Anti-aging composition containing natural nmn and use thereof

Abstract

The application provides an anti-aging composition containing natural NMN and application thereof, and relates to the technical field of food health care.The anti-aging composition containing natural NMN provided by the application is mainly composed of pine pollen extract, sunflower pollen extract, Chinese wolfberry extract and rose extract in a specific ratio, fully plays a synergistic effect among the components, has good anti-aging ability, can effectively resist oxidative damage, prolongs the lifespan of nematodes under oxidative stress, and reduces the deposition of peroxidation product lipofuscin in nematodes.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food health care technology, and in particular to an anti-aging composition containing natural NMN and application thereof. BACKGROUND

[0002] Aging refers to the process of normal physiological functions of a biological individual declining with age, and the decline is reflected in the degenerative changes of functions of various tissues and organs of the body and the decline of the body's ability to maintain dynamic balance. Due to the decline of the body's functions, diseases such as hypertension, diabetes, atherosclerosis, and senile dementia occur and develop, and eventually lead to death. Aging is a natural physiological process of a living body, and in order to pursue the quality and length of life, anti-aging has become a subject pursued by mankind. The main solution to anti-aging is the exploration of aging mechanisms and the development and research of anti-aging substances. At present, substances confirmed to have anti-aging effects include ginsenosides, lycium polysaccharides, spilanthes, polygonatum polysaccharides, and ganoderma lucidum polysaccharides. These substances are widely used in health foods or functional foods.

[0003] NMN is a new anti-aging component discovered in recent years. NMN (Nicotinamide mononucleotide) is the abbreviation of nicotinamide mononucleotide, which is an inherent substance in the human body and also exists in some fruits and vegetables. In the human body, NMN is the precursor of NAD+, and its function is embodied through NAD+. NAD+ is also called coenzyme I, and its full name is nicotinamide adenine dinotide. It is a very important substance in the body and exists in every cell to participate in thousands of reactions such as the tricarboxylic acid cycle, DNA repair, long-life protein synthesis, and ribose synthetase synthesis. The NAD+ in the body rapidly decreases with age, about 50% every 20 years, and as the NAD+ in the body decreases, aging will also accelerate. Supplementing NAD+ is the main means to solve aging, and there are four ways to supplement NAD+, which are nicotinic acid, tryptophan, nicotinamide, and NMN / NR. Due to the influence of the metabolic pathway in the body, supplementing NMN is the most effective and economical way.

[0004] A large number of documents show that pollen has anti-aging effects, including pine pollen and bee pollen. The "Shen Nong Ben Cao Jie" describes pine pollen as having "warm, sweet, and non-toxic. It can moisten the heart and lungs, benefit qi, dispel wind, and stop bleeding." A large number of modern studies have shown that pine pollen has the effects of enhancing immunity, regulating metabolism, antioxidant, anti-aging, and heavy metal excretion. Bee pollen has a history of thousands of years of consumption in China and is widely used in the regulation of endocrine, antioxidant, liver protection, and anti-aging. The anti-aging mechanism of pollen has been continuously researched and explored, and the latest research shows that pine pollen and sunflower pollen contain NMN components, which is one of the reasons why pollen has anti-aging effects.

[0005] Medlar is a traditional Chinese medicinal material with clear health care effect. Medlar is rich in nutrients, including polysaccharides, flavonoids, anthocyanins and other nutrients. Medlar mainly exerts effects through medlar polysaccharides. In recent years, a large number of studies have shown that medlar polysaccharides have the pharmacological effects of lowering blood sugar, lowering blood lipids, resisting tumors, inhibiting cell apoptosis and resisting aging.

[0006] Rose is a nutrient material with the functions of food, medicine and beautification. According to Food Herbal, rose flower is good for lung and spleen, liver and gallbladder, and can dispel evil qi. Rose flower is sweet and fragrant, and can make people feel energetic. According to Compendium of Materia Medica, rose flower can promote blood circulation, regulate qi and menstruation, and nourish the body and beautify the skin. Therefore, rose flower has many health care effects, such as promoting blood circulation, removing blood stasis, removing toxins, beautifying the skin, aiding digestion and resisting aging. Rose flower contains starch, fat, protein, amino acids, vitamins, trace elements and other nutrients, as well as active ingredients such as alcohol, esters, organic acids and pigments, which ensure the high nutritional value of rose flower.

