Modular integrated finger press quantitative fluid driving chip for instant detection

Abstract

The application provides a modular integrated finger pressure quantitative fluid driving chip for instant detection, which comprises a batch production method of modular integrated micro valve on the chip, a reusable finger pressure driving module for quantitatively driving fluid volume and a design of microfluidic chip for instant detection. The main material of the micro valve is silicone rubber, and a non-stick film is used as the valve body; the finger pressure driving module comprises a pressing part, a supporting part, a spring and a screw with a pressing head; the instant detection microfluidic chip comprises a three-layer structure, the first layer is used for observing the detection result, the second layer is used for constructing a fluid channel, and the third layer is used for sampling and constructing a micro pump with the micro valve. The micro valve and the finger pressure driving module are combined on the instant detection microfluidic chip, so that the mass production of low-cost micro valves is realized, the quantitative driving of fluid is realized by pressing the finger pressure driving module on the chip, the flow control precision of the micro fluid is ensured, and the accuracy of the chip on-site detection result is improved.

Description

Technical Field

[0001] This invention relates to the field of microfluidic chip technology, and in particular to a modularly integrated finger pressure quantitative fluid drive chip for real-time detection. Background Technology

[0002] Microfluidic chip technology utilizes micro- and nanofabrication techniques to fabricate tiny chips that control fluid flow in micrometer-scale channels, enabling the processing and analysis of minute samples. It holds immense potential in biomedicine, chemical analysis, and environmental monitoring, and has become an indispensable tool in laboratory analysis and biomedical research. However, traditional microfluidic chip systems are complex, inconvenient to carry, costly, and slow to respond, failing to meet the requirements for real-time on-site detection. Therefore, research on the driving and control of microfluidics is essential. This invention focuses on chip-integrated micropump technology and fluid driving control methods for microfluidic chips.

[0003] Chip-integrated micropump technology provides a wealth of methods and tools for research and applications in the field of microfluidics. Micropump types include electrically driven, electrochemically driven, and pressure-driven micropumps. Electrically driven micropumps can precisely control flow and pressure, but require more complex circuitry and control systems. Electrochemically driven micropumps have a compact structure and high mobility, but their response speed is slower and may be limited by bubble generation and chemical reactions in some applications. Pressure-driven micropumps have a simple structure, are easier to modularly integrate onto chips, and meet the driving conditions for high-throughput fluids.

[0004] In finger-pressure driven microfluidic chips, flow control methods for pressure-driven micropumps include direct pressing, indirect control, and injection control. Direct pressing ensures a simple chip structure but can cause significant volumetric errors due to individual finger characteristics (finger force, pressing position). Indirect control, utilizing methods such as pressure transmission through air layers, energy conversion via piezoelectric elements, and capillary force-based finger pressure control, increases chip complexity while maintaining a certain level of driving accuracy and also limits the type of driving fluid. Injection control offers high driving accuracy but requires high pressing sensitivity, and the chip involves complex fabrication processes. Summary of the Invention

[0005] In view of this, the purpose of this invention is to provide a modularly integrated finger-pressure quantitative fluid drive chip for real-time detection. By modularly integrating microvalves on the chip, the chip's portability is improved, the manufacturing difficulty and processing cost of the microvalves are reduced, and fluid actuation no longer relies on bulky external pumps / power supplies or other external devices for input. The reusable finger-pressure drive module for quantitatively driving fluid volume simplifies fluid actuation while ensuring the accuracy of fluid flow control within the chip. By integrating microvalves into the chip to construct a micropump using chip-integrated micropump technology, and using the finger-pressure drive module for fluid actuation, the designed microfluidic chip, through the combination of these two modules, can achieve simple, rapid, and high-precision real-time on-site detection.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a modularly integrated finger pressure quantitative fluid drive chip for real-time detection, comprising a modularly integrated microvalve manufactured with low-cost standardized processing and an adjustable quantitative finger pressure drive module; the modularly integrated microvalve is embedded in the finger pressure quantitative fluid drive chip, and multi-fluid quantitative drive on the chip is realized by pressing the adjustable quantitative finger pressure drive module with a finger.

