Active molecular motor, cream composition with anti-wrinkle and anti-aging effects, preparation method and application thereof

Abstract

The present invention discloses an active molecular motor, a cream composition, and its preparation and application with anti-wrinkle and anti-aging effects. The active molecular motor with anti-wrinkle and anti-aging effects comprises hexapeptide-11, arginine / lysine polypeptide, elastin, and molecular motor in a mass ratio of (0.1-2.0):(0.1-0.4):(2.2-7.3):(0.3-4.7). The raw material components contained in the cream composition are as follows: 0.1-15.7% of the active molecular motor with anti-wrinkle and anti-aging effects, 1-20% of a humectant, 1-12% of an emulsifier, 1-13.6% of an emollient, 1-10% of a thickener, 0.1-1% of a preservative, 0.01-1% of a pH regulator, and the balance being deionized water. Compared with the prior art, the components of the present invention cooperate synergistically, can solve the problems of poor permeability and long effective period of existing anti-aging products, achieve an immediate anti-wrinkle effect, and can promote the synthesis and supplementation of skin collagen, elastin, and extracellular matrix components after long-term use, restore the elastic structure of the skin, and reduce skin fine lines and wrinkles.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an active molecular motor, a cream composition, a preparation method and an application thereof with anti-wrinkle and anti-aging effects. Background Technique

[0002] Staying young forever has been the wish of mankind since ancient times. Normal young skin is smooth and elastic. However, as people age, the skin, especially the facial skin, begins to show signs of aging continuously. Collagen and elastin that support the skin structure decrease and break continuously, making the dermis of the skin thinner, reducing skin elasticity and gradually causing wrinkles. The elasticity of the skin depends on the elastic fibers in the inner layer of the skin, which are equivalent to countless rubber bands, supporting the elastic fiber network of the skin tissue. Among them, about 90% of the elastic fibers are composed of elastin. Therefore, the amount of elastin determines the elasticity of the skin and the appearance of wrinkles. It can be seen that the reduction of elastin is an important reason for skin aging.

[0003] At present, the existing anti-aging products on the market have defects such as poor permeability and long effective periods. Therefore, there is still a need for a cream product with good transdermal absorption effect, capable of instant wrinkle removal and long-term anti-aging. Summary of the Invention

[0004] The purpose of the present invention is to overcome the above-mentioned defects existing in the prior art and provide an active molecular motor, a cream composition, a preparation method and an application thereof with anti-wrinkle and anti-aging effects, and the cream composition can instantaneously remove wrinkles and has long-term anti-aging effects.

[0005] The purpose of the present invention can be achieved by the following technical solutions: An active molecular motor with anti-wrinkle and anti-aging effects, comprising hexapeptide-11, arginine / lysine polypeptide, elastin and a molecular motor in a mass ratio of (0.1-2.0):(0.1-0.4):(2.2-7.3):(0.3-4.7).

[0006] Preferably, the mass ratio of the hexapeptide-11, arginine / lysine polypeptide, elastin and the molecular motor is 0.2:0.2:6.4:1.2.

[0007] Preferably, the molecular motor is a fermentation product of Thermus thermophilus.

[0008] Hexapeptide-11 is a synthetic peptide widely used as an anti-aging ingredient in cosmetics, which can act on dermal fibroblasts, promote the synthesis of collagen, elastin and extracellular matrix components, thereby increasing skin elasticity and making the skin feel firm.

[0009] Arginine / lysine polypeptide, also known as conotoxin, can inhibit the release of acetylcholine, temporarily cut off the signal between nerves and muscles, relax the muscles, and instantly reduce the generation of expression lines. The efficacy of conotoxin is not inferior to that of botulinum toxin, but it can provide a natural wrinkle-removing effect, and is safer and more controllable in effect.

[0010] Elastin, also called elastic protein, is an important component of the dermal tissue of the skin. Adding elastin to skin care products can supplement the content of elastin in the skin and enhance the elasticity and softness of the skin. The elastin selected in the present invention is elastin in the cosmetic raw material catalog, and common elastin on the market can be selected. Recombinant elastin that is skin-homologous, highly active, highly pure, highly stable, water-soluble, and transdermal is preferably selected.

