Waste liquid needle installation mechanism, waste liquid needle assembly and immunoblotting detection device

Abstract

The present invention provides a waste liquid needle mounting mechanism, a waste liquid needle assembly and an immunoblotting detection device, wherein the waste liquid needle mounting assembly includes a mounting base and a positioning assembly; wherein the positioning assembly includes: a block connected to the mounting base, and provided with a plurality of first through holes extending through along a first direction, the plurality of first through holes being spaced apart along a second direction, and the second direction being perpendicular to the first direction; a plurality of mounting blocks arranged in a one-to-one correspondence with the plurality of first through holes; each mounting block is partially inserted into the corresponding first through hole and can move relative to the block along the first direction; a plurality of elastic members, each elastic member being arranged between a mounting block and the block, and the elastic member expanding and contracting with the movement of the mounting block. Each mounting block is used to connect to a waste liquid needle. When the waste liquid needle mounting mechanism is used, it can ensure that the plurality of waste liquid needles can all contact the membrane strip body of the corresponding immunoblotting detection membrane strip and have a better liquid removal effect.

Description

Technical Field

[0001] The invention belongs to the technical field of immunoassay and relates to a waste liquid needle installation mechanism, a waste liquid needle assembly and an immunoblotting detection device. Background Art

[0002] Immunoblotting, also known as protein blotting, is a method for monitoring a certain protein in a complex sample based on the specific binding of antigens and antibodies. It mainly includes the steps of gel electrophoresis of protein samples, membrane transfer, elution, blocking, elution with blocking solution, incubation with primary antibody, elution with primary antibody, incubation with secondary antibody, elution with secondary antibody, and color development. Many of the steps require the use of a waste liquid needle to remove liquid during execution. For example, before membrane transfer, the PVDF membrane on the immunoblotting detection membrane strip needs to be activated by soaking in an organic solvent such as ethanol, and then the organic solvent needs to be removed; detergents are used to wash the immunoblotting detection membrane strip in the steps of membrane transfer, elution, and color development, and the washing solution needs to be removed after each wash. In other words, using a waste liquid needle to remove liquid is an essential operation in the analysis process of the immunoblotting detection membrane strip.

[0003] Immunoblotting membrane strips generally include a housing and a membrane strip body disposed inside the housing. The housing is provided with a through-groove, and the membrane strip body is exposed at the through-groove, and a cavity for accommodating liquid is formed at the through-groove. In practice, liquid accumulates in the cavity. In other words, the liquid in the cavity is removed using the waste liquid needle. Therefore, when using the waste liquid needle to remove liquid from the immunoblotting membrane strip, the waste liquid needle is required to extend into the cavity and contact the membrane strip body to achieve a better liquid removal effect.

[0004] Currently, in order to improve the processing efficiency of immunoblotting analysis, multiple waste liquid needles are usually used to remove liquid from multiple immunoblotting membrane strips simultaneously. However, a problem exists: in the existing technology, the waste liquid needles are mostly rigidly fixed by tightening screws or clamps, and then the position of the waste liquid needles is determined by manual visual inspection and manual adjustment. When multiple waste liquid needles are used to process multiple immunoblotting membrane strips at the same time, such a fixing and adjustment method makes it difficult to ensure that each waste liquid needle can extend into the corresponding immunoblotting membrane strip cavity and contact the membrane strip body. Summary of the Invention

[0005] The purpose of the present invention is to provide a waste liquid needle installation mechanism, a waste liquid needle assembly and an immunoblotting detection device to ensure that each waste liquid needle can contact the corresponding immunoblotting detection membrane strip when using multiple waste liquid needles to simultaneously remove liquid from multiple immunoblotting detection membrane strips.

[0006] To achieve the above-mentioned object, the present invention provides a waste liquid needle mounting mechanism, comprising a mounting base and a positioning assembly; wherein the positioning assembly comprises:

[0007] a stopper connected to the mounting substrate, and provided with a plurality of first through holes extending along a first direction, wherein the plurality of first through holes are arranged at intervals along a second direction, wherein the second direction is perpendicular to the first direction;

[0008] a plurality of mounting blocks arranged in one-to-one correspondence with the plurality of first through holes; each of the mounting blocks is partially disposed in the corresponding first through hole and is movable relative to the stop block along the first direction; and

[0009] A plurality of elastic members, each of which is disposed between one of the mounting blocks and the stop block, and the elastic member expands and contracts as the corresponding mounting block moves.