[0007] In a preferred embodiment, the modular integrated microvalve achieves low-cost, standardized manufacturing through the following steps:

[0008] Step 1: Pour the first layer of silicone rubber raw material (1) onto the first mold (2) and vacuum to eliminate air bubbles; wherein, a large number of semi-circular ring structures (16) are arrayed on the first mold (2).

[0009] Step 2: Place the cover plate (3) on the first mold (2) and the first layer of silicone rubber material (1) so as to flatten the first layer of silicone rubber material (1) to the same height as the arrayed semi-circular ring structure (16); under thermosetting conditions, the first layer of silicone rubber material (1) is cured by vacuuming.

[0010] Step 3: Peel off the cover plate (3), and place the fixed mold (4) flat on the cured first layer of silicone rubber material; put the anti-stick film (5) into the hollowed-out round holes of the fixed mold (4) so ​​that it covers the cured first layer of silicone rubber material and the semi-circular structure (16), and the anti-stick film (5) serves as the valve body part of the micro valve;

[0011] Step 4: Remove the fixed mold (4), place the second mold (6) on the cured first layer of silicone rubber material as shown in Step 4, pour the second layer of silicone rubber material (101) to a certain height, and then put the third mold (7) into the poured second layer of silicone rubber material (101);

[0012] Step 5: Under thermosetting conditions, the second layer of silicone rubber material (101) is cured by vacuuming, and finally the third mold (7), the second mold (6) and the first mold (2) are peeled off in sequence.

[0013] Step 6: Use tools to cut and obtain a single complete check valve (8).

[0014] In a preferred embodiment, the first mold (2) includes a bottom layer and an array of semi-circular ring structures (16) distributed on the bottom layer; the fixed mold (4) is provided with an array of hollowed-out circular holes that cooperate with the anti-stick film (5); the second mold (6) is a cylindrical barrel whose inner diameter determines the outer dimensions of the one-way valve (8); the third mold (7) includes a top layer and an array of cylinders distributed on the top layer; in step 3, when the fixed mold (4) is placed flat on the cured first layer of silicone rubber material, the horizontal projection of the edge of the hole in the fixed mold (4) is located outside the circumference of the horizontal projection of the outer edge of the semi-circular ring structure (16) and has a gap, so that the anti-stick film (5) can be embedded in the one-way valve (8) as the valve body of the one-way valve; when the anti-stick film (5) is placed in the fixed mold (4), the edge of the anti-stick film (5) should coincide with the edge of the hole in the fixed mold (4).

[0015] In a preferred embodiment, the semi-circular ring structure (16), the anti-stick film (5), and the third mold (7) are coaxial.

[0016] In a preferred embodiment, the bottom layer of the first mold (2) and the fixed mold (4) are both circular and have the same diameter.

[0017] In a preferred embodiment, the adjustable quantitative finger pressure drive module includes a pressing part (9), a spring (10), a support part (11), and a screw (12). The pressing part (9) has a threaded hole (17) and a spring mounting hole (18) in the middle to accommodate the screw (12) and the spring (10). The support part (11) has a spring mounting hole (18) corresponding to the pressing part (9) to accommodate the spring (10). The screw (12) has a rotary valve (19) at the top and a pressing head (20) at the bottom. When finger force is applied... When the finger is pressed (9), the spring (10) is compressed. Further, when the finger is pressed to the bottom, the spring (10) reaches its maximum compression and is then limited by the support part (11). This ensures that the finger pressure drive module can maintain the same pressing depth each time it is pressed, thus achieving quantitative driving of the fluid. The screw (12) can be adjusted by rotating the rotary valve (19) to change the finger pressure stroke and adjust the fluid volume driven by a single press, thus realizing the adjustable function of the adjustable quantitative finger pressure drive module. The screw (12) can be equipped with multiple pressing heads (20), and multiple fluids can be driven simultaneously by one press, thus realizing one source and multiple drives.

