An intake structure installation assembly and a plant tissue culture tank
By designing the installation components of the intake structure, the problem that the aerator cannot flexibly adjust its position is solved, the flexible installation of the intake structure in the culture tank is realized and the aeration effect is fully utilized, and the circulation flow of the culture medium and the rapid growth of uncertain roots is promoted.
Patent Information
- Application Number
- CN202311561274.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-22
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2043-11-22
AI Technical Summary
The aerator of existing plant tissue culture tanks cannot flexibly adjust the position of the aeration pipe and the tank body, resulting in insufficient aeration effect, limiting its application in different environments.
An air intake structure installation assembly is designed, including seat plates, laminates, hub kits and sealing rings. Through the combination of these components, the air intake structure allows the flexibly adjusting position and posture in the plant tissue culture tank, improves the aeration effect, and promotes the circulating flow of the culture medium.
The flexible installation of the air intake structure in the culture tank and the full play of the aeration effect are achieved, the circulation flow of the culture medium is promoted, the indefinite roots are avoided, and the growth rate of the indefinite roots is improved.
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Figure CN117322344B_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of plant tissue culture devices, and specifically relates to an air inlet structure installation assembly and a plant tissue culture tank thereof. Background Art
[0002] In the prior art, technicians have developed a method and a culture device for large-scale cultivation using plant tissues or cells in vitro. By peeling off plant organs such as roots, stems, and leaves, and then placing them in a culture medium containing nutrients for cultivation, while providing environmental conditions suitable for growth such as temperature and oxygen, callus, adventitious buds, and adventitious roots are induced. Finally, using these callus, adventitious buds, and adventitious roots as seeds for cultivating plants, and placing them in a culture device for cultivation.
[0003] In existing plant tissue culture tanks, an aeration device for increasing dissolved oxygen is usually movably placed on the bottom of the tank, or an aeration pipe provided with air outlets is arranged against the tank wall of the culture tank.
[0004] Chinese Utility Model Patent No. 201721605130.3 discloses a new type of disc microporous aerator, which includes a disc bracket and a flexible aeration pipe. The disc bracket includes an annular base, a bracket, and a plurality of support rods. The bracket is arranged above the annular base, and the plurality of support rods are arranged at intervals between the annular base and the bracket. The flexible aeration pipe is arranged on the upper surface of the bracket, and one end of the flexible aeration pipe is connected to the first connection port of a three-way valve, and the other end of the flexible aeration pipe spirally extends from the outermost side of the bracket to the center of the bracket and then is connected to the second connection port of the three-way valve. The third connection port of the three-way valve is connected to an air inlet. Among them, the bracket includes a bracket body and a plurality of reinforcing rods, and the flexible aeration pipe and the reinforcing rods are also locked by a fastening mechanism. The aerator in this patent improves the uniformity and stability of the air outlet of the flexible aeration pipe, and at the same time has a simple and stable structure and is safer to use.
[0005] However, in this aerator, the aeration pipe for ejecting gas is connected to the bracket through a fastening mechanism and is distributed on the upper surface of the bracket. During use, the position of the aeration pipe and the bracket cannot be flexibly adjusted according to the actual environment, which limits the application conditions of the aerator and causes the aerator to not fully exert its aeration effect in actual use.
[0006] In view of this, the present invention is specifically proposed. Summary of the Invention
[0007] The purpose of the present invention is to provide an air inlet structure installation assembly for a plant tissue culture tank, so as to achieve the purpose of flexibly adjusting the relative position and attitude of the air inlet structure and the plant tissue culture tank when installing the air inlet structure.
[0008] Another object of the present invention is to provide a plant tissue culture tank adopting the above-mentioned air inlet structure installation component, so as to improve the aeration effect of the air inlet structure, better promote the circulation of the solution in the tank, and promote the growth of adventitious roots.
[0009] To solve the above technical problems, the basic concept of the technical solution adopted by the present invention is:
[0010] An air inlet structure installation component of a plant tissue culture tank includes an interface opened on the tank wall of the plant tissue culture tank. The air inlet structure includes an air inlet part, an aeration part, and a conduit connecting the air inlet part and the aeration part. It also includes a seat plate that can be embedded at the interface, which is sleeved on the outer periphery of the conduit and connected with the edge of the interface at its edge, separating the aeration part and the air inlet part inside and outside the plant tissue culture tank.