[0010] Optionally, the mounting block includes a base and a coupling column, the coupling column is connected to one end of the base facing the stop block, and the coupling column is partially inserted into the corresponding first through hole; the elastic member is sleeved on the coupling column, one end of the elastic member contacts the stop block, and the other end of the elastic member contacts the base.

[0011] Optionally, a plurality of annular grooves are provided on the surface of the stopper facing the elastic member, and each of the annular grooves is arranged around one of the first through holes; and the end of the elastic member in contact with the stopper is accommodated in the corresponding annular groove.

[0012] Optionally, the mounting substrate includes a first plate body and a second plate body connected to each other, and the first plate body and the second plate body are perpendicular to each other;

[0013] The first plate is connected to the stopper, and the second plate is located on a side of the mounting block away from the stopper. The second plate is used to contact the surface of the mounting block away from the stopper and prevent the mounting block from separating from the corresponding first through hole.

[0014] Optionally, the waste liquid needle mounting mechanism also includes a plurality of guide limit members, each of the guide limit members including a first limit portion and a second limit portion, the first limit portion including a protrusion arranged on the end face of the mounting block away from the stop block, and the protrusion extends along the first direction; the second limit portion includes a third through hole provided on the second plate body and extending through the first direction, and the third through hole is used for the protrusion to pass through.

[0015] Optionally, the sum of the natural length of the elastic member and the length of the base is greater than the distance from the point where the stop block contacts the elastic member to the surface of the second plate facing the stop block; the length of the elastic member refers to the size of the elastic member in the first direction, and the length of the base refers to the size of the base in the first direction.

[0016] Optionally, the mounting block is provided with a joining channel extending through along the first direction, and the joining channel is used for the waste liquid needle to pass through; the second plate body is provided with a second through hole extending through along the first direction, the second through hole is connected to the joining channel, and is used for the waste liquid needle to pass through.

[0017] To achieve the above objectives, the present invention also provides a waste liquid needle assembly, comprising multiple waste liquid needles and the waste liquid needle mounting mechanism as described above, all of the waste liquid needles extend along the first direction, and each of the waste liquid needles is connected to one of the mounting blocks.

[0018] Optionally, a liquid suction port is formed at the end of the waste liquid needle away from the stopper, and an air hole is further provided on the side wall of the waste liquid needle, the air hole is adjacent to the liquid suction port, and the air hole is communicated with the liquid suction port.

[0019] To achieve the above-mentioned purpose, the present invention also provides an immunoblotting detection device, comprising: a shaking table, a driving mechanism and a waste liquid needle assembly as described above; the shaking table is used to carry a carrier plate, the carrier plate is used to carry multiple immunoblotting detection membrane strips, and the multiple immunoblotting detection membrane strips are arranged along the second direction; the waste liquid needle assembly and the carrier plate are arranged along the first direction so that the liquid aspiration port of the waste liquid needle faces the carrier plate; the driving mechanism is connected to the mounting base plate of the waste liquid needle assembly, and is used to drive the waste liquid needle assembly to move along the first direction so that the liquid aspiration port of the waste liquid needle is close to or away from the immunoblotting detection membrane strip.

[0020] Compared with the prior art, the waste liquid needle installation mechanism, waste liquid needle assembly and immunoblotting detection device of the present invention have the following advantages:

[0021] The aforementioned waste liquid needle mounting mechanism includes a mounting base and a positioning assembly, the positioning assembly includes a stopper, multiple mounting blocks and multiple elastic members; the stopper is connected to the mounting base, and the stopper is provided with multiple first through holes extending through along a first direction, and the multiple first through holes are arranged at intervals along a second direction, and the second direction is perpendicular to the first direction; the multiple mounting blocks are arranged one-to-one with the multiple first through holes; each mounting block is partially inserted into the corresponding first through hole and can move along the first direction; each elastic member is arranged between one mounting block and the stopper, and expands and contracts with the movement of the corresponding mounting block. During application, waste liquid needles are installed on at least part of the mounting blocks, and the waste liquid needles extend along the first direction. In this way, when multiple waste liquid needles are used to absorb liquid on multiple immunoblot detection membrane strips placed on a carrier plate, the entire waste liquid needle mounting mechanism can be controlled to move toward the carrier plate along the first direction so that all waste liquid needles are in contact with the corresponding membrane strip body of the immunoblot detection membrane strip. In this process, due to installation errors or height differences between multiple immunoblot detection membrane strips, there is a sequence when multiple waste liquid needles contact the corresponding immunoblot detection membrane strip. For the waste liquid needle that contacts the immunoblot detection membrane strip first, after it contacts the corresponding membrane strip body of the immunoblot detection membrane strip, as the waste liquid needle mounting mechanism continues to move, it will push the corresponding mounting block to move in the direction away from the carrier plate and compress the corresponding elastic member to avoid rigid interference with the movement of the waste liquid needle mounting mechanism, thereby allowing other waste liquid needles to contact the corresponding membrane strip body of the immunoblot detection membrane strip. That is to say, when the waste liquid needle installation mechanism is used, it can effectively ensure that the multiple waste liquid needles installed thereon can fully contact the corresponding immunoblotting detection membrane strips. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings are provided for a better understanding of the present invention and are not intended to limit the present invention.

[0023] Figure 1 1 is a schematic structural diagram of a waste liquid needle assembly provided according to one embodiment of the present invention;

[0024] Figure 2 1 is a schematic diagram of an application scenario of a waste liquid needle assembly provided according to one embodiment of the present invention, wherein a shaking table, a sample carrier, and a driving mechanism are not shown in the figure;

[0025] Figure 3 1 is a schematic structural diagram of a waste liquid needle of a waste liquid needle assembly provided according to one embodiment of the present invention;

[0026] Figure 41 is a schematic structural diagram of a waste liquid needle of a waste liquid needle assembly provided according to one embodiment of the present invention. Figure 4 Observation direction and Figure 3 different.

[0027] [The following are the descriptions of the reference numerals]:

[0028] 1-waste liquid needle assembly, 10-waste liquid needle mounting mechanism, 100-mounting substrate, 110-first plate body, 120-second plate body, 200-positioning assembly, 210-stop block, 211-first through hole, 220-mounting block, 221-base, 222-engaging column, 230-elastic member, 300-linear guide rail assembly, 411-first limiting portion, 20-waste liquid needle, 30-immunoblotting detection membrane strip, 31-concave cavity. DETAILED DESCRIPTION

[0029] The following describes the embodiments of the present invention through specific specific examples. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the illustrations provided in this embodiment only illustrate the basic concept of the present invention in a schematic manner, and the drawings only show components related to the present invention rather than being drawn according to the number, shape and size of components during actual implementation. During actual implementation, the type, quantity and proportion of each component can be changed at will, and the component layout type may also be more complicated.

[0030] In addition, each embodiment described below has one or more technical features. However, this does not mean that users of the present invention must implement all technical features in any embodiment at the same time, or that they can only implement some or all technical features in different embodiments separately. In other words, as long as implementation is possible, those skilled in the art can, based on the disclosure of the present invention and depending on design specifications or implementation requirements, selectively implement some or all technical features in any embodiment, or selectively implement a combination of some or all technical features in multiple embodiments, thereby increasing the flexibility of the implementation of the present invention.

[0031] As used in this specification, the singular forms "a", "an", and "the" include plural objects, and the plural form "a plurality" includes more than two objects, unless the content clearly indicates otherwise. As used in this specification, the term "or" is generally used in a sense that includes "and / or", unless the content clearly indicates otherwise, and the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection. It can be a mechanical connection or an electrical connection. It can be directly connected or indirectly connected through an intermediate medium, and it can be internal communication between two elements or an interactive relationship between two elements. Relational terms such as "first", "second", etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations, nor do they indicate or imply relative importance or implicitly indicate the number of technical features indicated. It should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "up," "down," "front," "back," "left," "right," "vertical," "horizontal," "top," "bottom," "inside," "outside," "axial," "radial," "circumferential," and the like, indicating positions or location relationships, are based on the positions or location relationships shown in the accompanying drawings and are intended only to facilitate description and simplify the description of the present invention. They do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be construed as limiting the present invention. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0032] The purpose of the present invention is to provide a waste liquid needle installation mechanism, a waste liquid needle assembly and an immunoblotting detection device, aiming to ensure that when multiple waste liquid needles are used to simultaneously remove liquid from multiple immunoblotting detection membrane strips, each of the waste liquid needles can contact the corresponding immunoblotting detection membrane strip to obtain a better liquid removal effect.