[0011] Molecular motor is a transmembrane protein widely present in cells, with the phospholipid bilayer structure of the cell membrane, and can transport the components required for cell metabolism. As a substance transport carrier, it can transport adenosine triphosphate (ATP) synthase, proteins, DNA, and other bioactive ingredients that are difficult to directly enter cells. Molecular motors can generate ATP, provide the energy required for the skin to absorb active substances, and the phospholipid bilayer structure can also encapsulate water-soluble and lipid-soluble active ingredients, so the transdermal absorption rate of active ingredients can be greatly increased.

[0012] The present invention also provides a preparation method of the active molecular motor with wrinkle-removing and anti-aging effects. Mix hexapeptide-11, arginine / lysine polypeptide, elastin, and molecular motor evenly according to the mass ratio, and the active molecular motor with wrinkle-removing and anti-aging effects can be obtained.

[0013] The present invention also provides a cream composition containing the active molecular motor with wrinkle-removing and anti-aging effects. The cream composition includes the following raw material components in weight percentages: 0.1-15.7% of the active molecular motor with wrinkle-removing and anti-aging effects, 1-20% of a moisturizer, 1-12% of an emulsifier, 1-13.6% of an emollient, 1-23.2% of a thickener, 0.1-1% of a preservative, 0.01-1% of a pH regulator, and the balance is deionized water.

[0014] Preferably: the moisturizer is selected from one or more of hyaluronic acid, glycerin, butanediol, sorbitol, honey, inositol, urea, panthenol, trehalose, ceramide, erythritol;

[0015] The emollient is selected from one or more of squalene, beeswax, jojoba seed oil, castor oil, shea butter;

[0016] The emulsifier is selected from one or more of beeswax, lecithin, lanolin, wool alcohol, glyceryl stearate, PEG-100 stearate, cetearyl glucoside;

[0017] The thickener is selected from one or more of carbomer, xanthan gum, modified starch, high molecular polymer;

[0018] The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, pentylene glycol;

[0019] The pH regulator is one or more of lactic acid, arginine, sodium hydroxide, potassium hydroxide, triethanolamine.

[0020] The present invention also provides a preparation method of a cream composition, comprising the following steps:

[0021] 1) Dissolve the thickener with deionized water, stir and disperse evenly, add the humectant to the obtained mixed solution, stir and heat to 70 - 85 °C, and stir and disperse evenly to obtain an aqueous phase material;

[0022] 2) Dissolve the emollient and the emulsifier with deionized water, stir and disperse evenly, then heat to 70 - 85 °C until completely dissolved to obtain an oil phase material;

[0023] 3) Slowly add the oil phase material to the aqueous phase material, continue to homogenize for 5 - 20 min, and then sequentially add the pH value regulator and the preservative;

[0024] 4) Stir and cool the mixed solution in step 3) to 40 - 50 °C, add the active molecular motor with anti-wrinkle and anti-aging effects, and homogenize for 5 - 10 min; after the homogenization is completed, stir and cool to 30 - 40 °C at a speed of 200 - 300 r / min to obtain a cream composition containing the active molecular motor with anti-wrinkle and anti-aging effects.

[0025] Preferably, the cream composition has the effects of instant anti-wrinkle and long-term anti-aging.

[0026] The present invention also provides a cosmetic, and the cosmetic contains the active molecular motor with anti-wrinkle and anti-aging effects.

[0027] Preferably, the cosmetic includes essence and facial mask.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) The present invention contains various active ingredients with anti-aging and anti-wrinkle effects such as hexapeptide-11, arginine / lysine polypeptide, and elastin, and cooperates with a molecular motor as a carrier to quickly and durably deliver the active ingredients to the skin, so as to achieve instant anti-wrinkle and long-term anti-aging of the skin.

[0030] (2) Based on the mechanism of skin aging and wrinkle formation, on the one hand, the arginine / lysine polypeptide can inhibit the release of acetylcholine, temporarily cut off the signal between nerves and muscles, relax the muscles, and immediately reduce the formation of expression lines (long-term use also has effects); on the other hand, hexapeptide-11 acts on dermal fibroblasts to promote the expression of protein regulatory genes, thereby promoting the synthesis of collagen and elastin, increasing skin elasticity; at the same time, adding elastin can further supplement the content of elastin in the skin, increase skin softness, and restore the elastic structure of the skin.

[0031] (3) The anti-aging efficacy ingredient composition of the present invention preferably includes hexapeptide-11, arginine / lysine polypeptide, and elastin. Through the preferred combination and efficacy testing of the cosmetics, the most preferred composition ratio is selected to maximize the anti-aging effect.