[0011] Furthermore, it also includes an annular pressing member. The pressing member is sleeved on the seat plate and abuts against the plate surface of the seat plate facing the outside of the plant tissue culture tank at the edge of its ring hole, for clamping the air inlet structure on the plant tissue culture tank.
[0012] Furthermore, it also includes a hub kit nested in the interface. The outer peripheral surface of the hub kit is connected with the interface and protrudes axially towards the inside and outside of the tank wall, and its inner peripheral surface is connected with the seat plate.
[0013] Furthermore, one end of the hub kit inside the tank wall of the plant tissue culture tank is provided with a support ring, which extends radially from the inner peripheral surface of the hub kit towards the center. The seat plate is sleeved on the inner peripheral surface of the hub kit from the outside of the tank wall of the plant tissue culture tank and abuts against the support ring.
[0014] Furthermore, it also includes a sealing ring, which is arranged between the seat plate and the support ring and is clamped by the end face of the seat plate facing the inside of the plant tissue culture tank and the support ring.
[0015] Furthermore, the support ring is provided with a groove with an opening facing the outside of the tank wall of the plant tissue culture tank, and the sealing ring is installed in the groove by being extruded by the seat plate.
[0016] Furthermore, the end face of the hub kit protruding outside the tank wall is provided with a connection hole extending along its axis. The pressing member can be connected with the end face of the hub kit to clamp the seat plate and is provided with a through hole corresponding to the connection hole for passing through and fixing screws.
[0017] Furthermore, the inner peripheral surface of the hub kit is a cylindrical surface, the seat plate is spherical and its radius is equal to the radius of the inner peripheral surface of the hub kit. The pressing member and the support ring rotatably clamp the seat plate in the hub kit.
[0018] Further, one end of the overlapping member and the support ring close to the inner peripheral surface of the hub kit is provided with a spherical surface.
[0019] The present invention also provides a plant tissue culture tank using any one of the above intake structure mounting assemblies.
[0020] After adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.
[0021] 1. The mounting assembly includes a seat plate and an interface, and the intake structure is fixedly connected to the seat plate. By rotating the seat plate relative to the interface of the culture tank, the relative position of the aeration part of the intake structure in the culture tank can be adjusted, so that the intake structure can have more relative positions and postures relative to the culture tank, improving the flexibility of the installation and setting of the intake structure, and also being beneficial to giving full play to the aeration effect of the intake structure in the culture tank.
[0022] 2. By setting the overlapping member, the seat plate can rotate at any angle relative to the interface, so that a large number of small bubbles ejected from the intake structure can be dispersed in the solution, which is beneficial to promoting the circulation of the solution and improving the dissolved oxygen effect, and can better promote the circulation of the culture solution in the culture tank, avoid the entanglement and agglomeration of adventitious roots, and thus promote the faster growth of adventitious roots.
[0023] 3. By setting a groove on the end face of the support ring and fixing the sealing ring in the groove, when the sealing ring is squeezed and deformed, it can obtain rigid support, avoid being damaged by huge pressure, improve the service life of the sealing ring, and at the same time prevent the sealing ring from slipping out of the set position after being squeezed, causing the problem of sealing failure.
[0024] 4. The seat plate is connected to the tank wall of the culture tank through the clamping of the overlapping member and the hub kit, so that the intake structure passing through and fixed from the center of the seat plate has more positions in the culture tank. When installing the intake structure, the seat plate can rotate at any angle relative to the hub kit, and by rotating the seat plate, the posture and position of the aeration part can be changed, which greatly facilitates the installation of the intake structure and makes the mounting assembly more flexible.
[0025] The following further describes in detail the specific embodiments of the present invention with reference to the drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] The drawings, as a part of the present invention, are used to provide a further understanding of the present invention. The schematic embodiments and descriptions thereof of the present invention are used to explain the present invention, but do not constitute an improper limitation of the present invention. Obviously, the drawings described below are only some embodiments, and those of ordinary skill in the art can obtain other drawings according to these drawings without creative efforts. In the drawings:
[0027] Figure 1 It is a schematic diagram of an air intake structure installation assembly of a plant tissue culture tank of the present invention;
[0028] Figure 2 It is a schematic diagram of an air intake structure of a plant tissue culture tank of the present invention;
[0029] Figure 3 It is a cross-sectional schematic diagram of an air intake structure in the present invention.