[0007] However, the above-mentioned drugs alone have unsatisfactory anti-aging effect, and it is necessary to develop a product with better anti-aging effect.

[0008] Therefore, the present application is proposed. SUMMARY

[0009] The first object of the present application is to provide an anti-aging composition containing natural NMN to solve at least one of the above problems.

[0010] The second object of the present application is to provide an application of the anti-aging composition containing natural NMN.

[0011] In the first aspect, the present application provides an anti-aging composition containing natural NMN, which comprises the following components by weight fraction: pine pollen extract 50-150 parts, sunflower pollen extract 50-150 parts, medlar extract 25-75 parts and rose flower extract 25-50 parts.

[0012] As a further technical solution, it comprises the following components by weight fraction: pine pollen extract 100 parts, sunflower pollen extract 100 parts, medlar extract 25 parts and rose flower extract 50 parts.

[0013] As a further technical solution, the pine pollen extract comprises pine pollen water extract.

[0014] Preferably, the NMN content in the pine pollen extract is 5-50 μg / g.

[0015] Preferably, the sunflower pollen extract comprises sunflower pollen water extract.

[0016] Preferably, the NMN content in the sunflower pollen extract is 10-100 μg / g;

[0017] Preferably, the wolfberry extract comprises a wolfberry water extract;

[0018] Preferably, the wolfberry extract has a crude polysaccharide content of 100-300 mg / g;

[0019] Preferably, the rose flower extract comprises a rose flower water extract;

[0020] Preferably, the NMN content in the rose flower extract is 5-30 μg / g.

[0021] As a further technical solution, the preparation method of the pine pollen water extract or the sunflower pollen water extract comprises: extracting raw materials with water, and then preparing the pine pollen water extract or the sunflower pollen water extract through solid-liquid separation and drying treatment;

[0022] The raw materials are broken pine pollen or broken sunflower pollen.

[0023] As a further technical solution, the solid-liquid ratio of the raw materials and water is 1 g:10 mL-1 g:40 mL;

[0024] Preferably, the extraction is carried out under ultrasonic conditions;

[0025] Preferably, the power of the ultrasonic is 300-500 w, the temperature of the ultrasonic is 30-60 °C, and the time of the ultrasonic is 20-60 min;

[0026] Preferably, the solid-liquid separation comprises centrifugation or filtration;

[0027] Preferably, the filtration is carried out using a filter membrane with a molecular weight of 1000-100000 Da.

[0028] As a further technical solution, the preparation method of the wolfberry water extract comprises the following steps:

[0029] a. treating wolfberry powder with ethanol, collecting the solid after solid-liquid separation;

[0030] b. extracting the solid in step a with water, collecting the clear liquid after solid-liquid separation, and then preparing the wolfberry water extract through drying treatment.

[0031] As a further technical solution, the solid-liquid ratio of the wolfberry powder and ethanol is 1 g:10 mL-1 g:30 mL;

[0032] Preferably, the solid-liquid ratio of the solid and water is 1 g:20 mL-1 g:50 mL;

[0033] Preferably, the temperature of the extraction is 80-100℃.

[0034] Preferably, the method of solid-liquid separation comprises centrifugation or filtration.

[0035] As a further technical solution, the preparation method of the rose flower water extract comprises: extracting rose petal powder with water, then collecting the supernatant through solid-liquid separation, and then preparing the rose flower water extract through drying treatment.

[0036] As a further technical solution, the solid-liquid ratio of the rose petal powder and water is 1g:10mL-1g:30mL.

[0037] Preferably, the extraction is carried out under ultrasonic conditions.

[0038] Preferably, the power of the ultrasonic is 300-500w, the temperature of the ultrasonic is 30-60℃, and the time of the ultrasonic is 20-60min.

[0039] Preferably, the method of solid-liquid separation comprises centrifugation or filtration.

[0040] In a second aspect, the present application provides the above anti-aging composition for use in any one of a-c:

[0041] a. preparing an anti-aging product;

[0042] b. preparing a product against peroxidation damage;

[0043] c. preparing a product for inhibiting the deposition of lipofuscin.