[0032] (4) The present invention uses a molecular motor with good physiological compatibility and high safety as the carrier material. Its phospholipid bilayer structure can not only encapsulate active ingredients such as hexapeptide-11, arginine / lysine polypeptide, and elastin, but also generate ATP to provide the energy required for the skin to absorb the efficacy ingredients, greatly increasing the transdermal absorption of the active anti-wrinkle and anti-aging ingredients, solving the problems of poor permeability and long effective period of existing anti-aging products, achieving an immediate anti-wrinkle effect. Long-term use can promote the synthesis and supplementation of skin collagen, elastin, and extracellular matrix components, restore the elastic structure of the skin, and reduce skin fine lines and wrinkles. Detailed implementation mode

[0033] The following examples further illustrate various implementation schemes within the scope of the present invention. These examples are given for illustrative purposes only and are not used to limit the present invention, because many changes can be made to the present invention without departing from its spirit and scope.

[0034] Unless otherwise specified, all raw materials used in the present invention are commercially available products or prepared through existing technologies:

[0035] In the following specific implementations:

[0036] Hexapeptide-11 is a product of Gil Biochemical (Shanghai) Co., Ltd.;

[0037] Arginine / lysine polypeptide is a product of Nanjing Xianda Pharmaceutical Technology Co., Ltd.;

[0038] Elastin is a product of Shanghai Kaida Biotechnology Co., Ltd.;

[0039] The molecular motor is a product of Hangzhou Youmada Biotechnology Co., Ltd.

[0040] Examples 1 - 6

[0041] The present invention relates to a cream composition capable of instant wrinkle removal and long-term anti-aging. The formulations of Examples 1 to 6 are shown in Table 1.

[0042] Unless otherwise specified, the following percentages (%) are all mass percentages.

[0043] Table 1 Formulation table of cream compositions of Examples 1 to 6 containing active molecular motors with wrinkle removal and anti-aging effects (unit: mass%)

[0044]

[0045]

[0046] For the above cream compositions of each example, the preparation steps are as follows:

[0047] 1) Weigh hexapeptide-11, arginine / lysine polypeptide, elastin and molecular motor in a certain mass ratio and mix them evenly at 4000 r / min for 15 min to obtain an active molecular motor with wrinkle removal and anti-aging effects;

[0048] 2) Dissolve the thickener with deionized water, stir and disperse it evenly, then add the moisturizer to the above mixture, stir and heat to 80 °C, and stir and disperse it evenly to obtain an aqueous phase material;

[0049] 3) Dissolve the emollient and emulsifier with deionized water, stir and disperse them evenly and then heat to 80 °C until completely dissolved to obtain an oil phase material;

[0050] 4) Slowly add the oil phase material to the aqueous phase material, continue homogenization for 10 min, and then add the pH regulator and preservative in sequence;

[0051] 5) Stir and cool the mixed material obtained in step 4) to 45 °C, add the active molecular motor with wrinkle removal and anti-aging effects, and homogenize for 8 min; after homogenization is completed, stir and cool to 30 - 40 °C at a speed of 2100 r / min to obtain a cream composition containing an active molecular motor with wrinkle removal and anti-aging effects.

[0052] Comparative Examples 1 to 6

[0053] Comparative Examples 1 to 6 are anti-aging creams. Except that the composition of the active molecular motor with wrinkle removal and anti-aging effects and the content of deionized water are different from those of Example 4, the rest of the formulations are the same as those of Example 4, and their preparation methods are also the same as those of Example 4. The formulation of the active molecular motor with wrinkle removal and anti-aging effects is shown in Table 2.

[0054] Table 2 Raw material component ratios of Comparative Examples 1 to 6 (unit: mass%)

[0055]

[0056] Effect verification experimental example

[0057] (1) Stability evaluation

[0058] Test samples: Examples 1 - 6

[0059] Test method: For heat resistance test, place the cream at 45 ± 2°C for three months and observe the appearance, color, and smell of the cream; for cold resistance test, place the cream at -18 ± 2°C for three months and observe the appearance, color, and smell of the cream; for heat and cold cycling test, store the cream at 45°C, room temperature, and -18°C for 24 hours each and cycle 3 times, then observe the appearance, color, and smell of the cream. The test results are shown in Table 3.

[0060] Table 3 Stability test results

[0061]

[0062] As can be seen from Table 3, the cream composition of the present invention has excellent stability and can ensure unchanged properties under high temperature, low temperature, and high - low temperature cycling conditions.