[0018] In a preferred embodiment, the threaded hole (17) is a through hole; the spring mounting hole (18) provided in the support portion (11) and the pressing portion (9) has the same size; the maximum pressing depth position refers to the position when the upper surface of the screw (12) coincides with the lower surface of the support portion (11); the pressing portion (9), the support portion (11), and the screw (12) are coaxial.

[0019] In a preferred embodiment, the finger pressure quantitative fluid drive chip comprises a three-layer structure: a bottom layer (13), a first PDMS layer (14), and a second PDMS layer (15); the bottom layer (13) is used to observe the instantaneous detection results, the first PDMS layer (14) has flow channels and fluid processing units for instantaneous detection, and is keyly provided with multiple one-way valve holes (24), the one-way valve holes (24) being embedded with one-way valves processed by the low-cost method of the modular integrated microvalve through interference fit; unlike the first PDMS layer (14), the second PDMS layer (15) also has a pressure drive chamber (25), in which fluid... The fluid sample flows in through the inlet (21) of the second layer PDMS (15). By pressing the press head (20) in the finger pressure drive module, the force is transmitted to the pressure drive chamber (25) of the second layer PDMS (15). The pump membrane of the pressure drive chamber (25) vibrates, causing the embedded one-way valve (8) to selectively open or close. After the finger repeatedly presses the press part (9), the fluid sample enters the mixing channel (22) of the first layer PDMS (14) through the micro valve for thorough mixing. Finally, the mixed fluid flows to the detection port (23) of the first layer PDMS (14) for on-site real-time detection.

[0020] In a preferred embodiment, the injection port (21), detection port (23), and one-way valve port (24) are all through holes to facilitate the embedding of the one-way valve (8) and the injection and detection of sample reagents; the pressure drive chamber (25) has a certain distance from the top of the second layer PDMS (15); before pressing the finger pressure drive module, the lower surface of the support part (11) coincides with the upper surface of the second layer PDMS (15), and the pressing head (20) is coaxial with the pressure drive chamber (25).

[0021] In a preferred embodiment, a single pressure-driven chamber (25) connected to two check valves (8) can be configured as a micropump unit (26); the two check valves (8) are placed opposite each other and integrated on the first layer of PDMS (14) of the chip.

[0022] Compared with existing technologies, this invention has the following advantages: This invention provides a modularly integrated finger-pressure quantitative fluid drive chip for real-time detection, achieving modular and mass production of microvalves on the chip while ensuring the flow control accuracy of the fluid within the chip. The microvalves consist of two parts: a material and a valve body. The main material is silicone rubber, with an anti-stick membrane as the valve body. Two microvalves, one in front and one in back, are embedded in the chip, easily forming a micropump unit with the pressure-driven chamber on the chip. The finger-pressure drive module is used to quantitatively drive the fluid. Only finger force is needed to apply to the finger-pressure drive module, ensuring the same pressing depth each time the pressure reaches its limit, thus guaranteeing the fluid volume driven each time and greatly improving the flow control accuracy. The design of the rotary valve at the screw tip can be used to adjust the pressing depth, achieving quantitatively adjustable driving fluid volume. Through the combination of the two modules, the designed microfluidic chip can achieve simple, fast, and high-precision real-time detection on-site. Modular and mass production of the microvalves is achieved, thereby reducing the manufacturing difficulty and processing cost of the microvalves. The miniaturized size of the microvalves allows for relatively simple integration into the microfluidic chip to build a micropump unit. The finger pressure actuation module can quantitatively drive fluid volume, exhibiting high flow control accuracy in fluid actuation. The quantitative adjustment of the driven fluid volume allows for determination of the optimal pressing depth, enabling more efficient on-site detection. The microvalve and finger pressure actuation module are highly reusable and can be applied to other microfluidic chips. Attached Figure Description

[0023] Figure 1 A flowchart illustrating a preferred embodiment of the method for mass production of modularly integrated microvalves on a chip according to the present invention;

[0024] Figure 2 This is an assembly diagram of a reusable finger pressure drive module for quantitatively driving fluid volume according to a preferred embodiment of the present invention.

