Moisturizing composition and skin care product

Abstract

The present invention belongs to the technical field of skin care products, and discloses a moisturizing composition, which comprises, by weight percentage, 50-60% of wheat germ extract, 30-45% of bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and 1-10% of wheat germ oil. The present invention produces a synergistic skin moisturizing effect through wheat germ oil, wheat germ extract, and bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and coordinates moisturizing inside and outside from aspects such as water control, water transport, and skin external sealing, and can significantly improve the moisture content of the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a moisturizing composition and a skin care product. Background Art

[0002] Moisturizing is the most basic need of the skin. The moisture content of the healthy skin stratum corneum should be maintained at 10% to 20%. The moisture in the skin stratum corneum can moisturize the skin and maintain skin health. Insufficient moisture in the stratum corneum can cause dry skin. When the air humidity decreases, the skin stratum corneum cannot adjust sufficient moisturizing factors in time, and the activity of the oil glands also decreases, the oil on the face will decrease, so the skin will easily become tight, and fine lines will appear under the eyes and around the nose.

[0003] At present, the active substances involved in moisturizing in cosmetics are mainly occlusive agents and hygroscopic agents; occlusive agents are some insoluble lipid substances that form an oil film on the skin surface, which prevents the evaporation of water in the skin through a sealing effect, thereby achieving a moisturizing effect. Commonly used ones include mineral oil, natural plant oil, animal oil, silicone oil, etc.; hygroscopic agents are water-soluble substances that can combine with water. When the skin loses moisture, the hygroscopic agents enter the skin through the pores, and either carry moisture or wrap up the moisture that has not been lost in the skin so that it will no longer be lost, thereby achieving a moisturizing effect. Commonly used ones include glycerin, pyrrolidone carboxylic acid, hyaluronic acid, etc.

[0004] D1: Chinese patent application 201911214629.5 discloses a moisturizing and hydrating facial cream. The moisturizing and hydrating facial cream comprises the following ingredients by weight percentage: deionized water 78-82%, wheat germ oil 5-8%, avocado tree fat 1-2%, sodium hyaluronate 3-4%, ceramide 1-2%, trehalose 1-2%, squalane 1-2%, cactus extract 0.5-1%, aloe vera leaf juice 0.3-1%, emulsifier 2-3%, thickener 0.5-1%, preservative 0.1-0.15%.

[0005] The above patent uses wheat germ oil as the basic oil component of the facial cream, adds sodium hyaluronate, ceramide, trehalose, squalane, etc. as the main ingredients, and adds cactus extract, avocado tree fruit butter, aloe vera leaf juice and other functional ingredients with moisturizing and oil balancing effects. The moisturizing and hydrating facial cream has the characteristics of significant hydrating and water-locking effects. After external use, it can effectively replenish skin moisture, deeply nourish the skin, promote metabolism, and improve skin quality.

[0006] D2: Chinese patent application 202011150859.2 discloses a method for preparing a special-effect highly moisturizing emulsion, wherein hexadecanol, olive oil, a sealant, C10 triglyceride, methyl glucoside polypropylene glycol-20 ether distearate, wheat germ oil, and octyldodecanol are added to container A to obtain a mixed solution A; vitamins, hyaluronic acid, natural moisturizers, allantoin, homemade moisturizers, and deionized water are added to container B, heated to 85-95°C, stirred at 100-200r / min for 20-40min, and then cooled to 75-85°C to obtain a mixed solution B; then the mixed solution A is slowly added to the mixed solution B stirred at 50-100r / min, the temperature is controlled to 70-80°C, and cooled to 35-45°C to obtain a pre-emulsified solution; preservatives and flavors are added to the pre-emulsified solution, stirred at 50-100r / min, 35-45°C for 30-60min to obtain an emulsion.

[0007] The above patent can not only improve the moisturizing performance by adding natural moisturizers, but also nourish the skin, repair the stratum corneum cells of the skin, protect the cell membrane of the stratum corneum cells, make the skin more elastic and fuller, and eliminate wrinkles.