[0033] To make the objects, advantages, and features of the present invention more apparent, the present invention is further described below in detail with reference to the accompanying drawings. It should be noted that the drawings are greatly simplified and not to exact scale, and are intended solely to facilitate and clearly illustrate the embodiments of the present invention. The same or similar reference numerals in the drawings represent the same or similar components.

[0034] Figure 1 A schematic structural diagram of a waste liquid needle assembly 1 provided in one embodiment of the present invention is shown. Figure 2A schematic diagram of an application scenario of a waste liquid needle assembly 1 provided in one embodiment of the present invention is shown. The waste liquid needle assembly 1 includes a waste liquid needle mounting mechanism 10 and a plurality of waste liquid needles 20 , and the plurality of waste liquid needles 20 are connected to the waste liquid needle 10 .

[0035] Specifically, please refer to Figure 1 and Figure 2 The waste liquid needle mounting mechanism 10 includes a mounting substrate 100 and a positioning assembly 200. The positioning assembly 200 includes a stopper 210, a plurality of mounting blocks 220, and a plurality of elastic members 230. The stopper 210 is connected to the mounting substrate 100, and is provided with a plurality of first through holes 211 extending through the stopper 210 in a first direction. The plurality of first through holes 211 are arranged at intervals in a second direction. The second direction is perpendicular to the first direction. The plurality of mounting blocks 220 are arranged in a one-to-one correspondence with the plurality of first through holes 211. Each mounting block 220 is partially inserted into the corresponding first through hole 211 and is movable relative to the stopper 210 in the first direction. The number of elastic members 230 is the same as the number of mounting blocks 220, and an elastic member 230 is provided between each mounting block 220 and the stopper 210. The elastic member 230 expands and contracts as the corresponding mounting block 220 moves. Each of the waste liquid needles 20 is mounted on one of the mounting blocks 220 , and the waste liquid needles 20 extend along the first direction.

[0036] In practice, the first direction is generally vertical, and the mounting block 220 is partially located below the stop block 210. When using the waste needle assembly 1 to remove liquid from multiple immunoblot test membrane strips 30, each immunoblot test membrane strip 30 is placed below the corresponding waste needle 20, allowing each waste needle 20 to extend into the corresponding cavity 31 on the immunoblot test membrane strip 30 and contact the membrane strip body of the immunoblot test membrane strip 30. In this way, the waste needle 20 can effectively remove liquid from the immunoblot test membrane strip 30 without causing damage to the waste needle 20.

[0037] Taking the first direction as the vertical direction, the number of the mounting blocks 220 is two, and each of the mounting blocks 220 is equipped with a waste liquid needle 20, and the number of the immunoblotting detection membrane strips 30 to be removed from the liquid is two as an example for detailed description. Hereinafter, the two waste liquid needles 20 are referred to as the first waste liquid needle (not marked in the figure) and the second waste liquid needle (not marked in the figure), and the two immunoblotting detection membrane strips 30 are referred to as the first immunoblotting detection membrane strip (not marked in the figure) and the second immunoblotting detection membrane strip (not marked in the figure). The first waste liquid needle is used to remove the liquid on the first immunoblotting detection membrane strip, and the second waste liquid needle is used to remove the liquid on the second immunoblotting detection membrane strip.

[0038] Two of the immunoblot detection film strips 30 are carried on a loading plate (not shown), and each of the immunoblot detection film strips 30 extends along a third direction, and the two immunoblot detection film strips 30 are arranged along the second direction, and the third direction is perpendicular to the second direction and the first direction. The loading plate is located below the waste liquid needle 20. When the waste liquid needle assembly 1 is used, the mounting base 100 is connected to a driving mechanism (not shown) and can move in a vertical direction under the driving of the driving mechanism.