[0044] Compared with the prior art, the present application has the following beneficial effects:

[0045] The anti-aging composition containing natural NMN provided by the present application is mainly composed of pine pollen extract, sunflower pollen extract, medlar extract and rose extract in a specific ratio, fully plays the synergistic effect between the components, has good anti-aging ability, can effectively resist oxidative damage, prolongs the lifespan of nematodes under oxidative stress, and reduces the deposition of lipofuscin, a peroxidation product, in nematodes. BRIEF DESCRIPTION OF DRAWINGS

[0046] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the drawings needed in the specific embodiments or prior art description will be briefly introduced below. Obviously, the drawings in the following description are some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0047] Figure 1Effects of the anti-aging composition containing natural NMN on the lifespan of the nematodes under oxidative stress;

[0048] Figure 2 Effects of the composition for the control group on the lifespan of the nematodes under oxidative stress;

[0049] Figure 3 Effects of the anti-aging composition containing natural NMN on the lipofuscin of the aging nematodes. DETAILED DESCRIPTION

[0050] The embodiments of the present application will be described in detail below with reference to the embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only used to illustrate the present application and should not be regarded as limiting the scope of the present application. Based on the examples in the present application, all other examples obtained by those of ordinary skill in the art without making creative efforts fall within the scope of the present application. If the specific conditions are not specified, the conventional conditions or the conditions recommended by the manufacturer are used. If the manufacturers of the reagents or instruments are not specified, they are all conventional products that can be purchased on the market.

[0051] In a first aspect, the present application provides an anti-aging composition containing natural NMN, comprising the following components by weight fraction: pine pollen extract 50-150 parts, sunflower pollen extract 50-150 parts, wolfberry extract 25-75 parts, and rose extract 25-50 parts.

[0052] In the anti-aging composition of the present application, the weight fraction of pine pollen extract may, for example, be, but is not limited to, 50 parts, 70 parts, 90 parts, 110 parts, 130 parts, or 150 parts; the weight fraction of sunflower pollen extract may, for example, be, but is not limited to, 50 parts, 70 parts, 90 parts, 110 parts, 130 parts, or 150 parts; the weight fraction of wolfberry extract may, for example, be, but is not limited to, 25 parts, 35 parts, 45 parts, 55 parts, 65 parts, or 75 parts; and the weight fraction of rose extract may, for example, be, but is not limited to, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, or 50 parts.

[0053] The anti-aging composition containing natural NMN provided by the present application fully plays the synergistic effect between pine pollen extract, sunflower pollen extract, wolfberry extract, and rose extract. The inventors have found that the anti-aging composition has good anti-aging ability, can effectively resist oxidative damage, prolong the lifespan of nematodes under oxidative stress, and reduce the deposition of lipofuscin, a peroxidation product, in nematodes.

[0054] In some preferred embodiments, the following components by weight fraction are included: pine pollen extract 100 parts, sunflower pollen extract 100 parts, wolfberry extract 25 parts, and rose extract 50 parts.

[0055] By further optimizing and adjusting each component in the anti-aging composition, the anti-aging effect of the composition is better.

[0056] In some preferred embodiments, the pine pollen extract comprises a pine pollen water extract; the NMN content in the pine pollen extract may be, for example, but not limited to, 5 μg / g, 10 μg / g, 20 μg / g, 30 μg / g, 40 μg / g, or 50 μg / g;

[0057] The sunflower pollen extract comprises a sunflower pollen water extract; the NMN content in the sunflower pollen extract may be, for example, but not limited to, 10 μg / g, 20 μg / g, 40 μg / g, 60 μg / g, 80 μg / g, or 100 μg / g;

[0058] The wolfberry extract comprises a wolfberry water extract; the crude polysaccharide content in the wolfberry extract may be, for example, but not limited to, 100 mg / g, 150 mg / g, 200 mg / g, 250 mg / g, or 300 mg / g;

[0059] The rose flower extract comprises a rose flower water extract; the NMN content in the rose flower extract may be, for example, but not limited to, 5 μg / g, 10 μg / g, 15 μg / g, 20 μg / g, 25 μg / g, or 30 μg / g.

[0060] In some preferred embodiments, the preparation method of the pine pollen water extract or the sunflower pollen water extract comprises: extracting raw materials with water, then performing solid-liquid separation to collect the supernatant, and preparing the pine pollen water extract or the sunflower pollen water extract after drying treatment;

[0061] The raw material is broken pine pollen or broken sunflower pollen.