[0025] Figure 3 This is an assembly diagram of a real-time detection microfluidic chip according to a preferred embodiment of the present invention;

[0026] Figure 4 This is a preferred embodiment of the present invention. Figure 1 A schematic diagram of the structure of the first mold in the middle;

[0027] Figure 5 This is a preferred embodiment of the present invention. Figure 2 A schematic diagram of the pressing and supporting parts;

[0028] Figure 6 This is a preferred embodiment of the present invention. Figure 2 A schematic diagram of the screws in the diagram;

[0029] Figure 7 This is a preferred embodiment of the present invention. Figure 3Top view of the first and second layer PDMS in the middle;

[0030] Figure 8 The diagram shows a finger pressure driving module, a microvalve integrated into a chip, and a cross-sectional view of a micropump unit, which are preferred embodiments of the present invention. Detailed Implementation

[0031] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0032] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0033] It should be noted that the terminology used herein is for the purpose of describing particular implementations only and is not intended to limit the exemplary implementations according to this application; as used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise; furthermore, it should be understood that when the terms “comprising” and / or “including” are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0034] First, such as Figure 1 and Figure 4 As shown, this embodiment of the invention provides a method for the mass production of modularly integrated microvalves on a chip, including the following steps:

[0035] Step 1: Pour the first layer of silicone rubber material 1 onto the first mold 2 and vacuum the mold to eliminate air bubbles; wherein, a large number of semi-circular ring structures 16 are arrayed on the first mold 2; wherein, the first layer of silicone rubber material 1 can be, but is not limited to, PDMS material. The first mold 2 can be, but is not limited to, a glass mold.

[0036] Step 2: Place the cover plate 3 on the first mold 2 and the first layer of silicone rubber material 1, such that the first layer of silicone rubber material 1 is flattened to the same height as the arrayed semi-circular ring structure 16. Under thermosetting conditions, the first layer of silicone rubber material 1 is cured by vacuuming. The cover plate 3 may be, but is not limited to, a metal sheet. The thermosetting conditions may be, but are not limited to, 90°C.

[0037] Step 3: Peel off the cover plate 3 and place the fixing mold 4 flat on the cured first layer of silicone rubber material. Place the anti-stick film 5 into the array of perforated circular holes in the fixing mold 4, so that it covers the cured first layer of silicone rubber material and the semi-circular structure 16. The anti-stick film 5 serves as the valve body part of the micro-valve. The fixing mold 4 can be, but is not limited to, a glass mold. The anti-stick film 5 can be, but is not limited to, a silicon dioxide metal mask.

[0038] Step 4: Remove the fixed mold 4, place the second mold 6 on the cured first layer of silicone rubber material as shown in Step 4, pour the second layer of silicone rubber material (101) to a certain height, and then place the third mold 7 into the poured second layer of silicone rubber material (101). The second layer of silicone rubber material (101) can be, but is not limited to, PDMS material. The second mold 6 and the third mold 7 can be, but are not limited to, glass molds.

[0039] Step 5: Under thermosetting conditions, the second layer of silicone rubber material (101) is cured by vacuuming, and finally the third mold 7, the second mold 6 and the first mold 2 are peeled off in sequence.

[0040] Step 6: Use tools to cut and obtain a single complete one-way valve 8.

[0041] The first mold 2, the second mold 6, and the third mold 7 are not limited to being manufactured using soft photolithography; for example, they can be manufactured using metal materials via 3D printing. The first layer of silicone rubber material 1 and the second layer of silicone rubber material (101) can be replaced with other silicone materials. The anti-stick film 5 can be replaced with other metal or non-metal materials, and the process can be, but is not limited to, chemical vapor deposition.

[0042] like Figure 1 and Figure 4 As shown, in a preferred embodiment, the first mold 2 includes a bottom layer and an array of semi-circular ring structures 16 distributed on the bottom layer; the fixed mold 4 is provided with an array of perforated circular holes that cooperate with the anti-adhesive film 5; the second mold 6 is a cylindrical barrel, the inner diameter of which determines the external dimensions of the one-way valve; the third mold 7 includes a top layer and an array of cylinders distributed on the top layer; in step 3, when the fixed mold 4 is placed flat on the cured first layer of silicone rubber material, the horizontal projection of the edge of the hole in the fixed mold 4 is located outside the circumference of the horizontal projection of the outer edge of the semi-circular ring structure 16 and has a gap, so that the anti-adhesive film 5 can be embedded in the one-way valve 8 as the valve body of the one-way valve. When the anti-adhesive film 5 is placed in the fixed mold 4, the edge of the anti-adhesive film 5 should coincide with the edge of the hole in the fixed mold 4.