[0039] When it is not necessary to remove the liquid on the immunoblot detection membrane strip 30, the two waste liquid needles 20 are located above the two immunoblot detection membrane strips 30. After determining that the liquid on the immunoblot detection membrane strip 30 needs to be removed, the waste liquid needle assembly 1 is first driven by the driving mechanism to move downward as a whole until the two waste liquid needles 20 are respectively inserted into the corresponding concave cavities 31 of the immunoblot detection membrane strip 30 and contact the membrane strip body. Then, the two waste liquid needles 20 are used to respectively aspirate the liquid on the corresponding immunoblot detection membrane strip 30 to remove the liquid on the immunoblot detection membrane strip 30. After the pipetting is completed, the waste liquid needle assembly 1 is driven by the driving mechanism to move upward as a whole so that the two waste liquid needles 20 are both separated from the corresponding concave cavities 31 of the immunoblot detection membrane strip 30.

[0040] In the process of making the two waste liquid needles 20 contact with the membrane strip body of the corresponding immunoblot detection membrane strip 30, due to the height difference between the two waste liquid needles 20 due to installation error, or the height difference between the two immunoblot detection membrane strips 30, one of the waste liquid needles 20, for example, the first waste liquid needle, first extends into the concave cavity 31 of the corresponding immunoblot detection membrane strip 30 (i.e., the first immunoblot detection membrane strip) and contacts the membrane strip body, while the other waste liquid needle 20 (i.e., the second waste liquid needle) has not yet contacted the membrane strip body of the corresponding immunoblot detection membrane strip 30 (i.e., the second immunoblot detection membrane strip). For the first waste liquid needle, after it contacts the membrane strip body of the first immunoblot detection membrane strip, as the waste liquid needle mounting mechanism 10 moves downward as a whole, the first waste liquid needle pushes the corresponding mounting block 220 to move upward and compresses the corresponding elastic member 230. While utilizing the elastic member 230 to effectively, stably and fully contact the membrane strip body of the first immunoblot detection membrane strip, it also avoids rigid interference with the downward movement of the waste liquid needle mounting mechanism 10, thereby allowing the second waste liquid needle to continue to move downward until it is in full contact with the membrane strip body of the second immunoblot detection membrane strip. In other words, the configuration of the waste liquid needle mounting mechanism 10 can automatically compensate for the installation height error between multiple waste liquid needles 20 or the height deviation between multiple immunoblot detection membrane strips 30, so that all of the waste liquid needles 20 are in contact with the membrane strip body of the corresponding immunoblot detection membrane strip 30. In addition, the configuration of the waste liquid needle installation mechanism 10 can also buffer the waste liquid needle 20 when the waste liquid needle 20 contacts the corresponding membrane strip body, thereby preventing the waste liquid needle 20 from being damaged by the instantaneous impact force during contact.

[0041] Next, the waste liquid needle assembly 1 is further described. It should be noted that the following only describes a preferred embodiment, which is not a necessary structure and should not constitute an undue limitation on the present invention. In addition, the vertical direction is used as the first direction in the following description.

[0042] Please continue to refer to Figure 1 The mounting substrate 100 includes a first plate 110 and a second plate 120 connected to each other. The first plate 110 and the second plate 120 are perpendicular to each other. Specifically, the first plate 110 is parallel to a vertical plane, and the second plate 120 is parallel to a horizontal plane. The second plate 120 is provided with a plurality of second through holes (not shown in the figure), which are arranged at intervals along the second direction.

[0043] Furthermore, the waste liquid needle assembly 1 also includes a linear guide assembly 300. The linear guide assembly 300 is used to be fixed at any suitable position and extends in the vertical direction. The first plate 110 of the mounting base 100 is connected to the linear guide assembly 300. The mounting base 100 moves in the vertical direction on the linear guide assembly 300.

[0044] The stopper 210 is connected to the first plate 110 and is located above the second plate 120. The first through holes 211 correspond to the second through holes 211. The stopper 210 is preferably further provided with a plurality of annular grooves (not shown), each of which surrounds the outer circumference of one of the first through holes 211.

[0045] The mounting block 220 includes a base 221 and engagement posts 222. The base 221 is positioned above the second plate 120 and below the stop block 210. The engagement posts 222 are connected to the upper end of the base 221 and partially extend through the corresponding first through-holes 211. It should be understood that the second plate 120 is configured to contact the lower end of the base 221 to limit the maximum distance between the base 221 and the stop block 210, thereby preventing the mounting block 220 from disengaging from the corresponding first through-holes 211.