[0062] The preparation method is simple, and the pine pollen water extract or the sunflower pollen water extract prepared by the method has a high content of effective substances and good anti-aging effect.

[0063] In some preferred embodiments, the solid-liquid ratio of the raw material and water may be, for example, but not limited to, 1 g:10 mL, 1 g:20 mL, 1 g:30 mL, or 1 g:40 mL.

[0064] Preferably, the extraction is performed under ultrasonic conditions.

[0065] Preferably, the power of the ultrasound can be, but is not limited to, 300w, 400w or 500w, the temperature of the ultrasound can be, but is not limited to, 30℃, 40℃, 50℃ or 60℃, and the time of the ultrasound can be, but is not limited to, 20min, 30min, 40min, 50min or 60min.

[0066] Preferably, the solid-liquid separation includes, but is not limited to, centrifugation or filtration, or other solid-liquid separation methods known to those skilled in the art.

[0067] Preferably, the filtration is performed using a filter membrane with a molecular weight of 1000-100000Da, and the filtrate with a molecular weight of less than 1000Da-5000Da is collected.

[0068] The drying method is not specifically limited in the present application, and any method known to those skilled in the art can be used. For example, the drying method can be as follows: the supernatant is distilled under reduced pressure to obtain a paste, and the paste is freeze-dried to obtain the extract.

[0069] Further optimization and adjustment of the preparation conditions of the pine pollen water extract or sunflower pollen water extract can increase the content of effective components in the water extract and improve the anti-aging effect.

[0070] In some preferred embodiments, the preparation method of the wolfberry water extract comprises the following steps:

[0071] a. Wolfberry powder is treated with ethanol to remove most of the non-water-soluble substances, and the solid containing polysaccharides is collected after solid-liquid separation;

[0072] b. The solid in step a is extracted with water, and then the supernatant is collected after solid-liquid separation, and the wolfberry water extract is prepared after drying treatment.

[0073] The preparation method is simple, and the wolfberry water extract prepared has a high content of effective components and good anti-aging effect.

[0074] In some preferred embodiments, the solid-liquid ratio of the wolfberry powder and ethanol can be, but is not limited to, 1g:10mL, 1g:20mL or 1g:30mL.

[0075] Preferably, the solid-liquid ratio of the solid and water can be, but is not limited to, 1g:10mL, 1g:20mL, 1g:30mL, 1g:40mL or 1g:50mL.

[0076] Preferably, the temperature of the extraction can be, but is not limited to, 80℃, 85℃, 90℃, 95℃ or 100℃.

[0077] Preferably, the method of solid-liquid separation includes, but is not limited to, centrifugation or filtration.

[0078] Through further optimization and adjustment of the preparation conditions of the water extract of wolfberry, the content of effective components in the water extract is higher, and the anti-aging effect is better.

[0079] In some preferred embodiments, the preparation method of the water extract of rose petals comprises: extracting rose petal powder with water, then collecting the supernatant through solid-liquid separation, and then preparing the water extract of rose petals through drying treatment.

[0080] The preparation method is simple, the content of effective components in the prepared water extract of rose petals is high, and the anti-aging effect is good.

[0081] In some preferred embodiments, the solid-liquid ratio of the rose petal powder and water may be, but is not limited to, 1g:10mL, 1g:20mL or 1g:30mL.

[0082] Preferably, the extraction is carried out under ultrasonic conditions.

[0083] Preferably, the power of the ultrasonic may be, but is not limited to, 300w, 400w or 500w, the temperature of the ultrasonic may be, but is not limited to, 30℃, 40℃, 50℃ or 60℃, and the time of the ultrasonic may be, but is not limited to, 20min, 30min, 40min, 50min or 60min.

[0084] Preferably, the method of solid-liquid separation includes, but is not limited to, centrifugation or filtration.

[0085] Through further optimization and adjustment of the preparation conditions of the water extract of rose petals, the content of effective components in the water extract is higher, and the anti-aging effect is better.

[0086] In a second aspect, the present application provides the above-mentioned anti-aging composition for any one of a-c:

[0087] a. preparing an anti-aging product;

[0088] b. preparing an anti-peroxidation damage product;

[0089] c. preparing a product for inhibiting the deposition of lipofuscin.