[0008] The above two patents disclose moisturizing compositions based on wheat germ oil, indicating that wheat germ oil is a strong moisturizing ingredient and a skin care composition with strong moisturizing effect can be developed around wheat germ oil.

[0009] However, wheat germ oil, as an oil-soluble active substance in wheat germ, generally forms a protective film on the skin in moisturizing skin care products, which can effectively isolate substances in the environment that may damage the skin and effectively protect the stratum corneum from excessive loss of moisture. In essence, wheat germ oil is a sealant; therefore, the amount of wheat germ oil should not be too much, and it needs to be used in combination with other moisturizing components such as hygroscopic agents; however, since the sources of various sealants and hygroscopic agents are not single, and the extraction methods are diverse, the obtained extracts may contain some other ingredients, and these unknown ingredients mixed together may produce other side effects, which may affect the moisturizing effect of the composition.

[0010] Based on this, D3: Chinese patent 201310549324.6 discloses a cosmetic composition with hydrating and moisturizing effects and its application, wherein the cosmetic composition comprises the following components: water-soluble wheat protein, wheat germ oil and wheat germ water-soluble extract; the weight ratio of the water-soluble wheat protein, wheat germ oil and wheat germ water-soluble extract is (0.1-10): (0.1-10): (0.1-10).

[0011] The cosmetic compositions with hydrating and moisturizing effects in the above-mentioned patents are all derived from wheat, and have excellent hydrating and moisturizing effects. It is a technical solution with a single source and excellent moisturizing effects, and has important technical implications for the research and development of moisturizing compositions with a single source.

[0012] The above patents all use a moisturizing solution that uses a combination of a sealant and a moisturizer, which can only achieve a moisturizing effect of reducing the contact of moisture with the outside world and thereby losing and locking the moisture inside the skin; since the water content of different parts of people's skin is different, although only using the above technical solution can effectively moisturize, the water content of different parts of the skin cannot be balanced, resulting in differences in skin quality in different parts of the skin.

[0013] Therefore, it is necessary to develop a moisturizing composition that can effectively improve the skin moisturizing effect and balance the water content of various parts of the skin. Summary of the invention

[0014] One of the purposes of the present invention is to provide a moisturizing composition to solve the problem that the prior art cannot effectively improve the moisturizing effect while balancing the water content of various parts of the skin and improving the skin quality. The moisturizing composition not only has better moisturizing effect, but also can help the circulation and transportation of skin moisture, and play a moisturizing and nourishing role in skin care.

[0015] Another object of the present invention is to provide a skin care product, which contains the above-mentioned moisturizing composition and can effectively moisturize, maintain skin health, and improve skin quality.

[0016] To achieve the above object, the present invention provides a moisturizing composition, which comprises, by weight percentage, 50-60% wheat germ extract, 30-45% bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strains, and 1-10% wheat germ oil.

[0017] Preferably, by weight percentage, the wheat germ extract is 55-60%, the filtrate of the bifid yeast fermentation product of wheat germ fermented by bifidobacterium strain is 30-44%, and the wheat germ oil is 1-10%.

[0018] The present invention also provides a skin care product, comprising the above-mentioned moisturizing composition, wherein the content of the moisturizing composition is ≥1%.

[0019] Preferably, the content of the moisturizing composition is 1-5%.

[0020] More preferably, the content of the moisturizing composition can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%.