[0046] In addition, the mounting block 220 is provided with a coupling channel (not shown in the figure) that passes through the base 221 and the coupling column 222 in the vertical direction. The coupling channel is connected to a corresponding second through hole, and the coupling channel is used for the waste liquid needle 20 to pass through. In other words, the waste liquid needle 20 is partially passed through the coupling channel, and then the waste liquid needle 20 and the mounting block 220 can be locked by using a set screw or any other suitable method. It should be understood that in the waste liquid needle assembly 1, after the upper end of the waste liquid needle 20 passes through the coupling channel, it continues to extend to the top of the stop block 210. At the same time, the lower end of the waste liquid needle 20 is located outside the coupling channel and also passes through the corresponding second through hole.

[0047] Each of the elastic members 230 may include a spring, which is sleeved on a portion of the outer circumference of the engagement column 222, and the upper end of the spring contacts the lower surface of the stop block 210, and the lower end of the spring contacts the upper end of the corresponding base 221. Thus, the elastic member 230 can provide a relatively uniform force in the circumferential direction of the engagement column 222, reducing the possibility of the mounting block 220 being skewed when moving in the vertical direction. Preferably, the outer diameter of the spring is adapted to the diameter of the annular groove surrounding the outer circumference of the corresponding first through hole 211, and the upper end of the spring is accommodated in the corresponding annular groove. This arrangement can prevent the spring from moving in the radial direction of the first through hole 211.

[0048] In an alternative implementation, each of the elastic members may include a plurality of springs, and the plurality of springs are arranged at intervals around the circumference of the engagement post 222 (not shown in the drawings).

[0049] In some embodiments, the sum of the natural length of the elastic member 230 and the length of the base 221 is equal to the distance between the bottom of the annular groove and the upper surface of the second plate 120. In this way, when the waste liquid needle mounting mechanism 10 is assembled, the elastic member 230 is at its natural length. The length mentioned here refers to the size of the component in the first direction, that is, the length of the elastic member 230 refers to the size of the elastic member 230 in the first direction, and the length of the base 221 refers to the size of the base 221 in the first direction. The natural length of the elastic member 230 refers to the length of the elastic member 230 when it is not subjected to a force along the first direction.

[0050] In other embodiments, the sum of the natural length of the elastic member 230 and the length of the base 221 is greater than the distance between the bottom of the annular groove and the upper surface of the second plate 120. In this way, when the waste liquid needle installation mechanism 10 is assembled, the elastic member 230 is compressed and stores elastic potential energy.

[0051] Furthermore, the waste liquid needle mounting mechanism 10 further includes a plurality of guide stoppers (not labeled in the figure). Each of the guide stoppers is used to guide the vertical movement of the mounting block 220 and to prevent the mounting block 220 from causing the waste liquid needle 20 thereon to rotate about the axis of the corresponding first through hole 211.

[0052] Each guide stopper includes a first stopper portion 411 and a second stopper portion (not shown). The first stopper portion 411 may include a protrusion provided on the end surface of the mounting block 220 facing away from the stopper 210, the protrusion extending in the vertical direction. The second stopper portion may include a third through-hole provided in the second plate 120, the third through-hole extending vertically therethrough. The protrusion is partially disposed within the third through-hole.

[0053] In the case where the guide limiter includes the protrusion and the third through hole, it is preferred that the elastic member 230 stores elastic potential energy when the waste liquid needle mounting mechanism 10 is assembled. This is because, under some extreme working conditions, when the waste liquid needle assembly 1 does not perform the operation of removing the liquid, it is easy for at least part of the protrusion of the guide limiter to contact the hole wall of the third through hole, resulting in friction between the two, and the friction between the protrusion and the hole wall of the third through hole may cause the protrusion to move upward to disengage from the third through hole. Once this happens, the consequences include: the guide limiter loses its guide limit function, and it also causes a large deviation in the position of the multiple waste liquid needles 20 connected to the multiple mounting blocks 220. Then, when it is necessary to remove the liquid on the multiple immunoblotting detection membrane strips 30, it may be difficult for some of the waste liquid needles 20 to contact the membrane strip body of the corresponding immunoblotting detection membrane strip 30. However, when the elastic member 230 stores elastic potential energy in advance and the stored elastic potential energy is sufficient to overcome the friction force, the above situation can be avoided.