[0030] Among them: 10, plant tissue culture tank; 11, tank wall; 40, air intake structure; 41, air intake part; 42, aeration part; 421, cavity; 43, conduit; 431, air intake section; 432, air outlet section; 433, transition section; 44, seat plate; 45, quick-release joint; 451, sealing groove; 51, interface; 52, laminated part; 521, through hole; 53, hub kit; 531, support ring; 532, groove; 533, connecting hole; 54, sealing ring.
[0031] It should be noted that these drawings and textual descriptions are not intended to limit the conceptual scope of the present invention in any way, but are intended to illustrate the concept of the present invention for those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION
[0032] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. The following embodiments are used to illustrate the present invention but are not used to limit the scope of the present invention.
[0033] In the description of the present invention, it should be noted that the terms "inside", "outside", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
[0034] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "contacted", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0035] In the present invention, plant tissues include callus tissues, adventitious buds, adventitious roots, etc. induced by using tissues or cells isolated from plants.
[0036] A culture device for large-scale cultivation of adventitious roots is provided with at least two plant tissue culture tanks of unequal volumes.
[0037] Taking the culture device with two plant tissue culture tanks as an example. For the convenience of distinction, the plant tissue culture tank with a smaller volume is called the adventitious root seed tank, and the plant tissue culture tank with a larger volume is called the adventitious root fermentation tank.
[0038] Among them, the tank body of the plant tissue culture tank is entirely made of stainless steel material. The ratio of the height to the diameter of the tank body is 1:1. The volume of the adventitious root seed tank is 200L, and the volumes of the adventitious root fermentation tanks are 1000L and 1500L.
[0039] The plant tissue culture tank is filled with culture solution, and the growth rate of adventitious roots in the plant tissue culture tank is much faster than that of primary roots.
[0040] Example 1
[0041] As Figure 1 shown, in the embodiment of the present invention, an air inlet structure installation assembly of a plant tissue culture tank 10 is introduced. This installation assembly can make the connection process between the air inlet structure 40 and the plant tissue culture tank 10 more convenient.
[0042] Among them, the air inlet structure 40 includes an air inlet part 41, an aeration part 42, and a conduit 43 connecting the air inlet part 41 and the aeration part 42. The conduit 43 is set in a straight rod shape or a shape of multiple straight rods bent, and the air inlet part 41 and the aeration part 42 are respectively connected to both ends of the conduit 43.
[0043] Specifically, the air inlet structure 40 installation assembly includes an interface 51 opened on the tank wall 11 of the plant tissue culture tank 10, and also includes a seat plate 44 that can be embedded at the interface 51. The seat plate 44 is sleeved on the outer periphery of the conduit 43 and is connected to the edge of the interface 51 with its outer peripheral edge, separating the aeration part 42 from the air inlet part 41 inside and outside the plant tissue culture tank 10.
[0044] Preferably, the outer peripheral edge of the seat plate 44 is provided with an external thread, and the corresponding edge of the interface 51 is provided with an internal thread. The seat plate 44 and the interface 51 are connected together by the engagement of the internal and external threads.
[0045] In this embodiment, the installation assembly includes a seat plate 44 and an interface 51. The air inlet structure 40 is fixedly connected to the seat plate 44. By rotating the seat plate 44 relative to the interface 51 of the culture tank, the relative position of the aeration part 42 of the air inlet structure 40 inside the culture tank can be adjusted, so that the air inlet structure 40 can have more relative positions and postures relative to the culture tank, improving the flexibility of the installation and setting of the air inlet structure 40, and also being beneficial to giving full play to the aeration effect of the air inlet structure 40 inside the culture tank.
[0046] As Figure 1 shown, in one embodiment of the present invention, an intake structure 40 mounting assembly is introduced which can rotate at any angle around its own central axis when connected to a culture tank. The mounting assembly includes an annular stacking member 52.
[0047] When fixedly installing the intake structure 40, the stacking member 52 and the interface 51 on the culture tank are connected by end faces and the seat plate 44 is clamped between the two. Thus, when the stacking member 52 and the interface 51 are not in close contact, the seat plate 44 can be rotated to adjust the position and attitude of the intake structure 40 relative to the culture tank.
[0048] Specifically, the stacking member 52 is set as an annular structure with a central ring hole. The stacking member 52 is sleeved with the seat plate 44 and its edge of the ring hole abuts against the plate surface of the seat plate 44 facing the outside of the plant tissue culture tank 10, for clamping the intake structure 40 on the plant tissue culture tank 10.