[0021] Beneficial Effects

[0022] Compared with the prior art, the present invention has at least the following advantages:

[0023] (1) The present invention uses a bifidobacterium strain to ferment wheat germ and obtain a bifidobacterium strain fermented bifidobacterium yeast fermentation product filtrate, which contains a high content of mannose and rare ferulic acid and other small molecular components, which can stimulate the regeneration of skin cells and promote the synthesis of skin water channel proteins, thereby helping the circulation and transportation of skin water, and playing a moisturizing and nourishing role in skin care;

[0024] (2) The present invention uses wheat germ oil as a sealing agent and wheat germ extract as a hygroscopic agent, which can significantly prevent the evaporation of skin moisture and control the moisture content inside the skin. The filtrate of the bifidobacterium fermentation product of wheat germ fermented by bifidobacterium strain can fully balance the moisture content of various parts of the skin, synergistically regulate the moisturizing ability of the skin, and significantly improve the moisturizing effect of the skin.

[0025] (3) The present invention discloses a moisturizing composition that produces synergistic skin moisturizing effects through wheat germ oil, wheat germ extract and the filtrate of the bifid yeast fermentation product obtained by fermenting wheat germ with a bifidobacterium strain. It coordinates internal and external moisturizing from aspects such as water control, water transport to skin external sealing, and can significantly increase the moisture content of the skin. DETAILED DESCRIPTION

[0026] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0027] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0028] In the following examples and comparative examples, wheat germ extract, oat kernel extract and yeast fermentation filtrate of acerola cherry fermentation, yeast fermentation filtrate of wheat germ fermented by yeast, lactic acid bacteria fermentation filtrate of wheat germ fermented by lactic acid bacteria, and oat fermentation filtrate were all purchased from Guangzhou Jianeng Biotechnology Co., Ltd., wheat germ oil and rice bran oil were both purchased from Guangzhou Minghui Fine Chemical Co., Ltd., and bifid yeast fermentation filtrate of wheat germ fermented by bifidobacterium strains was purchased from Angel Yeast Co., Ltd.

[0029] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0030] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight and the % are percentages by weight.

[0031] Example 1

[0032] A moisturizing composition comprises 50 parts of wheat germ extract, 40 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and 10 parts of wheat germ oil.

[0033] Example 2

[0034] A moisturizing composition comprises 60 parts of wheat germ extract, 30 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and 10 parts of wheat germ oil.

[0035] Example 3

[0036] A moisturizing composition comprises 55 parts of wheat germ extract, 44 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and 1 part of wheat germ oil.

[0037] Example 4

[0038] A moisturizing composition comprises 55 parts of wheat germ extract, 40 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and 5 parts of wheat germ oil.

[0039] Example 5

[0040] A moisturizing composition comprises 50 parts of wheat germ extract, 45 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and 5 parts of wheat germ oil.

[0041] Comparative Example 1

[0042] A moisturizing composition comprises 66 parts of wheat germ extract and 34 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains.

[0043] Comparative Example 2

[0044] A moisturizing composition comprises 85 parts of wheat germ extract and 15 parts of wheat germ oil.

[0045] Comparative Example 3

[0046] A moisturizing composition comprises 75 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strains and 25 parts of wheat germ oil.

[0047] Comparative Example 4

[0048] A moisturizing composition comprises 55 parts of oat kernel extract, 40 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and 5 parts of wheat germ oil.

[0049] Comparative Example 5

[0050] A moisturizing composition comprises 55 parts of wheat germ extract, 40 parts of lactic acid bacteria fermentation filtrate obtained by fermenting wheat germ with lactic acid bacteria, and 5 parts of wheat germ oil.

[0051] Comparative Example 6

[0052] A moisturizing composition comprises 55 parts of wheat germ extract, 40 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with yeast, and 5 parts of.

[0053] Comparative Example 7

[0054] A moisturizing composition comprises 55 parts of wheat germ extract, 40 parts of oat fermentation filtrate and 5 parts of wheat germ oil.

[0055] Comparative Example 8

[0056] A moisturizing composition comprises 55 parts of wheat germ extract, 40 parts of bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and 5 parts of rice bran oil.

[0057] Performance Testing

[0058] 1. Determination of Aquaporin 3 Content in HMC-WI-094 Cells

[0059] Experimental Principle

[0060] Aquaporin 3 (AQP-3) is a water glycerol channel protein that transports water and glycerol and is expressed in the epidermis and other epithelial tissues. Upregulation of AQP-3 contributes to increased moisture content of dermal tissue, improved skin texture, and increased skin elasticity.