[0063] (2) Transdermal effect characterization

[0064] The permeation ability of hexa - peptide - 11, arginine / lysine polypeptide, elastin, and molecular motor in the anti - aging creams of Example 4 and Comparative Examples 4 and 6 was characterized using a transdermal diffusion cell. The specific operation is as follows: Place 7 mL of PBS buffer solution in the sample receiving pool of the transdermal diffusion cell, keep the temperature of the receiving pool constant at 32°C, stir the solution in the receiving pool at a speed of 500 rpm, and fix the 3D epidermal skin model on the surface of the receiving pool of the transdermal diffusion cell. Subsequently, apply the anti - aging creams of Example 4 and Comparative Examples 4 and 6 on the skin surface, and then use high - performance liquid chromatography (HPLC) to test the concentration of the sample in the diffusion receiving pool after 1 hour to determine the cumulative transdermal amount of hexa - peptide - 11, arginine / lysine polypeptide, and elastin within 1 hour. The test results are as follows:

[0065] Table 4 Comparison of transdermal rates of active ingredients in the anti - aging creams of Example 4 and Comparative Examples 4 and 6

[0066]

[0067] The results show that the anti-aging active ingredients hexapeptide-11, arginine / lysine polypeptide, and elastin in the anti-aging cream of Example 4 have good transmittance in the skin, and the transdermal rate can reach over 80% in about 1 hour. This is because the molecular motor design is added, and the molecular motor can generate ATP and provide the energy required for the skin to absorb anti-aging active substances. Therefore, the transdermal absorption rate of the anti-aging active ingredients hexapeptide-11, arginine / lysine polypeptide, and elastin can be significantly increased. In Comparative Example 4 and Comparative Example 6, the molecular motor was not added to the anti-aging cream, and anti-aging active ingredients such as hexapeptide-11, arginine / lysine polypeptide, and elastin are difficult to directly penetrate the skin stratum corneum, so the transdermal absorption efficiency is low (<20%).

[0068] (3) Evaluation of anti-aging and wrinkle-reducing effects

[0069] Test method: 360 healthy women (45 - 55 years old) were selected and divided into 12 groups, with 30 people in each group. The creams in Examples 1 - 6 and Comparative Examples 1 - 6 were used respectively. The participants used the samples twice a day, morning and evening, for 56 days. During the research period, the participants were not allowed to use other products, and the test results were evaluated after 56 days of using the samples. Before the start of the experiment, the skin wrinkles and skin elasticity of the participants were measured; after 7 days, 14 days, 28 days, and 56 days of using the cream products, the skin wrinkles and skin elasticity of the participants were measured again. Among them, the facial skin wrinkles were measured using a skin image analysis system (Skin Visiometer SV600); the skin elasticity was measured using a dual MPA580 skin elasticity detector, and the average value of 30 people in each group was calculated. The dual MPA580 was used for measurement, and the average value of 30 people in each group was calculated.

[0070] Wrinkle change rate (%) = (average wrinkle value of each group after using the cream in Example / Comparative Example - average wrinkle value of each group before use) / average wrinkle value of each group before use * 100%. In Table 5, the wrinkle improvement rate is the positive value obtained after calculating the change rate by the formula

[0071] Skin elasticity improvement rate (%) = (average skin elasticity value of each group after using the cream in Example / Comparative Example - average skin elasticity value of each group before use) / average skin elasticity value of each group before use * 100%

[0072] The results are shown in Table 5 and Table 6. Immediately after use in Table 5 refers to the detection immediately after the first use.

[0073] Table 5 Wrinkle improvement rate of creams in Examples 1 - 6 and Comparative Examples 1 - 6 after using different times

[0074]

[0075] Table 6 Skin elasticity improvement rate of creams in Examples 1 - 6 and Comparative Examples 1 - 6 after using different times

[0076]

[0077] As can be seen from Table 5 and Table 6, the creams in Examples 1 to 6 can achieve excellent instant anti-wrinkle effects after being used once. The longer the using time, the better the anti-wrinkle effect. At the same time, the creams in Examples 1 to 6 can also achieve good effects in improving skin elasticity. In addition, the anti-wrinkle and anti-aging performances of Examples 1 to 6 are significantly better than those of Comparative Examples 1 to 6, indicating that the anti-aging composition of the present invention requires three active ingredients (Hexapeptide-11, Arginine / Lysine Polypeptide, Elastin) and a molecular motor to cooperate in order to best exert the anti-wrinkle and anti-aging effects of the active ingredients. In addition, when the contents of the three active ingredients are optimized to a special ratio, the anti-wrinkle and anti-aging effects of the active efficacy substances can be maximally exerted. In summary, the composition of the present invention has the effects of instant anti-wrinkle and long-term anti-aging.