[0090] The inventors have found that the anti-aging composition containing natural NMN provided by the present application has good anti-aging ability, can effectively resist oxidative damage, prolong the lifespan of nematodes under oxidative stress, and reduce the deposition of lipofuscin, a peroxidation product, in nematodes. Therefore, the anti-aging composition can be used to prepare an anti-aging product, an anti-peroxidation damage product or a product for inhibiting the deposition of lipofuscin.

[0091] In the present invention, the products include, but are not limited to, food and medicine.

[0092] The present invention is further illustrated by the following specific examples and comparative examples, but it should be understood that these examples are only for a more detailed illustration and should not be understood as limiting the present invention in any form.

[0093] In the following examples and comparative examples (the same batch of extract is used in examples and comparative examples), the preparation methods of pine pollen extract, sunflower pollen extract, wolfberry extract and rose extract are as follows:

[0094] Pine pollen extract or sunflower pollen extract:

[0095] S1. Pretreatment: After removing sand and impurities from pine pollen and sunflower pollen, low-temperature high-speed cyclone crushing technology was applied to break the cell wall, and broken pine pollen and broken sunflower pollen were obtained;

[0096] S2. Preparation of extraction solution: pine pollen or sunflower pollen was mixed with distilled water at a ratio of 1g:(10-40)mL, and an ultrasonic generator was applied at 300-500w, 30-60℃ for 20-60min, and the filtrate was collected after filtration;

[0097] S3. Concentration of extraction solution: ultrafiltration device and 1000Da-100000Da filter membrane were applied to concentrate the filtrate of S2, and the filtrate with a molecular weight less than 1000Da-5000Da was collected;

[0098] S4. Preparation of extract: the paste obtained by reducing pressure distillation of S3 filtrate was freeze-dried to obtain the extract.

[0099] Wolfberry extract:

[0100] S1. Pretreatment: wolfberry fruits were picked, washed, dried and crushed for use;

[0101] S2. First solvent extraction: wolfberry raw powder was mixed with anhydrous ethanol at a ratio of 1g:(10-30)mL, stirred at a speed of 200-600rpm for 2-5h, filtered, and the solid was collected for use;

[0102] S3. Second solvent extraction: the obtained solid was mixed with distilled water at a ratio of 1g:(20-50)mL, stirred at a speed of 200-600rpm at 80-100℃ for 2-4h, extracted, filtered, and the supernatant was reserved for use;

[0103] S4. Preparation of extract: the extraction solution was concentrated by reducing pressure distillation to obtain extract paste, and wolfberry extract was obtained after freeze-drying.

[0104] Rose extract:

[0105] S1. Pretreatment: Rose petals were picked to remove visible impurities, washed, dried, and crushed for use;

[0106] S2. Preparation of extraction liquid: Rose petal powder was mixed with distilled water at a ratio of 1 g to (10-30) mL. An ultrasonic generator was applied at 300-500 w and 30-60℃ for 20-60 min. The filtrate was collected after filtration.

[0107] S3. Preparation of extract: The filtrate obtained in S2 was concentrated by reduced pressure distillation to obtain an extract in the form of extract residue, which was freeze-dried.

[0108] Example 1

[0109] An anti-aging composition containing natural NMN components, by weight, pine pollen extract 150 parts, sunflower pollen extract 150 parts, wolfberry extract 25 parts, and rose extract 25 parts.

[0110] Example 2

[0111] An anti-aging composition containing natural NMN components, by weight, pine pollen extract 100 parts, sunflower pollen extract 100 parts, wolfberry extract 75 parts, and rose extract 50 parts.

[0112] Example 3

[0113] An anti-aging composition containing natural NMN components, by weight, pine pollen extract 50 parts, sunflower pollen extract 50 parts, wolfberry extract 50 parts, and rose extract 50 parts.

[0114] Example 4

[0115] An anti-aging composition containing natural NMN components, by weight, pine pollen extract 75 parts, sunflower pollen extract 75 parts, wolfberry extract 25 parts, and rose extract 50 parts.

[0116] Example 5

[0117] An anti-aging composition containing natural NMN components, by weight, pine pollen extract 100 parts, sunflower pollen extract 100 parts, wolfberry extract 25 parts, and rose extract 50 parts.