[0043] In a preferred embodiment, the semi-circular ring structure 16, the anti-stick film 5, and the third mold 7 are coaxial.

[0044] In a preferred embodiment, the bottom layer of the first mold 2 and the fixed mold 4 are both circular and have the same diameter.

[0045] Secondly, such as Figure 2 , Figure 5 and Figure 6As shown, in a preferred embodiment, the reusable, adjustable pressing stroke, and quantitatively driven fluid volume finger pressure driving module includes four parts: a pressing part 9, a spring 10, a support part 11, and a screw 12. The pressing part 9 has a threaded hole 17 and a spring mounting hole 18 in the middle to facilitate the installation of the screw 12 and the spring 10. The support part 11 has a spring mounting hole 18 corresponding to the pressing part 9 for installing the spring 10. The top and bottom of the screw 12 are each provided with a rotary valve 19 and a pressing head 20. When finger force is applied to the pressing part 9, the spring 10 is compressed. Furthermore, when the finger is pressed all the way down, that is, after the spring 10 reaches its maximum compression, it is limited by the support part 11, so that the finger pressure driving module can ensure the same pressing depth each time it is pressed, thereby realizing the quantitative driving of fluid.

[0046] In a preferred embodiment, the spring 10 and the spring mounting hole 18 may be, but are not limited to, three; the pressing head 20 may be, but are not limited to, four; and the rotary valve 19 may be, but is not limited to, a single-pole valve.

[0047] In a preferred embodiment, the pressing part 9, the spring 10, the supporting part 11, and the screw 12 may be, but are not limited to, made of metal materials.

[0048] In a preferred embodiment, the threaded hole 17 is a through hole; the spring mounting holes 18 provided on the support portion 11 and the pressing portion 9 are of the same size; the maximum pressing depth position refers to the position when the upper surface of the screw 12 coincides with the lower surface of the support portion 11; the pressing portion 9, the support portion 11, and the screw 12 are coaxial.

[0049] Finally, as Figure 3 , Figure 7 and Figure 8As shown, in a preferred embodiment, a finger-pressure driven microfluidic chip is characterized by comprising a three-layer structure: a bottom layer 13, a first PDMS layer 14, and a second PDMS layer 15. The bottom layer 13 is used to observe real-time detection results. The first PDMS layer 14 has an inlet port 21, a mixing channel 22, a detection port 23, and a one-way valve port 24, thus serving as a fluid channel to transport samples from the inlet port to the detection port. Unlike the first PDMS layer 14, the second PDMS layer 15 also has a pressure-driven chamber 25, wherein the flow... The fluid flows in through the inlet 21 of the second layer PDMS15. The finger applies force to the pressure drive chamber 25 of the second layer PDMS15 through the pressing head 20 in the finger pressure drive module. The pump membrane of the pressure drive chamber 25 vibrates, causing the one-way valve 8 to selectively open / close. After the finger repeatedly presses the pressing part 9, the fluid sample enters the mixing channel 22 of the first layer PDMS14 through the micro-valve for thorough mixing. Finally, the mixed fluid flows to the detection port 23 of the first layer PDMS14 for on-site real-time detection.

[0050] In a preferred embodiment, the injection port 21, detection port 23, and one-way valve port 24 are all through holes to facilitate the embedding of the one-way valve 8 and the injection and detection of sample reagents; the pressure-driven chamber 25 has a certain distance from the top of the second layer PDMS 15; before pressing the finger pressure driving module, the lower surface of the support part 11 coincides with the upper surface of the second layer PDMS 15, and the pressing head 20 is coaxial with the four pressure-driven chambers 25; the injection port 21 and pressure-driven chamber 25 may be, but are not limited to, four; the mixing channel 22 and detection port 23 may be, but are not limited to, two; and the one-way valve port 24 may be, but is not limited to, eight.