[0061] The experimental reference compares the results of the water channel protein 3 content determination of the test sample with that of the negative control. If the water channel protein 3 content of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain moisturizing effect.

[0062] Experimental setup

[0063] Composition test: The sample was diluted with pure water to a solution with a volume fraction of 4%, and then filtered with a 0.22 μm filter, and the filtrate was collected as the sample mother solution.

[0064] Negative control: basal medium

[0065] Experimental methods

[0066] Cell activity test: Keratinocytes were plated in 96-well plates. After 24 hours, the culture medium was discarded and basal culture medium containing test samples at different concentrations was added. After 24 hours, OD490nm was detected by MTT method and the effect of test samples on keratinocyte activity was analyzed by t-test.

[0067] Determination of cellular aquaporin 3 content: Keratinocytes were plated on 6-well plates and cultured for 24 hours. The culture medium was replaced with basal culture medium containing test samples at different concentrations. After 24 hours, cell lysis solution was added to fully lyse the cells. The cells were transferred to EP tubes and centrifuged. The supernatant was aspirated and the aquaporin 3 content was determined using an ELISA kit to obtain the effect of the test samples on the aquaporin 3 content of keratinocytes.

[0068] Calculation formula

[0069]

[0070] Data analysis

[0071] The statistical analysis software was SPSS, and the comparison between the test sample and the negative control was performed using the independent sample t test. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0072] The experimental results are shown in Table 1;

[0073] Table 1 Cell test data and analysis results of the composition

[0074]

[0075]

[0076] According to the results in Table 1, we can see that:

[0077] According to the data comparison of Examples 1-5 and the negative control group, the present invention adopts the compound of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and wheat germ oil to effectively promote the increase of water channel protein 3 content; and Examples 1-5 also lead to different promotion effects due to the different ratios between the components, indicating that in the technical scheme of the present invention, the ratio of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and wheat germ oil also has a relatively important influence on the promotion rate of water channel protein 3 content.

[0078] According to the data comparison of Example 2 and Comparative Examples 1-3, although the combination of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strains, and wheat germ oil also has a promoting effect on the content of water channel protein 3, the promoting effect is relatively weak; while the present invention adopts the compound of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting bifidobacterium strains, and wheat germ oil to produce a synergistic effect, which has a significant effect on improving the content of water channel protein 3, indicating that wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting bifidobacterium strains, and wheat germ oil are indispensable in the technical scheme of the present invention, and the compound of the three produces a synergistic effect of improving the content of water channel protein 3.

[0079] According to the data comparison of Example 4 and Comparative Examples 4-8, the present invention uses wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strain, and wheat germ oil to produce a synergistic effect of increasing the content of aquaporin 3; wherein Comparative Example 4 uses oat kernel extract to replace wheat germ extract, which has no promoting effect on the content of aquaporin 3, and the reduction of its aquaporin 3 content is within the normal fluctuation range; while Comparative Examples 5 and 6 use other strains to ferment wheat germ to obtain bifid yeast fermentation product filtrate, which has no synergistic effect with wheat germ extract and wheat germ oil, and has no synergistic effect on aquaporin 3. The promoting effect on the content of water channel protein 3 is limited; Comparative Example 7 uses oat fermentation filtrate to replace the filtrate of the bifid yeast fermentation product fermented by wheat germ by bifidobacterium strain, and its effect is further reduced compared with Comparative Examples 5 and 6, indicating that the promoting effect of the oat fermentation filtrate itself on the content of water channel protein 3 is limited; Comparative Example 8 uses rice bran oil to replace wheat germ oil, and the promoting effect on the content of water channel protein 3 is also relatively weak, indicating that the technical scheme of the present invention using wheat germ extract, bifid yeast fermentation product filtrate fermented by wheat germ by bifidobacterium strain, and wheat germ oil is a preferred scheme for promoting the increase of water channel protein 3 content.