[0078] The above description of the embodiments is for the convenience of those of ordinary skill in the art to understand and use the invention. Obviously, those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative labor. Therefore, the present invention is not limited to the above embodiments, and the improvements and modifications made by those skilled in the art without departing from the scope of the present invention according to the disclosure of the present invention should be within the protection scope of the present invention.

Claims

1. An active molecular motor with anti-wrinkle and anti-aging effects, characterized in that, It includes hexapeptide-11, arginine / lysine polypeptide, elastin and molecular motor with a mass ratio of (0.1-2.0):(0.1-0.4):(2.2-7.3):(0.3-4.7); the molecular motor is selected from the fermentation product of Thermus thermophilus of Hangzhou Youmada Biotechnology Co., Ltd.

2. The active molecular motor with anti-wrinkle and anti-aging effects according to claim 1, characterized in that, The mass ratio of the hexapeptide-11, arginine / lysine polypeptide, elastin and molecular motor is 0.2:0.2:6.4:1.

2.

3. A preparation method of an active molecular motor with anti-wrinkle and anti-aging effects as described in claim 1, characterized in that, Mix the hexapeptide-11, arginine / lysine polypeptide, elastin and molecular motor evenly according to the mass ratio, and an active molecular motor with anti-wrinkle and anti-aging effects can be obtained.

4. A cream composition containing the active molecular motor with anti-wrinkle and anti-aging effects as described in claim 1, characterized in that, This cream composition includes the following raw material components in weight percentages: 0.1~15.7% of the active molecular motor with anti-wrinkle and anti-aging effects, 1~20% of a moisturizer, 1~12% of an emulsifier, 1~13.6% of an emollient, 1~10% of a thickener, 0.1~1% of a preservative, 0.01~1% of a pH regulator, and the balance is deionized water.

5. The cream composition according to claim 4, wherein: The moisturizer is selected from one or more of the following: hyaluronic acid, glycerol, butanediol, sorbitol, honey, inositol, urea, panthenol, trehalose, ceramide, erythritol; The emollient is selected from one or more of the following: squalene, beeswax, jojoba seed oil, castor oil, shea butter; The emulsifier is selected from one or more of the following: beeswax, lecithin, lanolin, lanolin alcohol, glyceryl stearate, PEG-100 stearate, cetearyl glucoside; The thickener is selected from one or more of the following: carbomer, xanthan gum, modified starch, polymer; The preservative is selected from one or more of the following: phenoxyethanol, ethylhexylglycerin, pentylene glycol; The pH regulator is one or more of lactic acid, arginine, sodium hydroxide, potassium hydroxide, triethanolamine.

6. A method for preparing a cream composition as claimed in claim 4, characterized in that, It includes the following steps: 1) Dissolve the thickener in deionized water, stir and disperse evenly, add the moisturizer to the obtained mixed solution, stir and heat to 70-85 °C, and stir and disperse evenly to obtain an aqueous phase material; 2) Dissolve the emollient and emulsifier in deionized water, stir and disperse evenly and then heat to 70-85 °C until completely dissolved to obtain an oil phase material; 3) Slowly add the oil phase material to the aqueous phase material, continue to homogenize for 5-20 min, and then add the pH value regulator and preservative in sequence; 4) Stir and cool the mixed solution in step 3) to 40-50 °C, add the active molecular motor with anti-wrinkle and anti-aging effects, and homogenize for 5-10 min; after homogenization, stir and cool to 30-40 °C at a speed of 200-300 r / min, and the cream composition containing the active molecular motor with anti-wrinkle and anti-aging effects is obtained.

7. The preparation method of the cream composition according to claim 6, characterized in that, The cream composition has the effects of instant anti-wrinkle and long-term anti-aging.

8. A cosmetic, characterized in that, The cosmetic contains the active molecular motor with anti-wrinkle and anti-aging effects as described in claim 1.

9. The cosmetic according to claim 8, characterized in that, The cosmetic includes essence and mask.

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