[0054] Figure 3 and Figure 4 The structure of the waste liquid needle 20 is shown. A liquid suction port 21 is formed at one end of the waste liquid needle 20, and the liquid suction port 21 is used to extend into the concave cavity 31 of the immunoblotting detection membrane strip 30 and contact the membrane strip body to absorb the liquid on the immunoblotting detection membrane strip 30. Furthermore, an air hole 22 is also formed on the side wall of the waste liquid needle 20, and the air hole 22 is adjacent to the liquid suction port 21. By providing the air hole 22, air can be introduced into the needle cavity of the waste liquid needle 20, avoiding the formation of a negative pressure chamber in the needle cavity after the liquid suction is completed, causing the membrane strip body to be adsorbed by the waste liquid needle 20 at the liquid suction port 21, thereby avoiding the situation where the immunoblotting detection membrane strip 30 is synchronously moved up when the waste liquid needle 20 is controlled to move up after the liquid removal is completed.

[0055] Alternatively, refer to Figure 3 and Figure 4 , the air hole 22 is connected to the liquid suction port 21. Of course, the air hole 22 and the liquid suction port 21 may also be disconnected.

[0056] In addition, those skilled in the art know that in actual application, the loading plate is arranged on a shaking table (not shown in the figure). Under the action of the shaking table, the third direction forms an angle with the horizontal plane, so that one end of the two opposite ends of the immunoblot detection membrane strip 30 in the third direction is higher and the other end is lower. For ease of description, the higher end is referred to as the first end and the lower end is referred to as the second end herein. When removing liquid, the waste liquid needle 20 is arranged near the second end of the concave cavity 31, and the air hole 22 is towards the second end. Such a setting mode is conducive to reducing the residual amount of liquid in the immunoblot detection membrane strip 30 and improving the liquid removal rate of the waste liquid needle assembly 1. In practice, the waste liquid needle assembly 1 is used to remove the liquid in the immunoblot detection membrane strip 30, so that the residual amount of liquid in the immunoblot detection membrane strip 30 is not more than 5%, that is, the liquid removal rate of the waste liquid needle assembly 1 can be greater than or equal to 95%.

[0057] The liquid removal rate is calculated as follows: before adding liquid to the immunoblot detection membrane strip 30, the immunoblot detection membrane strip 30 is weighed to obtain a first weight of the immunoblot detection membrane strip 30 of m1. The total mass of the liquid added to the immunoblot detection membrane strip 30 is M. Afterwards, after the liquid aspiration is completed, the immunoblot detection membrane strip 30 is weighed again to obtain a second weight m2 of the immunoblot detection membrane strip 30. Subsequently, the first weight m1 is subtracted from the second weight m2 to obtain a difference Δm between the two, where Δm is the weight of the liquid remaining on the immunoblot detection membrane strip 30. Finally, the weight Δm of the liquid remaining on the immunoblot detection membrane strip 30 is divided by the total weight M of the liquid added to the immunoblot detection membrane strip 30 to obtain the residual amount of liquid on the immunoblot detection membrane strip 30.

[0058] In addition, it can be understood that since the guide limiter of the waste liquid needle mounting mechanism 10 can prevent the mounting block 220 from driving the waste liquid needle 20 to rotate around the axis of the corresponding first through hole 211, it can be ensured that the air hole 22 of the waste liquid needle 20 is always facing the second end of the immunoblotting detection membrane strip 30.

[0059] Furthermore, an embodiment of the present invention also provides a kind of immunoblotting detection device, and the immunoblotting detection device includes a shaking table (not shown), a driving mechanism (not shown) and the aforementioned waste liquid needle assembly 1. The shaking table is used to carry a loading plate (not shown). The carrying platform is used to carry a plurality of the immunoblotting detection membrane strips 30, and a plurality of the immunoblotting detection membrane strips 30 are arranged along the second direction. The waste liquid needle assembly 1 and the loading plate are arranged along the first direction, and the liquid suction port 21 of the waste liquid needle 20 is toward the loading plate. The driving mechanism is connected to the mounting base 100, and is used to drive the waste liquid needle assembly 1 to move along the first direction, so that the liquid suction port 21 of the waste liquid needle 20 is close to or away from the immunoblotting detection membrane strip 30.