[0080] 2. Human moisturizing efficacy test

[0081] Test method: The test site should not use any products (cosmetics or topical drugs) 2 to 3 days before the test, and should not come into contact with water for 1h to 3h. After entering the laboratory, the subjects wiped the inner side of both forearms with dry paper towels, marked 8 test sites, and randomly divided them into 7 sample areas and 1 control area. The sample area was smeared with the test substance, and the control area was used as a control. The test area was 3cm×3cm, exposed for 20min, and the water content of the skin stratum corneum before the test site sample was applied (T0) was tested, and then the water content was calculated at (2.0±0.1)mg / cm 2The test substance was applied in the sample area at a dose of 100 mg / kg. After application, the subjects returned home on their own and the test area was not allowed to come into contact with water. The water content of the stratum corneum of the test area was tested again 30 min (T1), 1 h (T2), 2 h (T3), and 4 h (T4) after application.

[0082] Before the formal test, the subjects must sit quietly in a standard room for at least 20 minutes without drinking water or beverages. Each part is tested at 3 points each time, and the average value is taken. During the test, the subject's forearm is exposed and placed in the test position, and remains relaxed.

[0083] Instruments and equipment and evaluation instructions

[0084] Table 2 Test instrument information

[0085] Instrument Name Model Specifications Skin Moisture Tester Corneometer CM825 Electronic balance Practum 224-1CN

[0086] Water content of skin stratum corneum: Use a skin moisture tester to test the selected area 3 times and record the average value.

[0087] Parameter description: The moisture content of the skin stratum corneum; the larger the measured value, the higher the moisture content of the skin stratum corneum.

[0088] Result determination: After several hours of trial use of the product, the water content of the stratum corneum of the skin in the sample area increased significantly or the water content of the stratum corneum of the skin in the sample area increased significantly compared with the control area, indicating that the test product has skin moisturizing effect.

[0089] Test Results

[0090] SPSS software was used to perform descriptive statistics on each test value. The Shapiro-Wilk Test was used to test the significance of the normal distribution of the data change value. If P>0.05, it was normally distributed, and the paired sample t test was performed, with a significant difference level of α=0.05. If P<0.05, it was non-normally distributed, and the Wilcoxon signed rank test was performed.

[0091] 2.1 Moisturizing efficacy test of serums containing different concentrations of compositions

[0092] According to Table 3, the essence of the moisturizing composition with different concentrations was prepared, and the amount of each substance used was expressed in parts by mass;

[0093] Table 3 Essence formula containing different concentrations of moisturizing composition

[0094]

[0095]

[0096] The blank control and the essences prepared from samples 1-7 were tested for the moisture content of the stratum corneum according to the above test method. The results are shown in Table 4.

[0097] Table 4 Test results and data analysis results of the moisture content of the skin stratum corneum of the essence containing different concentrations of the composition of Example 1

[0098]

[0099]

[0100] According to Table 4, we can see that:

[0101] There was no significant difference in the average skin moisture content change rate between the blank control and sample 1 in the four time periods of T0 (0h)-T4 (4h); the skin moisture content of sample 2 increased significantly during T0 (0h)-T2 (1h), but gradually decreased after T2 (1h), tended to be close to the initial value of the skin, and no longer showed a significant difference; the skin moisture content of sample 3 increased significantly during T0 (0h)-T3 (2h), but gradually decreased after T3 (2h), tended to be close to the initial value of the skin, and no longer showed a significant difference; samples 4-7 had a significant increase in skin moisture content during T0 (0h)-T3 (2h), but gradually decreased after T3 (2h), tended to be close to the initial value of the skin, and no longer showed a significant difference; The change rates of average skin moisture content in the four time periods (0h)-T4(4h) all showed significant differences, but the difference in the change rate of sample 7 relative to sample 6 was not large; this indicates that the blank control and the 0.5% composition did not show obvious skin moisturizing effect, and that significant skin moisturizing effect was shown starting from a concentration of 1.0%, and as the concentration gradually increased from 1.0% to 5.0%, the moisturizing effect became more significant, so a concentration of 1.0-5.0% is more preferred for the composition, and although a composition with a concentration greater than 5.0% has a significant moisturizing effect, the increase in concentration is of little significance.

