oxygen content at this time is collected after 4-8 hours, that is, the finished state dissolved oxygen content, and the BOD 5 value is calculated according to the formula by subtracting the two dissolved oxygen contents. Clean the chip and detect the technology.

[0056] According to an aspect of the present application, the present application also provides a method for detecting BOD5 value using the BOD5 value detection device, the method comprising:

[0057] The sampling process: the water sample to be tested is pumped into the mixing module of the culture chip module by the power module for uniform mixing;

[0058] The uniformly mixed water sample to be tested is input into the cavity structure of the detection chip module through the detection inlet, and the water sample to be tested gradually overflows upwards after wetting the fluorescent sheet until it is discharged from the detection outlet;

[0059] The detection process: the fluorescent sheet is irradiated by the optical fiber beam from the optical fiber socket, and the reflected fluorescent signal is calculated and recorded by the light data converter, and the initial dissolved oxygen value BOD is obtained;

[0060] Start the heating program, heat the water sample to be tested in the mixing module and the cavity structure of the detection chip module and keep it at a preset constant temperature, and then use the optical fiber beam from the optical fiber socket to irradiate the fluorescent sheet again, and calculate and record the reflected fluorescent signal by the light data converter to obtain the final state dissolved oxygen value BOD;

[0061] The difference between the initial dissolved oxygen value BOD and the final state dissolved oxygen value BOD is calculated to obtain the BOD5 value of the water sample to be tested.

[0062] The use process of the system is illustrated by a case:

[0063] The sampling process: after the water sample to be tested is prepared, the sampling valve is switched to the sampling state, the peristaltic pump rotates to draw the water sample, the water sample enters the groove of the mixing module of the culture chip through the capillary, the water sample gradually fills the groove of the mixing module and expels the original air, until the water sample enters the detection cavity from the detection inlet, the water sample gradually overflows upwards after wetting the fluorescent sheet, until it is discharged from the detection outlet, at this time the water sample is discharged out of the system through the peristaltic pump and the sampling valve.

[0064] Detection process: the peristaltic pump continues to rotate, the sample valve is switched to the detection state, the optical fiber beam irradiates the fluorescent sheet from the optical fiber socket, and the reflected fluorescent signal is transmitted to the light data converter for calculation and recording, at this time the initial dissolved oxygen value (BOD initial) is obtained; the chip module starts heating according to the predetermined program, the heating block starts to run, the heat emitted is uniformly transmitted to the culture chip and the detection chip by the heat transfer block, and the heat continuously emitted is wrapped and isolated by the upper and lower heat preservation blocks to maintain the system temperature constant. After heating for 4-8 hours, the optical fiber beam irradiates the fluorescent sheet from the optical fiber socket again, and the reflected fluorescent signal is transmitted to the light data converter for calculation and recording, at this time the final dissolved oxygen value (BOD final) is obtained, and the BOD5 value of the water sample is obtained by the difference between BOD final and BOD initial; clean the chip and detect the technology.

[0065] The microfluidic chip is used as a BOD5 detection platform; the flat structure of the microfluidic chip is very conducive to rapid and uniform heating.

[0066] The unique groove design adopts an arc bending design for all grooves, avoiding the hiding of dead angle bubbles and ensuring uniform stirring of the water body in the flow path;

[0067] The fluorescence marker is used to determine the dissolved oxygen content; the fluorescence method has strong sensitivity and can accurately realize the characterization of the dissolved oxygen content;

[0068] The detection water body is heated at a constant temperature; the bottom rapid heating mode and the 360° omnidirectional wrapped constant temperature insulation design ensure the temperature constancy of the system.

[0069] The microfluidic chip is used as a water sample flow path, which can use less water sample, and the entire detection process is in a closed environment, which ensures that the influence of the external environment on the result is minimized;

[0070] The material of the microfluidic chip can be polymethyl methacrylate (PMMA), polydimethylsiloxane (PDMS), epoxy resin, and glass;

[0071] The unique groove design of the present application ensures uniform stirring of the water body, no bubbles are left during sampling, the internal dissolved oxygen is uniform, the microbial activity is excited, the groove inner diameter is 0.1mm to 7.5mm, the total length of the groove is increased or decreased according to actual needs, the groove arc is from 10° to 170°, the number of arc grooves is not limited and can be increased or decreased as needed;

[0072] The fluorescence marker is used to quantify the dissolved oxygen result, the fluorescence detection method has high sensitivity and is very suitable for low content detection; the fluorescence marker is an indole substance, which has high sensitivity to dissolved oxygen;

[0073] The application can detect water constant heating, so that microorganisms can be quickly decomposed at the optimum temperature. The optimum temperature range is 13-80℃.