[0049] In this embodiment, by setting the stacking member 52, the seat plate 44 can rotate at any angle relative to the interface 51. Furthermore, a large number of small bubbles ejected from the intake structure 40 can be dispersed in the solution, which is beneficial to promoting the circulation of the solution and improving the dissolved oxygen effect, can better promote the circulation of the culture solution in the culture tank, avoid the entanglement and agglomeration of adventitious roots, and thus promote the faster growth of adventitious roots.
[0050] As Figure 1 shown, in one embodiment of the present invention, a mounting assembly is introduced which can clamp and fix the intake structure 40 more firmly.
[0051] Specifically, the mounting assembly further includes a hub kit 53 with a cylindrical outer peripheral surface. The hub kit 53 is nested in the interface 51 and its outer peripheral surface is connected to the interface 51. Particularly, the axis of the hub kit 53 is perpendicular to the surface of the tank wall 11 of the culture tank and protrudes towards the inside and outside of the tank wall 11 along its own axis.
[0052] At the same time, the inner peripheral surface of the hub kit 53 forming a central hole is connected to the seat plate 44.
[0053] In this embodiment, the protrusion of the hub kit 53 from the tank wall 11 of the culture tank increases the width of the contact surface between the seat plate 44 and the hub kit 53, and has higher sealing performance and greater connection strength compared with the direct connection between the seat plate 44 and the tank wall 11.
[0054] As Figure 1 shown, in one embodiment of the present invention, a hub kit 53 is introduced which can wrap the seat plate 44 after optimization and improvement.
[0055] Specifically, one end of the hub kit 53 facing the inner side of the tank wall 11 of the plant tissue culture tank 10 is provided with a support ring 531. The support ring 531 extends radially from the inner circumferential surface of the hub kit 53 towards the central axis of the hub kit 53, and forms a through hole 521 that can pass through the aeration part 42.
[0056] The seat plate 44 is sleeved on the inner circumferential surface of the hub kit 53 from the outside of the tank wall 11 of the plant tissue culture tank 10, and the seat plate 44 abuts against the support ring 531.
[0057] In this embodiment, by setting the support ring 531, it is ensured that the seat plate 44 sleeved into the hub kit 53 can withstand a greater pressing force, and it is avoided that the seat plate 44 falls off the hub kit 53 into the plant tissue culture tank 10 when it is under a greater pressure. Thus, setting the support ring 531 on the hub kit 53 not only improves the stability of the installation assembly of the air intake structure 40, but also improves the sealing performance of the installation assembly of the air intake structure 40.
[0058] As Figure 1 shown, in an embodiment of the present invention, an installation assembly of an air intake structure 40 with better sealing performance is introduced. The installation assembly of the air intake structure 40 further includes a sealing ring 54 for filling the gaps between the contact surfaces.
[0059] Specifically, the sealing ring 54 is made of rubber material, has elasticity and good ductility. And, the sealing ring 54 is arranged between the seat plate 44 and the support ring 531, and is clamped by the end face of the seat plate 44 facing the inside of the tank of the plant tissue culture tank 10 and the end face of the support ring 531.
[0060] Particularly, when the seat plate 44 and the support ring 531 clamp the sealing ring 54, the thickness of the sealing ring 54 is reduced, so that the convex and concave on the end faces of the seat plate 44 and the support ring 531 close to each other are filled by the sealing ring 54, improving the sealing performance of the installation assembly of the air intake structure 40.
[0061] As Figure 1 shown, in an embodiment of the present invention, an installation assembly of an air intake structure 40 that can better define the sealing ring 54 is introduced.
[0062] Specifically, the support ring 531 is arranged at the end of the hub kit 53 located inside the tank wall 11. The through hole 521 on the support ring 531 is arranged coaxially with the central axis of the central hole of the hub kit 53. And, one end face of the support ring 531 is flush with the end face of the hub kit 53, and the other is located in the central hole of the hub kit 53.
[0063] Specifically, a groove 532 is formed on the end face of the support ring 531 located within the central hole of the hub assembly 53. The opening of the groove 532 faces the outside of the wall 11 of the plant tissue culture tank 10. The sealing ring 54 is embedded in the groove 532 and protrudes from the end face of the support ring 531. When installing and fixing the air intake structure 40, the seat plate 44 presses the sealing ring 54, causing the sealing ring 54 to deform and fill the groove 532 completely.