[0060] While the present invention is disclosed above, it is not limited thereto. Those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, the present invention is intended to encompass such modifications and variations as long as they fall within the scope of the claims and their equivalents.

Claims

1. A waste liquid needle installation mechanism, characterized in that: It includes a mounting substrate and a positioning assembly; wherein the positioning assembly includes: a stopper connected to the mounting substrate, and provided with a plurality of first through holes extending along a first direction, wherein the plurality of first through holes are arranged at intervals along a second direction, wherein the second direction is perpendicular to the first direction; a plurality of mounting blocks arranged in one-to-one correspondence with the plurality of first through holes; each of the mounting blocks is partially disposed in the corresponding first through hole and is movable relative to the stop block along the first direction; and a plurality of elastic members, each of the elastic members being disposed between one of the mounting blocks and the stop block, and the elastic members being adapted to expand and contract as the corresponding mounting block moves; The mounting base plate includes a first plate body and a second plate body connected to each other, the first plate body and the second plate body being perpendicular to each other; the first plate body is connected to the stopper, the second plate body is located on a side of the mounting block away from the stopper, and the second plate body is used to contact the surface of the mounting block away from the stopper and prevent the mounting block from being separated from the corresponding first through hole; The waste liquid needle mounting mechanism also includes a plurality of guide limit members, each of the guide limit members includes a first limit portion and a second limit portion, the first limit portion includes a protrusion arranged on the end face of the mounting block away from the stop block, and the protrusion extends along the first direction; the second limit portion includes a third through hole arranged on the second plate body and extending through the first direction, and the third through hole is used for the protrusion to pass through.

2. The waste liquid needle installation mechanism according to claim 1, characterized in that: The mounting block includes a base and a coupling column, the coupling column is connected to one end of the base facing the stop block, and the coupling column is partially inserted into the corresponding first through hole; the elastic member is sleeved on the coupling column, one end of the elastic member contacts the stop block, and the other end of the elastic member contacts the base.

3. The waste liquid needle installation mechanism according to claim 1 or 2, characterized in that: A plurality of annular grooves are further provided on the surface of the stopper facing the elastic member, and each of the annular grooves is arranged around one of the first through holes; and one end of the elastic member in contact with the stopper is accommodated in the corresponding annular groove.

4. The waste liquid needle installation mechanism according to claim 2, characterized in that: The sum of the natural length of the elastic member and the length of the base is greater than the distance from the point where the stopper contacts the elastic member to the surface of the second plate facing the stopper; the length of the elastic member refers to the size of the elastic member in the first direction, and the length of the base refers to the size of the base in the first direction.

5. The waste liquid needle installation mechanism according to claim 1, characterized in that: The mounting block is provided with a joining channel extending through along the first direction, and the joining channel is used for the waste liquid needle to pass through; the second plate body is provided with a second through hole extending through along the first direction, the second through hole is connected to the joining channel, and is used for the waste liquid needle to pass through.

6. A waste liquid needle assembly, characterized in that: It comprises a plurality of waste liquid needles and a waste liquid needle mounting mechanism as described in any one of claims 1 to 5, all of the waste liquid needles extend along the first direction, and each of the waste liquid needles is connected to one of the mounting blocks.

7. The waste liquid needle assembly according to claim 6, characterized in that: A liquid suction port is formed at the end of the waste liquid needle away from the stopper, and an air hole is further provided on the side wall of the waste liquid needle. The air hole is adjacent to the liquid suction port and is communicated with the liquid suction port.

8. An immunoblotting detection device, characterized in that include: A shaking table, a driving mechanism and a waste liquid needle assembly as described in claim 6 or 7; the shaking table is used to carry a carrier plate, the carrier plate is used to carry multiple immunoblot detection membrane strips, and the multiple immunoblot detection membrane strips are arranged along the second direction; the waste liquid needle assembly and the carrier plate are arranged along the first direction so that the liquid aspiration port of the waste liquid needle faces the carrier plate; the driving mechanism is connected to the mounting base plate of the waste liquid needle assembly, and is used to drive the waste liquid needle assembly to move along the first direction so that the liquid aspiration port of the waste liquid needle is close to or away from the immunoblot detection membrane strip.

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