[0102] It is explained that when the moisturizing composition of the present invention is applied to skin care products, the content of the moisturizing composition is 1-5% which is the best content range. When the content is lower than 1%, the skin care products cannot show obvious skin moisturizing effect, while when the content is higher than 5%, the moisturizing effect is not further improved obviously, but the production cost is increased.

[0103] 2.2 Moisturizing efficacy test of the essence prepared in Example 1-Example 5 and Comparative Example 1-Comparative Example 8 at the same concentration

[0104] According to the formula of sample 3 in Table 2, essences containing 2% concentration of the compositions of Examples 1 to 5 and Comparative Examples 1 to 8 were prepared, and tested according to the above moisturizing efficacy test. The results are shown in Table 5;

[0105] Table 5 Test results and data analysis results of the moisture content of the stratum corneum of the essences of the compositions of Examples 1 to 5 and Comparative Examples 1 to 8 containing 2% concentration

[0106]

[0107]

[0108] According to the data comparison in Table 5:

[0109] Comparison of Examples 1-5 of the present invention with the control group shows that the technical solution of the present invention using a combination of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ with a bifidobacterium strain, and wheat germ oil can significantly improve the moisturizing effect of the skin.

[0110] From the comparison of the data of Comparative Example 1 and Example 2, it can be seen that although the combination of wheat germ extract and bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain also has good skin moisturizing effect, the improvement of its moisturizing effect is significantly different from that of the technical solution of the present invention, and the moisturizing effect gradually decreases after T2 (1h) and the decrease is large, which shows that the wheat germ extract, bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain, and wheat germ oil in the technical solution of the present invention produce a synergistic moisturizing effect, have significant moisturizing effect and have a longer moisturizing effect.

[0111] Comparison of the data of Example 2 with Comparative Examples 2 and 3 shows that the present invention uses a compound of wheat germ extract, a filtrate of a bifidobacterium-fermented yeast fermentation product obtained by fermenting wheat germ with a bifidobacterium strain, and wheat germ oil to produce a synergistic effect of improving the moisturizing effect, and its moisturizing effect is significantly improved compared to the combination of the three substances in pairs.

[0112] By comparing the data of Example 4 and Comparative Examples 5-8, it can be seen that other components cannot replace one of the components of wheat germ extract, bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strains, and wheat germ oil to enhance the moisturizing effect of the skin; in particular, from the data of Comparative Examples 5 and 6, it can be seen that the skin moisturizing effect that can be produced by the combination of wheat germ bifid yeast fermentation product fermented by different strains with wheat germ extract and wheat germ oil is also significantly different.

[0113] 3. Skin moisture balance test

[0114] Test method: 175 volunteers were recruited and divided into 5 groups, 35 in each group. Each person divided the facial area from the forehead to the chin and from top to bottom into 10 areas. Each area was measured at a certain distance. The same group of people conducted the trial, and different samples were used for the left and right faces (as shown in Table 6). The skin moisture tester was used to test the stratum corneum moisture content of each subject in 10 areas. The initial value of the moisture content of each area was measured 5 times, and then the average value of each area was calculated. The results are shown in Table 6.