[0064] In this embodiment, by providing the groove 532 on the end face of the support ring 531 and fixing the sealing ring 54 in the groove 532, the sealing ring 54 can be rigidly supported when being extruded and deformed, preventing it from being damaged by huge pressure, improving the service life of the sealing ring 54. At the same time, it can also prevent the sealing ring 54 from slipping out of the set position after being extruded, thus avoiding the problem of sealing failure.
[0065] As Figure 1 shown, in an embodiment of the present invention, an air intake structure 40 installation assembly with better flexibility is introduced. The seat plate 44 of this installation assembly is sleeved and fitted with a clearance to the hub assembly 53. The seat plate 44 can be flexibly rotated when inserted into the central hole of the hub assembly 53, and the seat plate 44 is clamped and fixed in the hub assembly 53 by extrusion from the other side of the seat plate 44 through the stacking member 52.
[0066] Specifically, a connection hole 533 is provided on the end face of the hub assembly 53 protruding from the outside of the wall 11 of the culture tank. The connection hole 533 extends along the axial direction of the hub assembly 53 and is used to fix the stacking member 52.
[0067] Particularly, the stacking member 52 is connected to the hub assembly 53 with end faces, clamping the seat plate 44 in the middle. And the stacking member 52 is provided with a through hole 521 corresponding to the connection hole 533 for passing through and fixing screws.
[0068] In this embodiment, the seat plate 44 is connected to the wall 11 of the culture tank through the clamping of the stacking member 52 and the hub assembly 53, enabling the air intake structure 40 passed through and fixed from the center of the seat plate 44 to have more positions in the culture tank. When installing the air intake structure 40, the seat plate 44 can rotate at any angle relative to the hub assembly 53, and by rotating the seat plate 44, the attitude and position of the aeration part 42 can be changed, greatly facilitating the installation of the air intake structure 40 and making the installation assembly more flexible.
[0069] As Figure 1 shown, in an embodiment of the present invention, a more flexible air intake structure 40 installation assembly is introduced.
[0070] The inner peripheral surface of the hub kit 53 is a cylindrical surface. The seat plate 44 is spherical and has the same radius as the inner peripheral surface of the hub kit 53. The pressing member 52 and the support ring 531 rotatably clamp the seat plate 44 in the hub kit 53.
[0071] In another embodiment, in order to better apply a force to the seat plate 44 and evenly distribute the force over the entire cross-section of the seat plate 44, one end of the pressing member 52 and the support ring 531 close to the inner peripheral surface of the hub kit 53 is provided with a spherical curved surface.
[0072] In this embodiment, the seat plate 44 is made spherical, and the end surfaces of the pressing member 52 and the support ring 531 that contact the seat plate 44 are hemispherical curved surfaces corresponding to the seat plate 44, which can enable the air intake structure 40 to rotate flexibly in more directions, and can also avoid the problem of stress concentration of the force acting on the seat plate 44, thereby improving the service life of the installation assembly.
[0073] The present invention also provides a plant tissue culture tank 10 employing the installation assembly of any one of the above air intake structures 40.
[0074] Specifically, the plant tissue culture tank 10 has an inverted conical tank bottom, which can reduce the pressure of the nutrient solution on the adventitious roots and is beneficial to the growth of adventitious roots. An interface 51 for connecting the air intake structure 40 is provided on the conical side wall. The air intake structure 40 installation assembly is arranged at the position of the interface 51.
[0075] In this embodiment, the air intake structure 40 installation assembly is provided on the plant tissue culture tank 10, which improves the flexibility of the installation and setting of the air intake structure 40, and is also beneficial to giving full play to the aeration effect of the air intake structure 40 in the culture tank, beneficial to increasing the dissolution rate of air, and can also promote the circulation of the culture solution in the culture tank, avoiding the entanglement and agglomeration of adventitious roots, thereby promoting the faster growth of adventitious roots.
[0076] Embodiment Two
[0077] As Figure 2 shown, in the embodiment of the present invention, an air intake structure 40 provided on the plant tissue culture tank 10 is introduced. The air intake structure 40 includes an air intake part 41, an aeration part 42, and a conduit 43 connecting the air intake part 41 and the aeration part 42. Among them, the conduit 43 is in the shape of a straight rod, and the air intake part 41 and the aeration part 42 are respectively connected to both ends of the conduit 43.