[0074] Advantages of the application:

[0075] The detection process is maximally referenced to the national standard detection method, and the detection principle is reliable;

[0076] The microfluidic chip is used as the pipeline carrier of the detection process, so that less water sample, higher heating efficiency and more easily controlled operation platform can be used;

[0077] The unique groove design makes the microorganisms always in a passive active state, and the higher activity ensures the maximum consistency of dissolved oxygen on the longitudinal section of the pipeline, and accurately identifies the dissolved oxygen content in water;

[0078] The fluorescence marker is used to replace the dissolved oxygen electrode to represent the dissolved oxygen content in water. The fluorescence substance can be converted into a more obvious electric signal through multi-stage amplification of weak light signals, so as to obtain extremely high sensitivity;

[0079] In the detection process, the optimum temperature of the microorganisms is obtained by heating to maintain the optimum biological activity, so as to more quickly degrade the pollutants and reach the peak point of their own metabolism;

[0080] In summary, the starting point of the application is to use a small amount of water sample, and to combine the flat microfluidic chip groove, the diaphragm heating and constant temperature insulation, so as to realize the accurate temperature control of the system and ensure the optimum microbial degradation temperature. The unique groove design realizes the replacement of manual stirring of the water sample, ensures the consistency of dissolved oxygen and the activity of microorganisms. Finally, under the comprehensive action of heating, stirring and small water body, the BOD5 can be quickly measured.

[0081] It is obvious for those skilled in the art that the application is not limited to the details of the above exemplary embodiments, and the application can be implemented in other specific forms without departing from the spirit or essential characteristics of the application. Therefore, the embodiments should be regarded as exemplary and non-limiting, and the scope of the application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the application. Any reference signs in the claims should not be regarded as limiting the claims. In addition, the terms used in the embodiments of the application are only for the purpose of describing the specific embodiments, and are not intended to limit the application.

[0082] It should be noted that the terms "first", "second" and the like in the description and in the claims of the present application are used for distinguishing between similar objects and not necessarily for describing a specific sequential or chronological order. It is to be understood that the data used in the above description and the claims is presented only by way of example and that other data, including data that is not explicitly mentioned, can be used in the same manner. The use of the terms "first", "second", "and the like is mainly used for distinguishing between two distinct sets expressed by these terms and can pose no order between the great sets expressed by these terms.

[0083] Furthermore, the terms "comprise", "comprising", "have", "having", "include", "including", "contain", "containing", "provide", "providing", and the like, are used in the detailed description and in the claims of this application and are intended to be construed as inclusive or open-ended, that is, indicating the presence of what is recited, but not excluding or in any way limiting the presence of other recited, unstated, or inherent features.

[0084] In the description of the present application, unless otherwise specified, " / " means that the objects associated in front and back are in a "or" relationship, for example, A / B can represent A or B; "and / or" in the present application is only a description of the association of the associated objects, which means that there can be three relationships, for example, A and / or B, which can represent: A exists alone, A and B exist together, and B exists alone, where A, B can be singular or plural. Depending on the context, the word "if" or "if" as used herein can be interpreted as "when" or "when" or "in response to determining" or "in response to detecting". Similarly, depending on the context, the phrase "if determined" or "if detected (stated condition or event)" can be interpreted as "when determined" or "in response to determining" or "when detected (stated condition or event)" or "in response to detecting (stated condition or event)".