[0051] In a preferred embodiment, a single pressure-driven chamber 25 is connected to two one-way valves 8 to form a micropump unit 26.

[0052] In a preferred embodiment, the two one-way valves 8 are placed opposite each other and integrated on the first layer PDMS14 of the chip.

[0053] In a preferred embodiment, real-time blood type detection can be performed by integrating the micropump unit 26 and the finger pressure driving module into a chip. The steps are as follows: blood sample, anti-A reagent, and anti-B reagent are injected through the sample inlet 21 respectively. The finger repeatedly presses the finger pressure driving module, driving a quantitative volume of fluid each time, until the blood sample is mixed with the two antibodies in the mixing channel 22. Finally, the reaction mixture is driven to the detection port 23 respectively, and the blood agglutination result can be observed to determine the blood type on the spot. The real-time detection microfluidic chip can be used for, but is not limited to, real-time blood type detection.

[0054] It should be noted that relational terms such as "first" and "second" described in this invention are only used to distinguish an entity or operation, and do not necessarily imply any actual sequential relationship between these entities or operations.

[0055] The background section of this invention may include background information about the problems or environment in which the invention is being developed, but it is not necessarily a description of prior art. Therefore, the content included in the background section does not constitute an admission of prior art by the applicant.

[0056] The above description, in conjunction with specific / preferred embodiments, provides a detailed explanation of the present invention. However, it should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various substitutions or modifications can be made to these described embodiments without departing from the concept of the present invention, and all such substitutions or modifications should be considered within the scope of protection of the present invention.

Claims

1. A modularly integrated finger pressure quantitative fluid drive chip for instantaneous detection, characterized in that... This includes a modular integrated microvalve with low-cost standardized processing and an adjustable quantitative finger pressure drive module; the modular integrated microvalve is embedded in a finger pressure quantitative fluid drive chip, and multi-fluid quantitative drive on the chip is realized by pressing the adjustable quantitative finger pressure drive module with a finger; the adjustable quantitative finger pressure drive module includes a pressing part (9), a spring (10), a support part (11), and a screw (12), the pressing part (9) is provided with a threaded hole (17) and a spring mounting hole (18) in the middle to facilitate the installation of the screw (12) and the spring (10), The support part (11) has a spring mounting hole (18) corresponding to the pressing part (9) for mounting the spring (10). The top and bottom of the screw (12) are each provided with a rotary valve (19) and a pressing head (20). When the finger force is applied to the pressing part (9), the spring (10) is compressed. Furthermore, when the finger is pressed to the bottom, that is, the spring (10) reaches the maximum compression amount and is limited by the support part (11), so that the finger pressure driving module can ensure the same pressing depth each time it is pressed, and realize the quantitative driving of fluid. The screw (12) changes the finger pressure stroke by adjusting the rotary valve (19), thereby adjusting the fluid volume driven by a single press and realizing the adjustable function of the adjustable quantitative finger pressure drive module; the screw (12) can be equipped with multiple pressing heads (20), and a single press can drive multiple fluids at the same time, realizing one source and multiple drives.

2. The modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 1, characterized in that, The modular integrated microvalve is described through the following steps: Step 1: Pour the first layer of silicone rubber raw material (1) onto the first mold (2) and vacuum to eliminate air bubbles; wherein, a large number of semi-circular ring structures (16) are arrayed on the first mold (2). Step 2: Place the cover plate (3) on the first mold (2) and the first layer of silicone rubber material (1) so as to flatten the first layer of silicone rubber material (1) to the same height as the arrayed semi-circular ring structure (16); under thermosetting conditions, the first layer of silicone rubber material (1) is cured by vacuuming. Step 3: Peel off the cover plate (3), and place the fixed mold (4) flat on the cured first layer of silicone rubber material; put the anti-stick film (5) into the hollowed-out round holes of the fixed mold (4) so ​​that it covers the cured first layer of silicone rubber material and the semi-circular structure (16), and the anti-stick film (5) serves as the valve body part of the micro valve; Step 4: Remove the fixed mold (4), place the second mold (6) on the cured first layer of silicone rubber material as shown in Step 4, pour the second layer of silicone rubber material (101) to a certain height, and then put the third mold (7) into the poured second layer of silicone rubber material (101); Step 5: Under thermosetting conditions, the second layer of silicone rubber material (101) is cured by vacuuming, and finally the third mold (7), the second mold (6) and the first mold (2) are peeled off in sequence. Step 6: Use tools to cut and obtain a single complete check valve (8).

3. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 2, characterized in that, The first mold (2) includes a bottom layer and an array of semi-circular ring structures (16) distributed on the bottom layer; the fixed mold (4) is provided with an array of hollowed-out circular holes that cooperate with the anti-stick film (5); the second mold (6) is a cylindrical barrel whose inner diameter determines the outer dimensions of the one-way valve (8); the third mold (7) includes a top layer and an array of cylinders distributed on the top layer; in step 3, when the fixed mold (4) is placed flat on the cured first layer of silicone rubber material, the horizontal projection of the edge of the hole in the fixed mold (4) is located outside the circumference of the horizontal projection of the outer edge of the semi-circular ring structure (16) and has a gap, so that the anti-stick film (5) can be embedded in the one-way valve (8) as the valve body of the one-way valve; when the anti-stick film (5) is placed in the fixed mold (4), the edge of the anti-stick film (5) should coincide with the edge of the hole in the fixed mold (4).

4. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 2, characterized in that, The semi-circular ring structure (16), the anti-stick film (5), and the third mold (7) are coaxial.

5. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 2, characterized in that, The bottom layer of the first mold (2) and the fixed mold (4) are both circular and have the same diameter.

6. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 1, characterized in that, The threaded hole (17) is a through hole; the spring mounting hole (18) provided in the support part (11) and the pressing part (9) has the same size; the maximum pressing depth position refers to the position when the upper surface of the screw (12) coincides with the lower surface of the support part (11); the pressing part (9), the support part (11), and the screw (12) are coaxial.

7. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 1, characterized in that, The finger pressure quantitative fluid drive chip comprises a three-layer structure: a bottom layer (13), a first PDMS layer (14), and a second PDMS layer (15). The bottom layer (13) is used to observe the instantaneous detection results. The first PDMS layer (14) has flow channels and fluid processing units for instantaneous detection, and is keyly equipped with multiple one-way valve holes (24). The one-way valve holes (24) are embedded with one-way valves processed by the low-cost method of the modular integrated microvalve through interference fit. Unlike the first PDMS layer (14), the second PDMS layer (15) also has a pressure drive chamber (25), in which fluid flows from the second PDMS layer. The sample flows into the inlet (21) of the second-layer PDMS (15). By pressing the press head (20) in the finger pressure drive module, the force is transmitted to the pressure drive chamber (25) of the second-layer PDMS (15). The pump membrane of the pressure drive chamber (25) vibrates, causing the embedded one-way valve (8) to selectively open or close. After the finger repeatedly presses the press part (9), the fluid sample enters the mixing channel (22) of the first-layer PDMS (14) through the micro-valve for thorough mixing. Finally, the mixed fluid flows to the detection port (23) of the first-layer PDMS (14) for on-site real-time detection.

8. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 7, characterized in that, The injection port (21), detection port (23), and one-way valve port (24) are all through holes to facilitate the embedding of the one-way valve (8) and the injection and detection of sample reagents; the pressure drive chamber (25) has a certain distance from the top of the second layer PDMS (15); before pressing the finger pressure drive module, the lower surface of the support part (11) coincides with the upper surface of the second layer PDMS (15), and the pressing head (20) is coaxial with the pressure drive chamber (25).

9. A modularly integrated finger pressure quantitative fluid drive chip for real-time detection according to claim 7, characterized in that, A single pressure-driven chamber (25) connected to two check valves (8) can be configured into a micropump unit (26); the two check valves (8) are placed opposite each other and integrated on the first layer of PDMS (14) of the chip.

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