[0078] Particularly, a cavity 421 is provided at the center of the aeration part 42. The cavity 421 is communicated with the conduit 43, and micropores communicating from the outside of the aeration part 42 to the inside of the cavity 421 are provided on the cavity wall of the cavity 421. A large number of micropores are densely and evenly distributed on the entire cavity wall.
[0079] Among them, the aeration part 42 can be made of ceramic material or polymer material.
[0080] Preferably, the aeration part 42 is made of titanium alloy material, so that adventitious roots cannot adhere to the outer surface of the aeration part 42, thus avoiding the accumulation and decay of adventitious roots.
[0081] In this factual example, by densely arranging a large number of micropores on the side wall of the aeration part 42 and connecting all the micropores to the cavity 421 opened in the center of the aeration part 42, the gas entering the cavity 421 of the aeration part 42 from the air duct can be sprayed into the solution from all the micropores at the same time, so as to form a large number of small bubbles in the solution at the same time. The small bubbles move outward from the outer surface of the aeration part 42 and float upward, avoiding the aggregation of small bubbles into large bubbles, improving the contact and dissolution rate of the gas and the solution, and the upward movement of a large number of small bubbles dispersed in the solution promotes the circulating flow of the solution, further improving the dissolved oxygen effect.
[0082] As Figure 3 shown, in another embodiment of the present invention, an aeration part 42 of an air inlet structure 40 is introduced. In order to reduce the time for opening micropores on the aeration part 42 and ensure that the micropores on the aeration part 42 are more evenly distributed, and can better decompose air into small bubbles dissolved in the culture solution. The aeration part 42 is set to be composed of a large number of small particles with a diameter less than 1 mm stacked and sintered together to form a shell with a cavity 421 in the center.
[0083] Moreover, the small particles can be ceramic materials, polymer materials, or metal alloy materials.
[0084] Specifically, the small particles are set to be spherical. A large number of small particles are closely adhered and there are gaps between adjacent small particles. The gaps are arranged in sequence along the radial direction of the aeration part 42 and are connected to form micropores extending from the inner surface of the cavity wall to the outer surface of the cavity wall. In this way, the outer surface of the aeration part 42 is covered with micropores, similar to the mesh holes of a sieve.
[0085] Preferably, the small particles are made of metal titanium alloy, which can prevent adventitious roots from adhering to the outer surface of the aeration part 42.
[0086] In this embodiment, the aeration part 42 is set by the method of sintering and adhering small particles, so that the micropores formed on the aeration part 42 are more evenly distributed, thus being able to better decompose air into small bubbles dissolved in the culture solution, which is beneficial to increasing the dissolution rate of air in the culture solution.
[0087] As Figure 3 shown, in another embodiment of the present invention, an air inlet structure 40 that can be more conveniently installed on a culture tank is introduced.
[0088] Specifically, the aeration part 42 of the air intake mechanism is set to be long cylindrical, with one end of the aeration part 42 connected to the conduit 43. The central axis of the aeration part 42 is set to coincide with the central axis of the end of the conduit 43 and extend linearly away from the conduit 43. At the same time, the conduit 43 is in a long straight shape, so that the central axes of the aeration part 42 and the conduit 43 coincide.
[0089] In this embodiment, setting the aeration part 42 as long cylindrical and extending it outward from the end of the conduit 43 can reduce the diameter of the opening formed on the culture tank and increase the contact area between the air intake structure 40 and the culture solution, which is beneficial to fully dispersing and dissolving air in the culture solution.
[0090] As Figure 3 shown, in another embodiment of the present invention, an air intake structure 40 that can be more conveniently fixed is introduced. A seat plate 44 provided on the air intake structure 40 is located between the air intake part 41 and the aeration part 42, separating the air intake part 41 and the aeration part 42.
[0091] Specifically, the seat plate 44 is connected to the conduit 43 and extends radially outward along the conduit 43, forming a shape where the conduit 43 vertically passes through the center of the seat plate 44. In particular, the seat plate 44 is located between the two ends of the conduit 43, the aeration part 42 is distributed on one side of the seat plate 44, and the air intake part 41 is provided on the other side of the seat plate 44.
[0092] Preferably, the seat plate 44 is set as a circular flat plate, so that the air intake structure 40 can rotate around the conduit 43 during installation.
[0093] In this embodiment, by providing the seat plate 44 on the air intake structure 40, it is more convenient to install and connect the air intake structure 40 to the culture tank, and the air intake structure 40 can also rotate around the conduit 43, reducing the difficulty of the assembly work.