[0085] The above, the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A BOD5 value detection device, characterized in that, include: A chip cultivation module includes a closed space structure formed by an insulation block and a hybrid module and a temperature control module disposed within the closed space structure; The detection chip module includes a cavity structure and a fluorescent sheet disposed at the bottom of the cavity structure. The cavity structure is placed in the closed space structure and is provided with a detection inlet, a detection outlet and an optical fiber connector corresponding to the fluorescent sheet. The mixing module obtains the water sample to be tested and mixes it by connecting an external power module through a capillary tube, and the other end is connected to the detection inlet to input the mixed water sample to be tested into the detection chip module. The temperature control module is attached to the mixing module and the detection chip module to heat and control the temperature of the water sample to be tested; When an optical fiber is inserted into the optical fiber socket, the optical fiber beam shines on the fluorescent sheet from the optical fiber socket to obtain a reflected fluorescence signal, and the BOD value is calculated by a preset optical data converter. The BOD5 value is the difference between the BOD value of the water sample before heating and the BOD value after heating. The insulation block includes a first insulation block and a second insulation block; the first insulation block and the second insulation block are interlocked to form the closed space structure. The temperature control module includes a heating block and a heat transfer block; the heating block is in close contact with the heat transfer block and is sandwiched within the heat transfer block; the heat transfer block is in close contact with the mixing module and the detection chip module. The culture chip module further includes a temperature sensing block; the temperature sensing block is in close contact with the detection chip module and is connected to the heating block to control the start and stop of the heating block; The hybrid module is a microfluidic chip, which is assembled to form a groove structure with an arc-shaped bend. The water sample to be tested is uniformly mixed through the groove structure and then input into the detection chip module.

2. The BOD5 value detection device according to claim 1, characterized in that, The detection inlet is located at the bottom end of the cavity structure; The detection outlet and the optical fiber connector are respectively located at the top of the cavity structure, and the center hole line of the optical fiber connector is perpendicular to the fluorescent sheet and located directly above the fluorescent sheet.

3. The BOD5 value detection device according to claim 1, characterized in that, The power module includes: a sample injection valve and a peristaltic pump; The injection valve is connected to a container holding the water sample to be tested via a capillary tube. The injection valve is located between the mixing module and the peristaltic pump, and is connected via a capillary tube; The peristaltic pump is disposed between the injection valve and the detection outlet, and is connected to the outlet via a capillary tube; When the sampling valve is in the first state, the peristaltic pump rotates, and the water sample to be tested is pumped into the mixing module through the sampling valve. When the sampling valve is in the second state, the peristaltic pump rotates, pumping the water sample to be tested sequentially through the detection outlet, the peristaltic pump, and the sampling valve.

4. The BOD5 value detection device according to claim 1, characterized in that, The groove structure has an inner diameter of 0.1 mm to 7.5 mm and a groove curvature of 10° to 170°.

5. The BOD5 value detection device according to claim 1, characterized in that, The heat transfer block includes a first heat transfer block and a second heat transfer block; The first heat transfer block is attached to the bottom of the closed space structure, the bottom surface of the heating block is in close contact with the first heat transfer block, and the top surface of the heating block is in close contact with the second heat transfer block; The cavity structure of the hybrid module and the detection chip module is located on the second heat transfer block and is attached to the second heat transfer block.

6. A method for detecting BOD5 value using the BOD5 value detection device according to any one of claims 1 to 5, characterized in that, The method includes: Sampling process: The water sample to be tested is pumped into the mixing module of the culture chip module through the power module for uniform mixing; The uniformly mixed water sample is input into the cavity structure of the detection chip module through the detection inlet. After the water sample wets the fluorescent sheet, it gradually overflows upwards until it is discharged from the detection outlet. Detection process: A fluorescent sheet is illuminated through an optical fiber socket by an optical fiber beam, and the reflected fluorescence signal is sent to an optical data converter for calculation and recording to obtain the initial dissolved oxygen (BOD) value. The heating program is started to heat the water sample to be tested in the cavity structure of the mixing module and the detection chip module and maintain it at a preset constant temperature. After maintaining it for a predetermined time, the fluorescent sheet is irradiated again with an optical fiber beam from the optical fiber port, and the reflected fluorescence signal is handed over to the optical data converter for calculation and recording to obtain the final dissolved oxygen value (BOD). The difference between the initial dissolved oxygen value (BOD) and the final dissolved oxygen value (BOD) is calculated to obtain the BOD5 value of the water sample to be tested.

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