[0118] Comparative Example 1

[0119] An anti-aging drug containing only pine pollen extract.

[0120] Comparative Example 2

[0121] An anti-aging drug containing only sunflower pollen extract.

[0122] Comparative Example 3

[0123] An anti-aging medicine containing only wolfberry extract.

[0124] Comparative Example 4

[0125] An anti-aging medicine containing only rose extract.

[0126] Test Example 1

[0127] Test purpose: Determine the content of NMN in the composition.

[0128] Test method: LC-MS method was used for detection.

[0129] Detection conditions:

[0130] Instrument: Thermo Vanquish ultra-high performance liquid chromatograph, TSQ Quantis mass spectrometer;

[0131] Chromatographic column: Thermo scientific AQC18 (100 x 2.1 mm, 2 μm);

[0132] Mass spectrometry conditions: Electrospay ionization (ESI) positive ion detection.

[0133] SRM scanning mode, parent ion 335, daughter ion 123, 97.

[0134] Test results: See Table 1.

[0135] Table 1: NMN content in different composition examples

[0136]

[0137] From the data in the table, it can be seen that the content of natural NMN in each example is different, and the content of example 1 is the highest, mainly because the proportion of NMN-containing pine pollen extract and sunflower pollen extract in the composition is relatively high (about 86% by weight).

[0138] Test Example 2

[0139] Test purpose: Determine the content of crude polysaccharide in the composition.

[0140] Test method: Sulfuric acid phenol method was used for crude polysaccharide detection.

[0141] Test results: See Table 2.

[0142] Table 2: Crude polysaccharide content in different composition examples

[0143]

[0144] From the data in the table, it can be seen that there is a certain difference in the polysaccharide content in different embodiments, wherein the polysaccharide content of Example 3 is the highest, mainly because the proportion of wolfberry extract in the composition is relatively high (25% by weight), and the polysaccharide content of other extracts is relatively low due to different extraction methods.

[0145] Test Example 3

[0146] Test purpose: evaluate the effect of the anti-aging composition on the lifespan of peroxide nematodes and compare the effect with other compositions.

[0147] Test method: 30 N2 Caenorhabditis elegans in each group were synchronized to the L4 stage, and then transferred to a culture dish containing OP50 Escherichia coli and the anti-aging composition, and pre-cultured for 48 hours, and then transferred to a culture dish containing methyl violet crystal, OP50 Escherichia coli and the anti-aging composition for culture, and the timing was started, and the number of dead nematodes was counted, recorded and plotted at 8, 24, 48, 72, 96, 144 and 168 hours. A control group without composition intervention and a comparison group with other composition intervention were set up to compare the anti-aging ability after composition intervention.

[0148] Test results: see Figure 1 (Example nematode survival curve), Figure 2 (comparison example nematode survival curve).

[0149] From Figure 1 , it can be seen that the nematodes in the control group began to die in large numbers after 24 hours of culture, and the nematodes in the anti-aging composition intervention group began to die in large numbers after about 48 hours of culture, which was about 24 hours later than the control group; the average lifespan of the nematodes in the control group was 52.8h, and the final average lifespan of the nematodes after intervention of the compositions of Examples 1-5 was 72h, 57.6h, 80.4h, 61.2h and 82.7h respectively, all of which had a significant effect of prolonging the lifespan of the nematodes, and the highest prolongation of the lifespan was 56.63%, which indicated that the anti-aging composition had a prominent effect of resisting peroxide damage and could prolong the lifespan of the nematodes under oxidative stress.

[0150] From Figure 2 , it can be seen that the compositions of the comparison examples also had a certain effect on the lifespan of the nematodes, and the final average lifespan of the nematodes after intervention of the compositions of the comparison examples 1-5 was 60.6h, 53.4h, 57.2h and 55.8h respectively, and the highest prolongation of the lifespan was 14.78%, which indicated that the components in the composition provided by the present application cooperated well and had a good synergistic effect.

[0151] Test Example 4

[0152] Test purpose: evaluate the effect of the anti-aging composition on the lipofuscin of aging nematodes.