[0094] As Figure 3 shown, in another embodiment of the present invention, an air intake structure 40 with better air intake effect is introduced. In order to better dissolve air when it enters the culture solution, the conduit 43 of the air intake structure 40 is optimized and adjusted.
[0095] Specifically, the conduit 43 is set to include a long straight air intake section 431. The air intake section 431 extends at least a certain length on both sides of the seat plate 44, which is beneficial to better connecting and fixing the aeration part 42 and the air intake part 41 to the conduit 43.
[0096] The aeration part 42 and the air intake part 41 are respectively connected to the ends of the air intake section 431 that extend out of the side of the seat plate 44, so as to separate the aeration part 42 and the air intake part 41 on both sides of the seat plate 44.
[0097] In another embodiment of the present invention, in order to increase the position of the aeration part 42 in the culture tank after being installed in the culture tank, the conduit 43 is further provided with an air outlet section 432 arranged at an angle with the air inlet section 431.
[0098] One end of the air outlet section 432 is connected to the air inlet section 431, and the other end is connected to the aeration part 42. Specifically, the angle between the air outlet section 432 and the air inlet section 431 is set to be greater than 90°.
[0099] In another embodiment, in order to further widen the position range of the aeration part 42 in the culture tank in cooperation with the seat plate 44, the conduit 43 is further provided with a transition section 433 connected between the air inlet section 431 and the air outlet section 432.
[0100] Specifically, the transition section 433 is set to be parallel to the seat plate 44. One end of the transition section 433 is connected to the air inlet section 431, and the other end is connected to the air outlet section 432. The air outlet section 432 is inclined with the transition section 433 and extends away from the seat plate 44 from the transition section 433.
[0101] Preferably, the total length of the connected aeration part 42, air outlet section 432 and transition section 433 is set to be at least greater than the radius of the cross-section of the culture tank. In addition, the length of the aeration part 42 is greater than the sum of the lengths of the air outlet section 432 and the transition section 433.
[0102] In this embodiment, the optimized conduit 43 includes multiple straight sections and adjacent straight sections are arranged in a bent connection, so that when the air inlet structure 40 is installed in the culture tank, by rotating the air inlet structure 40, it is ensured that the aeration part 42 can be in more positions in the culture tank, increasing the hoverable position range of the aeration part 42 in the culture tank, which is beneficial to the bubbles to fully diffuse from the periphery of the aeration part 42 into the culture solution.
[0103] As Figure 2 、 Figure 3 shown, in another embodiment of the present invention, an air inlet structure 40 capable of quick connection is introduced. The air inlet part 41 of the air inlet structure 40 includes a quick-release joint 45.
[0104] Specifically, the quick-release joint 45 in the air inlet part 41 is arranged at the end of the conduit 43. The quick-release joint 45 is conical. And, the conical bottom surface of the quick-release joint 45 coincides with the end face of the air inlet of the conduit 43. The air inlet of the conduit 43 is located at the center of the bottom surface. The conical surface of the quick-release joint 45 faces the seat plate 44 and gradually contracts to the outer peripheral surface of the conduit 43.
[0105] As Figure 3 shown, in another embodiment of the present invention, a quick-release joint 45 with a sealed air inlet structure 40 is introduced.
[0106] The conical bottom surface of the quick-release joint 45 is provided with a sealing groove 451. The sealing groove 451 is recessed from the bottom surface towards the inside of the quick-release joint 45 and forms a notch on the bottom surface. In particular, the sealing groove 451 surrounds the outer periphery of the air inlet of the conduit 43.
[0107] As Figure 1 shown, the present invention also provides a plant tissue culture tank 10 having any one of the above air inlet structures 40. The bottom of the plant tissue culture tank 10 is arranged as an inverted cone. An interface 51 for connecting the air inlet structure 40 is provided on the side wall of the cone. The air inlet structure 40 is connected to the interface 51, and its aeration part 42 is located in the cavity 421 of the plant tissue culture tank 10, and the air inlet part 41 extends outside the plant tissue culture tank 10.
[0108] In this embodiment, by providing the above air inlet structure 40 in the plant tissue culture tank 10, when cultivating adventitious roots, a large number of small bubbles can be formed in the solution by air. The small bubbles move outward and upward from the aeration part 42, which promotes the circulation and flow of the nutrient solution. And a large number of small bubbles are dispersed in the solution, which further improves the contact and dissolution rate of the gas and the nutrient solution, provides stable conditions for the proliferation of adventitious roots, and is beneficial to improving the cultivation efficiency and quality of adventitious roots.