[0115] Table 6: Moisture balance test results of the essence containing the composition of Example 1, Example 4 and Comparative Examples 1-8 at a concentration of 2%;

[0116]

[0117]

[0118]

[0119] According to the data results in Table 6:

[0120] According to the comparison of the moisture balance test results of Example 2 and Comparative Examples 1 and 2, it can be seen that the compound of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and wheat germ oil in the present invention can effectively improve the moisture balance of various areas of the skin, make the moisture circulation of the skin more uniform, and comprehensively care for the skin; while the combination of wheat germ extract and bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain, and the combination of wheat germ extract and wheat germ oil in Comparative Examples 1 and 2 cannot effectively improve the moisture circulation of various areas of the skin; the array variance after use and the array variance before use of Comparative Example 1 show that the combination of wheat germ extract and bifid yeast fermentation product filtrate obtained by fermenting wheat germ by bifidobacterium strain has a weak effect of improving the moisture balance of various areas of the skin, while the array variance after use and the array variance before use of Comparative Example 2 show that the combination of wheat germ extract and wheat germ oil has no effect of improving the moisture balance of various areas of the skin.

[0121] The moisture balance test result of Comparative Example 3 is significantly improved compared with that of Example 2. The reason is that the weight percentage of the bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain used in Comparative Example 3 is much greater than that of Example 2, and the bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain is the main functional component for improving the moisture balance of various regions of the skin. Therefore, the array variance of Comparative Example 3 shows that the moisture balance of various regions of the skin is slightly better than that of Example 2; but it can also be found that although the weight percentage of the bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain used in Comparative Example 3 is more than twice that of Example 2, the effect of balancing the moisture of various regions of the skin that can be achieved is not significantly improved compared with Example 2, indicating that the present invention uses wheat germ extract, bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain, and wheat germ oil to produce a synergistic effect of improving the moisture balance of various regions of the skin, and wheat germ extract and wheat germ oil synergistically improve the effect of the bifid yeast fermentation product filtrate of wheat germ fermented by bifidobacterium strain on balancing the moisture of various regions of the skin.

[0122] According to the comparison of the moisture balance test results of Example 4 and Comparative Examples 5 and 6, it can be seen that the lactic acid bacteria fermentation filtrate obtained by fermenting wheat germ by lactic acid bacteria and the bifid yeast fermentation product filtrate obtained by fermenting wheat germ by yeast using Comparative Examples 5 and 6, combined with wheat germ extract and wheat germ oil, all have a certain effect of improving the moisture balance of various areas of the skin. However, compared with the technical scheme of the present invention using the wheat germ extract, the bifid yeast fermentation product filtrate obtained by fermenting wheat germ by a bifidobacterium strain, and wheat germ oil, the improvement effect is not obvious.

[0123] According to the comparison of the moisture balance test results of Example 4 and Comparative Examples 4, 7 and 8, it can be seen that the technical solution of replacing wheat germ extract with oat kernel extract, replacing the filtrate of bifid yeast fermentation product obtained by fermenting wheat germ with bifidobacterium strain with oat fermentation filtrate, and replacing wheat germ oil with rice bran oil cannot effectively improve the moisture balance of various areas of the skin, indicating that the present invention adopts the combination of wheat germ extract, bifid yeast fermentation product filtrate obtained by fermenting wheat germ with bifidobacterium strain, and wheat germ oil to produce a synergistic effect of improving the moisture balance of various areas of the skin.

[0124] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A moisturizing composition, characterized in that: Calculated by weight percentage, it consists of 50-60% wheat germ extract, 30-45% filtrate of bifid yeast fermentation product obtained by fermenting wheat germ with bifidobacterium strains, and 1-10% wheat germ oil.

2. The moisturizing composition according to claim 1, characterized in that By weight percentage, the wheat germ extract is 55-60%, the bifid yeast fermentation product filtrate of the wheat germ fermented by the bifidobacterium strain is 30-44%, and the wheat germ oil is 1-10%.

3. A skin care product, characterized in that: The invention comprises the moisturizing composition as claimed in claim 1 or 2, wherein the content of the moisturizing composition is ≥ 1%.

4. The skin care product according to claim 3, characterized in that: The content of the moisturizing composition is 1-5%.

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