[0153] Test method:

[0154] N2 Caenorhabditis elegans were synchronized to L4 stage, transferred to a culture dish containing the composition, cultured for 8-10 days, then the nematodes were washed with M9 buffer solution, picked to a glass slide containing 5% agarose gel, anesthetized with 10 μL of levamisole solution with a concentration of 10 mmol·L-1, and pressed into a sheet. Observation was carried out under an inverted fluorescence microscope, and Image-Pro Plus software was used to quantitatively analyze the fluorescence intensity. The test also set up a blank control group.

[0155] Test results: see Figure 3 Examples.

[0156] From Figure 3 (wherein Figure 3 A in the control group, Figure 3 B in the results of Example 3, Figure 3 C in the results of Comparative Example 3) can be seen that, compared with the blank control group, after the intervention of the anti-aging composition, the fluorescence of lipofuscin in the nematodes was significantly reduced, and the lipofuscin inhibition rates of Examples 1-5 were 74.92%, 56.73%, 84.56%, 53.01%, and 78.17%, respectively, indicating that the anti-aging composition had a clear effect of inhibiting the production of lipofuscin. From Figure 3 It can be seen that the comparative composition also has an intervention effect on the production of lipofuscin in the nematodes, and the lipofuscin inhibition rates of Comparative Examples 1-4 were 30.63%, 15.48%, 38.17%, and 24.82, respectively.

[0157] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. An anti-aging composition containing natural NMN, characterized in that, By weight, it consists of the following components Composition: 100 parts pine pollen extract, 100 parts sunflower pollen extract, 25 parts wolfberry extract and 50 parts rose extract; The pine pollen extract includes pine pollen water extract; the sunflower pollen extract includes sunflower pollen water extract; the preparation method of the pine pollen water extract or sunflower pollen water extract includes: extracting the raw material with water, and then preparing the pine pollen water extract or sunflower pollen water extract after solid-liquid separation and drying. The wolfberry extract includes wolfberry aqueous extract; the preparation method of the wolfberry aqueous extract includes the following steps: a. Treat the wolfberry powder with ethanol, and collect the solids after solid-liquid separation; b. Extract the solids from step a with water, then separate the solids and liquids to collect the clear liquid, and then dry it to prepare the wolfberry water extract; The rose extract includes a water extract of rose petals; the preparation method of the water extract of rose petals includes: extracting rose petal powder with water, then separating the solid and liquid to collect the clear liquid, and then drying it to obtain the water extract of rose petals. The NMN content in the pine pollen extract is 5-50 μg / g; The sunflower pollen extract contains 10-100 μg / g of NMN. The crude polysaccharide content in the wolfberry extract is 100-300 mg / g; The rose extract contains 5-30 μg / g of NMN.

2. The anti-aging composition according to claim 1, characterized in that, The raw materials are broken-cell wall pine pollen or broken-cell wall sunflower pollen.

3. The anti-aging composition according to claim 1, characterized in that, In the preparation method of the pine pollen water extract or sunflower pollen water extract, the material-to-liquid ratio of the raw material and water is 1g:10mL-1g:40mL; The extraction was performed under ultrasonic conditions; The ultrasonic power is 300-500 W, the ultrasonic temperature is 30-60℃, and the ultrasonic time is 20-60 min; The solid-liquid separation includes centrifugation or filtration; Filtration is performed using a filter membrane with a diameter of 1000-100000 Da.

4. The anti-aging composition according to claim 1, characterized in that, In the preparation method of the wolfberry water extract, the ratio of wolfberry powder to ethanol is 1g:10mL-1g:30mL; The solid-to-water ratio is 1g:20mL-1g:50mL; The extraction temperature is 80-100℃; The solid-liquid separation method includes centrifugation or filtration.

5. The anti-aging composition according to claim 1, characterized in that, In the preparation method of the rose petal water extract, the ratio of rose petal powder to water is 1g:10mL-1g:30mL; The extraction was performed under ultrasonic conditions; The ultrasonic power is 300-500 W, the ultrasonic temperature is 30-60℃, and the ultrasonic time is 20-60 min; The solid-liquid separation method includes centrifugation or filtration.

6. The use of the anti-aging composition according to any one of claims 1-5 in the preparation of products resistant to oxidative damage.

7. The use of the anti-aging composition according to any one of claims 1-5 in the preparation of a product that inhibits lipofuscin deposition.

Images