[0109] The above are only the preferred embodiments of the present invention, and do not impose any form of limitation on the present invention. Although the present invention has been disclosed above with the preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to the above-mentioned technical content within the scope of the technical solution of the present invention to make equivalent embodiments of equivalent changes. However, as long as it does not depart from the technical solution of the present invention, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention still fall within the scope of the technical solution of the present invention.
Claims
1. An air inlet structure installation assembly for a plant tissue culture tank, including an interface (51) opened on the tank wall (11) of the plant tissue culture tank, characterized in that, The intake structure (40) includes an intake part (41), an aeration part (42), and a conduit (43) connecting the intake part (41) and the aeration part (42). The conduit includes a long straight intake section and an outlet section disposed at an angle to the intake section. One end of the outlet section is connected to the intake section, and the other end is connected to the aeration part. It further includes: A seat plate (44) sleeved on the outer periphery of the intake section and fixedly connected to the outer periphery of the intake section. The intake section projects at least a certain length on both sides of the seat plate. A hub kit nested in the interface and connected to the interface by its outer peripheral surface. A pressing member (52); The seat plate and the hub kit are in clearance socket fit. The seat plate can be flexibly rotated when inserted into the central hole of the hub kit. The pressing member presses from the other side of the seat plate to clamp and fix the seat plate in the hub kit. The seat plate (44) separates the aeration part (42) and the intake part (41) inside and outside the plant tissue culture tank. When installing the intake structure, the seat plate can rotate at any angle relative to the hub kit.
2. The installation assembly of the intake structure of a plant tissue culture tank according to claim 1, characterized in that The pressing member (52) is annular. The pressing member (52) is sleeved on the seat plate (44) and abuts against the plate surface of the seat plate (44) facing the outside of the plant tissue culture tank with the edge of its ring hole, for clamping the intake structure (40) on the plant tissue culture tank.
3. The installation assembly of the intake structure of a plant tissue culture tank according to claim 2, characterized in that The hub kit (53) protrudes axially towards the inside and outside of the tank wall (11), and its inner peripheral surface is connected to the seat plate (44).
4. The installation assembly of the intake structure of a plant tissue culture tank according to claim 3, characterized in that One end of the hub kit (53) inside the tank wall (11) of the plant tissue culture tank is provided with a support ring (531), which extends radially from the inner peripheral surface of the hub kit (53) towards the center. The seat plate (44) is sleeved on the inner peripheral surface of the hub kit (53) from the outside of the tank wall (11) of the plant tissue culture tank and abuts against the support ring (531).
5. The intake structure installation assembly of a plant tissue culture tank according to claim 4, characterized in that, It further includes a sealing ring (54) provided between the seat plate (44) and the support ring (531) and clamped by the end face of the seat plate (44) facing the inside of the plant tissue culture tank and the support ring (531).
6. The air inlet structure installation assembly of a plant tissue culture tank according to claim 5, characterized in that, The support ring (531) is provided with a groove (532) with an opening facing the outside of the tank wall (11) of the plant tissue culture tank, and the sealing ring (54) is installed in the groove (532) by being pressed by the seat plate (44).
7. The installation assembly of the intake structure of a plant tissue culture tank according to claim 6, characterized in that The end face of the hub kit (53) protruding outside the tank wall (11) is provided with a connection hole (533) extending along its axis. The pressing member (52) can be connected to the end face of the hub kit (53) to clamp the seat plate (44) and is provided with a through hole (521) corresponding to the connection hole (533) for passing through and fixing screws.
8. The air inlet structure installation assembly of a plant tissue culture tank according to claim 4, characterized in that the inner circumferential surface of the hub kit (53) is a cylindrical surface, the seat plate (44) is spherical and has the same radius as the inner circumferential surface of the hub kit (53), and the pressing member (52) and the support ring (531) rotatably clamp the seat plate (44) in the hub kit (53).
9. The intake structure installation assembly of a plant tissue culture tank according to claim 8, characterized in that, One end of the pressing member (52) and the support ring (531) close to the inner circumferential surface of the hub kit (53) is provided with a spherical curved surface.
10. A plant tissue culture tank, characterized in that, The air inlet structure installation assembly according to any one of the above claims 1-9 is adopted.
Citation Information
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